Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F159 I0 R1	879	305.9	9588012	96.6%	9262019	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	456,588	G→T	93.3%	D749Y (GAC→TAC)	lon →	DNA‑binding ATP‑dependent protease La

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	456,588	0	G	T	93.3%	753.2 / 40.3	237	D749Y (GAC→TAC)	lon	DNA‑binding ATP‑dependent protease La
Reads supporting (aligned to +/- strand): ref base G (10/6); new base T (112/109); total (122/115)
Fisher's exact test for biased strand distribution p-value = 4.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 237 total aligned reads displayed.

GATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCT  >  CP009273/456441‑456734

gATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTAc                                                                                                                                                  >  2:1204768/1‑150 (MQ=255)

    tgGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACg                                                                                                                                              <  2:354803/150‑1 (MQ=255)

     ggCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGt                                                                                                                                             <  2:1403148/150‑1 (MQ=255)

       cAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTaa                                                                                                                                           <  2:641199/150‑1 (MQ=255)

       cAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTaa                                                                                                                                           >  2:630154/1‑150 (MQ=255)

        aaTGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGGAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAAt                                                                                                                                          >  1:1226663/1‑150 (MQ=255)

         aTGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAAtt                                                                                                                                         <  1:3488677/150‑1 (MQ=255)

           gACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGc                                                                                                                                       >  1:1035694/1‑150 (MQ=255)

            aCCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGcc                                                                                                                                      <  1:2925726/150‑1 (MQ=255)

            aCCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGcc                                                                                                                                      <  1:2805248/150‑1 (MQ=255)

            aCCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGAGTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGcc                                                                                                                                      >  2:3624083/1‑150 (MQ=255)

                  gagaTCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTg                                                                                                                                <  1:1365634/150‑1 (MQ=255)

                  gagaTCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTg                                                                                                                                <  1:1254473/150‑1 (MQ=255)

                    gaTCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGa                                                                                                                              <  1:820119/150‑1 (MQ=255)

                    gaTCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGa                                                                                                                              >  2:4787568/1‑150 (MQ=255)

                      tCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACa                                                                                                                            <  1:4674167/150‑1 (MQ=255)

                        aCTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACAtt                                                                                                                          >  2:1991174/1‑150 (MQ=255)

                         ctctGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTc                                                                                                                         <  1:1773171/150‑1 (MQ=255)

                               gTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCtg                                                                                                                   >  1:2755255/1‑150 (MQ=255)

                               gTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCtg                                                                                                                   <  1:1090440/150‑1 (MQ=255)

                               gTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCtg                                                                                                                   >  1:242073/1‑150 (MQ=255)

                                  gTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGa                                                                                                                <  1:2621436/150‑1 (MQ=255)

                                    cAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAg                                                                                                              >  1:346955/1‑150 (MQ=255)

                                    cAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAg                                                                                                              >  1:2494849/1‑150 (MQ=255)

                                    cAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAg                                                                                                              >  2:1458127/1‑150 (MQ=255)

                                      ggTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAgcg                                                                                                            <  1:1897201/150‑1 (MQ=255)

                                          cTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCAtt                                                                                                        <  1:3848846/150‑1 (MQ=255)

                                             ccGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAg                                                                                                     <  1:3245198/150‑1 (MQ=255)

                                               gATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGa                                                                                                   <  2:3071878/150‑1 (MQ=255)

                                                aTCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGaa                                                                                                  >  1:3338348/1‑150 (MQ=255)

                                                     tggtTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCt                                                                                             <  2:4690138/150‑1 (MQ=255)

                                                      ggtTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTg                                                                                            >  2:2468699/1‑150 (MQ=255)

                                                       gtTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGa                                                                                           <  2:1727766/150‑1 (MQ=255)

                                                        tttGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGAc                                                                                          <  1:3314125/150‑1 (MQ=255)

                                                          tGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGActc                                                                                        >  1:2771751/1‑150 (MQ=255)

                                                            aaagaaaAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTg                                                                                      <  1:2334457/150‑1 (MQ=255)

