Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 828,374 G→T 100% V11V (GTC→GTA)  ybiA ← DUF1768 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273828,3740GT99.4% 505.7 / ‑4.1 153V11V (GTC→GTA) ybiADUF1768 family protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (61/91);  total (62/91)
Fisher's exact test for biased strand distribution p-value = 4.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 153 total aligned reads displayed.

TTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGG  >  CP009273/828230‑828518
                                                                                                                                                |                                                                                                                                                
ttCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCTGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACg                                                                                                                                               <  1:1156642/148‑1 (MQ=255)
 tcatcaAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACgt                                                                                                                                              >  1:584498/1‑148 (MQ=255)
  catcaAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACgtg                                                                                                                                             >  2:662017/1‑148 (MQ=255)
  catcaAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACgtg                                                                                                                                             >  2:2325324/1‑148 (MQ=255)
  catcaAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACgtg                                                                                                                                             <  2:3245796/148‑1 (MQ=255)
   atcaAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCGTTACgtgt                                                                                                                                            <  2:1252585/148‑1 (MQ=255)
     caAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTg                                                                                                                                          >  1:1070294/1‑148 (MQ=255)
      aaGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTgg                                                                                                                                         <  1:970293/148‑1 (MQ=255)
      aaGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTgg                                                                                                                                         <  1:3067038/148‑1 (MQ=255)
      aaGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTgg                                                                                                                                         <  1:2943585/148‑1 (MQ=255)
       aGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCTGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGa                                                                                                                                        >  2:1225894/1‑148 (MQ=255)
       aGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGa                                                                                                                                        >  2:1503500/1‑148 (MQ=255)
       aGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGa                                                                                                                                        >  2:2506448/1‑148 (MQ=255)
          aaTTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATcc                                                                                                                                     <  1:996180/148‑1 (MQ=255)
          aaTTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATcc                                                                                                                                     <  1:3224193/148‑1 (MQ=255)
            tttttGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCtt                                                                                                                                   >  2:2789979/1‑148 (MQ=255)
            tttttGGGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCtt                                                                                                                                   <  2:655472/148‑1 (MQ=255)
                  tgCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCt                                                                                                                             <  2:1924457/148‑1 (MQ=255)
                     cTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGa                                                                                                                          <  2:1041062/148‑1 (MQ=255)
                      tGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGaa                                                                                                                         <  1:547211/148‑1 (MQ=255)
                       gAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAAc                                                                                                                        >  1:1309615/1‑148 (MQ=255)
                            tAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCa                                                                                                                   >  1:2307276/1‑148 (MQ=255)
                            tAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCa                                                                                                                   <  1:2506391/148‑1 (MQ=255)
                            tAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCa                                                                                                                   <  1:2262293/148‑1 (MQ=255)
                             aGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCAt                                                                                                                  <  2:2955306/148‑1 (MQ=255)
                              gtgtTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCAtt                                                                                                                 >  1:1190752/1‑148 (MQ=255)
                                 ttCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTaaa                                                                                                              <  2:542732/148‑1 (MQ=255)
                                  tCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAAc                                                                                                             <  2:2246681/148‑1 (MQ=255)
                                     gAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCTGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGc                                                                                                          <  1:1934161/148‑1 (MQ=255)
                                      aGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCa                                                                                                         <  2:2521481/148‑1 (MQ=255)
                                      aGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCa                                                                                                         <  2:2340167/148‑1 (MQ=255)
                                        gTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCAtt                                                                                                       <  2:3231488/148‑1 (MQ=255)
                                          aGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTAt                                                                                                     <  2:1086905/148‑1 (MQ=255)
                                           ggCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATg                                                                                                    <  2:1310795/148‑1 (MQ=255)
                                               aGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTtgg                                                                                                >  2:1736843/1‑148 (MQ=255)
                                                  ttttCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTtggtgg                                                                                             >  2:2555702/1‑148 (MQ=255)
                                                   tttCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTggtggt                                                                                            <  1:2224620/148‑1 (MQ=255)
                                                    ttCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGtt                                                                                           <  1:3173045/148‑1 (MQ=255)
                                                     tCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTa                                                                                          <  1:2497236/148‑1 (MQ=255)
                                                      cccGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTAt                                                                                         <  1:3293260/148‑1 (MQ=255)
                                                      cccGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGGGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTAt                                                                                         <  1:3293316/148‑1 (MQ=255)
                                                        cGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTg                                                                                       <  2:382558/148‑1 (MQ=255)
                                                        cGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTg                                                                                       <  2:2624215/148‑1 (MQ=255)
                                                          tCAACCTTGATGGGCCAGGCGGTAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTgag                                                                                     >  2:3012618/1‑146 (MQ=255)
