Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 142,460 T→A 100% F298Y (TTC→TAC)  yadE → putative polysaccharide deacetylase lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273142,4600TA99.4% 585.4 / ‑4.5 176F298Y (TTC→TAC) yadEputative polysaccharide deacetylase lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base A (104/71);  total (104/72)
Fisher's exact test for biased strand distribution p-value = 4.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 176 total aligned reads displayed.

CCTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTT  >  CP009273/142318‑142603
                                                                                                                                              |                                                                                                                                               
ccTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGt                                                                                                                                            >  2:2825607/1‑148 (MQ=255)
  tgtgTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTca                                                                                                                                          <  1:2987653/148‑1 (MQ=255)
    tgtTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTcaca                                                                                                                                        <  2:1248337/148‑1 (MQ=255)
      ttGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATa                                                                                                                                      >  1:248843/1‑148 (MQ=255)
            cAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCAtt                                                                                                                                >  1:1005909/1‑148 (MQ=255)
            cAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCAtt                                                                                                                                >  2:1864077/1‑148 (MQ=255)
            cAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCAtt                                                                                                                                <  1:3062648/148‑1 (MQ=255)
              atatGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCAtttt                                                                                                                              >  1:70784/1‑148 (MQ=255)
               tatGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCCAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATAACCAttttt                                                                                                                             >  1:241776/1‑148 (MQ=255)
               tatGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCAttttt                                                                                                                             >  1:241818/1‑148 (MQ=255)
                  ggCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGc                                                                                                                          <  2:406631/148‑1 (MQ=255)
                    cATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCAt                                                                                                                        <  2:833307/148‑1 (MQ=255)
                      tGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCg                                                                                                                      <  1:652328/148‑1 (MQ=255)
                      tGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCg                                                                                                                      >  1:2438296/1‑148 (MQ=255)
                       gAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCgg                                                                                                                     >  2:1968663/1‑148 (MQ=255)
                       gAAGGCGACGGCGTTTAATGTTAACTCACGCAACAAACGTCACCCCCAGAAGTGGAACCCAAAATCGCTGCAATTTATTAGCGTTTATTAGCTTAAAGAAAATCCCGATGTATATGATTACCAGTCACATACACATATTTTGCAACgg                                                                                                                     >  1:2607751/1‑148 (MQ=255)
                        aaGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCggg                                                                                                                    <  2:1867648/148‑1 (MQ=255)
                           gCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAg                                                                                                                 <  2:13866/148‑1 (MQ=255)
                            cGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGa                                                                                                                <  1:1404295/148‑1 (MQ=255)
                              aCGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATg                                                                                                              >  2:3292286/1‑148 (MQ=255)
                                  cGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTa                                                                                                          >  2:1356432/1‑148 (MQ=255)
                                    tttATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATc                                                                                                        >  2:2233747/1‑148 (MQ=255)
                                     ttATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCg                                                                                                       >  1:2286672/1‑148 (MQ=255)
                                     ttATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCg                                                                                                       >  1:134697/1‑148 (MQ=255)
                                         tGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGa                                                                                                   <  1:468038/148‑1 (MQ=255)
                                          gTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGAc                                                                                                  >  1:2715525/1‑148 (MQ=255)
                                          gTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGAc                                                                                                  >  1:2715573/1‑148 (MQ=255)
                                           ttACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGAcc                                                                                                 <  2:2438296/148‑1 (MQ=255)
                                           ttACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGAcc                                                                                                 <  2:2438294/148‑1 (MQ=255)
                                                     cATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTg                                                                                       >  2:1252473/1‑148 (MQ=255)
                                                     cATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTg                                                                                       >  2:1573116/1‑148 (MQ=255)
                                                     cATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTg                                                                                       >  2:1573082/1‑148 (MQ=255)
                                                       tCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAg                                                                                     >  2:1539766/1‑148 (MQ=255)
                                                       tCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAg                                                                                     >  2:779372/1‑148 (MQ=255)
                                                       tCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAg                                                                                     <  1:93813/148‑1 (MQ=255)
                                                           aCGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGt                                                                                 <  1:471910/148‑1 (MQ=255)
                                                            cGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTa                                                                                <  1:2516491/148‑1 (MQ=255)
                                                            cGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTa                                                                                >  1:1760788/1‑148 (MQ=255)
