Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,332,963 C→A 38.9% A154A (GCG→GCT)  mlaF ← ABC transporter maintaining OM lipid asymmetry, ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,332,9630CA38.9% 315.8 / 271.2 244A154A (GCG→GCT) mlaFABC transporter maintaining OM lipid asymmetry, ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (79/70);  new base A (52/43);  total (131/113)
Fisher's exact test for biased strand distribution p-value = 8.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 249 total aligned reads displayed.

GTGAGAAACCACCACACAAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTT  >  CP009273/3332816‑3333112
                                                                                                                                                   |                                                                                                                                                     
  gagaAACCACCACACAAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTg                                                                                                                                                   >  2:3709032/1‑150 (MQ=255)
     aaaCCACCACACAAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCAc                                                                                                                                                <  2:696632/150‑1 (MQ=255)
      aaCCACCACACAAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCAcg                                                                                                                                               >  1:3070789/1‑150 (MQ=255)
       accaccACACAAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCAcgc                                                                                                                                              >  1:3720187/1‑150 (MQ=255)
        ccaccaCACAAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCAcgcc                                                                                                                                             <  1:4017778/150‑1 (MQ=255)
         caccacACAAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCAcgccg                                                                                                                                            >  2:1132107/1‑150 (MQ=255)
          accacACAAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCAcgccgc                                                                                                                                           >  2:2495861/1‑150 (MQ=255)
            cacacaAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCgcgc                                                                                                                                         >  1:2744103/1‑150 (MQ=255)
               acaAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCAt                                                                                                                                      <  1:3425828/150‑1 (MQ=255)
                    tCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCAc                                                                                                                                 >  1:723340/1‑150 (MQ=255)
                    tCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCAc                                                                                                                                 >  2:3371187/1‑150 (MQ=255)
                     cACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCAcc                                                                                                                                <  1:3286326/150‑1 (MQ=255)
                      aCGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCg                                                                                                                               <  2:2411262/150‑1 (MQ=255)
                      aCGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCg                                                                                                                               <  2:1366519/150‑1 (MQ=255)
                       cGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCgg                                                                                                                              >  2:1612542/1‑150 (MQ=255)
                          ccAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATTATGAGATCCGGCTCCAGCGAAATCGCACGCGCCAGCGCTGCACGCCTCGCCATCCCACCGGaaa                                                                                                                           <  2:1968186/150‑1 (MQ=255)
                          ccAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGaaa                                                                                                                           <  2:3263915/150‑1 (MQ=255)
                          ccAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGaaa                                                                                                                           >  1:1801482/1‑150 (MQ=255)
                              cgcgcTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTc                                                                                                                       <  1:1859931/150‑1 (MQ=255)
                                 gcTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGa                                                                                                                    >  2:816558/1‑150 (MQ=255)
                                      tCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCa                                                                                                               >  1:2028546/1‑150 (MQ=255)
                                        aGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGTATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCAtt                                                                                                             <  2:3101616/150‑1 (MQ=255)
                                        aGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCAtt                                                                                                             >  1:588995/1‑150 (MQ=255)
                                         gCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTa                                                                                                            <  2:2128049/150‑1 (MQ=255)
                                         gCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAAAAAAAGGTTCATCAAACATGATGAGATCCGGCTCCATCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGTAGGCATTa                                                                                                            <  1:481081/150‑1 (MQ=255)
                                           tCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGt                                                                                                          >  2:3551237/1‑150 (MQ=255)
                                            cAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGtt                                                                                                         <  2:1335875/150‑1 (MQ=255)
                                            cAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGtt                                                                                                         <  1:366063/150‑1 (MQ=255)
                                             aGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGttt                                                                                                        <  2:3336158/150‑1 (MQ=255)
                                               aaaTCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAg                                                                                                      <  2:2560022/150‑1 (MQ=255)
                                               aaaTCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAg                                                                                                      >  1:4148478/1‑150 (MQ=255)
                                               aaaTCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAg                                                                                                      <  1:618591/150‑1 (MQ=255)
                                                aaTCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGc                                                                                                     <  2:3030419/150‑1 (MQ=255)
                                                 aTCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGcc                                                                                                    <  2:324887/150‑1 (MQ=255)
                                                 aTCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGcc                                                                                                    <  2:2289183/150‑1 (MQ=255)
                                                     gCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTc                                                                                                >  2:1865989/1‑150 (MQ=255)
                                                      cTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTcc                                                                                               <  1:1607404/150‑1 (MQ=255)
                                                       ttCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCa                                                                                              >  2:2880408/1‑150 (MQ=255)
                                                       ttCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCa                                                                                              <  1:717682/150‑1 (MQ=255)
                                                         cACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACg                                                                                            <  2:2513953/150‑1 (MQ=255)
                                                          aCCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGc                                                                                           >  1:206862/1‑150 (MQ=255)
                                                            cAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCAGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAg                                                                                         >  2:220851/1‑150 (MQ=255)
                                                             aGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGc                                                                                        >  1:2316773/1‑150 (MQ=255)
                                                                aCGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGcccc                                                                                     >  2:152514/1‑150 (MQ=255)
                                                                aCGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGcccc                                                                                     <  2:317622/150‑1 (MQ=255)
                                                                aCGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGcccc                                                                                     >  2:1716931/1‑150 (MQ=255)
                                                                  gCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCAc                                                                                   <  2:3576533/150‑1 (MQ=255)
                                                                      aTGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGcc                                                                               >  2:2625582/1‑150 (MQ=255)