                                                            aaagaaaAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTg                                                                                      <  1:2334752/150‑1 (MQ=255)

                                                            aaagaaaAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTg                                                                                      >  1:3480685/1‑150 (MQ=255)

                                                             aagaaaAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTgg                                                                                     <  2:2755255/150‑1 (MQ=255)

                                                              agaaaAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGgc                                                                                    <  2:1983807/150‑1 (MQ=255)

                                                                 aaaaCTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCt                                                                                 <  1:3650319/150‑1 (MQ=255)

                                                                 aaaaCTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCt                                                                                 <  1:2715268/150‑1 (MQ=255)

                                                                 aaaaCTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCt                                                                                 <  1:2717258/150‑1 (MQ=255)

                                                                       ccTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCaaaa                                                                           >  1:3905733/1‑150 (MQ=255)

                                                                       ccTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCaaaa                                                                           <  1:436051/150‑1 (MQ=255)

                                                                        cTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAAt                                                                          >  2:4248578/1‑150 (MQ=255)

                                                                         tGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATg                                                                         <  2:1161433/150‑1 (MQ=255)

                                                                             aGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAAcc                                                                     <  2:2913293/150‑1 (MQ=255)

                                                                             aGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAAcc                                                                     <  1:985714/150‑1 (MQ=255)

                                                                             aGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAAcc                                                                     <  1:2530267/150‑1 (MQ=255)

                                                                               cgcATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGt                                                                   >  2:4405162/1‑150 (MQ=255)

                                                                                 cATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCt                                                                 >  1:301789/1‑150 (MQ=255)

                                                                                  aTCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTg                                                                <  2:3099161/150‑1 (MQ=255)

                                                                                   tCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTgg                                                               <  2:1904975/150‑1 (MQ=255)

                                                                                    cgcgGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGt                                                              >  2:1719522/1‑150 (MQ=255)

                                                                                       ggcggGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATg                                                           >  1:2235921/1‑150 (MQ=255)

                                                                                         cggGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCa                                                         >  1:3675069/1‑150 (MQ=255)

                                                                                         cggGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCa                                                         >  1:3981684/1‑150 (MQ=255)

                                                                                             aTTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGtt                                                     >  1:4575009/1‑150 (MQ=255)

                                                                                               tAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTtgt                                                   >  2:287667/1‑150 (MQ=255)

                                                                                                aaaaCAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTtgtg                                                  >  2:2159951/1‑150 (MQ=255)

                                                                                                 aaaCAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGa                                                 <  2:3100578/150‑1 (MQ=255)

                                                                                                  aaCAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGAc                                                >  1:314653/1‑150 (MQ=255)

                                                                                                    cAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGTAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGTAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTTTGACTg                                              <  1:2960299/150‑1 (MQ=255)

                                                                                                     aGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGAccga                                             >  1:2460677/1‑147 (MQ=255)

                                                                                                       tGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCaa                                           >  2:3303320/1‑150 (MQ=255)

                                                                                                        gCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCaaa                                          >  1:1944152/1‑150 (MQ=255)

                                                                                                         cTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCaaaa                                         >  2:231131/1‑150 (MQ=255)

                                                                                                         cTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCaaaa                                         >  1:165286/1‑150 (MQ=255)

                                                                                                          tAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAAt                                        >  1:218479/1‑150 (MQ=255)

                                                                                                          tAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAAt                                        >  2:3823263/1‑150 (MQ=255)

                                                                                                          tAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAAt                                        >  2:2628572/1‑150 (MQ=255)

                                                                                                              tCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTg                                    >  1:30973/1‑150 (MQ=255)

                                                                                                                cGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGAc                                  <  2:1856055/150‑1 (MQ=255)

                                                                                                                cGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGAc                                  >  1:1433716/1‑150 (MQ=255)

                                                                                                                 gTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGAcc                                 <  2:2061057/150‑1 (MQ=255)

                                                                                                                   tCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTc                               <  2:2798703/150‑1 (MQ=255)

