Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 680,846 G→T 100% F180L (TTC→TTA)  gltK ← glutamate, aspartate ABC transporter permease subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273680,8460GT99.4% 605.8 / ‑4.2 187F180L (TTC→TTA) gltKglutamate, aspartate ABC transporter permease subunit
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base T (103/82);  total (103/84)
Fisher's exact test for biased strand distribution p-value = 2.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 191 total aligned reads displayed.

AGGGTAATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCC  >  CP009273/680701‑680990
                                                                                                                                                 |                                                                                                                                                
 gggTAATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATaaa                                                                                                                                               <  1:2034292/148‑1 (MQ=255)
  ggTAATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAAt                                                                                                                                              >  2:3397221/1‑148 (MQ=255)
     aaTCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCgg                                                                                                                                           >  2:2562247/1‑148 (MQ=255)
      aTCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGc                                                                                                                                          <  1:3359727/148‑1 (MQ=255)
      aTCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGc                                                                                                                                          <  1:1592057/148‑1 (MQ=255)
      aTCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGc                                                                                                                                          <  2:1087232/148‑1 (MQ=255)
       tCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGcc                                                                                                                                         >  2:2307522/1‑148 (MQ=255)
        cATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCa                                                                                                                                        <  2:1428652/148‑1 (MQ=255)
        cATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCa                                                                                                                                        >  1:2325628/1‑148 (MQ=255)
         tttATGCTGTCCTTCTTTTCATGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAg                                                                                                                                       <  1:1890912/147‑1 (MQ=255)
         tttATGCTGTCCTTCTTTTCAAGTCCCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCACCCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAg                                                                                                                                       <  1:3047573/147‑1 (MQ=255)
            aTGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGa                                                                                                                                    >  1:102225/1‑148 (MQ=255)
            aTGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGa                                                                                                                                    <  1:439480/148‑1 (MQ=255)
             tGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGAt                                                                                                                                   >  2:2346391/1‑148 (MQ=255)
             tGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCAGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGAt                                                                                                                                   >  2:1203379/1‑148 (MQ=255)
              gCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATa                                                                                                                                  <  1:1492416/148‑1 (MQ=255)
                  tCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAacac                                                                                                                              >  2:936699/1‑148 (MQ=255)
                  tCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAacac                                                                                                                              <  1:2533190/148‑1 (MQ=255)
                  tCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAacac                                                                                                                              <  1:1729968/148‑1 (MQ=255)
                  tCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAacac                                                                                                                              <  1:1730056/148‑1 (MQ=255)
                   ccTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAAcaca                                                                                                                             >  1:1801742/1‑148 (MQ=255)
                     ttcttTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATa                                                                                                                           >  2:2941092/1‑148 (MQ=255)
                      tcttTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATac                                                                                                                          <  1:3464078/148‑1 (MQ=255)
                       cttTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATgca                                                                                                                         >  1:734503/1‑148 (MQ=255)
                       cttTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATaca                                                                                                                         >  2:2724581/1‑148 (MQ=255)
                        ttttCAAGTAGCTGACAAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATacac                                                                                                                        <  1:2218762/148‑1 (MQ=255)
                         tttCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACAcc                                                                                                                       >  2:2314754/1‑148 (MQ=255)
                         tttCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACAcc                                                                                                                       <  2:1790914/148‑1 (MQ=255)
                              aGTAGCTGACCAACAACGACGCTCTAAGACTAATAACGAAATAATCAAATCCGGCATACAGGATCATCTCAACCTGCGTACCATCACTCTCACCAATGGTTGAGGCGGTACGGAATAAATCGTCCAGGGATAACACATACACCAGTGa                                                                                                                  <  2:1965024/148‑1 (MQ=255)
                              aGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCTCGCTCACCTATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGa                                                                                                                  <  2:2068928/148‑1 (MQ=255)
                                tAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAgg                                                                                                                >  1:642285/1‑148 (MQ=255)
                                  gCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTa                                                                                                              <  2:1563871/148‑1 (MQ=255)
                                   atGACCAAAAACGACGCTCTAAGAATAATAACGAAATAAACAAATCAGGCAAACAGGATCATCTCACCCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGTAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTAt                                                                                                             <  1:2494381/147‑1 (MQ=255)
                                     gACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATcc                                                                                                           >  1:2064312/1‑148 (MQ=255)
                                        caacaaCGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTgg                                                                                                        <  1:1487106/148‑1 (MQ=255)
                                        caacaaCGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTgg                                                                                                        <  1:3258921/148‑1 (MQ=255)
                                         aacaacGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGa                                                                                                       <  2:3051222/148‑1 (MQ=255)
                                         aacaacGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGa                                                                                                       >  2:2187881/1‑148 (MQ=255)
