Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,440,444 G→T 100% S118S (TCC→TCA)  rplE ← 50S ribosomal subunit protein L5

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,440,4440GT95.4% 830.2 / 27.0 258S118S (TCC→TCA) rplE50S ribosomal subunit protein L5
Reads supporting (aligned to +/- strand):  ref base G (6/6);  new base T (133/113);  total (139/119)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 258 total aligned reads displayed.

CGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTTGCGTG  >  CP009273/3440298‑3440589
                                                                                                                                                  |                                                                                                                                                 
 ggCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCCCGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACa                                                                                                                                               <  2:1007276/150‑1 (MQ=255)
 ggCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACa                                                                                                                                               <  1:4253033/150‑1 (MQ=255)
 ggCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACa                                                                                                                                               >  2:4033022/1‑150 (MQ=255)
 ggCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACa                                                                                                                                               <  2:4052934/150‑1 (MQ=255)
 ggCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACa                                                                                                                                               <  2:4270087/150‑1 (MQ=255)
  gCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAg                                                                                                                                              <  1:3702208/150‑1 (MQ=255)
    cttcttCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGc                                                                                                                                            <  1:2229838/150‑1 (MQ=255)
         tcGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACgg                                                                                                                                       >  1:1091440/1‑150 (MQ=255)
            tCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAg                                                                                                                                    >  2:3225705/1‑150 (MQ=255)
             cAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGt                                                                                                                                   >  2:4414694/1‑150 (MQ=255)
             cAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGATACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGt                                                                                                                                   >  2:3669102/1‑150 (MQ=255)
              aGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTc                                                                                                                                  <  2:3864792/150‑1 (MQ=255)
              aGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTc                                                                                                                                  >  2:1505652/1‑150 (MQ=255)
                aTTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCAc                                                                                                                                >  1:3183370/1‑150 (MQ=255)
                  ttCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACgg                                                                                                                              >  1:769974/1‑150 (MQ=255)
                     gCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATa                                                                                                                           >  1:542283/1‑150 (MQ=255)
                     gCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATa                                                                                                                           >  2:1832596/1‑150 (MQ=255)
                      cAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATAc                                                                                                                          <  1:687553/150‑1 (MQ=255)
                       agtagtGGTAATAGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACACATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATaag                                                                                                                         >  1:257874/1‑148 (MQ=255)
                        gtagtGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGa                                                                                                                        >  1:3600275/1‑150 (MQ=255)
                         tagtGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAg                                                                                                                       >  2:4519033/1‑150 (MQ=255)
                            tggtaatggtaaTATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTa                                                                                                                    <  2:1968848/150‑1 (MQ=255)
                            tggtaatggtaaTATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTa                                                                                                                    <  2:2488902/150‑1 (MQ=255)
                            tggtaatggtaaTATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTa                                                                                                                    >  1:2467616/1‑150 (MQ=255)
                            tggtaatggtaaTATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTa                                                                                                                    >  1:1496348/1‑150 (MQ=255)
                              gtaatggtaatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACa                                                                                                                  >  1:1187146/1‑150 (MQ=255)
                                 atggtaatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACATGCCACGGAAGTCACGGATACGAGGTACAGCa                                                                                                               >  2:231529/1‑150 (MQ=255)
                                   ggtaatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAAt                                                                                                             <  1:3068939/150‑1 (MQ=255)
                                    gtaatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATa                                                                                                            >  1:1352521/1‑150 (MQ=255)
                                     taatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAg                                                                                                           <  2:2064639/150‑1 (MQ=255)
                                     taatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAg                                                                                                           >  2:1228356/1‑150 (MQ=255)
                                         atCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGAt                                                                                                       >  2:3403916/1‑150 (MQ=255)
                                              aaCCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGgc                                                                                                  >  1:2291426/1‑150 (MQ=255)
                                                 cACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCt                                                                                               <  1:1327400/150‑1 (MQ=255)
                                                      aCGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAg                                                                                          <  2:1087532/150‑1 (MQ=255)
                                                       cgcgGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGa                                                                                         <  1:3997668/150‑1 (MQ=255)
