Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,412,385 G→T 100% N34K (AAC→AAA)  kilR ← Rac prophage; inhibitor of ftsZ, killing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,412,3850GT93.8% 671.7 / 31.5 237N34K (AAC→AAA) kilRRac prophage; inhibitor of ftsZ, killing protein
Reads supporting (aligned to +/- strand):  ref base G (6/9);  new base T (98/122);  total (104/133)
Fisher's exact test for biased strand distribution p-value = 7.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 239 total aligned reads displayed.

TAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTGTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGTGT  >  CP009273/1412240‑1412528
                                                                                                                                                 |                                                                                                                                               
tAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATtttt                                                                                                                                               >  1:1411487/1‑148 (MQ=255)
   gCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGc                                                                                                                                            >  2:286723/1‑148 (MQ=255)
   gCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGc                                                                                                                                            <  2:2034972/148‑1 (MQ=255)
     acaTTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCg                                                                                                                                          <  1:2861548/148‑1 (MQ=255)
      caTTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGa                                                                                                                                         >  1:3161481/1‑148 (MQ=255)
      caTTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTGTTTGCCGa                                                                                                                                         >  2:1113629/1‑148 (MQ=255)
        ttttattttCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAgg                                                                                                                                       >  1:2898546/1‑148 (MQ=255)
        ttttattttCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAgg                                                                                                                                       >  1:2781245/1‑148 (MQ=255)
        ttttattttCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAgg                                                                                                                                       >  1:712725/1‑148 (MQ=255)
         tttattttCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGACGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGc                                                                                                                                      >  1:2491987/1‑148 (MQ=255)
         tttattttCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGc                                                                                                                                      >  1:313026/1‑148 (MQ=255)
         tttattttCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGc                                                                                                                                      >  2:2123776/1‑148 (MQ=255)
          ttattttCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTGTTTGCCGAGGCa                                                                                                                                     <  1:3226143/148‑1 (MQ=255)
           tattttCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCaa                                                                                                                                    <  2:235753/148‑1 (MQ=255)
            attttCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAat                                                                                                                                   >  2:1799604/1‑148 (MQ=255)
            attttCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAat                                                                                                                                   >  1:661511/1‑148 (MQ=255)
              tttCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAatat                                                                                                                                 <  1:228172/148‑1 (MQ=255)
                 cTTAGTCATCACCATGACTCCGCCATTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATg                                                                                                                              <  2:2927201/148‑1 (MQ=255)
                  ttAGTCATCACCATGACTCCGCCTTTACAGGTAAACAATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGt                                                                                                                             <  2:2558336/148‑1 (MQ=255)
                   tAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTc                                                                                                                            >  1:2428444/1‑148 (MQ=255)
                    aGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTcc                                                                                                                           <  1:1524842/148‑1 (MQ=255)
                     gTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCg                                                                                                                          <  1:303519/148‑1 (MQ=255)
                       catcaCCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGc                                                                                                                        <  1:2165952/148‑1 (MQ=255)
                        atcaCCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGcc                                                                                                                       >  2:1713297/1‑148 (MQ=255)
                            ccATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCa                                                                                                                   >  2:3299331/1‑148 (MQ=255)
                                gACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATgaa                                                                                                               <  1:211056/148‑1 (MQ=255)
                                gACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATgaa                                                                                                               >  2:657409/1‑148 (MQ=255)
                                  cTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATgaaga                                                                                                             >  1:2388497/1‑148 (MQ=255)
                                  cTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATgaaga                                                                                                             <  1:1834323/148‑1 (MQ=255)
                                     cGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACa                                                                                                          <  2:2013637/148‑1 (MQ=255)
                                       ccTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATa                                                                                                        <  2:1523805/148‑1 (MQ=255)
                                       ccTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATa                                                                                                        <  2:1524325/148‑1 (MQ=255)
                                         tttACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAAt                                                                                                      <  2:2424971/148‑1 (MQ=255)
                                         tttACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAAt                                                                                                      <  1:1786191/148‑1 (MQ=255)
                                          ttACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGACGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATc                                                                                                     >  2:1881167/1‑148 (MQ=255)
