Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 680,846 G→T 100% F180L (TTC→TTA)  gltK ← glutamate, aspartate ABC transporter permease subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273680,8460GT99.5% 701.6 / ‑4.3 214F180L (TTC→TTA) gltKglutamate, aspartate ABC transporter permease subunit
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (138/75);  total (139/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 218 total aligned reads displayed.

AGGGTAATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCC  >  CP009273/680701‑680990
                                                                                                                                                 |                                                                                                                                                
     aaTCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCgg                                                                                                                                           <  1:2790018/148‑1 (MQ=255)
      aTCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGc                                                                                                                                          >  1:1005235/1‑148 (MQ=255)
      aTCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGc                                                                                                                                          >  1:1247274/1‑148 (MQ=255)
      aTCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGc                                                                                                                                          <  2:188444/148‑1 (MQ=255)
       tCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGcc                                                                                                                                         >  1:596238/1‑148 (MQ=255)
              gCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATa                                                                                                                                  >  2:121637/1‑148 (MQ=255)
              gCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATa                                                                                                                                  >  2:1122329/1‑148 (MQ=255)
               cTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATaa                                                                                                                                 >  2:785050/1‑148 (MQ=255)
               cTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATaa                                                                                                                                 <  2:589672/148‑1 (MQ=255)
                tGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAac                                                                                                                                >  1:1322335/1‑148 (MQ=255)
                 gTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAaca                                                                                                                               >  1:302024/1‑148 (MQ=255)
                  tCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAacac                                                                                                                              >  2:1384855/1‑148 (MQ=255)
                  tCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAacac                                                                                                                              <  1:2925177/148‑1 (MQ=255)
                  tCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAacac                                                                                                                              <  2:1338234/148‑1 (MQ=255)
                     ttcttTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATa                                                                                                                           >  2:2841470/1‑148 (MQ=255)
                       cttTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATaca                                                                                                                         <  2:2008435/148‑1 (MQ=255)
                       cttTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATaca                                                                                                                         <  2:233543/148‑1 (MQ=255)
                         tttCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACAcc                                                                                                                       >  2:1955979/1‑148 (MQ=255)
                            cAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGt                                                                                                                    >  2:1102014/1‑148 (MQ=255)
                              aGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGa                                                                                                                  <  2:1039190/148‑1 (MQ=255)
                               gTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAg                                                                                                                 >  2:2552090/1‑148 (MQ=255)
                                  gCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTa                                                                                                              >  2:85164/1‑148 (MQ=255)
                                      aCCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCt                                                                                                          >  2:1094907/1‑148 (MQ=255)
                                      aCCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCt                                                                                                          >  2:1094937/1‑148 (MQ=255)
                                       ccAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTg                                                                                                         <  1:440870/148‑1 (MQ=255)
                                       ccAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTg                                                                                                         <  1:440834/148‑1 (MQ=255)
                                        caacaaCGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTgg                                                                                                        >  2:1689386/1‑148 (MQ=255)
                                         aacaacGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGa                                                                                                       >  2:1749620/1‑148 (MQ=255)
                                            aacGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACa                                                                                                    >  1:3499458/1‑148 (MQ=255)
                                            aacGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACa                                                                                                    <  2:2354342/148‑1 (MQ=255)
                                            aacGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACa                                                                                                    >  2:1671980/1‑148 (MQ=255)
                                             acgacgCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTCTCCTGGAACAg                                                                                                   >  2:1916035/1‑148 (MQ=255)
                                              cgacgCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGt                                                                                                  >  1:2209411/1‑148 (MQ=255)
                                                acgCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGGTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTAc                                                                                                >  2:1815426/1‑148 (MQ=255)
                                                 cgcgCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACg                                                                                               <  2:3226654/148‑1 (MQ=255)
                                                    gcTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATg                                                                                            <  2:2306941/148‑1 (MQ=255)
                                                     cTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGc                                                                                           >  1:1358538/1‑148 (MQ=255)
                                                      tAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGcc                                                                                          <  1:2144812/148‑1 (MQ=255)
                                                         gACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCtg                                                                                       >  1:1852529/1‑148 (MQ=255)
                                                            taataaCGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCtgagt                                                                                    >  1:1891260/1‑148 (MQ=255)
                                                            taataaCGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCtgagt                                                                                    >  1:778219/1‑148 (MQ=255)
