Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,439,518 C→A 66.8% S737S (TCC→TCA)  ydbH → putative membrane‑anchored protein, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,439,5180CA66.8% 242.2 / 274.9 235S737S (TCC→TCA) ydbHputative membrane‑anchored protein, function unknown
Reads supporting (aligned to +/- strand):  ref base C (39/39);  new base A (74/83);  total (113/122)
Fisher's exact test for biased strand distribution p-value = 7.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01

Only 100 of 238 total aligned reads displayed.

TTGACTGATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGCGGGTTC  >  CP009273/1439369‑1439667
                                                                                                                                                     |                                                                                                                                                     
ttGACTGATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTcc                                                                                                                                                       <  1:530015/150‑1 (MQ=255)
  gACTGATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAgg                                                                                                                                                     <  2:1277782/150‑1 (MQ=255)
   aCTGATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCggg                                                                                                                                                    <  2:1156871/150‑1 (MQ=255)
   aCTGATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCggg                                                                                                                                                    <  2:3445167/150‑1 (MQ=255)
          ttAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTa                                                                                                                                             <  2:530165/150‑1 (MQ=255)
             gCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAgtg                                                                                                                                          <  1:103933/150‑1 (MQ=255)
               gTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAgtggt                                                                                                                                        <  1:766632/150‑1 (MQ=255)
                 cGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGc                                                                                                                                      >  1:1303009/1‑150 (MQ=255)
                 cGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGc                                                                                                                                      >  1:2485674/1‑150 (MQ=255)
                    tgttTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACt                                                                                                                                   <  2:3632295/150‑1 (MQ=255)
                    tgtgTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACt                                                                                                                                   >  1:437989/1‑150 (MQ=255)
                       gtTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGcc                                                                                                                                <  1:571224/150‑1 (MQ=255)
                          aGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGtt                                                                                                                             <  2:2486000/150‑1 (MQ=255)
                          aGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGtt                                                                                                                             >  1:4448124/1‑150 (MQ=255)
                             cGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATg                                                                                                                          <  1:1909115/150‑1 (MQ=255)
                              ggTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATgg                                                                                                                         <  2:2931523/150‑1 (MQ=255)
                               gTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGc                                                                                                                        >  2:1747404/1‑150 (MQ=255)
                                tAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCt                                                                                                                       >  2:3406571/1‑150 (MQ=255)
                                   cGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGaa                                                                                                                    <  2:2404559/150‑1 (MQ=255)
                                    gTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAAc                                                                                                                   >  2:1050436/1‑150 (MQ=255)
                                    gTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAAc                                                                                                                   >  2:1865195/1‑150 (MQ=255)
                                       cTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAAt                                                                                                                >  1:1107963/1‑150 (MQ=255)
                                       cTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAAt                                                                                                                <  2:3853551/150‑1 (MQ=255)
                                         gatgaAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGa                                                                                                              >  2:2257758/1‑150 (MQ=255)
                                           tgaAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGaaa                                                                                                            <  2:1371027/150‑1 (MQ=255)
                                             aaaCAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGaaaaa                                                                                                          >  2:1899361/1‑150 (MQ=255)
                                              aaCAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAAt                                                                                                         <  2:2567684/150‑1 (MQ=255)
                                                 aaTTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGa                                                                                                      >  1:4062829/1‑150 (MQ=255)
                                                     aCGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGTACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATGTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATtgt                                                                                                  >  2:85605/1‑150 (MQ=255)
                                                     aCGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATtgt                                                                                                  >  2:466441/1‑150 (MQ=255)
                                                       gTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGa                                                                                                >  1:1449749/1‑150 (MQ=255)
                                                            ccGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGAt                                                                                           >  2:1887342/1‑150 (MQ=255)
                                                            ccGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGAt                                                                                           <  2:685981/150‑1 (MQ=255)
                                                              gCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGAtgg                                                                                         <  2:1824394/150‑1 (MQ=255)
