Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,437,010 C→A 26.8% V149V (GTG→GTT)  secY ← preprotein translocase membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,437,0100CA26.8% 629.1 / 204.9 282V149V (GTG→GTT) secYpreprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (109/97);  new base A (35/41);  total (144/138)
Fisher's exact test for biased strand distribution p-value = 3.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 290 total aligned reads displayed.

AGTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGG  >  CP009273/3436861‑3437159
                                                                                                                                                     |                                                                                                                                                     
aGTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATc                                                                                                                                                       <  2:352300/150‑1 (MQ=255)
aGTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATc                                                                                                                                                       <  1:2748080/150‑1 (MQ=255)
 gTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCa                                                                                                                                                      <  1:3264661/150‑1 (MQ=255)
  tCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCAc                                                                                                                                                     >  2:1806432/1‑150 (MQ=255)
     cgcgACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAg                                                                                                                                                  >  1:643469/1‑150 (MQ=255)
     cgcgACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAg                                                                                                                                                  >  1:3571769/1‑150 (MQ=255)
     cgcgACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAg                                                                                                                                                  >  1:3001849/1‑150 (MQ=255)
        gACAATACCGGCGAAGATACTGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGcc                                                                                                                                               <  2:4299111/150‑1 (MQ=255)
        gACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGcc                                                                                                                                               <  1:1062082/150‑1 (MQ=255)
        gACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGcc                                                                                                                                               >  2:2855815/1‑150 (MQ=255)
        gACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGcc                                                                                                                                               >  1:2707474/1‑150 (MQ=255)
          cAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCtt                                                                                                                                             <  1:2870889/150‑1 (MQ=255)
           aaTACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTg                                                                                                                                            <  2:1707585/150‑1 (MQ=255)
            aTACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGc                                                                                                                                           >  1:1518174/1‑150 (MQ=255)
                 ggCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATAcc                                                                                                                                      <  2:2601475/150‑1 (MQ=255)
                  gCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCa                                                                                                                                     <  1:2303323/150‑1 (MQ=255)
                   cGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAg                                                                                                                                    <  2:2409466/150‑1 (MQ=255)
                    gAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCCGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAgg                                                                                                                                   <  1:3380326/150‑1 (MQ=255)
                    gAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATAACCAGGCCTTGCATACCAgg                                                                                                                                   >  1:2667361/1‑150 (MQ=255)
                       gATAATGATTGAAATACCGGTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAACGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCat                                                                                                                                <  1:934803/150‑1 (MQ=255)
                          aaTGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATAtt                                                                                                                             >  1:4119976/1‑150 (MQ=255)
                           atgaatgaCAGACCGTCGCCGATACCACGTTCAGTAATCTGTTCGCCCCACCACCTCAGGCACAGGGTTCCTGGGACCAGACGTACACCAGCGGTGAAGTAGAAGGCAAACCCCGGGGTAATCCCCAGGCCTGGCATACCAGGCATCTTc                                                                                                                            <  2:4201063/150‑1 (MQ=255)
                           aTGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTc                                                                                                                            <  1:4352047/150‑1 (MQ=255)
                           aTGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTc                                                                                                                            >  1:1252653/1‑150 (MQ=255)
                           aTGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACAACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTc                                                                                                                            <  1:3511773/150‑1 (MQ=255)
                            tGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCg                                                                                                                           <  1:3765924/150‑1 (MQ=255)
                            tGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCg                                                                                                                           <  1:4065251/150‑1 (MQ=255)
                             gATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCgg                                                                                                                          >  1:2642811/1‑150 (MQ=255)
                                tGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAg                                                                                                                       >  2:1570036/1‑150 (MQ=255)
                                   aaTACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGAcc                                                                                                                    >  1:1830841/1‑150 (MQ=255)
                                   aaTACCGGTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGTATGCAAAGCCCGGGTTAATCACCAGGCCTTGAATACCAGGCATATTCGGCAGAcc                                                                                                                    <  1:1690904/150‑1 (MQ=255)
                                    aTACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCg                                                                                                                   >  1:1356239/1‑150 (MQ=255)
                                    aTACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCg                                                                                                                   <  1:1760185/150‑1 (MQ=255)
                                    aTACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCg                                                                                                                   >  2:3902501/1‑150 (MQ=255)
                                      aCCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGt                                                                                                                 >  2:1904069/1‑150 (MQ=255)
                                      aCCGTTGCCGAGACCTCGTTCAGTAATCTGGTCGCCCAACCACATCAGGACCATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGt                                                                                                                 <  2:565745/150‑1 (MQ=255)
                                        cGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAg                                                                                                               >  1:39339/1‑150 (MQ=255)
                                        cGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAg                                                                                                               >  2:1286657/1‑150 (MQ=255)
                                         gTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGc                                                                                                              >  2:745264/1‑150 (MQ=255)
                                          ttGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCa                                                                                                             <  1:1292770/150‑1 (MQ=255)
                                            gCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAAt                                                                                                           <  1:3731890/150‑1 (MQ=255)
                                             ccGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATa                                                                                                          <  2:399062/150‑1 (MQ=255)
                                             ccGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATa                                                                                                          >  2:3079984/1‑150 (MQ=255)
                                              cGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATAc                                                                                                         <  2:3595261/150‑1 (MQ=255)
                                              cGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATAc                                                                                                         >  2:1928430/1‑150 (MQ=255)
                                               gATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATAcc                                                                                                        >  2:233769/1‑150 (MQ=255)
                                                   ccTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATc                                                                                                    <  2:3683994/150‑1 (MQ=255)
                                                   ccTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATc                                                                                                    <  2:4149026/150‑1 (MQ=255)