                                                             aataaCGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCtgagtg                                                                                   >  2:906936/1‑148 (MQ=255)
                                                               taaCGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCtgagtgag                                                                                 <  1:1580734/148‑1 (MQ=255)
                                                                  cGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGagcag                                                                              >  2:2142092/1‑148 (MQ=255)
                                                                  cGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGagcag                                                                              >  2:2905384/1‑148 (MQ=255)
                                                                   gAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGagcagc                                                                             <  1:1975744/148‑1 (MQ=255)
                                                                    aaaTAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGagcagca                                                                            >  1:2577652/1‑148 (MQ=255)
                                                                    aaaTAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGagcagca                                                                            >  1:1451435/1‑148 (MQ=255)
                                                                       tAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCg                                                                         >  2:3384769/1‑148 (MQ=255)
                                                                          aCAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCa                                                                      >  1:1165027/1‑148 (MQ=255)
                                                                          aCAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCa                                                                      >  1:3151683/1‑148 (MQ=255)
                                                                            aaaaCCGGCAAACAGGATAATATCAAACTGCGTACCATCACGCTCACCAATGGTTGAGTCGGTACGGAATAAATAGGCCAGGGATAACACATACACCAGTGAGGTATACTGCAACAGTACGATGCCCTGAGTGATCAGAAGCGGCAcc                                                                    >  2:1612416/1‑148 (MQ=255)
                                                                             aaTCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGGGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCa                                                                   >  1:1848062/1‑148 (MQ=255)
                                                                             aaTCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCa                                                                   >  1:2112753/1‑148 (MQ=255)
                                                                             aaTCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCa                                                                   >  1:343593/1‑148 (MQ=255)
                                                                             aaTCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCa                                                                   <  2:494750/148‑1 (MQ=255)
                                                                              aTCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCAt                                                                  >  2:340037/1‑148 (MQ=255)
                                                                               tCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATc                                                                 >  1:100220/1‑148 (MQ=255)
                                                                                     aaaCAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCgg                                                           >  1:3174201/1‑148 (MQ=255)
                                                                                     aaaCAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCgg                                                           >  1:3175350/1‑148 (MQ=255)
                                                                                     aaaCAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCgg                                                           >  2:866230/1‑148 (MQ=255)
                                                                                     aaaCAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCgg                                                           >  2:866354/1‑148 (MQ=255)
                                                                                        cAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAAc                                                        >  2:1047578/1‑148 (MQ=255)
                                                                                        cAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAAc                                                        >  1:1910592/1‑148 (MQ=255)
                                                                                        cAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAAc                                                        >  1:1502451/1‑148 (MQ=255)
                                                                                        cAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAAc                                                        >  1:763453/1‑148 (MQ=255)
                                                                                         aGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACg                                                       <  2:1005235/148‑1 (MQ=255)
                                                                                         aGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACg                                                       <  2:1247274/148‑1 (MQ=255)
                                                                                          ggATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGc                                                      >  2:2591439/1‑148 (MQ=255)
                                                                                                 ctcAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGc                                               >  2:1652727/1‑148 (MQ=255)
                                                                                                   cAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAg                                             >  1:562846/1‑148 (MQ=255)
                                                                                                     aCCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGaa                                           >  1:461702/1‑148 (MQ=255)
                                                                                                       cTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGaata                                         <  1:2367686/148‑1 (MQ=255)
                                                                                                       cTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGaata                                         >  1:3054986/1‑148 (MQ=255)
                                                                                                        tGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGaataa                                        <  1:3017468/148‑1 (MQ=255)
                                                                                                         gCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGaataat                                       >  1:3301272/1‑148 (MQ=255)
                                                                                                           gTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCa                                     <  1:1822237/148‑1 (MQ=255)
                                                                                                            tACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAg                                    >  1:3493322/1‑148 (MQ=255)
                                                                                                             aCCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGt                                   >  2:2293035/1‑148 (MQ=255)
                                                                                                             aCCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGt                                   <  1:2980859/148‑1 (MQ=255)
                                                                                                               cATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGttt                                 >  2:1165571/1‑148 (MQ=255)
                                                                                                                aTCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTc                                <  1:1324417/148‑1 (MQ=255)
                                                                                                                aTCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTc                                <  2:556319/148‑1 (MQ=255)
                                                                                                                 tCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCa                               >  2:3203283/1‑148 (MQ=255)
                                                                                                                 tCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCa                               <  1:3322793/148‑1 (MQ=255)
                                                                                                                        cACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTg                        >  2:2124222/1‑148 (MQ=255)
