Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,439,518 C→A 100% S737S (TCC→TCA)  ydbH → putative membrane‑anchored protein, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,439,5180CA98.6% 710.6 / 0.4 220S737S (TCC→TCA) ydbHputative membrane‑anchored protein, function unknown
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base A (100/117);  total (101/119)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 220 total aligned reads displayed.

CTGATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGCG  >  CP009273/1439373‑1439662
                                                                                                                                                 |                                                                                                                                                
 tGATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAggg                                                                                                                                               >  2:571859/1‑148 (MQ=255)
 tGATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTAGCCATGTCAggg                                                                                                                                               >  1:3084710/1‑148 (MQ=255)
  gATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAgggg                                                                                                                                              >  1:2505170/1‑148 (MQ=255)
   aTGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGc                                                                                                                                             >  1:1567836/1‑148 (MQ=255)
    tGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCa                                                                                                                                            <  1:1982045/148‑1 (MQ=255)
     gTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCAt                                                                                                                                           <  1:785320/148‑1 (MQ=255)
      ttAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCAtt                                                                                                                                          <  1:1187386/148‑1 (MQ=255)
       tAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCAttt                                                                                                                                         >  2:848963/1‑148 (MQ=255)
       tAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCAttt                                                                                                                                         >  1:1865693/1‑148 (MQ=255)
         gCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCTCCATGTCAGGGGCATTTAg                                                                                                                                       <  2:1516099/148‑1 (MQ=255)
         gCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCTCCATGTCAGGGGCATTTAg                                                                                                                                       <  2:3278415/148‑1 (MQ=255)
            tCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAgtgg                                                                                                                                    >  1:2930511/1‑148 (MQ=255)
                tgtgTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCa                                                                                                                                >  2:1087015/1‑148 (MQ=255)
                tgtgTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCa                                                                                                                                >  2:3485331/1‑148 (MQ=255)
                 gtgtTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCAc                                                                                                                               >  1:1026423/1‑148 (MQ=255)
                  tgtTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACt                                                                                                                              >  2:2246466/1‑148 (MQ=255)
                   gtTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTg                                                                                                                             <  1:1574862/148‑1 (MQ=255)
                      aGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCg                                                                                                                          <  1:1846692/148‑1 (MQ=255)
                       ggCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGt                                                                                                                         <  1:405040/148‑1 (MQ=255)
                       ggCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGt                                                                                                                         <  1:3498987/148‑1 (MQ=255)
                       ggCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCAGTTAGTGGTGCACTGCCGt                                                                                                                         >  2:2793543/1‑148 (MQ=255)
                        gCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGtt                                                                                                                        <  2:2304241/148‑1 (MQ=255)
                        gCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGtt                                                                                                                        >  1:2649237/1‑148 (MQ=255)
                        gCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGtt                                                                                                                        >  1:2649309/1‑148 (MQ=255)
                            tAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATgg                                                                                                                    <  2:1998958/148‑1 (MQ=255)
                                gTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGa                                                                                                                <  2:1615619/148‑1 (MQ=255)
                                 tACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGaa                                                                                                               >  1:849760/1‑148 (MQ=255)
                                  aCTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAAc                                                                                                              >  2:1579958/1‑148 (MQ=255)
                                  aCTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAAc                                                                                                              >  2:1579932/1‑148 (MQ=255)
                                  aCTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAAc                                                                                                              >  1:1631246/1‑148 (MQ=255)
                                   cTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACa                                                                                                             <  2:3291493/148‑1 (MQ=255)
                                          aaCAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGaaaa                                                                                                      >  2:769508/1‑148 (MQ=255)
                                          aaCAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGaaaa                                                                                                      <  2:731266/148‑1 (MQ=255)
                                          aaCAATTACGTATGCCGCAAAATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGaaaa                                                                                                      <  1:959934/148‑1 (MQ=255)
                                            cAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAAt                                                                                                    <  2:26950/148‑1 (MQ=255)
