Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 361,692 G→T 48.4% Q18K (CAG→AAG)  lacI ← DNA‑binding transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273361,6920GT48.4% 4442.0 / inf 7804Q18K (CAG→AAG) lacIDNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (1988/2038);  new base T (1868/1907);  total (3859/3945)
Fisher's exact test for biased strand distribution p-value = 9.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 7899 total aligned reads displayed.

CAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGC  >  CP009273/361545‑361839
                                                                                                                                                     |                                                                                                                                                   
cAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTg                                                                                                                                                     <  2:222860/148‑1 (MQ=255)
  aCGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AACACGGTCTGa                                                                                                                                                    >  2:2833191/1‑148 (MQ=255)
   cGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTtata                                                                                                                                                  >  2:880972/1‑148 (MQ=255)
   cGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTtata                                                                                                                                                  >  1:1180553/1‑148 (MQ=255)
   cGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTGATa                                                                                                                                                  >  2:1606536/1‑148 (MQ=255)
        aTCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTGATAAgaga                                                                                                                                             <  2:1718835/148‑1 (MQ=255)
         tCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTTATAAGAGac                                                                                                                                            >  1:2265436/1‑148 (MQ=255)
         tCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTGATAAGAGac                                                                                                                                            >  1:438739/1‑148 (MQ=255)
         tCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTGATAAGAGac                                                                                                                                            >  2:541142/1‑148 (MQ=255)
          cAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTTATAAGAGaca                                                                                                                                           >  1:1986691/1‑148 (MQ=255)
          cAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTTATAAGAGaca                                                                                                                                           >  1:375149/1‑148 (MQ=255)
          cAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTTATAAGAGaca                                                                                                                                           <  2:2432567/148‑1 (MQ=255)
            gCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTGATAAGAGACAcc                                                                                                                                         >  1:169371/1‑148 (MQ=255)
               aCGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTTATAAGAGACACCGGc                                                                                                                                      >  2:1180738/1‑148 (MQ=255)
                   cTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTCATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTGATAAGAGACACCGGCATAc                                                                                                                                  >  1:3165771/1‑148 (MQ=255)
                   cTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTGATAAGAGACACCGGCATAc                                                                                                                                  >  2:2958370/1‑148 (MQ=255)
                    tGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTTATAAGAGACACCGGCATAct                                                                                                                                 <  2:2071045/148‑1 (MQ=255)
                     gTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTTATAAGAGACACCGGCATActc                                                                                                                                <  1:2629831/148‑1 (MQ=255)
                     gTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTGATAAGAGACACCGGCATActc                                                                                                                                >  1:2686799/1‑148 (MQ=255)
                       ttGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTCATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTGATAAGAGACACCGGCATACTCTg                                                                                                                              >  2:2484280/1‑148 (MQ=255)
                       ttGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTTATAAGAGACACCGGCATACTCTg                                                                                                                              <  1:995673/148‑1 (MQ=255)
                       ttGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTTATAAGAGACACCGGCATACTCTg                                                                                                                              <  1:2558076/148‑1 (MQ=255)
                              ccAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTGCCGCGTTTTCGCAGAAACGTGGCTGGCCGGGTTCACCACGCGGG‑AA‑ACGGTCTTATAAGAGACACCGGCATACTCTGCGACATc                                                                                                                       >  1:599809/1‑148 (MQ=255)
                              ccAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTTATAAGAGACACCGGCATACTCTGCGACATc                                                                                                                       >  1:2449619/1‑148 (MQ=255)
                                aGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGt                                                                                                                     <  2:1772298/148‑1 (MQ=255)
                                aGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACATTTTCCCGCGTTTTGGCAGAAACCAGGCTGGCCGGGGTCACCACCCGGG‑AC‑ACGCGGTGATAAGAGACACCGGCATACACTGCGACATCGt                                                                                                                     >  1:1133134/1‑148 (MQ=255)
                                 gttgttGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAA‑ACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGt                                                                                                                     <  2:143777/148‑1 (MQ=255)
                                   tgttgtGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGtata                                                                                                                  <  2:822905/148‑1 (MQ=255)
                                    gttgtGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATaa                                                                                                                 <  2:1947416/148‑1 (MQ=255)
                                     ttgtGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAAc                                                                                                                >  1:186444/1‑148 (MQ=255)
                                      tgtgCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACg                                                                                                               >  2:2010762/1‑148 (MQ=255)