                                                          tCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTCTGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGACCGGGCATTAAACCGCATTATGTTGGTGGTTATTGCg                                                                                     >  2:560653/1‑148 (MQ=255)
                                                               cTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCg                                                                                <  1:2592229/148‑1 (MQ=255)
                                                               cTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCg                                                                                <  2:1541695/148‑1 (MQ=255)
                                                                ttGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGc                                                                               <  1:195549/148‑1 (MQ=255)
                                                                        cctggcggcAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCcaga                                                                       <  1:1225894/145‑1 (MQ=255)
                                                                          aGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCcagaaa                                                                     <  2:2758337/148‑1 (MQ=255)
                                                                           ggcggcAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCcagaaac                                                                    <  2:888115/148‑1 (MQ=255)
                                                                           ggcggcAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCcagaaac                                                                    >  1:3079332/1‑148 (MQ=255)
                                                                             cggcAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCcagaaacag                                                                  <  1:2156918/148‑1 (MQ=255)
                                                                              ggcAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCcagaaacaga                                                                 <  2:1822209/148‑1 (MQ=255)
                                                                                cAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCcagaaacagaaa                                                               >  2:908898/1‑148 (MQ=255)
                                                                                  aaGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGaaaaa                                                             >  2:709042/1‑148 (MQ=255)
                                                                                   aGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAAc                                                            >  2:655216/1‑148 (MQ=255)
                                                                                    gTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAAcc                                                           >  1:2933811/1‑148 (MQ=255)
                                                                                     ttAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCa                                                          <  1:1946864/148‑1 (MQ=255)
                                                                                       aGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCAtt                                                        <  1:178684/148‑1 (MQ=255)
                                                                                           aaaTCACCGTAGTCATCGCTGGTGCTGTAGAACTTTATGATGGTTACTTGCATTACGTGTATGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGAGGTGATAGCGAGCCGCTTTCAAGAAACAGAAAAACCATTAcaa                                                    >  1:2923172/1‑146 (MQ=255)
                                                                                           aaaTCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTAccc                                                    <  1:2661761/148‑1 (MQ=255)
                                                                                           aaaTCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTAccc                                                    <  2:196211/148‑1 (MQ=255)
                                                                                             aTCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCt                                                  >  1:1621035/1‑148 (MQ=255)
                                                                                                  cGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGaaaa                                             >  2:2051233/1‑148 (MQ=255)
                                                                                                  cGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGaaaa                                             <  1:1360317/148‑1 (MQ=255)
                                                                                                      gTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGa                                         >  2:1824859/1‑148 (MQ=255)
                                                                                                       tCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGaa                                        >  2:296436/1‑148 (MQ=255)
                                                                                                        cATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGaaa                                       >  2:2052079/1‑148 (MQ=255)
                                                                                                        cATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGaaa                                       >  1:2208061/1‑148 (MQ=255)
                                                                                                         aTCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGaaaa                                      <  1:1898689/148‑1 (MQ=255)
                                                                                                          tCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGaaaaa                                     >  2:3084665/1‑148 (MQ=255)
                                                                                                          tCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGaaaaa                                     >  2:1567409/1‑148 (MQ=255)
                                                                                                           cGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAAt                                    >  1:1389720/1‑148 (MQ=255)
                                                                                                           cGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAAt                                    >  1:7522/1‑148 (MQ=255)
                                                                                                              tGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGcc                                 <  2:2200015/148‑1 (MQ=255)
                                                                                                               ggTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCca                                <  1:338197/148‑1 (MQ=255)
                                                                                                               ggTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCca                                <  1:338488/148‑1 (MQ=255)
                                                                                                                gTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCcac                               <  1:1993886/148‑1 (MQ=255)
                                                                                                                gTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCcac                               <  1:1567033/148‑1 (MQ=255)
                                                                                                                gTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCcac                               >  1:1778906/1‑148 (MQ=255)
                                                                                                                gTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCcac                               >  1:1778904/1‑148 (MQ=255)
                                                                                                                  gCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTCGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACaa                             >  1:2734101/1‑148 (MQ=255)
                                                                                                                   cTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAat                            <  2:759056/148‑1 (MQ=255)
                                                                                                                          aaTTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCt                     <  2:2874642/148‑1 (MQ=255)
                                                                                                                          aaTTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCt                     <  1:1615545/148‑1 (MQ=255)
                                                                                                                           aTTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTg                    <  2:761540/148‑1 (MQ=255)
                                                                                                                            tttATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGt                   <  1:747698/148‑1 (MQ=255)
                                                                                                                            tttATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGt                   <  2:795700/148‑1 (MQ=255)
                                                                                                                                tgatgGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTtat               <  1:1088068/148‑1 (MQ=255)
                                                                                                                                 gatgGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTtata              >  2:1325300/1‑148 (MQ=255)
