Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 680,846 G→T 90.9% F180L (TTC→TTA)  gltK ← glutamate, aspartate ABC transporter permease subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273680,8460GT90.9% 525.0 / 45.9 198F180L (TTC→TTA) gltKglutamate, aspartate ABC transporter permease subunit
Reads supporting (aligned to +/- strand):  ref base G (6/12);  new base T (104/76);  total (110/88)
Fisher's exact test for biased strand distribution p-value = 7.92e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 205 total aligned reads displayed.

AGGGTAATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGC  >  CP009273/680701‑680993
                                                                                                                                                 |                                                                                                                                                   
aGGGTAATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGgaataa                                                                                                                                                   <  2:2092521/148‑1 (MQ=255)
aGGGTAATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGgaataa                                                                                                                                                   <  1:2622056/148‑1 (MQ=255)
 gggTAATCATTATGCTGTCCTTCATTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGaaa                                                                                                                                                  <  2:2609634/148‑1 (MQ=255)
     aaTCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGATAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCgg                                                                                                                                              >  2:655326/1‑148 (MQ=255)
      ataaTTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGc                                                                                                                                             <  1:2235967/145‑1 (MQ=255)
      aTCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGc                                                                                                                                             <  1:1745928/148‑1 (MQ=255)
       tCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGcc                                                                                                                                            >  1:294471/1‑148 (MQ=255)
        cATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCa                                                                                                                                           >  2:1252759/1‑148 (MQ=255)
        aaTTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCa                                                                                                                                           <  2:718330/147‑1 (MQ=255)
         aTTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAg                                                                                                                                          <  2:3181616/148‑1 (MQ=255)
         aTTATGCTGTCATTATTTTCAAGTAGCTCACAAACAACGACGCGCTAATACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCAACACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAg                                                                                                                                          <  1:886729/148‑1 (MQ=255)
              ggtttctttcttTTCAATTAGCTTACAAAAAACGACGCGATAAGACTAATAACTAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATa                                                                                                                                     <  1:2858678/141‑1 (MQ=255)
              gCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAAGGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATa                                                                                                                                     <  2:941815/148‑1 (MQ=255)
                tGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAac                                                                                                                                   >  2:2946422/1‑148 (MQ=255)
                tGTCCTTCTTTTCAAGTAGATGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGTTTGAGGCGGTAAGGAATAAATCGGCCAGGGATAac                                                                                                                                   >  2:1505006/1‑148 (MQ=255)
                      tcttTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATac                                                                                                                             <  2:900322/148‑1 (MQ=255)
                      tcttTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATac                                                                                                                             <  2:2894865/148‑1 (MQ=255)
                       cttTTAAAGTAGCTCACAAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGTAATAAATCGGCCAGGGATAACACATaca                                                                                                                            <  1:1574566/148‑1 (MQ=255)
                             aaGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTg                                                                                                                      >  2:2572493/1‑148 (MQ=255)
                              aGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGa                                                                                                                     >  2:675710/1‑148 (MQ=255)
                               gTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAg                                                                                                                    <  2:1933212/148‑1 (MQ=255)
                               gTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAg                                                                                                                    >  2:3018654/1‑148 (MQ=255)
                                 aGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGt                                                                                                                  <  2:282786/148‑1 (MQ=255)
                                  gCTGACCAACCACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTa                                                                                                                 <  2:2524444/148‑1 (MQ=255)
                                  gCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTa                                                                                                                 >  2:3224106/1‑148 (MQ=255)
                                  gCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTa                                                                                                                 >  1:1217529/1‑148 (MQ=255)
                                        caacaaCGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTgg                                                                                                           >  2:2249538/1‑148 (MQ=255)
                                          acaacGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGaa                                                                                                         <  1:1722421/148‑1 (MQ=255)
                                          acaacGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGaa                                                                                                         <  1:1252759/148‑1 (MQ=255)
                                            aacGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACa                                                                                                       <  2:2151143/148‑1 (MQ=255)
                                              cgacgCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGt                                                                                                     >  1:2694631/1‑148 (MQ=255)
                                                 cgcgCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACg                                                                                                  >  2:1644798/1‑148 (MQ=255)