                                          acaacGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGaa                                                                                                      >  1:1442045/1‑148 (MQ=255)
                                           caacGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAAc                                                                                                     <  2:2334227/148‑1 (MQ=255)
                                                 cgcgCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACg                                                                                               >  1:574386/1‑148 (MQ=255)
                                                  gcgcTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGa                                                                                              <  2:3557833/148‑1 (MQ=255)
                                                  gcgcTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGa                                                                                              >  1:945563/1‑148 (MQ=255)
                                                      tAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGcc                                                                                          >  1:167040/1‑148 (MQ=255)
                                                      tAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGcc                                                                                          >  2:1951981/1‑148 (MQ=255)
                                                      tAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGcc                                                                                          <  2:1446525/148‑1 (MQ=255)
                                                        aGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCt                                                                                        >  2:598582/1‑148 (MQ=255)
                                                          aCTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGACCAGTACGAGGCCCtga                                                                                      >  1:2861766/1‑148 (MQ=255)
                                                          aCTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCtga                                                                                      >  1:1675830/1‑148 (MQ=255)
                                                            taataaCGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCtgagt                                                                                    >  2:3243867/1‑148 (MQ=255)
                                                            taataaCGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCtgagt                                                                                    >  2:3243934/1‑148 (MQ=255)
                                                              ataaCGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCtgagtga                                                                                  >  1:3240111/1‑148 (MQ=255)
                                                               taaCGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCtgagtgag                                                                                 <  2:2563089/148‑1 (MQ=255)
                                                               taaCGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCtgagtgag                                                                                 <  1:1620180/148‑1 (MQ=255)
                                                                aaCGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGagc                                                                                >  2:2877295/1‑148 (MQ=255)
                                                                  cGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGagcag                                                                              >  1:3505269/1‑148 (MQ=255)
                                                                   gAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGagcagc                                                                             >  2:166149/1‑148 (MQ=255)
                                                                      aTAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGagcagcagc                                                                          >  2:3128926/1‑148 (MQ=255)
                                                                          aCAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCa                                                                      >  1:2934671/1‑148 (MQ=255)
                                                                           cAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCAc                                                                     <  1:196698/148‑1 (MQ=255)
                                                                           cAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCAc                                                                     <  2:2890304/148‑1 (MQ=255)
                                                                            aaaTCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCAcc                                                                    <  2:69586/148‑1 (MQ=255)
                                                                              aTCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCAt                                                                  >  2:3189154/1‑148 (MQ=255)
                                                                              aTCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCAt                                                                  >  1:269302/1‑148 (MQ=255)
                                                                              aTCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCAt                                                                  >  2:3188851/1‑148 (MQ=255)
                                                                                ccGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATcg                                                                <  1:817977/148‑1 (MQ=255)
                                                                                       acacGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGaa                                                         <  1:3397221/148‑1 (MQ=255)
                                                                                       aCAGGATCATCTCACCCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGaa                                                         <  1:3113879/148‑1 (MQ=255)
                                                                                       aCAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGaa                                                         >  2:2006212/1‑148 (MQ=255)
                                                                                          ggATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGc                                                      <  1:3536149/148‑1 (MQ=255)
                                                                                           gATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGcc                                                     >  2:130008/1‑148 (MQ=255)
                                                                                            atcatcTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCt                                                    <  2:2492123/148‑1 (MQ=255)
                                                                                             tcatcTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATTCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTg                                                   >  1:3149115/1‑148 (MQ=255)
                                                                                              catcTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGc                                                  >  2:2651345/1‑148 (MQ=255)
                                                                                              catcTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGc                                                  >  2:864423/1‑148 (MQ=255)
                                                                                                tctcAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCgg                                                >  1:2887874/1‑148 (MQ=255)
                                                                                                 ctcAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGc                                               >  1:2353196/1‑148 (MQ=255)
                                                                                                  tcAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCa                                              >  1:1959933/1‑148 (MQ=255)
                                                                                                    aaCCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGa                                            >  2:1038269/1‑148 (MQ=255)
                                                                                                    aaCCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGa                                            <  1:3128926/148‑1 (MQ=255)
                                                                                                     aCCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGaa                                           <  2:642364/148‑1 (MQ=255)