                                             aaTTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATg                                                                                                   <  2:1311872/148‑1 (MQ=255)
                                              aTTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATgg                                                                                                  <  2:1780274/148‑1 (MQ=255)
                                              aTTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATgg                                                                                                  >  1:468509/1‑148 (MQ=255)
                                               ttACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGa                                                                                                 >  1:1910442/1‑148 (MQ=255)
                                                tACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGAt                                                                                                <  1:2278322/148‑1 (MQ=255)
                                                 aCGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGAtt                                                                                               >  2:1333223/1‑148 (MQ=255)
                                                 aCGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGAtt                                                                                               <  1:656742/148‑1 (MQ=255)
                                                 aCGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGAtt                                                                                               >  2:2650173/1‑148 (MQ=255)
                                                    tATGCCGCAACATGACCCGGCGCTGTTTCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATtgtg                                                                                            >  1:3183970/1‑148 (MQ=255)
                                                       gCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGaaa                                                                                         <  1:2420550/148‑1 (MQ=255)
                                                         cGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGa                                                                                       >  1:2047635/1‑148 (MQ=255)
                                                          gCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGAt                                                                                      >  2:1416420/1‑148 (MQ=255)
                                                           cAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTGAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGAtg                                                                                     <  1:1555357/148‑1 (MQ=255)
                                                           cAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGAtg                                                                                     <  2:493003/148‑1 (MQ=255)
                                                           cAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGAtg                                                                                     >  1:2560450/1‑148 (MQ=255)
                                                            aaCATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGAtgg                                                                                    <  1:2921569/148‑1 (MQ=255)
                                                                tGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGAtggctgg                                                                                <  1:3202371/148‑1 (MQ=255)
                                                                 gACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGAtggctggc                                                                               <  1:1493415/148‑1 (MQ=255)
                                                                     cGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGAtggctggctggc                                                                           >  2:190246/1‑148 (MQ=255)
                                                                       gcgcTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGa                                                                         <  2:1161751/148‑1 (MQ=255)
                                                                        cgcTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGaa                                                                        >  2:2466540/1‑148 (MQ=255)
                                                                         gcTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAAt                                                                       >  2:554437/1‑148 (MQ=255)
                                                                           tGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAACAATGGATTGTGAAAGATGGCTGGCTGGCGAATAg                                                                     <  2:2643410/148‑1 (MQ=255)
                                                                            gTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGc                                                                    <  1:2084615/148‑1 (MQ=255)
                                                                             ttGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCTCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCg                                                                   >  1:2738882/1‑148 (MQ=255)
                                                                             ttGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCg                                                                   <  1:848963/148‑1 (MQ=255)
                                                                             ttGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCg                                                                   <  1:3011040/148‑1 (MQ=255)
                                                                                cGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAAt                                                                       <  2:2853810/141‑1 (MQ=255)
                                                                                cGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAAt                                                                       >  1:1079009/1‑141 (MQ=255)
                                                                                cGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGc                                                                <  1:3547192/148‑1 (MQ=255)
                                                                                cGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGCCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAAt                                                                       <  2:1078996/141‑1 (MQ=255)
                                                                                 ggCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGcc                                                               <  1:1539361/148‑1 (MQ=255)
                                                                                   cTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGa                                                             <  1:3326758/148‑1 (MQ=255)
                                                                                    tGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGAt                                                            <  2:1323363/148‑1 (MQ=255)
                                                                                     gAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATg                                                           >  2:3452357/1‑148 (MQ=255)
                                                                                      aataatCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGa                                                          <  1:438278/148‑1 (MQ=255)
                                                                                      aataatCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGa                                                          >  1:2248443/1‑148 (MQ=255)
                                                                                        taatCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACa                                                        <  2:2570314/148‑1 (MQ=255)
