Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,440,444 G→T 100% S118S (TCC→TCA)  rplE ← 50S ribosomal subunit protein L5

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,440,4440GT95.1% 898.3 / 31.1 285S118S (TCC→TCA) rplE50S ribosomal subunit protein L5
Reads supporting (aligned to +/- strand):  ref base G (6/8);  new base T (140/131);  total (146/139)
Fisher's exact test for biased strand distribution p-value = 5.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 285 total aligned reads displayed.

GCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTTGCGTGC  >  CP009273/3440297‑3440590
                                                                                                                                                   |                                                                                                                                                  
   gCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAg                                                                                                                                               <  2:1066344/150‑1 (MQ=255)
       tcttcGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCa                                                                                                                                           <  1:1374666/150‑1 (MQ=255)
                gATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCa                                                                                                                                  >  2:878761/1‑150 (MQ=255)
                  tttCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACg                                                                                                                                <  1:883229/150‑1 (MQ=255)
                     cGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACTGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGAt                                                                                                                             <  1:3889977/150‑1 (MQ=255)
                     cGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGAt                                                                                                                             <  1:1012852/150‑1 (MQ=255)
                     cGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGAt                                                                                                                             >  1:771860/1‑150 (MQ=255)
                      gCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATa                                                                                                                            <  2:1163623/150‑1 (MQ=255)
                       cAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATAc                                                                                                                           >  2:257272/1‑150 (MQ=255)
                        agtagtGGTACTGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACg                                                                                                                          >  2:3134598/1‑150 (MQ=255)
                         gtagtGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGa                                                                                                                         <  1:2476861/150‑1 (MQ=255)
                           agtGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCTCGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAgg                                                                                                                       >  2:1907537/1‑150 (MQ=255)
                           agtGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAgg                                                                                                                       <  1:536069/150‑1 (MQ=255)
                           agtGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAgg                                                                                                                       >  2:2022392/1‑150 (MQ=255)
                            gtGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGt                                                                                                                      >  2:2512426/1‑150 (MQ=255)
                            gtGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGt                                                                                                                      >  2:2124563/1‑150 (MQ=255)
                             tggtaatggtaaTATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTa                                                                                                                     >  1:4234160/1‑150 (MQ=255)
                             tggtaatggtaaTATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTa                                                                                                                     >  1:4233959/1‑150 (MQ=255)
                              ggtaatggtaatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTAc                                                                                                                    >  2:2252839/1‑150 (MQ=255)
                                 aatggtaatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGc                                                                                                                 <  1:752112/150‑1 (MQ=255)
                                  atggtaatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCa                                                                                                                >  1:3779523/1‑150 (MQ=255)
                                  atggtaatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCa                                                                                                                >  2:375226/1‑150 (MQ=255)
                                  atggtaatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCa                                                                                                                >  2:374684/1‑150 (MQ=255)
                                   tggtaatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGcta                                                                                                               >  2:314856/1‑148 (MQ=255)
                                   tggtaatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCaa                                                                                                               >  1:1076095/1‑150 (MQ=255)
                                    ggtaatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAAt                                                                                                              <  2:2874271/150‑1 (MQ=255)
                                     gtaatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATa                                                                                                             >  1:2096504/1‑150 (MQ=255)
                                     gtaatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATa                                                                                                             >  1:1002997/1‑150 (MQ=255)
                                     gtaatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATa                                                                                                             >  1:3280074/1‑150 (MQ=255)
                                        atatCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTg                                                                                                          >  2:1598636/1‑150 (MQ=255)
                                          atCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGAt                                                                                                        <  2:541148/150‑1 (MQ=255)
                                           tCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATc                                                                                                       <  1:2532080/150‑1 (MQ=255)
                                            ccAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCa                                                                                                      <  1:3537398/150‑1 (MQ=255)
                                                 ccACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGgcgc                                                                                                 >  2:4175852/1‑150 (MQ=255)
                                                  cACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCt                                                                                                <  2:1133875/150‑1 (MQ=255)
                                                     gAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCaa                                                                                             >  2:244989/1‑150 (MQ=255)
                                                      aaCGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCaaa                                                                                            <  2:2057389/150‑1 (MQ=255)
                                                         gcgGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGaa                                                                                         <  2:3331108/150‑1 (MQ=255)
