Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,439,518 C→A 100% S737S (TCC→TCA)  ydbH → putative membrane‑anchored protein, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,439,5180CA96.7% 864.3 / 16.4 262S737S (TCC→TCA) ydbHputative membrane‑anchored protein, function unknown
Reads supporting (aligned to +/- strand):  ref base C (3/6);  new base A (123/130);  total (126/136)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 263 total aligned reads displayed.

TTGACTGATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGCGGG  >  CP009273/1439369‑1439664
                                                                                                                                                     |                                                                                                                                                  
   aCTGATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAggg                                                                                                                                                 >  1:1277498/1‑150 (MQ=255)
      gATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAAATGGTTAGCGCCGTCAATCCGAAACAAATCGCCAAGTCAGGGGCa                                                                                                                                              >  2:3355776/1‑150 (MQ=255)
       aTGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCAt                                                                                                                                             <  1:861172/150‑1 (MQ=255)
         gTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCAttt                                                                                                                                           <  2:539740/150‑1 (MQ=255)
          ttAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTa                                                                                                                                          >  2:2067177/1‑150 (MQ=255)
          ttAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTa                                                                                                                                          <  2:2319599/150‑1 (MQ=255)
          ttAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTa                                                                                                                                          >  2:3719102/1‑150 (MQ=255)
           tAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAg                                                                                                                                         >  1:3292259/1‑150 (MQ=255)
           tAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAg                                                                                                                                         <  1:266063/150‑1 (MQ=255)
           tAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCAGTTAg                                                                                                                                         >  2:932631/1‑150 (MQ=255)
            aGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGTCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGACGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAgt                                                                                                                                        <  2:3564969/150‑1 (MQ=255)
            aGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAgt                                                                                                                                        <  2:3565110/150‑1 (MQ=255)
            aGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAgt                                                                                                                                        <  1:66898/150‑1 (MQ=255)
                tCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAgtggtg                                                                                                                                    <  2:2850231/150‑1 (MQ=255)
                    tgtgTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACt                                                                                                                                <  2:408721/150‑1 (MQ=255)
                    tgtgTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACt                                                                                                                                >  2:1951561/1‑150 (MQ=255)
                      tgtTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGc                                                                                                                              >  2:3903037/1‑150 (MQ=255)
                      tgtTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGc                                                                                                                              <  2:1665982/150‑1 (MQ=255)
                       gtTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGcc                                                                                                                             <  1:1785319/150‑1 (MQ=255)
                        ttAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCg                                                                                                                            >  2:686212/1‑150 (MQ=255)
                          aGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGtt                                                                                                                          >  1:719792/1‑150 (MQ=255)
                          aGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGtt                                                                                                                          <  2:2515817/150‑1 (MQ=255)
                           ggCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTa                                                                                                                         <  2:713284/150‑1 (MQ=255)
                               gTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGc                                                                                                                     <  2:3131003/150‑1 (MQ=255)
                                tAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCt                                                                                                                    <  1:4260068/150‑1 (MQ=255)
                                tAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCt                                                                                                                    <  2:3969734/150‑1 (MQ=255)
                                 aaCGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTg                                                                                                                   <  2:4396977/150‑1 (MQ=255)
                                  aCGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGa                                                                                                                  <  2:2368087/150‑1 (MQ=255)
                                  aCGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGa                                                                                                                  <  2:22531/150‑1 (MQ=255)
                                   cGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGaa                                                                                                                 <  2:4423933/150‑1 (MQ=255)
                                   cGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGaa                                                                                                                 >  2:1413880/1‑150 (MQ=255)
                                   cGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGaa                                                                                                                 >  2:3701074/1‑150 (MQ=255)
                                    gTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAAc                                                                                                                <  1:4228742/150‑1 (MQ=255)
                                    gTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAAc                                                                                                                >  1:1026605/1‑150 (MQ=255)
                                     tACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACa                                                                                                               <  1:2015285/150‑1 (MQ=255)
                                       cTGATGAAACAATTACGTATGCCGAAACATGACCAGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGACATGTCCGGTGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAAt                                                                                                             <  1:3203427/150‑1 (MQ=255)
                                           tgaAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGaaa                                                                                                         <  1:1833101/150‑1 (MQ=255)
                                            gaAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGaaaa                                                                                                        >  1:1454063/1‑150 (MQ=255)
