Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 850,161 G→T 100% P353P (CCG→CCT)  ybiR → putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273850,1610GT98.8% 553.2 / ‑1.5 183P353P (CCG→CCT) ybiRputative transporter
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (75/103);  total (78/105)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 183 total aligned reads displayed.

CTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCG  >  CP009273/850017‑850304
                                                                                                                                                |                                                                                                                                               
    gtgCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCtgt                                                                                                                                          <  1:2128640/148‑1 (MQ=255)
     tgCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCtgtt                                                                                                                                         <  1:3147353/148‑1 (MQ=255)
       ccgccgTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCtgttgt                                                                                                                                       <  1:2382723/148‑1 (MQ=255)
               tttATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGgcggcg                                                                                                                               >  2:477902/1‑148 (MQ=255)
                ttattaCTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGt                                                                                                                              <  2:3535334/148‑1 (MQ=255)
                  attaCTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTTCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTtg                                                                                                                            >  2:3443936/1‑148 (MQ=255)
                   ttaCTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTtgg                                                                                                                           <  1:1925979/148‑1 (MQ=255)
                    taCTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCGGTTGTGGGGGGCGTtggt                                                                                                                          >  1:3216253/1‑148 (MQ=255)
                       tGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTggg                                                                                                                       >  1:856212/1‑148 (MQ=255)
                       tGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTggg                                                                                                                       >  1:1022054/1‑148 (MQ=255)
                       tGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTggg                                                                                                                       >  2:2434308/1‑148 (MQ=255)
                       tGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTggg                                                                                                                       >  2:14421/1‑148 (MQ=255)
                         gTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGat                                                                                                                     <  2:2283320/148‑1 (MQ=255)
                         gTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATGGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGat                                                                                                                     <  2:1331244/148‑1 (MQ=255)
                           aTGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGatat                                                                                                                   >  1:2614434/1‑148 (MQ=255)
                           aTGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCAACTCGATTAAATACCGATGCGGTTGTGGGCGGCGGGGGTGGGgtat                                                                                                                   >  1:659099/1‑148 (MQ=255)
                           aTGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTGTCCATCTCTATTCAATAACTATGCTGTTGTGGGCGGCGTTGGTGGGttat                                                                                                                   >  1:659139/1‑148 (MQ=255)
                             gggCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGt                                                                                                                 <  2:2448109/148‑1 (MQ=255)
                                  gTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTgt                                                                                                            <  2:1484599/148‑1 (MQ=255)
                                  gTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTgt                                                                                                            <  1:3441209/148‑1 (MQ=255)
                                  gTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTgt                                                                                                            <  2:1019709/148‑1 (MQ=255)
                                   tAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTgtt                                                                                                           >  1:2141042/1‑148 (MQ=255)
                                    aaaCGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTgtta                                                                                                          >  2:3569600/1‑148 (MQ=255)
                                      aCGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTgttagt                                                                                                        <  2:1395905/148‑1 (MQ=255)
                                         tAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTtat                                                                                                     <  1:1315311/148‑1 (MQ=255)
                                         tAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTtat                                                                                                     <  2:1854632/148‑1 (MQ=255)
                                          aGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTtata                                                                                                    <  1:1432113/148‑1 (MQ=255)
                                          aGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTtata                                                                                                    <  1:103506/148‑1 (MQ=255)
                                            gTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACt                                                                                                  <  1:2239373/148‑1 (MQ=255)
                                            gTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACt                                                                                                  <  2:1218414/148‑1 (MQ=255)
                                             tGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGCTATGTTTTGTTAGTTATACTc                                                                                                 >  2:1729708/1‑148 (MQ=255)
                                             tGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTc                                                                                                 >  2:775115/1‑148 (MQ=255)
                                                 tttGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCgg                                                                                             >  2:3103159/1‑148 (MQ=255)
                                                  ttGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGCTATGTTTTGTTAGTTATACTCCCGGc                                                                                            >  2:2701764/1‑148 (MQ=255)
                                                  ttGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGc                                                                                            >  2:1267642/1‑148 (MQ=255)
                                                  ttGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGc                                                                                            >  2:442409/1‑148 (MQ=255)
                                                     ggTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCaa                                                                                         <  1:2895588/148‑1 (MQ=255)
                                                     ggTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCaa                                                                                         <  1:2895609/148‑1 (MQ=255)
                                                      gTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAAc                                                                                        <  1:1632370/148‑1 (MQ=255)
