Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 828,374 G→T 91.6% V11V (GTC→GTA)  ybiA ← DUF1768 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273828,3740GT91.6% 656.5 / 47.5 215V11V (GTC→GTA) ybiADUF1768 family protein
Reads supporting (aligned to +/- strand):  ref base G (11/7);  new base T (109/88);  total (120/95)
Fisher's exact test for biased strand distribution p-value = 8.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 216 total aligned reads displayed.

TTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGGTTTTC  >  CP009273/828229‑828523
                                                                                                                                                 |                                                                                                                                                     
       aaGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGAt                                                                                                                                            <  1:2838355/150‑1 (MQ=255)
       aaGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGAt                                                                                                                                            >  1:3865020/1‑150 (MQ=255)
        aGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATc                                                                                                                                           <  1:3087048/150‑1 (MQ=255)
        aGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATc                                                                                                                                           <  1:3751385/150‑1 (MQ=255)
         ggAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATcc                                                                                                                                          <  1:1669405/150‑1 (MQ=255)
         ggAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATcc                                                                                                                                          <  1:3857724/150‑1 (MQ=255)
           aaTTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCtt                                                                                                                                        <  2:2358437/150‑1 (MQ=255)
           aaTTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCtg                                                                                                                                        >  2:3474580/1‑149 (MQ=255)
            aTTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGACAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCttt                                                                                                                                       >  1:1066131/1‑150 (MQ=255)
                  gtgCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTc                                                                                                                                 <  1:2256157/150‑1 (MQ=255)
                  gtgCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTc                                                                                                                                 >  1:3237590/1‑150 (MQ=255)
                  gtgCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTc                                                                                                                                 >  1:3448231/1‑150 (MQ=255)
                    gCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGa                                                                                                                               <  1:3656298/150‑1 (MQ=255)
                    gCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGa                                                                                                                               <  2:2672409/150‑1 (MQ=255)
                      cTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAAc                                                                                                                             >  2:358449/1‑150 (MQ=255)
                       tGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACg                                                                                                                            >  2:3818545/1‑150 (MQ=255)
                         aaaaTAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACggg                                                                                                                          <  2:1044500/150‑1 (MQ=255)
                         aaaaTAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACggg                                                                                                                          >  1:3119580/1‑150 (MQ=255)
                          aaaTAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGc                                                                                                                         >  2:1387800/1‑150 (MQ=255)
                          aaaTAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGc                                                                                                                         >  2:1374116/1‑150 (MQ=255)
                           aaTAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCa                                                                                                                        >  1:766152/1‑150 (MQ=255)
                            aTAGTGTTCTGAGGTAGGCCTGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCATAGTTATAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCAt                                                                                                                       <  1:2263115/150‑1 (MQ=255)
                              aGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTa                                                                                                                     >  2:163219/1‑150 (MQ=255)
                                  ttCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAAcc                                                                                                                 <  2:438002/150‑1 (MQ=255)
                                    cTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGc                                                                                                               >  1:1986873/1‑150 (MQ=255)
                                     tGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCa                                                                                                              >  2:960498/1‑150 (MQ=255)
                                     tGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCa                                                                                                              >  2:1673419/1‑150 (MQ=255)
                                      gAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCAt                                                                                                             >  1:1875142/1‑150 (MQ=255)
                                      gAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCAt                                                                                                             >  1:3310203/1‑150 (MQ=255)
                                       aGGTAGGCCAGGTTGTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCAtt                                                                                                            <  2:2460747/150‑1 (MQ=255)
                                        ggTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTa                                                                                                           >  2:1508638/1‑150 (MQ=255)
                                           aGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGt                                                                                                        >  2:4272811/1‑150 (MQ=255)
                                               cAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTtggt                                                                                                    >  1:1124690/1‑150 (MQ=255)
                                                aGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTtggtg                                                                                                   >  1:714571/1‑150 (MQ=255)
                                                aGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTtggtg                                                                                                   >  1:1226592/1‑150 (MQ=255)
                                                  gTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTggtggt                                                                                                 <  1:3196729/150‑1 (MQ=255)
                                                    tttCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTa                                                                                               >  1:1445054/1‑150 (MQ=255)
                                                    tttCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTa                                                                                               <  1:3510195/150‑1 (MQ=255)
                                                      tCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTAtt                                                                                             <  1:2816040/150‑1 (MQ=255)
                                                      tCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTAtt                                                                                             <  2:1031543/150‑1 (MQ=255)
                                                         cGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCCTTATGTTGGTGGTTATTGCg                                                                                          >  2:3261812/1‑150 (MQ=255)
                                                          gTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGa                                                                                         <  1:1560410/150‑1 (MQ=255)
