Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,440,444 G→T 100% S118S (TCC→TCA)  rplE ← 50S ribosomal subunit protein L5

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,440,4440GT100.0% 709.9 / NA 213S118S (TCC→TCA) rplE50S ribosomal subunit protein L5
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (115/98);  total (115/98)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 213 total aligned reads displayed.

GCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTTGCG  >  CP009273/3440300‑3440587
                                                                                                                                                |                                                                                                                                               
 ccTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACa                                                                                                                                             <  1:409705/148‑1 (MQ=255)
 ccTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACa                                                                                                                                             >  1:887102/1‑148 (MQ=255)
  cttcttCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAg                                                                                                                                            <  2:1436704/148‑1 (MQ=255)
   ttcttcGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAgg                                                                                                                                           >  1:879293/1‑148 (MQ=255)
   ttcttcGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAgg                                                                                                                                           >  2:3245436/1‑148 (MQ=255)
      ttcGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCa                                                                                                                                        <  2:787378/148‑1 (MQ=255)
       tcGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCAc                                                                                                                                       >  2:1902278/1‑148 (MQ=255)
          tCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGa                                                                                                                                    >  2:2617250/1‑148 (MQ=255)
             gATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGt                                                                                                                                 <  1:2138321/148‑1 (MQ=255)
              aTTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGACGTc                                                                                                                                <  1:2120551/148‑1 (MQ=255)
              aTTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTc                                                                                                                                >  1:831632/1‑148 (MQ=255)
              aTTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTc                                                                                                                                <  1:2770785/148‑1 (MQ=255)
               tttCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCa                                                                                                                               <  2:2896907/148‑1 (MQ=255)
               tttCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCa                                                                                                                               <  2:2945262/148‑1 (MQ=255)
                ttCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTAACACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCAc                                                                                                                              >  1:3005702/1‑148 (MQ=255)
                   gCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGa                                                                                                                           <  2:780933/148‑1 (MQ=255)
                       tagtGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGACGTCACGGATACg                                                                                                                       >  1:3022929/1‑148 (MQ=255)
                       tagtGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACg                                                                                                                       >  2:2811041/1‑148 (MQ=255)
                       tagtGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACg                                                                                                                       >  1:1644373/1‑148 (MQ=255)
                       tagtGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACg                                                                                                                       <  2:1344189/148‑1 (MQ=255)
                             taatggtaatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTAc                                                                                                                 <  1:142230/148‑1 (MQ=255)
                                 ggtaatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCa                                                                                                             <  2:2645570/148‑1 (MQ=255)
                                  gtaatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCaa                                                                                                            >  1:2975790/1‑148 (MQ=255)
                                   taatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAAt                                                                                                           <  2:124326/148‑1 (MQ=255)
                                     atatCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAg                                                                                                         <  1:2030757/148‑1 (MQ=255)
                                        tCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGa                                                                                                      <  1:291087/148‑1 (MQ=255)
                                        tCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAAGAGTGa                                                                                                      >  2:666030/1‑148 (MQ=255)
                                         ccAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGAt                                                                                                     >  1:639429/1‑148 (MQ=255)
                                           aaaCCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCa                                                                                                   <  2:1619737/148‑1 (MQ=255)
                                            aaCCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAg                                                                                                  <  2:1566555/148‑1 (MQ=255)
                                            aaCCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAg                                                                                                  >  1:2071626/1‑148 (MQ=255)
                                            aaCCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAg                                                                                                  >  1:3184733/1‑148 (MQ=255)
                                              ccACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGgc                                                                                                >  1:2371757/1‑148 (MQ=255)
                                               cACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGgcg                                                                                               <  2:120949/148‑1 (MQ=255)
                                                 cGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCt                                                                                             >  1:3352410/1‑148 (MQ=255)
                                                    aCGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCaa                                                                                          <  1:262271/148‑1 (MQ=255)
                                                     cgcgGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCaaa                                                                                         >  1:3395470/1‑148 (MQ=255)