                                                                                         aatCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACAt                                                       <  2:1865693/148‑1 (MQ=255)
                                                                                             tctcATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCg                                                   >  2:3104850/1‑148 (MQ=255)
                                                                                              ctcATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGa                                                  <  1:1991551/148‑1 (MQ=255)
                                                                                                       cGAACTGGTTAGCGGCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATTACATTGCGACTGGATaaa                                         <  2:1053746/148‑1 (MQ=255)
                                                                                                       cGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGATGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATaaa                                         <  2:1053793/148‑1 (MQ=255)
                                                                                                          aCTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGac                                      <  2:2315750/148‑1 (MQ=255)
                                                                                                          aCTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGac                                      <  2:3358403/148‑1 (MQ=255)
                                                                                                            tGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGacac                                    >  2:938172/1‑148 (MQ=255)
                                                                                                                     ccGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGc                           >  2:765504/1‑148 (MQ=255)
                                                                                                                        tCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCggt                        <  2:2949801/148‑1 (MQ=255)
                                                                                                                           aTCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCggtggt                     >  1:1489298/1‑148 (MQ=255)
                                                                                                                             ccGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTaa                   <  2:3183970/148‑1 (MQ=255)
                                                                                                                             ccGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTaa                   >  1:2441322/1‑148 (MQ=255)
                                                                                                                                 aaCAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGa               <  2:375464/148‑1 (MQ=255)
                                                                                                                                     aTTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAat           <  2:1567591/148‑1 (MQ=255)
                                                                                                                                     aTTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAat           >  2:582615/1‑148 (MQ=255)
                                                                                                                                     aTTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAat           <  1:574810/148‑1 (MQ=255)
                                                                                                                                     aTTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAat           >  1:296295/1‑148 (MQ=255)
                                                                                                                                       tCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAatat         <  1:2228889/148‑1 (MQ=255)
                                                                                                                                       tCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAatat         <  1:3410512/148‑1 (MQ=255)
                                                                                                                                           cATGTCAGGGGCATTTAGTGGTGCCCTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATCGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACCATATGACt     >  1:526638/1‑148 (MQ=255)
                                                                                                                                           cATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACt     <  1:195271/148‑1 (MQ=255)
                                                                                                                                            aTGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTg    <  2:1654640/148‑1 (MQ=255)
                                                                                                                                            aTGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTg    <  1:1652546/148‑1 (MQ=255)
                                                                                                                                            aTGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTg    >  1:1579431/1‑148 (MQ=255)
                                                                                                                                            aTGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTg    <  2:1654347/148‑1 (MQ=255)
                                                                                                                                            aTGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTg    >  2:3192553/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CTGATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGCG  >  CP009273/1439373‑1439662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GACTGATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGCGGGTTC  >  CP009273/1439371‑1439667
                                                                                                                                                   |                                                                                                                                                     
GACTGATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGG                                                                                                                                                     <  SRR19973067.2783069/150‑1 (MQ=60)
 ACTGATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTAGCCATGTCAGGG                                                                                                                                                    >  SRR19973067.3104339/1‑150 (MQ=60)
  CTGATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGG                                                                                                                                                   >  SRR19973067.2521609/1‑150 (MQ=60)
  CTGATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGG                                                                                                                                                   >  SRR19973067.2521206/1‑150 (MQ=60)
   TGATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGC                                                                                                                                                  >  SRR19973067.1578558/1‑150 (MQ=60)
      TGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATT                                                                                                                                               <  SRR19973067.3317857/150‑1 (MQ=60)
      TGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATT                                                                                                                                               <  SRR19973067.1995458/150‑1 (MQ=60)
       GTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTT                                                                                                                                              >  SRR19973067.1878422/1‑150 (MQ=60)
       GTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTT                                                                                                                                              <  SRR19973067.876387/150‑1 (MQ=60)
       GTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTT                                                                                                                                              <  SRR19973067.790752/150‑1 (MQ=60)
        TTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTA                                                                                                                                             <  SRR19973067.1195205/150‑1 (MQ=60)
            CGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGG                                                                                                                                         >  SRR19973067.2949124/1‑150 (MQ=60)
                 ATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCAC                                                                                                                                    >  SRR19973067.1033483/1‑150 (MQ=60)
                 ATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCAC                                                                                                                                    >  SRR19973067.490480/1‑150 (MQ=60)
                  TGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACT                                                                                                                                   <  SRR19973067.3128623/150‑1 (MQ=60)
                     GTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCC                                                                                                                                <  SRR19973067.2283983/150‑1 (MQ=60)
                     GTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCC                                                                                                                                <  SRR19973067.1585643/150‑1 (MQ=60)
                        AGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTT                                                                                                                             <  SRR19973067.2016201/150‑1 (MQ=60)
                        AGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTT                                                                                                                             >  SRR19973067.2666326/1‑150 (MQ=60)
                        AGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTT                                                                                                                             >  SRR19973067.2666339/1‑150 (MQ=60)
                        AGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTT                                                                                                                             <  SRR19973067.1894676/150‑1 (MQ=60)
                        AGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTT                                                                                                                             >  SRR19973067.2666398/1‑150 (MQ=60)
                        AGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTT                                                                                                                             <  SRR19973067.1859282/150‑1 (MQ=60)
                         GGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTA                                                                                                                            <  SRR19973067.408142/150‑1 (MQ=60)
                         GGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTA                                                                                                                            <  SRR19973067.407856/150‑1 (MQ=60)
                         GGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTA                                                                                                                            <  SRR19973067.1937503/150‑1 (MQ=60)
                         GGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTA                                                                                                                            <  SRR19973067.3521175/150‑1 (MQ=60)
                           CGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCCTTTAGTGGTGCACTGCCGTTATG                                                                                                                          >  SRR19973067.1529949/1‑150 (MQ=60)
                           CGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATG                                                                                                                          >  SRR19973067.1531377/1‑150 (MQ=60)
                               AACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTG                                                                                                                      <  SRR19973067.2731635/150‑1 (MQ=60)
                                 CGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAA                                                                                                                    >  SRR19973067.855508/1‑150 (MQ=60)
                                  GTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAAC                                                                                                                   >  SRR19973067.1935638/1‑150 (MQ=60)
                                  GTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAAC                                                                                                                   >  SRR19973067.1578313/1‑150 (MQ=60)
                                  GTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAAC                                                                                                                   >  SRR19973067.1642473/1‑150 (MQ=60)
                                   TACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACA                                                                                                                  >  SRR19973067.2957017/1‑150 (MQ=60)
                                   TACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACA                                                                                                                  >  SRR19973067.1169481/1‑150 (MQ=60)
                                   TACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATGTAGTGGTGCACTGCCGTTATGGCTGAACA                                                                                                                  >  SRR19973067.3214719/1‑150 (MQ=60)
                                       GATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGA                                                                                                              <  SRR19973067.650039/150‑1 (MQ=60)
                                         TGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAA                                                                                                            >  SRR19973067.1797977/1‑150 (MQ=60)
                                          GAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAA                                                                                                           <  SRR19973067.2598928/150‑1 (MQ=60)
                                            AACAATTACGTATGCCGCAAAATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAAT                                                                                                         <  SRR19973067.966510/150‑1 (MQ=60)
                                              CAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGG                                                                                                       >  SRR19973067.471750/1‑150 (MQ=60)
                                              CAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGG                                                                                                       >  SRR19973067.471742/1‑150 (MQ=60)
                                               AATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGA                                                                                                      >  SRR19973067.1923471/1‑150 (MQ=60)
                                                  TACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTG                                                                                                   <  SRR19973067.2293391/150‑1 (MQ=60)
