Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 659,689 G→T 85.3% R320S (CGC→AGC)  rlpA ← septal ring protein, suppressor of prc, minor lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273659,6890GT85.3% 396.1 / 77.7 184R320S (CGC→AGC) rlpAseptal ring protein, suppressor of prc, minor lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (13/14);  new base T (83/74);  total (96/88)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

Only 100 of 186 total aligned reads displayed.

GATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGC  >  CP009273/659543‑659833
                                                                                                                                                  |                                                                                                                                                
gATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCTAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCGa                                                                                                                                                 >  2:697894/1‑148 (MQ=255)
   gCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCg                                                                                                                                              <  1:1424883/148‑1 (MQ=255)
    ccTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCgg                                                                                                                                             <  2:3110953/148‑1 (MQ=255)
     cTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCggg                                                                                                                                            <  1:3060705/148‑1 (MQ=255)
       gCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGa                                                                                                                                          >  2:1761077/1‑148 (MQ=255)
         tttACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACg                                                                                                                                        <  2:2764630/148‑1 (MQ=255)
          ttACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGc                                                                                                                                       >  1:1110761/1‑148 (MQ=255)
           tACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGcc                                                                                                                                      >  2:1960456/1‑148 (MQ=255)
             cGCTACTGCGCGGTAGTAATAACTGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGa                                                                                                                                    <  1:2696717/148‑1 (MQ=255)
               cTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAAc                                                                                                                                  >  2:1549422/1‑148 (MQ=255)
                 aCTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACtt                                                                                                                                >  1:572132/1‑148 (MQ=255)
                 aCTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACtt                                                                                                                                <  2:722112/148‑1 (MQ=255)
                 aCTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCAGAACtt                                                                                                                                <  1:1174310/148‑1 (MQ=255)
                       cgGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTcc                                                                                                                          >  1:2582840/1‑148 (MQ=255)
                       cgGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTcc                                                                                                                          >  1:2583205/1‑148 (MQ=255)
                           agtaATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGt                                                                                                                      >  1:385833/1‑148 (MQ=255)
                           agtaATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGt                                                                                                                      >  1:2417984/1‑148 (MQ=255)
                           agtaATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGt                                                                                                                      >  1:385606/1‑148 (MQ=255)
                             taataaATGACTGTAAGTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTg                                                                                                                    <  2:3279162/148‑1 (MQ=255)
                               ataaATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCt                                                                                                                  >  2:1282477/1‑148 (MQ=255)
                                 aaaTGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGt                                                                                                                >  1:1028725/1‑148 (MQ=255)
                                   aTGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCTAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTg                                                                                                              >  2:2036337/1‑148 (MQ=255)
                                    tGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCTAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTgg                                                                                                             >  2:2310088/1‑148 (MQ=255)
                                     gACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGt                                                                                                            >  2:1313659/1‑148 (MQ=255)
                                       cTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTAc                                                                                                          >  2:3021057/1‑148 (MQ=255)
                                         gTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTg                                                                                                        <  2:1707083/148‑1 (MQ=255)
                                         gTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTg                                                                                                        <  1:2606126/148‑1 (MQ=255)
                                          tAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCTAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGt                                                                                                       >  1:3063083/1‑148 (MQ=255)
                                          tAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGt                                                                                                       <  2:2800151/148‑1 (MQ=255)
                                          tAATTGGGCTTCGGTGTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGt                                                                                                       <  2:2581813/148‑1 (MQ=255)
                                              tGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTgcg                                                                                                   >  1:203502/1‑148 (MQ=255)
                                              tGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTgcg                                                                                                   >  2:1200860/1‑148 (MQ=255)
                                                ggCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCa                                                                                                 <  2:2119719/148‑1 (MQ=255)
                                                   ttCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGa                                                                                              <  2:1795485/148‑1 (MQ=255)
                                                     cGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGc                                                                                            >  1:1834195/1‑148 (MQ=255)
                                                     cGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGc                                                                                            <  2:2440162/148‑1 (MQ=255)