                                                         gcgGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGaa                                                                                         <  2:3657951/150‑1 (MQ=255)
                                                         gcgGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGaa                                                                                         <  2:1391573/150‑1 (MQ=255)
                                                          cgGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAAc                                                                                        >  2:3193760/1‑150 (MQ=255)
                                                          cgGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAAc                                                                                        <  2:2837444/150‑1 (MQ=255)
                                                          cgGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAAc                                                                                        >  2:1690862/1‑150 (MQ=255)
                                                               gACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTAAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAATAACTCCca                                                                                   >  1:4077076/1‑150 (MQ=255)
                                                                    tATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGc                                                                              <  2:2649755/150‑1 (MQ=255)
                                                                    tATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGc                                                                              <  2:1737246/150‑1 (MQ=255)
                                                                     aTCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCg                                                                             <  1:142692/150‑1 (MQ=255)
                                                                        gTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACATGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTc                                                                          <  2:2395363/150‑1 (MQ=255)
                                                                           gTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGcc                                                                       <  2:470022/150‑1 (MQ=255)
                                                                           gTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGcc                                                                       >  2:1610186/1‑150 (MQ=255)
                                                                            tCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCa                                                                      <  2:1469244/150‑1 (MQ=255)
                                                                             cGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCAc                                                                     <  2:1504372/150‑1 (MQ=255)
                                                                             cGATTTCTGGGAAGATGATCAGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGAAACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCAc                                                                     >  2:366857/1‑150 (MQ=255)
                                                                                tttCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCa                                                                  <  2:663484/150‑1 (MQ=255)
                                                                                       gAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTAc                                                           >  1:2018418/1‑150 (MQ=255)
                                                                                              atCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAg                                                    <  1:3635513/150‑1 (MQ=255)
                                                                                                cTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGcc                                                  <  2:1439853/150‑1 (MQ=255)
                                                                                                 tGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCg                                                 >  1:633715/1‑150 (MQ=255)
                                                                                                 tGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGACTTACTTTACAGCCg                                                 >  1:2913430/1‑150 (MQ=255)
                                                                                                    tCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATc                                              <  2:3828461/150‑1 (MQ=255)
                                                                                                         gACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATa                                         <  1:1535124/150‑1 (MQ=255)
                                                                                                           cacCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGc                                       >  2:133015/1‑150 (MQ=255)
                                                                                                           cacCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGc                                       >  1:2719023/1‑150 (MQ=255)
                                                                                                           cacCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGc                                       >  1:3899716/1‑150 (MQ=255)
                                                                                                            acCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGcc                                      >  1:3567226/1‑150 (MQ=255)
                                                                                                            acCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGcc                                      >  1:1740983/1‑150 (MQ=255)
                                                                                                            acCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGcc                                      >  2:181541/1‑150 (MQ=255)
                                                                                                              ccATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCt                                    >  2:859342/1‑150 (MQ=255)
                                                                                                              ccATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCt                                    >  1:1157731/1‑150 (MQ=255)
                                                                                                              ccATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCt                                    <  1:1860057/150‑1 (MQ=255)
                                                                                                                 tGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGAc                                 <  2:3108330/150‑1 (MQ=255)
                                                                                                                  gCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACg                                >  2:4070196/1‑150 (MQ=255)
                                                                                                                  gCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACg                                >  2:814142/1‑150 (MQ=255)
                                                                                                                    tGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATTGGATAGCCCTGACGGa                              >  1:936405/1‑150 (MQ=255)
                                                                                                                    tGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGa                              >  1:1992407/1‑150 (MQ=255)
                                                                                                                    tGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGa                              >  1:1224729/1‑150 (MQ=255)
                                                                                                                     gTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGAt                             <  1:3233447/150‑1 (MQ=255)
                                                                                                                     gTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGAt                             <  1:4412505/150‑1 (MQ=255)
                                                                                                                     gTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGAt                             <  1:580567/150‑1 (MQ=255)
                                                                                                                     gTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGAt                             <  2:131328/150‑1 (MQ=255)