                                                           tCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAg                                                                                        <  1:2241568/150‑1 (MQ=255)
                                                             aaCCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGcc                                                                                      >  1:243985/1‑150 (MQ=255)
                                                             aaCCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGcc                                                                                      >  1:3580260/1‑150 (MQ=255)
                                                                 ttGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCtt                                                                                  >  1:3718552/1‑150 (MQ=255)
                                                                  tGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCttt                                                                                 >  1:2737801/1‑150 (MQ=255)
                                                                  tGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCttt                                                                                 <  1:3215955/150‑1 (MQ=255)
                                                                  tGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCttt                                                                                 <  2:2638941/150‑1 (MQ=255)
                                                                      gggCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCcag                                                                             <  2:1777144/150‑1 (MQ=255)
                                                                      gggCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCcag                                                                             <  1:180288/150‑1 (MQ=255)
                                                                         ccAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCcagaaa                                                                          >  2:3093000/1‑150 (MQ=255)
                                                                           aGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCcagaaaca                                                                        >  1:1383946/1‑150 (MQ=255)
                                                                           aGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCcagaaaca                                                                        <  1:950813/150‑1 (MQ=255)
                                                                           aGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCcagaaaca                                                                        >  2:1165914/1‑150 (MQ=255)
                                                                             gcggcAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCcagaaacaga                                                                      <  1:1314983/150‑1 (MQ=255)
                                                                              cggcAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCcagaaacagaa                                                                     >  2:408501/1‑150 (MQ=255)
                                                                               ggcAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCcagaaacagaaa                                                                    >  2:3691458/1‑150 (MQ=255)
                                                                                gcAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGaaaa                                                                   >  2:1249492/1‑150 (MQ=255)
                                                                                 cAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGaaaaa                                                                  >  2:4215453/1‑150 (MQ=255)
                                                                                  aaaGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAAc                                                                 <  2:3524231/150‑1 (MQ=255)
                                                                                    aGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTGTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCa                                                               <  2:1416865/150‑1 (MQ=255)
                                                                                     gTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCAt                                                              <  1:3187535/150‑1 (MQ=255)
                                                                                      ttAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCAtt                                                             <  1:1035036/150‑1 (MQ=255)
                                                                                      ttAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCAtt                                                             <  1:4257073/150‑1 (MQ=255)
                                                                                      ttAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCAtt                                                             <  1:3918475/150‑1 (MQ=255)
                                                                                        aGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTAc                                                           >  2:2297341/1‑150 (MQ=255)
                                                                                         gAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTAcc                                                          >  2:1973813/1‑150 (MQ=255)
                                                                                             aaTCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTg                                                      <  2:1149256/150‑1 (MQ=255)
                                                                                                  ccGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAAc                                                 <  2:2946997/150‑1 (MQ=255)
                                                                                                   cGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAAcc                                                >  1:321091/1‑150 (MQ=255)
                                                                                                       gTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGaaa                                            >  1:550393/1‑150 (MQ=255)
                                                                                                        tCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGATGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGaaaa                                           <  1:607174/150‑1 (MQ=255)
                                                                                                          aTCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAAt                                         >  1:532324/1‑150 (MQ=255)
                                                                                                          aTCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAAt                                         >  1:2396295/1‑150 (MQ=255)
                                                                                                            cGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGc                                       <  1:821618/150‑1 (MQ=255)
                                                                                                               tGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCcac                                    >  1:2857898/1‑150 (MQ=255)
                                                                                                                ggTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCcaca                                   <  2:2083482/150‑1 (MQ=255)
                                                                                                                ggTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCcaca                                   <  2:1470481/150‑1 (MQ=255)
                                                                                                                   gCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAata                                >  1:4094664/1‑150 (MQ=255)
                                                                                                                     tGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATAtt                              >  2:495964/1‑150 (MQ=255)
                                                                                                                     tGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATAtt                              <  1:2685147/150‑1 (MQ=255)
                                                                                                                      gTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTg                             >  1:631072/1‑150 (MQ=255)
                                                                                                                      gTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTg                             >  2:2291351/1‑150 (MQ=255)
                                                                                                                      gTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCAGTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTg                             >  2:765802/1‑150 (MQ=255)
                                                                                                                        aGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGc                           >  2:2102661/1‑150 (MQ=255)
                                                                                                                        aGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGc                           >  2:392657/1‑150 (MQ=255)
