Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 142,460 T→A 89.5% F298Y (TTC→TAC)  yadE → putative polysaccharide deacetylase lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273142,4600TA89.5% 780.3 / 68.5 238F298Y (TTC→TAC) yadEputative polysaccharide deacetylase lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (12/13);  new base A (95/118);  total (107/131)
Fisher's exact test for biased strand distribution p-value = 8.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-01

Only 100 of 239 total aligned reads displayed.

CGTATCCTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTTCGTA  >  CP009273/142313‑142607
                                                                                                                                                   |                                                                                                                                                   
cGTATCCTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTAcc                                                                                                                                                   >  2:4303332/1‑150 (MQ=255)
cGTATCCTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTAcc                                                                                                                                                   >  2:1458547/1‑150 (MQ=255)
cGTATCCTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTAcc                                                                                                                                                   <  1:3769054/150‑1 (MQ=255)
cGTATCCTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTAcc                                                                                                                                                   >  2:1112085/1‑150 (MQ=255)
    tCCTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTc                                                                                                                                               <  1:4387147/150‑1 (MQ=255)
    tCCTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTc                                                                                                                                               <  2:4058575/150‑1 (MQ=255)
     ccTGTTTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTca                                                                                                                                              >  1:1871096/1‑150 (MQ=255)
     ccTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTca                                                                                                                                              >  2:726339/1‑150 (MQ=255)
             gAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCa                                                                                                                                      >  2:2542938/1‑150 (MQ=255)
              aaaCAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCAt                                                                                                                                     <  2:4447850/150‑1 (MQ=255)
                      tGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACATCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCa                                                                                                                             >  1:2422141/1‑150 (MQ=255)
                         cATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATTAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTTCATCg                                                                                                                          >  1:2352774/1‑150 (MQ=255)
                         cATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCg                                                                                                                          >  1:3974649/1‑150 (MQ=255)
                         cATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCg                                                                                                                          <  1:347105/150‑1 (MQ=255)
                          aTGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCgg                                                                                                                         >  2:3167487/1‑150 (MQ=255)
                           tGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCggg                                                                                                                        >  2:2488448/1‑150 (MQ=255)
                           tGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCggg                                                                                                                        >  1:2634164/1‑150 (MQ=255)
                            gAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGt                                                                                                                       <  2:3971902/150‑1 (MQ=255)
                                gCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGAt                                                                                                                   <  2:1305660/150‑1 (MQ=255)
                                 cGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATg                                                                                                                  <  2:2210215/150‑1 (MQ=255)
                                 cGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATg                                                                                                                  <  2:2389753/150‑1 (MQ=255)
                                  gACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTATGAGCTTAACGAAATTCGCGATGTATTTGATTAACAGTCACATACCCATTTTTTGCATCGGGTAGATgg                                                                                                                 >  1:1016583/1‑150 (MQ=255)
                                   aCGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGt                                                                                                                >  1:729673/1‑150 (MQ=255)
                                   aCGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGt                                                                                                                >  1:2993148/1‑150 (MQ=255)
                                     ggCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTa                                                                                                              >  2:2418971/1‑150 (MQ=255)
                                     ggCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTa                                                                                                              <  2:2654997/150‑1 (MQ=255)
                                      gCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTAt                                                                                                             <  1:3298247/150‑1 (MQ=255)
                                      gCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTAt                                                                                                             <  1:1185342/150‑1 (MQ=255)
                                       cGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATc                                                                                                            <  1:1098190/150‑1 (MQ=255)
                                        gTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCg                                                                                                           >  1:3690217/1‑150 (MQ=255)
                                         tttATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGc                                                                                                          >  2:1254381/1‑150 (MQ=255)
                                          ttATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGcc                                                                                                         >  1:167609/1‑150 (MQ=255)
                                             ttGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGAc                                                                                                      <  1:2800919/150‑1 (MQ=255)
                                                 tACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCat                                                                                                  <  2:4274403/150‑1 (MQ=255)
                                                  aCCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCata                                                                                                 <  2:1387812/150‑1 (MQ=255)
                                                      cACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTAc                                                                                             <  2:4289190/150‑1 (MQ=255)