                                                      gcgGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAg                                                                                        <  1:2599828/148‑1 (MQ=255)
                                                       cgGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGa                                                                                       <  1:2049055/148‑1 (MQ=255)
                                                         gTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAAc                                                                                     >  2:1997672/1‑148 (MQ=255)
                                                          tCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACt                                                                                    <  1:2554198/148‑1 (MQ=255)
                                                          tCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACt                                                                                    >  1:573853/1‑148 (MQ=255)
                                                               tttATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCcac                                                                               <  1:1042582/148‑1 (MQ=255)
                                                                ttATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCcaca                                                                              <  1:2449079/148‑1 (MQ=255)
                                                                ttATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCcaca                                                                              >  2:2486106/1‑148 (MQ=255)
                                                                    cGTAGTCGATTTCTGGGAAGATGATCTTCTCACGGAAAACCAAGCTGTAGTTACCACGACCCACGAAAGACTTAGCTGACAGGCCACCGAAGTCACGGATACGATGTACAGCAATAGTGATCAGGCGCTCAAAGAACTACcacaatcg                                                                          >  1:3296662/1‑144 (MQ=255)
                                                                    cGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCg                                                                          >  2:2337970/1‑148 (MQ=255)
                                                                     gTAGTCGATTTCTGGGAAGATGATCTGCTCAAGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCAAGGATACGAGGTACATCAATAGTGATCAGGCGCTCAAAGAACTCCCACAttagt                                                                         >  1:2941550/1‑144 (MQ=255)
                                                                      tAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGtt                                                                        <  2:879293/148‑1 (MQ=255)
                                                                         tCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGc                                                                     >  1:1046715/1‑148 (MQ=255)
                                                                           gATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCa                                                                   >  1:1700243/1‑148 (MQ=255)
                                                                            aTTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCAc                                                                  >  1:672232/1‑148 (MQ=255)
                                                                            aTTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCAc                                                                  >  1:2662653/1‑148 (MQ=255)
                                                                             tttCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACg                                                                 >  1:2177513/1‑148 (MQ=255)
                                                                              ttCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGc                                                                >  2:2531701/1‑148 (MQ=255)
                                                                                  gggAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCagag                                                            <  1:2468994/148‑1 (MQ=255)
                                                                                   ggAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGt                                                           <  1:3840/148‑1 (MQ=255)
                                                                                    gAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGtt                                                          >  2:2003661/1‑148 (MQ=255)
                                                                                    gAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGtt                                                          <  2:3473824/148‑1 (MQ=255)
                                                                                     aaGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTa                                                         <  1:2679017/148‑1 (MQ=255)
                                                                                     aaGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTa                                                         <  2:2247589/148‑1 (MQ=255)
                                                                                     aaGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTa                                                         <  2:482982/148‑1 (MQ=255)
                                                                                      aGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTAc                                                        >  2:1488120/1‑148 (MQ=255)
                                                                                       gatgatCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACt                                                       <  1:1512103/148‑1 (MQ=255)
                                                                                        atgatCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCACTAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACtt                                                      >  2:1982718/1‑148 (MQ=255)
                                                                                         tgatCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACttt                                                     <  1:1865514/148‑1 (MQ=255)
                                                                                          gatCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTa                                                    >  1:181028/1‑148 (MQ=255)
                                                                                            tCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACa                                                  >  1:2801443/1‑148 (MQ=255)
                                                                                                cTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCg                                              <  2:1803684/148‑1 (MQ=255)
                                                                                                  cACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGAt                                            >  1:590052/1‑148 (MQ=255)
                                                                                                     ggACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCgg                                         <  2:3184733/148‑1 (MQ=255)
                                                                                                     ggACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCgg                                         <  2:2071626/148‑1 (MQ=255)
                                                                                                       acacCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGAt                                       <  2:416690/148‑1 (MQ=255)
                                                                                                       acacCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGAt                                       >  1:108586/1‑148 (MQ=255)
                                                                                                         acCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAg                                     >  1:75850/1‑148 (MQ=255)
                                                                                                          cccATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGc                                    >  1:515115/1‑148 (MQ=255)
                                                                                                          cccATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGc                                    >  1:346092/1‑148 (MQ=255)