                                                                                                                   tCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTc                               <  2:176851/150‑1 (MQ=255)

                                                                                                                    cGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTcg                              >  2:4019092/1‑150 (MQ=255)

                                                                                                                    cGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTcg                              <  1:3868443/150‑1 (MQ=255)

                                                                                                                    cGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTcg                              <  2:3444359/150‑1 (MQ=255)

                                                                                                                    cGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTcg                              >  1:802852/1‑150 (MQ=255)

                                                                                                                    cGAAAATAAACGCGATCTGGAAGAGAGTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTcg                              <  1:585471/150‑1 (MQ=255)

                                                                                                                     gAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTcgc                             <  2:2495520/150‑1 (MQ=255)

                                                                                                                     gAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTcgc                             >  2:4047674/1‑150 (MQ=255)

                                                                                                                      aaaaTAAACGCGCTCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTcgcg                            <  1:1599813/150‑1 (MQ=255)

                                                                                                                       aaaTAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCgcgc                           >  2:255602/1‑150 (MQ=255)

                                                                                                                           aaaCGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCaaaa                       >  2:3630910/1‑150 (MQ=255)

                                                                                                                           aaaCGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCaaaa                       >  2:4141263/1‑150 (MQ=255)

                                                                                                                              cgcgATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGc                    >  1:1779339/1‑150 (MQ=255)

                                                                                                                                cgATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCAc                  >  1:4773935/1‑150 (MQ=255)

                                                                                                                                  aTCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACta                >  1:2664730/1‑150 (MQ=255)

                                                                                                                                       gAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATaaa           >  2:751561/1‑150 (MQ=255)

                                                                                                                                         agagATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATaaaaa         <  1:529748/150‑1 (MQ=255)

                                                                                                                                          gagaTTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAAc        <  1:1577899/150‑1 (MQ=255)

                                                                                                                                            gaTTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAg      <  2:2981119/150‑1 (MQ=255)

                                                                                                                                            gaTTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAg      >  2:1260037/1‑150 (MQ=255)

                                                                                                                                            gaTTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAg      >  1:3569558/1‑150 (MQ=255)

                                                                                                                                              ttCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAggg    <  2:807505/150‑1 (MQ=255)

                                                                                                                                                ccTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCt  >  2:4317588/1‑150 (MQ=255)

GATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCT  >  CP009273/456441‑456734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTG  >  CP009273/456439‑456735

CCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTT                                                                                                                                                     <  SRR19973078.3938261/150‑1 (MQ=60)

     GTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAAC                                                                                                                                                <  SRR19973078.3491395/150‑1 (MQ=60)

        GCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTA                                                                                                                                             <  SRR19973078.1255963/150‑1 (MQ=60)

        GCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTA                                                                                                                                             <  SRR19973078.3683092/150‑1 (MQ=60)

        GCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTA                                                                                                                                             <  SRR19973078.1450822/150‑1 (MQ=60)

         CAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAA                                                                                                                                            >  SRR19973078.3167745/1‑150 (MQ=60)

          AATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGGAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAAT                                                                                                                                           >  SRR19973078.1234164/1‑150 (MQ=60)

           ATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATT                                                                                                                                          <  SRR19973078.3508523/150‑1 (MQ=60)

             GACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGC                                                                                                                                        >  SRR19973078.1042104/1‑150 (MQ=60)

              ACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCC                                                                                                                                       <  SRR19973078.2942418/150‑1 (MQ=60)

              ACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCC                                                                                                                                       <  SRR19973078.2223847/150‑1 (MQ=60)

              ACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCC                                                                                                                                       <  SRR19973078.2820849/150‑1 (MQ=60)

              ACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCC                                                                                                                                       <  SRR19973078.2820895/150‑1 (MQ=60)

               CCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCG                                                                                                                                      <  SRR19973078.3506875/150‑1 (MQ=60)

               CCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCG                                                                                                                                      <  SRR19973078.2079963/150‑1 (MQ=60)

               CCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCG                                                                                                                                      <  SRR19973078.3352398/150‑1 (MQ=60)

               CCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCG                                                                                                                                      <  SRR19973078.3988656/150‑1 (MQ=60)

                    GAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTG                                                                                                                                 >  SRR19973078.1046134/1‑150 (MQ=60)

                    GAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTG                                                                                                                                 <  SRR19973078.1262155/150‑1 (MQ=60)

                    GAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTG                                                                                                                                 <  SRR19973078.1262245/150‑1 (MQ=60)

                    GAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCGGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTG                                                                                                                                 <  SRR19973078.1374159/150‑1 (MQ=60)

                     AGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGG                                                                                                                                >  SRR19973078.2682628/1‑150 (MQ=60)

                      GATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGA                                                                                                                               <  SRR19973078.824933/150‑1 (MQ=60)

                        TCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACA                                                                                                                             <  SRR19973078.4700585/150‑1 (MQ=60)

                        TCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAAGTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACA                                                                                                                             <  SRR19973078.4700714/150‑1 (MQ=60)

                         CACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACAT                                                                                                                            >  SRR19973078.3196657/1‑150 (MQ=60)

                           CTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTC                                                                                                                          <  SRR19973078.1784077/150‑1 (MQ=60)

                           CTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTC                                                                                                                          <  SRR19973078.1783936/150‑1 (MQ=60)

                             CTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCAT                                                                                                                        >  SRR19973078.4395318/1‑150 (MQ=60)

                                CGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCT                                                                                                                     <  SRR19973078.3881363/150‑1 (MQ=60)

                                 GTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTG                                                                                                                    >  SRR19973078.243924/1‑150 (MQ=60)

                                 GTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTG                                                                                                                    >  SRR19973078.2770690/1‑150 (MQ=60)

                                 GTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTG                                                                                                                    >  SRR19973078.2770812/1‑150 (MQ=60)

                                 GTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTG                                                                                                                    <  SRR19973078.1097103/150‑1 (MQ=60)

                                  TGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGTAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGT                                                                                                                   <  SRR19973078.2334717/150‑1 (MQ=60)

                                    GTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGA                                                                                                                 <  SRR19973078.2636415/150‑1 (MQ=60)

                                    GTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGA                                                                                                                 <  SRR19973078.176454/150‑1 (MQ=60)

                                      CAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAG                                                                                                               >  SRR19973078.349160/1‑150 (MQ=60)

                                      CAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAG                                                                                                               >  SRR19973078.349145/1‑150 (MQ=60)

                                      CAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAG                                                                                                               >  SRR19973078.2509183/1‑150 (MQ=60)

                                       AGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGC                                                                                                              >  SRR19973078.726421/1‑150 (MQ=60)

                                       AGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGC                                                                                                              >  SRR19973078.726623/1‑150 (MQ=60)

                                        GGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCG                                                                                                             <  SRR19973078.1908451/150‑1 (MQ=60)

                                        GGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCG                                                                                                             <  SRR19973078.1908394/150‑1 (MQ=60)

                                         GTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGC                                                                                                            >  SRR19973078.2646933/1‑150 (MQ=60)

                                            CTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATT                                                                                                         <  SRR19973078.3870566/150‑1 (MQ=60)

                                            CTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATT                                                                                                         <  SRR19973078.3870873/150‑1 (MQ=60)

                                               CCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAG                                                                                                      <  SRR19973078.3263511/150‑1 (MQ=60)

                                                  ATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAA                                                                                                   >  SRR19973078.3357419/1‑150 (MQ=60)

                                                     GGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGACGTT                                                                                                >  SRR19973078.310860/1‑150 (MQ=60)

                                                       TGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAACTAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCT                                                                                              <  SRR19973078.2965403/150‑1 (MQ=60)

                                                          TTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGAC                                                                                           <  SRR19973078.3333046/150‑1 (MQ=60)

                                                           TTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAAGTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACT                                                                                          <  SRR19973078.3300610/150‑1 (MQ=60)

                                                            TGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTC                                                                                         <  SRR19973078.2607462/150‑1 (MQ=60)

                                                            TGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTC                                                                                         >  SRR19973078.2787228/1‑150 (MQ=60)

