Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 456,588 G→T 87.9% D749Y (GAC→TAC)  lon → DNA‑binding ATP‑dependent protease La

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273456,5880GT87.9% 713.5 / 92.5 257D749Y (GAC→TAC) lonDNA‑binding ATP‑dependent protease La
Reads supporting (aligned to +/- strand):  ref base G (22/9);  new base T (106/120);  total (128/129)
Fisher's exact test for biased strand distribution p-value = 1.31e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

Only 100 of 257 total aligned reads displayed.

GATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCT  >  CP009273/456441‑456734
                                                                                                                                                   |                                                                                                                                                  
   gtgGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAAc                                                                                                                                               <  2:1764430/150‑1 (MQ=255)
       cAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTaa                                                                                                                                           <  2:1431783/150‑1 (MQ=255)
        aaTGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAAt                                                                                                                                          <  1:519116/150‑1 (MQ=255)
         aTGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAAtt                                                                                                                                         <  1:1139640/150‑1 (MQ=255)
                gTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATc                                                                                                                                  >  1:906642/1‑150 (MQ=255)
                  gagcTCACTCAGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTg                                                                                                                                <  1:3144383/150‑1 (MQ=255)
                       cACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACAt                                                                                                                           <  2:435881/150‑1 (MQ=255)
                                  gTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGa                                                                                                                >  2:1957491/1‑150 (MQ=255)
                                     aGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAgc                                                                                                             >  1:1906793/1‑150 (MQ=255)
                                      ggTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAgcg                                                                                                            <  1:1558153/150‑1 (MQ=255)
                                      ggTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAgcg                                                                                                            <  2:486301/150‑1 (MQ=255)
                                        tACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCa                                                                                                          >  2:1480459/1‑150 (MQ=255)
                                        tACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCa                                                                                                          <  2:1001294/150‑1 (MQ=255)
                                          cTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCAtt                                                                                                        >  1:39357/1‑150 (MQ=255)
                                              cGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAgg                                                                                                    >  1:1332522/1‑150 (MQ=255)
                                              cGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAgg                                                                                                    >  1:197965/1‑150 (MQ=255)
                                                aTCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGaa                                                                                                  <  1:1185763/150‑1 (MQ=255)
                                                   ggtggtTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCATGTGAAGCGCATTGAGGAAGtt                                                                                               >  2:975280/1‑150 (MQ=255)
                                                     tggtTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCt                                                                                             <  2:3121790/150‑1 (MQ=255)
                                                     tggtTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCt                                                                                             <  2:2685697/150‑1 (MQ=255)
                                                      ggtTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTg                                                                                            <  1:2369317/150‑1 (MQ=255)
                                                       gtTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGa                                                                                           >  1:2259308/1‑150 (MQ=255)
                                                       gtTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGa                                                                                           >  2:2030361/1‑150 (MQ=255)
                                                          agaaagaaaAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGActc                                                                                        <  1:2647442/149‑1 (MQ=255)
                                                           gaaagaaaAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGActct                                                                                       <  2:879094/150‑1 (MQ=255)
                                                           gaaagaaaAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGActct                                                                                       <  1:4257375/150‑1 (MQ=255)
                                                           gaaagaaaAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGActct                                                                                       <  2:3146727/150‑1 (MQ=255)
                                                             aagaaaAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTgg                                                                                     >  2:317626/1‑150 (MQ=255)
                                                             aagaaaAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTgg                                                                                     >  2:3115360/1‑150 (MQ=255)
                                                             aagaaaAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTgg                                                                                     >  2:2582282/1‑150 (MQ=255)
                                                              agaaaAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGgc                                                                                    >  2:264344/1‑150 (MQ=255)
                                                               gaaaAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGgcg                                                                                   <  2:3851621/150‑1 (MQ=255)
                                                               gaaaAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGgcg                                                                                   >  1:4531409/1‑150 (MQ=255)
                                                               gaaaAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGgcg                                                                                   >  1:4531202/1‑150 (MQ=255)
                                                                aaaaaCTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGgcgc                                                                                  >  1:2642498/1‑150 (MQ=255)
                                                                 aaaaCTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCt                                                                                 <  2:1039323/150‑1 (MQ=255)