                                                cAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTTGTTAGCGACGTCAATCAGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATgg                                                                                                    <  1:1077628/150‑1 (MQ=255)
                                                cAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATgg                                                                                                    <  1:4631392/150‑1 (MQ=255)
                                                  aTTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGAt                                                                                                  <  1:1414184/150‑1 (MQ=255)
                                                    tACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATtg                                                                                                <  1:1195116/150‑1 (MQ=255)
                                                     aCGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATtgt                                                                                               <  1:4366325/150‑1 (MQ=255)
                                                       gTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGa                                                                                             <  2:3906472/150‑1 (MQ=255)
                                                         aTGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGaaa                                                                                           >  1:416586/1‑150 (MQ=255)
                                                         aTGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGaaa                                                                                           >  2:177037/1‑150 (MQ=255)
                                                           gCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGa                                                                                         >  1:4622772/1‑150 (MQ=255)
                                                            ccGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGAt                                                                                        <  1:40433/150‑1 (MQ=255)
                                                             cGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGAtg                                                                                       <  2:1572439/150‑1 (MQ=255)
                                                             cGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGAtg                                                                                       >  2:2093733/1‑150 (MQ=255)
                                                                 aCATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGAtggctg                                                                                   <  1:3764330/150‑1 (MQ=255)
                                                                 aCATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGAtggctg                                                                                   >  1:4492840/1‑150 (MQ=255)
                                                                  cATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGAtggctgg                                                                                  >  1:1702187/1‑150 (MQ=255)
                                                                   aTGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGAtggctggc                                                                                 >  2:1550764/1‑150 (MQ=255)
                                                                          ggCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGaa                                                                          >  1:4524097/1‑150 (MQ=255)
                                                                           gcgcTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAAt                                                                         <  1:116087/150‑1 (MQ=255)
                                                                               tGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCg                                                                     >  2:4014148/1‑150 (MQ=255)
                                                                               tGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCg                                                                     >  2:2018260/1‑150 (MQ=255)
                                                                               tGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCg                                                                     >  2:4558554/1‑150 (MQ=255)
                                                                                  tGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGc                                                                  >  1:2735055/1‑150 (MQ=255)
                                                                                     ggCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGa                                                               >  2:3248489/1‑150 (MQ=255)
                                                                                     ggCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGa                                                               <  2:625700/150‑1 (MQ=255)
                                                                                      gCTGAATAATCACTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAAAAATTCTCCAAGTCAGGGGCATGTAGTTGTGCACTGCCGTTATGTCTGAACAATGAAAAAAGGATAGTGAAAGATGGCTGGCTGTCGAATAGCGGGCCGAt                                                              >  2:2452140/1‑150 (MQ=255)
                                                                                       cTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATg                                                             <  1:3101554/150‑1 (MQ=255)
                                                                                          aataatCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACa                                                          <  1:3749771/150‑1 (MQ=255)
                                                                                           ataatCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACAt                                                         <  1:3741187/150‑1 (MQ=255)
                                                                                           ataatCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACAt                                                         <  1:2934045/150‑1 (MQ=255)
                                                                                            taatCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACAtt                                                        >  1:249250/1‑150 (MQ=255)
                                                                                            taatCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACAtt                                                        <  1:2523831/150‑1 (MQ=255)
                                                                                            taatCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACAtt                                                        <  1:2876271/150‑1 (MQ=255)
                                                                                              atCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATATAGAGGTGCACTGCCGTTATGGCTTAACAATGAAAAATTGATTTTGAAAGATGGCTGGCTGGCGAATATCGGGCCGATTACATTGc                                                      >  1:395137/1‑150 (MQ=255)
                                                                                              atCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGc                                                      >  2:4001900/1‑150 (MQ=255)
                                                                                                 tctcATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGAc                                                   >  1:780668/1‑150 (MQ=255)
                                                                                                     aTCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGa                                               <  1:3247623/150‑1 (MQ=255)
                                                                                                     aTCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGa                                               >  1:3520388/1‑150 (MQ=255)
                                                                                                      tCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGAt                                              <  2:3129554/150‑1 (MQ=255)
                                                                                                           cGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGa                                         >  1:899408/1‑150 (MQ=255)
                                                                                                                tGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTg                                    >  2:1946347/1‑150 (MQ=255)
                                                                                                                   ttAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCgg                                 >  1:3404137/1‑150 (MQ=255)
                                                                                                                          cGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCggt                          <  2:1187819/150‑1 (MQ=255)