                                                                       tGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCt                                                                              <  1:1035902/150‑1 (MQ=255)
                                                                        ggTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTc                                                                             >  2:873341/1‑150 (MQ=255)
                                                                         gTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTcc                                                                            >  1:3490951/1‑150 (MQ=255)
                                                                          tGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCa                                                                           >  1:1129559/1‑150 (MQ=255)
                                                                          tGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCa                                                                           >  1:1129278/1‑150 (MQ=255)
                                                                                 aTCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTtcat                                                                    <  2:1720059/150‑1 (MQ=255)
                                                                                 aTCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCAGTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTtcat                                                                    >  2:3904514/1‑150 (MQ=255)
                                                                                     tGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTtcatcatc                                                                >  1:2646584/1‑150 (MQ=255)
                                                                                     tGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTtcatcatc                                                                >  1:2337140/1‑150 (MQ=255)
                                                                                        ccAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCAcc                                                             <  1:880480/150‑1 (MQ=255)
                                                                                          aacaaAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGt                                                           >  2:743127/1‑150 (MQ=255)
                                                                                           acaaAATGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTa                                                          >  1:3646960/1‑150 (MQ=255)
                                                                                             aaaaGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACt                                                        <  2:1636103/150‑1 (MQ=255)
                                                                                             aaaaGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACt                                                        >  2:2034706/1‑150 (MQ=255)
                                                                                              aaaGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTa                                                       >  2:3534236/1‑150 (MQ=255)
                                                                                                 ggTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCAAGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTTTGc                                                    <  2:662923/150‑1 (MQ=255)
                                                                                                      atcaAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGcaacaa                                               >  1:2937427/1‑150 (MQ=255)
                                                                                                       tcaAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAAt                                              >  2:1264271/1‑150 (MQ=255)
                                                                                                        caAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATg                                             <  1:2386228/150‑1 (MQ=255)
                                                                                                        caAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATg                                             >  2:595948/1‑150 (MQ=255)
                                                                                                        caAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATg                                             <  2:3124740/150‑1 (MQ=255)
                                                                                                           aCATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGgcg                                          >  1:269468/1‑150 (MQ=255)
                                                                                                            cATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGgcgc                                         >  2:1597766/1‑150 (MQ=255)
                                                                                                            cATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGgcgc                                         >  2:2102524/1‑150 (MQ=255)
                                                                                                               gatgaGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCggg                                      <  2:4028005/150‑1 (MQ=255)
                                                                                                                atgaGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGa                                     <  1:314750/150‑1 (MQ=255)
                                                                                                                    gaTCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGtt                                 >  2:1190093/1‑150 (MQ=255)
                                                                                                                     aTCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTg                                >  1:1879278/1‑150 (MQ=255)
                                                                                                                     aTCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTg                                >  2:3496720/1‑150 (MQ=255)
                                                                                                                          gCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTAt                           <  2:3701948/150‑1 (MQ=255)
                                                                                                                           cTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGACGTTGGGTATg                          >  2:3817056/1‑150 (MQ=255)
                                                                                                                            tCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGt                         <  1:1292593/150‑1 (MQ=255)
                                                                                                                             ccAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGtt                        >  1:1225489/1‑150 (MQ=255)
                                                                                                                             ccAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGtt                        >  1:1568428/1‑150 (MQ=255)
                                                                                                                              cAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTc                       <  1:1557900/150‑1 (MQ=255)
                                                                                                                               aGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTcg                      <  2:1074444/150‑1 (MQ=255)
                                                                                                                               aGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTcg                      >  2:2683431/1‑150 (MQ=255)
                                                                                                                               aGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTcg                      <  2:1658852/150‑1 (MQ=255)
                                                                                                                               aGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTcg                      <  1:1438991/150‑1 (MQ=255)
                                                                                                                                 cgcAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTcgcg                    <  2:2158026/150‑1 (MQ=255)
                                                                                                                                  gcAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCATGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCgcgc                   >  2:3176905/1‑150 (MQ=255)
                                                                                                                                      tCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTg               >  1:2811330/1‑150 (MQ=255)
                                                                                                                                      tCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTg               <  2:2320080/150‑1 (MQ=255)
                                                                                                                                       cGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTgg              <  1:2534463/150‑1 (MQ=255)
                                                                                                                                       cGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTgg              >  2:1988606/1‑150 (MQ=255)
                                                                                                                                        gCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGa             <  1:2267086/150‑1 (MQ=255)
                                                                                                                                         cACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGAt            >  1:1776140/1‑150 (MQ=255)
                                                                                                                                             cgcCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGc        >  2:906251/1‑150 (MQ=255)
                                                                                                                                              gcCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCg       <  2:1048496/150‑1 (MQ=255)
                                                                                                                                                cAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGAc     >  1:2491543/1‑150 (MQ=255)
                                                                                                                                                 aGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCAGTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACg    >  2:20621/1‑150 (MQ=255)
                                                                                                                                                  gcgcTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGt   <  2:188355/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GTGAGAAACCACCACACAAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTT  >  CP009273/3332816‑3333112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A