                                                                  aaaCTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTg                                                                                <  2:2272696/150‑1 (MQ=255)
                                                                    aCTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCa                                                                              <  2:2381613/150‑1 (MQ=255)
                                                                    aCTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCa                                                                              <  2:3794760/150‑1 (MQ=255)
                                                                    aCTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCa                                                                              <  2:3917795/150‑1 (MQ=255)
                                                                    aCTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCa                                                                              >  1:1413508/1‑150 (MQ=255)
                                                                    aCTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCa                                                                              <  2:2214562/150‑1 (MQ=255)
                                                                    aCTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCa                                                                              >  1:2460490/1‑150 (MQ=255)
                                                                      tCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCaaa                                                                            >  1:3539521/1‑150 (MQ=255)
                                                                        cTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAAt                                                                          <  1:1953904/150‑1 (MQ=255)
                                                                        cTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAAt                                                                          <  2:3167623/150‑1 (MQ=255)
                                                                             aGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAAcc                                                                     <  2:2083741/150‑1 (MQ=255)
                                                                             aGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAAcc                                                                     >  2:1499454/1‑150 (MQ=255)
                                                                               cgcATCGCTGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGt                                                                   >  2:3928308/1‑150 (MQ=255)
                                                                                gcATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTc                                                                  <  2:3433760/150‑1 (MQ=255)
                                                                                gcATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTc                                                                  <  2:4293157/150‑1 (MQ=255)
                                                                                 cATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCt                                                                 <  2:3075886/150‑1 (MQ=255)
                                                                                    cgcgGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGt                                                              <  2:3489140/150‑1 (MQ=255)
                                                                                     gcggcgGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTa                                                             <  1:1692227/150‑1 (MQ=255)
                                                                                       ggcggGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATg                                                           <  1:3632220/150‑1 (MQ=255)
                                                                                         cggGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCa                                                         >  1:2958797/1‑150 (MQ=255)
                                                                                          gggATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCTTTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAg                                                        <  1:3672896/150‑1 (MQ=255)
                                                                                             aTTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGtt                                                     <  1:22353/150‑1 (MQ=255)
                                                                                               tAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTtgt                                                   <  1:839842/150‑1 (MQ=255)
                                                                                                  aaCAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGAc                                                >  1:1487604/1‑150 (MQ=255)
                                                                                                   aCAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACt                                               <  1:3791637/150‑1 (MQ=255)
                                                                                                    cAGTTCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTg                                              <  2:3172721/150‑1 (MQ=255)
                                                                                                     aGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGc                                             >  2:1395489/1‑150 (MQ=255)
                                                                                                      gTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCa                                            >  2:854602/1‑150 (MQ=255)
                                                                                                       tGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCaa                                           >  1:1613424/1‑150 (MQ=255)
                                                                                                           aaTTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATa                                       >  1:3339344/1‑150 (MQ=255)
                                                                                                           aaTTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATa                                       >  1:2096670/1‑150 (MQ=255)
                                                                                                           aaTTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATa                                       >  1:3411706/1‑150 (MQ=255)
                                                                                                            agtCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAg                                      <  1:4049077/148‑1 (MQ=255)
                                                                                                              tCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTg                                    <  1:205489/150‑1 (MQ=255)
                                                                                                                cGTTCGAAAATCAACGCGATGTGGAAGAGATTCCTTACACCGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCGGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGAc                                  <  2:3322587/150‑1 (MQ=255)
                                                                                                                 gTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGAcc                                 <  2:4079010/150‑1 (MQ=255)
                                                                                                                   tCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTc                               <  1:3706751/150‑1 (MQ=255)
                                                                                                                     gAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTcgc                             >  1:2910230/1‑150 (MQ=255)
                                                                                                                      aaaaTAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTcgcg                            >  1:2607284/1‑150 (MQ=255)