                                                                                                                        gTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGAtttt                          <  2:42488/150‑1 (MQ=255)
                                                                                                                         ttACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTg                         <  2:1916605/150‑1 (MQ=255)
                                                                                                                           aCCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGaa                       <  1:2158838/150‑1 (MQ=255)
                                                                                                                            ccACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAg                      <  2:4481636/150‑1 (MQ=255)
                                                                                                                               cGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCt                   <  1:878761/150‑1 (MQ=255)
                                                                                                                                 aCCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAGCTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGc                 <  2:2686123/150‑1 (MQ=255)
                                                                                                                                    gTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAAc              <  1:1444126/150‑1 (MQ=255)
                                                                                                                                    gTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAAc              <  1:2537485/150‑1 (MQ=255)
                                                                                                                                     tCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACa             <  1:3755308/150‑1 (MQ=255)
                                                                                                                                      cGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAg            <  1:2345215/150‑1 (MQ=255)
                                                                                                                                       gAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGa           >  1:2070001/1‑150 (MQ=255)
                                                                                                                                       gAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGa           >  1:261667/1‑150 (MQ=255)
                                                                                                                                       gAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGa           >  1:4314590/1‑150 (MQ=255)
                                                                                                                                          aGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGAttt        <  1:1657753/150‑1 (MQ=255)
                                                                                                                                          aGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGAttt        <  1:374684/150‑1 (MQ=255)
                                                                                                                                          aGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGAttt        <  2:959400/150‑1 (MQ=255)
                                                                                                                                          aGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGAttt        <  2:3237035/150‑1 (MQ=255)
                                                                                                                                           gACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTTg       <  2:3539944/150‑1 (MQ=255)
                                                                                                                                            aCTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTTGc      <  1:2252839/150‑1 (MQ=255)
                                                                                                                                             cTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTTGCg     <  1:3170687/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTTGCGTGC  >  CP009273/3440297‑3440590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTTGCGTGCTTT  >  CP009273/3440296‑3440593
                                                                                                                                                    |                                                                                                                                                     
CGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTG                                                                                                                                                      >  SRR19973076.1502864/1‑150 (MQ=60)
 GCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGTTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGA                                                                                                                                                     <  SRR19973076.3001911/150‑1 (MQ=60)
 GCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGA                                                                                                                                                     <  SRR19973076.3459962/150‑1 (MQ=60)
  CGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGAC                                                                                                                                                    <  SRR19973076.301946/150‑1 (MQ=60)
  CGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGAC                                                                                                                                                    >  SRR19973076.1780969/1‑150 (MQ=60)
     CCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGG                                                                                                                                                 >  SRR19973076.2690023/1‑150 (MQ=60)
     CCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGG                                                                                                                                                 >  SRR19973076.2169265/1‑150 (MQ=60)
       TTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCC                                                                                                                                               <  SRR19973076.1997555/150‑1 (MQ=60)
        TCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCA                                                                                                                                              <  SRR19973076.1376686/150‑1 (MQ=60)
             GTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAA                                                                                                                                         >  SRR19973076.1127358/1‑150 (MQ=60)
              TCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAG                                                                                                                                        >  SRR19973076.3525097/1‑150 (MQ=60)
                AGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTC                                                                                                                                      >  SRR19973076.2060387/1‑150 (MQ=60)
                   TTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACG                                                                                                                                   <  SRR19973076.884128/150‑1 (MQ=60)
                   TTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACG                                                                                                                                   <  SRR19973076.884531/150‑1 (MQ=60)
                   TTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACG                                                                                                                                   <  SRR19973076.884538/150‑1 (MQ=60)
                      CGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACTGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGAT                                                                                                                                <  SRR19973076.3895724/150‑1 (MQ=60)
                      CGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGAT                                                                                                                                >  SRR19973076.772988/1‑150 (MQ=60)
                      CGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGAT                                                                                                                                <  SRR19973076.1014353/150‑1 (MQ=60)
                      CGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGAT                                                                                                                                <  SRR19973076.1014358/150‑1 (MQ=60)
                       GCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATA                                                                                                                               <  SRR19973076.2148505/150‑1 (MQ=60)