                                                       aCGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACt                                                                                            <  1:4145576/150‑1 (MQ=255)
                                                        cGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTg                                                                                           >  1:933203/1‑150 (MQ=255)
                                                           aTCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGc                                                                                        >  1:3436782/1‑150 (MQ=255)
                                                            tCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGcc                                                                                       <  2:3259969/150‑1 (MQ=255)
                                                             cAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCg                                                                                      >  2:3519874/1‑150 (MQ=255)
                                                                   tCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGa                                                                                >  2:2674998/1‑150 (MQ=255)
                                                                   tCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGa                                                                                >  1:2185849/1‑150 (MQ=255)
                                                                   tCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGa                                                                                >  2:409588/1‑150 (MQ=255)
                                                                      cccGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGca                                                                             <  1:1589563/150‑1 (MQ=255)
                                                                      cccGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGca                                                                             <  1:1308939/150‑1 (MQ=255)
                                                                      cccGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGca                                                                             >  1:536401/1‑150 (MQ=255)
                                                                       ccGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGcac                                                                            <  2:1769740/150‑1 (MQ=255)
                                                                          cAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAat                                                                         <  1:3999547/150‑1 (MQ=255)
                                                                          cAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAat                                                                         <  2:2167876/150‑1 (MQ=255)
                                                                           aGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAata                                                                        >  1:3659268/1‑150 (MQ=255)
                                                                              aGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTc                                                                     <  1:4054996/150‑1 (MQ=255)
                                                                                 ggAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGt                                                                  >  1:3600891/1‑150 (MQ=255)
                                                                                  gAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGtt                                                                 <  1:2858886/150‑1 (MQ=255)
                                                                                  gAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGtt                                                                 <  2:2972233/150‑1 (MQ=255)
                                                                                   aaCCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGttt                                                                <  1:3562403/150‑1 (MQ=255)
                                                                                    aCCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTg                                                               >  2:4210840/1‑150 (MQ=255)
                                                                                    aCCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTg                                                               <  1:3253314/150‑1 (MQ=255)
                                                                                    aCCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTg                                                               <  2:4521523/150‑1 (MQ=255)
                                                                                       cAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGAtt                                                            <  1:1993915/150‑1 (MQ=255)
                                                                                        aaaaTCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGAttt                                                           <  2:1390207/150‑1 (MQ=255)
                                                                                           aTCGCTTCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGc                                                        <  2:655089/150‑1 (MQ=255)
                                                                                              gCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACg                                                     >  1:391169/1‑150 (MQ=255)
                                                                                                tGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGtt                                                   <  2:86336/150‑1 (MQ=255)
                                                                                                  cAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCa                                                 >  1:1317355/1‑150 (MQ=255)
                                                                                                  cAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCa                                                 >  1:2454689/1‑150 (MQ=255)
                                                                                                   aaTTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCAc                                                <  1:2009383/150‑1 (MQ=255)
                                                                                                   aaTTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCAc                                                >  2:71657/1‑150 (MQ=255)
                                                                                                    aTTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCAcg                                               >  2:1921969/1‑150 (MQ=255)
                                                                                                      ttATGAGCGTTTCTGAGCTTAACGAACTTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCAcgcc                                             <  2:2339504/150‑1 (MQ=255)
                                                                                                      gtATGAGCGCTTCTGAGCTTAACGAAATTCGCGCTGTAATTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCAcgcc                                             <  2:941983/149‑1 (MQ=255)
                                                                                                       tATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCAcgccg                                            <  1:776740/150‑1 (MQ=255)
                                                                                                       tATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCAcgccg                                            <  1:428253/150‑1 (MQ=255)
                                                                                                           aGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGct                                        >  2:2864781/1‑150 (MQ=255)
                                                                                                           aGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGct                                        <  2:2580582/150‑1 (MQ=255)
                                                                                                            gCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGctc                                       <  1:1223288/150‑1 (MQ=255)
                                                                                                            gCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGctc                                       <  1:1598308/150‑1 (MQ=255)