                                                                                                                                  atgGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATAc             <  2:584498/148‑1 (MQ=255)
                                                                                                                                    ggTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAg           >  2:2767982/1‑148 (MQ=255)
                                                                                                                                    ggTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAg           >  2:1565431/1‑148 (MQ=255)
                                                                                                                                     gtgtCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGt          >  2:1988518/1‑148 (MQ=255)
                                                                                                                                     gtgtCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGt          <  2:1723274/148‑1 (MQ=255)
                                                                                                                                      tgtCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTa         <  1:1524840/148‑1 (MQ=255)
                                                                                                                                      tgtCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTa         >  2:1045507/1‑148 (MQ=255)
                                                                                                                                           tGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCa    >  2:541026/1‑148 (MQ=255)
                                                                                                                                           tGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCa    <  1:2744230/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGG  >  CP009273/828230‑828518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGG  >  CP009273/828225‑828518
                                                                                                                                                     |                                                                                                                                                
TATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATT                                                                                                                                                  <  SRR19973070.991021/150‑1 (MQ=60)
TATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATT                                                                                                                                                  >  SRR19973070.1048412/1‑150 (MQ=60)
 ATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCTGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTA                                                                                                                                                 >  SRR19973070.2711919/1‑150 (MQ=60)
 ATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTA                                                                                                                                                 >  SRR19973070.2399714/1‑150 (MQ=60)
 ATTTTACATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTA                                                                                                                                                 <  SRR19973070.3184051/150‑1 (MQ=60)
    TTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGT                                                                                                                                              >  SRR19973070.588528/1‑150 (MQ=60)
    TTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGT                                                                                                                                              >  SRR19973070.2261657/1‑150 (MQ=60)
     TTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCTGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTG                                                                                                                                             <  SRR19973070.1164721/150‑1 (MQ=60)
        ATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTG                                                                                                                                          >  SRR19973070.1077780/1‑150 (MQ=60)
           AAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGAT                                                                                                                                       <  SRR19973070.977221/150‑1 (MQ=60)
           AAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGAT                                                                                                                                       <  SRR19973070.3086704/150‑1 (MQ=60)
           AAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGAT                                                                                                                                       <  SRR19973070.2962549/150‑1 (MQ=60)
               AATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTT                                                                                                                                   <  SRR19973070.1003320/150‑1 (MQ=60)
               AATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTT                                                                                                                                   <  SRR19973070.3245027/150‑1 (MQ=60)
               AATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTT                                                                                                                                   <  SRR19973070.3074124/150‑1 (MQ=60)
               AATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTT                                                                                                                                   <  SRR19973070.1003284/150‑1 (MQ=60)
                          CTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAAC                                                                                                                        >  SRR19973070.1318703/1‑150 (MQ=60)
                          CTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAAC                                                                                                                        >  SRR19973070.1735207/1‑150 (MQ=60)
                           TGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACG                                                                                                                       <  SRR19973070.550862/150‑1 (MQ=60)
                               AATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCA                                                                                                                   >  SRR19973070.2322615/1‑150 (MQ=60)
                                 TAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATT                                                                                                                 <  SRR19973070.2522680/150‑1 (MQ=60)
                                 TAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATT                                                                                                                 <  SRR19973070.2277302/150‑1 (MQ=60)
                                 TAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATT                                                                                                                 >  SRR19973070.1199122/1‑150 (MQ=60)
                                          GAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCTGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCAT                                                                                                        <  SRR19973070.1947172/150‑1 (MQ=60)
                                          GAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCTGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCAT                                                                                                        <  SRR19973070.1947176/150‑1 (MQ=60)
                                           AGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCTGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATT                                                                                                       <  SRR19973070.2457546/150‑1 (MQ=60)
                                              TAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATG                                                                                                    >  SRR19973070.1995953/1‑150 (MQ=60)
                                                      GTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGT                                                                                            <  SRR19973070.1943834/150‑1 (MQ=60)
                                                        TTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTA                                                                                          <  SRR19973070.2239230/150‑1 (MQ=60)
                                                         TTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTAT                                                                                         <  SRR19973070.3193583/150‑1 (MQ=60)
                                                          TCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATT                                                                                        <  SRR19973070.2513436/150‑1 (MQ=60)
                                                           CCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTG                                                                                       <  SRR19973070.3314440/150‑1 (MQ=60)
                                                           CCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTG                                                                                       <  SRR19973070.3314379/150‑1 (MQ=60)
                                                           CCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGGGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTG                                                                                       <  SRR19973070.3314435/150‑1 (MQ=60)