                                                            cGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTa                                                                                <  1:2516346/148‑1 (MQ=255)
                                                            cGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTa                                                                                <  1:3251736/148‑1 (MQ=255)
                                                            cGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTa                                                                                <  1:530105/148‑1 (MQ=255)
                                                              tCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGt                                                                              <  1:1699921/148‑1 (MQ=255)
                                                              tCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGt                                                                              <  1:43850/148‑1 (MQ=255)
                                                                 cccGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAg                                                                           >  2:2551496/1‑148 (MQ=255)
                                                                    gCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGcac                                                                        >  2:1038250/1‑148 (MQ=255)
                                                                      aGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACaa                                                                      <  1:1604557/148‑1 (MQ=255)
                                                                        aaGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAata                                                                    >  1:3040313/1‑148 (MQ=255)
                                                                        aaGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAata                                                                    >  1:2541891/1‑148 (MQ=255)
                                                                        aaGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAata                                                                    >  1:887154/1‑148 (MQ=255)
                                                                         aGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAatat                                                                   <  2:2998896/148‑1 (MQ=255)
                                                                            ggAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCt                                                                >  1:661894/1‑148 (MQ=255)
                                                                             gAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTg                                                               >  1:2115132/1‑148 (MQ=255)
                                                                             gAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTg                                                               >  2:570206/1‑148 (MQ=255)
                                                                              aaCCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGt                                                              >  1:2264037/1‑148 (MQ=255)
                                                                                cccAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGACATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGttt                                                            >  2:802895/1‑148 (MQ=255)
                                                                                cccAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGttt                                                            >  2:3015592/1‑148 (MQ=255)
                                                                                cccAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGttt                                                            >  2:600514/1‑148 (MQ=255)
                                                                                  cAAAATCGCTGCAATTTATGATCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGa                                                          <  2:925461/148‑1 (MQ=255)
                                                                                   aaaaTCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGAt                                                         >  2:913855/1‑148 (MQ=255)
                                                                                       tCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTg                                                     <  2:2066714/148‑1 (MQ=255)
                                                                                        cGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGc                                                    >  2:1784360/1‑148 (MQ=255)
                                                                                         gCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCa                                                   <  2:2683525/148‑1 (MQ=255)
                                                                                         gCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCa                                                   <  2:3300167/148‑1 (MQ=255)
                                                                                         gCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGAGTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCa                                                   <  2:2683597/148‑1 (MQ=255)
                                                                                           tGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACg                                                 <  2:3077085/148‑1 (MQ=255)
                                                                                           tGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACg                                                 >  2:2903277/1‑148 (MQ=255)
                                                                                           tGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACg                                                 >  2:2903469/1‑148 (MQ=255)
                                                                                            gCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGt                                                <  1:3135458/148‑1 (MQ=255)
                                                                                            gCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGt                                                >  1:2435215/1‑148 (MQ=255)
                                                                                              aaTTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTc                                              <  1:1909340/148‑1 (MQ=255)
                                                                                                tttATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGAACCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCAc                                            >  2:2610152/1‑148 (MQ=255)
                                                                                                  tATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCAcgc                                          <  2:1679322/148‑1 (MQ=255)
                                                                                                     gAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCAcgccgc                                       >  1:2393408/1‑148 (MQ=255)
                                                                                                      aGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCcgcg                                      <  1:488044/148‑1 (MQ=255)
                                                                                                        cttttCTGAGATTAACGAAATTCGCGATGTATTTTATTACCAGTAACATACCCATTTTTTGCATCGGGTTGATGGTTATGGCCGACCCATATTACTGAGCAGTAGTGAGCACAATTTTATGTTTGATTTTGCACGTTCACGCCGCGct                                    <  1:1059522/146‑1 (MQ=255)
                                                                                                        cGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGct                                    >  2:797569/1‑148 (MQ=255)
                                                                                                        cGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGct                                    <  2:679270/148‑1 (MQ=255)
                                                                                                        cGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATGTTGCACGTTCACGCCGCGct                                    >  2:2747861/1‑148 (MQ=255)
                                                                                                           ttCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTg                                 >  1:229139/1‑148 (MQ=255)
                                                                                                           ttCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTg                                 >  1:229467/1‑148 (MQ=255)