                                                           taCCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAgag                                                                                   >  2:2315364/1‑146 (MQ=255)
                                                           taCCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACGAggg                                                                                   >  2:1831332/1‑148 (MQ=255)
                                                            aCCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGt                                                                                  <  1:2451942/148‑1 (MQ=255)
                                                              ccGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCt                                                                                >  1:1542468/1‑148 (MQ=255)
                                                              ccGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGGTGGGGGCGGCGTTGGTGGGCTCTGTTTTGTTAGGTATACTCCCGGCCAACTAGGGTCt                                                                                >  2:346168/1‑148 (MQ=255)
                                                                 gATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCCTCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGc                                                                             >  1:1837879/1‑148 (MQ=255)
                                                                 gATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGc                                                                             >  2:2860663/1‑148 (MQ=255)
                                                                  aTCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCa                                                                            >  2:585862/1‑148 (MQ=255)
                                                                        gtcAAATTTGATTGCGCTACGTATGGAGAACGATCGCCTCATCTGTTTGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATaaa                                                                      <  2:2091611/146‑1 (MQ=255)
                                                                         gCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATaaaa                                                                     <  2:151982/148‑1 (MQ=255)
                                                                         gCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATaaaa                                                                     <  2:151819/148‑1 (MQ=255)
                                                                          cAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATaaaaa                                                                    <  1:175182/148‑1 (MQ=255)
                                                                          cAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATaaaaa                                                                    <  2:1350084/148‑1 (MQ=255)
                                                                              tttGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGc                                                                >  1:965996/1‑148 (MQ=255)
                                                                              tttGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGc                                                                >  1:966013/1‑148 (MQ=255)
                                                                               ttGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCg                                                               >  2:1196189/1‑148 (MQ=255)
                                                                                tGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCgg                                                              <  2:3359157/148‑1 (MQ=255)
                                                                                    tGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTa                                                          >  1:941872/1‑148 (MQ=255)
                                                                                       gcTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGa                                                       <  1:3244053/148‑1 (MQ=255)
                                                                                       gcTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGa                                                       <  2:274440/148‑1 (MQ=255)
                                                                                       gcTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGa                                                       <  1:1344890/148‑1 (MQ=255)
                                                                                       gcTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGa                                                       <  1:781144/148‑1 (MQ=255)
                                                                                         tACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGt                                                     <  1:2715037/148‑1 (MQ=255)
                                                                                         tACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGt                                                     <  2:3699467/148‑1 (MQ=255)
                                                                                              aTGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGcc                                                <  2:3187224/148‑1 (MQ=255)
                                                                                              aTGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGcc                                                <  2:571029/148‑1 (MQ=255)
                                                                                               tGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCt                                               <  1:3327055/148‑1 (MQ=255)
                                                                                                ggCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCtt                                              <  1:477902/148‑1 (MQ=255)
                                                                                                ggCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCtt                                              <  1:3346141/148‑1 (MQ=255)
                                                                                                ggCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCtt                                              <  1:3336007/148‑1 (MQ=255)
                                                                                                ggCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCtt                                              <  1:1609754/148‑1 (MQ=255)
                                                                                                    aaCGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGc                                          <  1:3621129/148‑1 (MQ=255)
                                                                                                     aCGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGGTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCt                                         >  2:2641180/1‑148 (MQ=255)
                                                                                                     aCGATCGCCGCATCTGGTGGCGTTTACATCTCTATTCAATACCTATGCTGGTGTGGGCGGCGTGGGTGGGATATGTTTTTTTAGTTATACTCCCGGCCAACGAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCt                                         >  1:2236617/1‑148 (MQ=255)
                                                                                                       gATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCttt                                       <  1:1389836/148‑1 (MQ=255)
                                                                                                          cgccgcATCTGGTGGCGTTTCCATCTCTATTCAATACCTATTCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATAATCCCCGCCAACTAGGGTCTGGCAAATAAAAAAGTCGGATTATGATTCCGCCTTTTGCTTTAAg                                    >  1:2215236/1‑148 (MQ=255)
                                                                                                           gccgcATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGt                                   <  1:1474676/148‑1 (MQ=255)
                                                                                                                aTCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGa                              <  1:1411320/148‑1 (MQ=255)
                                                                                                                 tCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATAATCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGaa                             >  2:647816/1‑148 (MQ=255)
                                                                                                                  cTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAg                            >  2:3464098/1‑148 (MQ=255)
                                                                                                                  cTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAg                            <  1:2956764/148‑1 (MQ=255)
