Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F309 I0 R1	1697	197.2	6627756	95.7%	6342762	147.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	850,161	G→T	87.1%	P353P (CCG→CCT)	ybiR →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	850,161	0	G	T	87.1%	299.8 / 42.2	141	P353P (CCG→CCT)	ybiR	putative transporter
Reads supporting (aligned to +/- strand): ref base G (8/11); new base T (58/64); total (66/75)
Fisher's exact test for biased strand distribution p-value = 8.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 141 total aligned reads displayed.

CTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGG  >  CP009273/850017‑850305

   tgtgCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCtg                                                                                                                                            <  2:2042761/148‑1 (MQ=255)

       ccgccgTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCtgttgt                                                                                                                                        <  2:1845191/148‑1 (MQ=255)

             tCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGggcgg                                                                                                                                  >  2:497089/1‑148 (MQ=255)

             tCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGggcgg                                                                                                                                  >  2:2474154/1‑148 (MQ=255)

              cTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGggcggc                                                                                                                                 >  1:2079905/1‑148 (MQ=255)

              cTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGggcggc                                                                                                                                 >  2:2535630/1‑148 (MQ=255)

              cTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGggcggc                                                                                                                                 >  2:2455935/1‑148 (MQ=255)

               tttATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGgcggcg                                                                                                                                <  2:1242467/148‑1 (MQ=255)

               tttATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGAATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGgcggcg                                                                                                                                >  2:2035897/1‑148 (MQ=255)

                ttattaCTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGt                                                                                                                               <  1:1092131/148‑1 (MQ=255)

                  attaCTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTtg                                                                                                                             <  1:2091126/148‑1 (MQ=255)

                  attaCTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTtg                                                                                                                             <  1:1540324/148‑1 (MQ=255)

                          tATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGata                                                                                                                     <  2:2272739/148‑1 (MQ=255)

                          tATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGata                                                                                                                     >  1:1864502/1‑148 (MQ=255)

                          tATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGata                                                                                                                     >  2:467444/1‑148 (MQ=255)

                          tATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATAACTATGCTGTTGTGGGCGGCGTTGGTGGGcta                                                                                                                     >  1:46316/1‑148 (MQ=255)

                          tATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTGTCCATCTCTATTCAAAACCTATGCTGGTGTGGGCGGCGTTGGTGGGata                                                                                                                     >  1:46330/1‑148 (MQ=255)

                              ggCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGtt                                                                                                                 <  2:483898/148‑1 (MQ=255)

                               gCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGttt                                                                                                                <  1:2353861/148‑1 (MQ=255)

                                cGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGtttt                                                                                                               <  2:3046549/148‑1 (MQ=255)

                                 ggTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTg                                                                                                              <  2:3250100/148‑1 (MQ=255)

                                 ggTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTg                                                                                                              <  2:1707439/148‑1 (MQ=255)

                                  gTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGGTTTgt                                                                                                             >  1:1007800/1‑148 (MQ=255)

                                   tAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTgtt                                                                                                            >  1:3247008/1‑148 (MQ=255)

                                      aCGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTgttagt                                                                                                         >  2:2965312/1‑148 (MQ=255)

                                      aCGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTgttagt                                                                                                         >  2:541433/1‑148 (MQ=255)

                                          aGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTtata                                                                                                     <  1:243139/148‑1 (MQ=255)

                                           ggTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATAc                                                                                                    >  1:608104/1‑148 (MQ=255)

                                             tGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTc                                                                                                  <  2:922074/148‑1 (MQ=255)

                                                cTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCg                                                                                               >  2:3209975/1‑148 (MQ=255)

                                                    gggTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCa                                                                                           <  1:562964/148‑1 (MQ=255)

                                                       ttattaCCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACt                                                                                        >  1:493352/1‑148 (MQ=255)

                                                       ttattaCCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACt                                                                                        >  1:3275377/1‑148 (MQ=255)

                                                       ttattaCCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACt                                                                                        <  1:1293044/148‑1 (MQ=255)

