Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 456,588 G→T 90.1% D749Y (GAC→TAC)  lon → DNA‑binding ATP‑dependent protease La

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273456,5880GT90.1% 693.4 / 68.2 243D749Y (GAC→TAC) lonDNA‑binding ATP‑dependent protease La
Reads supporting (aligned to +/- strand):  ref base G (20/4);  new base T (108/111);  total (128/115)
Fisher's exact test for biased strand distribution p-value = 2.00e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-01

Only 100 of 243 total aligned reads displayed.

GATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTG  >  CP009273/456441‑456735
                                                                                                                                                   |                                                                                                                                                   
 aTGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACa                                                                                                                                                  >  1:1060377/1‑150 (MQ=255)
 aTGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACa                                                                                                                                                  <  1:372714/150‑1 (MQ=255)
 aTGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACa                                                                                                                                                  >  1:3842599/1‑150 (MQ=255)
 aTGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACa                                                                                                                                                  <  2:4144984/150‑1 (MQ=255)
   gtgGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAAc                                                                                                                                                >  1:793298/1‑150 (MQ=255)
      gCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTa                                                                                                                                             <  2:1042774/150‑1 (MQ=255)
         aTGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAAtt                                                                                                                                          <  1:1116891/150‑1 (MQ=255)
            aCCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGcc                                                                                                                                       <  1:2877125/150‑1 (MQ=255)
            aCCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGcc                                                                                                                                       <  1:1737802/150‑1 (MQ=255)
             ccGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCg                                                                                                                                      <  2:1723038/150‑1 (MQ=255)
                 tGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCt                                                                                                                                  <  1:3200317/150‑1 (MQ=255)
                 tGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCt                                                                                                                                  <  1:3932802/150‑1 (MQ=255)
                 tGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCt                                                                                                                                  <  1:1160297/150‑1 (MQ=255)
                   agaTCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTgg                                                                                                                                >  2:3722974/1‑150 (MQ=255)
                   agaTCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTgg                                                                                                                                <  2:3470859/150‑1 (MQ=255)
                    gaTCACTCTGCGTGGTCAGGTACTGCCGCTCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGa                                                                                                                               <  2:1432323/150‑1 (MQ=255)
                     aTCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGAc                                                                                                                              >  2:794288/1‑150 (MQ=255)
                      tCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACa                                                                                                                             <  2:498092/150‑1 (MQ=255)
                       cACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACAt                                                                                                                            >  2:3035909/1‑150 (MQ=255)
                          tctGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAACAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCa                                                                                                                         <  2:3902281/150‑1 (MQ=255)
                           ctGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCAt                                                                                                                        <  2:1667060/150‑1 (MQ=255)
                            tGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATc                                                                                                                       <  1:3298918/150‑1 (MQ=255)
                            tGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATc                                                                                                                       <  1:1911061/150‑1 (MQ=255)
                              cGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCt                                                                                                                     <  2:1444195/150‑1 (MQ=255)
                                  gTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGa                                                                                                                 <  2:1124692/150‑1 (MQ=255)
                                  gTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGa                                                                                                                 <  2:3543674/150‑1 (MQ=255)
                                   tCAGGTACTGCCGATGGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTACTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGaa                                                                                                                <  1:1326128/150‑1 (MQ=255)
                                     aGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAgc                                                                                                              <  2:253090/150‑1 (MQ=255)
                                       gTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAgcgc                                                                                                            >  2:1750645/1‑150 (MQ=255)
                                        tACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCa                                                                                                           >  1:2791359/1‑150 (MQ=255)
                                          cTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCAtt                                                                                                         <  2:950106/150‑1 (MQ=255)
                                            gCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGa                                                                                                       >  2:4046450/1‑150 (MQ=255)
                                               gATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGa                                                                                                    <  1:416035/150‑1 (MQ=255)
                                               gATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGa                                                                                                    <  1:416042/150‑1 (MQ=255)
                                               gATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGa                                                                                                    <  1:416090/150‑1 (MQ=255)
                                                 tCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAg                                                                                                  <  1:1938132/150‑1 (MQ=255)
                                                   ggtggtTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGtt                                                                                                <  1:1436930/150‑1 (MQ=255)