                                                                                                                           gTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCggtg                         >  2:2808597/1‑150 (MQ=255)
                                                                                                                           gTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCggtg                         <  2:1117735/150‑1 (MQ=255)
                                                                                                                            tCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCggtgg                        >  1:622305/1‑150 (MQ=255)
                                                                                                                            tCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCggtgg                        >  1:2069928/1‑150 (MQ=255)
                                                                                                                            tCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCggtgg                        >  2:344628/1‑150 (MQ=255)
                                                                                                                              aaTCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTa                      >  1:1348582/1‑150 (MQ=255)
                                                                                                                               aTCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTaa                     >  2:4278781/1‑150 (MQ=255)
                                                                                                                               aTCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTaa                     <  1:493450/150‑1 (MQ=255)
                                                                                                                               aTCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTaa                     >  1:2192177/1‑150 (MQ=255)
                                                                                                                               aTCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTaa                     <  1:2804893/150‑1 (MQ=255)
                                                                                                                                tCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTaaa                    >  2:3124931/1‑150 (MQ=255)
                                                                                                                                 ccGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTaaaa                   >  1:53803/1‑150 (MQ=255)
                                                                                                                                  cGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAg                  >  1:1850380/1‑150 (MQ=255)
                                                                                                                                           tCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGa         >  2:1506710/1‑150 (MQ=255)
                                                                                                                                            cGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGAc        >  2:2178832/1‑150 (MQ=255)
                                                                                                                                              ccATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTg      >  2:4309992/1‑150 (MQ=255)
                                                                                                                                               cATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGc     <  2:2593885/150‑1 (MQ=255)
                                                                                                                                                aTGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGCg    >  1:2286753/1‑150 (MQ=255)
                                                                                                                                                  gTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGATAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGCggg  <  2:735731/150‑1 (MQ=255)
                                                                                                                                                  gTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGCggg  >  2:331107/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
TTGACTGATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGCGGG  >  CP009273/1439369‑1439664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTGACTGATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGCGGGTTC  >  CP009273/1439369‑1439667
                                                                                                                                                     |                                                                                                                                                     
TTGACTGATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCC                                                                                                                                                       >  SRR19973073.2837531/1‑150 (MQ=60)
TTGACTGATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCA                                                                                                                                                       >  SRR19973073.1914976/1‑150 (MQ=60)
 TGACTGATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAG                                                                                                                                                      >  SRR19973073.1095883/1‑150 (MQ=60)
 TGACTGATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAG                                                                                                                                                      >  SRR19973073.2088943/1‑150 (MQ=60)
   ACTGATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGG                                                                                                                                                    >  SRR19973073.1280182/1‑150 (MQ=60)
   ACTGATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGG                                                                                                                                                    <  SRR19973073.2962334/150‑1 (MQ=60)
      GATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCA                                                                                                                                                 >  SRR19973073.691817/1‑150 (MQ=60)
       ATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCAT                                                                                                                                                <  SRR19973073.863021/150‑1 (MQ=60)
       ATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCAT                                                                                                                                                <  SRR19973073.862956/150‑1 (MQ=60)
           TAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAG                                                                                                                                            >  SRR19973073.3298933/1‑150 (MQ=60)
           TAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAG                                                                                                                                            <  SRR19973073.266648/150‑1 (MQ=60)
           TAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAG                                                                                                                                            >  SRR19973073.3298927/1‑150 (MQ=60)
            AGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGT                                                                                                                                           <  SRR19973073.67084/150‑1 (MQ=60)
            AGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGT                                                                                                                                           <  SRR19973073.2181791/150‑1 (MQ=60)
               GTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGT                                                                                                                                        >  SRR19973073.4269638/1‑150 (MQ=60)
               GTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGT                                                                                                                                        >  SRR19973073.4372239/1‑150 (MQ=60)
                TCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAGACAATTCGCCATGTCAGGGGCATTTAGTGGTG                                                                                                                                       <  SRR19973073.3145396/150‑1 (MQ=60)
                 CGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGC                                                                                                                                      <  SRR19973073.2828343/150‑1 (MQ=60)
                 CGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGC                                                                                                                                      <  SRR19973073.2036732/150‑1 (MQ=60)