                                                                                                             cGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGctct                                      >  2:102839/1‑150 (MQ=255)
                                                                                                              gTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTg                                     <  1:2431968/150‑1 (MQ=255)
                                                                                                              gTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTg                                     <  2:2869861/150‑1 (MQ=255)
                                                                                                                 tCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGgcg                                  >  1:551471/1‑150 (MQ=255)
                                                                                                                  cTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGgcgc                                 <  2:3445625/150‑1 (MQ=255)
                                                                                                                          aaCGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAAt                         <  2:3410083/150‑1 (MQ=255)
                                                                                                                          aaCGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAAt                         >  2:153773/1‑150 (MQ=255)
                                                                                                                            cGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATcc                       <  2:3091780/150‑1 (MQ=255)
                                                                                                                             gAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCg                      >  1:2522962/1‑150 (MQ=255)
                                                                                                                              aaaTTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGc                     >  1:3048911/1‑150 (MQ=255)
                                                                                                                               aaTTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCa                    >  1:2053998/1‑150 (MQ=255)
                                                                                                                                  tCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGt                 <  1:4339001/150‑1 (MQ=255)
                                                                                                                                  tCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGt                 <  2:3343217/150‑1 (MQ=255)
                                                                                                                                   cgcgATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTc                <  2:3660110/150‑1 (MQ=255)
                                                                                                                                     cgATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTg              >  2:3807290/1‑150 (MQ=255)
                                                                                                                                        tGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCAGGTCTGGTa           >  2:3625692/1‑150 (MQ=255)
                                                                                                                                         gTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTAt          <  1:3006043/150‑1 (MQ=255)
                                                                                                                                         gTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTAt          <  1:3004866/150‑1 (MQ=255)
                                                                                                                                          tATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATc         <  1:1070480/150‑1 (MQ=255)
                                                                                                                                           aTTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCt        >  1:3161108/1‑150 (MQ=255)
                                                                                                                                             ttGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCttt      <  1:1758856/150‑1 (MQ=255)
                                                                                                                                               gATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTTCg    <  1:1254381/150‑1 (MQ=255)
                                                                                                                                                aTTACCAGTCACATACCCATTTTTTGCATCGGGTCGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACACTATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTTCGt   >  2:4208206/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CGTATCCTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTTCGTA  >  CP009273/142313‑142607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTATCCTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTTCGTATC  >  CP009273/142313‑142609
                                                                                                                                                   |                                                                                                                                                     
CGTATCCTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACC                                                                                                                                                     <  SRR19973080.3789568/150‑1 (MQ=60)
    TCCTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTTATTAACAGTC                                                                                                                                                 >  SRR19973080.3428557/1‑150 (MQ=60)
    TCCTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTC                                                                                                                                                 <  SRR19973080.4411056/150‑1 (MQ=60)
     CCTGTTTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCA                                                                                                                                                >  SRR19973080.1881539/1‑150 (MQ=60)
         TGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATA                                                                                                                                            <  SRR19973080.440527/150‑1 (MQ=60)
            TGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCC                                                                                                                                         <  SRR19973080.4430176/150‑1 (MQ=60)
               AACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATT                                                                                                                                      <  SRR19973080.1290673/150‑1 (MQ=60)
               AACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATT                                                                                                                                      <  SRR19973080.1290670/150‑1 (MQ=60)
                ACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTT                                                                                                                                     >  SRR19973080.4034942/1‑150 (MQ=60)
                      TGGCCTGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCA                                                                                                                               <  SRR19973080.3489970/150‑1 (MQ=60)
                      TGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTAGCGATGTATTTTATTACCAGTCACATACCCATTTTTTGCA                                                                                                                               >  SRR19973080.3069576/1‑150 (MQ=60)
                      TGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACATCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCA                                                                                                                               >  SRR19973080.2435848/1‑150 (MQ=60)
                      TGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACATCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCA                                                                                                                               >  SRR19973080.2435587/1‑150 (MQ=60)
