Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 450,766 G→T A60S (GCG→TCG)  tig → peptidyl‑prolyl cis/trans isomerase (trigger factor)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273450,7660GT99.5% 634.3 / NA 191A60S (GCG→TCG) tigpeptidyl‑prolyl cis/trans isomerase (trigger factor)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (83/107);  minor base A (1/0);  total (84/107)
Fisher's exact test for biased strand distribution p-value = 4.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 192 total aligned reads displayed.

CTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTGTAG  >  CP009273/450619‑450910
                                                                                                                                                   |                                                                                                                                                
cTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGctcg                                                                                                                                                <  1:1004728/150‑1 (MQ=255)
   ggCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCt                                                                                                                                             <  2:1274356/150‑1 (MQ=255)
     ccgccgTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGt                                                                                                                                           <  2:111000/150‑1 (MQ=255)
       gccgTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTAc                                                                                                                                         <  1:2832595/150‑1 (MQ=255)
        ccgTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACg                                                                                                                                        <  1:968911/150‑1 (MQ=255)
           tgtAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCa                                                                                                                                     <  1:565737/150‑1 (MQ=255)
             tAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAgg                                                                                                                                   >  2:379490/1‑150 (MQ=255)
             tAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAgg                                                                                                                                   >  2:2287190/1‑150 (MQ=255)
               aCGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGAc                                                                                                                                 <  2:1807562/150‑1 (MQ=255)
                  aTTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGtt                                                                                                                              >  2:896099/1‑150 (MQ=255)
                  aTTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGtt                                                                                                                              <  2:3258589/150‑1 (MQ=255)
                  aTTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGtt                                                                                                                              >  2:2856573/1‑150 (MQ=255)
                   ttACTATCGCTGCTGACAGCTTCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTc                                                                                                                             <  2:3407528/150‑1 (MQ=255)
                    tACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCt                                                                                                                            >  2:1650280/1‑150 (MQ=255)
                    tACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCt                                                                                                                            <  1:458656/150‑1 (MQ=255)
                      cTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTgg                                                                                                                          <  1:3767641/150‑1 (MQ=255)
                       tATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTggg                                                                                                                         <  2:758480/150‑1 (MQ=255)
                        aTCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGt                                                                                                                        >  1:2280735/1‑150 (MQ=255)
                          cGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGa                                                                                                                      <  2:3672322/150‑1 (MQ=255)
                           gctgctGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGAc                                                                                                                     <  2:2011303/150‑1 (MQ=255)
                              gctgACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCtg                                                                                                                  >  1:2042573/1‑150 (MQ=255)
                               ctgACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCtga                                                                                                                 <  1:1593775/150‑1 (MQ=255)
                               ctgACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCtga                                                                                                                 <  2:3193288/150‑1 (MQ=255)
                                tgACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCtgat                                                                                                                >  1:2239195/1‑150 (MQ=255)
                                tgACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCtgat                                                                                                                >  2:2595415/1‑150 (MQ=255)
                                tgACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAAAGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCtgat                                                                                                                <  1:734253/150‑1 (MQ=255)
                                 gACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCtgatg                                                                                                               >  2:1062574/1‑150 (MQ=255)
                                   cAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAg                                                                                                             <  2:3182937/150‑1 (MQ=255)
                                     gCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGcc                                                                                                           <  1:3347671/150‑1 (MQ=255)
                                      cATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCg                                                                                                          >  1:1013803/1‑150 (MQ=255)
                                      cATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCg                                                                                                          >  2:2323114/1‑150 (MQ=255)
                                       aTCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGt                                                                                                         >  1:3579751/1‑150 (MQ=255)
                                        tCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTa                                                                                                        <  2:326079/150‑1 (MQ=255)
                                        tCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTa                                                                                                        <  2:113509/150‑1 (MQ=255)
                                        tCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTa                                                                                                        <  2:2382345/150‑1 (MQ=255)
                                          gagaCCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAAc                                                                                                      <  2:2866971/150‑1 (MQ=255)
                                               cGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCAt                                                                                                 <  2:1346999/150‑1 (MQ=255)
                                               cGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCAt                                                                                                 >  1:2191099/1‑150 (MQ=255)
                                                  tGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGa                                                                                              >  2:3355129/1‑150 (MQ=255)
                                                  tGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGa                                                                                              >  1:1826619/1‑150 (MQ=255)