                                                                                                                        aaTAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCa                          <  2:3701984/150‑1 (MQ=255)
                                                                                                                        aaTAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCa                          <  2:797979/150‑1 (MQ=255)
                                                                                                                         aTAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCaa                         >  1:2821600/1‑150 (MQ=255)
                                                                                                                           aaaCGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCaaaa                       >  2:2445984/1‑150 (MQ=255)
                                                                                                                           aaaCGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCaaaa                       >  2:2154036/1‑150 (MQ=255)
                                                                                                                            aaCGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAAt                      <  1:430295/150‑1 (MQ=255)
                                                                                                                            aaCGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAAt                      >  2:1973717/1‑150 (MQ=255)
                                                                                                                                cgATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCAc                  <  2:1309829/150‑1 (MQ=255)
                                                                                                                                cgATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCAc                  <  2:2124825/150‑1 (MQ=255)
                                                                                                                                 gATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACt                 <  2:67259/150‑1 (MQ=255)
                                                                                                                                  aTCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACta                >  1:3598137/1‑150 (MQ=255)
                                                                                                                                   tCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACtaa               >  2:4193305/1‑150 (MQ=255)
                                                                                                                                    cTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACtaat              <  1:95640/150‑1 (MQ=255)
                                                                                                                                    cTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACtaat              >  1:450656/1‑150 (MQ=255)
                                                                                                                                     tGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACtaata             >  2:1626441/1‑150 (MQ=255)
                                                                                                                                     tGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACtaata             >  1:2808089/1‑150 (MQ=255)
                                                                                                                                      ggAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACtaataa            >  2:4383186/1‑150 (MQ=255)
                                                                                                                                      ggAAGAGATTCCTTACAACGTAAGTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACtaataa            <  2:2427850/150‑1 (MQ=255)
                                                                                                                                        aaGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATaaaa          <  2:152930/150‑1 (MQ=255)
                                                                                                                                        aaGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATaaaa          >  2:498662/1‑150 (MQ=255)
                                                                                                                                         agagATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATaaaaa         >  1:4052415/1‑150 (MQ=255)
                                                                                                                                          gagaTTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAAc        <  1:3191246/150‑1 (MQ=255)
                                                                                                                                            gaTTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAg      >  1:799187/1‑150 (MQ=255)
                                                                                                                                             aTTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAgg     >  1:3007353/1‑150 (MQ=255)
                                                                                                                                                ccTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCt  >  2:4483424/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCT  >  CP009273/456441‑456734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGG  >  CP009273/456439‑456736
                                                                                                                                                     |                                                                                                                                                    
CCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTT                                                                                                                                                      <  SRR19973080.1934749/150‑1 (MQ=60)
  GATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTAC                                                                                                                                                    >  SRR19973080.4265237/1‑150 (MQ=60)
   ATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACA                                                                                                                                                   <  SRR19973080.4322930/150‑1 (MQ=60)
         CAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAA                                                                                                                                             >  SRR19973080.3560094/1‑150 (MQ=60)
          AATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAAT                                                                                                                                            <  SRR19973080.522419/150‑1 (MQ=60)
          AATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAAT                                                                                                                                            <  SRR19973080.522423/150‑1 (MQ=60)
          AATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAAT                                                                                                                                            <  SRR19973080.522502/150‑1 (MQ=60)
          AATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAAT                                                                                                                                            >  SRR19973080.2036701/1‑150 (MQ=60)
           ATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATT                                                                                                                                           <  SRR19973080.1146299/150‑1 (MQ=60)
                CGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGA                                                                                                                                      >  SRR19973080.3310373/1‑150 (MQ=60)
                  GTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATC                                                                                                                                    >  SRR19973080.912188/1‑150 (MQ=60)
                   TGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCT                                                                                                                                   >  SRR19973080.2871387/1‑150 (MQ=60)
                   TGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCT                                                                                                                                   >  SRR19973080.2872180/1‑150 (MQ=60)