                                                                                                           ttCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTg                                 >  1:231273/1‑148 (MQ=255)
                                                                                                                 gCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCaa                           >  2:1969831/1‑148 (MQ=255)
                                                                                                                   ttAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAAtt                         >  1:1144969/1‑148 (MQ=255)
                                                                                                                    tAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAAttt                        >  1:1879729/1‑148 (MQ=255)
                                                                                                                      aCGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTaa                      >  2:2858342/1‑148 (MQ=255)
                                                                                                                       cGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAAt                     <  1:503828/148‑1 (MQ=255)
                                                                                                                            ttCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCa                >  1:278696/1‑148 (MQ=255)
                                                                                                                             tCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCAt               >  1:740246/1‑148 (MQ=255)
                                                                                                                             tCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCAt               >  2:2778386/1‑148 (MQ=255)
                                                                                                                                cgATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTc            <  2:3007739/148‑1 (MQ=255)
                                                                                                                                cgATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTc            <  1:3209913/148‑1 (MQ=255)
                                                                                                                                  aTGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTg          >  1:2756970/1‑148 (MQ=255)
                                                                                                                                  aTGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTg          <  1:1320751/148‑1 (MQ=255)
                                                                                                                                     tATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTa       >  2:2460378/1‑148 (MQ=255)
                                                                                                                                       tttGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATc     <  2:180554/148‑1 (MQ=255)
                                                                                                                                       tttGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATc     >  1:2224243/1‑148 (MQ=255)
                                                                                                                                       tttGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATc     <  1:1335088/148‑1 (MQ=255)
                                                                                                                                        ttGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCt    <  1:80634/148‑1 (MQ=255)
                                                                                                                                          gATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCttt  <  1:882476/148‑1 (MQ=255)
                                                                                                                                          gATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCttt  <  2:70817/148‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                               
CCTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTT  >  CP009273/142318‑142603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCGTATCCTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTTCGT  >  CP009273/142311‑142606
                                                                                                                                                     |                                                                                                                                                  
TGCGTATCCTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTA                                                                                                                                                    <  SRR19973070.748078/150‑1 (MQ=60)
  CGTATCCTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACC                                                                                                                                                  <  SRR19973070.955536/150‑1 (MQ=60)
  CGTATCCTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACC                                                                                                                                                  <  SRR19973070.1705872/150‑1 (MQ=60)
     ATCCTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGT                                                                                                                                               >  SRR19973070.275220/1‑150 (MQ=60)
         TGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACA                                                                                                                                           <  SRR19973070.3006800/150‑1 (MQ=60)
           TGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATA                                                                                                                                         >  SRR19973070.250544/1‑150 (MQ=60)
            GTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATAC                                                                                                                                        <  SRR19973070.835019/150‑1 (MQ=60)
                 AACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATT                                                                                                                                   >  SRR19973070.1013077/1‑150 (MQ=60)
                   CAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTT                                                                                                                                 <  SRR19973070.3082259/150‑1 (MQ=60)
                   CAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTT                                                                                                                                 <  SRR19973070.3081994/150‑1 (MQ=60)
                   CAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTT                                                                                                                                 >  SRR19973070.70986/1‑150 (MQ=60)
                   CAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTT                                                                                                                                 >  SRR19973070.71555/1‑150 (MQ=60)
                   CAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTT                                                                                                                                 >  SRR19973070.71588/1‑150 (MQ=60)
                    AATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCCAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATAACCATTTTT                                                                                                                                >  SRR19973070.243443/1‑150 (MQ=60)
                    AATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTT                                                                                                                                >  SRR19973070.243485/1‑150 (MQ=60)
                           CATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCG                                                                                                                         >  SRR19973070.2454276/1‑150 (MQ=60)
                           CATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCG                                                                                                                         >  SRR19973070.2454278/1‑150 (MQ=60)
                            ATGAAGGCGACGGCGTTTAATGTTAACTCACGCAACAAACGTCACCCCCAGAAGTGGAACCCAAAATCGCTGCAATTTATTAGCGTTTATTAGCTTAAAGAAAATCCCGATGTATATGATTACCAGTCACATACACATATTTTGCAACGG                                                                                                                        >  SRR19973070.2624776/1‑150 (MQ=60)