                                                                                                                         gAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCt                          >  2:1056221/1‑150 (MQ=255)
                                                                                                                            aTTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGtt                       >  1:3909533/1‑150 (MQ=255)
                                                                                                                                atgatgGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTtata                   >  1:2467574/1‑150 (MQ=255)
                                                                                                                                atgatgGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTtata                   >  1:349396/1‑150 (MQ=255)
                                                                                                                                 tgatgGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATAc                  >  2:4168989/1‑150 (MQ=255)
                                                                                                                                  gatgGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACa                 <  2:3712541/150‑1 (MQ=255)
                                                                                                                                  gatgGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACa                 >  2:2797874/1‑150 (MQ=255)
                                                                                                                                   atgGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAg                <  2:715005/150‑1 (MQ=255)
                                                                                                                                     ggTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTa              >  1:2358429/1‑150 (MQ=255)
                                                                                                                                        gtCTTGCATTACGGGTTGGATCCATTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTAttt           <  2:3047563/150‑1 (MQ=255)
                                                                                                                                           ttGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAg        >  1:2865060/1‑150 (MQ=255)
                                                                                                                                           ttGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAg        >  2:2975287/1‑150 (MQ=255)
                                                                                                                                                 gACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGGTTTtc  <  2:3865020/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
TTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGGTTTTC  >  CP009273/828229‑828523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGGTTT  >  CP009273/828229‑828521
                                                                                                                                                 |                                                                                                                                                   
TTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGT                                                                                                                                                 <  SRR19973079.811217/150‑1 (MQ=60)
TTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGT                                                                                                                                                 >  SRR19973079.2028389/1‑150 (MQ=60)
    ATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTG                                                                                                                                             >  SRR19973079.3184607/1‑150 (MQ=60)
     TCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGG                                                                                                                                            >  SRR19973079.3965326/1‑150 (MQ=60)
       AAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGAT                                                                                                                                          <  SRR19973079.2852807/150‑1 (MQ=60)
       AAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGAT                                                                                                                                          <  SRR19973079.2852826/150‑1 (MQ=60)
       AAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGAT                                                                                                                                          >  SRR19973079.3884695/1‑150 (MQ=60)
        AGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATC                                                                                                                                         <  SRR19973079.3103014/150‑1 (MQ=60)
        AGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATC                                                                                                                                         <  SRR19973079.3103021/150‑1 (MQ=60)
        AGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATC                                                                                                                                         <  SRR19973079.3770602/150‑1 (MQ=60)
         GGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCC                                                                                                                                        <  SRR19973079.3877385/150‑1 (MQ=60)
         GGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCC                                                                                                                                        <  SRR19973079.1678426/150‑1 (MQ=60)
         GGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCC                                                                                                                                        <  SRR19973079.3877353/150‑1 (MQ=60)
            ATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTT                                                                                                                                     <  SRR19973079.1398151/150‑1 (MQ=60)
            ATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGACAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTT                                                                                                                                     >  SRR19973079.1071949/1‑150 (MQ=60)
                  GTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTC                                                                                                                               >  SRR19973079.1048310/1‑150 (MQ=60)
                  GTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTC                                                                                                                               <  SRR19973079.2267854/150‑1 (MQ=60)
                  GTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTC                                                                                                                               >  SRR19973079.3253992/1‑150 (MQ=60)
                  GTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTC                                                                                                                               >  SRR19973079.3466028/1‑150 (MQ=60)
                  GTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTC                                                                                                                               >  SRR19973079.3467081/1‑150 (MQ=60)
                    GCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGA                                                                                                                             <  SRR19973079.3674909/150‑1 (MQ=60)
                      CTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAAC                                                                                                                           <  SRR19973079.3463579/150‑1 (MQ=60)
                       TGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACG                                                                                                                          <  SRR19973079.3704285/150‑1 (MQ=60)
                        GAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGG                                                                                                                         <  SRR19973079.159109/150‑1 (MQ=60)
                         AAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGG                                                                                                                        >  SRR19973079.3135658/1‑150 (MQ=60)
                         AAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGG                                                                                                                        >  SRR19973079.568392/1‑150 (MQ=60)
                           AATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGTCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCA                                                                                                                      >  SRR19973079.3744593/1‑150 (MQ=60)
                           AATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCA                                                                                                                      >  SRR19973079.770407/1‑150 (MQ=60)
                            ATAGTGTTCTGAGGTAGGCCTGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCATAGTTATAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCAT                                                                                                                     <  SRR19973079.2274859/150‑1 (MQ=60)
                            ATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCAT                                                                                                                     >  SRR19973079.638308/1‑150 (MQ=60)