                 CGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGC                                                                                                                                      >  SRR19973073.1733708/1‑150 (MQ=60)
                   ATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCAC                                                                                                                                    <  SRR19973073.1028884/150‑1 (MQ=60)
                    TGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACT                                                                                                                                   <  SRR19973073.2065105/150‑1 (MQ=60)
                       GTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCC                                                                                                                                <  SRR19973073.1789009/150‑1 (MQ=60)
                       GTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCC                                                                                                                                <  SRR19973073.131199/150‑1 (MQ=60)
                         TAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGT                                                                                                                              >  SRR19973073.620082/1‑150 (MQ=60)
                          AGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTT                                                                                                                             >  SRR19973073.721273/1‑150 (MQ=60)
                          AGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTAATCCAGCGAACTGGTTAGCGCCTTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTT                                                                                                                             >  SRR19973073.280478/1‑150 (MQ=60)
                            GCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTAT                                                                                                                           <  SRR19973073.853936/150‑1 (MQ=60)
                                TAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCT                                                                                                                       <  SRR19973073.4268505/150‑1 (MQ=60)
                                 AACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTG                                                                                                                      >  SRR19973073.414630/1‑150 (MQ=60)
                                   CGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAA                                                                                                                    >  SRR19973073.3266362/1‑150 (MQ=60)
                                    GTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAAC                                                                                                                   >  SRR19973073.1028748/1‑150 (MQ=60)
                                    GTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAAC                                                                                                                   <  SRR19973073.1341106/150‑1 (MQ=60)
                                    GTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAAC                                                                                                                   <  SRR19973073.2388239/150‑1 (MQ=60)
                                    GTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAAC                                                                                                                   <  SRR19973073.4237122/150‑1 (MQ=60)
                                     TACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACA                                                                                                                  <  SRR19973073.1506262/150‑1 (MQ=60)
                                     TACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACA                                                                                                                  <  SRR19973073.2019479/150‑1 (MQ=60)
                                      ACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAA                                                                                                                 >  SRR19973073.73729/1‑150 (MQ=60)
                                      ACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAA                                                                                                                 >  SRR19973073.73662/1‑150 (MQ=60)
                                       CTGATGAAACAATTACGTATGCCGAAACATGACCAGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGACATGTCCGGTGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAAT                                                                                                                <  SRR19973073.3209901/150‑1 (MQ=60)
                                          ATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAA                                                                                                             <  SRR19973073.4536295/150‑1 (MQ=60)
                                          ATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAA                                                                                                             <  SRR19973073.4536185/150‑1 (MQ=60)
                                           TGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAA                                                                                                            <  SRR19973073.1836880/150‑1 (MQ=60)
                                            GAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAA                                                                                                           >  SRR19973073.1457117/1‑150 (MQ=60)
                                             AAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAA                                                                                                          >  SRR19973073.2759289/1‑150 (MQ=60)
                                              AACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAAT                                                                                                         >  SRR19973073.4189894/1‑150 (MQ=60)
                                              AACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAAT                                                                                                         >  SRR19973073.4189957/1‑150 (MQ=60)
                                                CAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTTGTTAGCGACGTCAATCAGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGG                                                                                                       <  SRR19973073.1079851/150‑1 (MQ=60)
                                                CAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGG                                                                                                       <  SRR19973073.2480739/150‑1 (MQ=60)
                                                CAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGG                                                                                                       <  SRR19973073.3608726/150‑1 (MQ=60)
                                                CAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGG                                                                                                       <  SRR19973073.4640625/150‑1 (MQ=60)
                                                CAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACGGCCGTTATGGCTGAACAATGAAAAATGG                                                                                                       <  SRR19973073.4640600/150‑1 (MQ=60)
                                                  ATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGAT                                                                                                     >  SRR19973073.4154756/1‑150 (MQ=60)
                                                  ATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGAT                                                                                                     <  SRR19973073.1417167/150‑1 (MQ=60)
                                                    TACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTG                                                                                                   <  SRR19973073.1197593/150‑1 (MQ=60)
                                                     ACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGT                                                                                                  <  SRR19973073.4375074/150‑1 (MQ=60)
                                                       GTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGA                                                                                                >  SRR19973073.1452522/1‑150 (MQ=60)
                                                        TATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAA                                                                                               >  SRR19973073.3483937/1‑150 (MQ=60)
                                                        TATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAAAAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAA                                                                                               <  SRR19973073.631234/150‑1 (MQ=60)
