Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,412,385 G→T 100% N34K (AAC→AAA)  kilR ← Rac prophage; inhibitor of ftsZ, killing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,412,3850GT97.6% 820.4 / 8.4 257N34K (AAC→AAA) kilRRac prophage; inhibitor of ftsZ, killing protein
Reads supporting (aligned to +/- strand):  ref base G (6/1);  new base T (127/123);  total (133/124)
Fisher's exact test for biased strand distribution p-value = 1.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 261 total aligned reads displayed.

GGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTGTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGTGTCTG  >  CP009273/1412238‑1412531
                                                                                                                                                   |                                                                                                                                                  
ggtAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATtttt                                                                                                                                                  <  2:2736088/150‑1 (MQ=255)
 gtAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATAtttttt                                                                                                                                                 <  2:4084215/150‑1 (MQ=255)
         attttattttCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGc                                                                                                                                         >  1:2394535/1‑150 (MQ=255)
            ttattttCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAat                                                                                                                                      <  1:127653/150‑1 (MQ=255)
             tattttCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAata                                                                                                                                     <  2:3997786/150‑1 (MQ=255)
               ttttCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAatata                                                                                                                                   >  1:1430720/1‑150 (MQ=255)
               ttttCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAatata                                                                                                                                   <  2:3275684/150‑1 (MQ=255)
                        tcatcaCCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGcc                                                                                                                          >  2:587206/1‑150 (MQ=255)
                         catcaCCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCt                                                                                                                         >  1:4703215/1‑150 (MQ=255)
                          atcaCCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCtt                                                                                                                        >  2:675555/1‑150 (MQ=255)
                          atcaCCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCtt                                                                                                                        <  1:4143260/150‑1 (MQ=255)
                          atcaCCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCtt                                                                                                                        >  1:57731/1‑150 (MQ=255)
                           tcaCCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTc                                                                                                                       <  2:1077420/150‑1 (MQ=255)
                              ccATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATg                                                                                                                    >  1:61680/1‑150 (MQ=255)
                              ccATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATg                                                                                                                    >  1:1436680/1‑150 (MQ=255)
                              ccATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATg                                                                                                                    <  2:1634798/150‑1 (MQ=255)
                              ccATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATg                                                                                                                    >  1:1237087/1‑150 (MQ=255)
                               cATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATga                                                                                                                   >  2:1723824/1‑150 (MQ=255)
                               cATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTGTTTAATATTTTTTGACGAGGCAAAATATGTCCGGCCTTCATga                                                                                                                   >  1:3601905/1‑150 (MQ=255)
                                aTGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATgaa                                                                                                                  >  2:1846107/1‑150 (MQ=255)
                                  gACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATgaaga                                                                                                                <  2:1768900/150‑1 (MQ=255)
                                    cTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAAc                                                                                                              <  1:3028551/150‑1 (MQ=255)
                                     tactccTTTACAGTTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCATTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCATTCATGAAGAACa                                                                                                             <  1:1642502/146‑1 (MQ=255)
                                      ccGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACAt                                                                                                            >  2:120716/1‑150 (MQ=255)
                                      ccGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACAt                                                                                                            >  1:3489345/1‑150 (MQ=255)
                                         ccTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAAt                                                                                                         <  2:3559255/150‑1 (MQ=255)
                                          cTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATc                                                                                                        <  2:4286520/150‑1 (MQ=255)
                                           tttACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCg                                                                                                       <  2:4270276/150‑1 (MQ=255)
                                           tttACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCg                                                                                                       <  1:1315009/150‑1 (MQ=255)
                                           tttACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCg                                                                                                       <  2:4270256/150‑1 (MQ=255)
                                            ttACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGc                                                                                                      >  1:758505/1‑150 (MQ=255)
                                              aCAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCa                                                                                                    >  2:5064101/1‑150 (MQ=255)
                                               cAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAg                                                                                                   >  1:4364930/1‑150 (MQ=255)
                                                aGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAgg                                                                                                  <  2:1230432/150‑1 (MQ=255)
                                                   tAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGt                                                                                               <  2:5142423/150‑1 (MQ=255)
                                                     aaCCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTca                                                                                             <  2:779819/150‑1 (MQ=255)
                                                     aaCCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTca                                                                                             <  2:1379235/150‑1 (MQ=255)
                                                       ccATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAAc                                                                                                              <  2:612619/131‑1 (MQ=255)
                                                        cATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTcacac                                                                                          >  1:5043975/1‑150 (MQ=255)
                                                          tCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCacacac                                                                                        >  1:983958/1‑150 (MQ=255)
