Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F159 I0 R1 879 305.9 9588012 96.6% 9262019 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,372,048 C→A 7.9% intergenic (‑38/+181) rplM ← / ← yhcM 50S ribosomal subunit protein L13/putative AFG1‑like family P‑loop ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,372,0480CA 7.9% 870.6 / 35.2 244intergenic (‑38/+181)rplM/yhcM50S ribosomal subunit protein L13/putative AFG1‑like family P‑loop ATPase
Reads supporting (aligned to +/- strand):  ref base C (114/109);  new base A (12/9);  total (126/118)
Fisher's exact test for biased strand distribution p-value = 6.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 253 total aligned reads displayed.

CGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAG  >  CP009273/3371899‑3372197
                                                                                                                                                     |                                                                                                                                                     
 gcgcAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAaca                                                                                                                                                      <  1:397909/150‑1 (MQ=255)
 gcgcAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAaca                                                                                                                                                      >  2:4114147/1‑150 (MQ=255)
   gcAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACAcc                                                                                                                                                    <  2:2943228/150‑1 (MQ=255)
    cAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAAAAccc                                                                                                                                                   >  2:827600/1‑150 (MQ=255)
    cAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAAAAccc                                                                                                                                                   >  2:2948786/1‑150 (MQ=255)
     aGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCa                                                                                                                                                  >  1:354172/1‑150 (MQ=255)
        cgacgaGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTTGTTTAGCTGTAAAAGTTTTCATTAAAACCTTACCCAAAAAATAGTTAAACGTTGGTGAACAccacaac                                                                                                                                               >  1:4121349/1‑145 (MQ=255)
           cgaGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAAAACCCAAACGTc                                                                                                                                            <  2:4367383/150‑1 (MQ=255)
            gaGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCt                                                                                                                                           >  2:3150801/1‑150 (MQ=255)
            gaGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCt                                                                                                                                           >  2:4598659/1‑150 (MQ=255)
            gaGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCt                                                                                                                                           >  2:2771169/1‑150 (MQ=255)
            gaGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCt                                                                                                                                           >  2:3938468/1‑150 (MQ=255)
              gCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTc                                                                                                                                         >  2:3705528/1‑150 (MQ=255)
               ccAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCa                                                                                                                                        <  2:2484094/150‑1 (MQ=255)
                 aGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAAt                                                                                                                                      <  2:2954267/150‑1 (MQ=255)
                        tAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAg                                                                                                                               >  2:3835448/1‑150 (MQ=255)
                           ccAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGtt                                                                                                                            <  2:1056620/150‑1 (MQ=255)
                              gACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGGTTTACGGGTTCTGGGTTAGCGGTAAAAGGTTTCCTTAAAAGCGTACCCACAAAATAGTTACACGGTGGTGAACACCCCAACCTCGTCAATTGGTGAGGGTcac                                                                                                                         >  1:2416279/1‑150 (MQ=255)
                               aCGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTAGAGGTTcaca                                                                                                                        >  2:2427417/1‑150 (MQ=255)
                                 ggCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACg                                                                                                                      <  2:401315/150‑1 (MQ=255)
                                  gCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTGTGGTTTATCTGTAAAAGTTTTAATTAAAAGCTTACCCAATAAATAGTTACAAGGTGGTGAACACCCCAACGTCTTCAATTTTTGTGGTTGACAAGa                                                                                                                     >  1:4419980/1‑150 (MQ=255)
                                   cccAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCCAACGTCTTCAATTGTTGAGGTTCACACGAc                                                                                                                    >  1:207279/1‑150 (MQ=255)
                                   cccAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTAAGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGTACACCCAAAACTCTTCAAATGTTGAGGTTCACAAGAc                                                                                                                    >  1:191679/1‑150 (MQ=255)
                                    ccAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAAAACCCAAACGTCTTCAATTGTTGAGGTTCACACGACa                                                                                                                   >  2:3020577/1‑150 (MQ=255)
                                     cAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACaa                                                                                                                  >  2:1747098/1‑150 (MQ=255)
                                      agagTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACaaa                                                                                                                 >  2:3988967/1‑150 (MQ=255)
                                        agTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGt                                                                                                               <  2:4624222/150‑1 (MQ=255)
                                            ttACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCgg                                                                                                           <  2:3490228/150‑1 (MQ=255)
                                                 ggTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAAc                                                                                                      >  1:4690487/1‑150 (MQ=255)
                                                        tCAACAACAGACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTAcccc                                                                                               <  2:1421192/150‑1 (MQ=255)