                                                   ACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGT                                                                                                  <  SRR19973067.661243/150‑1 (MQ=60)
                                                   ACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGT                                                                                                  <  SRR19973067.661205/150‑1 (MQ=60)
                                                   ACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGT                                                                                                  <  SRR19973067.1554292/150‑1 (MQ=60)
                                                    CGTATGCCGCAACATGACCCGGCGCTGTTTCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTG                                                                                                 >  SRR19973067.3204109/1‑150 (MQ=60)
                                                         GCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGA                                                                                            <  SRR19973067.2436008/150‑1 (MQ=60)
                                                         GCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGA                                                                                            <  SRR19973067.2436413/150‑1 (MQ=60)
                                                         GCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGA                                                                                            >  SRR19973067.2061312/1‑150 (MQ=60)
                                                          CCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGAT                                                                                           <  SRR19973067.3432350/150‑1 (MQ=60)
                                                           CGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATG                                                                                          >  SRR19973067.2577181/1‑150 (MQ=60)
                                                             CAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTGAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGC                                                                                        <  SRR19973067.1565953/150‑1 (MQ=60)
                                                             CAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGC                                                                                        >  SRR19973067.1611309/1‑150 (MQ=60)
                                                              AACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCTGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCT                                                                                       <  SRR19973067.2940157/150‑1 (MQ=60)
                                                              AACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCT                                                                                       <  SRR19973067.2940145/150‑1 (MQ=60)
                                                                CATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGG                                                                                     >  SRR19973067.3073973/1‑150 (MQ=60)
                                                                  TGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCT                                                                                   <  SRR19973067.3222565/150‑1 (MQ=60)
                                                                   GACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTG                                                                                  <  SRR19973067.1503279/150‑1 (MQ=60)
                                                                             TGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCTCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCG                                                                        >  SRR19973067.2756573/1‑150 (MQ=60)
                                                                             TGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCG                                                                        >  SRR19973067.968472/1‑150 (MQ=60)
                                                                             TGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCG                                                                        <  SRR19973067.1661099/150‑1 (MQ=60)
                                                                             TGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCG                                                                        <  SRR19973067.2186821/150‑1 (MQ=60)
                                                                              GTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGG                                                                       <  SRR19973067.2098419/150‑1 (MQ=60)
                                                                               TTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGG                                                                      <  SRR19973067.854811/150‑1 (MQ=60)
                                                                               TTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGG                                                                      <  SRR19973067.854708/150‑1 (MQ=60)
                                                                               TTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGG                                                                      <  SRR19973067.3030194/150‑1 (MQ=60)
                                                                                TGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGagatc                                                                     >  SRR19973067.2872116/1‑145 (MQ=60)
                                                                                TGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGagatc                                                                     >  SRR19973067.1086401/1‑145 (MQ=60)
                                                                                TGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGC                                                                     >  SRR19973067.373280/1‑150 (MQ=60)
                                                                                TGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGGCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGagatc                                                                     >  SRR19973067.1086388/1‑145 (MQ=60)
                                                                                  CGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCG                                                                   <  SRR19973067.3569572/150‑1 (MQ=60)
                                                                                   GGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGA                                                                  <  SRR19973067.1549784/150‑1 (MQ=60)
                                                                                     CTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATG                                                                <  SRR19973067.1611916/150‑1 (MQ=60)
                                                                                     CTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATG                                                                <  SRR19973067.3347802/150‑1 (MQ=60)
                                                                                     CTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCCGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATG                                                                <  SRR19973067.3347866/150‑1 (MQ=60)
                                                                                      TGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGA                                                               >  SRR19973067.2263378/1‑150 (MQ=60)
                                                                                      TGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGA                                                               <  SRR19973067.1578805/150‑1 (MQ=60)