                                                         ATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAA                                                                                              >  SRR19973073.417417/1‑150 (MQ=60)
                                                         ATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAA                                                                                              >  SRR19973073.4515785/1‑150 (MQ=60)
                                                           GCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGA                                                                                            >  SRR19973073.4631995/1‑150 (MQ=60)
                                                            CCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGAT                                                                                           <  SRR19973073.40555/150‑1 (MQ=60)
                                                              GCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGTCTGAACAATGAAAAATGGATTGTGAAAGATGT                                                                                         >  SRR19973073.1275076/1‑150 (MQ=60)
                                                               CAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGC                                                                                        <  SRR19973073.1462497/150‑1 (MQ=60)
                                                               CAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGC                                                                                        <  SRR19973073.1435200/150‑1 (MQ=60)
                                                                 ACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTG                                                                                      >  SRR19973073.4501872/1‑150 (MQ=60)
                                                                 ACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTG                                                                                      <  SRR19973073.3771873/150‑1 (MQ=60)
                                                                  CATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGG                                                                                     >  SRR19973073.1705720/1‑150 (MQ=60)
                                                                      ACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGG                                                                                 >  SRR19973073.3218125/1‑150 (MQ=60)
                                                                          GGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAA                                                                             >  SRR19973073.4533173/1‑150 (MQ=60)
                                                                          GGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAA                                                                             >  SRR19973073.4533018/1‑150 (MQ=60)
                                                                           GCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAAT                                                                            <  SRR19973073.116320/150‑1 (MQ=60)
                                                                           GCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAAT                                                                            <  SRR19973073.116372/150‑1 (MQ=60)
                                                                               TGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCG                                                                        <  SRR19973073.4258995/150‑1 (MQ=60)
                                                                               TGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCG                                                                        <  SRR19973073.493347/150‑1 (MQ=60)
                                                                                  TGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGC                                                                     >  SRR19973073.2740641/1‑150 (MQ=60)
                                                                                       CTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATG                                                                <  SRR19973073.3107794/150‑1 (MQ=60)
                                                                                          AATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACA                                                             <  SRR19973073.3757248/150‑1 (MQ=60)
                                                                                          AATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACA                                                             <  SRR19973073.3757293/150‑1 (MQ=60)
                                                                                           ATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACAT                                                            <  SRR19973073.3748696/150‑1 (MQ=60)
                                                                                           ATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACAT                                                            <  SRR19973073.328742/150‑1 (MQ=60)
                                                                                           ATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACAT                                                            <  SRR19973073.3113650/150‑1 (MQ=60)
                                                                                           ATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACAT                                                            <  SRR19973073.2940043/150‑1 (MQ=60)
                                                                                            TAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATT                                                           <  SRR19973073.2529043/150‑1 (MQ=60)
                                                                                            TAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATT                                                           >  SRR19973073.249814/1‑150 (MQ=60)
                                                                                            TAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATT                                                           >  SRR19973073.250000/1‑150 (MQ=60)
                                                                                            TAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATT                                                           <  SRR19973073.2882149/150‑1 (MQ=60)
                                                                                              ATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATATAGAGGTGCACTGCCGTTATGGCTTAACAATGAAAAATTGATTTTGAAAGATGGCTGGCTGGCGAATATCGGGCCGATTACATTGC                                                         >  SRR19973073.395924/1‑150 (MQ=60)
                                                                                               TCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGTTGGCGAATAGCGGGCCGATGACATTGCG                                                        <  SRR19973073.2269528/150‑1 (MQ=60)
                                                                                                CTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGA                                                       >  SRR19973073.2674739/1‑150 (MQ=60)
                                                                                                 TCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGAC                                                      >  SRR19973073.782247/1‑150 (MQ=60)
                                                                                                 TCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGAC                                                      >  SRR19973073.3103811/1‑150 (MQ=60)
                                                                                                   TCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTGAGGTGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAATATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTG                                                    <  SRR19973073.1804311/150‑1 (MQ=60)
                                                                                                    CATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGG                                                   >  SRR19973073.3301909/1‑150 (MQ=60)
                                                                                                     ATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGA                                                  <  SRR19973073.3254184/150‑1 (MQ=60)
                                                                                                     ATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGA                                                  >  SRR19973073.3527426/1‑150 (MQ=60)
                                                                                                        CAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAA                                               >  SRR19973073.1181298/1‑150 (MQ=60)