                                                  tGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGa                                                                                              <  2:2278278/150‑1 (MQ=255)
                                                   gTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGAc                                                                                             >  1:1036115/1‑150 (MQ=255)
                                                   gTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGAc                                                                                             <  1:2356712/150‑1 (MQ=255)
                                                   gTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGAc                                                                                             <  2:2173549/150‑1 (MQ=255)
                                                    ttAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACg                                                                                            >  2:2519336/1‑150 (MQ=255)
                                                    ttAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACg                                                                                            >  2:1792592/1‑150 (MQ=255)
                                                          gCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCcatca                                                                                      <  2:3149391/150‑1 (MQ=255)
                                                          gCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCcatca                                                                                      <  1:3434968/150‑1 (MQ=255)
                                                           cGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCcatcat                                                                                     <  1:3761689/150‑1 (MQ=255)
                                                           cGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCcatcat                                                                                     <  1:1115809/150‑1 (MQ=255)
                                                            gAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCAtt                                                                                    >  1:1758017/1‑150 (MQ=255)
                                                             aGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTa                                                                                   <  2:670967/150‑1 (MQ=255)
                                                               cTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTaaa                                                                                 >  1:2139017/1‑150 (MQ=255)
                                                                 ggTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGa                                                                               <  2:2239195/150‑1 (MQ=255)
                                                                       cGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAaat                                                                         <  1:1650280/150‑1 (MQ=255)
                                                                          tGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAaatcaa                                                                      >  1:2389402/1‑150 (MQ=255)
                                                                          tGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAaatcaa                                                                      <  2:832565/150‑1 (MQ=255)
                                                                          tGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAaatcaa                                                                      <  1:2088182/150‑1 (MQ=255)
                                                                            cGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAaatcaatc                                                                    <  1:993529/150‑1 (MQ=255)
                                                                            cGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAaatcaatc                                                                    <  1:3972/150‑1 (MQ=255)
                                                                             gAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATcc                                                                   >  2:1900706/1‑150 (MQ=255)
                                                                              aaaaaaGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCg                                                                  >  2:3117906/1‑150 (MQ=255)
                                                                                 aaaGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCt                                                               >  2:1028129/1‑150 (MQ=255)
                                                                                  aaGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTg                                                              <  1:542403/150‑1 (MQ=255)
                                                                                       cGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCa                                                         >  1:3114199/1‑150 (MQ=255)
                                                                                       cGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCa                                                         >  2:3703787/1‑150 (MQ=255)
                                                                                        gTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTAGCTCAGCGTTATGGCTCGTCTGTACTCCAGTACTTAATTTGAGACATGATGAACCGTAACTTCATTGACTCAATCATAAAAGAAAAAATCAATCCGTCTGGCGCAc                                                        >  1:3718978/1‑150 (MQ=255)
                                                                                          aTTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCg                                                      <  2:118587/150‑1 (MQ=255)
                                                                                          aTTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCg                                                      >  1:3517829/1‑150 (MQ=255)
                                                                                           ttGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGa                                                     >  1:2497973/1‑150 (MQ=255)
                                                                                                ggCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTAt                                                <  1:3453045/150‑1 (MQ=255)
                                                                                                ggCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTAt                                                <  1:1749543/150‑1 (MQ=255)
                                                                                                  cTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGt                                              >  2:1297488/1‑150 (MQ=255)
                                                                                                    tCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTc                                            <  2:1753076/150‑1 (MQ=255)
                                                                                                    tCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTc                                            <  1:3161160/150‑1 (MQ=255)
                                                                                                     ccGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTcc                                           <  1:2331493/150‑1 (MQ=255)
                                                                                                     ccGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTcc                                           <  1:2696081/150‑1 (MQ=255)
                                                                                                      cGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCg                                          <  2:2639643/150‑1 (MQ=255)
                                                                                                      cGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCg                                          >  1:2765262/1‑150 (MQ=255)
                                                                                                        caaaggcaaagTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCggg                                        >  1:3547918/1‑150 (MQ=255)
                                                                                                        caaaggcaaagTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCggg                                        >  1:2508053/1‑150 (MQ=255)
                                                                                                         aaaggcaaagTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGc                                       <  1:1993685/150‑1 (MQ=255)
                                                                                                            ggcaaagTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGaa                                    >  1:488261/1‑150 (MQ=255)