                                                     cGGTTTGCAAACGTTGCTGCAAGCTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGc                                                                                            <  2:1516448/148‑1 (MQ=255)
                                                      ggTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGcc                                                                                           <  2:1331771/148‑1 (MQ=255)
                                                      ggTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGcc                                                                                           >  2:2906996/1‑148 (MQ=255)
                                                        tttGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTg                                                                                         >  1:116906/1‑148 (MQ=255)
                                                              aaCGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCt                                                                                   >  1:2259331/1‑148 (MQ=255)
                                                               aCGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCTAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCtt                                                                                  >  2:989099/1‑148 (MQ=255)
                                                               aCGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCTAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCtt                                                                                  >  2:1107358/1‑148 (MQ=255)
                                                               aCGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCtt                                                                                  <  1:878585/148‑1 (MQ=255)
                                                               aCGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCtt                                                                                  <  2:1432639/148‑1 (MQ=255)
                                                               aCGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCtt                                                                                  >  2:1155406/1‑148 (MQ=255)
                                                                cGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTa                                                                                 <  1:1988895/148‑1 (MQ=255)
                                                                     ctgcAAGGTACTGGCTTCGGGTTTGCTGGCTAATGGGCCTAGCTGGATCCGCCTGACCGCGCCATTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGTTCGCTTACGGcc                                                                            <  2:1570203/148‑1 (MQ=255)
                                                                      tgcAAGGTACTGGCTTCGGCTTTGCTGGCTAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGccc                                                                           <  2:80995/148‑1 (MQ=255)
                                                                       gcAAGGTACTGGCTTCGGCTTTGCTGGCTAATGGGCCAAGCTGGACCCGCCAGACCGCGCCATTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGcccc                                                                          >  2:2163883/1‑148 (MQ=255)
                                                                              aCTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGc                                                                   <  1:817098/148‑1 (MQ=255)
                                                                              aCTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGc                                                                   <  2:591593/148‑1 (MQ=255)
                                                                                tGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCAc                                                                 >  2:678621/1‑148 (MQ=255)
                                                                                    ttCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATa                                                             >  1:1517091/1‑148 (MQ=255)
                                                                                     tCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATaa                                                            >  1:1960181/1‑148 (MQ=255)
                                                                                     tCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATaa                                                            <  2:3091640/148‑1 (MQ=255)
                                                                                       ggCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAg                                                          <  2:421147/148‑1 (MQ=255)
                                                                                         cTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATCAAGtt                                                        >  1:1591431/1‑148 (MQ=255)
                                                                                         cTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGtt                                                        >  1:3230692/1‑148 (MQ=255)
                                                                                         cTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGtt                                                        >  1:3231020/1‑148 (MQ=255)
                                                                                         cTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGtt                                                        >  2:164583/1‑148 (MQ=255)
                                                                                         cTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGtt                                                        >  1:2729464/1‑148 (MQ=255)
                                                                                           ttGCTGGCTAATGGGCCAAGCTGGATCCGCCAGACCGCGCCTTTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGc                                                      <  1:943482/148‑1 (MQ=255)
                                                                                           ttGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTAAGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTAGCACCATAAAGATGc                                                      >  2:1418970/1‑148 (MQ=255)
                                                                                                ggCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTg                                                 >  1:2847127/1‑148 (MQ=255)
                                                                                                ggCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTg                                                 >  2:907676/1‑148 (MQ=255)
                                                                                                  ctaaTGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCAGCAAGTTGCCGCTGgc                                               >  2:1747456/3‑148 (MQ=255)
                                                                                                   gAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGgcg                                              <  1:1667500/148‑1 (MQ=255)
                                                                                                    aaTGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGACCTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGgcgc                                             >  2:1689588/1‑148 (MQ=255)
                                                                                                    aaTGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGACCTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGgcgc                                             >  2:13846/1‑148 (MQ=255)
                                                                                                    aaTGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGgcgc                                             >  1:1739/1‑148 (MQ=255)
                                                                                                     aTGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCt                                            <  1:1021026/148‑1 (MQ=255)
                                                                                                     aTGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCt                                            >  1:2137203/1‑148 (MQ=255)
                                                                                                     aTGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCt                                            <  2:1517091/148‑1 (MQ=255)