                                                                                                     aCCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGaa                                           <  2:3455451/148‑1 (MQ=255)
                                                                                                          cGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATc                                      <  1:2096646/148‑1 (MQ=255)
                                                                                                           gTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCa                                     >  2:2071322/1‑148 (MQ=255)
                                                                                                             aCCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGt                                   <  1:658162/148‑1 (MQ=255)
                                                                                                             aCCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGt                                   <  1:1082676/148‑1 (MQ=255)
                                                                                                              ccATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGtt                                  >  2:2122532/1‑148 (MQ=255)
                                                                                                                 tCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCa                               <  1:2387609/148‑1 (MQ=255)
                                                                                                                 tCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCa                               >  1:1173535/1‑148 (MQ=255)
                                                                                                                   aCGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATc                             >  1:1132285/1‑148 (MQ=255)
                                                                                                                    cGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCg                            <  1:2622178/148‑1 (MQ=255)
                                                                                                                    cGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCg                            >  2:3215394/1‑148 (MQ=255)
                                                                                                                      cTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGAc                          >  2:2527712/1‑148 (MQ=255)
                                                                                                                         aCCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGc                       >  2:9072/1‑148 (MQ=255)
                                                                                                                            aaTGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAg                    <  1:2786081/148‑1 (MQ=255)
                                                                                                                            aaTGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAg                    >  1:457662/1‑148 (MQ=255)
                                                                                                                                gTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAg                >  2:1920221/1‑148 (MQ=255)
                                                                                                                                         gTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCa       <  2:818274/148‑1 (MQ=255)
                                                                                                                                             tgaataaATCGGCCAGGTATAACACATACACCAGTGTGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGc   <  1:92185/147‑1 (MQ=255)
                                                                                                                                              gaataaATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGcc  >  1:3166384/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AGGGTAATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCC  >  CP009273/680701‑680990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTCAGGGTAATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAG  >  CP009273/680698‑680992
                                                                                                                                                    |                                                                                                                                                  
TTCAGGGTAATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATA                                                                                                                                                   >  SRR19973066.2027326/1‑150 (MQ=60)
TTCAGGGTAATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATA                                                                                                                                                   >  SRR19973066.306703/1‑150 (MQ=60)
 TCAGGGTAATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAA                                                                                                                                                  <  SRR19973066.290723/150‑1 (MQ=60)
   AGGGTAATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAAT                                                                                                                                                <  SRR19973066.1186920/150‑1 (MQ=60)
    GGGTAATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATC                                                                                                                                               <  SRR19973066.3102066/150‑1 (MQ=60)
    GGGTAATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATC                                                                                                                                               <  SRR19973066.3156680/150‑1 (MQ=60)
    GGGTAATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATC                                                                                                                                               <  SRR19973066.2048485/150‑1 (MQ=60)
      GTAATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGACTAAATCGG                                                                                                                                             >  SRR19973066.824875/1‑150 (MQ=60)
        AATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCC                                                                                                                                           >  SRR19973066.1721863/1‑150 (MQ=60)
         ATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCA                                                                                                                                          <  SRR19973066.3382130/150‑1 (MQ=60)
         ATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCA                                                                                                                                          >  SRR19973066.2341509/1‑150 (MQ=60)
         ATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCA                                                                                                                                          <  SRR19973066.1603581/150‑1 (MQ=60)
         ATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCA                                                                                                                                          >  SRR19973066.2131795/1‑150 (MQ=60)
            TTTATGCTGTCCTTCTTTTCATGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGG                                                                                                                                       <  SRR19973066.1904392/150‑1 (MQ=60)
            TTTATGCTGTCCTTCTTTTCAAGTCCCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCACCCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGG                                                                                                                                       <  SRR19973066.3067944/150‑1 (MQ=60)
             TTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGA                                                                                                                                      >  SRR19973066.103310/1‑150 (MQ=60)
               ATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATA                                                                                                                                    <  SRR19973066.442879/150‑1 (MQ=60)
               ATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATA                                                                                                                                    <  SRR19973066.1884704/150‑1 (MQ=60)
                 GCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAAC                                                                                                                                  <  SRR19973066.1502851/150‑1 (MQ=60)
                 GCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAAC                                                                                                                                  <  SRR19973066.1502839/150‑1 (MQ=60)
                    GTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACA                                                                                                                               >  SRR19973066.1151416/1‑150 (MQ=60)