                        GCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATC                                                                                                                             <  SRR19973080.2336721/150‑1 (MQ=60)
                         CATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATTAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTTCATCG                                                                                                                            >  SRR19973080.2365853/1‑150 (MQ=60)
                         CATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCG                                                                                                                            >  SRR19973080.3996528/1‑150 (MQ=60)
                         CATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCG                                                                                                                            <  SRR19973080.349153/150‑1 (MQ=60)
                         CATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCAGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCG                                                                                                                            >  SRR19973080.3996730/1‑150 (MQ=60)
                           TGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGG                                                                                                                          >  SRR19973080.2832970/1‑150 (MQ=60)
                           TGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGG                                                                                                                          >  SRR19973080.2648403/1‑150 (MQ=60)
                            GAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGT                                                                                                                         <  SRR19973080.3063999/150‑1 (MQ=60)
                              AGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCCTTTTTTGCATCGGGTAG                                                                                                                       >  SRR19973080.2016849/1‑150 (MQ=60)
                                  GACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTATGAGCTTAACGAAATTCGCGATGTATTTGATTAACAGTCACATACCCATTTTTTGCATCGGGTAGATGG                                                                                                                   >  SRR19973080.1022645/1‑150 (MQ=60)
                                   ACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGT                                                                                                                  >  SRR19973080.733877/1‑150 (MQ=60)
                                   ACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGT                                                                                                                  >  SRR19973080.3009275/1‑150 (MQ=60)
                                   ACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGT                                                                                                                  >  SRR19973080.733856/1‑150 (MQ=60)
                                   ACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTAACAGTCACATACCCAATTTTTTCATCGGGTAGATGGT                                                                                                                  >  SRR19973080.1583614/1‑150 (MQ=60)
                                     GGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTA                                                                                                                <  SRR19973080.2000670/150‑1 (MQ=60)
                                     GGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTA                                                                                                                <  SRR19973080.2000628/150‑1 (MQ=60)
                                      GCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTAT                                                                                                               <  SRR19973080.1192441/150‑1 (MQ=60)
                                      GCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTAT                                                                                                               <  SRR19973080.3316307/150‑1 (MQ=60)
                                       CGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATC                                                                                                              <  SRR19973080.1104517/150‑1 (MQ=60)
                                       CGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATC                                                                                                              <  SRR19973080.410539/150‑1 (MQ=60)
                                        GTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCG                                                                                                             >  SRR19973080.3019222/1‑150 (MQ=60)
                                        GTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCG                                                                                                             >  SRR19973080.1243650/1‑150 (MQ=60)
                                        GTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCG                                                                                                             >  SRR19973080.3710505/1‑150 (MQ=60)
                                          TTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCC                                                                                                           >  SRR19973080.168961/1‑150 (MQ=60)
                                          TTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCC                                                                                                           >  SRR19973080.168940/1‑150 (MQ=60)
                                           TATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCG                                                                                                          <  SRR19973080.2421522/150‑1 (MQ=60)
                                           TATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCG                                                                                                          <  SRR19973080.1180795/150‑1 (MQ=60)
                                            ATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGA                                                                                                         >  SRR19973080.2501742/1‑150 (MQ=60)
                                             TTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGAC                                                                                                        <  SRR19973080.541467/150‑1 (MQ=60)
                                             TTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGAC                                                                                                        <  SRR19973080.2816360/150‑1 (MQ=60)
                                             TTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGAC                                                                                                        <  SRR19973080.4046086/150‑1 (MQ=60)
                                                 TACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCAT                                                                                                    <  SRR19973080.670538/150‑1 (MQ=60)
                                                 TACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCAT                                                                                                    <  SRR19973080.2605830/150‑1 (MQ=60)
                                                     TCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTA                                                                                                <  SRR19973080.492162/150‑1 (MQ=60)
                                                     TCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTA                                                                                                <  SRR19973080.106907/150‑1 (MQ=60)
                                                       ACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACT                                                                                              <  SRR19973080.4167980/150‑1 (MQ=60)
                                                        CGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTG                                                                                             >  SRR19973080.938907/1‑150 (MQ=60)
                                                           ATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGC                                                                                          >  SRR19973080.3455528/1‑150 (MQ=60)
                                                                ACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAG                                                                                     <  SRR19973080.1619018/150‑1 (MQ=60)
