Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F181 I0 R1 1262 323.1 10297870 97.5% 10040423 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,835,154 G→A 30.3% intergenic (‑55/+156) nepI ← / ← yicN putative transporter/DUF1198 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,835,1540GA30.3% 477.7 / 282.6 287intergenic (‑55/+156)nepI/yicNputative transporter/DUF1198 family protein
Reads supporting (aligned to +/- strand):  ref base G (84/116);  new base A (38/49);  total (122/165)
Fisher's exact test for biased strand distribution p-value = 7.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 302 total aligned reads displayed.

AGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGGCGCGGTCTGTAGGCTGATAAGACGAA  >  CP009273/3835005‑3835303
                                                                                                                                                     |                                                                                                                                                     
aGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAAtg                                                                                                                                                       >  2:2552348/1‑150 (MQ=255)
aGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAAtg                                                                                                                                                       <  1:2352108/150‑1 (MQ=255)
  aCAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAAtgtg                                                                                                                                                     >  1:3335721/1‑150 (MQ=255)
  aCAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAAtatg                                                                                                                                                     >  1:3866853/1‑150 (MQ=255)
  aCAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAAtatg                                                                                                                                                     >  1:546025/1‑150 (MQ=255)
   cAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAAtatgt                                                                                                                                                    <  2:1492600/150‑1 (MQ=255)
    aGGCGACACAAAACGCCACCGAGAAAACGGCTGACCCGTTCGGTCGGGTGAAGGCAACCGCGCCGCGGGTTTCGGCAATAAATTCACTCATGGTGATACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAAtgtgtg                                                                                                                                                   >  2:4277781/1‑150 (MQ=255)
     ggCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGa                                                                                                                                                  <  1:1006749/150‑1 (MQ=255)
      gCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGAc                                                                                                                                                 >  2:1739144/1‑150 (MQ=255)
         acacAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCa                                                                                                                                              <  2:4485845/150‑1 (MQ=255)
                  gCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGtt                                                                                                                                     <  1:1256596/150‑1 (MQ=255)
                    cACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATATGTGACGCATTTAACGtttt                                                                                                                                   <  1:3562299/150‑1 (MQ=255)
                     aCCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATATGTGACGCATTTAACGttttt                                                                                                                                  >  1:4602068/1‑150 (MQ=255)
                        gagaAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATATGTGACGCATTTAACGTTTTTGCa                                                                                                                               <  2:1915033/150‑1 (MQ=255)
                          gaAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAAc                                                                                                                             <  1:45106/150‑1 (MQ=255)
                           aacaCGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACt                                                                                                                            <  2:3703846/147‑1 (MQ=255)
                           aaaaCGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACt                                                                                                                            <  2:3138504/150‑1 (MQ=255)
                           aaaaCGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACt                                                                                                                            <  2:2966889/150‑1 (MQ=255)
                            aaaCGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACtt                                                                                                                           <  2:4136520/150‑1 (MQ=255)
                             aaCGGCTGACCCGTTCGGTCGGGTGATGGCAGCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACttt                                                                                                                          <  1:875982/150‑1 (MQ=255)
                              aCGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATATGTGACGCATTTAACGTTTTTGCAACtttt                                                                                                                         <  2:1423128/150‑1 (MQ=255)
                                  cTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACgg                                                                                                                     <  1:2996934/150‑1 (MQ=255)
                                    gACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGgtg                                                                                                                   <  2:1621702/150‑1 (MQ=255)
                                    gACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATATGTGACGCATTTAACGTTTTTGCAACTTTTACGgtg                                                                                                                   <  1:3358606/150‑1 (MQ=255)
                                    gACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATATGTGACGCATTTAACGTTTTTGCAACTTTTACGgtg                                                                                                                   >  2:2474441/1‑150 (MQ=255)
                                       cAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTg                                                                                                                >  1:4318874/1‑150 (MQ=255)
                                       cAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTg                                                                                                                >  2:2847501/1‑150 (MQ=255)
                                         gTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCCTGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCt                                                                                                              <  1:2797243/150‑1 (MQ=255)
                                         gTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCAGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATATGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCt                                                                                                              >  1:2205189/1‑150 (MQ=255)
                                          ttCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCtt                                                                                                             <  1:4741623/150‑1 (MQ=255)
                                          ttCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGGTTTTGCAACTTTTACGGTGTTGCtt                                                                                                             >  2:629329/1‑150 (MQ=255)