                                                       ttattaCCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACt                                                                                        >  2:1375486/1‑148 (MQ=255)

                                                        tattaCCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTa                                                                                       >  2:3209863/1‑148 (MQ=255)

                                                        tattaCCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGGTATACTCCCGGCCAACTa                                                                                       >  2:2623891/1‑148 (MQ=255)

                                                          ttaCCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAgg                                                                                     <  2:639663/148‑1 (MQ=255)

                                                              ccGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCt                                                                                 >  1:3190008/1‑148 (MQ=255)

                                                              ccGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTAAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCt                                                                                 >  1:802210/1‑148 (MQ=255)

                                                               cGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTg                                                                                <  1:2188221/148‑1 (MQ=255)

                                                               cGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTg                                                                                <  2:2457673/148‑1 (MQ=255)

                                                               cGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTg                                                                                <  1:24985/148‑1 (MQ=255)

                                                                  aTCGCTGGCAAATTTGATTGCGATACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCa                                                                             >  2:1425221/1‑148 (MQ=255)

                                                                   tCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCaa                                                                            <  2:154654/148‑1 (MQ=255)

                                                                   tCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCaa                                                                            >  1:101533/1‑148 (MQ=255)

                                                                    cGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCaaa                                                                           <  2:610705/148‑1 (MQ=255)

                                                                       tGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATaa                                                                        <  2:2814210/148‑1 (MQ=255)

                                                                       tGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATaa                                                                        <  2:2869423/148‑1 (MQ=255)

                                                                         gCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATaaaa                                                                      >  2:1325197/1‑148 (MQ=255)

                                                                          cAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATaaaaa                                                                     >  2:965732/1‑148 (MQ=255)

                                                                           aaaTTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATaaaaaa                                                                    <  1:2535630/148‑1 (MQ=255)

                                                                             aTTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAgg                                                                  >  1:477486/1‑148 (MQ=255)

                                                                             aTTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAgg                                                                  <  2:145611/148‑1 (MQ=255)

                                                                             aTTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAgg                                                                  <  2:145679/148‑1 (MQ=255)

                                                                              tttGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGc                                                                 >  2:1948978/1‑148 (MQ=255)

                                                                                tGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCgg                                                               <  1:1223538/148‑1 (MQ=255)

                                                                                tGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCgg                                                               <  1:1223570/148‑1 (MQ=255)

                                                                                 gATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGa                                                              <  2:3247008/148‑1 (MQ=255)

                                                                                   ttGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGAtt                                                            <  1:1437161/148‑1 (MQ=255)

                                                                                    tGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTa                                                           <  2:2217505/148‑1 (MQ=255)

                                                                                    tGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTa                                                           <  1:2615102/148‑1 (MQ=255)

                                                                                      cgcTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATg                                                         <  1:1646576/148‑1 (MQ=255)

                                                                                      cgcTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATg                                                         <  1:802723/148‑1 (MQ=255)

                                                                                      cgcTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATg                                                         <  2:1524478/148‑1 (MQ=255)

                                                                                        cTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAg                                                       <  2:412719/148‑1 (MQ=255)

                                                                                                    aaCGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGc                                           <  1:1243684/148‑1 (MQ=255)

                                                                                                     aCGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCt                                          <  2:3098190/148‑1 (MQ=255)

                                                                                                     aCGATCGCCGCATCTGGTGGCGTTTCCATCTCGATTCAATACCTATGCGGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCCACTAGGGTCTGGCAAATAAAAAAGGCGGATTATTATTCCGCCGTTTTCt                                          >  1:2263378/1‑148 (MQ=255)

                                                                                                     aCGATCGCCGCATCTGGTGGCGTGTCCATCTCTATTAAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGCTATGTTTTTTTATTTATACTCCCGGCCAACTAGGGTCTGGCCAATAAAAAAGGCGGATTATTATTCCGCCTTTTTCt                                          >  1:2263390/1‑148 (MQ=255)

                                                                                                      cGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCtt                                         >  2:1785981/1‑148 (MQ=255)