                                      tgtgCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTGATAAGAGACACCGGTATACTCTGCGACATCGTATAACg                                                                                                               <  1:1058894/148‑1 (MQ=255)
                                      tgtgCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACg                                                                                                               <  1:3105200/148‑1 (MQ=255)
                                             cgcgGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGACACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTgg                                                                                                        >  2:165992/1‑148 (MQ=255)
                                             cgcgGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTACAAGTTTTCCCGCGTTTTCGCAGAAAAGTTGCTGGCCTGGTTCAACACCCGGG‑AA‑AAGGTCTTATAATAGACACCGGCATACACTGCGACAACGTATAAAGTTAATgg                                                                                                        >  1:7791/1‑148 (MQ=255)
                                              gcgGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGt                                                                                                       <  1:3230366/148‑1 (MQ=255)
                                              gcgGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGt                                                                                                       <  2:2302614/148‑1 (MQ=255)
                                                ggTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGttt                                                                                                     <  2:2664643/148‑1 (MQ=255)
                                                    gggAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTTATAAGAGACACCGGCCTACTCTGCGACATCGTATAACGTTACTGGTTTcaca                                                                                                 >  1:88378/1‑148 (MQ=255)
                                                     ggAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACCCGGG‑AA‑ACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACAt                                                                                                >  1:651249/1‑148 (MQ=255)
                                                      gAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACAtt                                                                                               <  1:209573/148‑1 (MQ=255)
                                                         tGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTcac                                                                                            >  2:1058063/1‑148 (MQ=255)
                                                          gTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTcacc                                                                                           >  1:552421/1‑148 (MQ=255)
                                                             aTTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCaccacc                                                                                        <  1:1375559/148‑1 (MQ=255)
                                                              ttCAGCTCCGCCATCGCCGCTTCCACATTTTACCGCGTTTTGGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑CA‑ACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCAccc                                                                                       >  1:2500580/1‑148 (MQ=255)
                                                               tCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCt                                                                                      >  1:2746957/1‑148 (MQ=255)
                                                               tCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCt                                                                                      <  2:2116592/148‑1 (MQ=255)
                                                               tCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCt                                                                                      >  2:656182/1‑148 (MQ=255)
                                                                  gCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAA‑ACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCGGa                                                                                    >  2:2774831/1‑148 (MQ=255)
                                                                   cTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAAt                                                                                  >  1:2976876/1‑148 (MQ=255)
                                                                     ccGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAA‑ACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAAtt                                                                                 >  2:941028/1‑148 (MQ=255)
                                                                        ccATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGAct                                                                             <  1:1000229/148‑1 (MQ=255)
                                                                         cATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGGGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCAGATAAGAGACACCGGCATACTCTGCGACAGCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGActc                                                                            >  2:1247764/1‑148 (MQ=255)
                                                                           tcgccgcTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAA‑ACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGActct                                                                           <  1:715107‑M2/141‑1 (MQ=255)
                                                                             gccgcTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCtt                                                                        <  2:766245‑M2/143‑1 (MQ=255)
                                                                             gccgcTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCtt                                                                        <  1:2320369‑M2/143‑1 (MQ=255)
                                                                                gcTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCg                                                                     >  2:48183‑M2/3‑148 (MQ=255)
                                                                                   tCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGgc                                                                  >  1:831093/1‑148 (MQ=255)
                                                                                   tCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGgc                                                                  >  2:70906/1‑148 (MQ=255)
                                                                                     cACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGgcgc                                                                <  2:1381682/148‑1 (MQ=255)
                                                                                      aCTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCTCCACGCGGG‑AA‑ACGGTCTGATAAGAGACACCGGCATACTCTGTGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCt                                                               <  2:3026595/148‑1 (MQ=255)
                                                                                      aCTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCt                                                               <  2:2711712/148‑1 (MQ=255)
                                                                                       cTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTa                                                              >  1:2047163/1‑148 (MQ=255)
                                                                                        tttttCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTAt                                                             <  2:1544011/148‑1 (MQ=255)
                                                                                         ttttCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATc                                                            <  1:1918326/148‑1 (MQ=255)