                    GAGCTCACTCAGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTG                                                                                                                                  <  SRR19973080.3161698/150‑1 (MQ=60)
                    GAGATCTCTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGACAAACTCCTGGCTGCGCATCGCGGCGGGATTAAATCAGTGCTAAGTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTT                                                                                                                                  <  SRR19973080.3161770/150‑1 (MQ=60)
                     AGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGG                                                                                                                                 <  SRR19973080.783174/150‑1 (MQ=60)
                     AGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGG                                                                                                                                 <  SRR19973080.3948540/150‑1 (MQ=60)
                       ATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGAC                                                                                                                               <  SRR19973080.1365038/150‑1 (MQ=60)
                         CACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACAT                                                                                                                             <  SRR19973080.2649424/150‑1 (MQ=60)
                          ACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATT                                                                                                                            <  SRR19973080.2578823/150‑1 (MQ=60)
                           CTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTC                                                                                                                           >  SRR19973080.2425164/1‑150 (MQ=60)
                           CTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTC                                                                                                                           >  SRR19973080.14574/1‑150 (MQ=60)
                                      CAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAG                                                                                                                <  SRR19973080.4530299/150‑1 (MQ=60)
                                       AGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGC                                                                                                               >  SRR19973080.1917502/1‑150 (MQ=60)
                                        GGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCG                                                                                                              <  SRR19973080.1567201/150‑1 (MQ=60)
                                            CTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATT                                                                                                          <  SRR19973080.2648127/150‑1 (MQ=60)
                                            CTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATT                                                                                                          >  SRR19973080.39769/1‑150 (MQ=60)
                                            CTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATT                                                                                                          >  SRR19973080.2318265/1‑150 (MQ=60)
                                                CGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGG                                                                                                      >  SRR19973080.1340556/1‑150 (MQ=60)
                                                CGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGG                                                                                                      >  SRR19973080.199445/1‑150 (MQ=60)
                                                CGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGG                                                                                                      >  SRR19973080.917806/1‑150 (MQ=60)
                                                  ATCTGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAA                                                                                                    <  SRR19973080.3496313/150‑1 (MQ=60)
                                                  ATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAA                                                                                                    <  SRR19973080.1192864/150‑1 (MQ=60)
                                                  ATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAA                                                                                                    <  SRR19973080.1194124/150‑1 (MQ=60)
                                                  ATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAA                                                                                                    >  SRR19973080.4006050/1‑150 (MQ=60)
                                                    CGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGT                                                                                                  <  SRR19973080.3654400/150‑1 (MQ=60)
                                                        GGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTG                                                                                              <  SRR19973080.996545/150‑1 (MQ=60)
                                                        GGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTG                                                                                              <  SRR19973080.2382497/150‑1 (MQ=60)
                                                         GTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGA                                                                                             >  SRR19973080.3092660/1‑150 (MQ=60)
                                                         GTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGA                                                                                             >  SRR19973080.2271645/1‑150 (MQ=60)
                                                            TGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTC                                                                                          <  SRR19973080.2661070/150‑1 (MQ=60)
                                                            AGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTC                                                                                          <  SRR19973080.2661718/150‑1 (MQ=60)
                                                             GAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCT                                                                                         <  SRR19973080.4280597/150‑1 (MQ=60)
                                                             GAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCT                                                                                         >  SRR19973080.4554077/1‑150 (MQ=60)
                                                               AAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGG                                                                                       >  SRR19973080.2234860/1‑150 (MQ=60)
                                                               AAGAAAAACTCCGGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGG                                                                                       <  SRR19973080.902614/150‑1 (MQ=60)
                                                                 GAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCG                                                                                     >  SRR19973080.4555804/1‑150 (MQ=60)
                                                                 GAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCG                                                                                     >  SRR19973080.4555718/1‑150 (MQ=60)
                                                                 GAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCG                                                                                     >  SRR19973080.4555596/1‑150 (MQ=60)
                                                                  AAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGC                                                                                    >  SRR19973080.2656752/1‑150 (MQ=60)
                                                                   AAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCT                                                                                   <  SRR19973080.1586555/150‑1 (MQ=60)
