Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 450,766 G→T 90.8% A60S (GCG→TCG)  tig → peptidyl‑prolyl cis/trans isomerase (trigger factor)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273450,7660GT90.8% 461.4 / 40.8 175A60S (GCG→TCG) tigpeptidyl‑prolyl cis/trans isomerase (trigger factor)
Reads supporting (aligned to +/- strand):  ref base G (11/5);  new base T (72/87);  total (83/92)
Fisher's exact test for biased strand distribution p-value = 1.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

Only 100 of 176 total aligned reads displayed.

TGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTGTAG  >  CP009273/450621‑450910
                                                                                                                                                 |                                                                                                                                                
tGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGctcg                                                                                                                                                >  2:253406/1‑148 (MQ=255)
          gtAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGcc                                                                                                                                      >  2:2671850/1‑148 (MQ=255)
          gtAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGcc                                                                                                                                      >  1:1492297/1‑148 (MQ=255)
           tAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCa                                                                                                                                     <  1:342801/148‑1 (MQ=255)
            aaCGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAg                                                                                                                                    >  1:1585493/1‑148 (MQ=255)
              cGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGa                                                                                                                                  <  1:486612/148‑1 (MQ=255)
              cGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGa                                                                                                                                  <  1:486020/148‑1 (MQ=255)
                 ttACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGt                                                                                                                               >  1:2310001/1‑148 (MQ=255)
                  tACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGtt                                                                                                                              <  2:3252240/148‑1 (MQ=255)
                  tACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGtt                                                                                                                              <  2:3252194/148‑1 (MQ=255)
                  tACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGtt                                                                                                                              <  2:1566398/148‑1 (MQ=255)
                  tACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGtt                                                                                                                              >  2:3050041/1‑148 (MQ=255)
                        cGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGt                                                                                                                        >  1:1236176/1‑148 (MQ=255)
                        cGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGt                                                                                                                        >  1:2886001/1‑148 (MQ=255)
                         gctgctGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTg                                                                                                                       <  2:1615373/148‑1 (MQ=255)
                               gACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCtga                                                                                                                 <  2:1746763/148‑1 (MQ=255)
                               gACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCtga                                                                                                                 >  2:2894592/1‑148 (MQ=255)
                               gACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCtga                                                                                                                 >  1:1416954/1‑148 (MQ=255)
                               gACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCtga                                                                                                                 >  1:1414085/1‑148 (MQ=255)
                               gACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCtga                                                                                                                 >  1:3237298/1‑148 (MQ=255)
                                 cAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCtgatg                                                                                                               >  2:2292492/1‑148 (MQ=255)
                                   gCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAg                                                                                                             >  2:1809463/1‑148 (MQ=255)
                                   gCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAg                                                                                                             <  1:1299910/148‑1 (MQ=255)
                                    cATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGc                                                                                                            <  2:196556/148‑1 (MQ=255)
                                     aTCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGcc                                                                                                           >  1:967231/1‑148 (MQ=255)
                                       cGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGt                                                                                                         <  1:669822/148‑1 (MQ=255)
                                        gagaCCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTa                                                                                                        <  2:2211278/148‑1 (MQ=255)
                                        gagaCCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTa                                                                                                        <  1:418316/148‑1 (MQ=255)
                                         agaCCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTaa                                                                                                       >  2:1220382/1‑148 (MQ=255)
                                         agaCCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTaa                                                                                                       >  2:1220357/1‑148 (MQ=255)
                                         agaCCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTaa                                                                                                       <  2:415336/148‑1 (MQ=255)
                                          gaCCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAAc                                                                                                      >  2:287327/1‑148 (MQ=255)
                                          gaCCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAAc                                                                                                      >  1:3111834/1‑148 (MQ=255)
                                           aCCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACt                                                                                                     >  2:3111755/1‑148 (MQ=255)
                                             cGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTc                                                                                                   <  1:2858821/148‑1 (MQ=255)
                                                 gTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTg                                                                                               <  2:1027804/148‑1 (MQ=255)