                                           tcggtcggGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATATGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTg                                                                                                            >  2:2631258/1‑150 (MQ=255)
                                            cggtcggGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGgtgt                                                                                                                  <  2:4719580/143‑1 (MQ=255)
                                            cggtcggGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGgtgt                                                                                                                  >  1:4719490/1‑143 (MQ=255)
                                              gtcggGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGCGAAAAGCCGACCACATTAACCTTGAAATATGTGACGCATATAACGAAATTGCAACTATAACGGTCTTGCGTGAGc                                                                                                         >  1:5054198/1‑150 (MQ=255)
                                               tcggGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATATGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCt                                                                                                        <  1:4835351/150‑1 (MQ=255)
                                                  ggTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGa                                                                                                     <  1:2617857/150‑1 (MQ=255)
                                                   gTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAg                                                                                                    >  1:3975694/1‑150 (MQ=255)
                                                    tGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGt                                                                                                   <  1:1646988/150‑1 (MQ=255)
                                                    tGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGt                                                                                                   >  2:4565724/1‑150 (MQ=255)
                                                    tGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGt                                                                                                   <  1:4208831/150‑1 (MQ=255)
                                                     gATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATATGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGtt                                                                                                  <  2:4543058/150‑1 (MQ=255)
                                                     gATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATATGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGtt                                                                                                  >  2:3025407/1‑150 (MQ=255)
                                                     gATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATATGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGtt                                                                                                  <  1:4404633/150‑1 (MQ=255)
                                                        ggCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATATGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAg                                                                                               >  2:1444251/1‑150 (MQ=255)
                                                         gCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGa                                                                                              <  2:2734579/150‑1 (MQ=255)
                                                          cATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGAt                                                                                             <  2:3493147/150‑1 (MQ=255)
                                                          cATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGAt                                                                                             <  2:504224/150‑1 (MQ=255)
                                                          cATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGAt                                                                                             >  2:5032689/1‑150 (MQ=255)
                                                          cATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGGGTGACGCATTTAACGTTTTTGCACCTTTTACGGTGTTGCTTGAGCTGGAGTTAAGAt                                                                                             >  1:360594/1‑150 (MQ=255)
                                                             ccgcgccgcgGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGa                                                                                          <  1:4558852/150‑1 (MQ=255)
                                                                cgccgcgGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAAc                                                                                       <  1:1106677/150‑1 (MQ=255)
                                                                cgccgcgGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAAc                                                                                       >  2:1183032/1‑150 (MQ=255)
                                                                 gccgcgGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACg                                                                                      <  1:3526679/150‑1 (MQ=255)
                                                                 gccgcgGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATATGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACg                                                                                      <  1:3720105/150‑1 (MQ=255)
                                                                  ccgcgGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACgg                                                                                     <  1:1890974/150‑1 (MQ=255)
                                                                  ccgcgGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACgg                                                                                     <  1:3203660/150‑1 (MQ=255)
                                                                   cgcgGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGa                                                                                    >  2:3373057/1‑150 (MQ=255)
                                                                     cgGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAAc                                                                                  >  2:656213/1‑150 (MQ=255)
                                                                      ggTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACa                                                                                 >  2:3560153/1‑150 (MQ=255)
                                                                       gTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAg                                                                                <  2:1138933/150‑1 (MQ=255)
                                                                        ttttCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAgg                                                                               >  1:787261/1‑150 (MQ=255)
                                                                        ttttCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATATGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAgg                                                                               >  1:2774530/1‑150 (MQ=255)
                                                                         tttCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAggg                                                                              <  2:603870/150‑1 (MQ=255)
                                                                          ttCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGc                                                                             <  1:4889275/150‑1 (MQ=255)
                                                                          ttCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGc                                                                             <  1:3463331/150‑1 (MQ=255)
