Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 659,689 G→T 100% R320S (CGC→AGC)  rlpA ← septal ring protein, suppressor of prc, minor lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273659,6890GT95.0% 518.9 / 14.3 181R320S (CGC→AGC) rlpAseptal ring protein, suppressor of prc, minor lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (6/4);  new base T (68/103);  total (74/107)
Fisher's exact test for biased strand distribution p-value = 3.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 182 total aligned reads displayed.

ATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGC  >  CP009273/659544‑659833
                                                                                                                                                 |                                                                                                                                                
aTGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTAc                                                                                                                                                <  1:2273168/148‑1 (MQ=255)
aTGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTAc                                                                                                                                                >  2:1161721/1‑148 (MQ=255)
aTGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTAc                                                                                                                                                >  1:3132710/1‑148 (MQ=255)
aTGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTAc                                                                                                                                                <  1:479927/148‑1 (MQ=255)
 tGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTAcc                                                                                                                                               >  1:2723750/1‑148 (MQ=255)
    cTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCggg                                                                                                                                            >  1:2400428/1‑148 (MQ=255)
      gCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGa                                                                                                                                          <  1:1384109/148‑1 (MQ=255)
      gCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGa                                                                                                                                          >  2:2393932/1‑148 (MQ=255)
         ttACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGc                                                                                                                                       >  1:392123/1‑148 (MQ=255)
            cGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGa                                                                                                                                    <  2:1608374/148‑1 (MQ=255)
             gCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGaa                                                                                                                                   <  2:1759505/148‑1 (MQ=255)
              cTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAAc                                                                                                                                  <  1:3059710/148‑1 (MQ=255)
               tACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACt                                                                                                                                 >  2:585743/1‑148 (MQ=255)
                aCTGCGCGGTAGTAATAAATGACTGAAAACCCCCCGCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACtt                                                                                                                                <  2:3395746/148‑1 (MQ=255)
                 cTGCGCGGTAGTAATAAAGGACTGTAATTGGGCTTCGGTGTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTc                                                                                                                               <  2:2140394/148‑1 (MQ=255)
                   gcgcGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTg                                                                                                                             <  1:778443/148‑1 (MQ=255)
                   gcgcGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTg                                                                                                                             >  2:2109768/1‑148 (MQ=255)
                   gcgcGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTg                                                                                                                             <  1:2553590/148‑1 (MQ=255)
                    cgcgGTATTAATAAATGACTGTATTTGGGATTCGGTTTGCAAACGTTGCTGCAAGGTACTGTCTTCGGCTTTGCTGGCTATTGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGt                                                                                                                            <  1:2404498/148‑1 (MQ=255)
                         tagtaATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAg                                                                                                                       >  1:2260352/1‑148 (MQ=255)
                          agtaATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGGTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGt                                                                                                                      <  2:79911/148‑1 (MQ=255)
                             aataaATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCTAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGc                                                                                                                   <  2:2704846/148‑1 (MQ=255)
                               taaATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTg                                                                                                                 <  2:2153581/148‑1 (MQ=255)
                               taaATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTg                                                                                                                 <  1:1096204/148‑1 (MQ=255)
                                aaaTGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGt                                                                                                                <  1:3169663/148‑1 (MQ=255)
                                   tGACTGTAATTGGGCTTCGGTTTGCACACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTgg                                                                                                             <  1:2990849/148‑1 (MQ=255)
                                   tGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTgg                                                                                                             >  2:60435/1‑148 (MQ=255)
                                   tGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTgg                                                                                                             <  2:1881343/148‑1 (MQ=255)
                                   tGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTgg                                                                                                             <  1:2990838/148‑1 (MQ=255)
                                     aCTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTa                                                                                                           <  2:322705/148‑1 (MQ=255)
                                     aCTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTa                                                                                                           <  2:486794/148‑1 (MQ=255)
                                        gTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTg                                                                                                        <  2:2857381/148‑1 (MQ=255)
                                        gTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGATTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTg                                                                                                        >  2:386234/1‑148 (MQ=255)
                                        gTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGATTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTg                                                                                                        >  2:2182825/1‑148 (MQ=255)
                                             tGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTgcg                                                                                                   <  1:341705/148‑1 (MQ=255)
                                             tGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTgcg                                                                                                   >  2:308620/1‑148 (MQ=255)
                                                 cTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACg                                                                                               >  2:3347424/1‑148 (MQ=255)
                                                   tCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAg                                                                                             <  2:3449814/148‑1 (MQ=255)
                                                   tCGGTTTGCAAACGGTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAg                                                                                             <  1:1391799/148‑1 (MQ=255)
                                                    cGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGc                                                                                            <  1:893133/148‑1 (MQ=255)
                                                     ggTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGcc                                                                                           <  2:575594/148‑1 (MQ=255)
                                                       ttttCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTg                                                                                         <  1:1690237/148‑1 (MQ=255)
                                                       tttGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTg                                                                                         <  1:1773187/148‑1 (MQ=255)
                                                        ttGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGa                                                                                        <  2:3005575/148‑1 (MQ=255)
                                                           cAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCg                                                                                     <  1:2895549/148‑1 (MQ=255)
                                                             aaCGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCt                                                                                   >  1:1367453/1‑148 (MQ=255)
                                                              aCGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCtt                                                                                  <  1:1316406/148‑1 (MQ=255)
                                                               cGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTa                                                                                 <  2:1916684/148‑1 (MQ=255)
                                                                gTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTAc                                                                                <  1:2782753/148‑1 (MQ=255)
                                                                  tgctgcAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACgg                                                                              <  2:3298316/148‑1 (MQ=255)
                                                                  tgctgcAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACgg                                                                              <  1:1649686/148‑1 (MQ=255)
                                                                    ctgcAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGcc                                                                            >  2:1377873/1‑148 (MQ=255)
                                                                    ctgcAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGcc                                                                            <  2:2846864/148‑1 (MQ=255)
                                                                     tgcAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGccc                                                                           <  2:2260352/148‑1 (MQ=255)
                                                                     tgcAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGccc                                                                           <  1:363508/148‑1 (MQ=255)
                                                                       cAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCg                                                                         >  2:1696349/1‑148 (MQ=255)
                                                                        aaGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGa                                                                        <  2:3527830/148‑1 (MQ=255)
                                                                         aGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGAc                                                                       <  1:954063/148‑1 (MQ=255)
                                                                         aGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGAc                                                                       <  1:1520812/148‑1 (MQ=255)
                                                                          ggTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACt                                                                      >  2:259792/1‑148 (MQ=255)
                                                                          ggTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACt                                                                      >  2:3273931/1‑148 (MQ=255)
                                                                           gTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACtt                                                                     >  1:3223490/1‑148 (MQ=255)
                                                                             aCTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGc                                                                   >  1:391798/1‑148 (MQ=255)
                                                                                ggCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCAcc                                                                >  2:1729453/1‑148 (MQ=255)
                                                                                 gCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCa                                                               >  1:2054505/1‑148 (MQ=255)
                                                                                    tCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATaa                                                            <  1:2133304/148‑1 (MQ=255)
                                                                                     cGGCTTTGCTGGCGAATGGGCCGAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATaaa                                                           <  1:849273/148‑1 (MQ=255)
                                                                                     cGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATaaa                                                           <  2:242197/148‑1 (MQ=255)
                                                                                     cGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATaaa                                                           <  1:848869/148‑1 (MQ=255)
                                                                                     cGGCTTTGCTGGCGAATGGGCCAAGCTGGATCAGCCAGACCGCGCCATTTTGAGTTAAGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATaca                                                           >  2:378058/1‑146 (MQ=255)
                                                                                                    aTGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCt                                            <  2:397892/148‑1 (MQ=255)
                                                                                                     tGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCtt                                           <  2:2292665/148‑1 (MQ=255)
                                                                                                      gggCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCttt                                          <  1:2295241/148‑1 (MQ=255)
                                                                                                       ggCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTg                                         <  2:1262083/148‑1 (MQ=255)
                                                                                                       ggCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTg                                         <  2:726367/148‑1 (MQ=255)
                                                                                                       ggCCAAGCTGGATCCGCCAGACCGCGCCAATTTGAGTTACGATACCGCGGACGCCGAACTTCTGTCCCAGTTGCTCTTGGAACAGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGgcgcgtag                                         >  2:1709930/1‑144 (MQ=255)
                                                                                                         ccAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCAGAAAGTTGCCGCTGGCGCTTTGCg                                       >  2:532570/1‑148 (MQ=255)
                                                                                                           aaGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCgag                                     <  2:1077668/148‑1 (MQ=255)
                                                                                                           aaGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCgag                                     <  1:3431551/148‑1 (MQ=255)
                                                                                                            aGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCgaga                                    <  2:1790775/148‑1 (MQ=255)
                                                                                                            aGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCgaga                                    <  1:212513/148‑1 (MQ=255)