                                                                                                                   tggtggCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGa                           <  2:1513821/148‑1 (MQ=255)
                                                                                                                    ggtggCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGAt                          <  2:1942664/148‑1 (MQ=255)
                                                                                                                      tggCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTa                        <  1:2008154/148‑1 (MQ=255)
                                                                                                                        gCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAAt                      <  1:610250/148‑1 (MQ=255)
                                                                                                                         cGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAAtt                     <  2:189632/148‑1 (MQ=255)
                                                                                                                         cGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAAtt                     <  1:668692/148‑1 (MQ=255)
                                                                                                                         cGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAAtt                     <  1:2347004/148‑1 (MQ=255)
                                                                                                                         cGTTTCCATCTCTATTCAATACCTATGCTGGTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAAtt                     <  1:2099423/148‑1 (MQ=255)
                                                                                                                           tttCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCa                   >  1:3134313/1‑148 (MQ=255)
                                                                                                                            ttCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAg                  <  2:1119460/148‑1 (MQ=255)
                                                                                                                               cATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACg               >  1:1164205/1‑148 (MQ=255)
                                                                                                                                aTCTCTAGTCAATACCTATGCTGGTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGa              <  2:3106286/148‑1 (MQ=255)
                                                                                                                                 tctcTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGaa             <  2:3236657/148‑1 (MQ=255)
                                                                                                                                  ctctATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAAc            >  1:872482/1‑148 (MQ=255)
                                                                                                                                  ctctATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAAc            >  1:3685944/1‑148 (MQ=255)
                                                                                                                                  ctctATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAAc            >  1:3684657/1‑148 (MQ=255)
                                                                                                                                    ctATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCg          >  1:1646297/1‑148 (MQ=255)
                                                                                                                                      aTTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGc        >  1:1785821/1‑148 (MQ=255)
                                                                                                                                       ttCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCa       >  2:3665114/1‑148 (MQ=255)
                                                                                                                                            tACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGTCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCg  <  2:1660726/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
CTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCG  >  CP009273/850017‑850304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGC  >  CP009273/850012‑850298
                                                                                                                                                     |                                                                                                                                         
CTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCT                                                                                                                                           >  SRR19973069.153163/1‑150 (MQ=60)
CTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCT                                                                                                                                           <  SRR19973069.1652615/150‑1 (MQ=60)
CTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCA                                                                                                                                           >  SRR19973069.153287/1‑150 (MQ=60)
CTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCA                                                                                                                                           >  SRR19973069.153327/1‑150 (MQ=60)
     CTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCT                                                                                                                                      <  SRR19973069.1468229/150‑1 (MQ=60)
         GTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTG                                                                                                                                  <  SRR19973069.2143284/150‑1 (MQ=60)
         GTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTG                                                                                                                                  >  SRR19973069.872743/1‑150 (MQ=60)
          TGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGT                                                                                                                                 <  SRR19973069.3167934/150‑1 (MQ=60)
          TGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGT                                                                                                                                 <  SRR19973069.3167936/150‑1 (MQ=60)
          TGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGT                                                                                                                                 <  SRR19973069.2144861/150‑1 (MQ=60)
            CCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGG                                                                                                                               <  SRR19973069.2398881/150‑1 (MQ=60)
            CCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGG                                                                                                                               <  SRR19973069.2398836/150‑1 (MQ=60)
                CGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGG                                                                                                                           <  SRR19973069.2610893/150‑1 (MQ=60)
                     TTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTG                                                                                                                      <  SRR19973069.3773627/150‑1 (MQ=60)
                       ATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCGGTTGTGGGGGGCGTTGGT                                                                                                                    >  SRR19973069.3237244/1‑150 (MQ=60)
                        TTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTG                                                                                                                   <  SRR19973069.1939057/150‑1 (MQ=60)
                        TTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTG                                                                                                                   <  SRR19973069.1939215/150‑1 (MQ=60)
                          ACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGG                                                                                                                 >  SRR19973069.1029126/1‑150 (MQ=60)
                          ACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGG                                                                                                                 >  SRR19973069.63248/1‑150 (MQ=60)
                          ACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGG                                                                                                                 >  SRR19973069.862208/1‑150 (MQ=60)
                             GGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATA                                                                                                              <  SRR19973069.1286619/150‑1 (MQ=60)