                                                               cAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGAtggc                                                                                        <  1:3365952/150‑1 (MQ=255)
                                                               cAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGAtggc                                                                                        <  1:185622/150‑1 (MQ=255)
                                                               cAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGAtggc                                                                                        >  2:1485572/1‑150 (MQ=255)
                                                                  cATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGAtggctgg                                                                                     <  1:1393872/150‑1 (MQ=255)
                                                                  cATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAAATCGCCATGTCCTGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAATAtggctgg                                                                                     >  1:1701733/1‑150 (MQ=255)
                                                                   aTGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGAtggctggc                                                                                    >  2:2749330/1‑150 (MQ=255)
                                                                     gACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGAtggctggctg                                                                                  >  1:3717267/1‑150 (MQ=255)
                                                                     gACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGAtggctggctg                                                                                  <  1:3252756/150‑1 (MQ=255)
                                                                     gACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGAtggctggctg                                                                                  >  1:1082995/1‑150 (MQ=255)
                                                                          ggCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGaa                                                                             >  2:2395043/1‑150 (MQ=255)
                                                                          ggCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGaa                                                                             >  2:4159602/1‑150 (MQ=255)
                                                                            cgcTGTTGCGGCTGAATAATCTCTCATCCAGCGACCTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATa                                                                           >  2:1326791/1‑150 (MQ=255)
                                                                            cgcTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATa                                                                           <  2:2359133/150‑1 (MQ=255)
                                                                             gcTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAg                                                                          >  2:780827/1‑150 (MQ=255)
                                                                               tGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACTATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCg                                                                        <  2:2217525/150‑1 (MQ=255)
                                                                                gTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCgg                                                                       <  1:2558464/150‑1 (MQ=255)
                                                                                gTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCgg                                                                       >  2:50883/1‑150 (MQ=255)
                                                                                gTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCgg                                                                       >  2:2841874/1‑150 (MQ=255)
                                                                                   gCGGCTGAATAATCTCTCATCCAGCGATCTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGcc                                                                    <  1:4216430/150‑1 (MQ=255)
                                                                                   gCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGcc                                                                    <  1:2438908/150‑1 (MQ=255)
                                                                                    cGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCg                                                                   <  2:1074106/150‑1 (MQ=255)
                                                                                    cGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCg                                                                   <  2:3356345/150‑1 (MQ=255)
                                                                                      gCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGAt                                                                 <  1:3531965/150‑1 (MQ=255)
                                                                                       cTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATg                                                                >  2:2304556/1‑150 (MQ=255)
                                                                                        tGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGa                                                               >  1:3080209/1‑150 (MQ=255)
                                                                                        tGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGa                                                               <  1:3162538/150‑1 (MQ=255)
                                                                                            taatCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACAtt                                                           >  1:1035275/1‑150 (MQ=255)
                                                                                              ctctctcATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGc                                                         <  1:3443014/149‑1 (MQ=255)
                                                                                               tctctcATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCg                                                        <  1:811262/150‑1 (MQ=255)
                                                                                                ctctcATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGa                                                       >  2:4485513/1‑150 (MQ=255)
                                                                                                 tctcATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGAc                                                      >  2:2491070/1‑150 (MQ=255)
                                                                                                 tctcATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGAc                                                      >  1:2167086/1‑150 (MQ=255)
                                                                                                   tcATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTg                                                    >  1:3868722/1‑150 (MQ=255)
                                                                                                     aTCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGa                                                  >  1:1593870/1‑150 (MQ=255)
                                                                                                     aTCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGa                                                  >  2:3047466/1‑150 (MQ=255)
                                                                                                     aTCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGa                                                  <  1:3266540/150‑1 (MQ=255)
                                                                                                       ccAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATa                                                <  2:1299907/150‑1 (MQ=255)
