Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,284,857 G→T 60.1% D53Y (GAT→TAT)  rssA → putative patatin‑like family phospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,284,8570GT60.1% 47.1 / 302.5 216D53Y (GAT→TAT) rssAputative patatin‑like family phospholipase
Reads supporting (aligned to +/- strand):  ref base G (47/39);  new base T (64/66);  total (111/105)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.10e-01

Only 100 of 237 total aligned reads displayed.

ATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCCGGAAACAGAG  >  CP009273/1284710‑1285003
                                                                                                                                                   |                                                                                                                                                  
  aaaaaTAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGAGATCGGTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGcgat                                                                                                                                                  <  1:3177443/148‑1 (MQ=255)
    aaaTAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGcgatcg                                                                                                                                                >  2:1199663/1‑148 (MQ=255)
     aaTAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGctatcga                                                                                                                                               >  1:2257164/1‑148 (MQ=255)
     aaTAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGcgatcga                                                                                                                                               >  1:2917624/1‑148 (MQ=255)
     aaTAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGcgatcga                                                                                                                                               >  2:613165/1‑148 (MQ=255)
     aaTAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTAATAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGcgatcga                                                                                                                                               <  1:2648314/148‑1 (MQ=255)
      atcgggCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGctatcgat                                                                                                                                              <  2:3302429/145‑1 (MQ=255)
      aTAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGctatcgat                                                                                                                                              >  1:1340045/1‑148 (MQ=255)
        aGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTa                                                                                                                                            >  1:318786/1‑148 (MQ=255)
         gggCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTAt                                                                                                                                           <  1:397911/148‑1 (MQ=255)
         gggCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTAt                                                                                                                                           >  1:610072/1‑148 (MQ=255)
              ggCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTgcg                                                                                                                                      >  2:3279994/1‑148 (MQ=255)
                     ggATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAg                                                                                                                               <  1:1312892/148‑1 (MQ=255)
                     ggATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAg                                                                                                                               >  2:1705258/1‑148 (MQ=255)
                     ggATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGGTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAg                                                                                                                               <  1:2227464/148‑1 (MQ=255)
                       aTCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGAt                                                                                                                             <  2:1062576/148‑1 (MQ=255)
                        tCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTGTTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGAtt                                                                                                                            >  1:2978129/1‑148 (MQ=255)
                           ggCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTggg                                                                                                                         >  2:290561/1‑148 (MQ=255)
                           ggCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTggg                                                                                                                         <  1:2756272/148‑1 (MQ=255)
                                 gcgAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACct                                                                                                                   <  2:834009/148‑1 (MQ=255)
                                 gcgAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGGTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACct                                                                                                                   <  1:889133/148‑1 (MQ=255)
                                    agagGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCtt                                                                                                                >  2:306031/1‑148 (MQ=255)
                                      agGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTc                                                                                                              >  2:1940358/1‑148 (MQ=255)
                                      agGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTc                                                                                                              >  2:735906/1‑148 (MQ=255)
                                       ggTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCa                                                                                                             <  1:3031296/148‑1 (MQ=255)
                                        gTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAg                                                                                                            >  1:3054338/1‑148 (MQ=255)
                                        gTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAg                                                                                                            >  1:1146752/1‑148 (MQ=255)
                                         ttGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGt                                                                                                           <  2:1606896/148‑1 (MQ=255)
                                         ttGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGt                                                                                                           <  2:3376829/148‑1 (MQ=255)
                                          tGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGtt                                                                                                          >  2:3274438/1‑148 (MQ=255)
                                           ggTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTa                                                                                                         <  1:62026/148‑1 (MQ=255)
                                           ggTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTa                                                                                                         >  1:1689511/1‑148 (MQ=255)
                                             tCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTAtt                                                                                                       >  1:1234349/1‑148 (MQ=255)
                                                   atTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGGCCTCTTTCAGTTATTGGGATg                                                                                                 >  2:2213883/1‑148 (MQ=255)
                                                   atTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATg                                                                                                 <  1:78831/148‑1 (MQ=255)
                                                     tGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGtt                                                                                               <  1:230103/148‑1 (MQ=255)
