Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,437,010 C→A 12.1% V149V (GTG→GTT)  secY ← preprotein translocase membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,437,0100CA12.1% 1029.8 / 75.4 304V149V (GTG→GTT) secYpreprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (106/161);  new base A (22/15);  total (128/176)
Fisher's exact test for biased strand distribution p-value = 3.20e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 306 total aligned reads displayed.

AGTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTG  >  CP009273/3436861‑3437158
                                                                                                                                                     |                                                                                                                                                    
 gTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCa                                                                                                                                                     >  2:3826746/1‑150 (MQ=255)
  tCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCAc                                                                                                                                                    >  1:4044365/1‑150 (MQ=255)
  tCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCAc                                                                                                                                                    >  1:4378068/1‑150 (MQ=255)
    ccGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCa                                                                                                                                                  <  2:4528760/150‑1 (MQ=255)
     cgcgACAATACCGGCGTAGATAATGATTGAAATAGCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAg                                                                                                                                                 <  2:4134673/150‑1 (MQ=255)
     cgcgACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAg                                                                                                                                                 <  1:957147/150‑1 (MQ=255)
     cgcgACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGAATTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAg                                                                                                                                                 <  1:1699188/150‑1 (MQ=255)
      gcgACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAgg                                                                                                                                                <  1:849163/150‑1 (MQ=255)
      gcgACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAgg                                                                                                                                                <  1:3158276/150‑1 (MQ=255)
       cgACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGc                                                                                                                                               >  2:739259/1‑150 (MQ=255)
             tACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCa                                                                                                                                         <  1:2375440/150‑1 (MQ=255)
                 ggCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATAcc                                                                                                                                     >  1:1191805/1‑150 (MQ=255)
                 ggCGAAGATAATGAATGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATAACCAGGCCTTGCATAcc                                                                                                                                     <  2:1893954/150‑1 (MQ=255)
                   cGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAg                                                                                                                                   <  2:2407103/150‑1 (MQ=255)
                      aGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCa                                                                                                                                <  2:2847092/150‑1 (MQ=255)
                        aTAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGTTTAATCACCAGGCCTTGCATTCCTGGCata                                                                                                                              <  2:3646975/150‑1 (MQ=255)
                          aaTGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATAtt                                                                                                                            <  1:669488/150‑1 (MQ=255)
                            tGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTGAATCACCAGGCCTTGCATACCAGGCATATTCg                                                                                                                          <  1:2033864/150‑1 (MQ=255)
                             gATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCgg                                                                                                                         >  1:2403935/1‑150 (MQ=255)
                             gATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCgg                                                                                                                         >  2:3600245/1‑150 (MQ=255)
                             gATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCgg                                                                                                                         <  1:3405492/150‑1 (MQ=255)
                             gATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCgg                                                                                                                         <  2:4361605/150‑1 (MQ=255)
                              aTTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGc                                                                                                                        >  1:3836286/1‑150 (MQ=255)
                              aTTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGc                                                                                                                        >  2:140923/1‑150 (MQ=255)
                              aTTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCAAATTCGGc                                                                                                                        >  2:2444582/1‑150 (MQ=255)
                               ttGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCa                                                                                                                       <  2:1246867/150‑1 (MQ=255)
                                  aaaTACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGAc                                                                                                                    <  1:1948966/150‑1 (MQ=255)
                                   aaTACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGAcc                                                                                                                   >  1:561746/1‑150 (MQ=255)
                                   aaTACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGAcc                                                                                                                   >  1:564996/1‑150 (MQ=255)
                                    aTACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCg                                                                                                                  >  2:1219215/1‑150 (MQ=255)
                                            gCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAAt                                                                                                          <  1:1308637/150‑1 (MQ=255)
                                               gATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATAcc                                                                                                       >  1:2398305/1‑150 (MQ=255)
                                                  aCCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGAt                                                                                                    <  1:1926421/150‑1 (MQ=255)
                                                   ccTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATc                                                                                                   <  1:747704/150‑1 (MQ=255)
                                                      cGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGAc                                                                                                >  1:3467753/1‑150 (MQ=255)
                                                       gTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACt                                                                                               >  2:1442621/1‑150 (MQ=255)
                                                       gTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACt                                                                                               <  2:2379798/150‑1 (MQ=255)
                                                       gTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACt                                                                                               <  2:2379927/150‑1 (MQ=255)
                                                         tCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTgg                                                                                             >  1:4521304/1‑150 (MQ=255)
                                                         tCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTgg                                                                                             >  2:2583334/1‑150 (MQ=255)
                                                               aTCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATAtt                                                                                       <  2:536503/150‑1 (MQ=255)
                                                                tCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTg                                                                                      <  2:3411464/150‑1 (MQ=255)
                                                                 cTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGc                                                                                     >  2:4250347/1‑150 (MQ=255)
                                                                  tGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGCCTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGcc                                                                                    <  1:1131827/150‑1 (MQ=255)
                                                                   gTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCa                                                                                   >  2:832795/1‑150 (MQ=255)
                                                                   gTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCa                                                                                   <  2:3007888/150‑1 (MQ=255)
                                                                       gCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCAc                                                                               >  1:93544/1‑150 (MQ=255)
                                                                           aaCCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTATAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCTGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCaga                                                                           >  1:2992527/1‑150 (MQ=255)