                                                     tggtTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCt                                                                                              <  1:3145555/150‑1 (MQ=255)
                                                      ggtTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTg                                                                                             <  1:2049191/150‑1 (MQ=255)
                                                      ggtTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTg                                                                                             <  1:2563601/150‑1 (MQ=255)
                                                       gtTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGa                                                                                            >  2:3165850/1‑150 (MQ=255)
                                                        tttGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGAc                                                                                           >  1:3475056/1‑150 (MQ=255)
                                                         ttGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGAct                                                                                          <  2:71781/150‑1 (MQ=255)
                                                         ttGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGAct                                                                                          <  2:2248011/150‑1 (MQ=255)
                                                         ttGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGAct                                                                                          >  2:3138645/1‑150 (MQ=255)
                                                          tGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGActc                                                                                         >  2:3762256/1‑150 (MQ=255)
                                                            aaagaaaAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTg                                                                                       >  1:430430/1‑150 (MQ=255)
                                                            aaagaaaAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTg                                                                                       >  1:1526368/1‑150 (MQ=255)
                                                                 aaaaCTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCt                                                                                  <  2:2287337/150‑1 (MQ=255)
                                                                    aCTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCa                                                                               >  1:3249750/1‑150 (MQ=255)
                                                                       ccTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCaaaa                                                                            <  1:4227923/150‑1 (MQ=255)
                                                                       ccTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCaaaa                                                                            >  2:3691915/1‑150 (MQ=255)
                                                                       ccTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCAGTGAGGAAGTTCTGACTCTGGCGCTGCaaaa                                                                            >  2:2747112/1‑150 (MQ=255)
                                                                        cTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAAt                                                                           >  2:1265079/1‑150 (MQ=255)
                                                                        cTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAAt                                                                           >  1:4185651/1‑150 (MQ=255)
                                                                        cTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAAt                                                                           >  1:3612656/1‑150 (MQ=255)
                                                                         tGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGACGCGCCTTGAGGAAGTTCTGACTCTGGCGCTGCAAAATg                                                                          >  2:3736174/1‑150 (MQ=255)
                                                                           gCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGaa                                                                        >  2:2010415/1‑150 (MQ=255)
                                                                                 cATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCt                                                                  >  2:2272495/1‑150 (MQ=255)
                                                                                  aTCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTg                                                                 >  2:3636344/1‑150 (MQ=255)
                                                                                  aTCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTg                                                                 >  1:2635053/1‑150 (MQ=255)
                                                                                  aTCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTg                                                                 >  2:1535468/1‑150 (MQ=255)
                                                                                  aTCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTg                                                                 >  1:4013242/1‑150 (MQ=255)
                                                                                            gATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGt                                                       <  1:2104596/150‑1 (MQ=255)
                                                                                              ttAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTtg                                                     <  1:2094777/150‑1 (MQ=255)
                                                                                               tAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTtgt                                                    >  2:949719/1‑150 (MQ=255)
                                                                                               tAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTtgt                                                    <  1:718166/150‑1 (MQ=255)
                                                                                                  aaCAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGAc                                                 >  1:249366/1‑150 (MQ=255)
                                                                                                         cTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCaaaa                                          >  1:2957636/1‑150 (MQ=255)
                                                                                                          tAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAAt                                         >  2:2775594/1‑150 (MQ=255)
                                                                                                           aaTTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATa                                        >  1:4339016/1‑150 (MQ=255)
                                                                                                              tCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTg                                     >  1:515129/1‑150 (MQ=255)
                                                                                                                cGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCATGTTGTGACTGCAAAATAGTGAc                                   <  1:2400194/150‑1 (MQ=255)
                                                                                                                   tCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTc                                <  2:2033604/150‑1 (MQ=255)
                                                                                                                   tCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTc                                <  1:3035909/150‑1 (MQ=255)
                                                                                                                    cGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTcg                               <  1:467173/150‑1 (MQ=255)
                                                                                                                     gAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTcgc                              >  2:283260/1‑150 (MQ=255)
                                                                                                                     gAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTcgc                              >  1:846610/1‑150 (MQ=255)