                                                                                                        ggCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTg                                         <  2:1228304/148‑1 (MQ=255)
                                                                                                        ggCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTg                                         <  2:1228346/148‑1 (MQ=255)
                                                                                                          ccAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCg                                       >  1:3116648/1‑148 (MQ=255)
                                                                                                             aGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCgaga                                    >  1:829879/1‑148 (MQ=255)
                                                                                                             aGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCgaga                                    >  1:829829/1‑148 (MQ=255)
                                                                                                                tGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACgg                                 <  1:1761089/148‑1 (MQ=255)
                                                                                                                 ggATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGc                                <  2:2294614/148‑1 (MQ=255)
                                                                                                                 ggATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGc                                <  1:494604/148‑1 (MQ=255)
                                                                                                                 ggATCCGCCAGACCGCGCCATTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGc                                <  1:697894/148‑1 (MQ=255)
                                                                                                                  gATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCt                                            <  1:139692/135‑1 (MQ=255)
                                                                                                                  gATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCt                               <  1:3281224/148‑1 (MQ=255)
                                                                                                                                cgcCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCAt                 <  2:572132/148‑1 (MQ=255)
                                                                                                                                  ccATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTg               >  2:1318411/1‑148 (MQ=255)
                                                                                                                                   cATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGc              >  2:39494/1‑148 (MQ=255)
                                                                                                                                    aTTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCa             <  2:1176583/148‑1 (MQ=255)
                                                                                                                                    aTTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCa             <  2:844862/148‑1 (MQ=255)
                                                                                                                                      tttGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAgg           >  2:780819/1‑148 (MQ=255)
                                                                                                                                      tttGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAgg           >  2:2109084/1‑148 (MQ=255)
                                                                                                                                      tttGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAgg           >  1:790256/1‑148 (MQ=255)
                                                                                                                                          aGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAg       <  2:3188216/148‑1 (MQ=255)
                                                                                                                                          aGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAg       >  2:2058579/1‑148 (MQ=255)
                                                                                                                                           gTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAgg      <  1:2036416/148‑1 (MQ=255)
                                                                                                                                            ttACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGt     <  2:3030639/148‑1 (MQ=255)
                                                                                                                                            ttACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGt     >  1:3265794/1‑148 (MQ=255)
                                                                                                                                             tACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGtt    >  1:2749132/1‑148 (MQ=255)
                                                                                                                                               cgctACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGc  <  1:1200860/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGC  >  CP009273/659543‑659833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGC  >  CP009273/659543‑659837
                                                                                                                                                  |                                                                                                                                                    
GATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACC                                                                                                                                                   <  SRR19973070.3169013/150‑1 (MQ=60)
   GCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGG                                                                                                                                                <  SRR19973070.1434691/150‑1 (MQ=60)
    CCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGG                                                                                                                                               >  SRR19973070.1548490/1‑150 (MQ=60)
     CTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGA                                                                                                                                              <  SRR19973070.3080292/150‑1 (MQ=60)
        CTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGC                                                                                                                                           >  SRR19973070.1118360/1‑150 (MQ=60)
             CGCTACTGCGCGGTAGTAATAACTGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAAC                                                                                                                                      <  SRR19973070.2714176/150‑1 (MQ=60)
              GCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACT                                                                                                                                     <  SRR19973070.3079504/150‑1 (MQ=60)
               CTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTT                                                                                                                                    >  SRR19973070.576029/1‑150 (MQ=60)
               CTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTT                                                                                                                                    <  SRR19973070.113733/150‑1 (MQ=60)
                 ACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCAGAACTTCT                                                                                                                                  <  SRR19973070.1178678/150‑1 (MQ=60)
                 ACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCAGAACTTCT                                                                                                                                  <  SRR19973070.1182540/150‑1 (MQ=60)
                     CGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCC                                                                                                                              >  SRR19973070.2600089/1‑150 (MQ=60)