                                                        cGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTa                                                                                            <  1:154865/148‑1 (MQ=255)
                                                             ttaATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCt                                                                                       >  2:3189175/1‑148 (MQ=255)
                                                                aTGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGAt                                                                                    <  2:606458/148‑1 (MQ=255)
                                                                 tGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATg                                                                                   <  2:552600/148‑1 (MQ=255)
                                                                 tGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATg                                                                                   >  2:239369/1‑148 (MQ=255)
                                                                     cTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGAtc                                                                               <  1:668683/148‑1 (MQ=255)
                                                                       aaaaaaaGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGAtctc                                                                             <  2:3117220/148‑1 (MQ=255)
                                                                       aaaaaaaGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGAtctc                                                                             <  2:1343364/148‑1 (MQ=255)
                                                                        aaaaaaGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGAtctct                                                                            <  2:2946253/148‑1 (MQ=255)
                                                                        aaaaaaGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGAtctct                                                                            <  1:1028043/148‑1 (MQ=255)
                                                                        aaaaaaGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGAtctct                                                                            >  1:1571080/1‑148 (MQ=255)
                                                                          aaaaGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTcc                                                                          <  1:2114675/148‑1 (MQ=255)
                                                                           aaaGTGGGTATTGAAATTGATATCGTTGCAGGATGTACAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCt                                                                         >  1:2565245/1‑148 (MQ=255)
                                                                             aGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTgg                                                                       >  2:2273341/1‑148 (MQ=255)
                                                                              gTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGa                                                                                                   >  1:36934/1‑119 (MQ=255)
                                                                              gTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGa                                                                                                   <  2:36934/119‑1 (MQ=255)
                                                                              gTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGc                                                                      >  2:910629/1‑148 (MQ=255)
                                                                               tGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCa                                                                     >  1:2955157/1‑148 (MQ=255)
                                                                               tGGGTATTGAAATTGATAACGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCATATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGAGCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCa                                                                     >  2:1091401/1‑148 (MQ=255)
                                                                                   tATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAgcgc                                                                 >  1:1827765/1‑148 (MQ=255)
                                                                                     ttGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCgg                                                               >  1:723062/1‑148 (MQ=255)
                                                                                      tGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGt                                                              <  2:896195/148‑1 (MQ=255)
                                                                                      tGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGt                                                              >  1:2188781/1‑148 (MQ=255)
                                                                                       gAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTg                                                             <  2:1245775/148‑1 (MQ=255)
                                                                                       gAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTg                                                             <  1:1738720/148‑1 (MQ=255)
                                                                                       gAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTg                                                             <  1:3500575/148‑1 (MQ=255)
                                                                                          aTTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGt                                                          >  1:458853/1‑148 (MQ=255)
                                                                                          aTTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGt                                                          <  2:256417/148‑1 (MQ=255)
                                                                                           ttGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGtt                                                         >  2:1411069/1‑148 (MQ=255)
                                                                                              atatCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACt                                                      >  1:2291755/1‑148 (MQ=255)
                                                                                                atCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTTCCTATGCATGCTATCGATTATCTTCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTgc                                                    >  1:3082388/1‑148 (MQ=255)
                                                                                                atCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTgc                                                    >  2:2965275/1‑148 (MQ=255)
                                                                                                  cGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTgcgc                                                  >  2:1614103/1‑148 (MQ=255)
                                                                                                      gCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCgcggcg                                              <  1:557013/148‑1 (MQ=255)
                                                                                                        aGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAg                                            <  2:557330/148‑1 (MQ=255)
                                                                                                          gATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCg                                          >  2:2101418/1‑148 (MQ=255)
                                                                                                           aTGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCgt                                         >  2:709595/1‑148 (MQ=255)
                                                                                                            tGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCgtg                                        >  1:3212259/1‑148 (MQ=255)
                                                                                                            tGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCgtg                                        >  1:2486436/1‑148 (MQ=255)
                                                                                                            tGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCgtg                                        >  1:3211001/1‑148 (MQ=255)
                                                                                                              ttCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTc                                      <  1:441918/148‑1 (MQ=255)
