Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,835,154 G→A 9.4% intergenic (‑55/+156) nepI ← / ← yicN putative transporter/DUF1198 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,835,1540GA 9.4% 829.9 / 54.7 256intergenic (‑55/+156)nepI/yicNputative transporter/DUF1198 family protein
Reads supporting (aligned to +/- strand):  ref base G (93/139);  new base A (9/15);  total (102/154)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 270 total aligned reads displayed.

AGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGGCGCGGTCTGTAGGCTGATAAGACG  >  CP009273/3835005‑3835301
                                                                                                                                                     |                                                                                                                                                   
   cAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAAtgtgt                                                                                                                                                  <  2:960305/150‑1 (MQ=255)
   cAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAAtgtgt                                                                                                                                                  <  1:1319685/150‑1 (MQ=255)
   cAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAAtgtgt                                                                                                                                                  <  1:3732116/150‑1 (MQ=255)
   cAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAAtgtgt                                                                                                                                                  <  1:1695814/150‑1 (MQ=255)
    aGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAAtgtgtg                                                                                                                                                 <  1:2266735/150‑1 (MQ=255)
    aGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAAtgtgtg                                                                                                                                                 <  1:2163274/150‑1 (MQ=255)
    aGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAAAAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAAtgtgtg                                                                                                                                                 >  2:2357829/1‑150 (MQ=255)
      gCGACACAAACCGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGAc                                                                                                                                               <  1:710524/150‑1 (MQ=255)
       cGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACg                                                                                                                                              <  1:2035559/150‑1 (MQ=255)
          cacaAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCAt                                                                                                                                           >  2:4457611/1‑150 (MQ=255)
           acacaccGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCAtt                                                                                                                                          <  2:2445430/144‑1 (MQ=255)
             aaaaCGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTa                                                                                                                                        >  1:2880654/1‑150 (MQ=255)
             aaaaCGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTa                                                                                                                                        >  2:1794089/1‑150 (MQ=255)
               caCGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATATGTGACGCATTTAAc                                                                                                                                      <  2:2811453/149‑1 (MQ=255)
                ccGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACg                                                                                                                                     <  2:4522555/149‑1 (MQ=255)
                   ccACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGttt                                                                                                                                  <  2:3680994/150‑1 (MQ=255)
                   ccACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGttt                                                                                                                                  <  2:430867/150‑1 (MQ=255)
                    cACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGtttt                                                                                                                                 >  2:3042086/1‑150 (MQ=255)
                    cACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGtttt                                                                                                                                 <  1:3332089/150‑1 (MQ=255)
                    cACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGtttt                                                                                                                                 <  1:1386215/150‑1 (MQ=255)
                         agaAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCaa                                                                                                                            <  1:2031381/150‑1 (MQ=255)
                          gaAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAAc                                                                                                                           <  2:417027/150‑1 (MQ=255)
                             aaCGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACttt                                                                                                                        <  1:2070627/150‑1 (MQ=255)
                                   tGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCTCTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGgt                                                                                                                  >  2:756685/1‑150 (MQ=255)
                                     aCCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGgtgt                                                                                                                <  2:2147802/150‑1 (MQ=255)
                                      ccAGTTCGGTCGGGTGGTGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGtt                                                                                                               <  2:4200198/150‑1 (MQ=255)
                                        aGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATATGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGc                                                                                                             <  1:3953678/150‑1 (MQ=255)
                                         gTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCt                                                                                                            <  1:4449931/150‑1 (MQ=255)
                                          ttCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCtt                                                                                                           <  1:3275925/150‑1 (MQ=255)
                                                cggGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTg                                                                                                     >  2:1775629/1‑150 (MQ=255)
                                                 gggTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTgg                                                                                                    >  1:3086003/1‑150 (MQ=255)
