Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F159 I0 R1 879 305.9 9588012 96.6% 9262019 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,332,963 C→A 28.7% A154A (GCG→GCT)  mlaF ← ABC transporter maintaining OM lipid asymmetry, ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,332,9630CA28.7% 673.9 / 250.3 319A154A (GCG→GCT) mlaFABC transporter maintaining OM lipid asymmetry, ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (109/118);  new base A (31/60);  total (140/179)
Fisher's exact test for biased strand distribution p-value = 2.50e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 326 total aligned reads displayed.

CGTGAGAAACCACCACACAAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTT  >  CP009273/3332815‑3333112
                                                                                                                                                    |                                                                                                                                                     
cGTGAGAAACCACCACACAAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAgcg                                                                                                                                                      <  2:3577304/150‑1 (MQ=255)
  tGAGAAACCACCACACAAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCt                                                                                                                                                    <  2:2829669/150‑1 (MQ=255)
   gagaAACCACCACACAAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTg                                                                                                                                                   >  2:2913983/1‑150 (MQ=255)
     gaAACCACCACACAAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCa                                                                                                                                                 >  1:2036466/1‑150 (MQ=255)
         ccaccaCACAAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCAcgcc                                                                                                                                             <  2:3124462/150‑1 (MQ=255)
           accacACAAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCAcgccgc                                                                                                                                           >  1:2212996/1‑150 (MQ=255)
              acacaAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGcc                                                                                                                                        <  2:1938341/150‑1 (MQ=255)
                  aaGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATcc                                                                                                                                    >  2:2266217/1‑150 (MQ=255)
                    gTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCa                                                                                                                                  <  2:738224/150‑1 (MQ=255)
                     tctcGCCCAGCGCGCTGTTCAGCTCATAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCAc                                                                                                                                 <  2:938293/147‑1 (MQ=255)
                     tCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCAc                                                                                                                                 <  1:2854836/150‑1 (MQ=255)
                       aCGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCg                                                                                                                               <  1:1739425/150‑1 (MQ=255)
                       aCGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCg                                                                                                                               <  2:112282/150‑1 (MQ=255)
                          cccAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGaa                                                                                                                            >  2:4035092/1‑150 (MQ=255)
                          cccAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGaa                                                                                                                            >  2:3022684/1‑150 (MQ=255)
                            cAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAg                                                                                                                          >  1:1708885/1‑150 (MQ=255)
                             aGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGt                                                                                                                         >  1:3248414/1‑150 (MQ=255)
                               cgcgcTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTc                                                                                                                       >  2:2825794/1‑150 (MQ=255)
                               cgcgcTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTc                                                                                                                       <  2:4573744/150‑1 (MQ=255)
                               cgcgcTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTc                                                                                                                       <  2:2534557/150‑1 (MQ=255)
                                   cTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGaa                                                                                                                   <  1:2102417/150‑1 (MQ=255)
                                     gTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAgg                                                                                                                 <  2:2163540/150‑1 (MQ=255)
                                        cAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCAt                                                                                                              <  2:1246544/150‑1 (MQ=255)
                                        cAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCCCCGGAAAGTTCAGAAGGCAt                                                                                                              <  2:823577/150‑1 (MQ=255)
                                          gCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTa                                                                                                            <  1:1932160/150‑1 (MQ=255)
                                          gCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTa                                                                                                            <  2:1064926/150‑1 (MQ=255)
                                          gCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTa                                                                                                            <  1:2663487/150‑1 (MQ=255)
                                            tcggAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGt                                                                                                          <  2:3477644/147‑1 (MQ=255)
                                             cAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGtt                                                                                                         <  2:4676522/150‑1 (MQ=255)
                                             cAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGtt                                                                                                         <  1:2560854/150‑1 (MQ=255)
                                             cAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGtt                                                                                                         <  1:3552496/150‑1 (MQ=255)
                                              aGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGttt                                                                                                        <  1:1909004/150‑1 (MQ=255)
                                                   tCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCg                                                                                                   <  1:3957401/150‑1 (MQ=255)
                                                   tCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCg                                                                                                   <  1:1551512/150‑1 (MQ=255)
                                                    cAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGc                                                                                                  >  2:3862860/1‑150 (MQ=255)
                                                         tCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCAc                                                                                             >  1:2810720/1‑150 (MQ=255)
                                                         tCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCAc                                                                                             >  1:2367310/1‑150 (MQ=255)
                                                         tCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCAGTAGTTTAGCCGCTCCAc                                                                                             >  2:2629785/1‑150 (MQ=255)
                                                          cACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACg                                                                                            <  2:428288/150‑1 (MQ=255)
                                                            ccAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCa                                                                                          >  2:1328115/1‑150 (MQ=255)
                                                              aGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGc                                                                                        <  2:1541128/150‑1 (MQ=255)
                                                               gTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGcc                                                                                       <  2:451239/150‑1 (MQ=255)
                                                                 aCGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGcccc                                                                                     >  2:4199327/1‑150 (MQ=255)
                                                                  cGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCa                                                                                    >  1:3385586/1‑150 (MQ=255)
                                                                  cGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCa                                                                                    <  1:1979996/150‑1 (MQ=255)
                                                                    cccATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACg                                                                                  <  1:3658166/150‑1 (MQ=255)
                                                                      cATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATGAGTTTAGCCGCTCCACGCAGCCCCACGGc                                                                                >  2:4295849/1‑150 (MQ=255)
                                                                       aTGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGcc                                                                               >  1:4466770/1‑150 (MQ=255)