                                                                                        AATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACA                                                             <  SRR19973067.2241504/150‑1 (MQ=60)
                                                                                        AATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACA                                                             <  SRR19973067.441329/150‑1 (MQ=60)
                                                                                            ATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGC                                                         >  SRR19973067.2080368/1‑150 (MQ=60)
                                                                                              CTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGA                                                       >  SRR19973067.1499985/1‑150 (MQ=60)
                                                                                                CTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACT                                                     <  SRR19973067.3135874/150‑1 (MQ=60)
                                                                                                CTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACT                                                     <  SRR19973067.2005005/150‑1 (MQ=60)
                                                                                                CTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACT                                                     >  SRR19973067.832127/1‑150 (MQ=60)
                                                                                                     CCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATA                                                <  SRR19973067.938592/150‑1 (MQ=60)
                                                                                                          GAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGAC                                           >  SRR19973067.2509331/1‑150 (MQ=60)
                                                                                                          GAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGAC                                           <  SRR19973067.2812374/150‑1 (MQ=60)
                                                                                                                 TTAGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGG                                    >  SRR19973067.2563305/1‑150 (MQ=60)
                                                                                                                   AGCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGAT                                  >  SRR19973067.2455051/1‑150 (MQ=60)
                                                                                                                    GCGCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATG                                 <  SRR19973067.1867518/150‑1 (MQ=60)
                                                                                                                      GCCGTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCG                               <  SRR19973067.548613/150‑1 (MQ=60)
                                                                                                                         GTCAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTG                            >  SRR19973067.535491/1‑150 (MQ=60)
                                                                                                                           CAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGT                          >  SRR19973067.1499157/1‑150 (MQ=60)
                                                                                                                           CAATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGT                          >  SRR19973067.3566652/1‑150 (MQ=60)
                                                                                                                             ATCCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAA                        >  SRR19973067.2457352/1‑150 (MQ=60)
                                                                                                                               CCGAAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAA                      >  SRR19973067.3557529/1‑150 (MQ=60)
                                                                                                                                  AAACAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGAC                   >  SRR19973067.1411703/1‑150 (MQ=60)
                                                                                                                                     CAATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAAT                >  SRR19973067.298187/1‑150 (MQ=60)
                                                                                                                                       ATTCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATAT              <  SRR19973067.578522/150‑1 (MQ=60)
                                                                                                                                         TCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGA            <  SRR19973067.2243774/150‑1 (MQ=60)
                                                                                                                                         TCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGA            <  SRR19973067.2243723/150‑1 (MQ=60)
                                                                                                                                         TCGCCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGA            <  SRR19973067.3432225/150‑1 (MQ=60)
                                                                                                                                           GCCATGTCAGGGGCATTTAGTGGTGCCCTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATCGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACCATATGACT          >  SRR19973067.530181/1‑150 (MQ=60)
                                                                                                                                            CCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTG         >  SRR19973067.1589936/1‑150 (MQ=60)
                                                                                                                                            CCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTG         <  SRR19973067.3001088/150‑1 (MQ=60)
                                                                                                                                            CCATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTG         >  SRR19973067.1590242/1‑150 (MQ=60)
                                                                                                                                             CATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGC        <  SRR19973067.2004290/150‑1 (MQ=60)
                                                                                                                                             CATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGC        >  SRR19973067.1981839/1‑150 (MQ=60)
                                                                                                                                             CATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGC        <  SRR19973067.196776/150‑1 (MQ=60)
                                                                                                                                              ATGTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGCG       <  SRR19973067.1663927/150‑1 (MQ=60)
                                                                                                                                                GTCAGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGCGGG     >  SRR19973067.1027211/1‑150 (MQ=60)
                                                                                                                                                   AGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGCGGGTTC  <  SRR19973067.813202/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
GACTGATGTTAGCGTCGATGTGTTAGGCGGTAACGTACTGATGAAACAATTACGTATGCCGCAACATGACCCGGCGCTGTTGCGGCTGAATAATCTCTCATCCAGCGAACTGGTTAGCGCCGTCAATCCGAAACAATTCGCCATGTCCGGGGCATTTAGTGGTGCACTGCCGTTATGGCTGAACAATGAAAAATGGATTGTGAAAGATGGCTGGCTGGCGAATAGCGGGCCGATGACATTGCGACTGGATAAAGACACTGCGGATGCGGTGGTAAAAGACAATATGACTGCGGGTTC  >  CP009273/1439371‑1439667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