                                                                                         ttttCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATc                                                            <  2:3211528/148‑1 (MQ=255)
                                                                                         ttttCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATc                                                            <  1:2150698/148‑1 (MQ=255)
                                                                                                   ttttCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAA‑ACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATAc                                                   >  2:3154498/1‑148 (MQ=255)
                                                                                                    tttCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACcg                                                 >  2:1495689/1‑148 (MQ=255)
                                                                                                    tttCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACcg                                                 >  2:2167461/1‑148 (MQ=255)
                                                                                                    tttCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACcg                                                 >  1:499840/1‑148 (MQ=255)
                                                                                                     ttCGCAGAAACGTGGCTGGGCTGGTTCACCACGCGGT‑AA‑ACGGTCTGTTAAGAGACACCGTCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACcgg                                                <  2:2890166/148‑2 (MQ=255)
                                                                                                        gCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGaa                                             <  2:1306824/148‑1 (MQ=255)
                                                                                                           gAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAgg                                          >  1:948220/1‑148 (MQ=255)
                                                                                                           gAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAgg                                          <  1:2816601/148‑1 (MQ=255)
                                                                                                            aaaCGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGt                                         >  2:59322/1‑148 (MQ=255)
                                                                                                            aaaCGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGt                                         <  1:1578653/148‑1 (MQ=255)
                                                                                                             aaCGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGtt                                        <  1:3035501/148‑1 (MQ=255)
                                                                                                               cGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGtttt                                      >  1:1452621/1‑148 (MQ=255)
                                                                                                                  ggctggcCTGGTTCACCACGCGGG‑AA‑ACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTgca                                   <  2:3276036/148‑2 (MQ=255)
                                                                                                                      ggcCTGGTTCACCACGCGGG‑AA‑ACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCAt                               >  2:350510/1‑148 (MQ=255)
                                                                                                                       gcCTGGGTCACCACGCGGG‑AA‑ACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCAtt                              <  1:1251225/148‑1 (MQ=255)
                                                                                                                          tGGTTCACCACGCGGG‑AA‑ACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGa                           <  2:3092434/148‑1 (MQ=255)
                                                                                                                           ggTTCACCACGCGGG‑AA‑ACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGAt                          >  1:1808466/1‑148 (MQ=255)
                                                                                                                            gTTCACCACGCGGG‑AA‑ACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATg                         <  2:2595419/148‑1 (MQ=255)
                                                                                                                             ttCACCACGCGGG‑AA‑ACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATgg                        <  1:2388132/148‑1 (MQ=255)
                                                                                                                              tCACCACGCGGG‑AA‑ACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACAGTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTAGATGgt                       <  2:1887679/148‑1 (MQ=255)
                                                                                                                               caccacGCGGG‑AA‑ACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGgtg                      <  1:220875/148‑1 (MQ=255)
                                                                                                                                accacGCGGG‑AA‑ACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGgtgt                     <  2:1127179/148‑1 (MQ=255)
                                                                                                                                 ccacGCGGG‑AA‑ACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTc                    >  1:1753377/1‑148 (MQ=255)
                                                                                                                                 ccacGCGGG‑AA‑ACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTc                    <  1:1631505/148‑1 (MQ=255)
                                                                                                                                  cacGCGGG‑AA‑ACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCa                   >  1:1317369/1‑148 (MQ=255)
                                                                                                                                  cacGCGGG‑AA‑ACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCa                   <  2:1837243/148‑1 (MQ=255)
                                                                                                                                      cgGG‑AA‑ACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAtta               <  1:3293529‑M2/148‑4 (MQ=255)
                                                                                                                                        gg‑AA‑ACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaat             >  2:417565‑M2/1‑143 (MQ=255)
                                                                                                                                        gg‑AA‑ACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaat             <  2:2361784‑M2/148‑6 (MQ=255)
                                                                                                                                            a‑aCGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaa          >  1:1514270‑M2/1‑140 (MQ=255)
                                                                                                                                               cGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaag        <  1:2869297‑M2/148‑11 (MQ=255)
                                                                                                                                                ggTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaagg       >  2:25328‑M2/1‑137 (MQ=255)
                                                                                                                                                  tCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCAGTCGATGGTGTCAAttaattaaaggca     <  1:2930581‑M2/148‑14 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AA‑ACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGC  >  CP009273/361545‑361839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A