                                           tACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCg                                                                                                    <  1:2212157/148‑1 (MQ=255)
                                               ggTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCAGGCCTTCATGAAGAACATAATCGCCAg                                                                                                >  2:1840852/1‑148 (MQ=255)
                                                  aaaCCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAg                                                                                             >  2:2264338/1‑148 (MQ=255)
                                                   aaCCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGt                                                                                            <  1:1907271/148‑1 (MQ=255)
                                                      cATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTcac                                                                                         <  2:948355/148‑1 (MQ=255)
                                                         cACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTcacaca                                                                                      >  1:1290028/1‑148 (MQ=255)
                                                          aCGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATTTTTTTGTGGTCAGTTTACTAATATATAGTTTTCGATTTTTAATATTTTTTGACGAGGCAATATATGTCCGGCCTTCATGAAGAAAATAATCGCCAGGAGTCacacac                                                                                     <  1:562046/148‑1 (MQ=255)
                                                               cGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACg                                                                                >  1:19994/1‑148 (MQ=255)
                                                                  ggAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTgg                                                                             >  2:3058445/1‑148 (MQ=255)
                                                                  ggAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTgg                                                                             >  1:1195483/1‑148 (MQ=255)
                                                                   gAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGt                                                                            >  1:2316725/1‑148 (MQ=255)
                                                                     aGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGTCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTAt                                                                          <  2:2680665/148‑1 (MQ=255)
                                                                     aGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTAt                                                                          <  1:755175/148‑1 (MQ=255)
                                                                     aGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTAt                                                                          <  1:1612677/148‑1 (MQ=255)
                                                                     aGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTGTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTAt                                                                          <  2:781502/148‑1 (MQ=255)
                                                                            aaTCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTtcatca                                                                   <  2:1054282/148‑1 (MQ=255)
                                                                             aTCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAg                                                                  >  1:588465/1‑148 (MQ=255)
                                                                              tCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGt                                                                 >  2:2166978/1‑148 (MQ=255)
                                                                              tCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGt                                                                 >  1:3019183/1‑148 (MQ=255)
                                                                                aTGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTc                                                               <  2:217838/148‑1 (MQ=255)
                                                                                      tCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGt                                                         <  2:97785/148‑1 (MQ=255)
                                                                                      tCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGt                                                         <  2:528087/148‑1 (MQ=255)
                                                                                          tAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGAtg                                                     >  1:1299138/1‑148 (MQ=255)
                                                                                          tAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGAtg                                                     >  1:1467526/1‑148 (MQ=255)
                                                                                               aatgTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCaa                                                <  2:263553/148‑1 (MQ=255)
                                                                                                atgTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAAt                                               <  2:41781/148‑1 (MQ=255)
                                                                                                atgTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAAt                                               <  2:1063050/148‑1 (MQ=255)
                                                                                                atgTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAAt                                               <  2:1469781/148‑1 (MQ=255)
                                                                                                  gTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCa                                             <  2:3076552/148‑1 (MQ=255)
                                                                                                   tttttGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCAt                                            >  1:1001266/1‑148 (MQ=255)
                                                                                                   tttttGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCAt                                            >  1:2636215/1‑148 (MQ=255)
                                                                                                   tttttGGGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCAt                                            <  2:2082396/148‑1 (MQ=255)
                                                                                                     tttGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCTAAGTGATGTGCAATCATaa                                          <  2:411481/148‑1 (MQ=255)
                                                                                                     tttGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATaa                                          <  1:105908/148‑1 (MQ=255)
                                                                                                            tCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTAtctc                                   <  2:1164706/148‑1 (MQ=255)
                                                                                                            tCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTAtctc                                   <  2:627836/148‑1 (MQ=255)
                                                                                                               ggTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTACAt                                >  2:3174134/1‑148 (MQ=255)
                                                                                                                 ttACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGTAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCAttt                              >  1:769220/1‑148 (MQ=255)
                                                                                                                 ttACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCAttt                              <  1:98420/148‑1 (MQ=255)
                                                                                                                 ttACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCAttt                              >  1:769361/1‑148 (MQ=255)