                    GTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACA                                                                                                                               >  SRR19973066.1814543/1‑150 (MQ=60)
                     TCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACAT                                                                                                                              <  SRR19973066.1742468/150‑1 (MQ=60)
                     TCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACAT                                                                                                                              <  SRR19973066.2550411/150‑1 (MQ=60)
                     TCCTTCTTTTCAAGTAGCGGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACAT                                                                                                                              <  SRR19973066.1743296/150‑1 (MQ=60)
                     TCCTTATTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACAT                                                                                                                              <  SRR19973066.1742556/150‑1 (MQ=60)
                       CTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATAC                                                                                                                            >  SRR19973066.2580486/1‑150 (MQ=60)
                       CTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATAC                                                                                                                            >  SRR19973066.401927/1‑150 (MQ=60)
                        TTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATGCA                                                                                                                           >  SRR19973066.739956/1‑150 (MQ=60)
                        TTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACA                                                                                                                           >  SRR19973066.740080/1‑150 (MQ=60)
                         TCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACAC                                                                                                                          >  SRR19973066.3007980/1‑150 (MQ=60)
                         TCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACAC                                                                                                                          <  SRR19973066.3487234/150‑1 (MQ=60)
                         TCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACAC                                                                                                                          <  SRR19973066.3488403/150‑1 (MQ=60)
                           TTTTCAAGTAGCTGACAAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCA                                                                                                                        <  SRR19973066.2234113/150‑1 (MQ=60)
                                 AGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGG                                                                                                                  >  SRR19973066.3478558/1‑150 (MQ=60)
                                 AGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGG                                                                                                                  >  SRR19973066.647010/1‑150 (MQ=60)
                                 AGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGG                                                                                                                  >  SRR19973066.646931/1‑150 (MQ=60)
                                      CTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGTATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCC                                                                                                             <  SRR19973066.2511394/150‑1 (MQ=60)
                                      CTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCC                                                                                                             >  SRR19973066.2078614/1‑150 (MQ=60)
                                      CTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCC                                                                                                             <  SRR19973066.2511199/150‑1 (MQ=60)
                                      CTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCC                                                                                                             >  SRR19973066.617309/1‑150 (MQ=60)
                                      ATGACCAAAAACGACGCTCTAAGAATAATAACGAAATAAACAAATCAGGCAAACAGGATCATCTCACCCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGTAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCC                                                                                                             <  SRR19973066.2511385/150‑1 (MQ=60)
                                       TGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCT                                                                                                            >  SRR19973066.543733/1‑150 (MQ=60)
                                         ACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGG                                                                                                          <  SRR19973066.2069292/150‑1 (MQ=60)
                                           CAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAA                                                                                                        <  SRR19973066.3280359/150‑1 (MQ=60)
                                           CAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAA                                                                                                        <  SRR19973066.1497528/150‑1 (MQ=60)
                                           CAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAA                                                                                                        >  SRR19973066.1452310/1‑150 (MQ=60)
                                            AACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAAC                                                                                                       >  SRR19973066.2305089/1‑150 (MQ=60)
                                            AACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAAC                                                                                                       >  SRR19973066.1456808/1‑150 (MQ=60)
                                             ACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACA                                                                                                      <  SRR19973066.1504191/150‑1 (MQ=60)
                                               AACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGT                                                                                                    >  SRR19973066.1970080/1‑150 (MQ=60)
                                                  GACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACG                                                                                                 >  SRR19973066.578434/1‑150 (MQ=60)
                                                   ACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGA                                                                                                >  SRR19973066.952465/1‑150 (MQ=60)
                                                       GCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCC                                                                                            >  SRR19973066.168548/1‑150 (MQ=60)
                                                           AGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGACCAGTACGAGGCCCTGA                                                                                        >  SRR19973066.2880981/1‑150 (MQ=60)
                                                           AGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGA                                                                                        >  SRR19973066.1688040/1‑150 (MQ=60)
                                                           AGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGA                                                                                        >  SRR19973066.1688039/1‑150 (MQ=60)
                                                            GACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAG                                                                                       <  SRR19973066.2667479/150‑1 (MQ=60)
                                                               TAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGA                                                                                    >  SRR19973066.3261497/1‑150 (MQ=60)
                                                               TAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGA                                                                                    >  SRR19973066.3261491/1‑150 (MQ=60)
                                                                AATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAG                                                                                   >  SRR19973066.3172394/1‑150 (MQ=60)
                                                                  TAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCA                                                                                 <  SRR19973066.1631928/150‑1 (MQ=60)