                                                 gTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTg                                                                                               <  2:579993/148‑1 (MQ=255)
                                                 gTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTg                                                                                               <  2:2202485/148‑1 (MQ=255)
                                                  ttAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGa                                                                                              <  1:1228552/148‑1 (MQ=255)
                                                     aaaGCTAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGc                                                                                           <  1:2978540/148‑1 (MQ=255)
                                                     aaaGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGc                                                                                           >  1:248383/1‑148 (MQ=255)
                                                          gAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCcatca                                                                                      <  1:974218/148‑1 (MQ=255)
                                                          gAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCcatca                                                                                      <  1:974128/148‑1 (MQ=255)
                                                           aGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCcatcat                                                                                     >  2:3181599/1‑148 (MQ=255)
                                                           aGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCcatcat                                                                                     <  2:74242/148‑1 (MQ=255)
                                                           aGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCcatcat                                                                                     >  2:961778/1‑148 (MQ=255)
                                                           aGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCcatcat                                                                                     <  1:338068/148‑1 (MQ=255)
                                                             cTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTa                                                                                   >  2:1969009/1‑148 (MQ=255)
                                                              tGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTaa                                                                                  <  2:1644626/148‑1 (MQ=255)
                                                               ggTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTaaa                                                                                 <  1:2026311/148‑1 (MQ=255)
                                                               ggTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTaaa                                                                                 <  2:1447303/148‑1 (MQ=255)
                                                                gTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAg                                                                                <  1:902839/148‑1 (MQ=255)
                                                                 tCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGa                                                                               >  1:322324/1‑148 (MQ=255)
                                                                  cAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGaa                                                                              <  2:1651650/148‑1 (MQ=255)
                                                                  cAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGaa                                                                              >  1:1538382/1‑148 (MQ=255)
                                                                     cGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGaaaaa                                                                           <  1:474606/148‑1 (MQ=255)
                                                                        tGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAaatc                                                                        <  1:106322/148‑1 (MQ=255)
                                                                          cGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAaatcaa                                                                      <  2:3212862/148‑1 (MQ=255)
                                                                            aaaaaaGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAaatcaatc                                                                    >  2:2955440/1‑148 (MQ=255)
                                                                              aaaaGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCg                                                                  >  1:2860084/1‑148 (MQ=255)
                                                                               aaaGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCgg                                                                 <  2:478126/148‑1 (MQ=255)
                                                                                        aTTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCAc                                                        >  2:859254/1‑148 (MQ=255)
                                                                                          tGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCg                                                      <  2:579650/148‑1 (MQ=255)
                                                                                            aCGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGAc                                                    >  1:2231045/1‑148 (MQ=255)
                                                                                            aCGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGAc                                                    >  1:100626/1‑148 (MQ=255)
                                                                                            aCGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGAc                                                    >  1:2231021/1‑148 (MQ=255)
                                                                                                cTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTAt                                                >  2:3057895/1‑148 (MQ=255)
                                                                                                cTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTAt                                                >  2:1567542/1‑148 (MQ=255)
                                                                                                  tCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGt                                              <  2:1374612/148‑1 (MQ=255)
                                                                                                  tCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGt                                              >  2:2386844/1‑148 (MQ=255)
                                                                                                   ccGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGtt                                             <  2:3257560/148‑1 (MQ=255)
                                                                                                    cGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTc                                            <  1:253406/148‑1 (MQ=255)
                                                                                                    cGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTc                                            <  1:186177/148‑1 (MQ=255)
                                                                                                      caaaggcaaagTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCg                                          >  1:1854496/1‑148 (MQ=255)
                                                                                                         aggcaaagTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGc                                       >  2:2823813/1‑148 (MQ=255)
                                                                                                            caaagTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGaa                                    <  2:1819613/148‑1 (MQ=255)
                                                                                                            caaagTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGaa                                    <  2:288380/148‑1 (MQ=255)