                                                                                                                         aCCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGc                       >  2:249981/1‑148 (MQ=255)
                                                                                                                         aCCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGc                       >  1:2256902/1‑148 (MQ=255)
                                                                                                                            aaTGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAg                    >  2:3418713/1‑148 (MQ=255)
                                                                                                                            aaTGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAg                    <  1:1401150/148‑1 (MQ=255)
                                                                                                                              tGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTg                  >  2:373064/1‑148 (MQ=255)
                                                                                                                                 ttGAGGCGGTACGTAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCNGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTCCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGt               <  1:1780918/148‑1 (MQ=255)
                                                                                                                                 ttGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGt               >  1:1385259/1‑148 (MQ=255)
                                                                                                                                    aGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCAt            >  2:759705/1‑148 (MQ=255)
                                                                                                                                       cGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGATGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCCGTTTCATCGACTGCCAGTGAGTCATTcc         <  2:910339/148‑1 (MQ=255)
                                                                                                                                       cGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTcc         <  1:952292/148‑1 (MQ=255)
                                                                                                                                       cGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTcc         <  1:1017530/148‑1 (MQ=255)
                                                                                                                                          tACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCaa      >  1:1088419/1‑148 (MQ=255)
                                                                                                                                           aCGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAAc     >  2:1539643/1‑148 (MQ=255)
                                                                                                                                             ggAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGc   <  1:1955979/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AGGGTAATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCC  >  CP009273/680701‑680990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGGGTAATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAG  >  CP009273/680701‑680992
                                                                                                                                                 |                                                                                                                                                  
AGGGTAATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAAT                                                                                                                                                <  SRR19973065.1446217/150‑1 (MQ=60)
    TAATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGC                                                                                                                                            >  SRR19973065.1011910/1‑150 (MQ=60)
    TAATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGC                                                                                                                                            >  SRR19973065.1255489/1‑150 (MQ=60)
     AATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCC                                                                                                                                           >  SRR19973065.599936/1‑150 (MQ=60)
     AATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCC                                                                                                                                           <  SRR19973065.2807311/150‑1 (MQ=60)
             TGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAA                                                                                                                                   <  SRR19973065.2145467/150‑1 (MQ=60)
              GCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAAC                                                                                                                                  >  SRR19973065.1331597/1‑150 (MQ=60)
              GCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAAC                                                                                                                                  >  SRR19973065.1331078/1‑150 (MQ=60)
               CTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACA                                                                                                                                 <  SRR19973065.782863/150‑1 (MQ=60)
               CTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACA                                                                                                                                 >  SRR19973065.337463/1‑150 (MQ=60)
               CTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACA                                                                                                                                 >  SRR19973065.303997/1‑150 (MQ=60)
                  TCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACAT                                                                                                                              <  SRR19973065.2942948/150‑1 (MQ=60)
                    CTTATTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATAC                                                                                                                            <  SRR19973065.969618/150‑1 (MQ=60)
                                TAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATACGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACAAATACACCAGTGAGGTA                                                                                                                >  SRR19973065.1044880/1‑150 (MQ=60)
                                   CTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCC                                                                                                             >  SRR19973065.1862286/1‑150 (MQ=60)
                                   CTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCC                                                                                                             >  SRR19973065.1861888/1‑150 (MQ=60)
                                    TGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCT                                                                                                            <  SRR19973065.1016525/150‑1 (MQ=60)
                                       CCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGA                                                                                                         <  SRR19973065.443924/150‑1 (MQ=60)
                                       CCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGA                                                                                                         <  SRR19973065.443892/150‑1 (MQ=60)
                                       CCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGA                                                                                                         <  SRR19973065.443888/150‑1 (MQ=60)
                                          ACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACA                                                                                                      >  SRR19973065.3520716/1‑150 (MQ=60)
                                          ACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACA                                                                                                      >  SRR19973065.3520690/1‑150 (MQ=60)
                                            AACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGT                                                                                                    >  SRR19973065.2224035/1‑150 (MQ=60)
                                            AACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGT                                                                                                    >  SRR19973065.2223537/1‑150 (MQ=60)
                                                   CGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGC                                                                                             >  SRR19973065.1367525/1‑150 (MQ=60)
                                                   CGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGC                                                                                             >  SRR19973065.1367537/1‑150 (MQ=60)
                                                     CTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCC                                                                                           >  SRR19973065.560148/1‑150 (MQ=60)