                                                                                                                      aaaaTAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTcgcg                             >  1:2235455/1‑150 (MQ=255)
                                                                                                                       aaaTAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCgcgc                            >  2:2576633/1‑150 (MQ=255)
                                                                                                                       aaaTAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCgcgc                            >  2:4164110/1‑150 (MQ=255)
                                                                                                                        aaTAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCa                           >  2:1880517/1‑150 (MQ=255)
                                                                                                                            aaCGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAAt                       >  2:2972714/1‑150 (MQ=255)
                                                                                                                             aCGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATg                      >  1:1704754/1‑150 (MQ=255)
                                                                                                                                cgATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCAc                   >  1:765750/1‑150 (MQ=255)
                                                                                                                                cgATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCAc                   <  2:1242112/150‑1 (MQ=255)
                                                                                                                                cgATCTGGAAGAGATTCCGTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACGGCAAAATAGTGACCTCGCGCAAAATGCAc                   <  1:1672086/150‑1 (MQ=255)
                                                                                                                                 gATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACt                  >  2:2430507/1‑150 (MQ=255)
                                                                                                                                    cTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACtaat               >  2:10065/1‑150 (MQ=255)
                                                                                                                                       gAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATaaa            >  1:1395690/1‑150 (MQ=255)
                                                                                                                                       gAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATaaa            >  1:1481986/1‑150 (MQ=255)
                                                                                                                                        aaGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATaaaa           >  2:21850/1‑150 (MQ=255)
                                                                                                                                           agaTTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACa        >  1:1036793/1‑150 (MQ=255)
                                                                                                                                           agaTTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACa        <  2:812810/150‑1 (MQ=255)
                                                                                                                                           agaTTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACa        >  1:3596290/1‑150 (MQ=255)
                                                                                                                                            gaTTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAg       <  1:3636344/150‑1 (MQ=255)
                                                                                                                                             aTTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAgg      >  1:390182/1‑150 (MQ=255)
                                                                                                                                              ttCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAggg     <  1:83248/150‑1 (MQ=255)
                                                                                                                                                ccTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCt   >  1:3065852/1‑150 (MQ=255)
                                                                                                                                                 cTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTg  <  2:3920542/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTG  >  CP009273/456441‑456735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCT  >  CP009273/456440‑456734
                                                                                                                                                    |                                                                                                                                                  
CGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTA                                                                                                                                                   <  SRR19973079.321306/150‑1 (MQ=60)
CGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTA                                                                                                                                                   <  SRR19973079.3426017/150‑1 (MQ=60)
CGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTA                                                                                                                                                   <  SRR19973079.3424929/150‑1 (MQ=60)
 GATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGAC                                                                                                                                                  >  SRR19973079.2039767/1‑150 (MQ=60)
  ATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACA                                                                                                                                                 >  SRR19973079.1066179/1‑150 (MQ=60)
  ATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACA                                                                                                                                                 >  SRR19973079.3862215/1‑150 (MQ=60)
  ATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACA                                                                                                                                                 <  SRR19973079.374747/150‑1 (MQ=60)
    GTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAAC                                                                                                                                               >  SRR19973079.797804/1‑150 (MQ=60)
          ATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATT                                                                                                                                         <  SRR19973079.1123209/150‑1 (MQ=60)
           TGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTG                                                                                                                                        >  SRR19973079.4242430/1‑150 (MQ=60)
           TGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTG                                                                                                                                        <  SRR19973079.331342/150‑1 (MQ=60)
           TGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTG                                                                                                                                        <  SRR19973079.332210/150‑1 (MQ=60)
             ACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCC                                                                                                                                      <  SRR19973079.2891692/150‑1 (MQ=60)
             ACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCC                                                                                                                                      <  SRR19973079.1747016/150‑1 (MQ=60)
             ACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCC                                                                                                                                      <  SRR19973079.1747131/150‑1 (MQ=60)
                  TGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCT                                                                                                                                 <  SRR19973079.3952675/150‑1 (MQ=60)
                  TGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCT                                                                                                                                 <  SRR19973079.3216645/150‑1 (MQ=60)