                                                     cTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGc                                                                                              >  1:1453568/1‑148 (MQ=255)
                                                     cTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGAAAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGc                                                                                              >  1:3096164/1‑148 (MQ=255)
                                                      tAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGcc                                                                                             >  2:2279362/1‑148 (MQ=255)
                                                           cTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCtgag                                                                                        >  1:2545359/1‑148 (MQ=255)
                                                            taataaCGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCtgagt                                                                                       >  2:247200/1‑148 (MQ=255)
                                                             aataaCGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCtgagtg                                                                                      >  2:798102/1‑148 (MQ=255)
                                                              ataaCGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCGGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCtgagtga                                                                                     >  2:2814483/1‑148 (MQ=255)
                                                               taaCGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCtgagtgag                                                                                    >  2:321421/1‑148 (MQ=255)
                                                                aaCGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGagc                                                                                   >  2:1555079/1‑148 (MQ=255)
                                                                aaCGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGagc                                                                                   >  1:644084/1‑148 (MQ=255)
                                                                 aCGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGagca                                                                                  <  1:710988/148‑1 (MQ=255)
                                                                 aCGAAATAAACAAATCCGGAAAACAGGATCATCTCAACCTGCTTACCAACACGCTCAACAATGTTTGAGGCGGTACGTAATAAATCGGCCAGTGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGagca                                                                                  <  1:710961/148‑1 (MQ=255)
                                                                  cGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGagcag                                                                                 >  1:2146879/1‑148 (MQ=255)
                                                                  cGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGagcag                                                                                 >  1:485052/1‑148 (MQ=255)
                                                                    aaaTAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGagcagca                                                                               >  1:1581119/1‑148 (MQ=255)
                                                                     aaTAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGagcagcag                                                                              >  1:1360235/1‑148 (MQ=255)
                                                                     aaTAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGagcagcag                                                                              >  1:2799250/1‑148 (MQ=255)
                                                                     aaTAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGagcagcag                                                                              >  1:1145703/1‑148 (MQ=255)
                                                                      aTAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGagcagcagc                                                                             >  1:1066602/1‑148 (MQ=255)
                                                                      aTAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGagcagcagc                                                                             >  2:1172106/1‑148 (MQ=255)
                                                                       tAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCg                                                                            >  2:1085886/1‑148 (MQ=255)
                                                                        aaaCAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCgg                                                                           >  1:451252/1‑148 (MQ=255)
                                                                           cAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCAc                                                                        >  2:2120394/1‑148 (MQ=255)
                                                                            aaaTCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCAcc                                                                       >  1:1036584/1‑148 (MQ=255)
                                                                             aaTCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCa                                                                      >  1:2759665/1‑148 (MQ=255)
                                                                              aTCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCAt                                                                     >  2:1816811/1‑148 (MQ=255)
                                                                                 cGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATcgc                                                                  >  1:627726/1‑148 (MQ=255)
                                                                                       aCAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGaa                                                            >  1:833724/1‑148 (MQ=255)
                                                                                       aCAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGaa                                                            >  1:2833757/1‑148 (MQ=255)
                                                                                          ggATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGc                                                         <  1:52928/148‑1 (MQ=255)
                                                                                              catcTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGc                                                     >  1:2820576/1‑148 (MQ=255)
                                                                                                tctcAACCTGCGTACCATCACGCTCACCACTGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCgg                                                   <  1:210537/148‑1 (MQ=255)
                                                                                                 ctcAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGc                                                  >  1:916439/1‑148 (MQ=255)
                                                                                                    aaCCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGa                                               <  2:3270549/148‑1 (MQ=255)
                                                                                                     aCCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGaa                                              <  2:1860465/148‑1 (MQ=255)
                                                                                                       cTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGaata                                            >  1:2698024/1‑148 (MQ=255)
