Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 450,766 G→T 100% A60S (GCG→TCG)  tig → peptidyl‑prolyl cis/trans isomerase (trigger factor)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273450,7660GT95.0% 589.4 / 20.5 199A60S (GCG→TCG) tigpeptidyl‑prolyl cis/trans isomerase (trigger factor)
Reads supporting (aligned to +/- strand):  ref base G (9/1);  new base T (77/112);  total (86/113)
Fisher's exact test for biased strand distribution p-value = 2.60e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 200 total aligned reads displayed.

TGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTGTAG  >  CP009273/450621‑450910
                                                                                                                                                 |                                                                                                                                                
   ccgccgTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCt                                                                                                                                             <  2:2683213/148‑1 (MQ=255)
   ccgccgTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCt                                                                                                                                             <  1:468049/148‑1 (MQ=255)
    cgccgTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTg                                                                                                                                            >  1:3062771/1‑148 (MQ=255)
         tgtAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGc                                                                                                                                       <  1:561974/148‑1 (MQ=255)
         tgtAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGc                                                                                                                                       <  1:2347443/148‑1 (MQ=255)
           tAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCa                                                                                                                                     <  2:959949/148‑1 (MQ=255)
           tAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCa                                                                                                                                     <  1:1041652/148‑1 (MQ=255)
              cGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGa                                                                                                                                  >  2:2041602/1‑148 (MQ=255)
               gATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGAc                                                                                                                                 >  1:2022558/1‑148 (MQ=255)
                aTTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACg                                                                                                                                >  2:346533/1‑148 (MQ=255)
                 ttACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGt                                                                                                                               >  2:2912052/1‑148 (MQ=255)
                  tACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGtt                                                                                                                              >  2:2553326/1‑148 (MQ=255)
                  tACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGtt                                                                                                                              >  1:2470526/1‑148 (MQ=255)
                         gctgctGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTg                                                                                                                       >  2:3478238/1‑148 (MQ=255)
                           tgctgACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGAc                                                                                                                     <  1:3287376/148‑1 (MQ=255)
                            gctgACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGAcc                                                                                                                    <  1:360787/148‑1 (MQ=255)
                             ctgACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGCTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCt                                                                                                                   >  1:2259445/1‑148 (MQ=255)
                              tgACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCtg                                                                                                                  <  1:1912755/148‑1 (MQ=255)
                               gACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCtga                                                                                                                 >  2:1637331/1‑148 (MQ=255)
                               gACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCtga                                                                                                                 >  2:1637220/1‑148 (MQ=255)
                                 cAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCtgatg                                                                                                               >  1:3217668/1‑148 (MQ=255)
                                  aGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCtgatga                                                                                                              <  2:3153628/148‑1 (MQ=255)
                                  aGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCtgatga                                                                                                              <  2:118583/148‑1 (MQ=255)
                                  aGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCtgatga                                                                                                              <  1:1651216/148‑1 (MQ=255)
                                     aTCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGcc                                                                                                           <  2:478070/148‑1 (MQ=255)
                                      tCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCg                                                                                                          >  1:1687158/1‑148 (MQ=255)
                                       cGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGt                                                                                                         >  2:1793793/1‑148 (MQ=255)
                                           aCCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAATTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACt                                                                                                     <  1:767440/148‑1 (MQ=255)
                                            ccGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACtt                                                                                                    >  2:2889724/1‑148 (MQ=255)
                                              gCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCa                                                                                                  <  2:3378224/148‑1 (MQ=255)
                                               cTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCAt                                                                                                 <  1:1969802/148‑1 (MQ=255)
                                                tGTTAATAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCACAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCAtt                                                                                                <  1:3259713/148‑1 (MQ=255)
                                                tGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCAtt                                                                                                <  2:3233031/148‑1 (MQ=255)
                                                 gTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGATCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTg                                                                                               >  2:3359165/1‑148 (MQ=255)
                                                        gCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCcat                                                                                        <  1:2278627/148‑1 (MQ=255)
                                                        gCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGAcat                                                                                        >  1:301864/1‑148 (MQ=255)
                                                           aGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCcatcat                                                                                     >  2:1604379/1‑148 (MQ=255)
                                                                   aaCGTTGCGAAAAAAGTACGTAGTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGaaa                                                                             <  2:2856378/148‑1 (MQ=255)
                                                                     cGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGaaaaa                                                                           >  1:1997953/1‑148 (MQ=255)
                                                                       ttGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAaat                                                                         <  2:3074321/148‑1 (MQ=255)
                                                                       ttGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAaat                                                                         <  2:1304474/148‑1 (MQ=255)