                                                                                                              ttCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTc                                      <  1:2866499/148‑1 (MQ=255)
                                                                                                                  aatGGTTCGCTGGAGGGCGCTCCCTATGCATGCTATCGATTATCTGCGCAGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGTATCTCTAATGGCAGCGCGGTTGGTTACTTCGCTGCGAGCGTGTCTtca                                  >  2:1579721/3‑148 (MQ=255)
                                                                                                                  aTTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTtca                                  >  1:2661349/1‑148 (MQ=255)
                                                                                                                   ttGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTtcaa                                 >  1:2053381/1‑148 (MQ=255)
                                                                                                                   ttGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTtcaa                                 <  2:1808676/148‑1 (MQ=255)
                                                                                                                   ttGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTtcaa                                 <  1:1926348/148‑1 (MQ=255)
                                                                                                                   ttGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTtcaa                                 <  1:1255799/148‑1 (MQ=255)
                                                                                                                   ttGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTtcaa                                 <  1:1404639/148‑1 (MQ=255)
                                                                                                                    tGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGACTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTtcaat                                >  2:2147144/1‑148 (MQ=255)
                                                                                                                    tGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTtcaat                                >  1:3294890/1‑148 (MQ=255)
                                                                                                                      gTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTtcaatca                              <  1:1097888/148‑1 (MQ=255)
                                                                                                                      gTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGTTTATCTGAGCTTGAAGTTTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGGAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTtcaatca                              <  2:2615119/148‑1 (MQ=255)
                                                                                                                         cGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATa                           <  1:1233191/148‑1 (MQ=255)
                                                                                                                                 ggCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGaaa                   <  2:2718537/148‑1 (MQ=255)
                                                                                                                                  gcgcTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAaat                  >  1:3183827/1‑148 (MQ=255)
                                                                                                                                   cgcTGCCTATGCATGCGATCGTTTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAaata                 <  2:1419667/148‑1 (MQ=255)
                                                                                                                                      tGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATg              >  2:80902/1‑148 (MQ=255)
                                                                                                                                        ccTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGcc            <  2:3430751/148‑1 (MQ=255)
                                                                                                                                          tATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCCgg          >  2:2738381/1‑148 (MQ=255)
                                                                                                                                             gcatgcGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCCGGaaa       <  2:2917624/148‑1 (MQ=255)
                                                                                                                                               atgcGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCCGGAAACa     <  2:2978129/148‑1 (MQ=255)
                                                                                                                                                  cgatcgatTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTTTTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCCGGAAACagag  <  1:2078050/148‑1 (MQ=255)
                                                                                                                                                  cgatcgatTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCCGGAAACagag  >  2:3481469/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCCGGAAACAGAG  >  CP009273/1284710‑1285003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCCGGAAACAGAGAT  >  CP009273/1284709‑1285005
                                                                                                                                                    |                                                                                                                                                    
GATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTA                                                                                                                                                     >  SRR19973066.369755/1‑150 (MQ=60)
GATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGA                                                                                                                                                     >  SRR19973066.819321/1‑150 (MQ=60)
  TAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATC                                                                                                                                                   <  SRR19973066.739524/150‑1 (MQ=60)
   AAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCG                                                                                                                                                  >  SRR19973066.1632969/1‑150 (MQ=60)
   AAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGGTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCG                                                                                                                                                  <  SRR19973066.3198594/150‑1 (MQ=60)
   AAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGAGATCGGTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCG                                                                                                                                                  <  SRR19973066.3198589/150‑1 (MQ=60)
    AAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGA                                                                                                                                                 >  SRR19973066.2272716/1‑150 (MQ=60)
    AAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGA                                                                                                                                                 >  SRR19973066.2937085/1‑150 (MQ=60)
     AAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGAT                                                                                                                                                >  SRR19973066.1349947/1‑150 (MQ=60)
     AAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGAT                                                                                                                                                >  SRR19973066.1349771/1‑150 (MQ=60)
     AAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGAT                                                                                                                                                >  SRR19973066.1349766/1‑150 (MQ=60)
      AATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATT                                                                                                                                               >  SRR19973066.2300244/1‑150 (MQ=60)
      AATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTAATAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATT                                                                                                                                               <  SRR19973066.2666099/150‑1 (MQ=60)
       ATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTA                                                                                                                                              >  SRR19973066.341536/1‑150 (MQ=60)
       ATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTA                                                                                                                                              >  SRR19973066.321176/1‑150 (MQ=60)
        TAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATTCGCTAAAAAAAGTGGGTATTGAAATTGAAATCGTTGCAGGATGGTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTAT                                                                                                                                             >  SRR19973066.614316/1‑150 (MQ=60)
        TAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGCTGTTCAATTGGTTCGCTCGTGGTCGATGCCTATGCATGCTATAGATTAT                                                                                                                                             >  SRR19973066.1875667/1‑150 (MQ=60)
        TAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTAT                                                                                                                                             >  SRR19973066.614323/1‑150 (MQ=60)
        TAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTAT                                                                                                                                             >  SRR19973066.1875793/1‑150 (MQ=60)
          GGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCT                                                                                                                                           <  SRR19973066.3454527/150‑1 (MQ=60)
          GGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCT                                                                                                                                           <  SRR19973066.400590/150‑1 (MQ=60)
          GGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCT                                                                                                                                           <  SRR19973066.401045/150‑1 (MQ=60)
            GCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGC                                                                                                                                         >  SRR19973066.1199383/1‑150 (MQ=60)
             CTGGCGCTGGGATCTGGCGCGGCGAGTGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCG                                                                                                                                        <  SRR19973066.3097057/150‑1 (MQ=60)
                  GCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGAGATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGA                                                                                                                                   <  SRR19973066.1096477/150‑1 (MQ=60)
                      GGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGAT                                                                                                                               <  SRR19973066.1322418/150‑1 (MQ=60)
                      GGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGAT                                                                                                                               <  SRR19973066.2038386/150‑1 (MQ=60)
                      GGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGAT                                                                                                                               >  SRR19973066.430113/1‑150 (MQ=60)
                      GGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGGTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGAT                                                                                                                               <  SRR19973066.2242855/150‑1 (MQ=60)
                       GATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTGTTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATT                                                                                                                              >  SRR19973066.2997807/1‑150 (MQ=60)
                         TCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGG                                                                                                                            >  SRR19973066.467304/1‑150 (MQ=60)
                         TCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGG                                                                                                                            >  SRR19973066.2271929/1‑150 (MQ=60)
                            GGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTG                                                                                                                         <  SRR19973066.2774836/150‑1 (MQ=60)
                             GCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGA                                                                                                                        <  SRR19973066.2091547/150‑1 (MQ=60)
                                  GCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGGTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCT                                                                                                                   <  SRR19973066.895440/150‑1 (MQ=60)
                                   CGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATNTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTT                                                                                                                  >  SRR19973066.1821523/1‑150 (MQ=60)
                                     AGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTC                                                                                                                <  SRR19973066.481891/150‑1 (MQ=60)
                                       AGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAG                                                                                                              >  SRR19973066.3074765/1‑150 (MQ=60)
                                       AGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAG                                                                                                              >  SRR19973066.1154935/1‑150 (MQ=60)
                                       AGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTAAG                                                                                                              >  SRR19973066.438325/1‑150 (MQ=60)
                                        GGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGT                                                                                                             <  SRR19973066.3051549/150‑1 (MQ=60)
                                        GGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGT                                                                                                             <  SRR19973066.3051566/150‑1 (MQ=60)
                                          TTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTA                                                                                                           >  SRR19973066.1701807/1‑150 (MQ=60)
                                            GGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATT                                                                                                         <  SRR19973066.62790/150‑1 (MQ=60)
                                            GGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATT                                                                                                         >  SRR19973066.1243123/1‑150 (MQ=60)
                                            GGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATT                                                                                                         >  SRR19973066.2657742/1‑150 (MQ=60)
                                             GTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTG                                                                                                        <  SRR19973066.3232732/150‑1 (MQ=60)
                                                GCATATTGGCGTTATTAATGCGCTAAAAATAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGTCGCTGGCTATGAATGCTATCGATTATCTGCGCTGGATGATTGGGTGACCTCTTTCAGTTATTGGGA                                                                                                     <  SRR19973066.2675941/150‑1 (MQ=60)
                                                 CATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGAT                                                                                                    >  SRR19973066.2806948/1‑150 (MQ=60)
                                                 CATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGAT                                                                                                    >  SRR19973066.2806823/1‑150 (MQ=60)
                                                  ATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATG                                                                                                   <  SRR19973066.67156/150‑1 (MQ=60)