                                                         caacaaCATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTAccccg                                                                                              >  1:3374656/1‑149 (MQ=255)
                                                          aacaacATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCtt                                                                                             >  2:4289906/1‑150 (MQ=255)
                                                           acaacaTACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTc                                                                                            <  2:168414/150‑1 (MQ=255)
                                                           acaacaTACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTc                                                                                            <  1:1855305/150‑1 (MQ=255)
                                                           acaacaTACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTc                                                                                            <  1:2375244/150‑1 (MQ=255)
                                                           acaacaTACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGGTACACGTTGGTGAAAACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTc                                                                                            <  2:3061764/150‑1 (MQ=255)
                                                            caacaTACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAAAACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCg                                                                                           <  1:3737133/150‑1 (MQ=255)
                                                             aacaGACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGa                                                                                          <  2:4317406/150‑1 (MQ=255)
                                                               caTACCAGTCGCGGTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCATCGAAt                                                                                        >  2:2808996/1‑150 (MQ=255)
                                                                aTACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCGACCCCTTCGAATa                                                                                       <  1:4726903/150‑1 (MQ=255)
                                                                 tACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAg                                                                                      <  2:2686121/150‑1 (MQ=255)
                                                                 tACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAg                                                                                      <  1:536071/150‑1 (MQ=255)
                                                                    cAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCt                                                                                   <  2:1243148/150‑1 (MQ=255)
                                                                    cAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCt                                                                                   <  2:4720600/150‑1 (MQ=255)
                                                                         gcgTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAAAACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGcc                                                                              >  1:1674975/1‑150 (MQ=255)
                                                                          cgTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCa                                                                             <  1:2358408/150‑1 (MQ=255)
                                                                           gTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAg                                                                            >  1:818126/1‑150 (MQ=255)
                                                                           gTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAg                                                                            <  1:225337/150‑1 (MQ=255)
                                                                              ttACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGcac                                                                         >  1:3174905/1‑150 (MQ=255)
                                                                              ttACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTAAATTGTTGAGGTTCACACGACAAAGTCAGGCAAACCTACCCCTACGAATATCCTATGCCAGcac                                                                         >  1:2378644/1‑150 (MQ=255)
                                                                                   gTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACaaa                                                                    >  2:1032599/1‑150 (MQ=255)
                                                                                    tttCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACaaaa                                                                   >  2:3099238/1‑150 (MQ=255)
                                                                                       cTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGt                                                                <  1:2086932/150‑1 (MQ=255)
                                                                                        tGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGtt                                                               <  1:879683/150‑1 (MQ=255)
                                                                                             tAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTggg                                                          >  2:870158/1‑150 (MQ=255)
                                                                                             tAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTggg                                                          >  2:870183/1‑150 (MQ=255)
                                                                                              aGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGa                                                         <  2:3634567/150‑1 (MQ=255)
                                                                                              aGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGa                                                         >  1:3103128/1‑150 (MQ=255)
                                                                                              aGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGa                                                         >  1:3712760/1‑150 (MQ=255)
                                                                                                cTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGaaa                                                       <  1:3983112/150‑1 (MQ=255)
                                                                                                 tGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGaaaa                                                      <  2:4470604/150‑1 (MQ=255)
                                                                                                  gTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGaaaaa                                                     <  2:4436373/150‑1 (MQ=255)
                                                                                                    aaaaGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGaaaaaaa                                                   >  2:3330165/1‑150 (MQ=255)
                                                                                                        gTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACttt                                               >  2:2010709/1‑150 (MQ=255)
                                                                                                        gTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACttt                                               >  2:4122011/1‑150 (MQ=255)
                                                                                                            tCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAAGAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTg                                           <  2:3774852/150‑1 (MQ=255)
