Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 142,460 T→A 65.1% F298Y (TTC→TAC)  yadE → putative polysaccharide deacetylase lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273142,4600TA65.1% 219.2 / 215.7 181F298Y (TTC→TAC) yadEputative polysaccharide deacetylase lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (24/39);  new base A (72/46);  total (96/85)
Fisher's exact test for biased strand distribution p-value = 4.72e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01

Only 100 of 187 total aligned reads displayed.

TGCGTATCCTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTTCGTATC  >  CP009273/142311‑142609
                                                                                                                                                     |                                                                                                                                                     
tGCGTATCCTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGAttt                                                                                                                                                       <  1:1009858/150‑1 (MQ=255)
tGCGTATCCTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGAttt                                                                                                                                                       >  1:4465120/1‑150 (MQ=255)
tGCGTATCCTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGAttt                                                                                                                                                       <  1:1728149/150‑1 (MQ=255)
  cGTATCCTGTGTAGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTAcc                                                                                                                                                     >  2:2775194/1‑150 (MQ=255)
          gtgtTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGTACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACAt                                                                                                                                             <  2:2837883/150‑1 (MQ=255)
          gtgtTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACAt                                                                                                                                             >  1:2326733/1‑150 (MQ=255)
                 caCAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCAtt                                                                                                                                      <  2:4769427/149‑1 (MQ=255)
                   cAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCAtttt                                                                                                                                    <  1:4687720/150‑1 (MQ=255)
                   cAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCAtttt                                                                                                                                    <  1:4011801/150‑1 (MQ=255)
                   cAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCAtttt                                                                                                                                    <  2:3545112/150‑1 (MQ=255)
                    aaTATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCAttttt                                                                                                                                   <  1:1905480/150‑1 (MQ=255)
                    aaTATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCAttttt                                                                                                                                   >  2:536975/1‑150 (MQ=255)
                    aaTATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCAttttt                                                                                                                                   >  2:2327214/1‑150 (MQ=255)
                     atatGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCAtttttt                                                                                                                                  >  2:2524920/1‑150 (MQ=255)
                      tatGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTg                                                                                                                                 >  1:2664745/1‑150 (MQ=255)
                         ggCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCAt                                                                                                                              <  2:734906/150‑1 (MQ=255)
                         ggCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCAt                                                                                                                              <  2:188506/150‑1 (MQ=255)
                          gCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATAGCTTCAATTTATGATCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACAAACCCATTTTTTGCAta                                                                                                                             >  1:3657370/1‑149 (MQ=255)
                            aTGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCgg                                                                                                                           >  2:4021362/1‑150 (MQ=255)
                            aTGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCgg                                                                                                                           <  2:1028943/150‑1 (MQ=255)
                              gAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGCTGTATTTTATTAACAGTCACAAACCCATATTTTTCATCGGGt                                                                                                                         >  1:2839157/1‑150 (MQ=255)
                                aGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAg                                                                                                                       <  1:1293865/150‑1 (MQ=255)
                                   cGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATg                                                                                                                    <  2:1235355/150‑1 (MQ=255)
                                    gACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATgg                                                                                                                   >  2:2357108/1‑150 (MQ=255)
                                      cGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGtt                                                                                                                 <  2:2389712/150‑1 (MQ=255)
                                      cGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGtt                                                                                                                 <  1:3784974/150‑1 (MQ=255)
                                       ggCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTa                                                                                                                <  2:4618259/150‑1 (MQ=255)
                                         cGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATc                                                                                                              <  2:923450/150‑1 (MQ=255)
                                              aTTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGa                                                                                                         >  2:1072171/1‑150 (MQ=255)
                                               ttGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGAc                                                                                                        <  2:4414197/150‑1 (MQ=255)
                                               ttGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGAc                                                                                                        >  1:4379657/1‑150 (MQ=255)
                                                   tACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCat                                                                                                    <  2:4528768/150‑1 (MQ=255)
                                                   tACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCat                                                                                                    <  2:211650/150‑1 (MQ=255)
                                                   tACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCat                                                                                                    <  1:1075323/150‑1 (MQ=255)