                                                      TAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCT                                                                                          >  SRR19973065.2660895/1‑150 (MQ=60)
                                                      TAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCT                                                                                          <  SRR19973065.2158605/150‑1 (MQ=60)
                                                      TAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCT                                                                                          <  SRR19973065.299997/150‑1 (MQ=60)
                                                      AAAGACTAAAAACGAAATAAACAAATCCGGCAAACAGTATCATCTCAACCTGCTTACCATCACTCTCACCAATGGTTGAGGCGTTACGGATTAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCT                                                                                          <  SRR19973065.2158099/150‑1 (MQ=60)
                                                       AAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTG                                                                                         >  SRR19973065.1864732/1‑150 (MQ=60)
                                                       AAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTG                                                                                         >  SRR19973065.1864736/1‑150 (MQ=60)
                                                          ACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGT                                                                                      >  SRR19973065.1832354/1‑150 (MQ=60)
                                                          ACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGT                                                                                      >  SRR19973065.1903800/1‑150 (MQ=60)
                                                          ACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGT                                                                                      >  SRR19973065.1903694/1‑150 (MQ=60)
                                                          ACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGT                                                                                      >  SRR19973065.783391/1‑150 (MQ=60)
                                                             AATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAG                                                                                   <  SRR19973065.3197461/150‑1 (MQ=60)
                                                               TAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCA                                                                                 <  SRR19973065.1591273/150‑1 (MQ=60)
                                                                  CGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCA                                                                              >  SRR19973065.2593730/1‑150 (MQ=60)
                                                                  CGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCA                                                                              >  SRR19973065.1460798/1‑150 (MQ=60)
                                                                  CGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATAAACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCA                                                                              >  SRR19973065.1973329/1‑150 (MQ=60)
                                                                   GAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAG                                                                             <  SRR19973065.1988603/150‑1 (MQ=60)
                                                                     AATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCG                                                                           >  SRR19973065.3157244/1‑150 (MQ=60)
                                                                     AATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCG                                                                           >  SRR19973065.3157226/1‑150 (MQ=60)
                                                                     AATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCG                                                                           >  SRR19973065.2079518/1‑150 (MQ=60)
                                                                     AATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCG                                                                           >  SRR19973065.279032/1‑150 (MQ=60)
                                                                        AAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCA                                                                        >  SRR19973065.1172470/1‑150 (MQ=60)
                                                                        AAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCA                                                                        >  SRR19973065.3170807/1‑150 (MQ=60)
                                                                        AAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCA                                                                        >  SRR19973065.3171239/1‑150 (MQ=60)
                                                                           CAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGGGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCA                                                                     >  SRR19973065.1860240/1‑150 (MQ=60)
                                                                           CAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCA                                                                     >  SRR19973065.1860213/1‑150 (MQ=60)
                                                                           CAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCA                                                                     >  SRR19973065.2126352/1‑150 (MQ=60)
                                                                           CAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCA                                                                     >  SRR19973065.345993/1‑150 (MQ=60)
                                                                             AATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATC                                                                   >  SRR19973065.101139/1‑150 (MQ=60)
                                                                             AATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATC                                                                   >  SRR19973065.101198/1‑150 (MQ=60)
                                                                                   GCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGG                                                             >  SRR19973065.3193397/1‑150 (MQ=60)
                                                                                   GCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGG                                                             >  SRR19973065.3194544/1‑150 (MQ=60)
                                                                                   GCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGG                                                             >  SRR19973065.3194549/1‑150 (MQ=60)
                                                                                   GCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGG                                                             >  SRR19973065.1613990/1‑150 (MQ=60)
                                                                                   GCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGG                                                             <  SRR19973065.544605/150‑1 (MQ=60)
                                                                                      AACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAAC                                                          >  SRR19973065.768533/1‑150 (MQ=60)
                                                                                      AACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAAC                                                          >  SRR19973065.1923139/1‑150 (MQ=60)
                                                                                      AACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAAC                                                          >  SRR19973065.1512173/1‑150 (MQ=60)
                                                                                      AACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAAC                                                          >  SRR19973065.1512167/1‑150 (MQ=60)
                                                                                         AGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCC                                                       >  SRR19973065.2215428/1‑150 (MQ=60)
                                                                                         AGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCC                                                       >  SRR19973065.1496870/1‑150 (MQ=60)
                                                                                         AGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCC                                                       >  SRR19973065.2409574/1‑150 (MQ=60)
                                                                                             TCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCG                                                   <  SRR19973065.3405465/150‑1 (MQ=60)
                                                                                               ATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGC                                                 >  SRR19973065.3475154/1‑150 (MQ=60)