                                                                                                          cccATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGc                                    >  1:1320032/1‑148 (MQ=255)
                                                                                                           ccATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGcc                                   >  2:237533/1‑148 (MQ=255)
                                                                                                             aTGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCt                                 >  2:209406/1‑148 (MQ=255)
                                                                                                              tGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTg                                >  1:347215/1‑148 (MQ=255)
                                                                                                              tGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTg                                >  2:3359855/1‑148 (MQ=255)
                                                                                                              tGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTg                                <  2:1777584/148‑1 (MQ=255)
                                                                                                                cTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGAc                              >  2:2135733/1‑148 (MQ=255)
                                                                                                                   tAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGa                           >  2:1328256/1‑148 (MQ=255)
                                                                                                                   tAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGa                           <  2:1497457/148‑1 (MQ=255)
                                                                                                                     gTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGAtt                         <  2:863390/148‑1 (MQ=255)
                                                                                                                      ttACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGAttt                        <  1:1738420/148‑1 (MQ=255)
                                                                                                                            cGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGc                  >  1:1251254/1‑148 (MQ=255)
                                                                                                                              aCCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCt                <  2:2886504/148‑1 (MQ=255)
                                                                                                                                  tCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATTCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCaa            >  2:1847657/1‑148 (MQ=255)
                                                                                                                                     aaaGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAg         >  1:2238048/1‑148 (MQ=255)
                                                                                                                                      aaGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAAgaga        <  2:3352410/148‑1 (MQ=255)
                                                                                                                                      aaGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGa        <  1:196339/148‑1 (MQ=255)
                                                                                                                                      aaGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATAAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGa        >  2:3175938/1‑148 (MQ=255)
                                                                                                                                       aGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGAt       <  1:751633/148‑1 (MQ=255)
                                                                                                                                        gACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGAtt      >  1:2314217/1‑148 (MQ=255)
                                                                                                                                           ttAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTTGc   >  2:3060264/1‑148 (MQ=255)
                                                                                                                                           ttAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTTGc   >  2:2082661/1‑148 (MQ=255)
                                                                                                                                            tAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTTGCg  <  2:2552228/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
GCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTTGCG  >  CP009273/3440300‑3440587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTTGCGTGCTTT  >  CP009273/3440295‑3440593
                                                                                                                                                     |                                                                                                                                                     
GCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCG                                                                                                                                                       <  SRR19973065.2974613/150‑1 (MQ=60)
GCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCG                                                                                                                                                       <  SRR19973065.1214860/150‑1 (MQ=60)
 CGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTG                                                                                                                                                      <  SRR19973065.687113/150‑1 (MQ=60)
 CGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTG                                                                                                                                                      >  SRR19973065.3141377/1‑150 (MQ=60)
    GGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACA                                                                                                                                                   >  SRR19973065.892736/1‑150 (MQ=60)
     GCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAG                                                                                                                                                  <  SRR19973065.1903436/150‑1 (MQ=60)
      CCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGG                                                                                                                                                 <  SRR19973065.412576/150‑1 (MQ=60)
      CCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGG                                                                                                                                                 <  SRR19973065.412497/150‑1 (MQ=60)
      CCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGG                                                                                                                                                 >  SRR19973065.884896/1‑150 (MQ=60)
                 AGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTC                                                                                                                                      >  SRR19973065.837063/1‑150 (MQ=60)
                 AGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTC                                                                                                                                      >  SRR19973065.1995009/1‑150 (MQ=60)
                  GATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCA                                                                                                                                     <  SRR19973065.2152078/150‑1 (MQ=60)
                  GATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCA                                                                                                                                     <  SRR19973065.1854905/150‑1 (MQ=60)
                   ATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGACGTCAC                                                                                                                                    <  SRR19973065.2134201/150‑1 (MQ=60)