                                                      cTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATAtt                                                                                                 <  1:3458868/150‑1 (MQ=255)
                                                        cACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTAc                                                                                               <  1:3426149/150‑1 (MQ=255)
                                                         aCGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACt                                                                                              >  1:1804632/1‑150 (MQ=255)
                                                             aTCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGc                                                                                          >  1:2923860/1‑150 (MQ=255)
                                                              tCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGcc                                                                                         <  1:4623522/150‑1 (MQ=255)
                                                              tCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGcc                                                                                         <  2:636931/150‑1 (MQ=255)
                                                                 aaCGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTa                                                                                      >  1:4024377/1‑150 (MQ=255)
                                                                 aaCGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTa                                                                                      >  1:1597138/1‑150 (MQ=255)
                                                                  aCGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAg                                                                                     <  2:2441982/150‑1 (MQ=255)
                                                                      cACCCGCAGAATTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACTAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTGATAGCCGACCCATATTACTGAGCCGTAGTGAg                                                                                 >  2:3209061/1‑150 (MQ=255)
                                                                      cACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAg                                                                                 >  2:780065/1‑150 (MQ=255)
                                                                          cGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGcaca                                                                             <  1:3601134/150‑1 (MQ=255)
                                                                          cGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGcaca                                                                             <  1:2372022/150‑1 (MQ=255)
                                                                            cAGAAGTGGAACCCTAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACACATATTACTGAGCCGTAGTGAGCACAat                                                                           <  1:767885/150‑1 (MQ=255)
                                                                            cAGAAGTGGAACCCATAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAat                                                                           <  2:3035071/150‑1 (MQ=255)
                                                                              gAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAatat                                                                         <  2:1132075/150‑1 (MQ=255)
                                                                              gAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAatat                                                                         <  2:1132215/150‑1 (MQ=255)
                                                                                aGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTc                                                                       >  1:3213249/1‑150 (MQ=255)
                                                                                aGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTc                                                                       >  1:33276/1‑150 (MQ=255)
                                                                                aGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTc                                                                       >  1:2462604/1‑150 (MQ=255)
                                                                                  tGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTg                                                                     >  2:1078557/1‑150 (MQ=255)
                                                                                  tGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTg                                                                     >  1:3380616/1‑150 (MQ=255)
                                                                                    gAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGtt                                                                   >  1:19908/1‑150 (MQ=255)
                                                                                         cAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGAtt                                                              <  2:2944680/150‑1 (MQ=255)
                                                                                         cAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGAtt                                                              >  2:2395476/1‑150 (MQ=255)
                                                                                           aaaTCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGAtttt                                                            <  2:2489578/150‑1 (MQ=255)
                                                                                            aaTCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTg                                                           <  1:4242546/150‑1 (MQ=255)
                                                                                                gCTGCAATTTATGAGCGTTTATGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACg                                                       >  2:4715023/1‑150 (MQ=255)
                                                                                                 cTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGt                                                      >  2:1896796/1‑150 (MQ=255)
                                                                                                  tGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGtt                                                     >  2:2714126/1‑150 (MQ=255)
                                                                                                  tGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGtt                                                     >  2:282753/1‑150 (MQ=255)
                                                                                                   gCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTc                                                    <  2:4102478/150‑1 (MQ=255)
                                                                                                   gCAATTTATGAGCGTTTCTGAGCTTAACGAAAGTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTc                                                    <  2:4102507/150‑1 (MQ=255)
                                                                                                    cAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCa                                                   >  1:2718146/1‑150 (MQ=255)
                                                                                                    cAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCa                                                   >  1:2718235/1‑150 (MQ=255)
                                                                                                    cAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCa                                                   >  2:547625/1‑150 (MQ=255)
                                                                                                    cAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCa                                                   >  2:784302/1‑150 (MQ=255)
                                                                                                     aaTTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCAc                                                  <  1:1474524/150‑1 (MQ=255)