                            ATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCAT                                                                                                                     <  SRR19973079.2274639/150‑1 (MQ=60)
                                    CTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGC                                                                                                             >  SRR19973079.988024/1‑150 (MQ=60)
                                    CTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGC                                                                                                             >  SRR19973079.1997413/1‑150 (MQ=60)
                                      GAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCAT                                                                                                           >  SRR19973079.699854/1‑150 (MQ=60)
                                      GAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCAT                                                                                                           >  SRR19973079.1885110/1‑150 (MQ=60)
                                      GAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCAT                                                                                                           >  SRR19973079.3327104/1‑150 (MQ=60)
                                               CAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGT                                                                                                  >  SRR19973079.1130901/1‑150 (MQ=60)
                                               CAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGT                                                                                                  >  SRR19973079.1131092/1‑150 (MQ=60)
                                                AGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTG                                                                                                 >  SRR19973079.1233636/1‑150 (MQ=60)
                                                AGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTG                                                                                                 >  SRR19973079.1233544/1‑150 (MQ=60)
                                                AGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTG                                                                                                 >  SRR19973079.718833/1‑150 (MQ=60)
                                                AGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTG                                                                                                 >  SRR19973079.718399/1‑150 (MQ=60)
                                                  GTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGT                                                                                               <  SRR19973079.3213053/150‑1 (MQ=60)
                                                  GTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGT                                                                                               <  SRR19973079.3212638/150‑1 (MQ=60)
                                                   TTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTT                                                                                              >  SRR19973079.2548613/1‑150 (MQ=60)
                                                    TTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTA                                                                                             >  SRR19973079.1452851/1‑150 (MQ=60)
                                                    TTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTA                                                                                             <  SRR19973079.3528245/150‑1 (MQ=60)
                                                     TTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTAT                                                                                            <  SRR19973079.2730889/150‑1 (MQ=60)
                                                      TCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATT                                                                                           <  SRR19973079.2830450/150‑1 (MQ=60)
                                                      TCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATT                                                                                           <  SRR19973079.3042002/150‑1 (MQ=60)
                                                      TCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATT                                                                                           <  SRR19973079.1839104/150‑1 (MQ=60)
                                                          GTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGA                                                                                       <  SRR19973079.1257040/150‑1 (MQ=60)
                                                          GTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGA                                                                                       <  SRR19973079.1569024/150‑1 (MQ=60)
                                                           TCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAG                                                                                      <  SRR19973079.2253172/150‑1 (MQ=60)
                                                           TCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAG                                                                                      <  SRR19973079.1929156/150‑1 (MQ=60)
                                                             AACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCC                                                                                    >  SRR19973079.3598496/1‑150 (MQ=60)
                                                             AACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCC                                                                                    >  SRR19973079.245603/1‑150 (MQ=60)
                                                             AACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCC                                                                                    >  SRR19973079.2433114/1‑150 (MQ=60)
                                                             AACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTGTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCC                                                                                    <  SRR19973079.2475699/150‑1 (MQ=60)
                                                              ACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCG                                                                                   <  SRR19973079.2299070/150‑1 (MQ=60)
                                                                 TTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTT                                                                                >  SRR19973079.3737585/1‑150 (MQ=60)
                                                                 TTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTGTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTT                                                                                <  SRR19973079.3877442/150‑1 (MQ=60)
                                                                  TGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTT                                                                               <  SRR19973079.3231991/150‑1 (MQ=60)
                                                                  TGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTT                                                                               <  SRR19973079.3232322/150‑1 (MQ=60)
                                                                  TGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTT                                                                               >  SRR19973079.2751944/1‑150 (MQ=60)
                                                                   GATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTC                                                                              >  SRR19973079.3526354/1‑150 (MQ=60)
                                                                      GGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAG                                                                           <  SRR19973079.2687222/150‑1 (MQ=60)
                                                                      GGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAG                                                                           <  SRR19973079.181625/150‑1 (MQ=60)
                                                                       GGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGA                                                                          >  SRR19973079.2254452/1‑150 (MQ=60)
                                                                       GGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGA                                                                          >  SRR19973079.2255346/1‑150 (MQ=60)
                                                                           AGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACA                                                                      <  SRR19973079.956263/150‑1 (MQ=60)
                                                                           AGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACA                                                                      >  SRR19973079.1391581/1‑150 (MQ=60)
                                                                           AGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACA                                                                      >  SRR19973079.1391588/1‑150 (MQ=60)
                                                                             GCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGA                                                                    <  SRR19973079.1322412/150‑1 (MQ=60)
                                                                                  AAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAAC                                                               >  SRR19973079.4230700/1‑150 (MQ=60)