                                                                    AAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTG                                                                                  <  SRR19973080.149014/150‑1 (MQ=60)
                                                                      ACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCA                                                                                >  SRR19973080.2474114/1‑150 (MQ=60)
                                                                      ACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCA                                                                                >  SRR19973080.1421842/1‑150 (MQ=60)
                                                                        TCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAA                                                                              >  SRR19973080.3559103/1‑150 (MQ=60)
                                                                          CTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAAT                                                                            <  SRR19973080.1974263/150‑1 (MQ=60)
                                                                          CTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAAT                                                                            <  SRR19973080.1964907/150‑1 (MQ=60)
                                                                          CTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAAT                                                                            <  SRR19973080.1964980/150‑1 (MQ=60)
                                                                            GGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGA                                                                          >  SRR19973080.3605151/1‑150 (MQ=60)
                                                                               AGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACC                                                                       >  SRR19973080.1787977/1‑150 (MQ=60)
                                                                                       GCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTA                                                               <  SRR19973080.1702068/150‑1 (MQ=60)
                                                                                       GCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTA                                                               <  SRR19973080.1702082/150‑1 (MQ=60)
                                                                                        CGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTAT                                                              >  SRR19973080.2255905/1‑150 (MQ=60)
                                                                                         GGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATG                                                             <  SRR19973080.3652285/150‑1 (MQ=60)
                                                                                         GGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATG                                                             <  SRR19973080.3652284/150‑1 (MQ=60)
                                                                                           CGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCA                                                           >  SRR19973080.2974843/1‑150 (MQ=60)
                                                                                           CGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCA                                                           >  SRR19973080.2974832/1‑150 (MQ=60)
                                                                                           CGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCA                                                           >  SRR19973080.1861559/1‑150 (MQ=60)
                                                                                            GGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCTTTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAG                                                          <  SRR19973080.3693120/150‑1 (MQ=60)
                                                                                               ATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTT                                                       <  SRR19973080.22606/150‑1 (MQ=60)
                                                                                                 TAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGT                                                     <  SRR19973080.846095/150‑1 (MQ=60)
                                                                                                 TAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGT                                                     <  SRR19973080.844900/150‑1 (MQ=60)
                                                                                                 TAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGT                                                     <  SRR19973080.844867/150‑1 (MQ=60)
                                                                                                 TAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGT                                                     <  SRR19973080.1573336/150‑1 (MQ=60)
                                                                                                 TAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGT                                                     <  SRR19973080.282687/150‑1 (MQ=60)
                                                                                                  AAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTG                                                    >  SRR19973080.1155888/1‑150 (MQ=60)
                                                                                                  AAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTG                                                    >  SRR19973080.219710/1‑150 (MQ=60)
                                                                                                    AACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGAC                                                  >  SRR19973080.1496100/1‑150 (MQ=60)
                                                                                                     ACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACT                                                 >  SRR19973080.3959965/1‑150 (MQ=60)
                                                                                                     ACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACT                                                 <  SRR19973080.3812276/150‑1 (MQ=60)
                                                                                                     ACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACT                                                 <  SRR19973080.3812115/150‑1 (MQ=60)
                                                                                                         TGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAA                                             >  SRR19973080.1622860/1‑150 (MQ=60)
                                                                                                          GCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAA                                            >  SRR19973080.2372519/1‑150 (MQ=60)
                                                                                                          GCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAA                                            >  SRR19973080.47547/1‑150 (MQ=60)
                                                                                                             AATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATA                                         >  SRR19973080.194801/1‑150 (MQ=60)
                                                                                                             AATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATA                                         >  SRR19973080.3357522/1‑150 (MQ=60)
                                                                                                             AATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATA                                         >  SRR19973080.3428851/1‑150 (MQ=60)
                                                                                                             AATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATA                                         >  SRR19973080.2108508/1‑150 (MQ=60)
                                                                                                             AATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATA                                         >  SRR19973080.3430200/1‑150 (MQ=60)