                                                             GAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCT                                                                                        <  SRR19973078.2736785/150‑1 (MQ=60)

                                                              AAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTG                                                                                       >  SRR19973078.3500742/1‑150 (MQ=60)

                                                              AAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTG                                                                                       >  SRR19973078.3500504/1‑150 (MQ=60)

                                                              AAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTG                                                                                       <  SRR19973078.2347751/150‑1 (MQ=60)

                                                              AAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTG                                                                                       >  SRR19973078.812161/1‑150 (MQ=60)

                                                              AAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTG                                                                                       <  SRR19973078.2348046/150‑1 (MQ=60)

                                                              AAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTG                                                                                       <  SRR19973078.2348286/150‑1 (MQ=60)

                                                              AAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAAGTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTG                                                                                       <  SRR19973078.2347811/150‑1 (MQ=60)

                                                               AAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGG                                                                                      >  SRR19973078.3897001/1‑150 (MQ=60)

                                                               AAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGG                                                                                      >  SRR19973078.3896994/1‑150 (MQ=60)

                                                               AAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGG                                                                                      >  SRR19973078.2874738/1‑150 (MQ=60)

                                                                  AAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGC                                                                                   >  SRR19973078.2656649/1‑150 (MQ=60)

                                                                  AAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGC                                                                                   >  SRR19973078.254371/1‑150 (MQ=60)

                                                                   AAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCT                                                                                  <  SRR19973078.2732580/150‑1 (MQ=60)

                                                                   AAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCT                                                                                  <  SRR19973078.3671128/150‑1 (MQ=60)

                                                                   AAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCT                                                                                  <  SRR19973078.3671108/150‑1 (MQ=60)

                                                                   AAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCT                                                                                  <  SRR19973078.2730594/150‑1 (MQ=60)

                                                                   AAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCT                                                                                  <  SRR19973078.2732587/150‑1 (MQ=60)

                                                                         CCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAA                                                                            >  SRR19973078.3927968/1‑150 (MQ=60)

                                                                         CCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAA                                                                            <  SRR19973078.438779/150‑1 (MQ=60)

                                                                           TGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATG                                                                          >  SRR19973078.175038/1‑150 (MQ=60)

                                                                               AGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACC                                                                      <  SRR19973078.991878/150‑1 (MQ=60)

                                                                               AGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACC                                                                      <  SRR19973078.2544784/150‑1 (MQ=60)

                                                                                   CATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCT                                                                  >  SRR19973078.303856/1‑150 (MQ=60)

                                                                                   CATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCT                                                                  >  SRR19973078.302816/1‑150 (MQ=60)

                                                                                   CATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCT                                                                  >  SRR19973078.302645/1‑150 (MQ=60)

                                                                                         GGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATG                                                            >  SRR19973078.2248924/1‑150 (MQ=60)

                                                                                           CGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCA                                                          >  SRR19973078.3696062/1‑150 (MQ=60)

                                                                                           CGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCA                                                          >  SRR19973078.4340379/1‑150 (MQ=60)

                                                                                           CGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCA                                                          >  SRR19973078.4004149/1‑150 (MQ=60)

                                                                                             GGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGG                                                        >  SRR19973078.3130282/1‑150 (MQ=60)

                                                                                              GATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGT                                                       >  SRR19973078.1930402/1‑150 (MQ=60)

                                                                                               ATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTT                                                      >  SRR19973078.4600983/1‑150 (MQ=60)

                                                                                               ATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTT                                                      >  SRR19973078.4600944/1‑150 (MQ=60)

                                                                                                 TAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGT                                                    >  SRR19973078.2927125/1‑150 (MQ=60)

                                                                                                  AAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTG                                                   >  SRR19973078.4099908/1‑150 (MQ=60)

                                                                                                    AACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGAC                                                 >  SRR19973078.316766/1‑150 (MQ=60)

                                                                                                      CAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGTAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGTAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTTTGACTG                                               <  SRR19973078.2977193/150‑1 (MQ=60)

                                                                                                      CAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTG                                               >  SRR19973078.133046/1‑150 (MQ=60)