                              GTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTGCATCTCTATTCAATACCTATGCGGTTGTGGGCGGGGTTGGTGGGATTT                                                                                                             >  SRR19973069.663662/1‑150 (MQ=60)
                              GTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATAT                                                                                                             >  SRR19973069.2631911/1‑150 (MQ=60)
                              GTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTAAATACCAATGCGGTTGTGGGCGGCGTTGGTGGGAGAT                                                                                                             >  SRR19973069.663565/1‑150 (MQ=60)
                              GTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCAACTCGATTAAATACCGATGCGGTTGTGGGCGGCGGGGGTGGGGTAT                                                                                                             >  SRR19973069.663541/1‑150 (MQ=60)
                              GTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTGTCCATCTCTATTCAATAACTATGCTGTTGTGGGCGGCGTTGGTGGGTTAT                                                                                                             >  SRR19973069.663582/1‑150 (MQ=60)
                                    GCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTG                                                                                                       <  SRR19973069.3170447/150‑1 (MQ=60)
                                      GGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTT                                                                                                     >  SRR19973069.2155746/1‑150 (MQ=60)
                                       GTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTA                                                                                                    <  SRR19973069.3463546/150‑1 (MQ=60)
                                       GTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTA                                                                                                    <  SRR19973069.3463539/150‑1 (MQ=60)
                                          AACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGCTATGTTTTGTTAGTT                                                                                                 >  SRR19973069.1956046/1‑150 (MQ=60)
                                          AACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTT                                                                                                 <  SRR19973069.364846/150‑1 (MQ=60)
                                            CGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTAT                                                                                               <  SRR19973069.996225/150‑1 (MQ=60)
                                              TAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATTCTGGTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATAA                                                                                             >  SRR19973069.1359430/1‑150 (MQ=60)
                                              TAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATAC                                                                                             <  SRR19973069.1324337/150‑1 (MQ=60)
                                              TAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATAC                                                                                             <  SRR19973069.1324324/150‑1 (MQ=60)
                                              TAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATAC                                                                                             <  SRR19973069.3239486/150‑1 (MQ=60)
                                              TAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATAC                                                                                             <  SRR19973069.221670/150‑1 (MQ=60)
                                               AGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACT                                                                                            <  SRR19973069.3356760/150‑1 (MQ=60)
                                               AGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACT                                                                                            <  SRR19973069.104535/150‑1 (MQ=60)
                                               AGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACT                                                                                            <  SRR19973069.1442110/150‑1 (MQ=60)
                                                GGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTC                                                                                           <  SRR19973069.1433143/150‑1 (MQ=60)
                                                 GTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCC                                                                                          <  SRR19973069.2254472/150‑1 (MQ=60)
                                                          GGTTATTACCCGGATCGCTGGCACATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACT                                                                                 <  SRR19973069.2914685/150‑1 (MQ=60)
                                                          GGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACT                                                                                 <  SRR19973069.2914714/150‑1 (MQ=60)
                                                          GGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACT                                                                                 <  SRR19973069.2914712/150‑1 (MQ=60)
                                                          GGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACT                                                                                 <  SRR19973069.2914691/150‑1 (MQ=60)
                                                           GTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTA                                                                                <  SRR19973069.1643532/150‑1 (MQ=60)
                                                           GTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTA                                                                                >  SRR19973069.2642062/1‑150 (MQ=60)
                                                                 ACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCT                                                                          >  SRR19973069.1553068/1‑150 (MQ=60)
                                                                 ACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCT                                                                          >  SRR19973069.1553038/1‑150 (MQ=60)
                                                                 ACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCT                                                                          <  SRR19973069.2468434/150‑1 (MQ=60)
                                                                  CCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTAATCAATAACAATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACAAGGGTCTG                                                                         >  SRR19973069.1127689/1‑150 (MQ=60)
                                                                    CGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCCTCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGC                                                                       >  SRR19973069.1850738/1‑150 (MQ=60)
                                                                    CGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGC                                                                       >  SRR19973069.1850723/1‑150 (MQ=60)
                                                                     GGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCA                                                                      >  SRR19973069.2246765/1‑150 (MQ=60)
                                                                               CAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAG                                                            <  SRR19973069.176663/150‑1 (MQ=60)
                                                                                 AATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGC                                                          >  SRR19973069.972451/1‑150 (MQ=60)
                                                                                 AATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGC                                                          >  SRR19973069.972126/1‑150 (MQ=60)
                                                                                 AATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGC                                                          >  SRR19973069.972434/1‑150 (MQ=60)
                                                                                  ATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCG                                                         >  SRR19973069.919311/1‑150 (MQ=60)
                                                                                       ATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTA                                                    >  SRR19973069.948229/1‑150 (MQ=60)