                                                                                                             aaagTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAAt                                   <  2:433057/148‑1 (MQ=255)
                                                                                                              aagTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATa                                  >  2:248740/1‑148 (MQ=255)
                                                                                                                gTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACa                                <  1:1593328/148‑1 (MQ=255)
                                                                                                                gTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACa                                <  1:2235667/148‑1 (MQ=255)
                                                                                                                 tGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACaa                               <  1:870710/148‑1 (MQ=255)
                                                                                                                  gCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAg                              >  2:1207291/1‑148 (MQ=255)
                                                                                                                     aaTGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTg                           <  1:657478/148‑1 (MQ=255)
                                                                                                                     aaTGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTg                           <  1:1913487/148‑1 (MQ=255)
                                                                                                                     aaTGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTg                           <  1:1928326/148‑1 (MQ=255)
                                                                                                                        gAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGt                        >  1:24252/1‑148 (MQ=255)
                                                                                                                        gAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGt                        >  2:1414671/1‑148 (MQ=255)
                                                                                                                                 tGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTc               <  1:3165674/148‑1 (MQ=255)
                                                                                                                                 tGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTc               <  1:3181599/148‑1 (MQ=255)
                                                                                                                                 tGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTc               >  1:2125450/1‑148 (MQ=255)
                                                                                                                                     cAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACtt           >  2:2071606/1‑148 (MQ=255)
                                                                                                                                        cGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTAct        <  1:3188228/148‑1 (MQ=255)
                                                                                                                                        cGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTAct        >  1:1767363/1‑148 (MQ=255)
                                                                                                                                         gTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTActc       <  1:567121/148‑1 (MQ=255)
                                                                                                                                          ttATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTActct      >  1:1280056/1‑148 (MQ=255)
                                                                                                                                           tATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTg     >  2:331258/1‑148 (MQ=255)
                                                                                                                                           tATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTg     >  2:1134097/1‑148 (MQ=255)
                                                                                                                                            aTGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTGt    <  1:3061647/148‑1 (MQ=255)
                                                                                                                                              ggCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTGTag  <  1:1220358/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTGTAG  >  CP009273/450621‑450910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTGTAGAGTTT  >  CP009273/450629‑450915
                                                                                                                                         |                                                                                                                                                     
GTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCC                                                                                                                                           >  SRR19973070.1502983/1‑150 (MQ=60)
GTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCC                                                                                                                                           >  SRR19973070.1502740/1‑150 (MQ=60)
  GTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAG                                                                                                                                         >  SRR19973070.1596591/1‑150 (MQ=60)
   TAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGG                                                                                                                                        <  SRR19973070.345303/150‑1 (MQ=60)
      CGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACG                                                                                                                                     <  SRR19973070.489524/150‑1 (MQ=60)
      CGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACG                                                                                                                                     <  SRR19973070.490034/150‑1 (MQ=60)
      CGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACG                                                                                                                                     <  SRR19973070.489442/150‑1 (MQ=60)
      CGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACG                                                                                                                                     <  SRR19973070.907257/150‑1 (MQ=60)
       GATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGT                                                                                                                                    >  SRR19973070.660780/1‑150 (MQ=60)
       GATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGT                                                                                                                                    >  SRR19973070.2325354/1‑150 (MQ=60)
              ATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGT                                                                                                                             >  SRR19973070.2904683/1‑150 (MQ=60)
              ATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGT                                                                                                                             >  SRR19973070.1244833/1‑150 (MQ=60)
                     CTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCTGA                                                                                                                      >  SRR19973070.1423756/1‑150 (MQ=60)
                     CTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCTGA                                                                                                                      >  SRR19973070.1423758/1‑150 (MQ=60)
                     CTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCTGA                                                                                                                      >  SRR19973070.1426663/1‑150 (MQ=60)