                                                              gACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGAc                                                                                    >  1:3322114/1‑150 (MQ=255)
                                                               aCCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACg                                                                                   <  1:3258790/150‑1 (MQ=255)
                                                                ccGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGt                                                                                  <  2:3695780/150‑1 (MQ=255)
                                                                 cGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTg                                                                                 >  2:2340300/1‑150 (MQ=255)
                                                                  gAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTgg                                                                                >  1:2244333/1‑150 (MQ=255)
                                                                      aaGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTAtt                                                                            >  2:3365895/1‑150 (MQ=255)
                                                                       aGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCAGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTAttt                                                                           >  2:150297/1‑150 (MQ=255)
                                                                        gACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTtc                                                                          <  2:2058760/150‑1 (MQ=255)
                                                                        gACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTtc                                                                          <  1:2977273/150‑1 (MQ=255)
                                                                         aCTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTtca                                                                         <  1:2011426/150‑1 (MQ=255)
                                                                          cTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTtcat                                                                        <  2:422197/150‑1 (MQ=255)
                                                                           tttAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTtcatc                                                                       >  2:3133413/1‑150 (MQ=255)
                                                                            ttAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTtcatca                                                                      >  1:2502969/1‑150 (MQ=255)
                                                                            ttAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTtcatca                                                                      <  1:1750206/150‑1 (MQ=255)
                                                                             tAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAg                                                                     <  2:4772896/150‑1 (MQ=255)
                                                                             tAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAg                                                                     <  2:4772969/150‑1 (MQ=255)
                                                                             tAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAg                                                                     <  2:2870991/150‑1 (MQ=255)
                                                                              aaTCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTATTTAATATATTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGACACACACTGACGTGGTATATCATCAGt                                                                    >  1:2580749/1‑150 (MQ=255)
                                                                                tCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCTTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTc                                                                  <  1:3872834/150‑1 (MQ=255)
                                                                                tCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTc                                                                  <  1:74060/150‑1 (MQ=255)
                                                                                   tGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTGTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGa                                                               >  1:1829058/1‑150 (MQ=255)
                                                                                    gCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGaa                                                              >  2:4571262/1‑150 (MQ=255)
                                                                                      ggTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTGGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGt                                                            <  1:516894/150‑1 (MQ=255)
                                                                                       gTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTg                                                           <  2:2707258/150‑1 (MQ=255)
                                                                                          aGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGAtg                                                        <  2:2479336/150‑1 (MQ=255)
                                                                                           gTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGAtgt                                                       <  2:2394535/150‑1 (MQ=255)
                                                                                            tAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGAtgtg                                                      <  1:879291/150‑1 (MQ=255)
                                                                                            tAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGAtgtg                                                      <  1:3540723/150‑1 (MQ=255)
                                                                                               tgaatgTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCaa                                                   >  1:1881925/1‑150 (MQ=255)
                                                                                                 aatgTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATc                                                 <  1:3812546/150‑1 (MQ=255)
                                                                                                     tttttGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATAGGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATaa                                             <  1:901081/150‑1 (MQ=255)
                                                                                                        ttGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTa                                          <  1:4487056/150‑1 (MQ=255)
                                                                                                        ttGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTa                                          <  1:3838692/150‑1 (MQ=255)
                                                                                                         tgtgGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTAt                                         <  1:1587622/150‑1 (MQ=255)
                                                                                                            ggTCAGGTTACGAATATATAGTTATCGCTTTTTAAAATTTTTTGCCGAGTCAATATATGTCCGGCCTTCATGAAGAACATAATAGACAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTAtatc                                      >  2:857004/1‑150 (MQ=255)
                                                                                                             gTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTcc                                     <  2:2010201/150‑1 (MQ=255)
                                                                                                               cAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCAt                                   >  2:2438049/1‑150 (MQ=255)
                                                                                                                 ggTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCAttt                                 <  1:1831948/150‑1 (MQ=255)
                                                                                                                    tACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTa                              <  1:228805/150‑1 (MQ=255)
                                                                                                                       gAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACaa                           >  2:2050262/1‑150 (MQ=255)
                                                                                                                           atataGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGa                       <  2:896451/150‑1 (MQ=255)
                                                                                                                           atataGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGa                       <  1:1721481/150‑1 (MQ=255)
                                                                                                                             ataGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAAc                     >  2:3005922/1‑150 (MQ=255)