                                                                 AACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCC                                                                                 <  SRR19973070.1795045/150‑1 (MQ=60)
                                                                    CTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCT                                                                              <  SRR19973070.282075/150‑1 (MQ=60)
                                                                    CTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCT                                                                              >  SRR19973070.1407591/1‑150 (MQ=60)
                                                                    CTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCT                                                                              <  SRR19973070.2609169/150‑1 (MQ=60)
                                                                     TTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTT                                                                             <  SRR19973070.197048/150‑1 (MQ=60)
                                                                      TGATGGGCCTGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTT                                                                            >  SRR19973070.1608826/1‑150 (MQ=60)
                                                                             CCTGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAA                                                                     <  SRR19973070.1234481/150‑1 (MQ=60)
                                                                             CCTGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAA                                                                     <  SRR19973070.1234470/150‑1 (MQ=60)
                                                                              CAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAAC                                                                    >  SRR19973070.3099117/1‑150 (MQ=60)
                                                                                  CGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAA                                                                <  SRR19973070.2171260/150‑1 (MQ=60)
                                                                                  CGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAA                                                                >  SRR19973070.89158/1‑150 (MQ=60)
                                                                                      AAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAAC                                                            <  SRR19973070.256911/150‑1 (MQ=60)
                                                                                       AAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACC                                                           >  SRR19973070.2952733/1‑150 (MQ=60)
                                                                                          TTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATT                                                        <  SRR19973070.1959938/150‑1 (MQ=60)
                                                                                            AGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTAC                                                      <  SRR19973070.180100/150‑1 (MQ=60)
                                                                                              AAAAATCACCGTAGTCATCGCTGGTGCTGTAGAACTTTATGATGGTTACTTGCATTACGTGTATGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGAGGTGATAGCGAGCCGCTTTCAAGAAACAGAAAAACCATTACaa                                                    >  SRR19973070.2942046/1‑148 (MQ=60)
                                                                                                AAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCT                                                  <  SRR19973070.2679067/150‑1 (MQ=60)
                                                                                                AAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCT                                                  >  SRR19973070.1632306/1‑150 (MQ=60)
                                                                                                      CCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAAC                                            >  SRR19973070.42735/1‑150 (MQ=60)
                                                                                                       CGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACC                                           <  SRR19973070.1369446/150‑1 (MQ=60)
                                                                                                       CGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACC                                           <  SRR19973070.1369593/150‑1 (MQ=60)
                                                                                                           CTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAA                                       >  SRR19973070.2222593/1‑150 (MQ=60)
                                                                                                            TCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAA                                      <  SRR19973070.700406/150‑1 (MQ=60)
                                                                                                             CATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAA                                     <  SRR19973070.2185578/150‑1 (MQ=60)
                                                                                                              ATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAAT                                    >  SRR19973070.7630/1‑150 (MQ=60)
                                                                                                              ATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAAT                                    >  SRR19973070.1399105/1‑150 (MQ=60)
                                                                                                              ATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAAT                                    <  SRR19973070.1911549/150‑1 (MQ=60)
                                                                                                              ATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAAT                                    >  SRR19973070.328952/1‑150 (MQ=60)
                                                                                                                   TGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCAC                               >  SRR19973070.1791213/1‑150 (MQ=60)
                                                                                                                   TGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCAC                               >  SRR19973070.1791215/1‑150 (MQ=60)
                                                                                                                    GGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACA                              <  SRR19973070.340958/150‑1 (MQ=60)
                                                                                                                    GGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACA                              <  SRR19973070.340663/150‑1 (MQ=60)
                                                                                                                     GTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAA                             <  SRR19973070.2007387/150‑1 (MQ=60)
                                                                                                                     GTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAA                             >  SRR19973070.2750386/1‑150 (MQ=60)
                                                                                                                     GTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAA                             <  SRR19973070.1578054/150‑1 (MQ=60)
                                                                                                                     GTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTCGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAA                             >  SRR19973070.2751719/1‑150 (MQ=60)
                                                                                                                            AGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGC                      <  SRR19973070.2958646/150‑1 (MQ=60)
                                                                                                                               AATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGT                   <  SRR19973070.1626788/150‑1 (MQ=60)
                                                                                                                                 TTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTT                 <  SRR19973070.752983/150‑1 (MQ=60)
                                                                                                                                   TATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTAT               <  SRR19973070.311245/150‑1 (MQ=60)
                                                                                                                                     TGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATAC             >  SRR19973070.1230835/1‑150 (MQ=60)
                                                                                                                                     TGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATAC             <  SRR19973070.1095648/150‑1 (MQ=60)
                                                                                                                                     TGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATAC             <  SRR19973070.1095600/150‑1 (MQ=60)
                                                                                                                                           TGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATT       <  SRR19973070.1535548/150‑1 (MQ=60)
                                                                                                                                                TGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGG  <  SRR19973070.2761877/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                
TATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGG  >  CP009273/828225‑828518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