Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,372,048 C→A 20.9% intergenic (‑38/+181) rplM ← / ← yhcM 50S ribosomal subunit protein L13/putative AFG1‑like family P‑loop ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,372,0480CA20.9% 619.3 / 128.0 243intergenic (‑38/+181)rplM/yhcM50S ribosomal subunit protein L13/putative AFG1‑like family P‑loop ATPase
Reads supporting (aligned to +/- strand):  ref base C (76/115);  new base A (25/27);  total (101/142)
Fisher's exact test for biased strand distribution p-value = 3.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 246 total aligned reads displayed.

GCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAG  >  CP009273/3371900‑3372197
                                                                                                                                                    |                                                                                                                                                     
    aGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCa                                                                                                                                                  <  1:431976/150‑1 (MQ=255)
     ggCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCaa                                                                                                                                                 >  2:1068748/1‑150 (MQ=255)
        gacgaGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACg                                                                                                                                              <  2:2106462/150‑1 (MQ=255)
           gaGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCt                                                                                                                                           <  1:1446763/150‑1 (MQ=255)
           gaGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAAAACCCAAACGTCt                                                                                                                                           >  2:2996234/1‑150 (MQ=255)
           gaGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTATGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGTTGAAAACCCAAAAGTCt                                                                                                                                           >  1:4114971/1‑150 (MQ=255)
             gCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTc                                                                                                                                         <  1:563001/150‑1 (MQ=255)
               cAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCaa                                                                                                                                       >  2:1811539/1‑150 (MQ=255)
               cAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCaa                                                                                                                                       <  2:2898820/150‑1 (MQ=255)
                aGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAAt                                                                                                                                      <  1:3090903/150‑1 (MQ=255)
                   tCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAAttgt                                                                                                                                   <  2:3411704/150‑1 (MQ=255)
                     aGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAAttgttg                                                                                                                                 <  1:283338/150‑1 (MQ=255)
                     aGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAAAACCCAAACGTCTTCAAttgttg                                                                                                                                 <  2:1403288/150‑1 (MQ=255)
                      gTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGa                                                                                                                                <  2:3123/150‑1 (MQ=255)
                      gTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAAAACCCAAACGTCTTCAATTGTTGa                                                                                                                                >  2:3196985/1‑150 (MQ=255)
                       tAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAg                                                                                                                               <  1:3658887/150‑1 (MQ=255)
                        aGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGGTGAgg                                                                                                                              >  1:4390919/1‑150 (MQ=255)
                          ccAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGtt                                                                                                                            <  1:2770311/150‑1 (MQ=255)
                           cAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAAAACACAAACGTCTTCAATTGTTGAGGTTc                                                                                                                           >  2:2102724/1‑150 (MQ=255)
                            aGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTca                                                                                                                          <  2:3916141/150‑1 (MQ=255)
                             gACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTTGTTTAGCTGTAAAAGTTTTAATTAAAATCTTAACCAATAAAAATTTAAACCTTTGTGAACACCCAAACCTCTTCAATTGTTGAGGTTcac                                                                                                                         >  1:3820848/1‑150 (MQ=255)
                             gACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTAATTAAAAGCTTACCCAATAAATATTTACACGTTTGTGAAAACCCCAACGTCTTCAATTGTTTAGGTTcac                                                                                                                         >  1:1537965/1‑150 (MQ=255)
                              aCGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTcaca                                                                                                                        <  2:136469/150‑1 (MQ=255)
                               cGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAAAACCCAAACGTCTTCAATTGTTGAGGTTCacac                                                                                                                       >  2:367161/1‑150 (MQ=255)
                                   ccAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTAAGGTTTATGGTTTAGCTGTAAAATTTTTAATTAAAATCTTACCCAAAAAAAATTTAAAACTTGGTGAACAACCAAACGTCTTCAAATGTTGAGGTTAACACcaca                                                                                                                   >  1:1504264/1‑150 (MQ=255)
                                      gagTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAATTTTTCATTAAAATCATACCCAATAAAAAGTTACACGTTGGTGTACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACaaat                                                                                                                >  1:34862/1‑149 (MQ=255)
                                        gTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTc                                                                                                              <  2:1610539/150‑1 (MQ=255)
                                           ttACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAAAACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCgg                                                                                                           <  1:4435420/150‑1 (MQ=255)
                                             aCCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCa                                                                                                         >  2:85317/1‑150 (MQ=255)
                                             aCCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTATGGTTTAGCTGTAAAATTTTTCATTAAAAGCTTACCCAATAAATAGTTAAACGTTGGTGAACACCCAAACGTCTTCAATTGTTTAGGTTGACACGACAAAGTCCGGCa                                                                                                         >  1:3981455/1‑150 (MQ=255)