                          GTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGA                                                                                                                            <  SRR19973076.2480460/150‑1 (MQ=60)
                            AGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGG                                                                                                                          <  SRR19973076.536920/150‑1 (MQ=60)
                              TGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTA                                                                                                                        >  SRR19973076.4240415/1‑150 (MQ=60)
                              TGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTA                                                                                                                        >  SRR19973076.4240404/1‑150 (MQ=60)
                              TGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTA                                                                                                                        >  SRR19973076.1083194/1‑150 (MQ=60)
                              TGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTA                                                                                                                        >  SRR19973076.4240284/1‑150 (MQ=60)
                              TGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTA                                                                                                                        >  SRR19973076.4240214/1‑150 (MQ=60)
                               GGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTAC                                                                                                                       <  SRR19973076.1433141/150‑1 (MQ=60)
                                  AATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGC                                                                                                                    <  SRR19973076.753221/150‑1 (MQ=60)
                                  AATGGTAATATCCAAACCACGAACGCGGTCGACTGTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGC                                                                                                                    <  SRR19973076.164988/150‑1 (MQ=60)
                                   ATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCA                                                                                                                   >  SRR19973076.3785064/1‑150 (MQ=60)
                                   ATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCA                                                                                                                   >  SRR19973076.3597782/1‑150 (MQ=60)
                                   ATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCA                                                                                                                   >  SRR19973076.4401770/1‑150 (MQ=60)
                                   ATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCA                                                                                                                   >  SRR19973076.3597737/1‑150 (MQ=60)
                                    TGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAA                                                                                                                  >  SRR19973076.1077699/1‑150 (MQ=60)
                                      GTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATA                                                                                                                >  SRR19973076.2099549/1‑150 (MQ=60)
                                      GTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATA                                                                                                                >  SRR19973076.2099548/1‑150 (MQ=60)
                                      GTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATA                                                                                                                >  SRR19973076.3284834/1‑150 (MQ=60)
                                      GTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATA                                                                                                                >  SRR19973076.2099486/1‑150 (MQ=60)
                                      GTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATA                                                                                                                >  SRR19973076.1004485/1‑150 (MQ=60)
                                         ATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTG                                                                                                             >  SRR19973076.3247773/1‑150 (MQ=60)
                                            TCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATC                                                                                                          <  SRR19973076.571852/150‑1 (MQ=60)
                                            TCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATC                                                                                                          <  SRR19973076.2535764/150‑1 (MQ=60)
                                             CCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCA                                                                                                         <  SRR19973076.3542532/150‑1 (MQ=60)
                                               AACCCACGAACGCGGTCGACTTTCTCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGG                                                                                                       <  SRR19973076.3806862/150‑1 (MQ=60)
                                                AACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGC                                                                                                      <  SRR19973076.502645/150‑1 (MQ=60)
                                                   CACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCT                                                                                                   <  SRR19973076.139332/150‑1 (MQ=60)
                                                   CACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCT                                                                                                   <  SRR19973076.434853/150‑1 (MQ=60)
                                                       AACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACTACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCATGCGCTCAAA                                                                                               >  SRR19973076.2536037/1‑150 (MQ=60)
                                                       AACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAA                                                                                               >  SRR19973076.4103253/1‑150 (MQ=60)
                                                              TCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCC                                                                                        >  SRR19973076.3568281/1‑150 (MQ=60)
                                                                GACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCA                                                                                      >  SRR19973076.4282174/1‑150 (MQ=60)
                                                                GACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCA                                                                                      >  SRR19973076.4282368/1‑150 (MQ=60)
                                                                GACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTAAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAATAACTCCCA                                                                                      >  SRR19973076.4083099/1‑150 (MQ=60)
                                                                    TTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCAAGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATG                                                                                  <  SRR19973076.3773704/150‑1 (MQ=60)
                                                                      ATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCG                                                                                <  SRR19973076.142870/150‑1 (MQ=60)
                                                                      ATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCG                                                                                <  SRR19973076.142942/150‑1 (MQ=60)
                                                                      ATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACCGACACCCCTGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACACGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCG                                                                                >  SRR19973076.247557/1‑150 (MQ=60)
                                                                          TAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCG                                                                            >  SRR19973076.2548545/1‑150 (MQ=60)
