Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 361,692 G→T 38.2% Q18K (CAG→AAG)  lacI ← DNA‑binding transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273361,6920GT38.2% 11431.4 / inf 8496Q18K (CAG→AAG) lacIDNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (2438/2807);  new base T (1575/1673);  total (4016/4480)
Fisher's exact test for biased strand distribution p-value = 7.36e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 8637 total aligned reads displayed.

CAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGC  >  CP009273/361545‑361836
                                                                                                                                                    |                                                                                                                                                
  aCGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAAACGGTCTGa                                                                                                                                                 >  1:682030/1‑148 (MQ=255)
   cGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTCATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATa                                                                                                                                               >  2:2840990/1‑148 (MQ=255)
   cGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAAAGGTCTGATa                                                                                                                                               >  1:3070726/1‑148 (MQ=255)
   cGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCGGGTTCACCACGCGGG‑AAACGGTCTGATa                                                                                                                                               >  2:732419/1‑148 (MQ=255)
     ccAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCTCCACGCGGG‑AAACGGTCTGATAag                                                                                                                                             <  2:215037/148‑1 (MQ=255)
     ccAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑TCACGGTCTGATAag                                                                                                                                             >  1:1571562/1‑148 (MQ=255)
        aTCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAgaga                                                                                                                                          >  1:1215099/1‑148 (MQ=255)
        aTCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTGTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAgaga                                                                                                                                          <  1:2114973/148‑1 (MQ=255)
         tCAGCAACGACAGTTTGCCCGCCAGTTGTAGTGCCACGCGGATGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGac                                                                                                                                         <  2:3427333/148‑1 (MQ=255)
          cAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGCAAACGGTCGGATAAGAGac                                                                                                                                         >  1:1000396/1‑148 (MQ=255)
             cAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTTATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGacac                                                                                                                                       >  2:3500734/1‑146 (MQ=255)
                cGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCa                                                                                                                                  >  1:2803639/1‑148 (MQ=255)
                  aCTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATa                                                                                                                                >  1:3487161/1‑148 (MQ=255)
                  aCTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATa                                                                                                                                <  2:785715/148‑1 (MQ=255)
                   cTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACGTTTTCCCGCGTTTTCGCAGAAAAGTGGCTGGCCTGGTTCACCACGCGGG‑AAACCGTCTTATAAGAGACACCGGCATAc                                                                                                                               >  1:9967/1‑148 (MQ=255)
                    tGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATAct                                                                                                                              >  1:1855155/1‑148 (MQ=255)
                     gTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATActc                                                                                                                             >  1:1148435/1‑148 (MQ=255)
                      tttGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATActct                                                                                                                            >  2:658882/1‑148 (MQ=255)
                       ttGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTg                                                                                                                           >  1:2465753/1‑148 (MQ=255)
                             gCCAGTTGTTGTGCCACGTGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACAt                                                                                                                     <  2:3351609/148‑1 (MQ=255)
                              ccAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAACCGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCGTATAAGCGACACCGGCCTAGTCTGCGACATc                                                                                                                    >  2:3634864/1‑148 (MQ=255)
                               cAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCg                                                                                                                   <  2:1887341/148‑1 (MQ=255)
                               cAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCg                                                                                                                   >  2:1571640/1‑148 (MQ=255)
                                aGTTGTTGTGCCACGCGGTTGGGAATGTCATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGt                                                                                                                  <  1:2927667/148‑1 (MQ=255)
                                aGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCTCCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGt                                                                                                                  <  2:2702736/148‑1 (MQ=255)
                                 gttgtaGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGta                                                                                                                 <  2:2769774/148‑1 (MQ=255)
                                  ttgttgTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTGCCGCGTTTTAGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACACAGCGACATCGtat                                                                                                                >  1:752677/1‑148 (MQ=255)
                                  ttgttgTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGtat                                                                                                                >  2:1282149/1‑148 (MQ=255)
                                  ttgttgTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGtat                                                                                                                >  2:170849/1‑148 (MQ=255)
                                  ttgttgTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTACCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑GACCGGGCGGATAAGAGACACCGGCCTACTCTGCGACATCGtct                                                                                                                >  1:2583564/1‑146 (MQ=255)
                                     ttgtGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAAc                                                                                                             >  2:2985531/1‑148 (MQ=255)