                                                                                                              caaagTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATa                                  <  1:2621648/150‑1 (MQ=255)
                                                                                                               aaagTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATAc                                 <  2:3579751/150‑1 (MQ=255)
                                                                                                               aaagTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATAc                                 >  2:3314049/1‑150 (MQ=255)
                                                                                                                 agTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACaa                               >  2:1534993/1‑150 (MQ=255)
                                                                                                                 agTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACaa                               >  1:126508/1‑150 (MQ=255)
                                                                                                                  gTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAg                              <  2:2687629/150‑1 (MQ=255)
                                                                                                                     ccAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTg                           <  1:2851500/150‑1 (MQ=255)
                                                                                                                     ccAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTg                           <  2:3067019/150‑1 (MQ=255)
                                                                                                                           aaTATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGaa                     >  1:1508291/1‑150 (MQ=255)
                                                                                                                            atatCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAg                    >  1:1916599/1‑150 (MQ=255)
                                                                                                                            atatCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAg                    >  1:2443288/1‑150 (MQ=255)
                                                                                                                            atatCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAg                    >  1:31821/1‑150 (MQ=255)
                                                                                                                            atatCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAg                    >  1:1428666/1‑150 (MQ=255)
                                                                                                                                cGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACtt                <  1:3238569/150‑1 (MQ=255)
                                                                                                                                cGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACtt                <  2:1521421/150‑1 (MQ=255)
                                                                                                                                   tGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTAATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCAc             <  1:1900706/150‑1 (MQ=255)
                                                                                                                                      tCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTa          <  1:379490/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTGTAG  >  CP009273/450619‑450910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTGTAGAGT  >  CP009273/450617‑450913
                                                                                                                                                     |                                                                                                                                                   
GCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCT                                                                                                                                                     <  SRR19973061.1602380/150‑1 (MQ=60)
  CTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCG                                                                                                                                                   <  SRR19973061.1007287/150‑1 (MQ=60)
   TTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGT                                                                                                                                                  >  SRR19973061.2947852/1‑150 (MQ=60)
    TGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTC                                                                                                                                                 >  SRR19973061.3535869/1‑150 (MQ=60)
       CCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGT                                                                                                                                              <  SRR19973061.1797731/150‑1 (MQ=60)
        CGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTA                                                                                                                                             >  SRR19973061.3787216/1‑150 (MQ=60)
         GCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTAC                                                                                                                                            <  SRR19973061.2839850/150‑1 (MQ=60)
         GCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTAC                                                                                                                                            <  SRR19973061.386569/150‑1 (MQ=60)
          CCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACG                                                                                                                                           <  SRR19973061.971364/150‑1 (MQ=60)
             TGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCA                                                                                                                                        <  SRR19973061.567218/150‑1 (MQ=60)
              GTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAG                                                                                                                                       >  SRR19973061.1591428/1‑150 (MQ=60)
                     TTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTC                                                                                                                                >  SRR19973061.2048550/1‑150 (MQ=60)
                      TACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCT                                                                                                                               <  SRR19973061.459901/150‑1 (MQ=60)
                        CTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGG                                                                                                                             <  SRR19973061.3777265/150‑1 (MQ=60)
                          ATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGT                                                                                                                           >  SRR19973061.2286497/1‑150 (MQ=60)
                                GCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTG                                                                                                                     >  SRR19973061.2047706/1‑150 (MQ=60)
                                GCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCACAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTG                                                                                                                     <  SRR19973061.2011921/150‑1 (MQ=60)
                                 CTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGA                                                                                                                    <  SRR19973061.1597731/150‑1 (MQ=60)
                                  TGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGAT                                                                                                                   >  SRR19973061.2244821/1‑150 (MQ=60)
                                  TGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAAAGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGAT                                                                                                                   <  SRR19973061.736176/150‑1 (MQ=60)
                                   GACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATG                                                                                                                  <  SRR19973061.2228259/150‑1 (MQ=60)
                                       GCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCC                                                                                                              <  SRR19973061.3356286/150‑1 (MQ=60)
                                        CATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCG                                                                                                             >  SRR19973061.1016386/1‑150 (MQ=60)