                             TGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGG                                                                                                                       <  SRR19973070.656980/150‑1 (MQ=60)
                              GAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGT                                                                                                                      >  SRR19973070.2511825/1‑150 (MQ=60)
                                   CGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATG                                                                                                                 <  SRR19973070.1413812/150‑1 (MQ=60)
                                         CGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATC                                                                                                           >  SRR19973070.2783331/1‑150 (MQ=60)
                                         CGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATC                                                                                                           >  SRR19973070.944085/1‑150 (MQ=60)
                                          GTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCG                                                                                                          >  SRR19973070.498541/1‑150 (MQ=60)
                                          GTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCG                                                                                                          >  SRR19973070.2301882/1‑150 (MQ=60)
                                          GTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCG                                                                                                          >  SRR19973070.135868/1‑150 (MQ=60)
                                               TTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGAC                                                                                                     >  SRR19973070.2305209/1‑150 (MQ=60)
                                               TTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGAC                                                                                                     >  SRR19973070.2733063/1‑150 (MQ=60)
                                               TTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGAC                                                                                                     >  SRR19973070.2733111/1‑150 (MQ=60)
                                                TGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACC                                                                                                    <  SRR19973070.471364/150‑1 (MQ=60)
                                                      CTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATT                                                                                              >  SRR19973070.1339112/1‑150 (MQ=60)
                                                              TCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCC                                                                                      <  SRR19973070.94739/150‑1 (MQ=60)
                                                                AAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGT                                                                                    >  SRR19973070.1914808/1‑150 (MQ=60)
                                                                 AACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTA                                                                                   >  SRR19973070.1772975/1‑150 (MQ=60)
                                                                  ACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAG                                                                                  <  SRR19973070.475249/150‑1 (MQ=60)
                                                                  ACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAG                                                                                  <  SRR19973070.475236/150‑1 (MQ=60)
                                                                   CGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGT                                                                                 <  SRR19973070.2532738/150‑1 (MQ=60)
                                                                   CGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGT                                                                                 <  SRR19973070.2532884/150‑1 (MQ=60)
                                                                   CGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGT                                                                                 <  SRR19973070.3272689/150‑1 (MQ=60)
                                                                   CGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGT                                                                                 <  SRR19973070.533670/150‑1 (MQ=60)
                                                                   CGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGT                                                                                 <  SRR19973070.533685/150‑1 (MQ=60)
                                                                    GTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTG                                                                                >  SRR19973070.2486866/1‑150 (MQ=60)
                                                                     TCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGA                                                                               <  SRR19973070.410209/150‑1 (MQ=60)
                                                                     TCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGA                                                                               <  SRR19973070.44407/150‑1 (MQ=60)
                                                                     TCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGA                                                                               <  SRR19973070.1711632/150‑1 (MQ=60)
                                                                             AGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATA                                                                       >  SRR19973070.893418/1‑150 (MQ=60)
                                                                             AGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATA                                                                       <  SRR19973070.1615751/150‑1 (MQ=60)
                                                                             AGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATA                                                                       >  SRR19973070.3059643/1‑150 (MQ=60)
                                                                             AGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATA                                                                       >  SRR19973070.2558512/1‑150 (MQ=60)
                                                                                 GTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCT                                                                   >  SRR19973070.666615/1‑150 (MQ=60)
                                                                                  TGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTG                                                                  >  SRR19973070.2129312/1‑150 (MQ=60)
                                                                                   GGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGT                                                                 >  SRR19973070.2279060/1‑150 (MQ=60)
                                                                                   GGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGT                                                                 >  SRR19973070.2279142/1‑150 (MQ=60)
                                                                                       CCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGA                                                             <  SRR19973070.265063/150‑1 (MQ=60)
                                                                                             ATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGC                                                       >  SRR19973070.2899734/1‑150 (MQ=60)
                                                                                               CGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTGGTGAGCACAATATTCTGTTTGATTTTGCAC                                                     >  SRR19973070.961627/1‑150 (MQ=60)
                                                                                               CGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCAC                                                     <  SRR19973070.212300/150‑1 (MQ=60)
                                                                                                GCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACG                                                    <  SRR19973070.2248473/150‑1 (MQ=60)