                                                       cgcgGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGa                                                                                         <  1:847017/150‑1 (MQ=255)
                                                        gcgGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGaa                                                                                        <  2:2140039/150‑1 (MQ=255)
                                                          ggTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACt                                                                                      >  2:1562366/1‑150 (MQ=255)
                                                           gTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTc                                                                                     >  2:133092/1‑150 (MQ=255)
                                                            tCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTcc                                                                                    <  2:3136000/150‑1 (MQ=255)
                                                            tCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTcc                                                                                    >  1:4484097/1‑150 (MQ=255)
                                                             cGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTccc                                                                                   <  1:4171540/150‑1 (MQ=255)
                                                             cGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTccc                                                                                   <  1:1305902/150‑1 (MQ=255)
                                                              gACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCca                                                                                  >  2:881095/1‑150 (MQ=255)
                                                                 tttATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACAt                                                                               >  2:3324260/1‑150 (MQ=255)
                                                                 tttATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACAt                                                                               <  2:4584111/150‑1 (MQ=255)
                                                                  ttATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATg                                                                              <  2:1937920/150‑1 (MQ=255)
                                                                  ttATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATg                                                                              <  2:1209109/150‑1 (MQ=255)
                                                                   tATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGc                                                                             <  1:2875290/150‑1 (MQ=255)
                                                                    aTCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCg                                                                            <  2:2905985/150‑1 (MQ=255)
                                                                    aTCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCg                                                                            >  1:1790589/1‑150 (MQ=255)
                                                                        tAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCg                                                                        <  2:2804488/150‑1 (MQ=255)
                                                                         aGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGc                                                                       >  1:3413653/1‑150 (MQ=255)
                                                                            cGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCAc                                                                    >  2:4320704/1‑150 (MQ=255)
                                                                            cGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCAc                                                                    <  2:990272/150‑1 (MQ=255)
                                                                             gATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACg                                                                   >  1:4343165/1‑150 (MQ=255)
                                                                               tttCTTGGAAGATGAACTGCTCACGGACACCCATGCAGTAGTTACAACGACCGACTAAAGACTTAGCTGACAGTCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCACAAAGAACTCCCACATGCGTTCGCCACGCa                                                                 >  2:2601566/1‑150 (MQ=255)
                                                                               tttCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCa                                                                 <  2:949009/150‑1 (MQ=255)
                                                                                  cTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCagag                                                              <  1:2792768/150‑1 (MQ=255)
                                                                                      gAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATCCGAGGTACAGCAATAGTGATCAGGCGCTCCATGAACTCCCACATGCGTTCGCCACGCAGAGTTag                                                          >  2:3558481/1‑149 (MQ=255)
                                                                                      gAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTAc                                                          >  2:1361702/1‑150 (MQ=255)
                                                                                        aGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACtt                                                        >  1:3651551/1‑150 (MQ=255)
                                                                                         gatgatCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACttt                                                       >  1:1534226/1‑150 (MQ=255)
                                                                                         gatgatCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACttt                                                       >  1:32621/1‑150 (MQ=255)
                                                                                          atgatCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACATTa                                                      >  2:83451/1‑150 (MQ=255)
                                                                                                 gCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGa                                               >  1:3538356/1‑150 (MQ=255)
                                                                                                   tCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATc                                             >  2:2994660/1‑150 (MQ=255)
                                                                                                      cGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGa                                          >  1:3239185/1‑150 (MQ=255)
                                                                                                      cGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGa                                          <  1:1395386/150‑1 (MQ=255)
                                                                                                       ggACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGAt                                         >  1:2680812/1‑150 (MQ=255)
                                                                                                        gACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATa                                        <  2:616776/150‑1 (MQ=255)
                                                                                                           acCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGcc                                     <  2:3088693/150‑1 (MQ=255)
                                                                                                           acCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGcc                                     <  2:3274955/150‑1 (MQ=255)
                                                                                                             ccATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCt                                   <  1:621795/150‑1 (MQ=255)
                                                                                                                   tGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGa                             <  1:1654826/150‑1 (MQ=255)
                                                                                                                    gTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGAt                            <  1:303649/150‑1 (MQ=255)
