Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F159 I0 R1 879 305.9 9588012 96.6% 9262019 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,950,395 C→A 30.6% E262D (GAG→GAT)  ilvY ← transcriptional activator of ilvC; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,950,3950CA30.6% 532.0 / 232.7 277E262D (GAG→GAT) ilvYtranscriptional activator of ilvC; autorepressor
Reads supporting (aligned to +/- strand):  ref base C (96/96);  new base A (47/38);  total (143/134)
Fisher's exact test for biased strand distribution p-value = 4.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 289 total aligned reads displayed.

CTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTT  >  CP009273/3950246‑3950544
                                                                                                                                                     |                                                                                                                                                     
   aTAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGctct                                                                                                                                                    >  1:1123598/1‑150 (MQ=255)
   aTAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGctct                                                                                                                                                    <  1:1356074/150‑1 (MQ=255)
   aTAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGctct                                                                                                                                                    >  1:1722101/1‑150 (MQ=255)
   aTAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGctct                                                                                                                                                    >  1:2172587/1‑150 (MQ=255)
    tAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTa                                                                                                                                                   <  2:1406994/150‑1 (MQ=255)
    tAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTa                                                                                                                                                   <  1:1506085/150‑1 (MQ=255)
       cgcttcgcttATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTaaaa                                                                                                                                                >  1:1919215/1‑150 (MQ=255)
             gcttATCAGGCCTACCCGCGCGACACCGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAAtcatca                                                                                                                                          <  2:4353143/150‑1 (MQ=255)
             gcttATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGATCTAAAAtcatca                                                                                                                                          <  1:1041795/150‑1 (MQ=255)
               ttATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCAcg                                                                                                                                        <  2:1220825/150‑1 (MQ=255)
               ttATCAGGCATACCCGCGCGACAACGTCATTTTTGGTTCGGCAAAATCTTCCAGAATGCCTAAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCAcg                                                                                                                                        <  1:3333304/150‑1 (MQ=255)
                 aTCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCAcgcg                                                                                                                                      <  2:1266301/150‑1 (MQ=255)
                 aTCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGATCTAAAATCATCAcgcg                                                                                                                                      <  2:1666023/150‑1 (MQ=255)
                   cAGGCCTACCCGCGCGACAACGTAATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGt                                                                                                                                    <  1:417096/150‑1 (MQ=255)
                    aGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGtt                                                                                                                                   <  1:1002484/150‑1 (MQ=255)
                    aGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGtt                                                                                                                                   >  1:2775299/1‑150 (MQ=255)
                       ccTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACg                                                                                                                                <  2:1491943/150‑1 (MQ=255)
                        cTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGc                                                                                                                               >  2:4637643/1‑150 (MQ=255)
                            ccGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCg                                                                                                                           <  1:1303195/150‑1 (MQ=255)
                             cgcgcgACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGATCTAAAATCATCACGCGGTTACGCACCgg                                                                                                                          >  2:72263/1‑150 (MQ=255)
                              gcgcgACAACGTCATTTGTGGGTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGATCTAAAATCATCACGCGGTTACGCACCGGt                                                                                                                         <  1:4240103/150‑1 (MQ=255)
                                    cAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCgggg                                                                                                                   <  2:1212557/150‑1 (MQ=255)
                                     aaCGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGATCTAAAATCATCACGCGGTTACGCACCGGTTAGGGGc                                                                                                                  >  2:104660/1‑150 (MQ=255)
                                      aCGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGATCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCt                                                                                                                 <  2:195559/150‑1 (MQ=255)
                                       cGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTg                                                                                                                >  2:2439351/1‑150 (MQ=255)
                                       cGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTATGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGATCTAAAATAATCACGCGGTTACGCACCGGTTCGGGGCTg                                                                                                                >  1:2664046/1‑150 (MQ=255)
                                         tCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGtt                                                                                                              >  1:80617/1‑150 (MQ=255)
                                          cATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCATTTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGGTCGGGGCTGttt                                                                                                             <  1:3157053/150‑1 (MQ=255)
                                            tttGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACAGGTTCGGGGCTGTTTTc                                                                                                           >  2:832648/1‑150 (MQ=255)
                                                  gTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGcac                                                                                                     <  2:2142959/150‑1 (MQ=255)
                                                  gTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGATCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGcac                                                                                                     <  2:439291/150‑1 (MQ=255)