                                                                                                       AGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACcga                                              >  SRR19973078.2474795/1‑147 (MQ=60)

                                                                                                       AGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACcga                                              >  SRR19973078.2474774/1‑147 (MQ=60)

                                                                                                       AGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACcga                                              >  SRR19973078.2474694/1‑147 (MQ=60)

                                                                                                          GCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAA                                           >  SRR19973078.1955524/1‑150 (MQ=60)

                                                                                                           CTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAA                                          >  SRR19973078.166718/1‑150 (MQ=60)

                                                                                                           CTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAA                                          >  SRR19973078.858599/1‑150 (MQ=60)

                                                                                                           CTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAA                                          >  SRR19973078.166723/1‑150 (MQ=60)

                                                                                                            TAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAAT                                         >  SRR19973078.220227/1‑150 (MQ=60)

                                                                                                            TAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAAT                                         >  SRR19973078.220223/1‑150 (MQ=60)

                                                                                                                TCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTG                                     >  SRR19973078.4720055/1‑150 (MQ=60)

                                                                                                                TCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTG                                     >  SRR19973078.31334/1‑150 (MQ=60)

                                                                                                                  CGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGAC                                   >  SRR19973078.1442561/1‑150 (MQ=60)

                                                                                                                     TCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTC                                <  SRR19973078.1429822/150‑1 (MQ=60)

                                                                                                                      CGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCG                               <  SRR19973078.3890540/150‑1 (MQ=60)

                                                                                                                      CGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCG                               <  SRR19973078.1467074/150‑1 (MQ=60)

                                                                                                                      CGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCG                               >  SRR19973078.807496/1‑150 (MQ=60)

                                                                                                                      CGAAAATAAACGCGATCTGGAAGAGAGTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCG                               <  SRR19973078.589332/150‑1 (MQ=60)

                                                                                                                        AAAATAAACGCGCTCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCG                             <  SRR19973078.1609325/150‑1 (MQ=60)

                                                                                                                        AAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCG                             <  SRR19973078.529048/150‑1 (MQ=60)

                                                                                                                                CGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGC                     >  SRR19973078.1790134/1‑150 (MQ=60)

                                                                                                                                CGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGC                     <  SRR19973078.3564890/150‑1 (MQ=60)

                                                                                                                                  CGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCAC                   >  SRR19973078.1794510/1‑150 (MQ=60)

                                                                                                                                  CGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCAC                   >  SRR19973078.4800686/1‑150 (MQ=60)

                                                                                                                                  CGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCAC                   >  SRR19973078.2485305/1‑150 (MQ=60)

                                                                                                                                   GATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACT                  <  SRR19973078.1595081/150‑1 (MQ=60)

                                                                                                                                    ATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTA                 >  SRR19973078.2679900/1‑150 (MQ=60)

                                                                                                                                     TCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAA                <  SRR19973078.3480289/150‑1 (MQ=60)

                                                                                                                                       TGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATA              >  SRR19973078.2425034/1‑150 (MQ=60)

                                                                                                                                        GGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAA             >  SRR19973078.1176586/1‑150 (MQ=60)

                                                                                                                                          AAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAA           <  SRR19973078.552320/150‑1 (MQ=60)

                                                                                                                                           AGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAA          <  SRR19973078.533248/150‑1 (MQ=60)

                                                                                                                                            GAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAAC         <  SRR19973078.1587249/150‑1 (MQ=60)

                                                                                                                                            GAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAAC         <  SRR19973078.2763082/150‑1 (MQ=60)

                                                                                                                                              GATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAG       >  SRR19973078.3589649/1‑150 (MQ=60)

                                                                                                                                              GATTCCTGACAACGTAATTGCCGATCTGGACATTCATCATGTGAAGCGCATTGATGAAGTTCTGACTCTGGCGCTGCAAAAAGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAG       >  SRR19973078.3590548/1‑150 (MQ=60)

                                                                                                                                                   CTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTG  <  SRR19973078.4797482/150‑1 (MQ=60)

CCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTG  >  CP009273/456439‑456735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`