                                                    ATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTTATAACGTTTCAGGATGTTCAAATTGTTCGCTGGTGGGCGCTGCCAATGCATGCTATCGATTATCTTCGCTGGAAGATTTGGTGACCTCTTTAAGTTATTGGGATGTT                                                                                                 >  SRR19973066.2123602/1‑150 (MQ=60)
                                                    ATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTT                                                                                                 <  SRR19973066.79745/150‑1 (MQ=60)
                                                     TTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTT                                                                                                >  SRR19973066.2516964/1‑150 (MQ=60)
                                                      TGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTT                                                                                               <  SRR19973066.231087/150‑1 (MQ=60)
                                                      TGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTT                                                                                               <  SRR19973066.231935/150‑1 (MQ=60)
                                                         CGTTATTAATGCTCTAAAAAAAGTGGCTATTGAACTTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACG                                                                                            <  SRR19973066.2707482/150‑1 (MQ=60)
                                                         CGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACG                                                                                            <  SRR19973066.156298/150‑1 (MQ=60)
                                                         CGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACG                                                                                            >  SRR19973066.1311229/1‑150 (MQ=60)
                                                         CGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACG                                                                                            <  SRR19973066.156303/150‑1 (MQ=60)
                                                         CGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACG                                                                                            <  SRR19973066.157869/150‑1 (MQ=60)
                                                            TATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCT                                                                                         >  SRR19973066.2422531/1‑150 (MQ=60)
                                                               TAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGAT                                                                                      >  SRR19973066.81253/1‑150 (MQ=60)
                                                                      CTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTC                                                                               <  SRR19973066.673567/150‑1 (MQ=60)
                                                                       TAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCT                                                                              >  SRR19973066.682967/1‑150 (MQ=60)
                                                                       TAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCT                                                                              >  SRR19973066.1514465/1‑150 (MQ=60)
                                                                       TAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCT                                                                              >  SRR19973066.1582405/1‑150 (MQ=60)
                                                                       TAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCT                                                                              >  SRR19973066.654583/1‑150 (MQ=60)
                                                                        AAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTC                                                                             >  SRR19973066.1251596/1‑150 (MQ=60)
                                                                         AAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCC                                                                            <  SRR19973066.1035613/150‑1 (MQ=60)
                                                                          AAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTACAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCT                                                                           >  SRR19973066.2582655/1‑150 (MQ=60)
                                                                           AAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTG                                                                          <  SRR19973066.2129291/150‑1 (MQ=60)
                                                                           AAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTG                                                                          <  SRR19973066.3300645/150‑1 (MQ=60)
                                                                             AAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGAggagatcggaagagcacacgtctgaactc                                                                        >  SRR19973066.37429/1‑121 (MQ=60)
                                                                              AGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCA                                                                       >  SRR19973066.2974617/1‑150 (MQ=60)
                                                                              AGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCA                                                                       >  SRR19973066.2974748/1‑150 (MQ=60)
                                                                                  GGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGC                                                                   >  SRR19973066.1840811/1‑150 (MQ=60)
                                                                                    TATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGG                                                                 >  SRR19973066.728422/1‑150 (MQ=60)
                                                                                     ATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGT                                                                >  SRR19973066.2203966/1‑150 (MQ=60)
                                                                                     ATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGT                                                                >  SRR19973066.2203987/1‑150 (MQ=60)
                                                                                        GAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGG                                                             <  SRR19973066.3523916/150‑1 (MQ=60)
                                                                                        GAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGG                                                             <  SRR19973066.1751269/150‑1 (MQ=60)
                                                                                         AAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGT                                                            >  SRR19973066.462371/1‑150 (MQ=60)
                                                                                          AATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTT                                                           <  SRR19973066.2205209/150‑1 (MQ=60)
                                                                                           ATTGATATCGTTGCAGGATGTTCAATTGGTCCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTA                                                          >  SRR19973066.1141334/1‑150 (MQ=60)
                                                                                             TGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACT                                                        >  SRR19973066.2307467/1‑150 (MQ=60)
                                                                                             TGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACT                                                        >  SRR19973066.2308619/1‑150 (MQ=60)
                                                                                             TGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACT                                                        <  SRR19973066.1745388/150‑1 (MQ=60)
                                                                                               ATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTTCCTATGCATGCTATCGATTATCTTCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGC                                                      >  SRR19973066.3102975/1‑150 (MQ=60)
                                                                                               ATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGC                                                      >  SRR19973066.1315486/1‑150 (MQ=60)