                                                                                                       ccAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATa                                                <  1:1087789/150‑1 (MQ=255)
                                                                                                       ccAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATa                                                <  1:2171430/150‑1 (MQ=255)
                                                                                                       ccAGCGAACTGGTGAGCGCCGTCAATCCGAAACAAGTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATa                                                <  2:3305660/150‑1 (MQ=255)
                                                                                                        cAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATaa                                               <  1:1421564/150‑1 (MQ=255)
                                                                                                         aGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATaaa                                              >  1:3159663/1‑150 (MQ=255)
                                                                                                           cGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGACTAGCGGGCCGATGACATTGCGACTGGATAAAGa                                            >  2:1233471/1‑150 (MQ=255)
                                                                                                              aCTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGacac                                         >  1:2101309/1‑150 (MQ=255)
                                                                                                               cTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACt                                        <  1:3333924/150‑1 (MQ=255)
                                                                                                                  gTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCg                                     >  1:1524495/1‑150 (MQ=255)
                                                                                                                    tAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGa                                   <  2:4282273/150‑1 (MQ=255)
                                                                                                                           gTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCggtg                            >  2:2114162/1‑150 (MQ=255)
                                                                                                                           gTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCggtg                            <  2:704083/150‑1 (MQ=255)
                                                                                                                           gTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCggtg                            <  1:1033361/150‑1 (MQ=255)
                                                                                                                           gTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCggtg                            <  1:2894635/150‑1 (MQ=255)
                                                                                                                            tCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCggtgg                           <  1:698609/150‑1 (MQ=255)
                                                                                                                             cAATCCGAAACACTTCGCCATGTCAGGGGCATTGAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCggtggt                          <  2:1741552/150‑1 (MQ=255)
                                                                                                                             cAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACGGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCggtggt                          <  1:2661637/150‑1 (MQ=255)
                                                                                                                                   gAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGa                    <  2:322820/150‑1 (MQ=255)
                                                                                                                                       cAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAat                <  2:2910270/150‑1 (MQ=255)
                                                                                                                                         aTTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAatat              >  2:3983306/1‑150 (MQ=255)
                                                                                                                                         aTTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAatat              >  2:19654/1‑150 (MQ=255)
                                                                                                                                         aTTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAatat              <  1:4104648/150‑1 (MQ=255)
                                                                                                                                          ttCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATg             >  1:2272313/1‑150 (MQ=255)
                                                                                                                                          ttCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATg             <  1:2774313/150‑1 (MQ=255)
                                                                                                                                             gCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACt          >  2:3700291/1‑150 (MQ=255)
                                                                                                                                               cATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGc        >  1:1260699/1‑150 (MQ=255)
                                                                                                                                               cATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGc        <  2:4035439/150‑1 (MQ=255)
                                                                                                                                                aTGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGCg       >  1:3933596/1‑150 (MQ=255)
                                                                                                                                                     cGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGCGGGTTc  <  2:1216598/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTGACTGATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGCGGGTTC  >  CP009273/1439369‑1439667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTGACTGATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGCGGGTT  >  CP009273/1439369‑1439666
                                                                                                                                                     |                                                                                                                                                    
TTGACTGATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCC                                                                                                                                                      <  SRR19973080.533393/150‑1 (MQ=60)
TTGACTGATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCA                                                                                                                                                      >  SRR19973080.134471/1‑150 (MQ=60)
TTGACTGATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCA                                                                                                                                                      >  SRR19973080.2415510/1‑150 (MQ=60)
TTGACTGATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCA                                                                                                                                                      >  SRR19973080.1802080/1‑150 (MQ=60)
    CTGATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGG                                                                                                                                                  <  SRR19973080.2259449/150‑1 (MQ=60)
    CTGATGTTAGCGTCGATGTATTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGG                                                                                                                                                  <  SRR19973080.778983/150‑1 (MQ=60)
           TAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAG                                                                                                                                           <  SRR19973080.691253/150‑1 (MQ=60)
           TAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAG                                                                                                                                           <  SRR19973080.2332267/150‑1 (MQ=60)
             GCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTG                                                                                                                                         <  SRR19973080.104872/150‑1 (MQ=60)
               GTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGT                                                                                                                                       <  SRR19973080.145764/150‑1 (MQ=60)
               GTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGT                                                                                                                                       <  SRR19973080.770960/150‑1 (MQ=60)
                 CGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGC                                                                                                                                     >  SRR19973080.1310889/1‑150 (MQ=60)
                 CGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGC                                                                                                                                     >  SRR19973080.2499407/1‑150 (MQ=60)
                 CGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGC                                                                                                                                     >  SRR19973080.3603935/1‑150 (MQ=60)
                    TGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACT                                                                                                                                  >  SRR19973080.2733716/1‑150 (MQ=60)
                    TGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACT                                                                                                                                  >  SRR19973080.440712/1‑150 (MQ=60)
                     GTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTG                                                                                                                                 >  SRR19973080.3525355/1‑150 (MQ=60)
                     GTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTG                                                                                                                                 <  SRR19973080.1784751/150‑1 (MQ=60)
                       GTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCC                                                                                                                               <  SRR19973080.574800/150‑1 (MQ=60)
                          AGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTT                                                                                                                            >  SRR19973080.4472253/1‑150 (MQ=60)
                          AGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTT                                                                                                                            >  SRR19973080.4470331/1‑150 (MQ=60)
                             CGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATG                                                                                                                         <  SRR19973080.1919844/150‑1 (MQ=60)
                              GGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGG                                                                                                                        <  SRR19973080.2513847/150‑1 (MQ=60)
                              GGTAACGTACTGATGAAACAATTACGTATGCCGCAACAGGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGG                                                                                                                        <  SRR19973080.908628/150‑1 (MQ=60)
                               GTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGC                                                                                                                       <  SRR19973080.2721731/150‑1 (MQ=60)
                                 AACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTG                                                                                                                     <  SRR19973080.715830/150‑1 (MQ=60)
                                    GTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAAC                                                                                                                  <  SRR19973080.2453579/150‑1 (MQ=60)
                                    GTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAAC                                                                                                                  <  SRR19973080.685118/150‑1 (MQ=60)
                                       CTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAAT                                                                                                               >  SRR19973080.1114311/1‑150 (MQ=60)
                                          ATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAA                                                                                                            <  SRR19973080.1481490/150‑1 (MQ=60)
                                           TGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAA                                                                                                           <  SRR19973080.3827281/150‑1 (MQ=60)
                                            GAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAA                                                                                                          <  SRR19973080.3975433/150‑1 (MQ=60)
                                            GAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAA                                                                                                          <  SRR19973080.3235955/150‑1 (MQ=60)
                                            GAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAA                                                                                                          <  SRR19973080.21524/150‑1 (MQ=60)
                                              AACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGACCTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAAT                                                                                                        >  SRR19973080.1104570/1‑150 (MQ=60)
                                              AACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAAT                                                                                                        >  SRR19973080.2063046/1‑150 (MQ=60)
                                              AACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAAG                                                                                                        >  SRR19973080.941965/1‑150 (MQ=60)
                                                 AATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGA                                                                                                     >  SRR19973080.3143526/1‑150 (MQ=60)
                                                 AATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGA                                                                                                     >  SRR19973080.4085016/1‑150 (MQ=60)
                                                  ATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGAT                                                                                                    <  SRR19973080.4174976/150‑1 (MQ=60)
                                                      CGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTG                                                                                                <  SRR19973080.2598713/150‑1 (MQ=60)
                                                       GTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGA                                                                                               >  SRR19973080.1458169/1‑150 (MQ=60)
                                                              GCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGG                                                                                        >  SRR19973080.324800/1‑150 (MQ=60)
                                                               CAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGC                                                                                       <  SRR19973080.3384206/150‑1 (MQ=60)