                                                                                                       cTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGaata                                            >  1:99140/1‑148 (MQ=255)
                                                                                                       cTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGaata                                            >  1:2912010/1‑148 (MQ=255)
                                                                                                         gCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGaataat                                          >  1:2318739/1‑148 (MQ=255)
                                                                                                         gCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGaataat                                          <  2:385393/148‑1 (MQ=255)
                                                                                                          cGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATc                                         <  1:3262322/148‑1 (MQ=255)
                                                                                                           gTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCa                                        <  1:1802881/148‑1 (MQ=255)
                                                                                                            tACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAg                                       <  1:1765292/148‑1 (MQ=255)
                                                                                                              ccATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGtt                                     >  1:2409210/1‑148 (MQ=255)
                                                                                                              ccATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGtt                                     >  1:1411841/1‑148 (MQ=255)
                                                                                                              ccATCACGCTCACAAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGtt                                     >  2:1241419/1‑148 (MQ=255)
                                                                                                               cATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGttt                                    <  1:2996659/148‑1 (MQ=255)
                                                                                                               cATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGttt                                    >  2:2749759/1‑148 (MQ=255)
                                                                                                               cATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGttt                                    <  1:2690422/148‑1 (MQ=255)
                                                                                                                 tCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCa                                  >  2:1685812/1‑148 (MQ=255)
                                                                                                                 tCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCa                                  <  2:1445799/148‑1 (MQ=255)
                                                                                                                  cACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCAt                                 >  2:1330727/1‑148 (MQ=255)
                                                                                                                     gCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGa                              <  1:1628511/148‑1 (MQ=255)
                                                                                                                           cAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCa                        >  2:2000960/1‑148 (MQ=255)
                                                                                                                             aTGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGt                      <  2:1894805/148‑1 (MQ=255)
                                                                                                                              tGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTg                     <  2:2759665/148‑1 (MQ=255)
                                                                                                                                gTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAg                   <  2:1036584/148‑1 (MQ=255)
                                                                                                                                   gAGGCGGTACGTAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCa                <  1:1171110/148‑1 (MQ=255)
                                                                                                                                   gAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCa                <  2:2694490/148‑1 (MQ=255)
                                                                                                                                   gAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCa                <  2:1597243/148‑1 (MQ=255)
                                                                                                                                     ggCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCAtt              >  1:1626040/1‑148 (MQ=255)
                                                                                                                                     ggCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCAtt              <  2:2355233/148‑1 (MQ=255)
                                                                                                                                     ggCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCAtt              >  1:2345494/1‑148 (MQ=255)
                                                                                                                                          tACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCaa         <  2:1144396/148‑1 (MQ=255)
                                                                                                                                             ggAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGc      <  1:2000108/148‑1 (MQ=255)
                                                                                                                                              gaataaATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGcc     >  1:1385666/1‑148 (MQ=255)
                                                                                                                                                 gaaATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAgc  >  2:1133282/1‑148 (MQ=255)
                                                                                                                                                 gaaATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAgc  >  2:1133219/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
AGGGTAATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGC  >  CP009273/680701‑680993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGGGTAATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGC  >  CP009273/680701‑680995
                                                                                                                                                 |                                                                                                                                                     
AGGGTAATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAAT                                                                                                                                                   <  SRR19973070.2639160/150‑1 (MQ=60)
  GGTAATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCG                                                                                                                                                 <  SRR19973070.2949040/150‑1 (MQ=60)
   GTAATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGG                                                                                                                                                <  SRR19973070.2622683/150‑1 (MQ=60)
     AATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCC                                                                                                                                              >  SRR19973070.491869/1‑150 (MQ=60)
     AATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCC                                                                                                                                              >  SRR19973070.1974634/1‑150 (MQ=60)
     AATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCC                                                                                                                                              >  SRR19973070.296499/1‑150 (MQ=60)
      ATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCA                                                                                                                                             >  SRR19973070.2748051/1‑150 (MQ=60)
      ATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCA                                                                                                                                             >  SRR19973070.1790581/1‑150 (MQ=60)
      ATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCA                                                                                                                                             <  SRR19973070.1758037/150‑1 (MQ=60)
      ATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCA                                                                                                                                             <  SRR19973070.1758022/150‑1 (MQ=60)
      ATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCA                                                                                                                                             <  SRR19973070.1758039/150‑1 (MQ=60)
      ATAATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCA                                                                                                                                             <  SRR19973070.2250709/150‑1 (MQ=60)
       TCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAG                                                                                                                                            <  SRR19973070.2702786/150‑1 (MQ=60)
         ATTATGCTGTCATTATTTTCAAGTAGCTCACAAACAACGACGCGCTAATACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCAACACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGG                                                                                                                                          <  SRR19973070.892989/150‑1 (MQ=60)
              ggtttctTTCTTTTCAATTAGCTTACAAAAAACGACGCGATAAGACTAATAACTAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAAC                                                                                                                                     <  SRR19973070.2877202/143‑1 (MQ=60)
              GCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAAC                                                                                                                                     <  SRR19973070.2877196/150‑1 (MQ=60)
                TGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACAC                                                                                                                                   <  SRR19973070.1577147/150‑1 (MQ=60)
                TGTCCTTATTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACAC                                                                                                                                   <  SRR19973070.649685/150‑1 (MQ=60)
                      TCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACAC                                                                                                                             >  SRR19973070.2970012/1‑150 (MQ=60)
                       CTTTTCACGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACC                                                                                                                            <  SRR19973070.1585675/150‑1 (MQ=60)
                       CTTTTAAAGTAGCTCACAAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGTAATAAATCGGCCAGGGATAACACATACACC                                                                                                                            <  SRR19973070.1585617/150‑1 (MQ=60)
                                TAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTA                                                                                                                   >  SRR19973070.2911762/1‑150 (MQ=60)
                                TAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTA                                                                                                                   >  SRR19973070.1226063/1‑150 (MQ=60)
                                TAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACAAATGGTTGAGGCGGTACGGAATAAATCTGCCAGGGATAACACATACACCAGTGAGGTA                                                                                                                   >  SRR19973070.379189/1‑150 (MQ=60)
                                    TGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCT                                                                                                               >  SRR19973070.813773/1‑150 (MQ=60)
                                          ACCACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACA                                                                                                         <  SRR19973070.1259771/150‑1 (MQ=60)
                                          ACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACA                                                                                                         <  SRR19973070.1261545/150‑1 (MQ=60)
                                          ACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACA                                                                                                         <  SRR19973070.1734345/150‑1 (MQ=60)
                                            AACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGT                                                                                                       >  SRR19973070.2712084/1‑150 (MQ=60)
                                            AACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGT                                                                                                       >  SRR19973070.2711943/1‑150 (MQ=60)
                                              CGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTAC                                                                                                     <  SRR19973070.2155079/150‑1 (MQ=60)
                                              CGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTAC                                                                                                     <  SRR19973070.2155039/150‑1 (MQ=60)
                                                   CGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGC                                                                                                >  SRR19973070.1463688/1‑150 (MQ=60)
                                                   CGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGC                                                                                                <  SRR19973070.400349/150‑1 (MQ=60)
                                                   CGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGAAAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGC                                                                                                >  SRR19973070.3116113/1‑150 (MQ=60)
                                                      TAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCT                                                                                             >  SRR19973070.3214759/1‑150 (MQ=60)
                                                         GACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAG                                                                                          >  SRR19973070.2561998/1‑150 (MQ=60)
                                                           CTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTG                                                                                        >  SRR19973070.647382/1‑150 (MQ=60)