                                                                                                        aTCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTa                                       <  1:2684044/148‑1 (MQ=255)

                                                                                                          cgccgcATCTGGTGGCGTTTCCATCTCTATTCAATACCTCTGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAg                                     <  2:1045135/148‑1 (MQ=255)

                                                                                                          cgccgcATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAg                                     <  2:2735379/148‑1 (MQ=255)

                                                                                                              gcATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGtttt                                 >  1:1809180/1‑148 (MQ=255)

                                                                                                               cATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTg                                <  1:1447837/148‑1 (MQ=255)

                                                                                                                aTCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGa                               <  1:3049394/148‑1 (MQ=255)

                                                                                                                 tCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAAAAAAAAATGCGGATTATTAGTCCGCCTTTTGCTTTAAGTTTTTaa                              >  1:139353/1‑148 (MQ=255)

                                                                                                                  cTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTGGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAg                             <  1:2048145/148‑1 (MQ=255)

                                                                                                                  cTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAg                             <  1:3240198/148‑1 (MQ=255)

                                                                                                                   tggtggCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGa                            <  1:2733761/148‑1 (MQ=255)

                                                                                                                   tggtggCGTTTCCATCTCTATTCAAGACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGa                            <  2:307874/148‑1 (MQ=255)

                                                                                                                    ggtggCGTTTCCATCTCTATTAAATACCTATGGTGTTTTGGGCGGCGTTGGTGGGTGATGTTTTGTTAGTTATACTCCCGGCCAAATAGGGTCTGGCAAATAAAAAAGGAGTATTATGAGTACGCCTTTTGCTTTAAGTTTTGAAGAt                           >  2:2029098/1‑148 (MQ=255)

                                                                                                                       ggCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTaa                        <  1:2473645/148‑1 (MQ=255)

                                                                                                                        gCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAAt                       <  2:840127/148‑1 (MQ=255)

                                                                                                                        gCGTGTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAAt                       <  2:840245/148‑1 (MQ=255)

                                                                                                                        gCGGTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAAt                       <  2:2212805/148‑1 (MQ=255)

                                                                                                                         cGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAAtt                      <  2:818232/148‑1 (MQ=255)

                                                                                                                         cGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAAtt                      <  2:2286326/148‑1 (MQ=255)

                                                                                                                           tttCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCa                    >  2:2683469/1‑148 (MQ=255)

                                                                                                                           tttCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCa                    >  1:3278845/1‑148 (MQ=255)

                                                                                                                              ccATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGTAGATTAATTCAGAc                 >  1:2161373/1‑148 (MQ=255)

                                                                                                                                aTCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGa               >  2:2501357/1‑148 (MQ=255)

                                                                                                                                 tctcTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGaa              <  2:2597128/148‑1 (MQ=255)

                                                                                                                                   tctATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAAcc            >  1:2884347/1‑148 (MQ=255)

                                                                                                                                   tctATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAAcc            >  1:1635865/1‑148 (MQ=255)

                                                                                                                                      aTTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGc         <  1:958766/148‑1 (MQ=255)

                                                                                                                                        tCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCAt       <  1:467444/148‑1 (MQ=255)

                                                                                                                                          aaTACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATcc     <  2:2260356/148‑1 (MQ=255)

                                                                                                                                            tACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCg   >  2:1764042/1‑148 (MQ=255)

CTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGG  >  CP009273/850017‑850305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCC  >  CP009273/850012‑850302

CTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCT                                                                                                                                               >  SRR19973070.2227352/1‑150 (MQ=60)

 TGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTA                                                                                                                                              <  SRR19973070.2407048/150‑1 (MQ=60)

   AACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATG                                                                                                                                            >  SRR19973070.2275353/1‑150 (MQ=60)

         GTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTG                                                                                                                                      >  SRR19973070.232900/1‑150 (MQ=60)

                 GTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGC                                                                                                                              >  SRR19973070.2093903/1‑150 (MQ=60)

                  TCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAAAACCGAAGCTGTTGTGGGCGGCG                                                                                                                             >  SRR19973070.1535183/1‑150 (MQ=60)