                                                                                                                     tAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGAtt                           <  1:4066717/150‑1 (MQ=255)
                                                                                                                     tAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGAtt                           <  1:2013820/150‑1 (MQ=255)
                                                                                                                      aGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGAttt                          <  2:1735660/150‑1 (MQ=255)
                                                                                                                      aGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGAttt                          <  2:2835442/150‑1 (MQ=255)
                                                                                                                          aCCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGaa                      <  2:2241945/150‑1 (MQ=255)
                                                                                                                            cACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGc                    >  1:4478674/1‑150 (MQ=255)
                                                                                                                              cGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCt                  <  2:1791375/150‑1 (MQ=255)
                                                                                                                              cGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCt                  <  2:542794/150‑1 (MQ=255)
                                                                                                                              cGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCt                  <  2:1150867/150‑1 (MQ=255)
                                                                                                                                aCCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGc                >  2:4199185/1‑150 (MQ=255)
                                                                                                                                aCCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGc                >  2:1427/1‑150 (MQ=255)
                                                                                                                                   gTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAAc             >  1:2900028/1‑150 (MQ=255)
                                                                                                                                     cGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAg           <  1:3352834/150‑1 (MQ=255)
                                                                                                                                     cGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAg           <  1:3751454/150‑1 (MQ=255)
                                                                                                                                       aaaGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGAt         >  1:91863/1‑150 (MQ=255)
                                                                                                                                       aaaGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGAt         >  1:882726/1‑150 (MQ=255)
                                                                                                                                       aaaGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGAt         >  1:1728770/1‑150 (MQ=255)
                                                                                                                                        aaGACTTATCTGACATGCCACTGAAGTCACGGATATGATGTACATCTATAGTTATCAGGCGCTCAATTAACTCACACATGCGTTAGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGAAGGATTTTGAAGCCTGCAACAGAtt        <  2:506504/150‑1 (MQ=255)
                                                                                                                                        aaGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGAtt        <  1:2588591/150‑1 (MQ=255)
                                                                                                                                         aGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGAttt       <  1:2521552/150‑1 (MQ=255)
                                                                                                                                          gACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTTg      <  2:827959/150‑1 (MQ=255)
                                                                                                                                           aCTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTTGc     >  1:2450937/1‑150 (MQ=255)
                                                                                                                                              tAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTTGCGTg  >  2:4406794/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTTGCGTG  >  CP009273/3440298‑3440589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTTGCGTGCTTT  >  CP009273/3440295‑3440593
                                                                                                                                                     |                                                                                                                                                     
GCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCT                                                                                                                                                       <  SRR19973073.1502542/150‑1 (MQ=60)
    GGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACA                                                                                                                                                   <  SRR19973073.4261446/150‑1 (MQ=60)
     GCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAG                                                                                                                                                  <  SRR19973073.3709643/150‑1 (MQ=60)
       CTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGC                                                                                                                                                <  SRR19973073.2234447/150‑1 (MQ=60)
          ATTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCAC                                                                                                                                             <  SRR19973073.982423/150‑1 (MQ=60)
           TTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACG                                                                                                                                            >  SRR19973073.890438/1‑150 (MQ=60)
           TTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACG                                                                                                                                            >  SRR19973073.2493787/1‑150 (MQ=60)
            TCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGG                                                                                                                                           >  SRR19973073.1093684/1‑150 (MQ=60)
                   ATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCAC                                                                                                                                    >  SRR19973073.867533/1‑150 (MQ=60)
                   ATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCAC                                                                                                                                    >  SRR19973073.3189793/1‑150 (MQ=60)
                   ATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCAC                                                                                                                                    >  SRR19973073.4317954/1‑150 (MQ=60)
                     TTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGG                                                                                                                                  >  SRR19973073.3045900/1‑150 (MQ=60)
                     TTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGG                                                                                                                                  <  SRR19973073.1921995/150‑1 (MQ=60)
                     TTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGG                                                                                                                                  >  SRR19973073.771524/1‑150 (MQ=60)
                     TTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGG                                                                                                                                  <  SRR19973073.1922219/150‑1 (MQ=60)