                                                            TAATACCCAAACAAACAACTCCGGCACACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAAGGGTTGAGGCGGTACGTAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGA                                                                                       <  SRR19973070.971689/150‑1 (MQ=60)
                                                            TAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGA                                                                                       <  SRR19973070.971798/150‑1 (MQ=60)
                                                             AATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAG                                                                                      <  SRR19973070.2344190/150‑1 (MQ=60)
                                                              ATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGC                                                                                     >  SRR19973070.648679/1‑150 (MQ=60)
                                                                AACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAG                                                                                   >  SRR19973070.2161187/1‑150 (MQ=60)
                                                                AACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAG                                                                                   >  SRR19973070.488466/1‑150 (MQ=60)
                                                                 ACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGC                                                                                  <  SRR19973070.716045/150‑1 (MQ=60)
                                                                 ACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGC                                                                                  <  SRR19973070.716059/150‑1 (MQ=60)
                                                                 ACGAAATAAACAAATCCGGAAAACAGGATCATCTCAACCTGCTTACCAACACGCTCAACAATGTTTGAGGCGGTACGTAATAAATCGGCCAGTGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGC                                                                                  <  SRR19973070.716032/150‑1 (MQ=60)
                                                                  CGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCA                                                                                 >  SRR19973070.1592203/1‑150 (MQ=60)
                                                                   GAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAG                                                                                >  SRR19973070.1369511/1‑150 (MQ=60)
                                                                   GAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAG                                                                                >  SRR19973070.1153665/1‑150 (MQ=60)
                                                                   GAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAG                                                                                >  SRR19973070.2817327/1‑150 (MQ=60)
                                                                    AAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGC                                                                               >  SRR19973070.1074022/1‑150 (MQ=60)
                                                                    AAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGC                                                                               >  SRR19973070.1074069/1‑150 (MQ=60)
                                                                    AAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGC                                                                               >  SRR19973070.1075259/1‑150 (MQ=60)
                                                                      ATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGG                                                                             >  SRR19973070.454652/1‑150 (MQ=60)
                                                                      ATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGG                                                                             >  SRR19973070.454504/1‑150 (MQ=60)
                                                                       TAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGC                                                                            >  SRR19973070.2822422/1‑150 (MQ=60)
                                                                         AACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCAC                                                                          >  SRR19973070.209714/1‑150 (MQ=60)
                                                                          ACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACC                                                                         >  SRR19973070.1043907/1‑150 (MQ=60)
                                                                           CAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCA                                                                        >  SRR19973070.2777366/1‑150 (MQ=60)
                                                                             AATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATC                                                                      >  SRR19973070.1616794/1‑150 (MQ=60)
                                                                               TCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGC                                                                    >  SRR19973070.632169/1‑150 (MQ=60)
                                                                               TCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGC                                                                    >  SRR19973070.2305396/1‑150 (MQ=60)
                                                                                     AAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAA                                                              >  SRR19973070.839376/1‑150 (MQ=60)
                                                                                     AAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAA                                                              >  SRR19973070.1419889/1‑150 (MQ=60)
                                                                                     AAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAA                                                              >  SRR19973070.839369/1‑150 (MQ=60)
                                                                                     AAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAA                                                              >  SRR19973070.2852096/1‑150 (MQ=60)
                                                                                      AACAGGATCATCCCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAAC                                                             <  SRR19973070.1182312/150‑1 (MQ=60)
                                                                                          GGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCT                                                         <  SRR19973070.53564/150‑1 (MQ=60)
                                                                                            ATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGC                                                       >  SRR19973070.2210219/1‑150 (MQ=60)
                                                                                            ATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGC                                                       >  SRR19973070.2838813/1‑150 (MQ=60)
                                                                                               ATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGC                                                    >  SRR19973070.922962/1‑150 (MQ=60)
                                                                                                TCTCAACCTGCGTACCATCACGCTCACCACTGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCA                                                   <  SRR19973070.212096/150‑1 (MQ=60)