                                                                       aTGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGcc                                                                               >  1:674680/1‑150 (MQ=255)
                                                                        tGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCt                                                                              >  1:4393101/1‑150 (MQ=255)
                                                                        tGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCt                                                                              <  2:882265/150‑1 (MQ=255)
                                                                         ggTGATGGGATCTTGCCCAACAAATGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGTGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCTTTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTc                                                                             <  1:4027775/150‑1 (MQ=255)
                                                                         ggTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTc                                                                             <  2:538148/150‑1 (MQ=255)
                                                                          gTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTcc                                                                            <  1:296047/150‑1 (MQ=255)
                                                                           tGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCa                                                                           <  2:950163/150‑1 (MQ=255)
                                                                                  aTCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTtcat                                                                    <  1:2255646/150‑1 (MQ=255)
                                                                                    cTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTtcatca                                                                  <  2:3247342/150‑1 (MQ=255)
                                                                                         ccAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCAcc                                                             <  1:88328/150‑1 (MQ=255)
                                                                                         ccAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCAcc                                                             <  1:2483484/150‑1 (MQ=255)
                                                                                           aacaaAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGt                                                           >  2:1781013/1‑150 (MQ=255)
                                                                                           aacaaAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGt                                                           >  2:39790/1‑150 (MQ=255)
                                                                                            acaaAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTa                                                          <  1:1661490/150‑1 (MQ=255)
                                                                                             caaAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTAc                                                         <  2:1708885/150‑1 (MQ=255)
                                                                                               aaaGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTa                                                       >  1:1413645/1‑150 (MQ=255)
                                                                                               aaaGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTa                                                       >  2:1773755/1‑150 (MQ=255)
                                                                                                aaGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTAt                                                      >  2:2062381/1‑150 (MQ=255)
                                                                                                  ggTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGc                                                    >  1:2416811/1‑150 (MQ=255)
                                                                                                   gTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGca                                                   >  2:4779934/1‑150 (MQ=255)
                                                                                                     tcatcaAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGcaac                                                 <  1:2825857/150‑1 (MQ=255)
                                                                                                     tcatcaAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGcaac                                                 <  1:4786396/150‑1 (MQ=255)
                                                                                                       atcaAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGcaacaa                                               >  1:1591189/1‑150 (MQ=255)
                                                                                                         caAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATg                                             <  1:3741575/150‑1 (MQ=255)
                                                                                                              atgatgAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGcgcg                                        <  1:4074792/150‑1 (MQ=255)
                                                                                                              atgatgAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGcgcg                                        <  2:4445252/150‑1 (MQ=255)
                                                                                                                gatgaGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCggg                                      >  1:101166/1‑150 (MQ=255)
                                                                                                                 atgaGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGa                                     <  2:3944868/150‑1 (MQ=255)
                                                                                                                     gaTCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGtt                                 <  1:1491567/150‑1 (MQ=255)
                                                                                                                     gaTCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGtt                                 <  2:3385586/150‑1 (MQ=255)
                                                                                                                        ccGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTggg                              <  1:961158/150‑1 (MQ=255)
                                                                                                                        ccGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTggg                              <  2:2035802/150‑1 (MQ=255)
                                                                                                                         cGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGt                             >  2:1187086/1‑150 (MQ=255)
                                                                                                                          ggCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTa                            >  1:3497025/1‑150 (MQ=255)
                                                                                                                           gCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTAt                           <  1:429936/150‑1 (MQ=255)
                                                                                                                           gCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTAt                           <  1:1466837/150‑1 (MQ=255)
                                                                                                                            cTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTCTGCAACAATGGCGCGGGAAGTTGGGTATg                          >  1:3247624/1‑150 (MQ=255)
                                                                                                                            cTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATg                          <  1:1314787/150‑1 (MQ=255)
                                                                                                                              ccAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGCAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGtt                        <  1:4760989/150‑1 (MQ=255)
                                                                                                                              ccAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGACGTTGGGTATGtt                        >  1:571532/1‑150 (MQ=255)
                                                                                                                              ccAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGtt                        <  1:4611487/150‑1 (MQ=255)
                                                                                                                               cAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTc                       >  1:3870606/1‑150 (MQ=255)
                                                                                                                               cAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTc                       <  1:3331530/150‑1 (MQ=255)
                                                                                                                                    cAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCa                  >  1:2988563/1‑150 (MQ=255)
                                                                                                                                          cACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGAt            <  2:3003630/150‑1 (MQ=255)
                                                                                                                                          cACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGAt            >  2:1490316/1‑150 (MQ=255)
                                                                                                                                            cgcgCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAg          <  1:1232180/150‑1 (MQ=255)
                                                                                                                                                ccAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGa      >  1:4627683/1‑150 (MQ=255)
                                                                                                                                                 cAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGAc     <  1:1187086/150‑1 (MQ=255)
                                                                                                                                                   gcgcTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGt   <  2:1654186/150‑1 (MQ=255)
                                                                                                                                                    cgcTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGtt  <  2:1017714/150‑1 (MQ=255)
                                                                                                                                                    cgcTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGtt  <  2:4127716/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGTGAGAAACCACCACACAAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTT  >  CP009273/3332815‑3333112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A