                     CTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCTGA                                                                                                                      >  SRR19973070.3258188/1‑150 (MQ=60)
                     CTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCTGA                                                                                                                      >  SRR19973070.3258182/1‑150 (MQ=60)
                      TGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGAT                                                                                                                     >  SRR19973070.290321/1‑150 (MQ=60)
                           GCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCC                                                                                                                <  SRR19973070.1308950/150‑1 (MQ=60)
                           GCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCC                                                                                                                >  SRR19973070.974137/1‑150 (MQ=60)
                               CGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAA                                                                                                            <  SRR19973070.674604/150‑1 (MQ=60)
                                GAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAAC                                                                                                           >  SRR19973070.3131909/1‑150 (MQ=60)
                                GAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAAC                                                                                                           <  SRR19973070.421370/150‑1 (MQ=60)
                                     CGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCAT                                                                                                      <  SRR19973070.2877402/150‑1 (MQ=60)
                                     CGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCAT                                                                                                      <  SRR19973070.2877346/150‑1 (MQ=60)
                                       CTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTG                                                                                                    >  SRR19973070.1239007/1‑150 (MQ=60)
                                          TTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACG                                                                                                 <  SRR19973070.1237154/150‑1 (MQ=60)
                                           TAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGC                                                                                                >  SRR19973070.249823/1‑150 (MQ=60)
                                           TAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGC                                                                                                >  SRR19973070.250083/1‑150 (MQ=60)
                                             AAAGCTAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCA                                                                                              <  SRR19973070.2997656/150‑1 (MQ=60)
                                                  GAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATT                                                                                         <  SRR19973070.981165/150‑1 (MQ=60)
                                                  GAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATT                                                                                         <  SRR19973070.981075/150‑1 (MQ=60)
                                                   AGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTA                                                                                        <  SRR19973070.340533/150‑1 (MQ=60)
                                                   AGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTA                                                                                        <  SRR19973070.340556/150‑1 (MQ=60)
                                                       GGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGA                                                                                    <  SRR19973070.2040017/150‑1 (MQ=60)
                                                       GGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGA                                                                                    <  SRR19973070.2039976/150‑1 (MQ=60)
                                                       GGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGA                                                                                    >  SRR19973070.324668/1‑150 (MQ=60)
                                                       GGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGA                                                                                    >  SRR19973070.324632/1‑150 (MQ=60)
                                                       GGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCATCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGA                                                                                    <  SRR19973070.362589/150‑1 (MQ=60)
                                                        GTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAA                                                                                   <  SRR19973070.909244/150‑1 (MQ=60)
                                                        GTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAA                                                                                   >  SRR19973070.1549195/1‑150 (MQ=60)
                                                             CGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAAT                                                                              <  SRR19973070.477959/150‑1 (MQ=60)
                                                              GTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTAAGCGTTATGGCTCGTCTGTACTCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACTCCATCATTAAATAAAAAATC                                                                             >  SRR19973070.158773/1‑150 (MQ=60)
                                                              GTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAACTGCCAATGAATAACGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGCTGACCAGATCAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATC                                                                             >  SRR19973070.2131157/1‑150 (MQ=60)
                                                                TGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAA                                                                           <  SRR19973070.1806414/150‑1 (MQ=60)
                                                                TGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAA                                                                           <  SRR19973070.107321/150‑1 (MQ=60)
                                                                 GCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAAT                                                                          <  SRR19973070.3173898/150‑1 (MQ=60)
                                                                    AAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCG                                                                       >  SRR19973070.2313167/1‑150 (MQ=60)
                                                                    AAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCG                                                                       >  SRR19973070.2878619/1‑150 (MQ=60)
                                                                                  TGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGAC                                                         >  SRR19973070.2245744/1‑150 (MQ=60)
                                                                                  TGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGAC                                                         >  SRR19973070.101590/1‑150 (MQ=60)
                                                                                  TGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGAC                                                         >  SRR19973070.2245720/1‑150 (MQ=60)
                                                                                            CGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCG                                               <  SRR19973070.255127/150‑1 (MQ=60)
                                                                                            CGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCG                                               <  SRR19973070.187626/150‑1 (MQ=60)
