Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 659,689 G→T 100% R320S (CGC→AGC)  rlpA ← septal ring protein, suppressor of prc, minor lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273659,6890GT99.5% 634.3 / ‑4.3 198R320S (CGC→AGC) rlpAseptal ring protein, suppressor of prc, minor lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (89/108);  total (89/109)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 199 total aligned reads displayed.

ATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGC  >  CP009273/659544‑659833
                                                                                                                                                 |                                                                                                                                                
aTGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTAc                                                                                                                                                >  1:1630553/1‑148 (MQ=255)
    cTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCggg                                                                                                                                            <  1:3256714/148‑1 (MQ=255)
    cTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCggg                                                                                                                                            >  2:1927541/1‑148 (MQ=255)
     tGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCgggg                                                                                                                                           <  2:2584040/148‑1 (MQ=255)
      gCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGa                                                                                                                                          >  1:2876603/1‑148 (MQ=255)
         ttACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGc                                                                                                                                       <  1:2124956/148‑1 (MQ=255)
         ttACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGc                                                                                                                                       <  2:345838/148‑1 (MQ=255)
            cGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGa                                                                                                                                    >  2:1652920/1‑148 (MQ=255)
              cTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAAc                                                                                                                                  >  1:1132163/1‑148 (MQ=255)
                 cTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTc                                                                                                                               <  1:65116/148‑1 (MQ=255)
                     gcgGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTc                                                                                                                           <  2:3401981/148‑1 (MQ=255)
                         tagtaATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAg                                                                                                                       <  2:2841469/148‑1 (MQ=255)
                         tagtaATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAg                                                                                                                       >  1:1246062/1‑148 (MQ=255)
                          agtaATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGt                                                                                                                      <  2:492817/148‑1 (MQ=255)
                            taataaATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTg                                                                                                                    >  2:2175162/1‑148 (MQ=255)
                               taaATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTg                                                                                                                 <  1:1885605/148‑1 (MQ=255)
                               taaATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTg                                                                                                                 <  2:645430/148‑1 (MQ=255)
                                aaaTGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGt                                                                                                                >  2:3057613/1‑148 (MQ=255)
                                aaaTGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGt                                                                                                                <  1:2242005/148‑1 (MQ=255)
                                  aTGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTg                                                                                                              >  2:191827/1‑148 (MQ=255)
                                   tGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTgg                                                                                                             >  2:419833/1‑148 (MQ=255)
                                     aCTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTa                                                                                                           >  2:1477723/1‑148 (MQ=255)
                                        gTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTg                                                                                                        >  2:1821290/1‑148 (MQ=255)
                                        gTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTg                                                                                                        >  1:3213173/1‑148 (MQ=255)
                                         tAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGt                                                                                                       >  2:1153418/1‑148 (MQ=255)
                                           aTTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTg                                                                                                     >  2:3293617/1‑148 (MQ=255)
                                            ttGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTgc                                                                                                    >  2:2748405/1‑148 (MQ=255)
                                            ttGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTgc                                                                                                    >  2:995298/1‑148 (MQ=255)
                                             tGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTgcg                                                                                                   >  1:182401/1‑148 (MQ=255)
                                                    cGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGc                                                                                            >  1:2566870/1‑148 (MQ=255)
                                                    cGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGc                                                                                            <  1:666474/148‑1 (MQ=255)
                                                     ggTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGcc                                                                                           <  1:1975996/148‑1 (MQ=255)
                                                        ttGCAAACGTTGCTTCAAGGTACTGGCTTCGGCTTTGCTGGAGAATGGGACAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCtta                                                                                        <  1:1058317/148‑3 (MQ=255)
                                                        ttGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGa                                                                                        <  2:2489507/148‑1 (MQ=255)
                                                            aaaCGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGc                                                                                    <  2:2051769/148‑1 (MQ=255)
                                                              aCGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCg                                                                                     <  1:750624/145‑1 (MQ=255)
                                                              aCGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCg                                                                                     >  2:750594/1‑145 (MQ=255)
                                                              aCGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCtt                                                                                  <  1:3196207/148‑1 (MQ=255)
                                                              aCGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCtt                                                                                  >  2:1537783/1‑148 (MQ=255)
                                                              aCGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCtt                                                                                  <  1:2319528/148‑1 (MQ=255)
                                                               cGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGACCTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTa                                                                                 >  1:305190/1‑148 (MQ=255)