                   ATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCAC                                                                                                                                    <  SRR19973065.2134340/150‑1 (MQ=60)
                   ATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCAC                                                                                                                                    <  SRR19973065.2134200/150‑1 (MQ=60)
                   ATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCAC                                                                                                                                    <  SRR19973065.2787994/150‑1 (MQ=60)
                   ATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTAACACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCAC                                                                                                                                    >  SRR19973065.3024114/1‑150 (MQ=60)
                     TTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGG                                                                                                                                  <  SRR19973065.3526761/150‑1 (MQ=60)
                         CAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATAC                                                                                                                              <  SRR19973065.967104/150‑1 (MQ=60)
                          AGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGACGTCACGGATACG                                                                                                                             >  SRR19973065.3041455/1‑150 (MQ=60)
                          AGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACG                                                                                                                             >  SRR19973065.2952431/1‑150 (MQ=60)
                          AGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACG                                                                                                                             >  SRR19973065.1655411/1‑150 (MQ=60)
                          AGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACG                                                                                                                             >  SRR19973065.2245940/1‑150 (MQ=60)
                            TAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAG                                                                                                                           <  SRR19973065.301181/150‑1 (MQ=60)
                                 GTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACA                                                                                                                      >  SRR19973065.1451347/1‑150 (MQ=60)
                                  TAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAG                                                                                                                     <  SRR19973065.143440/150‑1 (MQ=60)
                                     TGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAA                                                                                                                  >  SRR19973065.2993975/1‑150 (MQ=60)
                                     TGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAA                                                                                                                  >  SRR19973065.2993998/1‑150 (MQ=60)
                                      GGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAAT                                                                                                                 <  SRR19973065.799786/150‑1 (MQ=60)
                                          ATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTG                                                                                                             >  SRR19973065.2620466/1‑150 (MQ=60)
                                          ATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTG                                                                                                             <  SRR19973065.260751/150‑1 (MQ=60)
                                          ATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTG                                                                                                             <  SRR19973065.2043790/150‑1 (MQ=60)
                                            ATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGAT                                                                                                           >  SRR19973065.643618/1‑150 (MQ=60)
                                            ATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGAT                                                                                                           <  SRR19973065.107757/150‑1 (MQ=60)
                                             TCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATC                                                                                                          <  SRR19973065.292979/150‑1 (MQ=60)
                                               CAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAG                                                                                                        >  SRR19973065.3203964/1‑150 (MQ=60)
                                               CAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATAAG                                                                                                        >  SRR19973065.2084842/1‑150 (MQ=60)
                                                AAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGG                                                                                                       <  SRR19973065.3409116/150‑1 (MQ=60)
                                                 AACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGC                                                                                                      >  SRR19973065.2384285/1‑150 (MQ=60)
                                                 AACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGC                                                                                                      >  SRR19973065.2386553/1‑150 (MQ=60)
                                                    CACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCT                                                                                                   >  SRR19973065.3372896/1‑150 (MQ=60)
                                                     ACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTC                                                                                                  <  SRR19973065.2553613/150‑1 (MQ=60)
                                                      CGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCA                                                                                                 <  SRR19973065.2500817/150‑1 (MQ=60)
                                                        AACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAA                                                                                               >  SRR19973065.3416232/1‑150 (MQ=60)
                                                         ACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAG                                                                                              <  SRR19973065.264072/150‑1 (MQ=60)
                                                           GCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAA                                                                                            <  SRR19973065.2615975/150‑1 (MQ=60)
                                                            CGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAAC                                                                                           <  SRR19973065.2062134/150‑1 (MQ=60)
                                                            CGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAAC                                                                                           <  SRR19973065.2062149/150‑1 (MQ=60)
                                                             GGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACT                                                                                          >  SRR19973065.577466/1‑150 (MQ=60)
                                                              GTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTC                                                                                         <  SRR19973065.3249887/150‑1 (MQ=60)
                                                               TCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCC                                                                                        <  SRR19973065.2570177/150‑1 (MQ=60)