                                         ATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGT                                                                                                            >  SRR19973061.3588930/1‑150 (MQ=60)
                                           CGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAA                                                                                                          >  SRR19973061.1964454/1‑150 (MQ=60)
                                            GAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAAC                                                                                                         >  SRR19973061.2416297/1‑150 (MQ=60)
                                             AGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACT                                                                                                        <  SRR19973061.1468093/150‑1 (MQ=60)
                                                 CGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCAT                                                                                                    >  SRR19973061.2196608/1‑150 (MQ=60)
                                                    TGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGA                                                                                                 >  SRR19973061.1831195/1‑150 (MQ=60)
                                                     GTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGAC                                                                                                <  SRR19973061.2362699/150‑1 (MQ=60)
                                                     GTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGAC                                                                                                <  SRR19973061.2362810/150‑1 (MQ=60)
                                                     GTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGAC                                                                                                >  SRR19973061.1038771/1‑150 (MQ=60)
                                                            GCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCA                                                                                         <  SRR19973061.3443775/150‑1 (MQ=60)
                                                            GCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCA                                                                                         <  SRR19973061.3558955/150‑1 (MQ=60)
                                                             CGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCAT                                                                                        <  SRR19973061.3771303/150‑1 (MQ=60)
                                                             CGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCAT                                                                                        <  SRR19973061.1120052/150‑1 (MQ=60)
                                                             CGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCAT                                                                                        <  SRR19973061.1118661/150‑1 (MQ=60)
                                                              GAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATT                                                                                       >  SRR19973061.1762421/1‑150 (MQ=60)
                                                              GAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATT                                                                                       >  SRR19973061.2694542/1‑150 (MQ=60)
                                                               AGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGTCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTA                                                                                      >  SRR19973061.2798785/1‑150 (MQ=60)
                                                                 CTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAA                                                                                    >  SRR19973061.2144388/1‑150 (MQ=60)
                                                                 CTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAA                                                                                    >  SRR19973061.3740124/1‑150 (MQ=60)
                                                                     TCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAA                                                                                <  SRR19973061.1696622/150‑1 (MQ=60)
                                                                         CGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAAT                                                                            <  SRR19973061.1654427/150‑1 (MQ=60)
                                                                          GTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATC                                                                           <  SRR19973061.2194662/150‑1 (MQ=60)
                                                                            TGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAA                                                                         <  SRR19973061.2093427/150‑1 (MQ=60)
                                                                            TGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAA                                                                         >  SRR19973061.2395496/1‑150 (MQ=60)
                                                                              CGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATC                                                                       <  SRR19973061.996067/150‑1 (MQ=60)
                                                                              CGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATC                                                                       <  SRR19973061.3981/150‑1 (MQ=60)
                                                                              CGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATC                                                                       <  SRR19973061.4785/150‑1 (MQ=60)
                                                                              CGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATC                                                                       <  SRR19973061.4991/150‑1 (MQ=60)
                                                                                 AAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGG                                                                    <  SRR19973061.557535/150‑1 (MQ=60)
                                                                                 AAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGCTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACATCATTGACGCCATCATTAAAGAAAAAATCAATCCGG                                                                    >  SRR19973061.1605509/1‑150 (MQ=60)
                                                                                   AAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCT                                                                  <  SRR19973061.226743/150‑1 (MQ=60)
                                                                                   AAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCT                                                                  <  SRR19973061.2142832/150‑1 (MQ=60)
                                                                                    AAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTG                                                                 >  SRR19973061.134755/1‑150 (MQ=60)
                                                                                    AAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTG                                                                 <  SRR19973061.543830/150‑1 (MQ=60)
                                                                                         CGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCA                                                            >  SRR19973061.3122227/1‑150 (MQ=60)
                                                                                          GTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTAGCTCAGCGTTATGGCTCGTCTGTACTCCAGTACTTAATTTGAGACATGATGAACCGTAACTTCATTGACTCAATCATAAAAGAAAAAATCAATCCGTCTGGCGCAC                                                           >  SRR19973061.3728480/1‑150 (MQ=60)
                                                                                            ATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCG                                                         >  SRR19973061.3526840/1‑150 (MQ=60)
                                                                                            ATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCG                                                         >  SRR19973061.3526673/1‑150 (MQ=60)
                                                                                             TTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGA                                                        >  SRR19973061.2504333/1‑150 (MQ=60)
                                                                                                 CGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTA                                                    >  SRR19973061.3373743/1‑150 (MQ=60)