                                                               cGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTa                                                                                 >  1:1219553/1‑148 (MQ=255)
                                                                    ctgcAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGcc                                                                            <  1:981514/148‑1 (MQ=255)
                                                                     tgcAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGccc                                                                           <  1:3428659/148‑1 (MQ=255)
                                                                     tgcAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGccc                                                                           <  1:2835975/148‑1 (MQ=255)
                                                                      gcAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGcccc                                                                          >  1:2048/1‑148 (MQ=255)
                                                                        aaGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGa                                                                        <  1:206648/148‑1 (MQ=255)
                                                                            tACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTg                                                                    >  2:668793/1‑148 (MQ=255)
                                                                            tACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTg                                                                    >  2:848258/1‑148 (MQ=255)
                                                                            tACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTg                                                                    <  1:1768259/148‑1 (MQ=255)
                                                                             aCTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGc                                                                   <  2:1240083/148‑1 (MQ=255)
                                                                               tGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGACCTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCAc                                                                 >  2:1640565/1‑148 (MQ=255)
                                                                               tGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCAc                                                                 >  2:3389877/1‑148 (MQ=255)
                                                                               tGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGACAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCAc                                                                 <  1:3271936/148‑1 (MQ=255)
                                                                                 gCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCa                                                               <  2:2289352/148‑1 (MQ=255)
                                                                                  cTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCAt                                                              >  2:2122559/1‑148 (MQ=255)
                                                                                  cTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCAt                                                              <  1:1927170/148‑1 (MQ=255)
                                                                                   ttCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATa                                                             >  1:626419/1‑148 (MQ=255)
                                                                                   ttCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATa                                                             >  1:626309/1‑148 (MQ=255)
                                                                                    tCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATaa                                                            <  1:2594623/148‑1 (MQ=255)
                                                                                    tCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATaa                                                            <  1:1978603/148‑1 (MQ=255)
                                                                                    tCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATaa                                                            >  1:849528/1‑148 (MQ=255)
                                                                                    tCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATaa                                                            <  1:823422/148‑1 (MQ=255)
                                                                                    tCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATaa                                                            <  1:2445221/148‑1 (MQ=255)
                                                                                      ggCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGCACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAg                                                          <  2:3170506/148‑1 (MQ=255)
                                                                                       gCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGt                                                         <  2:1832538/148‑1 (MQ=255)
                                                                                       gCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGt                                                         <  1:20061/148‑1 (MQ=255)
                                                                                       gCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGt                                                         <  1:882057/148‑1 (MQ=255)
                                                                                         tttGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTg                                                       <  2:3312755/148‑1 (MQ=255)
                                                                                         tttGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTg                                                       <  1:1819847/148‑1 (MQ=255)
                                                                                            gCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCg                                                    <  2:2417624/148‑1 (MQ=255)
                                                                                            gCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGAGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCg                                                    <  1:1293549/148‑1 (MQ=255)
                                                                                             cTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGc                                                   <  2:2621576/148‑1 (MQ=255)
                                                                                                  gAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGgcg                                              <  2:51978/148‑1 (MQ=255)
                                                                                                   aaTGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGgcgc                                             <  1:1450286/148‑1 (MQ=255)
                                                                                                   aaTGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGgcgc                                             <  2:356166/148‑1 (MQ=255)
                                                                                                     tGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCtt                                           <  1:2672573/148‑1 (MQ=255)
                                                                                                     tGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCtt                                           >  2:215447/1‑148 (MQ=255)
                                                                                                            aGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCgaga                                    >  2:862226/1‑148 (MQ=255)
                                                                                                            aGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCgaga                                    <  1:1337386/148‑1 (MQ=255)
                                                                                                              cTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACg                                  >  1:2042926/1‑148 (MQ=255)
                                                                                                                ggATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGc                                <  2:118398/148‑1 (MQ=255)
                                                                                                                 gATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCt                               <  1:2766774/148‑1 (MQ=255)
                                                                                                                  aTCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCtt                              <  2:1719849/148‑1 (MQ=255)