                                                                                                          aTGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCAcgccgc                                             >  1:4584723/1‑150 (MQ=255)
                                                                                                          aTGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCAcgccgc                                             >  1:3923189/1‑150 (MQ=255)
                                                                                                           tGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCcgcg                                            <  2:2979186/150‑1 (MQ=255)
                                                                                                           tGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCcgcg                                            <  2:1551809/150‑1 (MQ=255)
                                                                                                             aGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGct                                          <  2:163677/150‑1 (MQ=255)
                                                                                                                     tGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCa                                  >  1:1876252/1‑150 (MQ=255)
                                                                                                                      gAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGTTTAACGCCGACCCATATTACTGTGCCGAAGTGAGCACAATATAATGTTTGATTTTGCACTTTCACGCCGCGCTATGGCGCaa                                 >  2:3415416/1‑150 (MQ=255)
                                                                                                                        gCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAAtt                               >  2:2218649/1‑150 (MQ=255)
                                                                                                                         catAACGAAATTCGCGATTTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAAttt                              <  1:2410019/148‑1 (MQ=255)
                                                                                                                          ttAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTa                             <  2:2343940/150‑1 (MQ=255)
                                                                                                                          ttAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTa                             <  2:4555846/150‑1 (MQ=255)
                                                                                                                           tAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTaa                            >  2:2251287/1‑150 (MQ=255)
                                                                                                                              cGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATcc                         >  2:3969672/1‑150 (MQ=255)
                                                                                                                               gAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCg                        >  2:74492/1‑150 (MQ=255)
                                                                                                                                aaaTTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGc                       >  2:2890499/1‑150 (MQ=255)
                                                                                                                                aaaTTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGc                       >  2:3392631/1‑150 (MQ=255)
                                                                                                                                 aaTTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCACTTTAATCCGCa                      >  2:1775253/1‑150 (MQ=255)
                                                                                                                                      gcgATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCt                 <  2:3958753/150‑1 (MQ=255)
                                                                                                                                      gcgATGTATTGGATTTCCAGTCACATACCCATTTTTTGCATCGTGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCt                 <  2:3958700/150‑1 (MQ=255)
                                                                                                                                       cgATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTg                >  2:2389435/1‑150 (MQ=255)
                                                                                                                                        gATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTgg               <  2:1572286/150‑1 (MQ=255)
                                                                                                                                        gATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTgg               <  1:536975/150‑1 (MQ=255)
                                                                                                                                        gATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTgg               <  1:536458/150‑1 (MQ=255)
                                                                                                                                             aTTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCt          <  1:2722827/150‑1 (MQ=255)
                                                                                                                                                  aTTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTCTCTTTCGt     >  1:958252/1‑150 (MQ=255)
                                                                                                                                                   tttCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTTCGTa    <  2:3679331/150‑1 (MQ=255)
                                                                                                                                                     tCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTTCGTATc  >  1:977160/1‑150 (MQ=255)
                                                                                                                                                     tCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTTCGTATc  >  1:3422866/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGCGTATCCTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTTCGTATC  >  CP009273/142311‑142609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCGTATCCTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTTCGTATC  >  CP009273/142311‑142609
                                                                                                                                                     |                                                                                                                                                     
TGCGTATCCTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTT                                                                                                                                                       <  SRR19973081.1016704/150‑1 (MQ=60)
TGCGTATCCTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTT                                                                                                                                                       <  SRR19973081.1739487/150‑1 (MQ=60)
TGCGTATCCTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTT                                                                                                                                                       >  SRR19973081.4491897/1‑150 (MQ=60)
      TCCTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTC                                                                                                                                                 >  SRR19973081.4531098/1‑150 (MQ=60)
          GTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACAT                                                                                                                                             >  SRR19973081.2341278/1‑150 (MQ=60)
              TGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCC                                                                                                                                         <  SRR19973081.4820284/150‑1 (MQ=60)
               GAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTAACAGTCACATACACA                                                                                                                                        >  SRR19973081.4087960/1‑150 (MQ=60)