                                         gCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTa                                                                                                         <  1:2387707/148‑1 (MQ=255)
                                            aCGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCTCCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTg                                                                                                      <  2:202572/148‑1 (MQ=255)
                                             cgcgGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTgg                                                                                                     <  2:1439442/148‑1 (MQ=255)
                                                  ttGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTc                                                                                                 >  2:2578518/1‑148 (MQ=255)
                                                  ttGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGtttaa                                                                                                >  1:252770/1‑146 (MQ=255)
                                                  ttGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTca                                                                                                <  1:2863523/148‑1 (MQ=255)
                                                  ttGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCAATTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTca                                                                                                >  2:429460/1‑148 (MQ=255)
                                                   tGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCTCGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTcac                                                                                               <  1:1980144/148‑1 (MQ=255)
                                                   tGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTcac                                                                                               >  1:2324669/1‑148 (MQ=255)
                                                   tGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTcac                                                                                               >  1:1917685/1‑148 (MQ=255)
                                                     ggAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACAt                                                                                             >  1:2673094/1‑148 (MQ=255)
                                                     ggAATGTAAGTCAGCTCCGCCATCGCCGCTTCCACTTTGTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACAt                                                                                             <  2:1365497/148‑1 (MQ=255)
                                                      gAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTATCACAtt                                                                                            <  1:337524/148‑1 (MQ=255)
                                                         tGTAATTCAGCTCCGCCATCGCAGCTTCCACTTTTTCACGCGTTTTATCAGAAACGTTGGTGGCCTGGTTAACAACCAGGG‑AAACCGTCTGATAAGAAACAACGGCATAATCTTCGACATCGTATAACGTTAATTGTTTCAAATTcac                                                                                         >  1:3561321/1‑148 (MQ=255)
                                                            aaTTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAAACGGGCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTcacca                                                                                       >  1:604659/1‑148 (MQ=255)
                                                              ttCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCAccc                                                                                    <  2:1500082/148‑1 (MQ=255)
                                                               tCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCAccc                                                                                    >  1:2242922/1‑148 (MQ=255)
                                                               tCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCt                                                                                   >  2:1963960/1‑148 (MQ=255)
                                                                  gCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTAGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACAACCTATAACGTTACAGGTTTCACATAAACCACCCTGaa                                                                                >  1:3303302/1‑148 (MQ=255)
                                                                   cTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAAt                                                                               <  2:1763106/148‑1 (MQ=255)
                                                                    tCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAAtt                                                                              <  1:2047687/148‑1 (MQ=255)
                                                                      cGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCTCCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGa                                                                            <  1:1504451/148‑1 (MQ=255)
                                                                      cGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGa                                                                            <  2:2637918/148‑1 (MQ=255)
                                                                         cATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGActc                                                                         >  2:1157511/1‑148 (MQ=255)
                                                                         cATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGActc                                                                         <  2:610182/148‑1 (MQ=255)
                                                                          aTCGCCGCTTCCACTTTTTACCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCAACAAGCGGG‑GAACGGTCTTATAAGATACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTAACCACCCTGAATTTActct                                                                        >  1:844037/1‑148 (MQ=255)
                                                                           tCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCTCCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGActctc                                                                       <  2:1071093/148‑1 (MQ=255)
                                                                            cgccgcTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGActctct                                                                      <  2:1832565/148‑1 (MQ=255)
                                                                             gccgcTTCCACTATTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCTCCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGTGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCtt                                                                     <  2:2084794/148‑1 (MQ=255)
                                                                               cgcTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTcc                                                                   >  1:136713/1‑148 (MQ=255)
                                                                                 cTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCgg                                                                 >  2:487706/1‑148 (MQ=255)
                                                                                   tCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGgc                                                               >  1:1072367/1‑148 (MQ=255)
                                                                                      aCTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTCTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCt                                                            <  1:3626279/148‑1 (MQ=255)