                                                                                                                ggATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGc                                >  1:1672261/1‑148 (MQ=255)
                                                                                                                ggATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGc                                <  1:460337/148‑1 (MQ=255)
                                                                                                                   tCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTg                             <  1:2008659/148‑1 (MQ=255)
                                                                                                                         aGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGgtgt                       >  2:747085/1‑148 (MQ=255)
                                                                                                                          gACCGCGCCATTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTc                      >  2:2447525/1‑148 (MQ=255)
                                                                                                                          gACCGCGCCATTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTc                      >  1:174631/1‑148 (MQ=255)
                                                                                                                             cgcgCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCAcc                   <  1:308620/148‑1 (MQ=255)
                                                                                                                                 ccATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTCCGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTg               <  1:386234/148‑1 (MQ=255)
                                                                                                                                 ccATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTg               <  1:2182825/148‑1 (MQ=255)
                                                                                                                                 ccATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTg               >  1:103145/1‑148 (MQ=255)
                                                                                                                                   aTTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCa             <  1:2059592/148‑1 (MQ=255)
                                                                                                                                   aTTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCa             >  1:1921162/1‑148 (MQ=255)
                                                                                                                                     tttGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAgg           >  1:58620/1‑148 (MQ=255)
                                                                                                                                        gAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGa        <  2:76895/148‑1 (MQ=255)
                                                                                                                                         aGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAg       <  1:2291521/148‑1 (MQ=255)
                                                                                                                                          gTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAgg      >  1:2846036/1‑148 (MQ=255)
                                                                                                                                           ttACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGt     >  1:1956494/1‑148 (MQ=255)
                                                                                                                                           ttACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGt     <  1:2536202/148‑1 (MQ=255)
                                                                                                                                              cgctACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGc  >  1:1050592/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGC  >  CP009273/659544‑659833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGG  >  CP009273/659542‑659836
                                                                                                                                                   |                                                                                                                                                   
AGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTAC                                                                                                                                                   >  SRR19973066.3153620/1‑150 (MQ=60)
 GATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACC                                                                                                                                                  <  SRR19973066.2617447/150‑1 (MQ=60)
 GATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACC                                                                                                                                                  >  SRR19973066.2742073/1‑150 (MQ=60)
 GATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACC                                                                                                                                                  <  SRR19973066.1325588/150‑1 (MQ=60)
  ATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCG                                                                                                                                                 <  SRR19973066.483546/150‑1 (MQ=60)
  ATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCG                                                                                                                                                 <  SRR19973066.2288792/150‑1 (MQ=60)
   TGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGG                                                                                                                                                >  SRR19973066.2221354/1‑150 (MQ=60)
    GCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGG                                                                                                                                               >  SRR19973066.2416776/1‑150 (MQ=60)
    GCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGG                                                                                                                                               >  SRR19973066.2416628/1‑150 (MQ=60)
       TGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGAC                                                                                                                                            >  SRR19973066.79488/1‑150 (MQ=60)
        GCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACG                                                                                                                                           <  SRR19973066.1394109/150‑1 (MQ=60)
         CTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGC                                                                                                                                          >  SRR19973066.395214/1‑150 (MQ=60)
           TTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCG                                                                                                                                        >  SRR19973066.2362352/1‑150 (MQ=60)
                CTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTT                                                                                                                                   <  SRR19973066.3080174/150‑1 (MQ=60)
                CTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTT                                                                                                                                   >  SRR19973066.1363714/1‑150 (MQ=60)
                     GCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTC                                                                                                                              <  SRR19973066.2570933/150‑1 (MQ=60)
                     GCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTC                                                                                                                              <  SRR19973066.784194/150‑1 (MQ=60)
                      CGCGGTATTAATAAATGACTGTATTTGGGATTCGGTTTGCAAACGTTGCTGCAAGGTACTGTCTTCGGCTTTGCTGGCTATTGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCC                                                                                                                             <  SRR19973066.2420756/150‑1 (MQ=60)
                         GGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCTAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAG                                                                                                                          >  SRR19973066.3490362/1‑150 (MQ=60)
                         GGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAG                                                                                                                          >  SRR19973066.470854/1‑150 (MQ=60)
                         GGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAG                                                                                                                          >  SRR19973066.2275920/1‑150 (MQ=60)