                                                                           aaCCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCaga                                                                           >  1:1057074/1‑150 (MQ=255)
                                                                            aCCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTACTCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCagag                                                                          <  2:3009621/150‑1 (MQ=255)
                                                                            aCCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCagag                                                                          <  1:3625706/150‑1 (MQ=255)
                                                                            aCCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCagag                                                                          <  1:3998375/150‑1 (MQ=255)
                                                                              cacaTCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTa                                                                        <  1:323517/150‑1 (MQ=255)
                                                                               acaTCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTAc                                                                       >  1:3321638/1‑150 (MQ=255)
                                                                               acaTCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTAc                                                                       >  2:422517/1‑150 (MQ=255)
                                                                                caTCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTAcc                                                                      <  1:530866/150‑1 (MQ=255)
                                                                                 aTCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCg                                                                     >  1:707015/1‑150 (MQ=255)
                                                                                    aGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAg                                                                  >  2:125120/1‑150 (MQ=255)
                                                                                     ggAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGc                                                                 <  2:1723869/150‑1 (MQ=255)
                                                                                       aaCATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCgg                                                               >  2:2031489/1‑150 (MQ=255)
                                                                                          aTGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGgtg                                                            >  2:1848373/1‑150 (MQ=255)
                                                                                              ttCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACt                                                        >  1:3068787/1‑150 (MQ=255)
                                                                                                ccTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTgg                                                      <  2:1343417/150‑1 (MQ=255)
                                                                                                ccTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTgg                                                      <  1:2606471/150‑1 (MQ=255)
                                                                                                ccTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTgg                                                      <  1:1400285/150‑1 (MQ=255)
                                                                                                   gtgACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTg                                                   <  1:2231030/150‑1 (MQ=255)
                                                                                                     gACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGAt                                                 >  2:374953/1‑150 (MQ=255)
                                                                                                      aCCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATc                                                <  2:1817888/150‑1 (MQ=255)
                                                                                                      aCCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATc                                                <  2:2522862/150‑1 (MQ=255)
                                                                                                      aCCAGACTTACAACAGCGGCGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATc                                                >  2:4296091/1‑150 (MQ=255)
                                                                                                       ccAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCt                                               <  2:1575497/150‑1 (MQ=255)
                                                                                                        cAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCtt                                              <  2:3259340/150‑1 (MQ=255)
                                                                                                             ttACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTacgac                                         <  2:2277122/150‑1 (MQ=255)
                                                                                                                 aacaGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGAcc                                     >  2:1109029/1‑150 (MQ=255)
                                                                                                                 aacaGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGAcc                                     >  2:1029997/1‑150 (MQ=255)
                                                                                                                   caGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAg                                   <  1:1402104/150‑1 (MQ=255)
                                                                                                                     gCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGAc                                 <  1:4416900/150‑1 (MQ=255)
                                                                                                                           aaGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCct                           <  2:3462936/150‑1 (MQ=255)
                                                                                                                            aGTAGAATGCAAAGCCCGGGTTACTCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAAGACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCctt                          <  2:3471659/150‑1 (MQ=255)
                                                                                                                            aGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCctt                          >  1:2852917/1‑150 (MQ=255)
                                                                                                                            aGTAGAATGCAAAGCCCGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCctt                          >  1:3709786/1‑150 (MQ=255)
                                                                                                                             gTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCcttc                         >  1:215916/1‑150 (MQ=255)
                                                                                                                              tAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCcttct                        <  2:755400/150‑1 (MQ=255)
                                                                                                                              tAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCcttct                        <  2:1191805/150‑1 (MQ=255)
                                                                                                                              tAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACAGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCcttct                        <  1:3411977/150‑1 (MQ=255)
                                                                                                                                gAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCttcttt                      >  2:2253251/1‑150 (MQ=255)
                                                                                                                                 aaTGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCttctttc                     <  1:233159/150‑1 (MQ=255)
                                                                                                                                    gCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTa                  <  1:3860683/150‑1 (MQ=255)
                                                                                                                                    gCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTa                  <  1:1442621/150‑1 (MQ=255)
                                                                                                                                     cAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTaa                 >  2:1006017/1‑150 (MQ=255)
                                                                                                                                      aaaGCCCGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAAt                >  2:275510/1‑150 (MQ=255)
                                                                                                                                       aaGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAAtt               >  1:4449868/1‑150 (MQ=255)
                                                                                                                                            cGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGc          >  1:3952490/1‑150 (MQ=255)
                                                                                                                                             gggTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGcc         <  2:1826702/150‑1 (MQ=255)
                                                                                                                                                ttAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAAc      <  2:2379568/150‑1 (MQ=255)
                                                                                                                                                 tAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACg     <  2:3874535/150‑1 (MQ=255)
                                                                                                                                                  aaTCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGt    <  2:3178461/150‑1 (MQ=255)
                                                                                                                                                   aTCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGtt   <  2:646834/150‑1 (MQ=255)
                                                                                                                                                    tCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTg  <  2:3960646/150‑1 (MQ=255)
                                                                                                                                                    tCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTg  >  1:3180781/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AGTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTG  >  CP009273/3436861‑3437158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A