                                                                CGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCC                                                                                       <  SRR19973065.266546/150‑1 (MQ=60)
                                                                 GACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCA                                                                                      <  SRR19973065.1706275/150‑1 (MQ=60)
                                                                    TTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACAT                                                                                   <  SRR19973065.2127419/150‑1 (MQ=60)
                                                                    TTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACAT                                                                                   <  SRR19973065.1049474/150‑1 (MQ=60)
                                                                     TTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATG                                                                                  <  SRR19973065.2464510/150‑1 (MQ=60)
                                                                     TTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATG                                                                                  <  SRR19973065.2463066/150‑1 (MQ=60)
                                                                       ATCGTAGTCGATTTCTGGGAAGATGATCTTCTCACGGAAAACCAAGCTGTAGTTACCACGACCCACGAAAGACTTAGCTGACAGGCCACCGAAGTCACGGATACGATGTACAGCAATAGTGATCAGGCGCTCAAAGAACTACCACAatcg                                                                                >  SRR19973065.3316740/1‑146 (MQ=60)
                                                                       ATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCG                                                                                >  SRR19973065.2847554/1‑150 (MQ=60)
                                                                        TCGTAGTCGATTTCTGGGAAGATGATCTGCTCAAGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCAAGGATACGAGGTACATCAATAGTGATCAGGCGCTCAAAGAACTCCCACATtagt                                                                               >  SRR19973065.2959408/1‑146 (MQ=60)
                                                                         CGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTT                                                                              >  SRR19973065.1316651/1‑150 (MQ=60)
                                                                         CGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTT                                                                              <  SRR19973065.3331453/150‑1 (MQ=60)
                                                                          GTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTC                                                                             <  SRR19973065.2767862/150‑1 (MQ=60)
                                                                          GTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTC                                                                             >  SRR19973065.2834521/1‑150 (MQ=60)
                                                                            AGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGC                                                                           >  SRR19973065.1053637/1‑150 (MQ=60)
                                                                              TCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCA                                                                         >  SRR19973065.1711577/1‑150 (MQ=60)
                                                                              TCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCA                                                                         <  SRR19973065.1526555/150‑1 (MQ=60)
                                                                               CGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCAC                                                                        >  SRR19973065.2679131/1‑150 (MQ=60)
                                                                               CGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCAC                                                                        >  SRR19973065.676691/1‑150 (MQ=60)
                                                                               CGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCAC                                                                        >  SRR19973065.676712/1‑150 (MQ=60)
                                                                                GATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACG                                                                       <  SRR19973065.1672493/150‑1 (MQ=60)
                                                                                GATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACG                                                                       <  SRR19973065.3423146/150‑1 (MQ=60)
                                                                                GATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACG                                                                       >  SRR19973065.2191476/1‑150 (MQ=60)
                                                                                   TTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAG                                                                    <  SRR19973065.241862/150‑1 (MQ=60)
                                                                                   TTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAG                                                                    <  SRR19973065.241848/150‑1 (MQ=60)
                                                                                   TTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAG                                                                    <  SRR19973065.240231/150‑1 (MQ=60)
                                                                                     CTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAG                                                                  >  SRR19973065.3402498/1‑150 (MQ=60)
                                                                                      TGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGT                                                                 <  SRR19973065.854663/150‑1 (MQ=60)
                                                                                       GGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTT                                                                <  SRR19973065.2484544/150‑1 (MQ=60)
                                                                                        GGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTA                                                               >  SRR19973065.2813186/1‑150 (MQ=60)
                                                                                        GGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTA                                                               >  SRR19973065.1051713/1‑150 (MQ=60)
                                                                                        GGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTA                                                               >  SRR19973065.1404126/1‑150 (MQ=60)
                                                                                        GGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTA                                                               >  SRR19973065.41813/1‑150 (MQ=60)
                                                                                        GGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTA                                                               <  SRR19973065.3891/150‑1 (MQ=60)
                                                                                         GAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTAC                                                              <  SRR19973065.2475812/150‑1 (MQ=60)
                                                                                          AAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACT                                                             <  SRR19973065.2695628/150‑1 (MQ=60)
                                                                                          AAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACT                                                             >  SRR19973065.3060021/1‑150 (MQ=60)
                                                                                          AAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACT                                                             <  SRR19973065.930879/150‑1 (MQ=60)
                                                                                            GATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTT                                                           <  SRR19973065.1521960/150‑1 (MQ=60)