                                                                                                       GCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAG                                              <  SRR19973066.2962453/150‑1 (MQ=60)
                                                                                                       GCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAG                                              <  SRR19973066.561003/150‑1 (MQ=60)
                                                                                                          GGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGT                                           >  SRR19973066.3063653/1‑150 (MQ=60)
                                                                                                          GGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGT                                           >  SRR19973066.2997681/1‑150 (MQ=60)
                                                                                                           GATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTG                                          >  SRR19973066.3232282/1‑150 (MQ=60)
                                                                                                           GATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTG                                          >  SRR19973066.3233542/1‑150 (MQ=60)
                                                                                                           GATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTG                                          >  SRR19973066.2503399/1‑150 (MQ=60)
                                                                                                            ATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGT                                         >  SRR19973066.731165/1‑150 (MQ=60)
                                                                                                              GTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCT                                       <  SRR19973066.3590307/150‑1 (MQ=60)
                                                                                                               TTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTT                                      <  SRR19973066.445333/150‑1 (MQ=60)
                                                                                                               TTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTT                                      <  SRR19973066.3010116/150‑1 (MQ=60)
                                                                                                               TTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTT                                      <  SRR19973066.2885731/150‑1 (MQ=60)
                                                                                                               TTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTT                                      <  SRR19973066.2603460/150‑1 (MQ=60)
                                                                                                                 CAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCA                                    >  SRR19973066.2679183/1‑150 (MQ=60)
                                                                                                                 CAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCA                                    >  SRR19973066.292646/1‑150 (MQ=60)
                                                                                                                  AATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAA                                   >  SRR19973066.2067637/1‑150 (MQ=60)
                                                                                                                   ATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAAT                                  >  SRR19973066.3316647/1‑150 (MQ=60)
                                                                                                                    TTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATC                                 <  SRR19973066.1940047/150‑1 (MQ=60)
                                                                                                                    TTGGTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATC                                 <  SRR19973066.1264844/150‑1 (MQ=60)
                                                                                                                    TTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATC                                 <  SRR19973066.1414746/150‑1 (MQ=60)
                                                                                                                       GTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAAT                              <  SRR19973066.1105871/150‑1 (MQ=60)
                                                                                                                       GTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAAT                              <  SRR19973066.1660435/150‑1 (MQ=60)
                                                                                                                       GTTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAAT                              <  SRR19973066.3210377/150‑1 (MQ=60)
                                                                                                                       GTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATCGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAAT                              <  SRR19973066.2115889/150‑1 (MQ=60)
                                                                                                                        TTCGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATA                             <  SRR19973066.458005/150‑1 (MQ=60)
                                                                                                                          CGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACC                           <  SRR19973066.1241954/150‑1 (MQ=60)
                                                                                                                          CGCTGGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACC                           <  SRR19973066.1242514/150‑1 (MQ=60)
                                                                                                                              GGTGGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGA                       >  SRR19973066.3227750/1‑150 (MQ=60)
                                                                                                                                 GGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAAT                    >  SRR19973066.3205224/1‑150 (MQ=60)
                                                                                                                                 GGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAAT                    >  SRR19973066.3205007/1‑150 (MQ=60)
                                                                                                                                 GGGCGCTGCCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAAT                    <  SRR19973066.3199692/150‑1 (MQ=60)
                                                                                                                                    CGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAAT                 <  SRR19973066.617450/150‑1 (MQ=60)
                                                                                                                                         CCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCCGG            >  SRR19973066.1224645/1‑150 (MQ=60)
                                                                                                                                         CCTATGCATGCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCCGG            <  SRR19973066.2133331/150‑1 (MQ=60)
                                                                                                                                                 TGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCCGGAAACAGAG    <  SRR19973066.3187219/150‑1 (MQ=60)
                                                                                                                                                  GCTATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCCGGAAACAGAGA   <  SRR19973066.1280106/150‑1 (MQ=60)
                                                                                                                                                   CGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTTTTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCCGGAAACAGAGAT  <  SRR19973066.2092409/150‑1 (MQ=60)
                                                                                                                                                   CGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCCGGAAACAGAGAT  >  SRR19973066.3168368/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                    
GATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCCGGAAACAGAGAT  >  CP009273/1284709‑1285005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