                                                  gTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGATCTAAAATCATCACGCGGTTACGCACCGGGTCGGGGCTGTTTTCCAGcac                                                                                                     >  1:712953/1‑150 (MQ=255)
                                                       gCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCACACGGCGGTTTCTCATCGCAGCGCGCGAAAATCAACACCCGGTTACGCACCCGGTCGGGGCTGTTTTCCCGcaccacgt                                                                                                >  1:4041947/1‑148 (MQ=255)
                                                        cAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTc                                                                                               >  1:2371560/1‑150 (MQ=255)
                                                        cAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCAGCGCACACACGCCCAGCACAAACGGCGTTTTCTCATCGCTGCGATCAAAAATCAAAACCCCGTTACCCACCCGGTCGGGGCTGTTTTCCAGCACCACATc                                                                                               >  1:540055/1‑150 (MQ=255)
                                                           aaTCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCgg                                                                                            >  2:927566/1‑150 (MQ=255)
                                                           aaTCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCgg                                                                                            >  2:2266411/1‑150 (MQ=255)
                                                           aaTCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCAACACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCgg                                                                                            >  1:2981121/1‑150 (MQ=255)
                                                            aTCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGATCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGc                                                                                           >  1:3055486/1‑150 (MQ=255)
                                                            aTCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGATCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGc                                                                                           >  1:4792105/1‑150 (MQ=255)
                                                               ttCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGATCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGcaac                                                                                        >  1:1738428/1‑150 (MQ=255)
                                                                tCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGATCAAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTACGGcaaca                                                                                       >  1:592885/1‑150 (MQ=255)
                                                                  cAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAAt                                                                                     <  2:1876229/150‑1 (MQ=255)
                                                                   aGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATg                                                                                    >  1:4651268/1‑150 (MQ=255)
                                                                    gAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGATCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATgc                                                                                   <  1:3224361/150‑1 (MQ=255)
                                                                     aaTGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGATCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATgcc                                                                                  >  2:3080076/1‑150 (MQ=255)
                                                                       tGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATgccac                                                                                <  2:373306/150‑1 (MQ=255)
                                                                         ccTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATgccacgc                                                                              >  1:2572964/1‑150 (MQ=255)
                                                                          cTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGATCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATgccacgcc                                                                             >  1:2639501/1‑150 (MQ=255)
                                                                            cAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATgccacgccac                                                                           >  1:4138318/1‑150 (MQ=255)
                                                                                tAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGATCTAACATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGcc                                                                       <  2:2628886/150‑1 (MQ=255)
                                                                                tAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGATCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGcc                                                                       <  2:2569316/150‑1 (MQ=255)
                                                                                tAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGATCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGcc                                                                       <  2:178820/150‑1 (MQ=255)
                                                                                 aGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCCCGCCACAGCCg                                                                      >  2:3596081/1‑150 (MQ=255)
                                                                                     gCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTg                                                                  <  1:3832019/150‑1 (MQ=255)
                                                                                     gCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGATCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTg                                                                  >  2:2499438/1‑150 (MQ=255)
                                                                                      cTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGATCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGc                                                                 <  1:4516313/150‑1 (MQ=255)
                                                                                      cTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGATCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGc                                                                 >  2:4151231/1‑150 (MQ=255)
                                                                                         aTGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGccac                                                              >  2:4323406/1‑150 (MQ=255)
                                                                                          tGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGccacc                                                             <  1:4446427/150‑1 (MQ=255)
                                                                                                cGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGAt                                                       >  1:3715988/1‑150 (MQ=255)
                                                                                                 gCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGATCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATa                                                      <  1:1972771/150‑1 (MQ=255)
                                                                                                  cTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATAc                                                     >  1:2341653/1‑150 (MQ=255)
                                                                                                  cTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGATCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATAc                                                     <  2:1092078/150‑1 (MQ=255)