                                                                                                              ATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAG                                        <  SRR19973080.4071295/150‑1 (MQ=60)
                                                                                                              AGTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAG                                        <  SRR19973080.4071226/150‑1 (MQ=60)
                                                                                                               TTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGT                                       <  SRR19973080.2007863/150‑1 (MQ=60)
                                                                                                                TCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTG                                      >  SRR19973080.3637740/1‑150 (MQ=60)
                                                                                                                TCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTG                                      <  SRR19973080.207138/150‑1 (MQ=60)
                                                                                                                TCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTG                                      <  SRR19973080.207016/150‑1 (MQ=60)
                                                                                                                 CCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGA                                     <  SRR19973080.2396793/150‑1 (MQ=60)
                                                                                                                     TCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTC                                 <  SRR19973080.3727094/150‑1 (MQ=60)
                                                                                                                     TCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTC                                 <  SRR19973080.3726535/150‑1 (MQ=60)
                                                                                                                       GAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGC                               >  SRR19973080.3734720/1‑150 (MQ=60)
                                                                                                                       GAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGC                               >  SRR19973080.2926095/1‑150 (MQ=60)
                                                                                                                       GAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACATCGC                               >  SRR19973080.3056164/1‑150 (MQ=60)
                                                                                                                        AAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCG                              >  SRR19973080.2621439/1‑150 (MQ=60)
                                                                                                                         AAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGC                             >  SRR19973080.4548039/1‑150 (MQ=60)
                                                                                                                          AATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCA                            >  SRR19973080.1316792/1‑150 (MQ=60)
                                                                                                                           ATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAA                           >  SRR19973080.2837124/1‑150 (MQ=60)
                                                                                                                             AAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAA                         >  SRR19973080.2952562/1‑150 (MQ=60)
                                                                                                                             AAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTAACCTCGCGCAAAA                         >  SRR19973080.2951475/1‑150 (MQ=60)
                                                                                                                              AACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAAT                        <  SRR19973080.432958/150‑1 (MQ=60)
                                                                                                                              AACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAAT                        <  SRR19973080.432949/150‑1 (MQ=60)
                                                                                                                              AACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAAT                        <  SRR19973080.1533683/150‑1 (MQ=60)
                                                                                                                                    ATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTA                  >  SRR19973080.3618024/1‑150 (MQ=60)
                                                                                                                                      CTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAAT                >  SRR19973080.2731149/1‑150 (MQ=60)
                                                                                                                                      CTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAAT                <  SRR19973080.96756/150‑1 (MQ=60)
                                                                                                                                      CTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAAT                <  SRR19973080.96524/150‑1 (MQ=60)
                                                                                                                                      CTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAAT                >  SRR19973080.453485/1‑150 (MQ=60)
                                                                                                                                       TGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATA               >  SRR19973080.2823562/1‑150 (MQ=60)
                                                                                                                                       TGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATA               >  SRR19973080.2823559/1‑150 (MQ=60)
                                                                                                                                           AGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGACGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAA           >  SRR19973080.4074439/1‑150 (MQ=60)
                                                                                                                                           AGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAA           >  SRR19973080.4074576/1‑150 (MQ=60)
                                                                                                                                            GAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAAC          <  SRR19973080.3208858/150‑1 (MQ=60)
                                                                                                                                              GATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAG        >  SRR19973080.803857/1‑150 (MQ=60)
                                                                                                                                               ATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGG       >  SRR19973080.3023527/1‑150 (MQ=60)
                                                                                                                                                  CCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCT    >  SRR19973080.3291101/1‑150 (MQ=60)
                                                                                                                                                  CCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCT    >  SRR19973080.3489233/1‑150 (MQ=60)
                                                                                                                                                   CTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTG   <  SRR19973080.4186753/150‑1 (MQ=60)
                                                                                                                                                    TTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGG  >  SRR19973080.3502715/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
CCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGG  >  CP009273/456439‑456736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