                                                                                                                       ccAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGgt                         >  1:239362/1‑148 (MQ=255)
                                                                                                                       ccAGACCGCGCCAGTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGgt                         >  1:1233869/1‑148 (MQ=255)
                                                                                                                         aGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGgtgt                       <  2:1416105/148‑1 (MQ=255)
                                                                                                                          gACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTc                      <  1:3189442/148‑1 (MQ=255)
                                                                                                                           aCCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCa                     >  2:1257193/1‑148 (MQ=255)
                                                                                                                            ccGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCAc                    <  1:2625400/148‑1 (MQ=255)
                                                                                                                                 ccATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTg               <  1:1652920/148‑1 (MQ=255)
                                                                                                                                 ccATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTg               >  2:2447202/1‑148 (MQ=255)
                                                                                                                                     tttGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAgg           >  2:3332488/1‑148 (MQ=255)
                                                                                                                                        gAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGa        >  1:734443/1‑148 (MQ=255)
                                                                                                                                          gTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAgg      <  1:528946/148‑1 (MQ=255)
                                                                                                                                          gTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAgg      <  1:1424261/148‑1 (MQ=255)
                                                                                                                                          gTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAgg      <  2:701564/148‑1 (MQ=255)
                                                                                                                                           ttACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGt     <  2:3254112/148‑1 (MQ=255)
                                                                                                                                             aCGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTg   <  2:2876603/148‑1 (MQ=255)
                                                                                                                                              cgctACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGc  <  2:132889/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGC  >  CP009273/659544‑659833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCA  >  CP009273/659540‑659834
                                                                                                                                                     |                                                                                                                                                 
TCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGACCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCT                                                                                                                                                   >  SRR19973065.3459199/1‑150 (MQ=60)
 CAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTA                                                                                                                                                  <  SRR19973065.2414159/150‑1 (MQ=60)
 CAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTA                                                                                                                                                  <  SRR19973065.2296785/150‑1 (MQ=60)
  AGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTAC                                                                                                                                                 >  SRR19973065.2303772/1‑150 (MQ=60)
  AGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTAC                                                                                                                                                 >  SRR19973065.1641487/1‑150 (MQ=60)
   GATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACC                                                                                                                                                <  SRR19973065.919745/150‑1 (MQ=60)
   GATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACC                                                                                                                                                <  SRR19973065.2686599/150‑1 (MQ=60)
   GATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACC                                                                                                                                                <  SRR19973065.1628084/150‑1 (MQ=60)
    ATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCG                                                                                                                                               <  SRR19973065.3148879/150‑1 (MQ=60)
        CTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGA                                                                                                                                           >  SRR19973065.2893580/1‑150 (MQ=60)
        CTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGA                                                                                                                                           >  SRR19973065.2894222/1‑150 (MQ=60)
        CTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGA                                                                                                                                           <  SRR19973065.3276415/150‑1 (MQ=60)
        CTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGA                                                                                                                                           <  SRR19973065.3276861/150‑1 (MQ=60)
           CTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGC                                                                                                                                        >  SRR19973065.3273786/1‑150 (MQ=60)
            TTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCC                                                                                                                                       <  SRR19973065.204727/150‑1 (MQ=60)
             TTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCG                                                                                                                                      <  SRR19973065.2138634/150‑1 (MQ=60)
                CGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAAC                                                                                                                                   >  SRR19973065.1139511/1‑150 (MQ=60)
                CGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAAC                                                                                                                                   >  SRR19973065.1436885/1‑150 (MQ=60)
                 GCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACT                                                                                                                                  <  SRR19973065.1927983/150‑1 (MQ=60)
                     CTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTG                                                                                                                              <  SRR19973065.65543/150‑1 (MQ=60)
                     CTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTG                                                                                                                              <  SRR19973065.65811/150‑1 (MQ=60)
                           GGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAG                                                                                                                        >  SRR19973065.1254269/1‑150 (MQ=60)
                           GGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAG                                                                                                                        >  SRR19973065.573427/1‑150 (MQ=60)
                           GGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAG                                                                                                                        >  SRR19973065.67402/1‑150 (MQ=60)
                           GGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAG                                                                                                                        >  SRR19973065.1858285/1‑150 (MQ=60)
