Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F309 I0 R1	1697	197.2	6627756	95.7%	6342762	147.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,284,857	G→T	36.3%	D53Y (GAT→TAT)	rssA →	putative patatin‑like family phospholipase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,284,857	0	G	T	36.3%	199.6 / 113.6	134	D53Y (GAT→TAT)	rssA	putative patatin‑like family phospholipase
Reads supporting (aligned to +/- strand): ref base G (43/42); new base T (22/27); total (65/69)
Fisher's exact test for biased strand distribution p-value = 5.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 154 total aligned reads displayed.

TAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCCGGAAACAGAGA  >  CP009273/1284711‑1285004

tAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGcga                                                                                                                                                    <  1:766248/148‑1 (MQ=255)

 aaaaaTAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGcgat                                                                                                                                                   <  2:178961/148‑1 (MQ=255)

  aaaaTAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGctatc                                                                                                                                                  >  2:1931602/1‑148 (MQ=255)

    aaTAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGcgatcga                                                                                                                                                >  2:80984/1‑148 (MQ=255)

    aaTAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGcgatcga                                                                                                                                                >  2:2392723/1‑148 (MQ=255)

    aaTAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGcgatcga                                                                                                                                                <  1:474257/148‑1 (MQ=255)

     aTAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAATTGGGTATTGAAATTGATATCGTTGCAGGATGTTAAATTGGTTCGCTGGTGGGCGCTGCCTATGCAAGcgatcgat                                                                                                                                               >  2:106801/1‑148 (MQ=255)

     aTAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGctatcgat                                                                                                                                               <  2:3167699/148‑1 (MQ=255)

     aTAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGctatcgat                                                                                                                                               <  2:3167802/148‑1 (MQ=255)

     aTAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGctatcgat                                                                                                                                               <  2:1205341/148‑1 (MQ=255)

     aTAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGcgctcgat                                                                                                                                               >  2:2966943/1‑148 (MQ=255)

     aTAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGcgatcgat                                                                                                                                               >  2:2510565/1‑148 (MQ=255)

     aTAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGcgatcgat                                                                                                                                               <  2:279051/148‑1 (MQ=255)

     aTAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGcgatcgat                                                                                                                                               <  1:372054/148‑1 (MQ=255)

     aTAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGcgatcgat                                                                                                                                               >  2:106796/1‑148 (MQ=255)

      tAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGAtt                                                                                                                                              <  1:436204/148‑1 (MQ=255)

         ggCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATc                                                                                                                                           >  2:721297/1‑148 (MQ=255)

             ggCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTgcg                                                                                                                                       <  2:184256/148‑1 (MQ=255)

                  tGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGa                                                                                                                                  >  1:881897/1‑148 (MQ=255)

                       tCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGAtt                                                                                                                             <  1:2149728/148‑1 (MQ=255)

                          ggCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTggg                                                                                                                          <  2:2887704/148‑1 (MQ=255)

                            cgcgGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTg                                                                                                                        >  1:685642/1‑148 (MQ=255)

                                gcgAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACct                                                                                                                    <  1:1895234/148‑1 (MQ=255)

                                 cgAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACctc                                                                                                                   <  2:67023/148‑1 (MQ=255)

                                     agGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTc                                                                                                               >  1:1235113/1‑148 (MQ=255)

                                        ttGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGt                                                                                                            <  1:3115182/148‑1 (MQ=255)

                                        ttGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATGGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGt                                                                                                            <  1:3115185/148‑1 (MQ=255)

                                         tGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGtt                                                                                                           >  2:1393244/1‑148 (MQ=255)

                                         tGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGtt                                                                                                           <  1:3267088/148‑1 (MQ=255)

                                         tGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGtt                                                                                                           >  2:1393066/1‑148 (MQ=255)

                                         tGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGtt                                                                                                           >  2:1398811/1‑148 (MQ=255)

                                          ggTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTa                                                                                                          <  2:352379/148‑1 (MQ=255)

                                          ggTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTa                                                                                                          >  1:2760573/1‑148 (MQ=255)

                                           gTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTAt                                                                                                         <  1:1844410/148‑1 (MQ=255)

                                              gCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTgg                                                                                                      <  2:498522/148‑1 (MQ=255)

                                               cATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTggg                                                                                                     <  2:3001155/148‑1 (MQ=255)

                                               cATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTggg                                                                                                     <  1:3310342/148‑1 (MQ=255)

                                                 tatTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGAt                                                                                                   >  1:2839578/1‑148 (MQ=255)

                                                 tatTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGAt                                                                                                   >  1:1191297/1‑148 (MQ=255)

                                                  atTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATg                                                                                                  >  1:2993989/1‑148 (MQ=255)

                                                  atTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATg                                                                                                  <  2:3150022/148‑1 (MQ=255)

                                                   ttGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGt                                                                                                 <  2:532526/148‑1 (MQ=255)

                                                    tGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGtt                                                                                                >  2:2160995/1‑148 (MQ=255)

                                                    tGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTATTTCAGTTATTGGGATGtt                                                                                                <  1:1977128/148‑1 (MQ=255)

                                                    tGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTATTTCAGTTATTGGGATGtt                                                                                                <  1:1976758/148‑1 (MQ=255)

                                                     ggCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGttt                                                                                               <  1:758780/148‑1 (MQ=255)

                                                      gCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGtttt                                                                                              >  2:2449168/1‑148 (MQ=255)