                                                                       ttGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAaat                                                                         >  2:657563/1‑148 (MQ=255)
                                                                        tGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAaatc                                                                        <  2:3431350/148‑1 (MQ=255)
                                                                           gAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAaatcaat                                                                     <  1:1638409/148‑1 (MQ=255)
                                                                           gAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAaatcaat                                                                     <  1:2801665/148‑1 (MQ=255)
                                                                            aaaaaaGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAaatcaatc                                                                    <  1:3478238/148‑1 (MQ=255)
                                                                            aaaaaaGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAaatcaatc                                                                    <  1:500178/148‑1 (MQ=255)
                                                                              aaaaGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCg                                                                  >  2:1800942/1‑148 (MQ=255)
                                                                               aaaGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCgg                                                                 <  2:854008/148‑1 (MQ=255)
                                                                                aaGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGc                                                                <  1:1011644/148‑1 (MQ=255)
                                                                                 aGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCt                                                               >  2:3553577/1‑148 (MQ=255)
                                                                                   tACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTgg                                                             >  1:2158444/1‑148 (MQ=255)
                                                                                        aTTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCAc                                                        <  1:3046624/148‑1 (MQ=255)
                                                                                          tGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCg                                                      <  1:1174992/148‑1 (MQ=255)
                                                                                           gACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGa                                                     <  2:2054742/148‑1 (MQ=255)
                                                                                           gACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGa                                                     <  1:1494381/148‑1 (MQ=255)
                                                                                           gACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGa                                                     >  1:2361630/1‑148 (MQ=255)
                                                                                            aCGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGAc                                                    <  1:3402427/148‑1 (MQ=255)
                                                                                            aCGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGAc                                                    >  1:2350218/1‑148 (MQ=255)
                                                                                               gCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTa                                                 <  2:748491/148‑1 (MQ=255)
                                                                                                 ttCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATg                                               >  1:140894/1‑148 (MQ=255)
                                                                                                  tCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGt                                              >  2:2553992/1‑148 (MQ=255)
                                                                                                         aggcaaagTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGc                                       >  1:2329119/1‑148 (MQ=255)
                                                                                                         aggcaaagTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGc                                       >  2:2271792/1‑148 (MQ=255)
                                                                                                          ggcaaagTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCg                                      <  1:892327/148‑1 (MQ=255)
                                                                                                            caaagTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGaa                                    <  1:1815359/148‑1 (MQ=255)
                                                                                                            caaagTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGaa                                    <  2:954480/148‑1 (MQ=255)
                                                                                                             aaagTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAAt                                   <  1:2455101/148‑1 (MQ=255)
                                                                                                              aagTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATa                                  <  1:3494443/148‑1 (MQ=255)
                                                                                                               agTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATAc                                 <  1:933379/148‑1 (MQ=255)
                                                                                                               agTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATAc                                 >  2:50934/1‑148 (MQ=255)
                                                                                                                  gCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAg                              <  1:1637316/148‑1 (MQ=255)
                                                                                                                   ccAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGc                             <  2:2022558/148‑1 (MQ=255)
                                                                                                                    cAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCt                            <  1:461683/148‑1 (MQ=255)
                                                                                                                    cAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCt                            <  1:649795/148‑1 (MQ=255)
                                                                                                                     aaTGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTg                           >  2:1469686/1‑148 (MQ=255)
                                                                                                                         aaTATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTg                       >  1:1745923/1‑148 (MQ=255)
                                                                                                                          atatCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGa                      <  2:1667509/148‑1 (MQ=255)
                                                                                                                          atatCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGa                      >  1:1072660/1‑148 (MQ=255)
                                                                                                                             tCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGa                   >  2:3500027/1‑148 (MQ=255)
                                                                                                                              cGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGAc                  <  1:2732016/148‑1 (MQ=255)
                                                                                                                              cGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGAc                  >  1:3418883/1‑148 (MQ=255)
                                                                                                                              cGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGAc                  <  1:2317602/148‑1 (MQ=255)
                                                                                                                                ttGCTCTGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACGTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTTGCGCACCGACTTATGTTCCGGGCGAATACCAGCTGGGTGAAGACtt                <  2:2950852/148‑1 (MQ=255)
                                                                                                                                ttGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACtt                >  1:2063487/1‑148 (MQ=255)
                                                                                                                                 tGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTc               <  1:208988/148‑1 (MQ=255)