                                                                                                                   aCGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCAttttt                            <  2:1282582/148‑1 (MQ=255)
                                                                                                                   aCGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCAttttt                            <  1:2463366/148‑1 (MQ=255)
                                                                                                                      aaTATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACa                         >  2:2108763/1‑148 (MQ=255)
                                                                                                                      aaTATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACa                         >  2:726259/1‑148 (MQ=255)
                                                                                                                       atatatAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACaa                        <  2:100557/148‑1 (MQ=255)
                                                                                                                         atataGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCAtt                               >  1:1088772/1‑139 (MQ=255)
                                                                                                                         atataGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAAt                      <  1:261357/148‑1 (MQ=255)
                                                                                                                         atataGTTTTCGCTTTTTAATATT‑TTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATg                     <  1:1268750/148‑1 (MQ=255)
                                                                                                                          tataGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATg                     >  2:474952/1‑148 (MQ=255)
                                                                                                                           ataGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGa                    <  1:1169815/148‑1 (MQ=255)
                                                                                                                           ataGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGa                    <  1:2584474/148‑1 (MQ=255)
                                                                                                                            taGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGaa                   <  2:712725/148‑1 (MQ=255)
                                                                                                                              gTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACt                 <  2:3019183/148‑1 (MQ=255)
                                                                                                                               ttttCTTTTTTTAATATTTTTTTCCGAGGCAATTTATGTGCGGACTTCATTATTTAAATAATCGCCATGAGTCACACACTGACGTGGTATTTCATCAGTTCCGATGTGTTGTGCAATCATAATTATCTCCATTTTTACATATGAACtt                <  1:3057434/148‑1 (MQ=255)
                                                                                                                               ttttCGATTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACtt                <  1:2551913/148‑1 (MQ=255)
                                                                                                                                tttCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACttt               >  2:2944171/1‑148 (MQ=255)
                                                                                                                                tttCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACttt               >  2:932962/1‑148 (MQ=255)
                                                                                                                                 ttCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTttg              <  1:1881167/148‑1 (MQ=255)
                                                                                                                                  tCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTttgt             >  2:2779234/1‑148 (MQ=255)
                                                                                                                                    gCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTttgttg           <  2:661512/148‑1 (MQ=255)
                                                                                                                                     cTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGa          >  2:866253/1‑148 (MQ=255)
                                                                                                                                       ttttAATATTTTTTGCCGAGGCTATATATGTCCGGCATTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATg        <  1:2123776/148‑1 (MQ=255)
                                                                                                                                        tttAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGc       >  2:3134359/1‑148 (MQ=255)
                                                                                                                                           aaTATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGgt    >  2:1646118/1‑148 (MQ=255)
                                                                                                                                             tatTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGgtgt  >  2:3078248/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTGTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGTGT  >  CP009273/1412240‑1412528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTGTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGTGTCTG  >  CP009273/1412238‑1412531
                                                                                                                                                   |                                                                                                                                                  
GGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTT                                                                                                                                                  >  SRR19973070.1421121/1‑150 (MQ=60)
 GTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTT                                                                                                                                                 <  SRR19973070.3276106/150‑1 (MQ=60)
 GTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTT                                                                                                                                                 <  SRR19973070.1628209/150‑1 (MQ=60)
  TAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTG                                                                                                                                                >  SRR19973070.1388968/1‑150 (MQ=60)
     GCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCG                                                                                                                                             <  SRR19973070.2453462/150‑1 (MQ=60)
      CACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGA                                                                                                                                            >  SRR19973070.3181939/1‑150 (MQ=60)
       ACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAG                                                                                                                                           <  SRR19973070.2880091/150‑1 (MQ=60)
       ACATTTTATTTTATTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAG                                                                                                                                           <  SRR19973070.2880294/150‑1 (MQ=60)
        CATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGG                                                                                                                                          >  SRR19973070.718129/1‑150 (MQ=60)
        CATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGG                                                                                                                                          >  SRR19973070.717810/1‑150 (MQ=60)
        CATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGG                                                                                                                                          >  SRR19973070.2917295/1‑150 (MQ=60)
        CATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGG                                                                                                                                          >  SRR19973070.2917333/1‑150 (MQ=60)
        CATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGG                                                                                                                                          >  SRR19973070.2799120/1‑150 (MQ=60)
         ATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGACGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGC                                                                                                                                         >  SRR19973070.2508157/1‑150 (MQ=60)
         ATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGC                                                                                                                                         >  SRR19973070.315272/1‑150 (MQ=60)
         ATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGC                                                                                                                                         >  SRR19973070.1845780/1‑150 (MQ=60)
         ATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGC                                                                                                                                         >  SRR19973070.1846038/1‑150 (MQ=60)
            TTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAAT                                                                                                                                      >  SRR19973070.666229/1‑150 (MQ=60)
            TTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAAT                                                                                                                                      >  SRR19973070.666230/1‑150 (MQ=60)
            TTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTGTTTGCCGAGGCAAT                                                                                                                                      <  SRR19973070.3246985/150‑1 (MQ=60)
                TTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATAT                                                                                                                                  <  SRR19973070.229796/150‑1 (MQ=60)
                   CTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTC                                                                                                                               >  SRR19973070.2444391/1‑150 (MQ=60)
                   CTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTC                                                                                                                               >  SRR19973070.775285/1‑150 (MQ=60)
                    TTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCC                                                                                                                              <  SRR19973070.2172618/150‑1 (MQ=60)
                      AGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGG                                                                                                                            <  SRR19973070.1535550/150‑1 (MQ=60)
                      AGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGG                                                                                                                            <  SRR19973070.1536021/150‑1 (MQ=60)
                      AGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTGTTTGCCGAGGCAATATATGTCCGG                                                                                                                            >  SRR19973070.3239165/1‑150 (MQ=60)
                       GTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGC                                                                                                                           <  SRR19973070.305648/150‑1 (MQ=60)
                       GTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGC                                                                                                                           <  SRR19973070.305656/150‑1 (MQ=60)
                         CATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCT                                                                                                                         <  SRR19973070.2180327/150‑1 (MQ=60)
                          ATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTT                                                                                                                        >  SRR19973070.2949642/1‑150 (MQ=60)
                          ATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTT                                                                                                                        >  SRR19973070.1291538/1‑150 (MQ=60)
                            CACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGACGAGGCAATATATGTCCGGCCTTCA                                                                                                                      >  SRR19973070.3157223/1‑150 (MQ=60)
                                ATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAA                                                                                                                  >  SRR19973070.942384/1‑150 (MQ=60)
                                  GACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGA                                                                                                                >  SRR19973070.2404272/1‑150 (MQ=60)
                                  GACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGA                                                                                                                <  SRR19973070.212615/150‑1 (MQ=60)
                                   ACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAA                                                                                                               >  SRR19973070.3057052/1‑150 (MQ=60)
                                   ACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAA                                                                                                               >  SRR19973070.101521/1‑150 (MQ=60)
                                    CTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAAC                                                                                                              <  SRR19973070.1846932/150‑1 (MQ=60)
                                    CTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAAC                                                                                                              <  SRR19973070.1846937/150‑1 (MQ=60)
                                          CTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATC                                                                                                        <  SRR19973070.1293814/150‑1 (MQ=60)
                                           TTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCG                                                                                                       <  SRR19973070.1798545/150‑1 (MQ=60)
                                             TACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCC                                                                                                     <  SRR19973070.2225478/150‑1 (MQ=60)
                                             TACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCC                                                                                                     <  SRR19973070.2226703/150‑1 (MQ=60)
                                               CAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAG                                                                                                   >  SRR19973070.772660/1‑150 (MQ=60)
                                                     AACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCA                                                                                             <  SRR19973070.1921251/150‑1 (MQ=60)
                                                     AACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCA                                                                                             <  SRR19973070.1920162/150‑1 (MQ=60)
                                                         ATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACA                                                                                         >  SRR19973070.1299022/1‑150 (MQ=60)
                                                         ATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTGTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACA                                                                                         <  SRR19973070.2059009/150‑1 (MQ=60)