                                                                                                        cTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGc                                               >  2:2914351/1‑150 (MQ=255)
                                                                                                         tGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGATCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCt                                              <  2:154905/150‑1 (MQ=255)
                                                                                                              acacacGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATGCCATCGCTTCATg                                         <  1:4316209/150‑1 (MQ=255)
                                                                                                              acacacGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATg                                         <  2:253834/150‑1 (MQ=255)
                                                                                                              acacacGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATg                                         <  1:2914351/150‑1 (MQ=255)
                                                                                                              acacacGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATg                                         <  2:1167282/150‑1 (MQ=255)
                                                                                                              acacacGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATg                                         <  1:3546068/150‑1 (MQ=255)
                                                                                                                  acGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGATCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCg                                     <  1:2424485/150‑1 (MQ=255)
                                                                                                                    gCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGcc                                   >  1:2578279/1‑150 (MQ=255)
                                                                                                                     cccAGCTCAAACGGCGTTTTCTCATCGCTGCGATCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCa                                  <  1:1020654/150‑1 (MQ=255)
                                                                                                                      ccAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCaa                                 >  2:1602913/1‑150 (MQ=255)
                                                                                                                         gCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCg                              >  1:3924512/1‑150 (MQ=255)
                                                                                                                           tCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTg                            <  2:546573/150‑1 (MQ=255)
                                                                                                                           tCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTg                            >  1:3430492/1‑150 (MQ=255)
                                                                                                                           tCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTg                            >  2:3465715/1‑150 (MQ=255)
                                                                                                                            cAAACGGCGTTTTCTCATCGCTGCGATCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTgg                           >  2:351232/1‑150 (MQ=255)
                                                                                                                                cGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTa                       <  1:687064/150‑1 (MQ=255)
                                                                                                                                cGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTa                       <  2:3976737/150‑1 (MQ=255)
                                                                                                                                   cGTTTTCTCATCGCTGCGATCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAat                    >  2:2738037/1‑150 (MQ=255)
                                                                                                                                    gTTTTCTCATCGCTGCGATCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAatc                   >  1:1446128/1‑150 (MQ=255)
                                                                                                                                     ttttCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAatca                  <  2:1722101/150‑1 (MQ=255)
                                                                                                                                       ttCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAatcatc                >  1:2452666/1‑150 (MQ=255)
                                                                                                                                       ttCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAatcatc                <  2:4041942/150‑1 (MQ=255)
                                                                                                                                       ttCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAatcatc                <  2:2453034/150‑1 (MQ=255)
                                                                                                                                        tctcATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCg               <  1:880033/150‑1 (MQ=255)
                                                                                                                                         ctcATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCgg              >  2:4736820/1‑150 (MQ=255)
                                                                                                                                         ctcATCGCTGCGATCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCgg              >  1:2552062/1‑150 (MQ=255)
                                                                                                                                          tcATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCggg             >  1:706995/1‑150 (MQ=255)
                                                                                                                                           cATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCACCAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGt            >  2:3832885/1‑150 (MQ=255)
                                                                                                                                            aTCGCTGCGATCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGtt           >  2:45549/1‑150 (MQ=255)
                                                                                                                                             tCGCTGCGATCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTa          <  1:124490/150‑1 (MQ=255)
                                                                                                                                               gCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACt        <  2:1123598/150‑1 (MQ=255)
                                                                                                                                               gCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACt        <  2:1919215/150‑1 (MQ=255)
                                                                                                                                                cTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTg       <  1:44643/150‑1 (MQ=255)
                                                                                                                                                 tGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGa      <  2:2943973/150‑1 (MQ=255)
                                                                                                                                                    gcTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGAttt   <  1:3784493/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTGCGCTCTAAAATCATCACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTT  >  CP009273/3950246‑3950544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A