                                                     cgacaacaaCATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTAcc                                                                                                 <  1:3039654/147‑1 (MQ=255)
                                                     cgTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTAcc                                                                                                 >  1:1082505/1‑150 (MQ=255)
                                                      gTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTAccc                                                                                                >  2:3077927/1‑150 (MQ=255)
                                                       tCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTGACACGACAAAGTCCGGCAAACCTAcccc                                                                                               >  2:1133610/1‑150 (MQ=255)
                                                       tCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTAcccc                                                                                               <  2:614849/150‑1 (MQ=255)
                                                        caacaaCATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCt                                                                                              <  2:1733665/150‑1 (MQ=255)
                                                        caacaaCATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCt                                                                                              <  2:265208/150‑1 (MQ=255)
                                                        caacaaCATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAAAACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCt                                                                                              <  1:2640291/150‑1 (MQ=255)
                                                          acaacaTACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTc                                                                                            <  1:30887/150‑1 (MQ=255)
                                                          acaacaTACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTc                                                                                            <  1:646502/150‑1 (MQ=255)
                                                           ctacaTACCAGTCGCGTTTTTCGGTGTCTTGTTTAGATGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTCCACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCg                                                                                           <  2:1622431/148‑1 (MQ=255)
                                                            aacaTACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTAACACGACAAAGTACGGCAAACCAACCCCATCGa                                                                                          >  1:3143330/1‑150 (MQ=255)
                                                             acaTACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGaa                                                                                         >  2:2838346/1‑150 (MQ=255)
                                                               aTACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATa                                                                                       <  2:1957200/150‑1 (MQ=255)
                                                               aTACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATa                                                                                       <  1:2589149/150‑1 (MQ=255)
                                                               aTACCAGTCGCGGTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCAACCCCATCGaaaa                                                                                       >  1:1326316/1‑148 (MQ=255)
                                                                tACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACCCGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAg                                                                                      <  2:3917885/150‑1 (MQ=255)
                                                                  ccAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAAAACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGcc                                                                                    >  1:335914/1‑150 (MQ=255)
                                                                   cAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCt                                                                                   <  1:3610044/150‑1 (MQ=255)
                                                                   cAGGCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTGTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCt                                                                                   <  2:4090877/150‑1 (MQ=255)
                                                                     gTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTAt                                                                                 <  1:3219312/150‑1 (MQ=255)
                                                                     gTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAAAACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTAt                                                                                 <  2:46901/150‑1 (MQ=255)
                                                                          gTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAg                                                                            >  2:4296720/1‑150 (MQ=255)
                                                                            tttACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGca                                                                          <  2:2633887/150‑1 (MQ=255)
                                                                             ttACGGTTTCTGGTTTAGCTGTAAAAGTTTTCTTTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGcac                                                                         <  2:311093/150‑1 (MQ=255)
                                                                                cGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGcacaca                                                                      <  2:2026734/150‑1 (MQ=255)
                                                                                cGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGcacaca                                                                      <  1:2334929/150‑1 (MQ=255)
                                                                                  gTTTCTGTTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAAAACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACaaa                                                                    <  2:1031218/150‑1 (MQ=255)
                                                                                   tttCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACaaaa                                                                   <  2:2768386/150‑1 (MQ=255)
                                                                                   tttCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAAAACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACaaaa                                                                   <  1:974561/150‑1 (MQ=255)
                                                                                      cTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAAAACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGt                                                                >  2:725113/1‑150 (MQ=255)
                                                                                         gTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGtttt                                                             >  1:4004731/1‑150 (MQ=255)
                                                                                          tttAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTg                                                            >  2:4040972/1‑150 (MQ=255)
                                                                                           ttAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTgg                                                           >  2:1342799/1‑150 (MQ=255)
                                                                                                tGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGaaaa                                                      >  2:2205341/1‑150 (MQ=255)
