Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F159 I0 R1 879 305.9 9588012 96.6% 9262019 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,437,010 C→A 9.7% V149V (GTG→GTT)  secY ← preprotein translocase membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,437,0100CA 9.7% 1300.5 / 73.7 356V149V (GTG→GTT) secYpreprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (163/158);  new base A (19/16);  total (182/174)
Fisher's exact test for biased strand distribution p-value = 7.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 366 total aligned reads displayed.

AGTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGG  >  CP009273/3436861‑3437159
                                                                                                                                                     |                                                                                                                                                     
aGTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATc                                                                                                                                                       >  2:843021/1‑150 (MQ=255)
 gTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCa                                                                                                                                                      >  1:2275145/1‑150 (MQ=255)
   cccGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCAcc                                                                                                                                                    >  1:2791065/1‑150 (MQ=255)
     cgcgACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAg                                                                                                                                                  <  2:1237366/150‑1 (MQ=255)
        gACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGcc                                                                                                                                               <  1:2935072/150‑1 (MQ=255)
          cAATACCGGCGAAGATAATGATAGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCtt                                                                                                                                             <  2:390761/150‑1 (MQ=255)
              aCCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATAACCAGGCCTTGCAt                                                                                                                                         <  1:4054088/150‑1 (MQ=255)
                 ggCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATAcc                                                                                                                                      >  1:2024642/1‑150 (MQ=255)
                  gCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCa                                                                                                                                     <  1:3617404/150‑1 (MQ=255)
                   cGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACTGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAg                                                                                                                                    <  1:561591/150‑1 (MQ=255)
                    gAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAgg                                                                                                                                   >  2:3867945/1‑150 (MQ=255)
                      aGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATAACCAGGCCTTGCATACCAGGCa                                                                                                                                 >  2:132969/1‑150 (MQ=255)
                       gATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCat                                                                                                                                <  1:2140674/150‑1 (MQ=255)
                       gATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATAACCAGGCCTTGCATACCAGGCat                                                                                                                                >  2:3161952/1‑150 (MQ=255)
                         tAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCatat                                                                                                                              <  2:2385103/150‑1 (MQ=255)
                          aaTGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATAtt                                                                                                                             >  1:4120970/1‑150 (MQ=255)
                          aaTGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATAtt                                                                                                                             <  1:3862909/150‑1 (MQ=255)
                            tGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCg                                                                                                                           <  2:1876755/150‑1 (MQ=255)
                            tGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCg                                                                                                                           <  2:3111443/150‑1 (MQ=255)
                             gATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCgg                                                                                                                          >  2:3324415/1‑150 (MQ=255)
                               ttGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCa                                                                                                                        <  1:2543013/150‑1 (MQ=255)
                                      aCCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGt                                                                                                                 <  1:258576/150‑1 (MQ=255)
                                       ccGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTa                                                                                                                <  1:3880449/150‑1 (MQ=255)
                                        cGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAg                                                                                                               >  1:608503/1‑150 (MQ=255)
                                         gTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGc                                                                                                              >  1:2458172/1‑150 (MQ=255)
                                           tGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCaa                                                                                                            <  1:4707848/150‑1 (MQ=255)
                                             ccGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATa                                                                                                          >  2:738104/1‑150 (MQ=255)
                                              cGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATAc                                                                                                         >  2:2685123/1‑150 (MQ=255)
                                              cGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATAc                                                                                                         >  1:2848340/1‑150 (MQ=255)
                                                aTACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCg                                                                                                       <  2:4052080/150‑1 (MQ=255)
                                                aTACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCg                                                                                                       >  2:4208044/1‑150 (MQ=255)
                                                 tACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCACTACCGa                                                                                                      >  1:1006488/1‑150 (MQ=255)
                                                   ccTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATc                                                                                                    <  1:4257307/150‑1 (MQ=255)
                                                   ccTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATc                                                                                                    <  1:4661639/150‑1 (MQ=255)
                                                    cTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCg                                                                                                   >  2:582912/1‑150 (MQ=255)
                                                    cTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCg                                                                                                   >  2:4203605/1‑150 (MQ=255)
                                                      cGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGAc                                                                                                 <  1:3996296/150‑1 (MQ=255)
                                                       gTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACt                                                                                                >  1:1814365/1‑150 (MQ=255)
                                                           aGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGaa                                                                                            >  2:511315/1‑150 (MQ=255)
                                                            gTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAat                                                                                           >  2:2067689/1‑150 (MQ=255)
                                                              aaTCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAatat                                                                                         >  2:1827092/1‑150 (MQ=255)
                                                                  tGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGcc                                                                                     >  1:1766488/1‑150 (MQ=255)
                                                                   gTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCa                                                                                    <  1:1041171/150‑1 (MQ=255)
                                                                   gTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCa                                                                                    <  2:4416842/150‑1 (MQ=255)
                                                                   gTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCa                                                                                    >  2:1597846/1‑150 (MQ=255)
                                                                   gTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCa                                                                                    <  1:3442759/150‑1 (MQ=255)
                                                                   gTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCa                                                                                    <  1:944151/150‑1 (MQ=255)
                                                                    ttCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAg                                                                                   <  2:2244662/150‑1 (MQ=255)