                                                                               cAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGa                                                                        <  1:3927580/150‑1 (MQ=255)
                                                                                  tAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTa                                                                     <  2:4816540/150‑1 (MQ=255)
                                                                                  tAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATATGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTa                                                                     <  1:4045779/150‑1 (MQ=255)
                                                                                   aaaTTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTAc                                                                    <  2:2417737/150‑1 (MQ=255)
                                                                                      ttCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCgg                                                                 >  2:3858982/1‑150 (MQ=255)
                                                                                        cACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGAt                                                               <  1:3391786/150‑1 (MQ=255)
                                                                                         aCTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATATGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATg                                                              <  2:1893389/150‑1 (MQ=255)
                                                                                          cTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATgc                                                             <  2:4589877/150‑1 (MQ=255)
                                                                                              tGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATgcggcg                                                         <  1:2091309/150‑1 (MQ=255)
                                                                                              tGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATgcggcg                                                         <  1:1420938/150‑1 (MQ=255)
                                                                                               ggTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGt                                                        <  1:814044/150‑1 (MQ=255)
                                                                                                   ttACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAAc                                                    >  2:1864657/1‑150 (MQ=255)
                                                                                                            gCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATcc                                           <  2:4315558/150‑1 (MQ=255)
                                                                                                            gCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATcc                                           >  1:677836/1‑150 (MQ=255)
                                                                                                            gCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATcc                                           >  2:4094780/1‑150 (MQ=255)
                                                                                                               tGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATATGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCAcc                                        >  1:1973604/1‑150 (MQ=255)
                                                                                                                gTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCAccc                                       <  2:1082729/150‑1 (MQ=255)
                                                                                                                     aGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAg                                  <  2:713039/150‑1 (MQ=255)
                                                                                                                        ggAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTc                               <  2:2314800/150‑1 (MQ=255)
                                                                                                                             aGCCGACCACTTTAACATTGAAATATGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGgcg                          >  2:326263/1‑150 (MQ=255)
                                                                                                                                cGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGGCGCgg                       >  2:2715377/1‑150 (MQ=255)
                                                                                                                                   ccACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGGCGCGGTCt                    <  1:3054790/150‑1 (MQ=255)
                                                                                                                                   ccACTTTAACATTGAAATATGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGGCGCGGTCt                    <  1:2875003/150‑1 (MQ=255)
                                                                                                                                   ccACTTTAACATTGAAATATGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGGCGCGGTCt                    <  1:2452287/150‑1 (MQ=255)
                                                                                                                                    cACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGGCGCGGTCTg                   >  2:3516963/1‑150 (MQ=255)
                                                                                                                                       tttAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGGCGCGGTCTGTAg                <  1:1739144/150‑1 (MQ=255)
                                                                                                                                       tttAACATTGAAATATGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGGCGCGGTCTGTAg                >  1:1826648/1‑150 (MQ=255)
                                                                                                                                        ttAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGGCGCGGTCTGTAgg               >  2:3590268/1‑150 (MQ=255)
                                                                                                                                        ttAACATTGAAATATGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGGCGCGGTCTGTAgg               <  2:4060529/150‑1 (MQ=255)
                                                                                                                                           aCATTGAAATATGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGGCGCGGTCTGTAGGCTg            >  2:804378/1‑150 (MQ=255)
                                                                                                                                               tGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGGCGCGGTCTGTAGGCTGATaa        >  1:3733472/1‑150 (MQ=255)
                                                                                                                                               tGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGGCGCGGTCTGTAGGCTGATaa        <  2:2793653/150‑1 (MQ=255)
                                                                                                                                                gAAATATGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGGCGCGGTCTGTAGGCTGATAAg       >  1:1324801/1‑150 (MQ=255)
                                                                                                                                                gAAATATGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGGCGCGGTCTGTAGGCTGATAAg       <  2:853796/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTACCCGTGGCATGTTTCAGGGGAAAAGCCGACCACTTTAACATTGAAATGTGTGACGCATTTAACGTTTTTGCAACTTTTACGGTGTTGCTTGAGCTGGAGTTAAGATCGAAACGGAACAGGGCAAAGATTACCGGATGCGGCGTGAACGTCTTATCCACCCGACAGGTCAGGCGCGGTCTGTAGGCTGATAAGACGAA  >  CP009273/3835005‑3835303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A