                                                                                               ATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGC                                                 >  SRR19973065.12146/1‑150 (MQ=60)
                                                                                                 CTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAG                                               >  SRR19973065.566447/1‑150 (MQ=60)
                                                                                                 CTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAG                                               >  SRR19973065.566426/1‑150 (MQ=60)
                                                                                                   CAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAA                                             >  SRR19973065.464890/1‑150 (MQ=60)
                                                                                                   CAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAA                                             >  SRR19973065.464808/1‑150 (MQ=60)
                                                                                                    AACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAAT                                            >  SRR19973065.2371117/1‑150 (MQ=60)
                                                                                                     ACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATA                                           >  SRR19973065.3073663/1‑150 (MQ=60)
                                                                                                     ACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATA                                           >  SRR19973065.3073478/1‑150 (MQ=60)
                                                                                                       CTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAAT                                         >  SRR19973065.1398415/1‑150 (MQ=60)
                                                                                                       CTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAAT                                         <  SRR19973065.1393964/150‑1 (MQ=60)
                                                                                                       CTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAAT                                         >  SRR19973065.3321376/1‑150 (MQ=60)
                                                                                                       CTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAAT                                         >  SRR19973065.3322119/1‑150 (MQ=60)
                                                                                                       CTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAAT                                         <  SRR19973065.2382441/150‑1 (MQ=60)
                                                                                                       CTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAAT                                         <  SRR19973065.442672/150‑1 (MQ=60)
                                                                                                        TGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATC                                        <  SRR19973065.3035950/150‑1 (MQ=60)
                                                                                                        TGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATC                                        <  SRR19973065.3035196/150‑1 (MQ=60)
                                                                                                        TGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATC                                        <  SRR19973065.3035967/150‑1 (MQ=60)
                                                                                                          CGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAG                                      >  SRR19973065.3514559/1‑150 (MQ=60)
                                                                                                           GTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGT                                     <  SRR19973065.1834235/150‑1 (MQ=60)
                                                                                                             ACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTT                                   <  SRR19973065.2999096/150‑1 (MQ=60)
                                                                                                                ATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCAT                                <  SRR19973065.1333174/150‑1 (MQ=60)
                                                                                                                 TCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATC                               <  SRR19973065.3343065/150‑1 (MQ=60)
                                                                                                                       TCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGC                         >  SRR19973065.805711/1‑150 (MQ=60)
                                                                                                                       TCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGC                         >  SRR19973065.2271187/1‑150 (MQ=60)
                                                                                                                            AATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTG                    >  SRR19973065.1272211/1‑150 (MQ=60)
                                                                                                                            AATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTG                    <  SRR19973065.1410337/150‑1 (MQ=60)
                                                                                                                            AATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTG                    >  SRR19973065.1272367/1‑150 (MQ=60)
                                                                                                                               GGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATCCACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGT                 <  SRR19973065.696364/150‑1 (MQ=60)
                                                                                                                               GGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGT                 >  SRR19973065.1394368/1‑150 (MQ=60)
                                                                                                                                 TTGAGGCGGTACGTAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCNGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTCCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCA               <  SRR19973065.1792546/150‑1 (MQ=60)
                                                                                                                                       CGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCA         <  SRR19973065.1024273/150‑1 (MQ=60)
                                                                                                                                       CGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCA         <  SRR19973065.958587/150‑1 (MQ=60)
                                                                                                                                        GGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAA        >  SRR19973065.1095592/1‑150 (MQ=60)
                                                                                                                                        GGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAA        >  SRR19973065.1095772/1‑150 (MQ=60)
                                                                                                                                            CGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCC    >  SRR19973065.1465784/1‑150 (MQ=60)
                                                                                                                                             GGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCA   <  SRR19973065.1968761/150‑1 (MQ=60)
                                                                                                                                             GGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCA   <  SRR19973065.1968717/150‑1 (MQ=60)
                                                                                                                                              GAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAG  <  SRR19973065.2858959/150‑1 (MQ=60)
                                                                                                                                              GAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAG  >  SRR19973065.644376/1‑150 (MQ=60)
                                                                                                                                              GAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAG  >  SRR19973065.644380/1‑150 (MQ=60)
                                                                                                                                              GAAAAAATCGGCCAGGGATAACACATACACCAGAGAGTTATCCTGGAACAGTACTATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCAAG  >  SRR19973065.1163129/1‑150 (MQ=60)
                                                                                                                                                 |                                                                                                                                                  
AGGGTAATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAG  >  CP009273/680701‑680992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