                        GCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATA                                                                                                                               >  SRR19973073.543456/1‑150 (MQ=60)
                         CAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATAC                                                                                                                              <  SRR19973073.689011/150‑1 (MQ=60)
                         CAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATAC                                                                                                                              <  SRR19973073.688984/150‑1 (MQ=60)
                          AGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACG                                                                                                                             >  SRR19973073.258563/1‑150 (MQ=60)
                          AGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACG                                                                                                                             >  SRR19973073.258577/1‑150 (MQ=60)
                          AGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACG                                                                                                                             >  SRR19973073.1416310/1‑150 (MQ=60)
                          AGTAGTGGTAATAGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACACATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATAAG                                                                                                                             >  SRR19973073.258446/1‑150 (MQ=60)
                           GTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGA                                                                                                                            >  SRR19973073.2246565/1‑150 (MQ=60)
                           GTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGA                                                                                                                            >  SRR19973073.3607498/1‑150 (MQ=60)
                           GTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGACGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGA                                                                                                                            >  SRR19973073.1000738/1‑150 (MQ=60)
                               TGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTA                                                                                                                        >  SRR19973073.1499452/1‑150 (MQ=60)
                               TGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTA                                                                                                                        >  SRR19973073.2472723/1‑150 (MQ=60)
                                 GTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACA                                                                                                                      >  SRR19973073.1189601/1‑150 (MQ=60)
                                 GTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACA                                                                                                                      <  SRR19973073.2275540/150‑1 (MQ=60)
                                    ATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCA                                                                                                                   >  SRR19973073.3018065/1‑150 (MQ=60)
                                      GGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAAT                                                                                                                 <  SRR19973073.3075114/150‑1 (MQ=60)
                                      GGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAAT                                                                                                                 <  SRR19973073.1484636/150‑1 (MQ=60)
                                       GTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATA                                                                                                                >  SRR19973073.1355413/1‑150 (MQ=60)
                                       GTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATA                                                                                                                >  SRR19973073.1355394/1‑150 (MQ=60)
                                           TATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGA                                                                                                            >  SRR19973073.4238221/1‑150 (MQ=60)
                                            ATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGAT                                                                                                           >  SRR19973073.4103684/1‑150 (MQ=60)
                                              CCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCA                                                                                                         >  SRR19973073.327089/1‑150 (MQ=60)
                                              CCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCA                                                                                                         >  SRR19973073.327007/1‑150 (MQ=60)
                                               CAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAG                                                                                                        <  SRR19973073.2583792/150‑1 (MQ=60)
                                                 AACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGC                                                                                                      >  SRR19973073.1745578/1‑150 (MQ=60)
                                                 AACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGC                                                                                                      >  SRR19973073.4076121/1‑150 (MQ=60)
                                                 AACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGC                                                                                                      >  SRR19973073.2296103/1‑150 (MQ=60)
                                                  ACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCG                                                                                                     >  SRR19973073.3756876/1‑150 (MQ=60)
                                                    CACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCT                                                                                                   >  SRR19973073.849549/1‑150 (MQ=60)
                                                    CACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCT                                                                                                   >  SRR19973073.573043/1‑150 (MQ=60)
                                                    CACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCT                                                                                                   <  SRR19973073.1330216/150‑1 (MQ=60)
                                                     ACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTC                                                                                                  >  SRR19973073.2820571/1‑150 (MQ=60)
                                                      CGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCA                                                                                                 <  SRR19973073.4511401/150‑1 (MQ=60)
                                                        AACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAA                                                                                               >  SRR19973073.3451099/1‑150 (MQ=60)
                                                        AACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAA                                                                                               >  SRR19973073.2936781/1‑150 (MQ=60)
                                                          CGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGA                                                                                             <  SRR19973073.848765/150‑1 (MQ=60)
                                                          CGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGA                                                                                             <  SRR19973073.4005644/150‑1 (MQ=60)
                                                          CGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGA                                                                                             <  SRR19973073.1648411/150‑1 (MQ=60)