                                                               CAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGC                                                                                       >  SRR19973080.3333682/1‑150 (MQ=60)
                                                               CAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGC                                                                                       <  SRR19973080.187052/150‑1 (MQ=60)
                                                                  CATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGG                                                                                    <  SRR19973080.1402236/150‑1 (MQ=60)
                                                                  CATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGG                                                                                    <  SRR19973080.1402151/150‑1 (MQ=60)
                                                                  CATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAAATCGCCATGTCCTGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAATATGGCTGG                                                                                    >  SRR19973080.1711625/1‑150 (MQ=60)
                                                                     GACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTG                                                                                 >  SRR19973080.1089274/1‑150 (MQ=60)
                                                                     GACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTG                                                                                 <  SRR19973080.3270659/150‑1 (MQ=60)
                                                                     GACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTG                                                                                 >  SRR19973080.3737642/1‑150 (MQ=60)
                                                                           GCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAAT                                                                           <  SRR19973080.3372857/150‑1 (MQ=60)
                                                                                GTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGG                                                                      >  SRR19973080.3946703/1‑150 (MQ=60)
                                                                                GTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGG                                                                      <  SRR19973080.2572460/150‑1 (MQ=60)
                                                                                GTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGG                                                                      >  SRR19973080.4100682/1‑150 (MQ=60)
                                                                                   GCGGCTGAATAATCTCTCATCCAGCGATCTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCC                                                                   <  SRR19973080.4239367/150‑1 (MQ=60)
                                                                                   GCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCC                                                                   <  SRR19973080.2452423/150‑1 (MQ=60)
                                                                                   GCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCC                                                                   <  SRR19973080.3732101/150‑1 (MQ=60)
                                                                                      GCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGAT                                                                <  SRR19973080.3551505/150‑1 (MQ=60)
                                                                                      GCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGAT                                                                <  SRR19973080.1638619/150‑1 (MQ=60)
                                                                                        TGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGA                                                              >  SRR19973080.3097026/1‑150 (MQ=60)
                                                                                        TGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGA                                                              <  SRR19973080.3179908/150‑1 (MQ=60)
                                                                                        TGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGA                                                              <  SRR19973080.3179974/150‑1 (MQ=60)
                                                                                          AATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACA                                                            >  SRR19973080.2835989/1‑150 (MQ=60)
                                                                                          AATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACA                                                            <  SRR19973080.1171869/150‑1 (MQ=60)
                                                                                            TAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATT                                                          >  SRR19973080.1041402/1‑150 (MQ=60)
                                                                                              CTCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGC                                                        <  SRR19973080.3461812/150‑1 (MQ=60)
                                                                                               TCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCG                                                       <  SRR19973080.816051/150‑1 (MQ=60)
                                                                                                 TCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGAC                                                     >  SRR19973080.2179183/1‑150 (MQ=60)
                                                                                                   TCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTG                                                   >  SRR19973080.3890031/1‑150 (MQ=60)
                                                                                                   TCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTG                                                   >  SRR19973080.3891470/1‑150 (MQ=60)
                                                                                                     ATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGA                                                 <  SRR19973080.3284494/150‑1 (MQ=60)
                                                                                                     ATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGA                                                 >  SRR19973080.1603186/1‑150 (MQ=60)
                                                                                                       CCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATA                                               <  SRR19973080.1091497/150‑1 (MQ=60)
                                                                                                       CCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATA                                               <  SRR19973080.1094081/150‑1 (MQ=60)
                                                                                                       CCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATA                                               <  SRR19973080.2183541/150‑1 (MQ=60)
                                                                                                        CAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAA                                              <  SRR19973080.1429922/150‑1 (MQ=60)
                                                                                                         AGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAA                                             >  SRR19973080.3176977/1‑150 (MQ=60)
                                                                                                         AGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAA                                             >  SRR19973080.3177086/1‑150 (MQ=60)
                                                                                                          GCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAG                                            <  SRR19973080.1586709/150‑1 (MQ=60)
