Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,950,395 C→A 11.2% E262D (GAG→GAT)  ilvY ← transcriptional activator of ilvC; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,950,3950CA11.2% 927.1 / 63.4 267E262D (GAG→GAT) ilvYtranscriptional activator of ilvC; autorepressor
Reads supporting (aligned to +/- strand):  ref base C (142/95);  new base A (15/15);  total (157/110)
Fisher's exact test for biased strand distribution p-value = 3.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

Only 100 of 277 total aligned reads displayed.

CTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTT  >  CP009273/3950246‑3950544
                                                                                                                                                     |                                                                                                                                                     
ccgATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTgcgc                                                                                                                                                       >  1:3065683/3‑150 (MQ=255)
    tAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGATCTa                                                                                                                                                   <  2:83063/150‑1 (MQ=255)
        gcttcgcttATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAat                                                                                                                                               >  1:781223/1‑150 (MQ=255)
        gcttcgcttATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAat                                                                                                                                               >  1:2413222/1‑150 (MQ=255)
            cgcttATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAatcatc                                                                                                                                           <  2:2575233/150‑1 (MQ=255)
            cgcttATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAatcatc                                                                                                                                           <  2:2521154/150‑1 (MQ=255)
             gcttATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGATCTAAAAtcatca                                                                                                                                          >  2:1105415/1‑150 (MQ=255)
               ttATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCAcg                                                                                                                                        >  2:183065/1‑150 (MQ=255)
                tATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCAcgc                                                                                                                                       >  1:4126374/1‑150 (MQ=255)
                 aTCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCAcgcg                                                                                                                                      >  2:3905108/1‑150 (MQ=255)
                   cAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGt                                                                                                                                    >  2:2247673/1‑150 (MQ=255)
                   cAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGt                                                                                                                                    >  2:3186066/1‑150 (MQ=255)
                      gCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACCCGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTAc                                                                                                                                 <  2:3020262/150‑1 (MQ=255)
                      gCCTACCCGCGCGACAACGGCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTAc                                                                                                                                 >  2:2283462/1‑150 (MQ=255)
                        cTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGc                                                                                                                               <  2:1775260/150‑1 (MQ=255)
                         tACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCa                                                                                                                              <  1:3910777/150‑1 (MQ=255)
                           cccGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCAcc                                                                                                                            >  2:653579/1‑150 (MQ=255)
                            ccGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCg                                                                                                                           >  1:3460344/1‑150 (MQ=255)
                            ccGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCg                                                                                                                           >  1:2095742/1‑150 (MQ=255)
                            ccGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCg                                                                                                                           <  2:2428600/150‑1 (MQ=255)
                                 cgACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCg                                                                                                                      <  2:3124051/150‑1 (MQ=255)
                                 cgACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCg                                                                                                                      >  1:1499560/1‑150 (MQ=255)
                                     aaCGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGc                                                                                                                  >  2:3925697/1‑150 (MQ=255)
                                     aaCGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGc                                                                                                                  >  2:4332217/1‑150 (MQ=255)
                                      aCGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCt                                                                                                                 >  1:3946032/1‑150 (MQ=255)
                                      aCGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCt                                                                                                                 <  1:2063943/150‑1 (MQ=255)
                                        gTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCTGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGt                                                                                                               >  1:247197/1‑150 (MQ=255)
                                        gTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGt                                                                                                               <  1:3349331/150‑1 (MQ=255)
                                         tCATTTGTGGTTCGGCAAACTCGTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCATTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGtt                                                                                                              <  2:2598339/150‑1 (MQ=255)
                                          cATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACCCGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGttt                                                                                                             <  2:2774496/150‑1 (MQ=255)
                                          cATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGttt                                                                                                             >  2:1680152/1‑150 (MQ=255)
                                          cATTTGTGGTTCGGCAAAATCTTCAAGAATGCCTCAATTTGGGGCACATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTCCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGttt                                                                                                             <  2:4410738/150‑1 (MQ=255)
                                           aTTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGATCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGtttt                                                                                                            >  1:3800574/1‑150 (MQ=255)
                                               gtgGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGATCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAg                                                                                                        <  1:3566277/150‑1 (MQ=255)
                                                 ggTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGca                                                                                                      >  2:3636214/1‑150 (MQ=255)
                                                 ggTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGca                                                                                                      >  1:2506951/1‑150 (MQ=255)
                                                 ggTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGca                                                                                                      >  2:3992596/1‑150 (MQ=255)
                                                         aaaaTCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTcc                                                                                              >  1:1861696/1‑150 (MQ=255)
                                                         aaaaTCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTcc                                                                                              >  1:2046193/1‑150 (MQ=255)
                                                           aaTCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCCCGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCgg                                                                                            <  1:3299660/150‑1 (MQ=255)
                                                           aaTCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCgg                                                                                            >  1:3679726/1‑150 (MQ=255)
                                                           aaTCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGATCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCgg                                                                                            >  1:611019/1‑150 (MQ=255)
                                                              cTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGcaa                                                                                         <  1:2646196/150‑1 (MQ=255)
                                                              cTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGcaa                                                                                         >  1:3461479/1‑150 (MQ=255)
                                                                  cAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAAt                                                                                     >  1:1067804/1‑150 (MQ=255)
                                                                    gAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATgc                                                                                   <  2:3588602/150‑1 (MQ=255)
                                                                        gCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATgccacg                                                                               <  1:1884760/150‑1 (MQ=255)
                                                                        gCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATgccacg                                                                               <  2:2637144/150‑1 (MQ=255)