                            GTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGT                                                                                                                       <  SRR19973065.2929766/150‑1 (MQ=60)
                                   TAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTT                                                                                                                <  SRR19973065.1898015/150‑1 (MQ=60)
                                   TAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTT                                                                                                                <  SRR19973065.2834216/150‑1 (MQ=60)
                                    AAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTG                                                                                                               <  SRR19973065.2256249/150‑1 (MQ=60)
                                    AAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTG                                                                                                               <  SRR19973065.2425026/150‑1 (MQ=60)
                                     AATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGG                                                                                                              >  SRR19973065.2637803/1‑150 (MQ=60)
                                          CTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTG                                                                                                         >  SRR19973065.3232501/1‑150 (MQ=60)
                                            GTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTT                                                                                                       <  SRR19973065.1697399/150‑1 (MQ=60)
                                               ATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCG                                                                                                    >  SRR19973065.183842/1‑150 (MQ=60)
                                               ATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCG                                                                                                    >  SRR19973065.1970153/1‑150 (MQ=60)
                                                  GGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCAC                                                                                                 >  SRR19973065.2676849/1‑150 (MQ=60)
                                                      TTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGC                                                                                             >  SRR19973065.2583012/1‑150 (MQ=60)
                                                      TTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGC                                                                                             >  SRR19973065.2582900/1‑150 (MQ=60)
                                                        CGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCT                                                                                           <  SRR19973065.670910/150‑1 (MQ=60)
                                                         GGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTG                                                                                          <  SRR19973065.1988856/150‑1 (MQ=60)
                                                          GTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGA                                                                                         >  SRR19973065.2135588/1‑150 (MQ=60)
                                                            TTGCAAACGTTGCTTCAAGGTACTGGCTTCGGCTTTGCTGGAGAATGGGACAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTTATC                                                                                       <  SRR19973065.1065334/150‑1 (MQ=60)
                                                               TAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCT                                                                                    <  SRR19973065.755603/150‑1 (MQ=60)
                                                               TAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCT                                                                                    <  SRR19973065.755633/150‑1 (MQ=60)
                                                                 AACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGACCTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTA                                                                                  >  SRR19973065.307211/1‑150 (MQ=60)
                                                                 AACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTA                                                                                  >  SRR19973065.827355/1‑150 (MQ=60)
                                                                 AACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTA                                                                                  >  SRR19973065.1227480/1‑150 (MQ=60)
                                                                 AACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTA                                                                                  >  SRR19973065.1227478/1‑150 (MQ=60)
                                                                  ACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTAC                                                                                 <  SRR19973065.3215467/150‑1 (MQ=60)
                                                                  ACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTAC                                                                                 <  SRR19973065.2334064/150‑1 (MQ=60)
                                                                  ACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTAC                                                                                 <  SRR19973065.3215989/150‑1 (MQ=60)
                                                                        CTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCC                                                                           <  SRR19973065.988030/150‑1 (MQ=60)
                                                                        CTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCC                                                                           >  SRR19973065.2070/1‑150 (MQ=60)
                                                                         TGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCG                                                                          <  SRR19973065.3449579/150‑1 (MQ=60)
                                                                         TGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCG                                                                          <  SRR19973065.2853438/150‑1 (MQ=60)
                                                                            AAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACT                                                                       <  SRR19973065.208217/150‑1 (MQ=60)
                                                                              GGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTG                                                                     <  SRR19973065.1656245/150‑1 (MQ=60)
                                                                               GTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGC                                                                    <  SRR19973065.2058733/150‑1 (MQ=60)
                                                                                TACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCA                                                                   <  SRR19973065.1779848/150‑1 (MQ=60)
                                                                                TACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCA                                                                   >  SRR19973065.1609706/1‑150 (MQ=60)
                                                                                   TGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCA                                                                >  SRR19973065.1306383/1‑150 (MQ=60)
                                                                                   TGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGACAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCA                                                                <  SRR19973065.3291791/150‑1 (MQ=60)
                                                                                     GCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATA                                                              >  SRR19973065.630375/1‑150 (MQ=60)
                                                                                     GCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATA                                                              >  SRR19973065.630378/1‑150 (MQ=60)
                                                                                     GCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATA                                                              >  SRR19973065.630487/1‑150 (MQ=60)
                                                                                      CTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAA                                                             >  SRR19973065.854806/1‑150 (MQ=60)
                                                                                      CTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAA                                                             <  SRR19973065.1939797/150‑1 (MQ=60)