                                                                                                           CGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGA                                           >  SRR19973080.3375801/1‑150 (MQ=60)
                                                                                                             AACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACA                                         >  SRR19973080.4194829/1‑150 (MQ=60)
                                                                                                              ACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACAC                                        >  SRR19973080.2113178/1‑150 (MQ=60)
                                                                                                               CTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACT                                       <  SRR19973080.3352090/150‑1 (MQ=60)
                                                                                                                  GTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCG                                    >  SRR19973080.1533252/1‑150 (MQ=60)
                                                                                                                           GTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTG                           <  SRR19973080.1039604/150‑1 (MQ=60)
                                                                                                                           GTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTG                           >  SRR19973080.1883644/1‑150 (MQ=60)
                                                                                                                           GTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTG                           <  SRR19973080.1039482/150‑1 (MQ=60)
                                                                                                                           GTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTG                           >  SRR19973080.2180974/1‑150 (MQ=60)
                                                                                                                           GTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTG                           <  SRR19973080.2910453/150‑1 (MQ=60)
                                                                                                                            TCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGG                          <  SRR19973080.702701/150‑1 (MQ=60)
                                                                                                                            TCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGG                          <  SRR19973080.2977464/150‑1 (MQ=60)
                                                                                                                             CAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACGGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGT                         <  SRR19973080.2676068/150‑1 (MQ=60)
                                                                                                                              AATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTA                        <  SRR19973080.1481862/150‑1 (MQ=60)
                                                                                                                                TCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAA                      >  SRR19973080.1345196/1‑150 (MQ=60)
                                                                                                                                 CCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAA                     >  SRR19973080.1043415/1‑150 (MQ=60)
                                                                                                                                  CGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAG                    <  SRR19973080.13461/150‑1 (MQ=60)
                                                                                                                                     AACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACA                 >  SRR19973080.2555176/1‑150 (MQ=60)
                                                                                                                                      ACAAGTCGCCAGGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAA                <  SRR19973080.3284139/150‑1 (MQ=60)
                                                                                                                                        AATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATA              >  SRR19973080.1928005/1‑150 (MQ=60)
                                                                                                                                        AATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATA              >  SRR19973080.1132194/1‑150 (MQ=60)
                                                                                                                                        AATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATA              >  SRR19973080.3384408/1‑150 (MQ=60)
                                                                                                                                         ATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATAT             <  SRR19973080.4126955/150‑1 (MQ=60)
                                                                                                                                          TTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATG            <  SRR19973080.1056748/150‑1 (MQ=60)
                                                                                                                                          TTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATG            <  SRR19973080.1056612/150‑1 (MQ=60)
                                                                                                                                          TTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATG            <  SRR19973080.2789617/150‑1 (MQ=60)
                                                                                                                                          TTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATG            >  SRR19973080.2284725/1‑150 (MQ=60)
                                                                                                                                            CGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGAC          <  SRR19973080.176996/150‑1 (MQ=60)
                                                                                                                                              CCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTG        <  SRR19973080.4511783/150‑1 (MQ=60)
                                                                                                                                               CATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGC       >  SRR19973080.1268339/1‑150 (MQ=60)
                                                                                                                                                ATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGCG      >  SRR19973080.2205392/1‑150 (MQ=60)
                                                                                                                                                ATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGCG      >  SRR19973080.3955298/1‑150 (MQ=60)
                                                                                                                                                 TGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGCGG     >  SRR19973080.542573/1‑150 (MQ=60)
                                                                                                                                                  GTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGCGGG    >  SRR19973080.4399113/1‑150 (MQ=60)
                                                                                                                                                  GTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGCGGG    >  SRR19973080.1297320/1‑150 (MQ=60)
                                                                                                                                                    CCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGCGGGTT  <  SRR19973080.503877/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
TTGACTGATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGCGGGTT  >  CP009273/1439369‑1439666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