                                                                                                               ttAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTaa                                        >  2:641293/1‑150 (MQ=255)
                                                                                                               ttAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAAAACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTaa                                        >  1:883938/1‑150 (MQ=255)
                                                                                                                tAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAAc                                       >  2:221737/1‑150 (MQ=255)
                                                                                                                 aaaaGCTTACCCAATAAATAGTTACACGTTGGAGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAAAAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACg                                      >  2:3589181/1‑150 (MQ=255)
                                                                                                                      cTTACCCAATAAATAGTTACACGTTGGTGAAAACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTgggg                                 <  1:3265264/150‑1 (MQ=255)
                                                                                                                        gaCCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTc                               <  2:3482000/149‑1 (MQ=255)
                                                                                                                         aCCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCg                              >  2:1805751/1‑150 (MQ=255)
                                                                                                                         aCCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCg                              <  1:1707905/150‑1 (MQ=255)
                                                                                                                              ataaataGTTACACGTTGGTGAAAACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGa                         >  1:4752119/1‑150 (MQ=255)
                                                                                                                                aaataGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGAtt                       <  2:91172/150‑1 (MQ=255)
                                                                                                                                aaataGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGAtt                       >  2:2623445/1‑150 (MQ=255)
                                                                                                                                aaataGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGAtt                       <  1:3462590/150‑1 (MQ=255)
                                                                                                                                 aataGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATta                      >  1:1592567/1‑150 (MQ=255)
                                                                                                                                 aataGTTACACGTTGGTGAAAACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATta                      <  1:3012854/150‑1 (MQ=255)
                                                                                                                                  ataGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATtat                     <  1:3619883/150‑1 (MQ=255)
                                                                                                                                  ataGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATtat                     >  1:2551282/1‑150 (MQ=255)
                                                                                                                                   taGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATtata                    <  1:532603/150‑1 (MQ=255)
                                                                                                                                    aGTTACACGTTGGTGAACACCCAAACGTCTTCAATTTTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATag                   <  1:1448491/150‑1 (MQ=255)
                                                                                                                                     gTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAAGAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATaga                  <  2:655923/150‑1 (MQ=255)
                                                                                                                                      ttACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATagag                 >  1:1157365/1‑150 (MQ=255)
                                                                                                                                      ttACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATagag                 >  1:624023/1‑150 (MQ=255)
                                                                                                                                      ttACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATagag                 >  1:624709/1‑150 (MQ=255)
                                                                                                                                       tACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAgaga                <  1:2821679/150‑1 (MQ=255)
                                                                                                                                       tACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAgaga                <  1:3828348/150‑1 (MQ=255)
                                                                                                                                         cacGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAg              <  2:3269541/150‑1 (MQ=255)
                                                                                                                                         cacGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAg              >  2:708067/1‑150 (MQ=255)
                                                                                                                                         cacGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAg              >  2:708661/1‑150 (MQ=255)
                                                                                                                                         cacGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAg              >  1:4626910/1‑150 (MQ=255)
                                                                                                                                          acGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGt             <  1:3777002/150‑1 (MQ=255)
                                                                                                                                          acGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGt             >  1:1608656/1‑150 (MQ=255)
                                                                                                                                           cGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAAGAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTc            <  2:1715622/150‑1 (MQ=255)
                                                                                                                                               ggTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATag                   >  1:4216805/1‑139 (MQ=255)
                                                                                                                                                gTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGt       <  1:921624/150‑1 (MQ=255)
                                                                                                                                                     cacCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAg  <  2:1826820/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGCGCAGGCGACGAGCCAGTTCAGTAGCCAGACGGCCCAGAGTTTTACCGGTCGCGTCAACAACATACCAGTCGCGTTTTACGGTTTCTGGTTTAGCTGTAAAAGTTTTCATTAAAAGCTTACCCAATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAG  >  CP009273/3371899‑3372197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A