                                                                                                                                  gCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCa              >  1:131620/1‑148 (MQ=255)
                                                                                                                                   cTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCAc             <  1:1582289/148‑1 (MQ=255)
                                                                                                                                   cTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCAc             >  2:866747/1‑148 (MQ=255)
                                                                                                                                    tCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACt            <  1:2768524/148‑1 (MQ=255)
                                                                                                                                    tCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACt            <  1:1399348/148‑1 (MQ=255)
                                                                                                                                     cAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACtt           <  2:2896772/148‑1 (MQ=255)
                                                                                                                                      aGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTa          <  1:3460342/148‑1 (MQ=255)
                                                                                                                                       gCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTAc         >  2:232743/1‑148 (MQ=255)
                                                                                                                                        cGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTAct        >  2:1969083/1‑148 (MQ=255)
                                                                                                                                           tATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTg     <  1:1768820/148‑1 (MQ=255)
                                                                                                                                           tATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTg     <  2:2262714/148‑1 (MQ=255)
                                                                                                                                              ggCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTGTag  >  2:3050482/1‑148 (MQ=255)
                                                                                                                                              ggCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTGTag  <  2:2748815/148‑1 (MQ=255)
                                                                                                                                              ggCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTGTag  <  2:1318037/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTGTAG  >  CP009273/450621‑450910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTGTAGAGTT  >  CP009273/450619‑450914
                                                                                                                                                   |                                                                                                                                                    
CTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCG                                                                                                                                                    >  SRR19973067.2068446/1‑150 (MQ=60)
    GCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTG                                                                                                                                                >  SRR19973067.3082267/1‑150 (MQ=60)
     CCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGT                                                                                                                                               <  SRR19973067.471391/150‑1 (MQ=60)
     CCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGT                                                                                                                                               <  SRR19973067.471287/150‑1 (MQ=60)
     CCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGT                                                                                                                                               <  SRR19973067.471230/150‑1 (MQ=60)
           TGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCA                                                                                                                                         <  SRR19973067.565643/150‑1 (MQ=60)
           TGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCA                                                                                                                                         <  SRR19973067.565462/150‑1 (MQ=60)
           TGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCA                                                                                                                                         <  SRR19973067.2362779/150‑1 (MQ=60)
             TAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGG                                                                                                                                       <  SRR19973067.1048718/150‑1 (MQ=60)
             TAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGG                                                                                                                                       <  SRR19973067.1048813/150‑1 (MQ=60)
               ACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGAC                                                                                                                                     >  SRR19973067.2035970/1‑150 (MQ=60)
               ACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGAC                                                                                                                                     >  SRR19973067.2036138/1‑150 (MQ=60)
                  ATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTT                                                                                                                                  >  SRR19973067.2486766/1‑150 (MQ=60)
                            CTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACC                                                                                                                        <  SRR19973067.1781554/150‑1 (MQ=60)
                             TGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCT                                                                                                                       <  SRR19973067.3308024/150‑1 (MQ=60)
                             TGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCT                                                                                                                       >  SRR19973067.2274150/1‑150 (MQ=60)
                             TGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGCTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCT                                                                                                                       >  SRR19973067.2274435/1‑150 (MQ=60)
                              GCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTG                                                                                                                      <  SRR19973067.363288/150‑1 (MQ=60)
                                TGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGAT                                                                                                                    <  SRR19973067.1925807/150‑1 (MQ=60)
                                 GACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATG                                                                                                                   >  SRR19973067.3237910/1‑150 (MQ=60)
                                 GACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATG                                                                                                                   >  SRR19973067.3240692/1‑150 (MQ=60)
                                   CAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAG                                                                                                                 <  SRR19973067.214157/150‑1 (MQ=60)
                                    AGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGC                                                                                                                <  SRR19973067.1662583/150‑1 (MQ=60)
                                    AGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGC                                                                                                                <  SRR19973067.1662588/150‑1 (MQ=60)
                                      CATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCG                                                                                                              >  SRR19973067.1698779/1‑150 (MQ=60)
                                          GAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAAC                                                                                                          >  SRR19973067.1410669/1‑150 (MQ=60)
                                             ACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAATTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTC                                                                                                       <  SRR19973067.772768/150‑1 (MQ=60)
                                                 CTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTG                                                                                                   <  SRR19973067.3575450/150‑1 (MQ=60)