                                                                   AACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAG                                                                                >  SRR19973066.3529706/1‑150 (MQ=60)
                                                                   AACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAG                                                                                >  SRR19973066.3528630/1‑150 (MQ=60)
                                                                   AACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAG                                                                                >  SRR19973066.3390013/1‑150 (MQ=60)
                                                                   AACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAG                                                                                >  SRR19973066.1609353/1‑150 (MQ=60)
                                                                           AAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCA                                                                        >  SRR19973066.2954057/1‑150 (MQ=60)
                                                                           AAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCA                                                                        >  SRR19973066.2954193/1‑150 (MQ=60)
                                                                            AACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCAC                                                                       >  SRR19973066.2857999/1‑150 (MQ=60)
                                                                              CAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCA                                                                     <  SRR19973066.198384/150‑1 (MQ=60)
                                                                              CAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCA                                                                     <  SRR19973066.2013154/150‑1 (MQ=60)
                                                                              CAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCA                                                                     <  SRR19973066.198367/150‑1 (MQ=60)
                                                                               AAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCAT                                                                    >  SRR19973066.271300/1‑150 (MQ=60)
                                                                                   CCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCG                                                                <  SRR19973066.823960/150‑1 (MQ=60)
                                                                                          ACAGGATCATCTCACCCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACG                                                         <  SRR19973066.3134676/150‑1 (MQ=60)
                                                                                          ACACGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACG                                                         <  SRR19973066.3419888/150‑1 (MQ=60)
                                                                                             GGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCT                                                      <  SRR19973066.3559657/150‑1 (MQ=60)
                                                                                             GGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCT                                                      <  SRR19973066.705746/150‑1 (MQ=60)
                                                                                              GATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATTCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTG                                                     >  SRR19973066.3170115/1‑150 (MQ=60)
                                                                                                TCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCG                                                   >  SRR19973066.2080164/1‑150 (MQ=60)
                                                                                                 CATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGG                                                  >  SRR19973066.2907193/1‑150 (MQ=60)
                                                                                                  ATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGC                                                 >  SRR19973066.2369179/1‑150 (MQ=60)
                                                                                                   TCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCA                                                >  SRR19973066.3194566/1‑150 (MQ=60)
                                                                                                   TCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCA                                                >  SRR19973066.1973790/1‑150 (MQ=60)
                                                                                                     TCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGA                                              <  SRR19973066.2883391/150‑1 (MQ=60)
                                                                                                       AACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAAT                                            <  SRR19973066.3149814/150‑1 (MQ=60)
                                                                                                             CGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAG                                      <  SRR19973066.2111082/150‑1 (MQ=60)
                                                                                                                ACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTT                                   <  SRR19973066.1090583/150‑1 (MQ=60)
                                                                                                                ACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTT                                   <  SRR19973066.662969/150‑1 (MQ=60)
                                                                                                                  CATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCA                                 >  SRR19973066.1181812/1‑150 (MQ=60)
                                                                                                                    TCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATC                               <  SRR19973066.2362351/150‑1 (MQ=60)
                                                                                                                    TCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATC                               <  SRR19973066.2403693/150‑1 (MQ=60)
                                                                                                                    TCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATC                               >  SRR19973066.1140405/1‑150 (MQ=60)
                                                                                                                    TCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATC                               >  SRR19973066.1140404/1‑150 (MQ=60)
                                                                                                                       CGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGAC                            <  SRR19973066.2639867/150‑1 (MQ=60)
                                                                                                                           CACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCAGGAACAGTACGATGCCCTGAGTGAGCAGCACCGGCACCAACGCGCGGAACCCCTGCGGCAGAATAATCAGTTTCAACGACTGCC                        >  SRR19973066.3290147/1‑150 (MQ=60)
                                                                                                                             CCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAG                      >  SRR19973066.461176/1‑150 (MQ=60)
                                                                                                                               AATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTG                    <  SRR19973066.2804837/150‑1 (MQ=60)
                                                                                                                                          CGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCA         <  SRR19973066.2796895/150‑1 (MQ=60)
                                                                                                                                               CGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCC    >  SRR19973066.3187475/1‑150 (MQ=60)
                                                                                                                                                GGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCA   <  SRR19973066.166762/150‑1 (MQ=60)
                                                                                                                                                GGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCA   <  SRR19973066.93250/150‑1 (MQ=60)
                                                                                                                                                 GAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAG  >  SRR19973066.3535544/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                  
TTCAGGGTAATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAG  >  CP009273/680698‑680992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