                                                                                   AAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACC                                                              >  SRR19973079.3607716/1‑150 (MQ=60)
                                                                                     GTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCAT                                                            <  SRR19973079.3203820/150‑1 (MQ=60)
                                                                                     GTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCAT                                                            <  SRR19973079.3324852/150‑1 (MQ=60)
                                                                                      TTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATT                                                           <  SRR19973079.4278604/150‑1 (MQ=60)
                                                                                      TTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATT                                                           <  SRR19973079.407635/150‑1 (MQ=60)
                                                                                      TTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATT                                                           <  SRR19973079.1040779/150‑1 (MQ=60)
                                                                                      TTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATT                                                           <  SRR19973079.3590453/150‑1 (MQ=60)
                                                                                      TTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATT                                                           <  SRR19973079.3938310/150‑1 (MQ=60)
                                                                                      TTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATT                                                           <  SRR19973079.3938156/150‑1 (MQ=60)
                                                                                              ATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGA                                                   <  SRR19973079.1494266/150‑1 (MQ=60)
                                                                                                  CCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTGTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAAC                                               <  SRR19973079.3215809/150‑1 (MQ=60)
                                                                                                   CGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCGCTGAAAACC                                              <  SRR19973079.4339553/150‑1 (MQ=60)
                                                                                                   CGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACC                                              >  SRR19973079.322923/1‑150 (MQ=60)
                                                                                                       GTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAA                                          >  SRR19973079.553685/1‑150 (MQ=60)
                                                                                                       GTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAA                                          >  SRR19973079.2372895/1‑150 (MQ=60)
                                                                                                        TCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAA                                         <  SRR19973079.2991909/150‑1 (MQ=60)
                                                                                                        TCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGATGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAA                                         <  SRR19973079.610704/150‑1 (MQ=60)
                                                                                                          ATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAAT                                       >  SRR19973079.2408680/1‑150 (MQ=60)
                                                                                                          ATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAAT                                       >  SRR19973079.535608/1‑150 (MQ=60)
                                                                                                          ATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAAT                                       >  SRR19973079.535530/1‑150 (MQ=60)
                                                                                                          ATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAAAAGAAAAACCATTACACATGAAAACCGAAAAAT                                       >  SRR19973079.2768389/1‑150 (MQ=60)
                                                                                                            CGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGC                                     <  SRR19973079.826352/150‑1 (MQ=60)
                                                                                                               TGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCAC                                  >  SRR19973079.2873233/1‑150 (MQ=60)
                                                                                                               TGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCAC                                  >  SRR19973079.2872202/1‑150 (MQ=60)
                                                                                                               TGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCAC                                  >  SRR19973079.2872419/1‑150 (MQ=60)
                                                                                                                   GCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATA                              >  SRR19973079.4115418/1‑150 (MQ=60)
                                                                                                                     TGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATT                            <  SRR19973079.2699053/150‑1 (MQ=60)
                                                                                                                      GTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTG                           >  SRR19973079.634689/1‑150 (MQ=60)
                                                                                                                      GTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTG                           >  SRR19973079.633507/1‑150 (MQ=60)
                                                                                                                       TAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGG                          >  SRR19973079.3281795/1‑150 (MQ=60)
                                                                                                                        AGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTGTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGC                         <  SRR19973079.1472136/150‑1 (MQ=60)
                                                                                                                        AGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCGTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGC                         <  SRR19973079.3238826/150‑1 (MQ=60)
                                                                                                                            ATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTT                     >  SRR19973079.1728313/1‑150 (MQ=60)
                                                                                                                            ATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTT                     >  SRR19973079.3929346/1‑150 (MQ=60)
                                                                                                                                ATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATA                 >  SRR19973079.351297/1‑150 (MQ=60)
                                                                                                                                ATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATA                 >  SRR19973079.2480192/1‑150 (MQ=60)
                                                                                                                                 TGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATAC                <  SRR19973079.3890995/150‑1 (MQ=60)
                                                                                                                                     GGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTA            >  SRR19973079.2370658/1‑150 (MQ=60)
                                                                                                                                        GTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACAGAAAAATGACACAATATTGGCTGTTTATACAGTATTT         >  SRR19973079.842236/1‑150 (MQ=60)
                                                                                                                                           TTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAG      <  SRR19973079.369674/150‑1 (MQ=60)
                                                                                                                                           TTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAG      >  SRR19973079.2879602/1‑150 (MQ=60)
                                                                                                                                               ATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGGTTT  >  SRR19973079.461398/1‑150 (MQ=60)
                                                                                                                                                 |                                                                                                                                                   
TTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGGTTT  >  CP009273/828229‑828521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