                                                                                            CGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCG                                               >  SRR19973070.1867196/1‑150 (MQ=60)
                                                                                            CGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCG                                               <  SRR19973070.187599/150‑1 (MQ=60)
                                                                                               AAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGC                                            >  SRR19973070.978176/1‑150 (MQ=60)
                                                                                               AAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACGTATGTTCCGGGC                                            >  SRR19973070.2641784/1‑150 (MQ=60)
                                                                                                        GTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAG                                   <  SRR19973070.1604343/150‑1 (MQ=60)
                                                                                                        GTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAG                                   <  SRR19973070.1604467/150‑1 (MQ=60)
                                                                                                        GTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAG                                   <  SRR19973070.2250407/150‑1 (MQ=60)
                                                                                                        GTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAG                                   <  SRR19973070.2250389/150‑1 (MQ=60)
                                                                                                         TGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGC                                  <  SRR19973070.876803/150‑1 (MQ=60)
                                                                                                             AATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGG                              <  SRR19973070.1925948/150‑1 (MQ=60)
                                                                                                             AATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGG                              <  SRR19973070.1926412/150‑1 (MQ=60)
                                                                                                             AATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGG                              <  SRR19973070.1941319/150‑1 (MQ=60)
                                                                                                             AATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGG                              <  SRR19973070.662160/150‑1 (MQ=60)
                                                                                                              ATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGT                             >  SRR19973070.24592/1‑150 (MQ=60)
                                                                                                                    ATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGAC                       >  SRR19973070.3088933/1‑150 (MQ=60)
                                                                                                                    ATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGAC                       >  SRR19973070.989580/1‑150 (MQ=60)
                                                                                                                      CGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTT                     >  SRR19973070.448418/1‑150 (MQ=60)
                                                                                                                       GTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTC                    >  SRR19973070.2139670/1‑150 (MQ=60)
                                                                                                                         TGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCAC                  <  SRR19973070.968640/150‑1 (MQ=60)
                                                                                                                         TGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCAC                  <  SRR19973070.3202188/150‑1 (MQ=60)
                                                                                                                         TGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCAC                  <  SRR19973070.3186158/150‑1 (MQ=60)
                                                                                                                          GCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACT                 >  SRR19973070.778697/1‑150 (MQ=60)
                                                                                                                              AGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACT             >  SRR19973070.1779592/1‑150 (MQ=60)
                                                                                                                              AGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACT             >  SRR19973070.1370979/1‑150 (MQ=60)
                                                                                                                                CGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCT           <  SRR19973070.3208855/150‑1 (MQ=60)
                                                                                                                                CGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCT           <  SRR19973070.3208835/150‑1 (MQ=60)
                                                                                                                                CGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCT           >  SRR19973070.1289001/1‑150 (MQ=60)
                                                                                                                                CGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCT           >  SRR19973070.1289003/1‑150 (MQ=60)
                                                                                                                                 GTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTG          <  SRR19973070.2209813/150‑1 (MQ=60)
                                                                                                                                 GTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTG          <  SRR19973070.570933/150‑1 (MQ=60)
                                                                                                                                 GTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTG          <  SRR19973070.570509/150‑1 (MQ=60)
                                                                                                                                   TATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTGTA        <  SRR19973070.2382020/150‑1 (MQ=60)
                                                                                                                                    ATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTGTAG       <  SRR19973070.3081248/150‑1 (MQ=60)
                                                                                                                                    ATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTGTAG       <  SRR19973070.3080439/150‑1 (MQ=60)
                                                                                                                                      GGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTGTAGAG     <  SRR19973070.1228934/150‑1 (MQ=60)
                                                                                                                                      GGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTGTAGAG     <  SRR19973070.1228909/150‑1 (MQ=60)
                                                                                                                                      GGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTGTAGAG     <  SRR19973070.1228908/150‑1 (MQ=60)
                                                                                                                                         TCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTGTAGAGTTT  <  SRR19973070.2444044/150‑1 (MQ=60)
                                                                                                                                         |                                                                                                                                                     
GTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTGTAGAGTTT  >  CP009273/450629‑450915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