                                                                                                 CTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGT                                                   >  SRR19973070.2451188/1‑150 (MQ=60)
                                                                                                 CTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGT                                                   >  SRR19973070.1263328/1‑150 (MQ=60)
                                                                                                 CTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGT                                                   >  SRR19973070.3189582/1‑150 (MQ=60)
                                                                                                   GCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTC                                                 <  SRR19973070.3155710/150‑1 (MQ=60)
                                                                                                    CAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCA                                                >  SRR19973070.2320517/1‑150 (MQ=60)
                                                                                                    CAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCA                                                >  SRR19973070.2443514/1‑150 (MQ=60)
                                                                                                     AATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCAC                                               <  SRR19973070.1922240/150‑1 (MQ=60)
                                                                                                     AATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCAC                                               <  SRR19973070.619202/150‑1 (MQ=60)
                                                                                                       TTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGC                                             >  SRR19973070.3129785/1‑150 (MQ=60)
                                                                                                        TTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCC                                            >  SRR19973070.2550975/1‑150 (MQ=60)
                                                                                                          ATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGC                                          >  SRR19973070.2409209/1‑150 (MQ=60)
                                                                                                             AGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCT                                       <  SRR19973070.491470/150‑1 (MQ=60)
                                                                                                               CTTTTCTGAGATTAACGAAATTCGCGATGTATTTTATTACCAGTAACATACCCATTTTTTGCATCGGGTTGATGGTTATGGCCGACCCATATTACTGAGCAGTAGTGAGCACAATTTTATGTTTGATTTTGCACGTTCACGCCGCGCTGT                                     <  SRR19973070.1066956/150‑1 (MQ=60)
                                                                                                                GTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTG                                    >  SRR19973070.1809449/1‑150 (MQ=60)
                                                                                                                GTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTG                                    >  SRR19973070.232906/1‑150 (MQ=60)
                                                                                                                GTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTG                                    >  SRR19973070.231093/1‑150 (MQ=60)
                                                                                                                GTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTG                                    >  SRR19973070.230765/1‑150 (MQ=60)
                                                                                                                        GCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATT                            >  SRR19973070.1152907/1‑150 (MQ=60)
                                                                                                                        GCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATT                            >  SRR19973070.1152922/1‑150 (MQ=60)
                                                                                                                         CTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTT                           >  SRR19973070.1505061/1‑150 (MQ=60)
                                                                                                                         CTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTT                           >  SRR19973070.1892517/1‑150 (MQ=60)
                                                                                                                            AACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAAT                        >  SRR19973070.2608061/1‑150 (MQ=60)
                                                                                                                            AACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAAT                        <  SRR19973070.1365700/150‑1 (MQ=60)
                                                                                                                              CGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCC                      <  SRR19973070.507321/150‑1 (MQ=60)
                                                                                                                                 AATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCA                   >  SRR19973070.280499/1‑150 (MQ=60)
                                                                                                                                  ATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCAT                  >  SRR19973070.745481/1‑150 (MQ=60)
                                                                                                                                  ATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCAT                  >  SRR19973070.2415884/1‑150 (MQ=60)
                                                                                                                                       CGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTG             >  SRR19973070.458551/1‑150 (MQ=60)
                                                                                                                                       CGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTG             >  SRR19973070.2774660/1‑150 (MQ=60)
                                                                                                                                       CGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTG             <  SRR19973070.3230676/150‑1 (MQ=60)
                                                                                                                                         ATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGT           <  SRR19973070.1329885/150‑1 (MQ=60)
                                                                                                                                            TATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATC        >  SRR19973070.2238848/1‑150 (MQ=60)
                                                                                                                                              TTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTT      <  SRR19973070.2394926/150‑1 (MQ=60)
                                                                                                                                              TTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTT      <  SRR19973070.1344279/150‑1 (MQ=60)
                                                                                                                                               TTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTT     <  SRR19973070.1683435/150‑1 (MQ=60)
                                                                                                                                               TTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTT     <  SRR19973070.81366/150‑1 (MQ=60)
                                                                                                                                               TTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTT     <  SRR19973070.81472/150‑1 (MQ=60)
                                                                                                                                                 GATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTTCG   <  SRR19973070.888698/150‑1 (MQ=60)
                                                                                                                                                  ATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTTCGT  >  SRR19973070.2377397/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                  
TGCGTATCCTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTTCGT  >  CP009273/142311‑142606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