                                                                                      CTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAA                                                             >  SRR19973065.855034/1‑150 (MQ=60)
                                                                                        TCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGTCGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAG                                                           <  SRR19973065.1992576/150‑1 (MQ=60)
                                                                                        TCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAG                                                           <  SRR19973065.2460630/150‑1 (MQ=60)
                                                                                        TCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAG                                                           <  SRR19973065.1991473/150‑1 (MQ=60)
                                                                                        TCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAG                                                           <  SRR19973065.1991361/150‑1 (MQ=60)
                                                                                        TCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAG                                                           <  SRR19973065.2460632/150‑1 (MQ=60)
                                                                                        TCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAG                                                           <  SRR19973065.828824/150‑1 (MQ=60)
                                                                                        TCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAG                                                           <  SRR19973065.2610754/150‑1 (MQ=60)
                                                                                           GCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTG                                                        <  SRR19973065.20342/150‑1 (MQ=60)
                                                                                           GCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTG                                                        <  SRR19973065.397977/150‑1 (MQ=60)
                                                                                           GCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTG                                                        <  SRR19973065.887635/150‑1 (MQ=60)
                                                                                           GCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTG                                                        <  SRR19973065.887675/150‑1 (MQ=60)
                                                                                             TTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCC                                                      <  SRR19973065.1831651/150‑1 (MQ=60)
                                                                                             TTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCC                                                      <  SRR19973065.1831830/150‑1 (MQ=60)
                                                                                                GCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGAGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCT                                                   <  SRR19973065.1302073/150‑1 (MQ=60)
                                                                                                GCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGAGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCT                                                   <  SRR19973065.1302071/150‑1 (MQ=60)
                                                                                                     CGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGC                                              >  SRR19973065.2339166/1‑150 (MQ=60)
                                                                                                     CGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGC                                              >  SRR19973065.3209742/1‑150 (MQ=60)
                                                                                                       AATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTT                                            <  SRR19973065.1459647/150‑1 (MQ=60)
                                                                                                         TGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTG                                          <  SRR19973065.2689135/150‑1 (MQ=60)
                                                                                                                AGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACG                                   <  SRR19973065.2072876/150‑1 (MQ=60)
                                                                                                                AGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACG                                   <  SRR19973065.1346287/150‑1 (MQ=60)
                                                                                                                AGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACG                                   <  SRR19973065.3443170/150‑1 (MQ=60)
                                                                                                                AGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACG                                   <  SRR19973065.1346244/150‑1 (MQ=60)
                                                                                                                AGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACG                                   >  SRR19973065.2056001/1‑150 (MQ=60)
                                                                                                                     GATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTG                              <  SRR19973065.2783824/150‑1 (MQ=60)
                                                                                                                     GATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTG                              <  SRR19973065.2783972/150‑1 (MQ=60)
                                                                                                                         CGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGT                          >  SRR19973065.241077/1‑150 (MQ=60)
                                                                                                                         CGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGT                          >  SRR19973065.1241974/1‑150 (MQ=60)
                                                                                                                         CGCCAGACCGCGCCAGTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGT                          >  SRR19973065.1241950/1‑150 (MQ=60)
                                                                                                                          GCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTG                         <  SRR19973065.1833283/150‑1 (MQ=60)
                                                                                                                              GACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCAC                     <  SRR19973065.3208684/150‑1 (MQ=60)
                                                                                                                                CCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCA                   <  SRR19973065.2641642/150‑1 (MQ=60)
                                                                                                                                     CCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCA              >  SRR19973065.658691/1‑150 (MQ=60)
                                                                                                                                     CCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCA              <  SRR19973065.1664005/150‑1 (MQ=60)
                                                                                                                                     CCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCA              <  SRR19973065.2180813/150‑1 (MQ=60)
                                                                                                                                      CATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAG             >  SRR19973065.958880/1‑150 (MQ=60)
                                                                                                                                          TTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGA         >  SRR19973065.739356/1‑150 (MQ=60)
                                                                                                                                              GTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTT     <  SRR19973065.1433544/150‑1 (MQ=60)
                                                                                                                                              GTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTT     <  SRR19973065.532412/150‑1 (MQ=60)
                                                                                                                                                 ACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAACCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCA  <  SRR19973065.1222950/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                 
TCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCA  >  CP009273/659540‑659834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