                                                                 CGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGT                                                                                    >  SRR19973080.1447465/1‑150 (MQ=60)
                                                                   TCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGA                                                                                  >  SRR19973080.2198006/1‑150 (MQ=60)
                                                                     ACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGC                                                                                >  SRR19973080.3633448/1‑150 (MQ=60)
                                                                     ACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGC                                                                                >  SRR19973080.3635119/1‑150 (MQ=60)
                                                                      CCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCA                                                                               >  SRR19973080.539816/1‑150 (MQ=60)
                                                                      CCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCA                                                                               <  SRR19973080.1598859/150‑1 (MQ=60)
                                                                      CCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCA                                                                               <  SRR19973080.1599326/150‑1 (MQ=60)
                                                                      CCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCA                                                                               <  SRR19973080.1316851/150‑1 (MQ=60)
                                                                        CGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACA                                                                             <  SRR19973080.904723/150‑1 (MQ=60)
                                                                          CAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAAT                                                                           <  SRR19973080.4021516/150‑1 (MQ=60)
                                                                           AGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATA                                                                          >  SRR19973080.3680293/1‑150 (MQ=60)
                                                                           AGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATA                                                                          >  SRR19973080.3679450/1‑150 (MQ=60)
                                                                           AGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATA                                                                          >  SRR19973080.2023082/1‑150 (MQ=60)
                                                                              AGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTC                                                                       >  SRR19973080.2820614/1‑150 (MQ=60)
                                                                              AGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTC                                                                       <  SRR19973080.4077162/150‑1 (MQ=60)
                                                                                TGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTG                                                                     <  SRR19973080.210820/150‑1 (MQ=60)
                                                                                 GGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGT                                                                    >  SRR19973080.3620795/1‑150 (MQ=60)
                                                                                  GAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTT                                                                   <  SRR19973080.2874558/150‑1 (MQ=60)
                                                                                  GAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTT                                                                   >  SRR19973080.3620134/1‑150 (MQ=60)
                                                                                   AACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTT                                                                  <  SRR19973080.3582104/150‑1 (MQ=60)
                                                                                    ACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTG                                                                 >  SRR19973080.2435570/1‑150 (MQ=60)
                                                                                    ACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTG                                                                 <  SRR19973080.3271220/150‑1 (MQ=60)
                                                                                    ACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTG                                                                 <  SRR19973080.3271387/150‑1 (MQ=60)
                                                                                    ACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTG                                                                 <  SRR19973080.3271430/150‑1 (MQ=60)
                                                                                       CAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATT                                                              <  SRR19973080.2005237/150‑1 (MQ=60)
                                                                                       CAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATT                                                              >  SRR19973080.3015021/1‑150 (MQ=60)
                                                                                         AAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTT                                                            <  SRR19973080.1095948/150‑1 (MQ=60)
                                                                                           ATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGC                                                          >  SRR19973080.2505145/1‑150 (MQ=60)
                                                                                              GCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACG                                                       >  SRR19973080.393441/1‑150 (MQ=60)
                                                                                              GCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACG                                                       >  SRR19973080.393453/1‑150 (MQ=60)
                                                                                               CTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGT                                                      >  SRR19973080.2151392/1‑150 (MQ=60)
                                                                                                  CAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTGCTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCA                                                   >  SRR19973080.3776571/1‑150 (MQ=60)
                                                                                                  CAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCA                                                   >  SRR19973080.2468283/1‑150 (MQ=60)
                                                                                                  CAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCA                                                   >  SRR19973080.1325274/1‑150 (MQ=60)
                                                                                                  CAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCA                                                   >  SRR19973080.3534466/1‑150 (MQ=60)
                                                                                                  CAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCA                                                   >  SRR19973080.1325315/1‑150 (MQ=60)
                                                                                                   AATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCAC                                                  <  SRR19973080.4286846/150‑1 (MQ=60)
                                                                                                   AATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCAC                                                  <  SRR19973080.4286863/150‑1 (MQ=60)
                                                                                                   AATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCAC                                                  <  SRR19973080.2020813/150‑1 (MQ=60)