                                                                                  TTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAG                                                                    <  SRR19973076.65252/150‑1 (MQ=60)
                                                                                     TGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGT                                                                 >  SRR19973076.1515687/1‑150 (MQ=60)
                                                                                        GAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTAC                                                              >  SRR19973076.2021374/1‑150 (MQ=60)
                                                                                            ATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTA                                                          >  SRR19973076.4191002/1‑150 (MQ=60)
                                                                                            ATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTA                                                          >  SRR19973076.200721/1‑150 (MQ=60)
                                                                                             TGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTAC                                                         >  SRR19973076.602748/1‑150 (MQ=60)
                                                                                               ATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAG                                                       <  SRR19973076.3640798/150‑1 (MQ=60)
                                                                                                 CTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCC                                                     >  SRR19973076.966522/1‑150 (MQ=60)
                                                                                                 CTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCC                                                     >  SRR19973076.1541853/1‑150 (MQ=60)
                                                                                                  TGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCG                                                    >  SRR19973076.634682/1‑150 (MQ=60)
                                                                                                  TGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCG                                                    >  SRR19973076.770480/1‑150 (MQ=60)
                                                                                                  TGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCG                                                    >  SRR19973076.770507/1‑150 (MQ=60)
                                                                                                  TGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGACTTACTTTACAGCCG                                                    >  SRR19973076.2917668/1‑150 (MQ=60)
                                                                                                    CTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGAT                                                  >  SRR19973076.4065289/1‑150 (MQ=60)
                                                                                                       ACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGG                                               <  SRR19973076.2617943/150‑1 (MQ=60)
                                                                                                         GGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGAT                                             <  SRR19973076.2082442/150‑1 (MQ=60)
                                                                                                          GACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATA                                            <  SRR19973076.1537374/150‑1 (MQ=60)
                                                                                                          GACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATA                                            <  SRR19973076.1537368/150‑1 (MQ=60)
                                                                                                           ACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAG                                           <  SRR19973076.402861/150‑1 (MQ=60)
                                                                                                            CACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGC                                          >  SRR19973076.2722977/1‑150 (MQ=60)
                                                                                                            CACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGC                                          >  SRR19973076.3905342/1‑150 (MQ=60)
                                                                                                            CACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGC                                          >  SRR19973076.3905464/1‑150 (MQ=60)
                                                                                                            CACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGC                                          >  SRR19973076.3905481/1‑150 (MQ=60)
                                                                                                            CACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGC                                          >  SRR19973076.2722969/1‑150 (MQ=60)
                                                                                                             ACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCC                                         >  SRR19973076.1743494/1‑150 (MQ=60)
                                                                                                             ACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCC                                         >  SRR19973076.1743503/1‑150 (MQ=60)
                                                                                                             ACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCC                                         >  SRR19973076.3572409/1‑150 (MQ=60)
                                                                                                               CCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCT                                       <  SRR19973076.1056169/150‑1 (MQ=60)
                                                                                                               CCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCT                                       >  SRR19973076.1159449/1‑150 (MQ=60)
                                                                                                               CCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCT                                       <  SRR19973076.1862115/150‑1 (MQ=60)
                                                                                                               CCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCT                                       <  SRR19973076.1862747/150‑1 (MQ=60)
                                                                                                                     TGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATTGGATAGCCCTGACGGA                                 >  SRR19973076.937796/1‑150 (MQ=60)
                                                                                                                     TGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGA                                 >  SRR19973076.1226589/1‑150 (MQ=60)
                                                                                                                     TGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGA                                 >  SRR19973076.1995301/1‑150 (MQ=60)
                                                                                                                     TGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGA                                 >  SRR19973076.1226739/1‑150 (MQ=60)
                                                                                                                     TGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGA                                 >  SRR19973076.1226540/1‑150 (MQ=60)
                                                                                                                     TGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGA                                 >  SRR19973076.1226537/1‑150 (MQ=60)
                                                                                                                     TGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGA                                 >  SRR19973076.937700/1‑150 (MQ=60)
                                                                                                                     TGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGA                                 >  SRR19973076.1226541/1‑150 (MQ=60)
                                                                                                                      GTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGAT                                <  SRR19973076.581459/150‑1 (MQ=60)
                                                                                                                      GTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGAT                                <  SRR19973076.4419050/150‑1 (MQ=60)