                            AGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCACCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTG                                                                                                                       <  SRR19973066.3088411/150‑1 (MQ=60)
                              TAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCT                                                                                                                     <  SRR19973066.1684755/150‑1 (MQ=60)
                               AATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTG                                                                                                                    >  SRR19973066.2308384/1‑150 (MQ=60)
                                 TAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTT                                                                                                                  >  SRR19973066.2852698/1‑150 (MQ=60)
                                 TAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTT                                                                                                                  <  SRR19973066.1104175/150‑1 (MQ=60)
                                  AAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTG                                                                                                                 <  SRR19973066.3190775/150‑1 (MQ=60)
                                  AAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTG                                                                                                                 >  SRR19973066.878201/1‑150 (MQ=60)
                                    ATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGT                                                                                                               >  SRR19973066.2778592/1‑150 (MQ=60)
                                    ATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGT                                                                                                               >  SRR19973066.1265631/1‑150 (MQ=60)
                                     TGACTGTAATTGGGCTTCGGTTTGCACACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTA                                                                                                              <  SRR19973066.3010652/150‑1 (MQ=60)
                                     TGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTA                                                                                                              <  SRR19973066.3010641/150‑1 (MQ=60)
                                       ACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACT                                                                                                            >  SRR19973066.1594079/1‑150 (MQ=60)
                                       ACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTACTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACT                                                                                                            >  SRR19973066.2128658/1‑150 (MQ=60)
                                          GTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTT                                                                                                         <  SRR19973066.3450296/150‑1 (MQ=60)
                                               TGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCA                                                                                                    <  SRR19973066.344345/150‑1 (MQ=60)
                                                     TCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCC                                                                                              <  SRR19973066.3186614/150‑1 (MQ=60)
                                                     TCGGTTTGCAAACGGTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCC                                                                                              <  SRR19973066.1401837/150‑1 (MQ=60)
                                                      CGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCT                                                                                             <  SRR19973066.899452/150‑1 (MQ=60)
                                                        GTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGA                                                                                           >  SRR19973066.957982/1‑150 (MQ=60)
                                                         TTTTCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGAT                                                                                          <  SRR19973066.1702536/150‑1 (MQ=60)
                                                         TTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGAT                                                                                          <  SRR19973066.1785875/150‑1 (MQ=60)
                                                             CAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCT                                                                                      <  SRR19973066.2914901/150‑1 (MQ=60)
                                                             CAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCT                                                                                      <  SRR19973066.974648/150‑1 (MQ=60)
                                                             CAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCT                                                                                      >  SRR19973066.1377344/1‑150 (MQ=60)
                                                              AAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTT                                                                                     >  SRR19973066.1508149/1‑150 (MQ=60)
                                                                ACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTAC                                                                                   <  SRR19973066.1325951/150‑1 (MQ=60)
                                                                  GTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGG                                                                                 <  SRR19973066.2801488/150‑1 (MQ=60)
                                                                    TGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCC                                                                               <  SRR19973066.1661728/150‑1 (MQ=60)
                                                                    TGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCC                                                                               <  SRR19973066.1661699/150‑1 (MQ=60)
                                                                       TGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCG                                                                            <  SRR19973066.2278082/150‑1 (MQ=60)
                                                                       TGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCG                                                                            <  SRR19973066.366359/150‑1 (MQ=60)
                                                                           AGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTT                                                                        <  SRR19973066.961036/150‑1 (MQ=60)
                                                                           AGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTT                                                                        >  SRR19973066.3244811/1‑150 (MQ=60)
                                                                           AGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTT                                                                        <  SRR19973066.1531539/150‑1 (MQ=60)
                                                                             GTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGC                                                                      >  SRR19973066.394887/1‑150 (MQ=60)
                                                                             GTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGC                                                                      >  SRR19973066.395464/1‑150 (MQ=60)
                                                                                 TGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCA                                                                  >  SRR19973066.2068766/1‑150 (MQ=60)
                                                                                 TGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCA                                                                  >  SRR19973066.2620163/1‑150 (MQ=60)
                                                                                  GGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCAT                                                                 >  SRR19973066.1386982/1‑150 (MQ=60)
                                                                                     TTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAA                                                              <  SRR19973066.2467033/150‑1 (MQ=60)
                                                                                      TCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAG                                                             <  SRR19973066.2148092/150‑1 (MQ=60)