                                                                                      aCTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTATCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCt                                                            >  1:2741680/1‑148 (MQ=255)
                                                                                       cTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTa                                                           >  2:3055014/1‑148 (MQ=255)
                                                                                        tttttCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTAt                                                          >  2:122473/1‑148 (MQ=255)
                                                                                        tttttCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTAt                                                          >  1:2995304/1‑148 (MQ=255)
                                                                                             cccGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGc                                                     >  2:941788/1‑148 (MQ=255)
                                                                                               cgcgTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCa                                                   >  2:3276936/1‑148 (MQ=255)
                                                                                                 cgTTTTCGCAGAAACGTGGCTGGCCTGGTTCTCCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATa                                                 >  2:545136/1‑148 (MQ=255)
                                                                                                 cgTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATa                                                 <  1:3230289/148‑1 (MQ=255)
                                                                                                     ttCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACcgc                                             >  1:1296819/1‑148 (MQ=255)
                                                                                                            aaaCGTGGCTGGCCTGGTTCTCCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGt                                      <  2:2232938/148‑1 (MQ=255)
                                                                                                            aaaCGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGt                                      >  1:3759445/1‑148 (MQ=255)
                                                                                                            aaaCGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGt                                      >  2:868286/1‑148 (MQ=255)
                                                                                                            aaaCGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGt                                      >  2:2516067/1‑148 (MQ=255)
                                                                                                             aaCGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGtt                                     <  1:470749/148‑1 (MQ=255)
                                                                                                              aCGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGttt                                    >  2:320093/1‑148 (MQ=255)
                                                                                                              aCGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGttt                                    <  1:2185338/148‑1 (MQ=255)
                                                                                                                  ggctggcCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTgca                                <  1:1790756/148‑2 (MQ=255)
                                                                                                                   gctggcCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTgcac                               >  1:395469/1‑146 (MQ=255)
                                                                                                                      ggcCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCAt                            <  2:2304205/148‑1 (MQ=255)
                                                                                                                       gcCTGGTTCTCCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCAtt                           >  1:3145747/1‑148 (MQ=255)
                                                                                                                       gcCTGGTTCACCACGCGGGAAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCAt                            <  2:3736829/148‑1 (MQ=255)
                                                                                                                       gcCTGGTTCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCAtt                           >  1:909939/1‑148 (MQ=255)
                                                                                                                              tctccacGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGgt                    <  1:1724303/145‑1 (MQ=255)
                                                                                                                              tCACCACGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGgt                    >  1:1642617/1‑148 (MQ=255)
                                                                                                                              tCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGgt                    >  2:2903025/1‑148 (MQ=255)
                                                                                                                                  cacGCGGG‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCa                >  1:540071/1‑148 (MQ=255)
                                                                                                                                     gcgGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAtt             >  1:3431562‑M2/1‑146 (MQ=255)
                                                                                                                                       ggg‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaa           >  1:1440841‑M2/1‑144 (MQ=255)
                                                                                                                                       ggg‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaa           >  2:3806236‑M2/1‑144 (MQ=255)
                                                                                                                                        gg‑AAACGGTCTTATCAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaat          >  2:2376022‑M2/1‑143 (MQ=255)
                                                                                                                                        gg‑AAACGGTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaat          >  2:3129001‑M2/1‑143 (MQ=255)
                                                                                                                                          aaaacGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaatt         <  2:1633709‑M2/147‑7 (MQ=255)
                                                                                                                                           aaaCGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaatta        >  2:3696133‑M2/1‑141 (MQ=255)
                                                                                                                                             aCGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaa      >  1:3698114‑M2/1‑139 (MQ=255)
                                                                                                                                                gTCTTATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggc   >  2:3562921‑M2/1‑136 (MQ=255)
                                                                                                                                                gTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggc   <  2:2449863‑M2/148‑13 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
CAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGG‑AAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGC  >  CP009273/361545‑361836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A