                     CGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCC                                                                                                                              >  SRR19973070.2599722/1‑150 (MQ=60)
                         GTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGT                                                                                                                          >  SRR19973070.388433/1‑150 (MQ=60)
                         GTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGT                                                                                                                          >  SRR19973070.818083/1‑150 (MQ=60)
                         GTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGT                                                                                                                          >  SRR19973070.2433897/1‑150 (MQ=60)
                         GTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGT                                                                                                                          >  SRR19973070.388663/1‑150 (MQ=60)
                               ATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGT                                                                                                                    >  SRR19973070.1036011/1‑150 (MQ=60)
                                  AATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGG                                                                                                                 >  SRR19973070.1066526/1‑150 (MQ=60)
                                     GACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTAC                                                                                                              >  SRR19973070.2702168/1‑150 (MQ=60)
                                      ACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACT                                                                                                             <  SRR19973070.3125230/150‑1 (MQ=60)
                                        TGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCTAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGT                                                                                                           >  SRR19973070.3082700/1‑150 (MQ=60)
                                         GTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTT                                                                                                          <  SRR19973070.2623138/150‑1 (MQ=60)
                                            ATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCG                                                                                                       >  SRR19973070.205033/1‑150 (MQ=60)
                                            ATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCG                                                                                                       >  SRR19973070.509209/1‑150 (MQ=60)
                                            ATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCG                                                                                                       >  SRR19973070.1885511/1‑150 (MQ=60)
                                                GGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACG                                                                                                   <  SRR19973070.396277/150‑1 (MQ=60)
                                                   TTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGC                                                                                                >  SRR19973070.1846804/1‑150 (MQ=60)
                                                   TTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGC                                                                                                <  SRR19973070.2025712/150‑1 (MQ=60)
                                                      GGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTG                                                                                             >  SRR19973070.792495/1‑150 (MQ=60)
                                                      GGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTG                                                                                             >  SRR19973070.117965/1‑150 (MQ=60)
                                                            CAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCT                                                                                       >  SRR19973070.2274318/1‑150 (MQ=60)
                                                               ACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTAC                                                                                    <  SRR19973070.884772/150‑1 (MQ=60)
                                                                CGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACG                                                                                   <  SRR19973070.2002365/150‑1 (MQ=60)
                                                                      TGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCG                                                                             <  SRR19973070.317459/150‑1 (MQ=60)
                                                                              ACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCAC                                                                     <  SRR19973070.822680/150‑1 (MQ=60)
                                                                                  GCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATA                                                                 >  SRR19973070.1527727/1‑150 (MQ=60)
                                                                                   CTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAA                                                                >  SRR19973070.1973388/1‑150 (MQ=60)
                                                                                   CTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAA                                                                >  SRR19973070.2063547/1‑150 (MQ=60)
                                                                                       GGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATCAAGTT                                                            >  SRR19973070.1602558/1‑150 (MQ=60)
                                                                                       GGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTT                                                            >  SRR19973070.2748076/1‑150 (MQ=60)
                                                                                       GGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTT                                                            >  SRR19973070.2747036/1‑150 (MQ=60)
                                                                                       GGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTT                                                            <  SRR19973070.1394083/150‑1 (MQ=60)
                                                                                       GGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTT                                                            >  SRR19973070.2747060/1‑150 (MQ=60)
                                                                                       GGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTT                                                            >  SRR19973070.3251885/1‑150 (MQ=60)
                                                                                       GGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTT                                                            >  SRR19973070.3251822/1‑150 (MQ=60)
                                                                                       GGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTT                                                            >  SRR19973070.3251556/1‑150 (MQ=60)
                                                                                           TTGCTGGCTAATGGGCCAAGCTGGATCCGCCAGACCGCGCCTTTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCG                                                        <  SRR19973070.950202/150‑1 (MQ=60)
                                                                                           TTGCTGGCTAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCG                                                        <  SRR19973070.949455/150‑1 (MQ=60)
                                                                                             GCTGGCTAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCT                                                      <  SRR19973070.3253434/150‑1 (MQ=60)
                                                                                              CTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTG                                                     >  SRR19973070.1204920/1‑150 (MQ=60)