                                                                                                     TTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGC                                                <  SRR19973080.3127680/150‑1 (MQ=60)
                                                                                                       TATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCG                                              <  SRR19973080.781183/150‑1 (MQ=60)
                                                                                                       TATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCG                                              <  SRR19973080.430896/150‑1 (MQ=60)
                                                                                                        ATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGC                                             >  SRR19973080.3896807/1‑150 (MQ=60)
                                                                                                            GCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTC                                         <  SRR19973080.1607653/150‑1 (MQ=60)
                                                                                                            GCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTC                                         <  SRR19973080.1230688/150‑1 (MQ=60)
                                                                                                              GTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTG                                       <  SRR19973080.2445455/150‑1 (MQ=60)
                                                                                                                 TCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCG                                    >  SRR19973080.554960/1‑150 (MQ=60)
                                                                                                                      GCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCAATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATT                               >  SRR19973080.948915/1‑150 (MQ=60)
                                                                                                                            CGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCC                         >  SRR19973080.3868944/1‑150 (MQ=60)
                                                                                                                             GAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCG                        >  SRR19973080.2536845/1‑150 (MQ=60)
                                                                                                                              AAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGC                       >  SRR19973080.3065420/1‑150 (MQ=60)
                                                                                                                               AATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCA                      >  SRR19973080.4353160/1‑150 (MQ=60)
                                                                                                                               AATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCA                      >  SRR19973080.2065658/1‑150 (MQ=60)
                                                                                                                                  TCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGT                   <  SRR19973080.4362697/150‑1 (MQ=60)
                                                                                                                                     CGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTG                >  SRR19973080.2727507/1‑150 (MQ=60)
                                                                                                                                       ATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGT              >  SRR19973080.2996081/1‑150 (MQ=60)
                                                                                                                                         GTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTAT            <  SRR19973080.3021031/150‑1 (MQ=60)
                                                                                                                                         GTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTAT            <  SRR19973080.3022214/150‑1 (MQ=60)
                                                                                                                                         GTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTAT            <  SRR19973080.3021324/150‑1 (MQ=60)
                                                                                                                                         GTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTAT            >  SRR19973080.170695/1‑150 (MQ=60)
                                                                                                                                          TATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATC           <  SRR19973080.957192/150‑1 (MQ=60)
                                                                                                                                          TATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATC           <  SRR19973080.1076716/150‑1 (MQ=60)
                                                                                                                                          TATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATC           >  SRR19973080.3239574/1‑150 (MQ=60)
                                                                                                                                           ATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCT          >  SRR19973080.3178539/1‑150 (MQ=60)
                                                                                                                                           ATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCT          <  SRR19973080.1118447/150‑1 (MQ=60)
                                                                                                                                           ATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCT          <  SRR19973080.1194999/150‑1 (MQ=60)
                                                                                                                                             TTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTT        <  SRR19973080.1769088/150‑1 (MQ=60)
                                                                                                                                             TTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTT        <  SRR19973080.1768985/150‑1 (MQ=60)
                                                                                                                                              TGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTTC       <  SRR19973080.730505/150‑1 (MQ=60)
                                                                                                                                              TGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTTC       <  SRR19973080.3580839/150‑1 (MQ=60)
                                                                                                                                               GATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTTCG      <  SRR19973080.1261981/150‑1 (MQ=60)
                                                                                                                                                 TTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTTCGTA    >  SRR19973080.2457925/1‑150 (MQ=60)
                                                                                                                                                 TTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTTCGTA    <  SRR19973080.490338/150‑1 (MQ=60)
                                                                                                                                                  TACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTTCGTAT   >  SRR19973080.4134952/1‑150 (MQ=60)
                                                                                                                                                  TACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTTCGTAT   >  SRR19973080.2721830/1‑150 (MQ=60)
                                                                                                                                                  TACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTTCGTAT   >  SRR19973080.4077461/1‑150 (MQ=60)
                                                                                                                                                   ACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTTCGTATC  <  SRR19973080.1466983/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
CGTATCCTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTTCGTATC  >  CP009273/142313‑142609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