                                                  ggTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGa                                                                                                   >  2:253465/1‑150 (MQ=255)
                                                   gTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATATGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAg                                                                                                  <  1:370745/150‑1 (MQ=255)
                                                    tGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGt                                                                                                 >  2:144211/1‑150 (MQ=255)
                                                         gCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGa                                                                                            <  1:741023/150‑1 (MQ=255)
                                                         gCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGATTCATGGGATAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGa                                                                                            <  1:4436603/150‑1 (MQ=255)
                                                            tCCGCGCCGCGGTTTTCGGCACTAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGCGCTGGAGTTAAGATCg                                                                                         <  2:2541402/150‑1 (MQ=255)
                                                             ccgcgccgcgGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGa                                                                                        >  2:942448/1‑150 (MQ=255)
                                                             ccgcgccgcgGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGa                                                                                        >  2:3960699/1‑150 (MQ=255)
                                                             ccgcgccgcgGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGa                                                                                        <  2:3933460/150‑1 (MQ=255)
                                                                cgccgcgGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAAc                                                                                     <  1:2727297/150‑1 (MQ=255)
                                                                cgccgcgGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAAc                                                                                     >  2:3302414/1‑150 (MQ=255)
                                                                cgccgcgGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAAc                                                                                     <  1:237254/150‑1 (MQ=255)
                                                                 gccgcgGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACg                                                                                    <  1:4101841/150‑1 (MQ=255)
                                                                      ggTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACa                                                                               <  1:3009602/150‑1 (MQ=255)
                                                                      ggTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATATGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACa                                                                               >  1:1463422/1‑150 (MQ=255)
                                                                           tCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCa                                                                          >  2:1383491/1‑150 (MQ=255)
                                                                                 aTAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGAtt                                                                    <  1:43542/150‑1 (MQ=255)
                                                                                     aTTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCg                                                                <  1:784917/150‑1 (MQ=255)
                                                                                     aTTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCg                                                                <  2:3669984/150‑1 (MQ=255)
                                                                                      ttCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATATGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCgg                                                               >  1:2102798/1‑150 (MQ=255)
                                                                                        cACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGAt                                                             >  1:4236733/1‑150 (MQ=255)
                                                                                        cACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGAt                                                             >  1:1987754/1‑150 (MQ=255)
                                                                                          cTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGAt                                                             <  1:3219631/148‑1 (MQ=255)
                                                                                            cATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATgcgg                                                         <  2:453600/150‑1 (MQ=255)
                                                                                             aTGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACTGAACAGGGCAAAGATTACCGGATgcggc                                                        >  2:1610967/1‑150 (MQ=255)
                                                                                                gtgtTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTg                                                     >  2:1591681/1‑150 (MQ=255)
                                                                                                  gtTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGaa                                                   <  1:3354689/150‑1 (MQ=255)
                                                                                                   ttACCCGAGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAAc                                                  <  1:2151500/150‑1 (MQ=255)
                                                                                                   ttACCCGAGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAAc                                                  <  1:2151066/150‑1 (MQ=255)
                                                                                                      cccGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTc                                               <  2:125537/150‑1 (MQ=255)
                                                                                                       ccGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCt                                              >  1:948170/1‑150 (MQ=255)
                                                                                                       ccGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCt                                              <  2:579373/150‑1 (MQ=255)
                                                                                                       ccGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCt                                              <  2:579593/150‑1 (MQ=255)
                                                                                                             cATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCa                                        <  1:588739/150‑1 (MQ=255)
                                                                                                              aTGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCAc                                       <  2:3350752/150‑1 (MQ=255)