                                                                                                          GCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAG                                             <  SRR19973073.3611570/150‑1 (MQ=60)
                                                                                                           CGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGA                                            >  SRR19973073.3835169/1‑150 (MQ=60)
                                                                                                           CGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGA                                            >  SRR19973073.901265/1‑150 (MQ=60)
                                                                                                           CGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGA                                            >  SRR19973073.3835165/1‑150 (MQ=60)
                                                                                                             AACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACA                                          <  SRR19973073.2885341/150‑1 (MQ=60)
                                                                                                                  GTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGACTAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCG                                     >  SRR19973073.4645657/1‑150 (MQ=60)
                                                                                                                  GTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCG                                     <  SRR19973073.806025/150‑1 (MQ=60)
                                                                                                                   TTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGG                                    >  SRR19973073.1088004/1‑150 (MQ=60)
                                                                                                                   TTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGG                                    >  SRR19973073.3410959/1‑150 (MQ=60)
                                                                                                                     AGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGAT                                  >  SRR19973073.4386040/1‑150 (MQ=60)
                                                                                                                            TCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGG                           >  SRR19973073.2074247/1‑150 (MQ=60)
                                                                                                                            TCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGG                           >  SRR19973073.623635/1‑150 (MQ=60)
                                                                                                                              AATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTA                         >  SRR19973073.1351446/1‑150 (MQ=60)
                                                                                                                              AATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTA                         >  SRR19973073.1351594/1‑150 (MQ=60)
                                                                                                                               ATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAA                        >  SRR19973073.2196748/1‑150 (MQ=60)
                                                                                                                               ATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAA                        <  SRR19973073.2810643/150‑1 (MQ=60)
                                                                                                                               ATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAA                        <  SRR19973073.494574/150‑1 (MQ=60)
                                                                                                                               ATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAA                        <  SRR19973073.494501/150‑1 (MQ=60)
                                                                                                                                TCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAA                       <  SRR19973073.182404/150‑1 (MQ=60)
                                                                                                                                TCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAA                       <  SRR19973073.1961752/150‑1 (MQ=60)
                                                                                                                                 CCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAA                      >  SRR19973073.53961/1‑150 (MQ=60)
                                                                                                                                 CCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAA                      >  SRR19973073.53820/1‑150 (MQ=60)
                                                                                                                                  CGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAG                     >  SRR19973073.1854061/1‑150 (MQ=60)
                                                                                                                                  CGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAG                     >  SRR19973073.1854182/1‑150 (MQ=60)
                                                                                                                                       CAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAAT                >  SRR19973073.1977948/1‑150 (MQ=60)
                                                                                                                                        AATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATA               <  SRR19973073.3764398/150‑1 (MQ=60)
                                                                                                                                        AATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATA               <  SRR19973073.3764461/150‑1 (MQ=60)
                                                                                                                                         ATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATAT              <  SRR19973073.1263269/150‑1 (MQ=60)
                                                                                                                                            CGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGAC           <  SRR19973073.4029060/150‑1 (MQ=60)
                                                                                                                                            CGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGAC           <  SRR19973073.2556435/150‑1 (MQ=60)
                                                                                                                                             GCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACT          <  SRR19973073.2588138/150‑1 (MQ=60)
                                                                                                                                              CCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTG         >  SRR19973073.632856/1‑150 (MQ=60)
                                                                                                                                                ATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGCG       >  SRR19973073.2291365/1‑150 (MQ=60)
                                                                                                                                                ATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGCG       >  SRR19973073.2291421/1‑150 (MQ=60)
                                                                                                                                                     AGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGTTTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACTCTGCGGATGCGGTGGTAAAAGACAATATGACTGCGGGTTC  <  SRR19973073.107610/150‑1 (MQ=60)
                                                                                                                                                     AGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGCGGGTTC  <  SRR19973073.4115649/150‑1 (MQ=60)
                                                                                                                                                     AGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGCGGGTTC  >  SRR19973073.19614/1‑150 (MQ=60)
                                                                                                                                                     AGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGCGGGTTC  <  SRR19973073.2996770/150‑1 (MQ=60)
                                                                                                                                                     AGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGCGGGTTC  <  SRR19973073.3931796/150‑1 (MQ=60)
                                                                                                                                                     AGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGCGGGTTC  <  SRR19973073.2525582/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
TTGACTGATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGCGGGTTC  >  CP009273/1439369‑1439667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