                                                                                             ATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTA                                                          >  SRR19973065.182460/1‑150 (MQ=60)
                                                                                              TGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTAC                                                         <  SRR19973065.1877792/150‑1 (MQ=60)
                                                                                               GATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACA                                                        >  SRR19973065.2818794/1‑150 (MQ=60)
                                                                                                     CTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGAT                                                  >  SRR19973065.594832/1‑150 (MQ=60)
                                                                                                     CTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGAT                                                  >  SRR19973065.593710/1‑150 (MQ=60)
                                                                                                     CTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGAT                                                  >  SRR19973065.593673/1‑150 (MQ=60)
                                                                                                       CACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCG                                                <  SRR19973065.627280/150‑1 (MQ=60)
                                                                                                          GGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGAT                                             >  SRR19973065.109572/1‑150 (MQ=60)
                                                                                                            ACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAG                                           >  SRR19973065.75998/1‑150 (MQ=60)
                                                                                                            ACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAG                                           >  SRR19973065.76619/1‑150 (MQ=60)
                                                                                                             CACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGC                                          >  SRR19973065.1328762/1‑150 (MQ=60)
                                                                                                             CACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGC                                          >  SRR19973065.518524/1‑150 (MQ=60)
                                                                                                             CACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGC                                          >  SRR19973065.2164755/1‑150 (MQ=60)
                                                                                                             CACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGC                                          >  SRR19973065.348517/1‑150 (MQ=60)
                                                                                                              ACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCC                                         <  SRR19973065.885789/150‑1 (MQ=60)
                                                                                                                CCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCT                                       <  SRR19973065.1114782/150‑1 (MQ=60)
                                                                                                                 CATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTG                                      >  SRR19973065.349645/1‑150 (MQ=60)
                                                                                                                 CATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTG                                      >  SRR19973065.349639/1‑150 (MQ=60)
                                                                                                                   TGCTGTAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGAC                                    <  SRR19973065.2063960/150‑1 (MQ=60)
                                                                                                                        TAGTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATT                               >  SRR19973065.565748/1‑150 (MQ=60)
                                                                                                                          GTTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTT                             >  SRR19973065.302746/1‑150 (MQ=60)
                                                                                                                           TTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTG                            <  SRR19973065.1749907/150‑1 (MQ=60)
                                                                                                                           TTACCACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTG                            <  SRR19973065.1749945/150‑1 (MQ=60)
                                                                                                                               CACGACCGTCGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGC                        >  SRR19973065.1259492/1‑150 (MQ=60)
                                                                                                                                        CGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAG               >  SRR19973065.2252342/1‑150 (MQ=60)
                                                                                                                                        CGAAAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAG               >  SRR19973065.2252277/1‑150 (MQ=60)
                                                                                                                                           AAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATT            <  SRR19973065.197870/150‑1 (MQ=60)
                                                                                                                                           AAGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATT            >  SRR19973065.2328730/1‑150 (MQ=60)
                                                                                                                                            AGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTT           <  SRR19973065.1747714/150‑1 (MQ=60)
                                                                                                                                            AGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTT           <  SRR19973065.2685238/150‑1 (MQ=60)
                                                                                                                                            AGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTT           <  SRR19973065.756600/150‑1 (MQ=60)
                                                                                                                                            AGACTTAGCTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTT           <  SRR19973065.756646/150‑1 (MQ=60)
                                                                                                                                                    CTGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTTGCGTGCTT   <  SRR19973065.2352568/150‑1 (MQ=60)
                                                                                                                                                     TGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTTGCGTGCTTT  >  SRR19973065.563097/1‑150 (MQ=60)
                                                                                                                                                     TGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTTGCGTGCTTT  <  SRR19973065.455275/150‑1 (MQ=60)
                                                                                                                                                     TGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTTGCGTGCTTT  <  SRR19973065.1914781/150‑1 (MQ=60)
                                                                                                                                                     TGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTTGCGTGCTTT  <  SRR19973065.31401/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
GCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCACATGCGTTCGCCACGCAGAGTTACTTTACAGCCGATCGGATAGCCCTGACGGATTTTGAAGCCTGCAACAGATTTGCGTGCTTT  >  CP009273/3440295‑3440593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