                                                          tattaATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGc                                                                                          <  2:1973133/148‑1 (MQ=255)

                                                           attaATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGGTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGcc                                                                                         <  2:3283767/148‑1 (MQ=255)

                                                             taATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTg                                                                                       <  2:209498/148‑1 (MQ=255)

                                                             taATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTg                                                                                       >  1:1900602/1‑148 (MQ=255)

                                                             taATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTg                                                                                       >  1:764420/1‑148 (MQ=255)

                                                              aaTGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGa                                                                                      <  1:1917745/148‑1 (MQ=255)

                                                               aTGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGAt                                                                                     >  1:2050/1‑148 (MQ=255)

                                                                   gcTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGAt                                                                                 <  1:680950/148‑1 (MQ=255)

                                                                     tAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGAtct                                                                               <  2:2760573/148‑1 (MQ=255)

                                                                       aaaaaaGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGAtctct                                                                             <  1:93651/148‑1 (MQ=255)

                                                                        aaaaaGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGAtctctc                                                                            <  1:1931602/148‑1 (MQ=255)

                                                                        aaaaaGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGAtctctc                                                                            <  1:1691425/148‑1 (MQ=255)

                                                                        aaaaaGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGAtctctc                                                                            <  2:1564315/148‑1 (MQ=255)

                                                                        aaaaaGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGAtctctc                                                                            <  1:1692288/148‑1 (MQ=255)

                                                                         aaaaGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTcc                                                                           <  2:869355/148‑1 (MQ=255)

                                                                         aaaaGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTcc                                                                           <  2:3125634/148‑1 (MQ=255)

                                                                          aaaGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCt                                                                          >  1:402652/1‑148 (MQ=255)

                                                                            aGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTgg                                                                        >  1:1233514/1‑148 (MQ=255)

                                                                              tGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGAGTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCa                                                                      >  2:2381460/1‑148 (MQ=255)

                                                                              tGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCa                                                                      >  2:3300084/1‑148 (MQ=255)

                                                                                ggTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAgc                                                                    >  1:2085699/1‑148 (MQ=255)

                                                                                        aaTTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTggg                                                            >  1:3143876/1‑148 (MQ=255)

                                                                                          ttGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGtt                                                          >  1:695161/1‑148 (MQ=255)

                                                                                           tGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTa                                                         <  1:2204071/148‑1 (MQ=255)

                                                                                              tatCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTGTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTg                                                      >  2:1076523/1‑148 (MQ=255)

                                                                                              tatCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTg                                                      <  1:2510565/148‑1 (MQ=255)

                                                                                               atCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTgc                                                     >  1:12988/1‑148 (MQ=255)

                                                                                                 cGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTgcgc                                                   >  1:132953/1‑148 (MQ=255)

                                                                                                   ttGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCgcgg                                                 >  1:1656470/1‑148 (MQ=255)

                                                                                                   ttGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCgcgg                                                 <  2:640632/148‑1 (MQ=255)

                                                                                                        ggATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGc                                            >  1:1007612/1‑148 (MQ=255)

                                                                                                        ggATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGc                                            >  1:2797453/1‑148 (MQ=255)

                                                                                                        ggATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGc                                            <  1:2828506/148‑1 (MQ=255)

                                                                                                         gATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCg                                           >  2:28564/1‑148 (MQ=255)

                                                                                                         gATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCg                                           >  1:3060825/1‑148 (MQ=255)

                                                                                                            gTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCgtgt                                        <  1:1542855/148‑1 (MQ=255)

                                                                                                            gTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCgtgt                                        <  2:1682186/148‑1 (MQ=255)

                                                                                                             ttCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTc                                       >  2:1078481/1‑148 (MQ=255)

                                                                                                              tCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCt                                      >  1:2295788/1‑148 (MQ=255)

                                                                                                                 aTTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTtca                                   <  1:3176238/148‑1 (MQ=255)

                                                                                                                  ttGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTtcaa                                  <  2:1479648/148‑1 (MQ=255)

                                                                                                                    ggTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTtcaatc                                >  1:247389/1‑148 (MQ=255)

                                                                                                                               gggCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGaa                     <  1:2303211/148‑1 (MQ=255)

                                                                                                                                ggCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGaaa                    >  1:2433673/1‑148 (MQ=255)

                                                                                                                                  cgcTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAaata                  >  1:601832/1‑148 (MQ=255)

                                                                                                                                  cgcTGCCTATGCATGCGATCGAGTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAaata                  <  2:2583271/148‑1 (MQ=255)

                                                                                                                                     tGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATg               <  1:106840/148‑1 (MQ=255)

                                                                                                                                     tGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATg               <  1:106796/148‑1 (MQ=255)

                                                                                                                                          atgcatgcGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCCGGa          >  2:695741/1‑148 (MQ=255)

                                                                                                                                          atgcatgcGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCCGGa          >  2:695760/1‑148 (MQ=255)

                                                                                                                                              atgcTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCCGGAAACa      >  1:462156/1‑148 (MQ=255)

                                                                                                                                                gcGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCCGGAAACaga    >  2:2316482/1‑148 (MQ=255)

                                                                                                                                                gcGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCCGGAAACaga    >  2:2316466/1‑148 (MQ=255)

TAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCCGGAAACAGAGA  >  CP009273/1284711‑1285004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A