                                                           CACGACCGAGGAAGACTTTAATCATGCGTTAATTAATGAATGTTTTTGTGGTCAGTTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGAAATATATGTCCGGCCTTAATTAAGAACATAATCGCCAGGAGTCACACACT                                                                                       <  SRR19973070.1284955/150‑1 (MQ=60)
                                                           CACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACT                                                                                       >  SRR19973070.3185568/1‑150 (MQ=60)
                                                            ACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATTTTTTTGTGGTCAGTTTACTAATATATAGTTTTCGATTTTTAATATTTTTTGACGAGGCAATATATGTCCGGCCTTCATGAAGAAAATAATCGCCAGGAGTCACACACTG                                                                                      <  SRR19973070.565765/150‑1 (MQ=60)
                                                              GACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATACTCGCCAGGAGTCACACACTGAC                                                                                    <  SRR19973070.2601873/150‑1 (MQ=60)
                                                              GACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTGTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGAC                                                                                    >  SRR19973070.2701923/1‑150 (MQ=60)
                                                               ACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACG                                                                                   >  SRR19973070.20285/1‑150 (MQ=60)
                                                                 CGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTG                                                                                 <  SRR19973070.1212496/150‑1 (MQ=60)
                                                                  GAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGG                                                                                >  SRR19973070.1203887/1‑150 (MQ=60)
                                                                  GAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGG                                                                                >  SRR19973070.1202862/1‑150 (MQ=60)
                                                                  GAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTACGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGG                                                                                <  SRR19973070.1954946/150‑1 (MQ=60)
                                                                   AGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGT                                                                               >  SRR19973070.2332116/1‑150 (MQ=60)
                                                                   AGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTGTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGT                                                                               >  SRR19973070.117086/1‑150 (MQ=60)
                                                                      AAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGACCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATT                                                                            >  SRR19973070.1300928/1‑150 (MQ=60)
                                                                       AGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTT                                                                           <  SRR19973070.3138429/150‑1 (MQ=60)
                                                                       AGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTT                                                                           <  SRR19973070.760491/150‑1 (MQ=60)
                                                                       AGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTT                                                                           <  SRR19973070.760503/150‑1 (MQ=60)
                                                                       AGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTT                                                                           <  SRR19973070.1623909/150‑1 (MQ=60)
                                                                         ACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCA                                                                         <  SRR19973070.149/150‑1 (MQ=60)
                                                                         ACTTTAATCATGCGGTATGTAATTAATTTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGGTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCA                                                                         <  SRR19973070.2248479/150‑1 (MQ=60)
                                                                             TAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAG                                                                     <  SRR19973070.2276672/150‑1 (MQ=60)
                                                                             TAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAG                                                                     >  SRR19973070.592531/1‑150 (MQ=60)
                                                                             TAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAG                                                                     >  SRR19973070.592401/1‑150 (MQ=60)
                                                                              AATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGT                                                                    >  SRR19973070.3038429/1‑150 (MQ=60)
                                                                                        TCAGTAATGAATTTTTTTGTGGTCAGGTTACTAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGA                                                          <  SRR19973070.2336267/150‑1 (MQ=60)
                                                                                        TCAGTAATGAATGTTTTTGTGGTCAGTTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGA                                                          <  SRR19973070.1546078/150‑1 (MQ=60)
                                                                                          AGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATG                                                        >  SRR19973070.2816937/1‑150 (MQ=60)
                                                                                          AGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATG                                                        >  SRR19973070.1477780/1‑150 (MQ=60)
                                                                                          AGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATG                                                        >  SRR19973070.1477767/1‑150 (MQ=60)
                                                                                          AGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATG                                                        >  SRR19973070.1308178/1‑150 (MQ=60)
                                                                                              ATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCA                                                    >  SRR19973070.407929/1‑150 (MQ=60)
                                                                                              ATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCA                                                    >  SRR19973070.2326174/1‑150 (MQ=60)
                                                                                              ATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCA                                                    >  SRR19973070.653896/1‑150 (MQ=60)
                                                                                                 AATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTGTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATC                                                 <  SRR19973070.1905103/150‑1 (MQ=60)
                                                                                                   TGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCAT                                               >  SRR19973070.2653402/1‑150 (MQ=60)
                                                                                                   TGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCAT                                               >  SRR19973070.2652790/1‑150 (MQ=60)
                                                                                                   TGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCAT                                               >  SRR19973070.1008399/1‑150 (MQ=60)
                                                                                                      TTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAAT                                            <  SRR19973070.1115802/150‑1 (MQ=60)