                                                            CGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAAC                                                                                           <  SRR19973073.457656/150‑1 (MQ=60)
                                                               TCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCC                                                                                        >  SRR19973073.4493111/1‑150 (MQ=60)
                                                                CGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCC                                                                                       <  SRR19973073.4179851/150‑1 (MQ=60)
                                                                CGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCC                                                                                       <  SRR19973073.1308664/150‑1 (MQ=60)
                                                                CGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACAGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCC                                                                                       >  SRR19973073.2473370/1‑150 (MQ=60)
                                                                      TATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGC                                                                                 <  SRR19973073.2881166/150‑1 (MQ=60)
                                                                       ATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCG                                                                                >  SRR19973073.1794288/1‑150 (MQ=60)
                                                                         CGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTT                                                                              >  SRR19973073.4373492/1‑150 (MQ=60)
                                                                         CGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTT                                                                              <  SRR19973073.3698429/150‑1 (MQ=60)
                                                                            AGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGC                                                                           >  SRR19973073.3420482/1‑150 (MQ=60)
                                                                            AGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGC                                                                           >  SRR19973073.3434403/1‑150 (MQ=60)
                                                                                GATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACG                                                                       >  SRR19973073.4351852/1‑150 (MQ=60)
                                                                                   TTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAG                                                                    >  SRR19973073.1667772/1‑150 (MQ=60)
                                                                                   TTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAG                                                                    >  SRR19973073.1667898/1‑150 (MQ=60)
                                                                                     CTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAG                                                                  <  SRR19973073.2799360/150‑1 (MQ=60)
                                                                                     CTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAG                                                                  <  SRR19973073.3409155/150‑1 (MQ=60)
                                                                                     CTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAG                                                                  <  SRR19973073.2798495/150‑1 (MQ=60)
                                                                                          AAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACT                                                             >  SRR19973073.2164999/1‑150 (MQ=60)
                                                                                          AAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCAACATGCGTTCGCCACGCAGAGTTACT                                                             <  SRR19973073.3258600/150‑1 (MQ=60)
                                                                                           AGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTT                                                            <  SRR19973073.3594249/150‑1 (MQ=60)
                                                                                           AGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTT                                                            >  SRR19973073.3658852/1‑150 (MQ=60)
                                                                                           AGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTT                                                            <  SRR19973073.2651087/150‑1 (MQ=60)
                                                                                           AGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTT                                                            >  SRR19973073.3658881/1‑150 (MQ=60)
                                                                                            GATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTT                                                           >  SRR19973073.1537369/1‑150 (MQ=60)
                                                                                            GATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTT                                                           >  SRR19973073.32715/1‑150 (MQ=60)
                                                                                            GATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTT                                                           >  SRR19973073.3209045/1‑150 (MQ=60)
                                                                                             ATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTGTA                                                          >  SRR19973073.649973/1‑150 (MQ=60)
                                                                                             ATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTA                                                          >  SRR19973073.2326639/1‑150 (MQ=60)
                                                                                                   TGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCG                                                    >  SRR19973073.1312780/1‑150 (MQ=60)
                                                                                                    GCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGA                                                   >  SRR19973073.3545442/1‑150 (MQ=60)
                                                                                                         CGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGA                                              >  SRR19973073.3245725/1‑150 (MQ=60)
                                                                                                         CGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGA                                              <  SRR19973073.1398337/150‑1 (MQ=60)
                                                                                                         CGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGA                                              >  SRR19973073.3697305/1‑150 (MQ=60)
                                                                                                          GGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGAT                                             >  SRR19973073.3222146/1‑150 (MQ=60)
                                                                                                          GGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGAT                                             >  SRR19973073.2686292/1‑150 (MQ=60)
                                                                                                             CACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGC                                          <  SRR19973073.4487992/150‑1 (MQ=60)
                                                                                                              ACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGATAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCC                                         >  SRR19973073.3123376/1‑150 (MQ=60)
                                                                                                              ACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGATAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCC                                         >  SRR19973073.3123379/1‑150 (MQ=60)