                                                 CTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTG                                                                                                   >  SRR19973067.1714737/1‑150 (MQ=60)
                                                 CTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTG                                                                                                   <  SRR19973067.1983160/150‑1 (MQ=60)
                                                  TGTTAATAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCACAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGA                                                                                                  <  SRR19973067.3280077/150‑1 (MQ=60)
                                                       AAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCA                                                                                             <  SRR19973067.1770388/150‑1 (MQ=60)
                                                        AAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGACAT                                                                                            >  SRR19973067.303796/1‑150 (MQ=60)
                                                          GCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCA                                                                                          <  SRR19973067.2293697/150‑1 (MQ=60)
                                                                  GTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAA                                                                                  <  SRR19973067.1407970/150‑1 (MQ=60)
                                                                    CAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAA                                                                                >  SRR19973067.414207/1‑150 (MQ=60)
                                                                     AACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAA                                                                               >  SRR19973067.2011440/1‑150 (MQ=60)
                                                                        GTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGAACTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATC                                                                            <  SRR19973067.3469209/150‑1 (MQ=60)
                                                                            CGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATC                                                                        >  SRR19973067.195419/1‑150 (MQ=60)
                                                                            CGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATC                                                                        <  SRR19973067.3313598/150‑1 (MQ=60)
                                                                             GAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCC                                                                       >  SRR19973067.1486854/1‑150 (MQ=60)
                                                                             GAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCC                                                                       <  SRR19973067.1649683/150‑1 (MQ=60)
                                                                             GAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCC                                                                       <  SRR19973067.2819735/150‑1 (MQ=60)
                                                                              AAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCG                                                                      <  SRR19973067.3500318/150‑1 (MQ=60)
                                                                              AAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCG                                                                      <  SRR19973067.503614/150‑1 (MQ=60)
                                                                              AAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCG                                                                      >  SRR19973067.2766581/1‑150 (MQ=60)
                                                                               AAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGG                                                                     <  SRR19973067.765117/150‑1 (MQ=60)
                                                                                AAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGC                                                                    >  SRR19973067.3045318/1‑150 (MQ=60)
                                                                                  AAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTG                                                                  <  SRR19973067.1057980/150‑1 (MQ=60)
                                                                                  AAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTG                                                                  <  SRR19973067.1018592/150‑1 (MQ=60)
                                                                                  AAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTAGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTG                                                                  <  SRR19973067.2305954/150‑1 (MQ=60)
                                                                                   AGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGG                                                                 >  SRR19973067.2172878/1‑150 (MQ=60)
                                                                                    GTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGC                                                                >  SRR19973067.632499/1‑150 (MQ=60)
                                                                                    GTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGC                                                                >  SRR19973067.2428927/1‑150 (MQ=60)
                                                                                          ATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCG                                                          <  SRR19973067.3066032/150‑1 (MQ=60)
                                                                                          ATTGACGGCGTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCG                                                          <  SRR19973067.3066324/150‑1 (MQ=60)
                                                                                           TTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGA                                                         >  SRR19973067.2377019/1‑150 (MQ=60)
                                                                                            TGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGAC                                                        >  SRR19973067.1679011/1‑150 (MQ=60)
                                                                                            TGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGAC                                                        >  SRR19973067.2365522/1‑150 (MQ=60)
                                                                                            TGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGAC                                                        >  SRR19973067.2365564/1‑150 (MQ=60)
                                                                                            TGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGAC                                                        <  SRR19973067.1182769/150‑1 (MQ=60)
                                                                                             GACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACT                                                       <  SRR19973067.1504786/150‑1 (MQ=60)
                                                                                             GACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACT                                                       <  SRR19973067.1504245/150‑1 (MQ=60)
                                                                                              ACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTT                                                      <  SRR19973067.3424085/150‑1 (MQ=60)
                                                                                              ACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTT                                                      <  SRR19973067.3424092/150‑1 (MQ=60)
                                                                                                 GCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATG                                                   >  SRR19973067.1873915/1‑150 (MQ=60)
                                                                                                 GCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATG                                                   >  SRR19973067.142139/1‑150 (MQ=60)
                                                                                                  CTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGT                                                  <  SRR19973067.2009172/150‑1 (MQ=60)
                                                                                                         AAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGC                                           >  SRR19973067.2344417/1‑150 (MQ=60)
                                                                                                         AAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGC                                           >  SRR19973067.2344405/1‑150 (MQ=60)
                                                                                                         AAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGC                                           >  SRR19973067.2344282/1‑150 (MQ=60)