                                                                                                       TTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGACGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATT                                           <  SRR19973070.106903/150‑1 (MQ=60)
                                                                                                       TTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATT                                           <  SRR19973070.106901/150‑1 (MQ=60)
                                                                                                          GTGGTCAGGTTACGAATTTATATTTTTCGATTTTTATTATTTTTTGCCGAGGCAATATTTGTCCGGCCTTCATTAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATC                                        <  SRR19973070.596927/150‑1 (MQ=60)
                                                                                                           TGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCT                                       <  SRR19973070.213374/150‑1 (MQ=60)
                                                                                                               CAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATATCCAT                                   >  SRR19973070.1719259/1‑150 (MQ=60)
                                                                                                                AGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATT                                  <  SRR19973070.2548704/150‑1 (MQ=60)
                                                                                                                 GGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGTAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTT                                 >  SRR19973070.774616/1‑150 (MQ=60)
                                                                                                                 GGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTT                                 >  SRR19973070.774669/1‑150 (MQ=60)
                                                                                                                 GGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTT                                 >  SRR19973070.774759/1‑150 (MQ=60)
                                                                                                                   TTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTT                               <  SRR19973070.99553/150‑1 (MQ=60)
                                                                                                                   TTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTT                               <  SRR19973070.99378/150‑1 (MQ=60)
                                                                                                                   TTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTACGAAGTGATGTGCAATCATAATTATCTCCATTTTT                               <  SRR19973070.2493855/150‑1 (MQ=60)
                                                                                                                   TTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTACGAAGTGATGTGCAATCATAATTATCTCCATTTTT                               <  SRR19973070.2493328/150‑1 (MQ=60)
                                                                                                                     ACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTAC                             <  SRR19973070.2479446/150‑1 (MQ=60)
                                                                                                                         ATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTagatcgg                         >  SRR19973070.1096305/1‑143 (MQ=60)
                                                                                                                         ATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAAT                         >  SRR19973070.2410801/1‑150 (MQ=60)
                                                                                                                         ATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAAT                         >  SRR19973070.2410821/1‑150 (MQ=60)
                                                                                                                           ATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGA                       <  SRR19973070.263101/150‑1 (MQ=60)
                                                                                                                           ATATAGTTTTCGCTTTTTAATATT‑TTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAA                      <  SRR19973070.1277628/150‑1 (MQ=60)
                                                                                                                           ATATAGTTTTCGCTTTTTAATATT‑TTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAA                      <  SRR19973070.2782054/150‑1 (MQ=60)
                                                                                                                             ATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGTACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAAC                     <  SRR19973070.2602009/150‑1 (MQ=60)
                                                                                                                             ATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAAC                     <  SRR19973070.1178004/150‑1 (MQ=60)
                                                                                                                             ATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAAC                     <  SRR19973070.2601361/150‑1 (MQ=60)
                                                                                                                                 TTTTCTTTTTTTAATATTTTTTTCCGAGGCAATTTATGTGCGGACTTCATTATTTAAATAATCGCCATGAGTCACACACTGACGTGGTATTTCATCAGTTCCGATGTGTTGTGCAATCATAATTATCTCCATTTTTACATATGAACTTTG                 <  SRR19973070.3076974/150‑1 (MQ=60)
                                                                                                                                 TTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTG                 <  SRR19973070.2568608/150‑1 (MQ=60)
                                                                                                                                 TTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCATTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTG                 <  SRR19973070.3076788/150‑1 (MQ=60)
                                                                                                                                 TTTTCGATTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTG                 <  SRR19973070.2568600/150‑1 (MQ=60)
                                                                                                                                   TTCGCTTTTTAATTTTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTT               <  SRR19973070.1893961/150‑1 (MQ=60)
                                                                                                                                      GCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGAT            >  SRR19973070.1355428/1‑150 (MQ=60)
                                                                                                                                         TTTTAATATTTTTTGCCGAGGCTATATATGTCCGGCATTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCG         <  SRR19973070.2137986/150‑1 (MQ=60)
                                                                                                                                         TTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCG         <  SRR19973070.2137973/150‑1 (MQ=60)
                                                                                                                                                ATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGTGTCTG  >  SRR19973070.465732/1‑150 (MQ=60)
                                                                                                                                                ATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGTGTCTG  >  SRR19973070.465710/1‑150 (MQ=60)
                                                                                                                                                ATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGTGTCTG  >  SRR19973070.434587/1‑150 (MQ=60)
                                                                                                                                                ATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGTGTCTG  >  SRR19973070.2498889/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                  
GGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTGTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGTGTCTG  >  CP009273/1412238‑1412531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