                     TTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTG                                                                                                                          <  SRR19973070.3326310/150‑1 (MQ=60)

                     TTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTG                                                                                                                          <  SRR19973070.1099673/150‑1 (MQ=60)

                      TATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGG                                                                                                                         <  SRR19973070.752866/150‑1 (MQ=60)

                       ATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGT                                                                                                                        <  SRR19973070.2105186/150‑1 (MQ=60)

                       ATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGT                                                                                                                        <  SRR19973070.1551149/150‑1 (MQ=60)

                             GGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATA                                                                                                                  >  SRR19973070.1877245/1‑150 (MQ=60)

                             GGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATAACTATGCTGTTGTGGGCGGCGTTGGTGGGCTA                                                                                                                  >  SRR19973070.46899/1‑150 (MQ=60)

                             GGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTGTCCATCTCTATTCAAAACCTATGCTGGTGTGGGCGGCGTTGGTGGGATA                                                                                                                  >  SRR19973070.46913/1‑150 (MQ=60)

                                 TGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTT                                                                                                              >  SRR19973070.552141/1‑150 (MQ=60)

                                    GCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTG                                                                                                           <  SRR19973070.2369454/150‑1 (MQ=60)

                                     CGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGGTTTGT                                                                                                          >  SRR19973070.1014974/1‑150 (MQ=60)

                                      GGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTT                                                                                                         >  SRR19973070.3267935/1‑150 (MQ=60)

                                      GGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTAAATAACGATGCTGTTGTGGGCGGCCTTGGTGTGATCTGTTTTGTT                                                                                                         >  SRR19973070.3267936/1‑150 (MQ=60)

                                              TAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGGTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATAC                                                                                                 >  SRR19973070.2583331/1‑150 (MQ=60)

                                              TAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTATTTATAC                                                                                                 >  SRR19973070.612364/1‑150 (MQ=60)

                                               AGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACT                                                                                                <  SRR19973070.244813/150‑1 (MQ=60)

                                                 GTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCC                                                                                              <  SRR19973070.2854283/150‑1 (MQ=60)

                                                  TGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCC                                                                                             <  SRR19973070.1240511/150‑1 (MQ=60)

                                                      TTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCC                                                                                         >  SRR19973070.3287676/1‑150 (MQ=60)

                                                         GGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAAC                                                                                      <  SRR19973070.566708/150‑1 (MQ=60)

                                                          GGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACT                                                                                     >  SRR19973070.3296427/1‑150 (MQ=60)

                                                          GGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACT                                                                                     >  SRR19973070.496801/1‑150 (MQ=60)

                                                            TTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAG                                                                                   <  SRR19973070.1302052/150‑1 (MQ=60)

                                                                TACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGGGGGCGGCGGTGGTGGGATATGTTTTGTTATTTATACTCCCGGCCAACTAGGGTC                                                                               >  SRR19973070.47927/1‑150 (MQ=60)

                                                                 ACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCT                                                                              >  SRR19973070.3210646/1‑150 (MQ=60)

                                                                 ACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTAAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCT                                                                              >  SRR19973070.807732/1‑150 (MQ=60)

                                                                    CGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGC                                                                           <  SRR19973070.2202677/150‑1 (MQ=60)

                                                                    CGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGC                                                                           <  SRR19973070.25333/150‑1 (MQ=60)

                                                                      GATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAA                                                                         >  SRR19973070.102502/1‑150 (MQ=60)

                                                                                AAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGG                                                               <  SRR19973070.2552203/150‑1 (MQ=60)

                                                                                AAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGG                                                               >  SRR19973070.480857/1‑150 (MQ=60)

                                                                                     TGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGAT                                                          <  SRR19973070.1232135/150‑1 (MQ=60)

                                                                                     TGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGAT                                                          <  SRR19973070.1232103/150‑1 (MQ=60)

                                                                                      GATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATT                                                         <  SRR19973070.1193679/150‑1 (MQ=60)

                                                                                        TTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTAT                                                       <  SRR19973070.1447115/150‑1 (MQ=60)