                   CAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTT                                                                                                                                    <  SRR19973081.4036154/150‑1 (MQ=60)
                   CAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTT                                                                                                                                    <  SRR19973081.4716143/150‑1 (MQ=60)
                    AATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTT                                                                                                                                   <  SRR19973081.1917675/150‑1 (MQ=60)
                      TATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTG                                                                                                                                 >  SRR19973081.2681285/1‑150 (MQ=60)
                          GCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATAGCTTCAATTTATGATCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACAAACCCATTTTTTGCATA                                                                                                                             >  SRR19973081.3679431/1‑150 (MQ=60)
                           CATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCG                                                                                                                            >  SRR19973081.3366727/1‑150 (MQ=60)
                              GAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGCTGTATTTTATTAACAGTCACAAACCCATATTTTTCATCGGGT                                                                                                                         >  SRR19973081.2856337/1‑150 (MQ=60)
                                AGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAG                                                                                                                       <  SRR19973081.1302467/150‑1 (MQ=60)
                                      CGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTT                                                                                                                 <  SRR19973081.3808224/150‑1 (MQ=60)
                                       GGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGTTTACCAGTCACATACCCATTTTTTTCATCGGGTAGTTGGTTA                                                                                                                >  SRR19973081.2476193/1‑150 (MQ=60)
                                          GTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCG                                                                                                             >  SRR19973081.4471023/1‑150 (MQ=60)
                                               TTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGAC                                                                                                        >  SRR19973081.4406128/1‑150 (MQ=60)
                                                   TACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCAT                                                                                                    <  SRR19973081.1082467/150‑1 (MQ=60)
                                                   TACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCAT                                                                                                    <  SRR19973081.1082807/150‑1 (MQ=60)
                                                      CTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATT                                                                                                 <  SRR19973081.3480068/150‑1 (MQ=60)
                                                      CTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATT                                                                                                 <  SRR19973081.3480131/150‑1 (MQ=60)
                                                        CACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTAC                                                                                               <  SRR19973081.3447228/150‑1 (MQ=60)
                                                         ACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACT                                                                                              <  SRR19973081.3829302/150‑1 (MQ=60)
                                                         ACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACT                                                                                              >  SRR19973081.1816410/1‑150 (MQ=60)
                                                             ATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGC                                                                                          >  SRR19973081.2941887/1‑150 (MQ=60)
                                                              TCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCC                                                                                         <  SRR19973081.4651595/150‑1 (MQ=60)
                                                              TCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCC                                                                                         <  SRR19973081.2861972/150‑1 (MQ=60)
                                                                 AACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTA                                                                                      >  SRR19973081.1607374/1‑150 (MQ=60)
                                                                 AACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTA                                                                                      >  SRR19973081.4048772/1‑150 (MQ=60)
                                                                         CCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCAC                                                                              <  SRR19973081.586700/150‑1 (MQ=60)
                                                                          CGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACA                                                                             <  SRR19973081.2386728/150‑1 (MQ=60)
                                                                          CGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACA                                                                             <  SRR19973081.3622967/150‑1 (MQ=60)
                                                                          CGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACA                                                                             <  SRR19973081.2386731/150‑1 (MQ=60)
                                                                            CAGAAGTGGAACCCTAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACACATATTACTGAGCCGTAGTGAGCACAAT                                                                           <  SRR19973081.772829/150‑1 (MQ=60)
                                                                               AAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATT                                                                        >  SRR19973081.3725351/1‑150 (MQ=60)
                                                                                AGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTC                                                                       >  SRR19973081.2478054/1‑150 (MQ=60)
                                                                                AGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTC                                                                       >  SRR19973081.2477866/1‑150 (MQ=60)
                                                                                AGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTC                                                                       >  SRR19973081.3232663/1‑150 (MQ=60)
                                                                                AGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTC                                                                       >  SRR19973081.33686/1‑150 (MQ=60)
                                                                                  TGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTG                                                                     >  SRR19973081.3401402/1‑150 (MQ=60)
                                                                                  TGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTG                                                                     >  SRR19973081.3401653/1‑150 (MQ=60)
                                                                                    GAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTT                                                                   >  SRR19973081.20167/1‑150 (MQ=60)