                  TGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCT                                                                                                                                 <  SRR19973079.1166969/150‑1 (MQ=60)
                  TGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCT                                                                                                                                 <  SRR19973079.1166857/150‑1 (MQ=60)
                          CTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTC                                                                                                                         <  SRR19973079.3769134/150‑1 (MQ=60)
                             TGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATC                                                                                                                      <  SRR19973079.3315715/150‑1 (MQ=60)
                             TGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATC                                                                                                                      <  SRR19973079.3981273/150‑1 (MQ=60)
                             TGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATC                                                                                                                      <  SRR19973079.1921253/150‑1 (MQ=60)
                                GTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTG                                                                                                                   >  SRR19973079.3648204/1‑150 (MQ=60)
                                 TGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGT                                                                                                                  >  SRR19973079.3205746/1‑150 (MQ=60)
                                    TCAGGTACTGCCGATGGGTGGTTTGAAAGAAAATCTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTACTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAA                                                                                                               <  SRR19973079.1333597/150‑1 (MQ=60)
                                     CAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAG                                                                                                              >  SRR19973079.1927518/1‑150 (MQ=60)
                                       GGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCG                                                                                                            <  SRR19973079.3416004/150‑1 (MQ=60)
                                       GGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCG                                                                                                            >  SRR19973079.4138643/1‑150 (MQ=60)
                                         TACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCA                                                                                                          >  SRR19973079.2805679/1‑150 (MQ=60)
                                             GCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGA                                                                                                      >  SRR19973079.820618/1‑150 (MQ=60)
                                               CGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGTACATTCATCCTGTGAAGAGCATTGAGG                                                                                                    >  SRR19973079.493817/1‑150 (MQ=60)
                                               CGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGG                                                                                                    <  SRR19973079.3733174/150‑1 (MQ=60)
                                               CGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGG                                                                                                    >  SRR19973079.212738/1‑150 (MQ=60)
                                                GATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGA                                                                                                   <  SRR19973079.418474/150‑1 (MQ=60)
                                                GATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGA                                                                                                   <  SRR19973079.418679/150‑1 (MQ=60)
                                                GATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGA                                                                                                   <  SRR19973079.418527/150‑1 (MQ=60)
                                                GATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGA                                                                                                   <  SRR19973079.418496/150‑1 (MQ=60)
                                                GATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGA                                                                                                   <  SRR19973079.418479/150‑1 (MQ=60)
                                                GATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGA                                                                                                   <  SRR19973079.418471/150‑1 (MQ=60)
                                                GATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGA                                                                                                   <  SRR19973079.418478/150‑1 (MQ=60)
                                                  TCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAG                                                                                                 <  SRR19973079.1948470/150‑1 (MQ=60)
                                                    GGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTT                                                                                               <  SRR19973079.1444677/150‑1 (MQ=60)
                                                      TGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCT                                                                                             <  SRR19973079.3161710/150‑1 (MQ=60)
                                                       GGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTG                                                                                            <  SRR19973079.2059939/150‑1 (MQ=60)
                                                       GGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTG                                                                                            >  SRR19973079.1454352/1‑150 (MQ=60)
                                                       GGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTG                                                                                            <  SRR19973079.2576624/150‑1 (MQ=60)
                                                       GGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTG                                                                                            <  SRR19973079.2576652/150‑1 (MQ=60)
                                                        GTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGA                                                                                           >  SRR19973079.2601464/1‑150 (MQ=60)
                                                        GTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGA                                                                                           >  SRR19973079.4055580/1‑150 (MQ=60)
                                                         TTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGAC                                                                                          >  SRR19973079.3492966/1‑150 (MQ=60)
                                                          TTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACT                                                                                         <  SRR19973079.1426093/150‑1 (MQ=60)
                                                             AAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTG                                                                                      >  SRR19973079.432995/1‑150 (MQ=60)
                                                             AAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTG                                                                                      >  SRR19973079.1534782/1‑150 (MQ=60)
                                                                  AAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCT                                                                                 >  SRR19973079.1483770/1‑150 (MQ=60)
                                                                  AAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCT                                                                                 <  SRR19973079.1184600/150‑1 (MQ=60)