                                                                                       CGGCTTTGCTGGCGAATGGGCCGAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGT                                                            <  SRR19973066.855422/150‑1 (MQ=60)
                                                                                       CGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGT                                                            <  SRR19973066.855016/150‑1 (MQ=60)
                                                                                           TTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCC                                                        <  SRR19973066.1088676/150‑1 (MQ=60)
                                                                                                        GGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGC                                           <  SRR19973066.2310977/150‑1 (MQ=60)
                                                                                                             AAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGAC                                      <  SRR19973066.3454509/150‑1 (MQ=60)
                                                                                                              AGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACG                                     <  SRR19973066.214319/150‑1 (MQ=60)
                                                                                                              AGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACG                                     <  SRR19973066.214271/150‑1 (MQ=60)
                                                                                                                CTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGC                                   >  SRR19973066.1684448/1‑150 (MQ=60)
                                                                                                                  GGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTT                                 <  SRR19973066.463860/150‑1 (MQ=60)
                                                                                                                  GGATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTT                                 <  SRR19973066.369007/150‑1 (MQ=60)
                                                                                                                   GATCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTG                                <  SRR19973066.2876696/150‑1 (MQ=60)
                                                                                                                   GATCCGCCAGACCGCGCCATTTTGAGTGACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTG                                <  SRR19973066.3146873/150‑1 (MQ=60)
                                                                                                                     TCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCG                              <  SRR19973066.543625/150‑1 (MQ=60)
                                                                                                                     TCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCG                              <  SRR19973066.2022743/150‑1 (MQ=60)
                                                                                                                     TCCGCCAGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCG                              <  SRR19973066.2022746/150‑1 (MQ=60)
                                                                                                                          CAGACCGCGCCATTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATCAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTC                         >  SRR19973066.176177/1‑150 (MQ=60)
                                                                                                                           AGACCGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCA                        <  SRR19973066.1708678/150‑1 (MQ=60)
                                                                                                                               CGCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCAT                    <  SRR19973066.310871/150‑1 (MQ=60)
                                                                                                                                GCGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATT                   <  SRR19973066.746554/150‑1 (MQ=60)
                                                                                                                                 CGCCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTG                  >  SRR19973066.104235/1‑150 (MQ=60)
                                                                                                                                   CCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCA                <  SRR19973066.389299/150‑1 (MQ=60)
                                                                                                                                   CCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCA                >  SRR19973066.1934824/1‑150 (MQ=60)
                                                                                                                                   CCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCA                <  SRR19973066.2197864/150‑1 (MQ=60)
                                                                                                                                   CCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCA                <  SRR19973066.2197975/150‑1 (MQ=60)
                                                                                                                                   CCATTTTGAGTTACGCTACCGGGGACGCCGAACTTCGGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTCCGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCA                <  SRR19973066.389293/150‑1 (MQ=60)
                                                                                                                                     ATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGG              <  SRR19973066.2073876/150‑1 (MQ=60)
                                                                                                                                     ATTTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGG              >  SRR19973066.59348/1‑150 (MQ=60)
                                                                                                                                       TTTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCG            >  SRR19973066.2962155/1‑150 (MQ=60)
                                                                                                                                        TTGAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCNTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGA           <  SRR19973066.1819145/150‑1 (MQ=60)
                                                                                                                                          GAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGG         >  SRR19973066.2865158/1‑150 (MQ=60)
                                                                                                                                          GAGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGG         >  SRR19973066.3045619/1‑150 (MQ=60)
                                                                                                                                           AGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGT        <  SRR19973066.2307232/150‑1 (MQ=60)
                                                                                                                                           AGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGT        <  SRR19973066.3369711/150‑1 (MQ=60)
                                                                                                                                           AGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGT        >  SRR19973066.1969669/1‑150 (MQ=60)
                                                                                                                                           AGTTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGT        >  SRR19973066.1970337/1‑150 (MQ=60)
                                                                                                                                             TTACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTG      <  SRR19973066.2553440/150‑1 (MQ=60)
                                                                                                                                              TACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGC     >  SRR19973066.1058317/1‑150 (MQ=60)
                                                                                                                                               ACGCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCA    <  SRR19973066.1990460/150‑1 (MQ=60)
                                                                                                                                                 GCTACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGG  >  SRR19973066.3138033/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                   
AGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCGACCGGGGACGCCGAACTTCTGTCCCAGTTGCTGTTGGTACTGTTGCGCACGAGCCTGATCGCTTACGGCCCCGACTTGCACCATAAAGTTGCCGCTGGCGCTTTGCGAGACGGCTTGCGGTGTCACCATTGCAGGCGAGGTTGCAGG  >  CP009273/659542‑659836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