                                                                                         TGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATG                                                      <  SRR19973070.2632164/150‑1 (MQ=60)

                                                                                           CGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAG                                                    <  SRR19973070.808249/150‑1 (MQ=60)

                                                                                           CGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAG                                                    <  SRR19973070.1657947/150‑1 (MQ=60)

                                                                                              TACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGGTGTGGGCGGCGTTGGTGGGATATGTTTTGTTTGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGTTTATGATTCC                                                 >  SRR19973070.3194664/1‑150 (MQ=60)

                                                                                                CGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCAACTCTATTCAATACCGATTCTGGTGTGGGCGGCGGTGGTGGGCTATGGTTTGTTAGTTATACTCCCGGCCCACGGGGGTCTGGCCAATAAAAAAGGCGGGTTATGAGTGCGC                                               >  SRR19973070.1792596/1‑150 (MQ=60)

                                                                                                        GAACGATCGCCGCATCTGGTGGCGTTTCCCTCTCTATTCAATACCTATGCGGTTGTGGGCGGCGGTGGTGGGCTATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAATGCGGCTTATGAGTCCGCCGTTTGCT                                       >  SRR19973070.2052900/1‑150 (MQ=60)

                                                                                                        GAACGATCGCCGCATCTGGTGGCGTTTCCATCTCGATTCAATACCTATGCGGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCCACTAGGGTCTGGCAAATAAAAAAGGCGGATTATTATTCCGCCGTTTTCT                                       >  SRR19973070.2278398/1‑150 (MQ=60)

                                                                                                        GAACGATCGCCGCATCTGGTGGCGTGTCCATCTCTATTAAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGCTATGTTTTTTTATTTATACTCCCGGCCAACTAGGGTCTGGCCAATAAAAAAGGCGGATTATTATTCCGCCTTTTTCT                                       >  SRR19973070.2278410/1‑150 (MQ=60)

                                                                                                         AACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTT                                      <  SRR19973070.1252397/150‑1 (MQ=60)

                                                                                                             ATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAG                                  <  SRR19973070.2701450/150‑1 (MQ=60)

                                                                                                                 CCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTT                              <  SRR19973070.1437730/150‑1 (MQ=60)

                                                                                                                 CCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTT                              >  SRR19973070.1821657/1‑150 (MQ=60)

                                                                                                                    CATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAA                           <  SRR19973070.1457900/150‑1 (MQ=60)

                                                                                                                    CATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAAAAAAAAATGCGGATTATTAGTCCGCCTTTTGCTTTAAGTTTTTAA                           >  SRR19973070.140562/1‑150 (MQ=60)

                                                                                                                     ATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAG                          <  SRR19973070.3068813/150‑1 (MQ=60)

                                                                                                                       CTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTGGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGAT                        <  SRR19973070.2062000/150‑1 (MQ=60)

                                                                                                                       CTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGAT                        <  SRR19973070.3261098/150‑1 (MQ=60)

                                                                                                                        TGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATT                       <  SRR19973070.2751377/150‑1 (MQ=60)

                                                                                                                           TGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAAT                    <  SRR19973070.2106689/150‑1 (MQ=60)

                                                                                                                            GGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATT                   <  SRR19973070.2490273/150‑1 (MQ=60)

                                                                                                                            GGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATT                   <  SRR19973070.2489761/150‑1 (MQ=60)

                                                                                                                              CGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCA                 >  SRR19973070.3299913/1‑150 (MQ=60)

                                                                                                                                 TTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGTAGATTAATTCAGAC              >  SRR19973070.2175737/1‑150 (MQ=60)

                                                                                                                                      TCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACC         >  SRR19973070.1647205/1‑150 (MQ=60)

                                                                                                                                      TCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACC         >  SRR19973070.2903020/1‑150 (MQ=60)

                                                                                                                                           ATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCAT    <  SRR19973070.965608/150‑1 (MQ=60)

                                                                                                                                             TCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCC  <  SRR19973070.470768/150‑1 (MQ=60)

CTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCC  >  CP009273/850012‑850302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`