                                                                           tCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATgccacgcca                                                                            >  1:822371/1‑150 (MQ=255)
                                                                            cAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATgccacgccac                                                                           >  2:4516719/1‑150 (MQ=255)
                                                                            cAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATgccacgccac                                                                           <  2:973174/150‑1 (MQ=255)
                                                                             aaTTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCcaca                                                                          <  2:3789580/150‑1 (MQ=255)
                                                                             aaTTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCcaca                                                                          <  2:4275870/150‑1 (MQ=255)
                                                                             aaTTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGATCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCcaca                                                                          <  2:3404835/150‑1 (MQ=255)
                                                                              aTTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAg                                                                         <  1:321639/150‑1 (MQ=255)
                                                                                tAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGcc                                                                       >  1:3973434/1‑150 (MQ=255)
                                                                                 aGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCg                                                                      >  1:1549742/1‑150 (MQ=255)
                                                                                   cGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGATCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAg                                                                    >  1:704467/1‑150 (MQ=255)
                                                                                    ggCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCACACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCTGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGt                                                                   <  1:3640685/150‑1 (MQ=255)
                                                                                       tCATGGAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGcc                                                                <  1:2390986/150‑1 (MQ=255)
                                                                                           gTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGATCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGccacca                                                            <  2:731596/150‑1 (MQ=255)
                                                                                            tAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCAt                                                           <  2:2086034/150‑1 (MQ=255)
                                                                                            tAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCAt                                                           >  2:4044448/1‑150 (MQ=255)
                                                                                             aGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATc                                                          >  1:1733746/1‑150 (MQ=255)
                                                                                             aGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATc                                                          >  1:4379654/1‑150 (MQ=255)
                                                                                              gCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCg                                                         <  2:4114449/150‑1 (MQ=255)
                                                                                                cGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGAt                                                       >  2:2065585/1‑150 (MQ=255)
                                                                                                cGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGAt                                                       <  2:4096913/150‑1 (MQ=255)
                                                                                                cGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGAt                                                       >  2:607471/1‑150 (MQ=255)
                                                                                                    ttttCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCa                                                   <  1:2905436/150‑1 (MQ=255)
                                                                                                      ttCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATc                                                 >  1:4527870/1‑150 (MQ=255)
                                                                                                       tCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCg                                                <  1:1181586/150‑1 (MQ=255)
                                                                                                       tCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCAACg                                                >  1:3531737/1‑150 (MQ=255)
                                                                                                           cgcACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTc                                            <  1:4041244/150‑1 (MQ=255)
                                                                                                           cgcACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTc                                            >  2:2121890/1‑150 (MQ=255)
                                                                                                           cgcACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTc                                            <  1:1337381/150‑1 (MQ=255)
                                                                                                           cgcACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTc                                            <  2:3704050/150‑1 (MQ=255)
                                                                                                                acacGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGATCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGcc                                       >  2:2866839/1‑150 (MQ=255)
                                                                                                                        aGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAAcc                               <  1:2557162/150‑1 (MQ=255)
                                                                                                                        aGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAAcc                               >  1:304651/1‑150 (MQ=255)
                                                                                                                        aGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACGTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCAACGATGCCATCGCTTCATGCCCGCCCAcc                               >  1:1100906/1‑150 (MQ=255)
                                                                                                                        aGCTCAAACGGCGTTTTCTCATCGCTGCGATCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAAcc                               <  1:1709392/150‑1 (MQ=255)
                                                                                                                             aaaCGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGc                          >  2:1699840/1‑150 (MQ=255)
                                                                                                                             aaaCGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGc                          >  1:643133/1‑150 (MQ=255)
                                                                                                                              aaCGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCg                         >  2:3086968/1‑150 (MQ=255)
                                                                                                                                cGGCGTTTTCTCCTCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTa                       <  1:915443/150‑1 (MQ=255)
                                                                                                                                cGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTa                       >  2:1327168/1‑150 (MQ=255)
                                                                                                                                   cGTTTTCTCATCGCTGCGATCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAat                    >  2:3210018/1‑150 (MQ=255)
                                                                                                                                    gTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAatc                   >  2:1474267/1‑150 (MQ=255)
                                                                                                                                        tctcATCGCTGCGCTCTATAATCATCACCCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCg               >  1:4433576/1‑150 (MQ=255)
                                                                                                                                           cATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGt            <  2:3735033/150‑1 (MQ=255)
                                                                                                                                           cATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGt            <  2:3735193/150‑1 (MQ=255)
                                                                                                                                             tCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTa          <  2:2617513/150‑1 (MQ=255)
                                                                                                                                             tCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTa          <  2:3506220/150‑1 (MQ=255)
                                                                                                                                                cTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTACATCATCGGGTTACTg       >  1:531265/1‑150 (MQ=255)
                                                                                                                                                  gcgcTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGAt     <  1:2936633/150‑1 (MQ=255)
                                                                                                                                                   cgcTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGAtt    <  1:3993062/150‑1 (MQ=255)
                                                                                                                                                    gcTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGAttt   <  2:2563308/150‑1 (MQ=255)
                                                                                                                                                    gcTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGAttt   >  1:4329814/1‑150 (MQ=255)
                                                                                                                                                     ctctAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGAtttt  >  2:2172538/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTT  >  CP009273/3950246‑3950544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A