                                                                                         TGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATG                                                  <  SRR19973069.1770401/150‑1 (MQ=60)
                                                                                            GCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGT                                               <  SRR19973069.787308/150‑1 (MQ=60)
                                                                                            GCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGT                                               <  SRR19973069.786682/150‑1 (MQ=60)
                                                                                            GCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGT                                               <  SRR19973069.1354197/150‑1 (MQ=60)
                                                                                            GCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGT                                               <  SRR19973069.3265286/150‑1 (MQ=60)
                                                                                              TACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCC                                             <  SRR19973069.2733234/150‑1 (MQ=60)
                                                                                                    TGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTT                                       <  SRR19973069.3348860/150‑1 (MQ=60)
                                                                                                     GGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCACTACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTT                                      <  SRR19973069.3527382/150‑1 (MQ=60)
                                                                                                     GGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTT                                      <  SRR19973069.481368/150‑1 (MQ=60)
                                                                                                     GGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTT                                      <  SRR19973069.3357854/150‑1 (MQ=60)
                                                                                                     GGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTT                                      <  SRR19973069.1620573/150‑1 (MQ=60)
                                                                                                     GGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTT                                      <  SRR19973069.3368032/150‑1 (MQ=60)
                                                                                                     GGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTT                                      <  SRR19973069.3368040/150‑1 (MQ=60)
                                                                                                        GAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACGAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCT                                   >  SRR19973069.2251713/1‑150 (MQ=60)
                                                                                                        GAACGATCGCCGCATCTGGTGGCGTTTACATCTCTATTCAATACCTATGCTGGTGTGGGCGGCGTGGGTGGGATATGTTTTTTTAGTTATACTCCCGGCCAACGAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCT                                   >  SRR19973069.2251706/1‑150 (MQ=60)
                                                                                                         AACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTT                                  <  SRR19973069.3644790/150‑1 (MQ=60)
                                                                                                            GATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAA                               <  SRR19973069.1399530/150‑1 (MQ=60)
                                                                                                             ATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATTCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATAATCCCCGCCAACTAGGGTCTGGCAAATAAAAAAGTCGGATTATGATTCCGCCTTTTGCTTTAAG                              >  SRR19973069.2230236/1‑150 (MQ=60)
                                                                                                                GCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTT                           <  SRR19973069.1484949/150‑1 (MQ=60)
                                                                                                                     ATCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAG                      <  SRR19973069.1421159/150‑1 (MQ=60)
                                                                                                                     ATCTGGTGGCGTTTCCATCTCTATTCAAGACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAG                      <  SRR19973069.1747853/150‑1 (MQ=60)
                                                                                                                       CTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGAT                    <  SRR19973069.2976384/150‑1 (MQ=60)
                                                                                                                       CTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGAT                    <  SRR19973069.1637949/150‑1 (MQ=60)
                                                                                                                        TGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATT                   <  SRR19973069.1487034/150‑1 (MQ=60)
                                                                                                                         GGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTA                  <  SRR19973069.61245/150‑1 (MQ=60)
                                                                                                                           TGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAAT                <  SRR19973069.2022128/150‑1 (MQ=60)
                                                                                                                             GCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTC              <  SRR19973069.614335/150‑1 (MQ=60)
                                                                                                                              CGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCA             >  SRR19973069.3154860/1‑150 (MQ=60)
                                                                                                                              CGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCA             <  SRR19973069.673263/150‑1 (MQ=60)
                                                                                                                              CGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCA             <  SRR19973069.2114237/150‑1 (MQ=60)
                                                                                                                              CGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCA             <  SRR19973069.2362967/150‑1 (MQ=60)
                                                                                                                              CGTTTCCATCTCTATTCAATACCTATGCTGGTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCA             <  SRR19973069.2113922/150‑1 (MQ=60)
                                                                                                                                  TCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACG         >  SRR19973069.1172294/1‑150 (MQ=60)
                                                                                                                                  TCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACG         <  SRR19973069.30890/150‑1 (MQ=60)
                                                                                                                                     ATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAAC      >  SRR19973069.878549/1‑150 (MQ=60)
                                                                                                                                     ATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAAC      >  SRR19973069.3710051/1‑150 (MQ=60)
                                                                                                                                     ATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAAC      >  SRR19973069.3708756/1‑150 (MQ=60)
                                                                                                                                       CTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACTAACCG    >  SRR19973069.2430009/1‑150 (MQ=60)
                                                                                                                                       CTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCG    >  SRR19973069.1657646/1‑150 (MQ=60)
                                                                                                                                         CTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGC  >  SRR19973069.1798399/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                         
CTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGC  >  CP009273/850012‑850298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