                                                                  AAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCT                                                                                 >  SRR19973079.431812/1‑150 (MQ=60)
                                                                     ACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCA                                                                              >  SRR19973079.3266175/1‑150 (MQ=60)
                                                                      CTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAA                                                                             >  SRR19973079.2355632/1‑150 (MQ=60)
                                                                        CCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAA                                                                           <  SRR19973079.4249315/150‑1 (MQ=60)
                                                                         CTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAAT                                                                          >  SRR19973079.1656468/1‑150 (MQ=60)
                                                                         CTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAAT                                                                          >  SRR19973079.3144037/1‑150 (MQ=60)
                                                                         CTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAAT                                                                          >  SRR19973079.4206869/1‑150 (MQ=60)
                                                                         CTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAAT                                                                          >  SRR19973079.1089340/1‑150 (MQ=60)
                                                                         CTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAAT                                                                          >  SRR19973079.3630963/1‑150 (MQ=60)
                                                                          TGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATG                                                                         <  SRR19973079.3877295/150‑1 (MQ=60)
                                                                          TGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATG                                                                         >  SRR19973079.2435576/1‑150 (MQ=60)
                                                                            GCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAA                                                                       >  SRR19973079.3940382/1‑150 (MQ=60)
                                                                            GCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAA                                                                       >  SRR19973079.3940262/1‑150 (MQ=60)
                                                                              AGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACC                                                                     >  SRR19973079.989334/1‑150 (MQ=60)
                                                                                   ATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTG                                                                >  SRR19973079.4033450/1‑150 (MQ=60)
                                                                                   ATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTG                                                                >  SRR19973079.2648664/1‑150 (MQ=60)
                                                                                             GATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGT                                                      <  SRR19973079.4311827/150‑1 (MQ=60)
                                                                                             GATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGT                                                      <  SRR19973079.2115529/150‑1 (MQ=60)
                                                                                               TTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTG                                                    <  SRR19973079.2105680/150‑1 (MQ=60)
                                                                                               TTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTG                                                    <  SRR19973079.2107056/150‑1 (MQ=60)
                                                                                                TAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGT                                                   <  SRR19973079.722003/150‑1 (MQ=60)
                                                                                                TAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGT                                                   <  SRR19973079.2844881/150‑1 (MQ=60)
                                                                                                  AAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGA                                                 >  SRR19973079.2589817/1‑150 (MQ=60)
                                                                                                   AACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGAC                                                >  SRR19973079.250995/1‑150 (MQ=60)
                                                                                                         GCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAA                                          >  SRR19973079.355090/1‑150 (MQ=60)
                                                                                                          CTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAA                                         >  SRR19973079.2972774/1‑150 (MQ=60)
                                                                                                          CTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAA                                         >  SRR19973079.2972805/1‑150 (MQ=60)
                                                                                                          CTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAA                                         >  SRR19973079.2972815/1‑150 (MQ=60)
                                                                                                            TATTCCGTTCGTAAATAAAAGCGATCTGGAAGAGATTCCTTACAACGTATTTGCCGCTCTGGACATTCATCCTGTGTATCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAATTTGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATA                                       <  SRR19973079.1838698/150‑1 (MQ=60)
                                                                                                            AATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATA                                       >  SRR19973079.4360794/1‑150 (MQ=60)
                                                                                                            AATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATA                                       >  SRR19973079.4360798/1‑150 (MQ=60)
                                                                                                               TCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTG                                    >  SRR19973079.518238/1‑150 (MQ=60)
                                                                                                                CCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGA                                   <  SRR19973079.3616454/150‑1 (MQ=60)
                                                                                                                CCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGA                                   <  SRR19973079.3617145/150‑1 (MQ=60)
                                                                                                                 CGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCATGTTGTGACTGCAAAATAGTGAC                                  <  SRR19973079.2412592/150‑1 (MQ=60)
                                                                                                                  GTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACC                                 >  SRR19973079.1139981/1‑150 (MQ=60)
                                                                                                                    TCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTC                               <  SRR19973079.3051624/150‑1 (MQ=60)
                                                                                                                     CGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCG                              <  SRR19973079.468118/150‑1 (MQ=60)
                                                                                                                     CGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCG                              <  SRR19973079.469992/150‑1 (MQ=60)
                                                                                                                      GAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGC                             >  SRR19973079.851510/1‑150 (MQ=60)