                                                                                                                CCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCT                                       <  SRR19973073.623127/150‑1 (MQ=60)
                                                                                                                CCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCT                                       <  SRR19973073.623124/150‑1 (MQ=60)
                                                                                                                     CTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGG                                  >  SRR19973073.1146624/1‑150 (MQ=60)
                                                                                                                      TGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGA                                 <  SRR19973073.1222452/150‑1 (MQ=60)
                                                                                                                      TGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGA                                 <  SRR19973073.1222466/150‑1 (MQ=60)
                                                                                                                      TGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGA                                 <  SRR19973073.1658255/150‑1 (MQ=60)
                                                                                                                       GTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGAT                                <  SRR19973073.304288/150‑1 (MQ=60)
                                                                                                                        TAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATT                               <  SRR19973073.4074854/150‑1 (MQ=60)
                                                                                                                        TAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATT                               <  SRR19973073.4074833/150‑1 (MQ=60)
                                                                                                                        TAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATT                               <  SRR19973073.2018009/150‑1 (MQ=60)
                                                                                                                        TAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATT                               >  SRR19973073.4436568/1‑150 (MQ=60)
                                                                                                                         AGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTT                              >  SRR19973073.3656642/1‑150 (MQ=60)
                                                                                                                         AGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTT                              <  SRR19973073.289526/150‑1 (MQ=60)
                                                                                                                            TACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGA                           <  SRR19973073.692154/150‑1 (MQ=60)
                                                                                                                              CCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAG                         >  SRR19973073.804735/1‑150 (MQ=60)
                                                                                                                               CACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGC                        >  SRR19973073.4487680/1‑150 (MQ=60)
                                                                                                                               CACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGC                        <  SRR19973073.3856628/150‑1 (MQ=60)
                                                                                                                                 CGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCT                      >  SRR19973073.2457972/1‑150 (MQ=60)
                                                                                                                                    CCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCA                   >  SRR19973073.4243216/1‑150 (MQ=60)
                                                                                                                                    CCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCA                   <  SRR19973073.1499514/150‑1 (MQ=60)
                                                                                                                                    CCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCA                   <  SRR19973073.1499521/150‑1 (MQ=60)
                                                                                                                                     CGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAA                  >  SRR19973073.3811771/1‑150 (MQ=60)
                                                                                                                                      GTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAAC                 >  SRR19973073.2905947/1‑150 (MQ=60)
                                                                                                                                      GTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAAC                 >  SRR19973073.2431393/1‑150 (MQ=60)
                                                                                                                                        CGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAG               <  SRR19973073.3758977/150‑1 (MQ=60)
                                                                                                                                        CGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAG               <  SRR19973073.3359602/150‑1 (MQ=60)
                                                                                                                                          AAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGAT             >  SRR19973073.884549/1‑150 (MQ=60)
                                                                                                                                          AAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGAT             >  SRR19973073.92098/1‑150 (MQ=60)
                                                                                                                                          AAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGAT             >  SRR19973073.1732359/1‑150 (MQ=60)
                                                                                                                                           AAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATT            <  SRR19973073.2593912/150‑1 (MQ=60)
                                                                                                                                            AGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTT           <  SRR19973073.2526760/150‑1 (MQ=60)
                                                                                                                                            AGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTT           <  SRR19973073.1347901/150‑1 (MQ=60)
                                                                                                                                            AGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTT           <  SRR19973073.1347885/150‑1 (MQ=60)
                                                                                                                                              ACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTTGC         >  SRR19973073.2456021/1‑150 (MQ=60)
                                                                                                                                              ACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTTGC         <  SRR19973073.2624561/150‑1 (MQ=60)
                                                                                                                                                 TAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTTGCGTG      <  SRR19973073.2733795/150‑1 (MQ=60)
                                                                                                                                                     TGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTTGCGTGCTTT  <  SRR19973073.875472/150‑1 (MQ=60)
                                                                                                                                                     TGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTTGCGTGCTTT  <  SRR19973073.3232213/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
GCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTTGCGTGCTTT  >  CP009273/3440295‑3440593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