                                                                     tCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGc                                                                                  >  2:2944345/1‑150 (MQ=255)
                                                                      cGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCa                                                                                 <  1:1251840/150‑1 (MQ=255)
                                                                      cGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCa                                                                                 >  2:2851810/1‑150 (MQ=255)
                                                                        cccAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCAcc                                                                               >  2:1517453/1‑150 (MQ=255)
                                                                              cacaTCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTa                                                                         >  1:436805/1‑150 (MQ=255)
                                                                                 aTCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCg                                                                      <  1:2679284/150‑1 (MQ=255)
                                                                                   cAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTa                                                                    >  1:2163314/1‑150 (MQ=255)
                                                                                   cAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTa                                                                    >  1:3066667/1‑150 (MQ=255)
                                                                                   cAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTa                                                                    >  1:1678618/1‑150 (MQ=255)
                                                                                   cAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTa                                                                    >  1:2083707/1‑150 (MQ=255)
                                                                                   cAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTa                                                                    >  1:1476935/1‑150 (MQ=255)
                                                                                    aGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAg                                                                   <  1:1977693/150‑1 (MQ=255)
                                                                                         cATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGgt                                                              <  1:3335507/150‑1 (MQ=255)
                                                                                          aTGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGgtg                                                             >  2:424430/1‑150 (MQ=255)
                                                                                          aTGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGgtg                                                             >  1:4432662/1‑150 (MQ=255)
                                                                                            ggTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTa                                                           <  1:3205865/150‑1 (MQ=255)
                                                                                             gTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTAc                                                          >  2:2650572/1‑150 (MQ=255)
                                                                                               tCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTg                                                        <  1:3572043/150‑1 (MQ=255)
                                                                                                ccTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTgg                                                       <  2:875802/150‑1 (MQ=255)
                                                                                                     gACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGAt                                                  <  1:4027385/150‑1 (MQ=255)
                                                                                                        cAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCtt                                               <  2:1103141/150‑1 (MQ=255)
                                                                                                         aGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTa                                              <  1:84287/150‑1 (MQ=255)
                                                                                                          gACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTac                                             >  1:756255/1‑150 (MQ=255)
                                                                                                           aCTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTacg                                            >  2:3757990/1‑150 (MQ=255)
                                                                                                               acaacaGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTAcgacga                                        >  1:3529933/1‑150 (MQ=255)
                                                                                                               acaacaGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTAcgacga                                        >  1:4156210/1‑150 (MQ=255)
                                                                                                                    aGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGa                                   <  1:4662263/150‑1 (MQ=255)
                                                                                                                     gCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGAc                                  <  2:4709395/150‑1 (MQ=255)
                                                                                                                       ggTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTc                                >  1:603345/1‑150 (MQ=255)
                                                                                                                          gAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTcccc                             <  1:1941115/150‑1 (MQ=255)
                                                                                                                            aGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCctt                           <  2:4593645/150‑1 (MQ=255)
                                                                                                                              tAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCcttct                         >  2:2351072/1‑150 (MQ=255)
                                                                                                                               aGAATGCAAAGCCCGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCcttctt                        <  1:3818934/150‑1 (MQ=255)
                                                                                                                                 aaTGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCttctttc                      >  2:3816138/1‑150 (MQ=255)
                                                                                                                                 aaTGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCttctttc                      >  2:2462948/1‑150 (MQ=255)
                                                                                                                                 aaTGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCttctttc                      >  2:1736587/1‑150 (MQ=255)
                                                                                                                                  aTGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCttctttct                     <  1:478131/150‑1 (MQ=255)
                                                                                                                                  aTGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCttctttct                     >  1:1286569/1‑150 (MQ=255)
                                                                                                                                     caatgaccGTGTTACTCACCTGGCGTTGCATACCTGGCATTTTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATATTACGACGACCAGTCTCCCCTTCTTTCTTaa                  <  1:2393968/144‑1 (MQ=255)
                                                                                                                                      aaaGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAAt                 <  1:522460/150‑1 (MQ=255)
                                                                                                                                      aaaGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAAt                 >  2:2981732/1‑150 (MQ=255)
                                                                                                                                       aaGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAAtt                >  2:356214/1‑150 (MQ=255)
                                                                                                                                        aGCCCGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAAttt               <  2:201734/150‑1 (MQ=255)
                                                                                                                                          cccGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCt             <  1:669982/150‑1 (MQ=255)
                                                                                                                                          cccGGGTTAATCACCAGGCCTTGCATACCAGGCATAAACCCCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCt             >  2:3025672/1‑150 (MQ=255)
                                                                                                                                          cccGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCt             <  1:1438241/150‑1 (MQ=255)
                                                                                                                                            cGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGc           <  2:1656888/150‑1 (MQ=255)
                                                                                                                                                ttAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAAc       >  1:4587254/1‑150 (MQ=255)
                                                                                                                                                ttAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAAc       <  1:967426/150‑1 (MQ=255)
                                                                                                                                                 tAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACg      <  1:1535715/150‑1 (MQ=255)
                                                                                                                                                  aaTCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGt     >  2:1146311/1‑150 (MQ=255)
                                                                                                                                                   aTCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGtt    <  2:3413369/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGG  >  CP009273/3436861‑3437159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A