                                                                                                                      GAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGC                             >  SRR19973079.851506/1‑150 (MQ=60)
                                                                                                                       AAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCG                            >  SRR19973079.2247025/1‑150 (MQ=60)
                                                                                                                       AAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCG                            <  SRR19973079.298754/150‑1 (MQ=60)
                                                                                                                          ATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAA                         >  SRR19973079.60120/1‑150 (MQ=60)
                                                                                                                              ACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATG                     >  SRR19973079.1713881/1‑150 (MQ=60)
                                                                                                                              ACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATG                     >  SRR19973079.1713847/1‑150 (MQ=60)
                                                                                                                               CGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGC                    >  SRR19973079.1529005/1‑150 (MQ=60)
                                                                                                                                GCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCA                   <  SRR19973079.814628/150‑1 (MQ=60)
                                                                                                                                 CGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCACAATGCAC                  >  SRR19973079.769973/1‑150 (MQ=60)
                                                                                                                                 CGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCAC                  >  SRR19973079.770000/1‑150 (MQ=60)
                                                                                                                                 CGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCAC                  >  SRR19973079.770015/1‑150 (MQ=60)
                                                                                                                                 CGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCAC                  >  SRR19973079.536285/1‑150 (MQ=60)
                                                                                                                                 CGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCAC                  >  SRR19973079.4358676/1‑150 (MQ=60)
                                                                                                                                 CGATCTGGAAGAGATTCCGTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACGGCAAAATAGTGACCTCGCGCAAAATGCAC                  <  SRR19973079.1681114/150‑1 (MQ=60)
                                                                                                                                   ATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTA                >  SRR19973079.3202862/1‑150 (MQ=60)
                                                                                                                                     CTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAAT              <  SRR19973079.209327/150‑1 (MQ=60)
                                                                                                                                       GGAAGAGATTCCTTACACCGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAA            <  SRR19973079.3058628/150‑1 (MQ=60)
                                                                                                                                        GAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAA           >  SRR19973079.1490091/1‑150 (MQ=60)
                                                                                                                                        GAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAA           >  SRR19973079.3053772/1‑150 (MQ=60)
                                                                                                                                        GAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAA           >  SRR19973079.3688932/1‑150 (MQ=60)
                                                                                                                                        GAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAA           >  SRR19973079.1403353/1‑150 (MQ=60)
                                                                                                                                         AAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAA          <  SRR19973079.1728622/150‑1 (MQ=60)
                                                                                                                                         AAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAA          <  SRR19973079.2186190/150‑1 (MQ=60)
                                                                                                                                            AGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCACAATGCACTAATAAAAACA       >  SRR19973079.1042566/1‑150 (MQ=60)
                                                                                                                                            AGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACA       >  SRR19973079.273132/1‑150 (MQ=60)
                                                                                                                                            AGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACA       >  SRR19973079.1042542/1‑150 (MQ=60)
                                                                                                                                            AGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACA       >  SRR19973079.3614563/1‑150 (MQ=60)
                                                                                                                                            AGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACA       >  SRR19973079.2456983/1‑150 (MQ=60)
                                                                                                                                            AGAGTCCTTACCACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACC       <  SRR19973079.1954857/150‑1 (MQ=60)
                                                                                                                                             GATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAG      <  SRR19973079.3654766/150‑1 (MQ=60)
                                                                                                                                              ATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGG     >  SRR19973079.392417/1‑150 (MQ=60)
                                                                                                                                               TTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGG    <  SRR19973079.84017/150‑1 (MQ=60)
                                                                                                                                               TTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGG    <  SRR19973079.84047/150‑1 (MQ=60)
                                                                                                                                               TTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGAAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGG    >  SRR19973079.138650/1‑150 (MQ=60)
                                                                                                                                                 CCTTACAACGTAATTGCCGATCTTGACATTCATACTGTGAAGCGAATAGAGGAAGATCTGACTCTGGCGCTGCAAAATCAACAGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACAAATAAAAACAGGGCA  >  SRR19973079.168962/1‑150 (MQ=60)
                                                                                                                                                 CCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCT  >  SRR19973079.1355823/1‑150 (MQ=60)
                                                                                                                                                 CCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCT  >  SRR19973079.3081729/1‑150 (MQ=60)
                                                                                                                                                 CCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCT  <  SRR19973079.3231876/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                  
CGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCT  >  CP009273/456440‑456734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