                                                                                                         AAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGC                                           >  SRR19973067.2972518/1‑150 (MQ=60)
                                                                                                            GGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAA                                        <  SRR19973067.898229/150‑1 (MQ=60)
                                                                                                              CAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATA                                      <  SRR19973067.1827648/150‑1 (MQ=60)
                                                                                                               AAAGTTCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATAC                                     >  SRR19973067.2572000/1‑150 (MQ=60)
                                                                                                               AAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATAC                                     <  SRR19973067.2471266/150‑1 (MQ=60)
                                                                                                               AAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATAC                                     <  SRR19973067.2471243/150‑1 (MQ=60)
                                                                                                               AAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATAC                                     >  SRR19973067.2447600/1‑150 (MQ=60)
                                                                                                                AAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACA                                    <  SRR19973067.3516613/150‑1 (MQ=60)
                                                                                                                 AGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAA                                   <  SRR19973067.348927/150‑1 (MQ=60)
                                                                                                                 AGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAA                                   <  SRR19973067.939700/150‑1 (MQ=60)
                                                                                                                 AGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAA                                   <  SRR19973067.939711/150‑1 (MQ=60)
                                                                                                                    GCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCT                                <  SRR19973067.1648486/150‑1 (MQ=60)
                                                                                                                    GCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCT                                <  SRR19973067.1648583/150‑1 (MQ=60)
                                                                                                                    GCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCT                                <  SRR19973067.1648598/150‑1 (MQ=60)
                                                                                                                      CAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGG                              <  SRR19973067.2286836/150‑1 (MQ=60)
                                                                                                                      CAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGG                              <  SRR19973067.654202/150‑1 (MQ=60)
                                                                                                                      CAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGG                              <  SRR19973067.464878/150‑1 (MQ=60)
                                                                                                                         TGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTG                           >  SRR19973067.1757839/1‑150 (MQ=60)
                                                                                                                          GAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGA                          >  SRR19973067.1080016/1‑150 (MQ=60)
                                                                                                                          GAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGA                          <  SRR19973067.788983/150‑1 (MQ=60)
                                                                                                                          GAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGA                          >  SRR19973067.1081010/1‑150 (MQ=60)
                                                                                                                              ATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGAC                      >  SRR19973067.3440641/1‑150 (MQ=60)
                                                                                                                                CGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTT                    <  SRR19973067.2749647/150‑1 (MQ=60)
                                                                                                                                CGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTT                    <  SRR19973067.1215398/150‑1 (MQ=60)
                                                                                                                                CGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTT                    <  SRR19973067.2332844/150‑1 (MQ=60)
                                                                                                                                CGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTT                    >  SRR19973067.2077219/1‑150 (MQ=60)
                                                                                                                                  TTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCA                  >  SRR19973067.132809/1‑150 (MQ=60)
                                                                                                                                   TGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCAC                 <  SRR19973067.210025/150‑1 (MQ=60)
                                                                                                                                   TGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCAC                 <  SRR19973067.210552/150‑1 (MQ=60)
                                                                                                                                     CTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTT               <  SRR19973067.1593124/150‑1 (MQ=60)
                                                                                                                                      TCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTA              <  SRR19973067.1408845/150‑1 (MQ=60)
                                                                                                                                      TCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTA              <  SRR19973067.2786413/150‑1 (MQ=60)
                                                                                                                                      TCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTA              <  SRR19973067.2786416/150‑1 (MQ=60)
                                                                                                                                       CAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTAC             >  SRR19973067.3505292/1‑150 (MQ=60)
                                                                                                                                        AGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACT            <  SRR19973067.3482340/150‑1 (MQ=60)
                                                                                                                                           GTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTG         <  SRR19973067.3098362/150‑1 (MQ=60)
                                                                                                                                             TATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTGTA       <  SRR19973067.1780834/150‑1 (MQ=60)
                                                                                                                                             TATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTGTA       <  SRR19973067.3296105/150‑1 (MQ=60)
                                                                                                                                                 GCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTGTAGAGT   >  SRR19973067.2235081/1‑150 (MQ=60)
                                                                                                                                                  CTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTGTAGAGTT  <  SRR19973067.2055257/150‑1 (MQ=60)
                                                                                                                                                  CTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTGTAGAGTT  <  SRR19973067.2053709/150‑1 (MQ=60)
                                                                                                                                                  CTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTGTAGAGTT  <  SRR19973067.634295/150‑1 (MQ=60)
                                                                                                                                                  CTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTGTAGAGTT  <  SRR19973067.3485535/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                    
CTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTGTAGAGTT  >  CP009273/450619‑450914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