                                                                                                  GGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTAT                                                   <  SRR19973061.3461904/150‑1 (MQ=60)
                                                                                                  GGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTAT                                                   <  SRR19973061.1753936/150‑1 (MQ=60)
                                                                                                      TCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTC                                               <  SRR19973061.3185813/150‑1 (MQ=60)
                                                                                                      TCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTC                                               <  SRR19973061.3169280/150‑1 (MQ=60)
                                                                                                       CCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCC                                              <  SRR19973061.2337410/150‑1 (MQ=60)
                                                                                                       CCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCC                                              <  SRR19973061.2703008/150‑1 (MQ=60)
                                                                                                        CGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCG                                             >  SRR19973061.2772378/1‑150 (MQ=60)
                                                                                                          CAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGG                                           >  SRR19973061.2514854/1‑150 (MQ=60)
                                                                                                          CAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGG                                           >  SRR19973061.3282143/1‑150 (MQ=60)
                                                                                                          CAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGG                                           >  SRR19973061.2514443/1‑150 (MQ=60)
                                                                                                          CAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGG                                           >  SRR19973061.3557003/1‑150 (MQ=60)
                                                                                                           AAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGC                                          <  SRR19973061.1998680/150‑1 (MQ=60)
                                                                                                           AAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGC                                          >  SRR19973061.44367/1‑150 (MQ=60)
                                                                                                            AAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCG                                         >  SRR19973061.3775085/1‑150 (MQ=60)
                                                                                                              GGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAA                                       >  SRR19973061.489565/1‑150 (MQ=60)
                                                                                                                CAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATA                                     <  SRR19973061.2628379/150‑1 (MQ=60)
                                                                                                                CAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATA                                     <  SRR19973061.273424/150‑1 (MQ=60)
                                                                                                                   AGTGCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAA                                  >  SRR19973061.126914/1‑150 (MQ=60)
                                                                                                                      GCCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCT                               <  SRR19973061.2352681/150‑1 (MQ=60)
                                                                                                                       CCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTG                              <  SRR19973061.977604/150‑1 (MQ=60)
                                                                                                                       CCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTG                              <  SRR19973061.2858802/150‑1 (MQ=60)
                                                                                                                       CCAATGAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTG                              <  SRR19973061.2857467/150‑1 (MQ=60)
                                                                                                                            GAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGA                         <  SRR19973061.1882229/150‑1 (MQ=60)
                                                                                                                            GAATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGA                         >  SRR19973061.1761691/1‑150 (MQ=60)
                                                                                                                             AATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAA                        >  SRR19973061.1512069/1‑150 (MQ=60)
                                                                                                                              ATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAG                       >  SRR19973061.31937/1‑150 (MQ=60)
                                                                                                                              ATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAG                       >  SRR19973061.2449519/1‑150 (MQ=60)
                                                                                                                              ATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAG                       >  SRR19973061.1921346/1‑150 (MQ=60)
                                                                                                                              ATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAG                       >  SRR19973061.1432260/1‑150 (MQ=60)
                                                                                                                              ATATCGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAG                       >  SRR19973061.2449662/1‑150 (MQ=60)
                                                                                                                                  CGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTT                   <  SRR19973061.1032796/150‑1 (MQ=60)
                                                                                                                                  CGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTT                   <  SRR19973061.3246898/150‑1 (MQ=60)
                                                                                                                                  CGTTGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTT                   <  SRR19973061.3246720/150‑1 (MQ=60)
                                                                                                                                     TGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCAC                <  SRR19973061.929504/150‑1 (MQ=60)
                                                                                                                                     TGCTCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTAATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCAC                <  SRR19973061.1905416/150‑1 (MQ=60)
                                                                                                                                        TCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTA             <  SRR19973061.380527/150‑1 (MQ=60)
                                                                                                                                        TCAGCGTTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTA             <  SRR19973061.2292973/150‑1 (MQ=60)
                                                                                                                                              TTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTGT       >  SRR19973061.3699808/1‑150 (MQ=60)
                                                                                                                                              TTATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTGT       >  SRR19973061.1061846/1‑150 (MQ=60)
                                                                                                                                                ATGGCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTGTAG     >  SRR19973061.3123943/1‑150 (MQ=60)
                                                                                                                                                   GCTCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTGTAGAGT  <  SRR19973061.3195525/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
GCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTGTAGAGT  >  CP009273/450617‑450913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