                                                                                            AATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTG                                                           >  SRR19973081.13426/1‑150 (MQ=60)
                                                                                            AATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTG                                                           <  SRR19973081.4268460/150‑1 (MQ=60)
                                                                                                    CAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCA                                                   >  SRR19973081.2734932/1‑150 (MQ=60)
                                                                                                    CAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCA                                                   >  SRR19973081.2735023/1‑150 (MQ=60)
                                                                                                    CAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCA                                                   >  SRR19973081.2735096/1‑150 (MQ=60)
                                                                                                    CAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCA                                                   <  SRR19973081.2341761/150‑1 (MQ=60)
                                                                                                     AATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCAC                                                  <  SRR19973081.1484299/150‑1 (MQ=60)
                                                                                                     AATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCAC                                                  >  SRR19973081.1718148/1‑150 (MQ=60)
                                                                                                        TTATGAGCGTTTCTGAGCTTAACGAGATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCC                                               >  SRR19973081.2264778/1‑150 (MQ=60)
                                                                                                          ATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGC                                             >  SRR19973081.4612539/1‑150 (MQ=60)
                                                                                                          ATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGC                                             >  SRR19973081.3280818/1‑150 (MQ=60)
                                                                                                          ATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGC                                             >  SRR19973081.3947256/1‑150 (MQ=60)
                                                                                                               CGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCT                                        >  SRR19973081.1944869/1‑150 (MQ=60)
                                                                                                                    CTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGC                                   >  SRR19973081.4699890/1‑150 (MQ=60)
                                                                                                                     TGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCA                                  >  SRR19973081.4381955/1‑150 (MQ=60)
                                                                                                                     TGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCA                                  >  SRR19973081.1888335/1‑150 (MQ=60)
                                                                                                                     TGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCA                                  >  SRR19973081.1888341/1‑150 (MQ=60)
                                                                                                                        GCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATT                               <  SRR19973081.4000913/150‑1 (MQ=60)
                                                                                                                         CTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTT                              >  SRR19973081.4623999/1‑150 (MQ=60)
                                                                                                                         CATAACGAAATTCGCGATTTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTT                              <  SRR19973081.2424837/150‑1 (MQ=60)
                                                                                                                          TTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTA                             <  SRR19973081.3627480/150‑1 (MQ=60)
                                                                                                                              CGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCC                         >  SRR19973081.1746193/1‑150 (MQ=60)
                                                                                                                               GAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCG                        <  SRR19973081.3565591/150‑1 (MQ=60)
                                                                                                                               GAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCG                        >  SRR19973081.851836/1‑150 (MQ=60)
                                                                                                                                       CGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATAAATGAGCCGTCGTGAGCACAATATTCTGTATGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGactc                >  SRR19973081.270445/1‑146 (MQ=60)
                                                                                                                                        GATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGG               <  SRR19973081.540279/150‑1 (MQ=60)
                                                                                                                                        GATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGG               <  SRR19973081.540801/150‑1 (MQ=60)
                                                                                                                                           GTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTAT            >  SRR19973081.1498393/1‑150 (MQ=60)
                                                                                                                                             ATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCT          <  SRR19973081.2739644/150‑1 (MQ=60)
                                                                                                                                              TTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCGT         >  SRR19973081.3133792/1‑150 (MQ=60)
                                                                                                                                                  ATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTCTCTTTCGT     >  SRR19973081.964759/1‑150 (MQ=60)
                                                                                                                                                  ATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTTCGT     >  SRR19973081.279578/1‑150 (MQ=60)
                                                                                                                                                  ATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTTCGT     >  SRR19973081.965484/1‑150 (MQ=60)
                                                                                                                                                     TCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTTCGTATC  >  SRR19973081.3443920/1‑150 (MQ=60)
                                                                                                                                                     TCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTTCGTATC  >  SRR19973081.983810/1‑150 (MQ=60)
                                                                                                                                                     ACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTTCGTATC  <  SRR19973081.1085718/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
TGCGTATCCTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTTCGTATC  >  CP009273/142311‑142609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