                                                                                              CTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTG                                                     >  SRR19973070.2865562/1‑150 (MQ=60)
                                                                                                  CGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGC                                                 >  SRR19973070.1769/1‑150 (MQ=60)
                                                                                                   GAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCT                                                <  SRR19973070.2298945/150‑1 (MQ=60)
                                                                                                   GAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCT                                                >  SRR19973070.2151467/1‑150 (MQ=60)
                                                                                                   GAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCT                                                <  SRR19973070.3324679/150‑1 (MQ=60)
                                                                                                   GAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCT                                                <  SRR19973070.1678952/150‑1 (MQ=60)
                                                                                                    AATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTT                                               <  SRR19973070.2270934/150‑1 (MQ=60)
                                                                                                    AATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTT                                               >  SRR19973070.1773316/1‑150 (MQ=60)
                                                                                                     tcttccgatctTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTT                                              <  SRR19973070.140906/139‑1 (MQ=60)
                                                                                                     ATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTT                                              <  SRR19973070.2522094/150‑1 (MQ=60)
                                                                                                     ATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTT                                              <  SRR19973070.1028274/150‑1 (MQ=60)
                                                                                                     ATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTT                                              <  SRR19973070.855810/150‑1 (MQ=60)
                                                                                                        GGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCG                                           >  SRR19973070.3136758/1‑150 (MQ=60)
                                                                                                           CAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGA                                        >  SRR19973070.835512/1‑150 (MQ=60)
                                                                                                           CAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGA                                        >  SRR19973070.835462/1‑150 (MQ=60)
                                                                                                             AGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACG                                      <  SRR19973070.1425187/150‑1 (MQ=60)
                                                                                                                TGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCT                                   <  SRR19973070.1773267/150‑1 (MQ=60)
                                                                                                                TGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCT                                   <  SRR19973070.1773279/150‑1 (MQ=60)
                                                                                                                 GGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTT                                  <  SRR19973070.1659435/150‑1 (MQ=60)
                                                                                                                 GGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTT                                  <  SRR19973070.498059/150‑1 (MQ=60)
                                                                                                                 GGATCCGCCAGACCGCGCCATTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTT                                  <  SRR19973070.702869/150‑1 (MQ=60)
                                                                                                                  GATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTG                                 <  SRR19973070.3302298/150‑1 (MQ=60)
                                                                                                                    TCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCG                               <  SRR19973070.1738080/150‑1 (MQ=60)
                                                                                                                         CAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTC                          >  SRR19973070.822692/1‑150 (MQ=60)
                                                                                                                                    ATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGG               >  SRR19973070.795735/1‑150 (MQ=60)
                                                                                                                                          AGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGT         >  SRR19973070.2603770/1‑150 (MQ=60)
                                                                                                                                          AGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGT         >  SRR19973070.3286821/1‑150 (MQ=60)
                                                                                                                                          AGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGT         >  SRR19973070.3286809/1‑150 (MQ=60)
                                                                                                                                          AGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGT         <  SRR19973070.2862598/150‑1 (MQ=60)
                                                                                                                                           GTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTT        >  SRR19973070.2766795/1‑150 (MQ=60)
                                                                                                                                           GTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTT        <  SRR19973070.2050192/150‑1 (MQ=60)
                                                                                                                                           GTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTT        <  SRR19973070.2050111/150‑1 (MQ=60)
                                                                                                                                               CGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAG    <  SRR19973070.602411/150‑1 (MQ=60)
                                                                                                                                               CGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAG    <  SRR19973070.1209293/150‑1 (MQ=60)
                                                                                                                                                 CTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGC  >  SRR19973070.1000058/1‑150 (MQ=60)
                                                                                                                                                 CTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGC  >  SRR19973070.1000064/1‑150 (MQ=60)
                                                                                                                                                 CTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGC  >  SRR19973070.1000061/1‑150 (MQ=60)
                                                                                                                                                  |                                                                                                                                                    
GATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGGC  >  CP009273/659543‑659837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