                                                                                                                      GTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGAT                                <  SRR19973076.3238148/150‑1 (MQ=60)
                                                                                                                      GTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGAT                                >  SRR19973076.1072055/1‑150 (MQ=60)
                                                                                                                      GTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGAT                                <  SRR19973076.3728612/150‑1 (MQ=60)
                                                                                                                      GTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGAT                                <  SRR19973076.581507/150‑1 (MQ=60)
                                                                                                                      GTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGAT                                <  SRR19973076.581462/150‑1 (MQ=60)
                                                                                                                      GTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGAT                                >  SRR19973076.2357469/1‑150 (MQ=60)
                                                                                                                       TAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATT                               >  SRR19973076.1866545/1‑150 (MQ=60)
                                                                                                                          TTACCACGCCCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTG                            <  SRR19973076.3203605/150‑1 (MQ=60)
                                                                                                                          TTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTG                            <  SRR19973076.3545102/150‑1 (MQ=60)
                                                                                                                          TTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTG                            <  SRR19973076.3203662/150‑1 (MQ=60)
                                                                                                                            ACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAA                          >  SRR19973076.284289/1‑150 (MQ=60)
                                                                                                                            ACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAA                          >  SRR19973076.284251/1‑150 (MQ=60)
                                                                                                                            ACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAA                          <  SRR19973076.2161963/150‑1 (MQ=60)
                                                                                                                            ACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAA                          <  SRR19973076.2167602/150‑1 (MQ=60)
                                                                                                                                CGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCGGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCT                      <  SRR19973076.879964/150‑1 (MQ=60)
                                                                                                                                CGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCT                      >  SRR19973076.2272126/1‑150 (MQ=60)
                                                                                                                                CGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCT                      >  SRR19973076.2273261/1‑150 (MQ=60)
                                                                                                                                CGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCT                      <  SRR19973076.880058/150‑1 (MQ=60)
                                                                                                                                CGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCT                      >  SRR19973076.3645250/1‑150 (MQ=60)
                                                                                                                                     GTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAAC                 <  SRR19973076.1446243/150‑1 (MQ=60)
                                                                                                                                     GTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAAC                 <  SRR19973076.2541177/150‑1 (MQ=60)
                                                                                                                                     GTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAAC                 <  SRR19973076.1709081/150‑1 (MQ=60)
                                                                                                                                      TCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACA                <  SRR19973076.1872861/150‑1 (MQ=60)
                                                                                                                                      TCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACA                <  SRR19973076.3760804/150‑1 (MQ=60)
                                                                                                                                      TCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACA                <  SRR19973076.3781604/150‑1 (MQ=60)
                                                                                                                                       CGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAG               <  SRR19973076.723680/150‑1 (MQ=60)
                                                                                                                                       CGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAG               <  SRR19973076.2348588/150‑1 (MQ=60)
                                                                                                                                        GAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGA              >  SRR19973076.2073017/1‑150 (MQ=60)
                                                                                                                                        GAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGA              >  SRR19973076.4320961/1‑150 (MQ=60)
                                                                                                                                        GAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGA              >  SRR19973076.262101/1‑150 (MQ=60)
                                                                                                                                          AAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATT            >  SRR19973076.2488880/1‑150 (MQ=60)
                                                                                                                                          AAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATT            >  SRR19973076.2488865/1‑150 (MQ=60)
                                                                                                                                           AGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTT           <  SRR19973076.375487/150‑1 (MQ=60)
                                                                                                                                           AGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTT           <  SRR19973076.375270/150‑1 (MQ=60)
                                                                                                                                           AGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTT           <  SRR19973076.1660174/150‑1 (MQ=60)
                                                                                                                                           AGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTT           <  SRR19973076.375813/150‑1 (MQ=60)
                                                                                                                                             ACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTTGC         <  SRR19973076.2256072/150‑1 (MQ=60)
                                                                                                                                              CTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTTGCG        <  SRR19973076.3175282/150‑1 (MQ=60)
                                                                                                                                                TAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTTGCGTG      <  SRR19973076.863449/150‑1 (MQ=60)
                                                                                                                                                    TGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTTGCGTGCTTT  <  SRR19973076.3657076/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
CGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTTGCGTGCTTT  >  CP009273/3440296‑3440593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