                                                    cTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCg                                                                                                   <  1:3465437/150‑1 (MQ=255)
                                                    cTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCg                                                                                                   >  1:1484970/1‑150 (MQ=255)
                                                           aGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGaa                                                                                            >  1:3311995/1‑150 (MQ=255)
                                                             tAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAata                                                                                          <  1:4002909/150‑1 (MQ=255)
                                                                tCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTg                                                                                       <  2:4022273/150‑1 (MQ=255)
                                                                   gTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCa                                                                                    >  1:4262151/1‑150 (MQ=255)
                                                                        cccAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCAcc                                                                               >  2:3401045/1‑150 (MQ=255)
                                                                             ccACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGt                                                                          <  2:3714407/150‑1 (MQ=255)
                                                                                caTCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTAcc                                                                       <  2:1100691/150‑1 (MQ=255)
                                                                                caTCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTAcc                                                                       >  2:950628/1‑150 (MQ=255)
                                                                                caTCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTAcc                                                                       >  2:2801576/1‑150 (MQ=255)
                                                                                    aGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAg                                                                   <  2:2940947/150‑1 (MQ=255)
                                                                                    aGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAg                                                                   <  2:2829345/150‑1 (MQ=255)
                                                                                          aTGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGgtg                                                             <  2:1819092/150‑1 (MQ=255)
                                                                                           tGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGgtgt                                                            <  2:3263925/150‑1 (MQ=255)
                                                                                                  tgtgACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCt                                                     <  2:2216057/150‑1 (MQ=255)
                                                                                                  tgtgACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCt                                                     >  2:1341520/1‑150 (MQ=255)
                                                                                                     gACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGAt                                                  <  2:3468884/150‑1 (MQ=255)
                                                                                                        cAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCtt                                               <  2:2190615/150‑1 (MQ=255)
                                                                                                         aGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTa                                              <  2:108156/150‑1 (MQ=255)
                                                                                                            cTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTacga                                           <  1:324854/150‑1 (MQ=255)
                                                                                                             ttACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTacgac                                          <  1:1554142/150‑1 (MQ=255)
                                                                                                              tACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTacgacg                                         <  1:1024182/150‑1 (MQ=255)
                                                                                                              tACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTacgacg                                         >  1:991595/1‑150 (MQ=255)
                                                                                                              tACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTacgacg                                         >  1:3639932/1‑150 (MQ=255)
                                                                                                                  acaGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCa                                     <  2:4319032/150‑1 (MQ=255)
                                                                                                                    aGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGa                                   >  1:1999389/1‑150 (MQ=255)
                                                                                                                      cGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACt                                 >  1:2968603/1‑150 (MQ=255)
                                                                                                                      cGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACt                                 >  2:1139114/1‑150 (MQ=255)
                                                                                                                      cGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACt                                 >  1:795720/1‑150 (MQ=255)
                                                                                                                       ggTGAAGTAGAATGCAAAGCCCGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTc                                <  1:2086575/150‑1 (MQ=255)
                                                                                                                          gAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTcccc                             <  2:1968362/150‑1 (MQ=255)
                                                                                                                           aaGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCct                            >  1:639252/1‑150 (MQ=255)
                                                                                                                            aGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCctt                           >  1:1713755/1‑150 (MQ=255)
                                                                                                                              tAGAATGCAACGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCcttct                         <  1:1100525/150‑1 (MQ=255)
                                                                                                                              tAGAATGCAAAGCCCGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCcttct                         >  2:2604465/1‑150 (MQ=255)
                                                                                                                                gAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCttcttt                       >  1:3166716/1‑150 (MQ=255)
                                                                                                                                   tGCAAAGCCCGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTtctttctt                    <  2:505051/150‑1 (MQ=255)
                                                                                                                                   tGCAAAGCCCGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTtctttctt                    >  1:2341864/1‑150 (MQ=255)
                                                                                                                                     cAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTCCTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTaa                  >  2:3830984/1‑150 (MQ=255)
                                                                                                                                      aaaGCCCGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAAt                 <  1:3063558/150‑1 (MQ=255)
                                                                                                                                      aaaGCCCGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAAt                 <  1:2714739/150‑1 (MQ=255)
                                                                                                                                         gCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTc              <  1:1941642/150‑1 (MQ=255)
                                                                                                                                          cccGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCt             <  1:1772190/150‑1 (MQ=255)
                                                                                                                                          cccGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCt             >  1:559840/1‑150 (MQ=255)
                                                                                                                                          cccGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCt             <  1:386770/150‑1 (MQ=255)
                                                                                                                                           ccGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTg            >  2:411392/1‑150 (MQ=255)
                                                                                                                                           ccGGGTTAATAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTg            >  2:411342/1‑150 (MQ=255)
                                                                                                                                            cGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGc           <  2:3001849/150‑1 (MQ=255)
                                                                                                                                                 taataaCCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACg      >  1:2485764/1‑150 (MQ=255)
                                                                                                                                                  aaTAACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGt     >  1:508459/1‑150 (MQ=255)
                                                                                                                                                     cACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTgg  <  2:3124717/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGG  >  CP009273/3436861‑3437159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A