                                                                                                    AACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAAT                                               <  SRR19973070.1175705/150‑1 (MQ=60)
                                                                                                     ACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATA                                              >  SRR19973070.100099/1‑150 (MQ=60)
                                                                                                     ACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATA                                              >  SRR19973070.3084337/1‑150 (MQ=60)
                                                                                                     ACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATA                                              >  SRR19973070.2930833/1‑150 (MQ=60)
                                                                                                     ACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATA                                              >  SRR19973070.2715324/1‑150 (MQ=60)
                                                                                                     ACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATA                                              >  SRR19973070.2715486/1‑150 (MQ=60)
                                                                                                       CTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAAT                                            >  SRR19973070.2334148/1‑150 (MQ=60)
                                                                                                         GCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCA                                          <  SRR19973070.2365741/150‑1 (MQ=60)
                                                                                                          CGTACCATCACGCTCACCAATGGTTGAGGCGTTACGTAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAG                                         <  SRR19973070.2496587/150‑1 (MQ=60)
                                                                                                          CGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAG                                         <  SRR19973070.3283319/150‑1 (MQ=60)
                                                                                                           GTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGT                                        <  SRR19973070.1815328/150‑1 (MQ=60)
                                                                                                            TACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTT                                       >  SRR19973070.1421479/1‑150 (MQ=60)
                                                                                                            TACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTT                                       >  SRR19973070.2425091/1‑150 (MQ=60)
                                                                                                            TACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTT                                       <  SRR19973070.1777502/150‑1 (MQ=60)
                                                                                                               CATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCA                                    <  SRR19973070.3015843/150‑1 (MQ=60)
                                                                                                               CATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCA                                    <  SRR19973070.2707852/150‑1 (MQ=60)
                                                                                                               CATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCA                                    <  SRR19973070.1375589/150‑1 (MQ=60)
                                                                                                                    CGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGAC                               >  SRR19973070.912031/1‑150 (MQ=60)
                                                                                                                     GCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACT                              <  SRR19973070.1639813/150‑1 (MQ=60)
                                                                                                                     GCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACT                              <  SRR19973070.1639818/150‑1 (MQ=60)
                                                                                                                         ACCATGGGTTGAGGCGTTACGTAATAAATCGGCCAGGGATAACACATACACCAGTGTGGTATCCTGTAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCA                          <  SRR19973070.1432228/150‑1 (MQ=60)
                                                                                                                             TTGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGA                      <  SRR19973070.2803945/150‑1 (MQ=60)
                                                                                                                                   GAGGCGGTACGTAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATT                <  SRR19973070.1179310/150‑1 (MQ=60)
                                                                                                                                   GAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGTCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATT                <  SRR19973070.2840830/150‑1 (MQ=60)
                                                                                                                                   GAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATT                >  SRR19973070.1637333/1‑150 (MQ=60)
                                                                                                                                   GAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATT                >  SRR19973070.1637336/1‑150 (MQ=60)
                                                                                                                                   GAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATT                >  SRR19973070.2361051/1‑150 (MQ=60)
                                                                                                                                        GGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAA           >  SRR19973070.1349806/1‑150 (MQ=60)
                                                                                                                                         GTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAAC          <  SRR19973070.626836/150‑1 (MQ=60)
                                                                                                                                            CGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCC       >  SRR19973070.1395040/1‑150 (MQ=60)
                                                                                                                                             GGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCA      <  SRR19973070.2013654/150‑1 (MQ=60)
                                                                                                                                             GGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCA      >  SRR19973070.511289/1‑150 (MQ=60)
                                                                                                                                                ATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCG   <  SRR19973070.854970/150‑1 (MQ=60)
                                                                                                                                                 TAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGC  >  SRR19973070.1425102/1‑150 (MQ=60)
                                                                                                                                                 |                                                                                                                                                     
AGGGTAATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGC  >  CP009273/680701‑680995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