                                                                                                tGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAAAACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGaaaa                                                      >  2:1441902/1‑150 (MQ=255)
                                                                                                tGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAAAACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGaaaa                                                      >  2:1202686/1‑150 (MQ=255)
                                                                                                     aaGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACt                                                 >  2:923683/1‑150 (MQ=255)
                                                                                                       gTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACttt                                               <  1:3342830/150‑1 (MQ=255)
                                                                                                          ttCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCtt                                            <  2:4052803/150‑1 (MQ=255)
                                                                                                          ttCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCtt                                            <  2:2705305/150‑1 (MQ=255)
                                                                                                          ttCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAAAACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCtt                                            <  1:367161/150‑1 (MQ=255)
                                                                                                           tCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTg                                           <  1:2973753/150‑1 (MQ=255)
                                                                                                             aTTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTATTGTa                                         >  2:2613933/1‑150 (MQ=255)
                                                                                                             aTTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGACTATTCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTa                                         <  2:3396843/150‑1 (MQ=255)
                                                                                                             aTTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAAAACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTa                                         <  2:125536/150‑1 (MQ=255)
                                                                                                               tAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAAc                                       >  1:1038032/1‑150 (MQ=255)
                                                                                                                aaaaGCTTACCCAATAAATAGTTACACGTTGGTGAAAACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACg                                      >  1:2657231/1‑150 (MQ=255)
                                                                                                                  aaGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTg                                    >  2:3970667/1‑150 (MQ=255)
                                                                                                                    gCTTACCCAATAAATAGTTACACGTTGGTGAAAACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTggg                                  <  2:1572938/150‑1 (MQ=255)
                                                                                                                     cTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTgggg                                 >  1:162067/1‑150 (MQ=255)
                                                                                                                          ccAATAAATAGTTACACGTTGGTGAAAACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCa                            <  1:174550/150‑1 (MQ=255)
                                                                                                                          ccAATAAATAGTTACACGTTGGTGAAAACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCa                            <  1:3572768/150‑1 (MQ=255)
                                                                                                                            aataaataGTTACACGTTGGTGAAAACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAgg                          >  1:274739/1‑150 (MQ=255)
                                                                                                                               aaataGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGAtt                       >  1:3272935/1‑150 (MQ=255)
                                                                                                                                aataGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATta                      >  2:4195741/1‑150 (MQ=255)
                                                                                                                                aataGTTACACGTTGGTGAAAACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATta                      >  2:2256090/1‑150 (MQ=255)
                                                                                                                                 ataGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTACCGTGGGGTCGCAGGATtat                     >  2:4283729/1‑150 (MQ=255)
                                                                                                                                 ataGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGAGtat                     >  1:3422395/1‑150 (MQ=255)
                                                                                                                                 ataGTTACACGTTGGTGAAAACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATtat                     <  2:2502765/150‑1 (MQ=255)
                                                                                                                                         acGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGt             <  1:1016356/150‑1 (MQ=255)
                                                                                                                                         acGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGt             <  1:159440/150‑1 (MQ=255)
                                                                                                                                            ttGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCgg          <  1:2188574/150‑1 (MQ=255)
                                                                                                                                            ttGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCgg          <  2:2542123/150‑1 (MQ=255)
                                                                                                                                             tGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCggg         <  2:3829447/150‑1 (MQ=255)
                                                                                                                                              ggTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCgggg        <  2:4385015/150‑1 (MQ=255)
                                                                                                                                              ggTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCgggg        <  2:1826809/150‑1 (MQ=255)
                                                                                                                                               gTGAAAACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGt       <  2:2366393/150‑1 (MQ=255)
                                                                                                                                                  aaCACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCaa    <  1:2016421/150‑1 (MQ=255)
                                                                                                                                                  aaCACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCaa    >  1:2430512/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAG  >  CP009273/3371900‑3372197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A