                                                                                                              aTGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCAc                                       <  1:1794089/150‑1 (MQ=255)
                                                                                                                     aGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAg                                >  2:2627717/1‑150 (MQ=255)
                                                                                                                     aGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAg                                >  1:2593708/1‑150 (MQ=255)
                                                                                                                       gggAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGt                              <  1:1107212/150‑1 (MQ=255)
                                                                                                                        ggAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTc                             >  1:1887112/1‑150 (MQ=255)
                                                                                                                        ggAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTc                             >  1:3035939/1‑150 (MQ=255)
                                                                                                                         gAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCa                            <  1:2139974/150‑1 (MQ=255)
                                                                                                                          aaaaGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAg                           >  2:3594621/1‑150 (MQ=255)
                                                                                                                           aaaGCCGACCACTTTAACATTGAAATATGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAgg                          <  2:1109428/150‑1 (MQ=255)
                                                                                                                            aaGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGgc                         >  2:1698654/1‑150 (MQ=255)
                                                                                                                            aaGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGgc                         <  2:764576/150‑1 (MQ=255)
                                                                                                                               ccGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGGcgcg                      >  1:445816/1‑150 (MQ=255)
                                                                                                                                     aCTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGGCGCGGTCTGt                >  1:3975025/1‑150 (MQ=255)
                                                                                                                                     aCTTTAACATTGAAAGATGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGGCGCGGTCTGt                <  2:3132666/150‑1 (MQ=255)
                                                                                                                                          aaCATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGGCGCGGTCTGTAGGCt           <  2:1464562/150‑1 (MQ=255)
                                                                                                                                          aaCATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGGCGCGGTCTGTAGGCt           <  2:1327153/150‑1 (MQ=255)
                                                                                                                                          aaCATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGGCGCGGTCTGTAGGCt           >  1:1645095/1‑150 (MQ=255)
                                                                                                                                          aaCATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGGCGCGGTCTGTAGGCt           >  1:3521556/1‑150 (MQ=255)
                                                                                                                                          aaCATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGGCGCGGTCTGTAGGCt           <  1:415108/150‑1 (MQ=255)
                                                                                                                                           aCATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGGCGCGGTCTGTAGGCTg          <  2:1912947/150‑1 (MQ=255)
                                                                                                                                            cATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGGCGCGGTCTGTAGGCTGa         <  1:3708609/150‑1 (MQ=255)
                                                                                                                                             aTTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGGCGCGGTCTGTAGGCTGAt        <  1:3256017/150‑1 (MQ=255)
                                                                                                                                             aTTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGGCGCGGTCTGTAGGCTGAt        >  1:2614256/1‑150 (MQ=255)
                                                                                                                                             aTTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGGCGCGGTCTGTAGGCTGAt        >  1:1000235/1‑150 (MQ=255)
                                                                                                                                             aTTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGGCGCGGTCTGTAGGCTGAt        >  2:54952/1‑150 (MQ=255)
                                                                                                                                               tGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGGCGCGGTCTGTAGGCTGATaa      >  1:1896756/1‑150 (MQ=255)
                                                                                                                                               tGAAATATGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGGCGCGGTCTGTAGGCTGATaa      >  2:2285046/1‑150 (MQ=255)
                                                                                                                                                gAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGGCGCGGTCTGTAGGCTGATAAg     <  1:496385/150‑1 (MQ=255)
                                                                                                                                                  aaTGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGGCGCGGTCTGTAGGCTGATAAGAc   <  1:1672954/150‑1 (MQ=255)
                                                                                                                                                  aaTGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGGCGCGGTCTGTAGGCTGATAAGAc   >  2:3118966/1‑150 (MQ=255)
                                                                                                                                                  aaTGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGGCGCGGTCTGTAGGCTGATAAGAc   >  2:3117807/1‑150 (MQ=255)
                                                                                                                                                  aaTGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGGCGCGGTCTGTAGGCTGATAAGAc   <  1:1673074/150‑1 (MQ=255)
                                                                                                                                                   aTGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGGCGCGGTCTGTAGGCTGATAAGACg  >  2:3779699/1‑150 (MQ=255)
                                                                                                                                                   aTGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGGCGCGGTCTGTAGGCTGATAAGACg  >  2:679270/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
AGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGGCGCGGTCTGTAGGCTGATAAGACG  >  CP009273/3835005‑3835301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A