                                                                                                                             ataGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAAc                     >  1:2018676/1‑150 (MQ=255)
                                                                                                                              taGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACt                    <  1:1444355/150‑1 (MQ=255)
                                                                                                                                 ttttCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTttg                 >  2:4531539/1‑150 (MQ=255)
                                                                                                                                  tttCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTttgt                <  2:1284404/150‑1 (MQ=255)
                                                                                                                                     cGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGa             <  1:844801/150‑1 (MQ=255)
                                                                                                                                      gCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGAt            <  2:1282301/150‑1 (MQ=255)
                                                                                                                                       cTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATg           >  1:2188279/1‑150 (MQ=255)
                                                                                                                                        tttttAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGc          >  2:148859/1‑150 (MQ=255)
                                                                                                                                        tttttAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGc          <  1:479979/150‑1 (MQ=255)
                                                                                                                                         ttttAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCg         <  2:2227964/150‑1 (MQ=255)
                                                                                                                                          tttAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCgg        >  1:4233260/1‑150 (MQ=255)
                                                                                                                                           ttAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGgt       >  1:1131350/1‑150 (MQ=255)
                                                                                                                                            tAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGgtg      >  1:1046813/1‑150 (MQ=255)
                                                                                                                                             aaTATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGgtgt     <  2:4906767/150‑1 (MQ=255)
                                                                                                                                               tatTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGTGTCt   >  1:944912/1‑150 (MQ=255)
                                                                                                                                               tatTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGTGTCt   <  2:1641400/150‑1 (MQ=255)
                                                                                                                                                atTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGTGTCTg  >  2:2758851/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTGTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGTGTCTG  >  CP009273/1412238‑1412531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTGTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGTGTCTGGT  >  CP009273/1412236‑1412533
                                                                                                                                                     |                                                                                                                                                    
GTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTT                                                                                                                                                      >  SRR19973063.933806/1‑150 (MQ=60)
GTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTT                                                                                                                                                      >  SRR19973063.2016479/1‑150 (MQ=60)
GTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTT                                                                                                                                                      >  SRR19973063.933811/1‑150 (MQ=60)
 TGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTT                                                                                                                                                     >  SRR19973063.108138/1‑150 (MQ=60)
 TGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTT                                                                                                                                                     <  SRR19973063.19003/150‑1 (MQ=60)
 TGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTT                                                                                                                                                     >  SRR19973063.4983121/1‑150 (MQ=60)
   GTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTT                                                                                                                                                   >  SRR19973063.861853/1‑150 (MQ=60)
      AGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCC                                                                                                                                                >  SRR19973063.555401/1‑150 (MQ=60)
          CATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGG                                                                                                                                            >  SRR19973063.3405331/1‑150 (MQ=60)
           ATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGC                                                                                                                                           >  SRR19973063.2402197/1‑150 (MQ=60)
              TTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAAT                                                                                                                                        <  SRR19973063.128066/150‑1 (MQ=60)
                ATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATAT                                                                                                                                      <  SRR19973063.1321246/150‑1 (MQ=60)
                 TTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATA                                                                                                                                     >  SRR19973063.1435083/1‑150 (MQ=60)
                 TTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATA                                                                                                                                     >  SRR19973063.1435087/1‑150 (MQ=60)
                       TAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCG                                                                                                                               <  SRR19973063.835920/150‑1 (MQ=60)
                           CATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCT                                                                                                                           >  SRR19973063.1288229/1‑150 (MQ=60)
                           CATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCT                                                                                                                           >  SRR19973063.2259722/1‑150 (MQ=60)
                           CATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCT                                                                                                                           >  SRR19973063.1359792/1‑150 (MQ=60)
                           CATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCT                                                                                                                           >  SRR19973063.4718204/1‑150 (MQ=60)
                           CATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCT                                                                                                                           >  SRR19973063.3954048/1‑150 (MQ=60)
                            ATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTT                                                                                                                          <  SRR19973063.4156513/150‑1 (MQ=60)
                            ATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTT                                                                                                                          >  SRR19973063.57934/1‑150 (MQ=60)
                            ATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTT                                                                                                                          <  SRR19973063.1710397/150‑1 (MQ=60)
                                CCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATG                                                                                                                      >  SRR19973063.1240600/1‑150 (MQ=60)
                                CCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATG                                                                                                                      >  SRR19973063.1240776/1‑150 (MQ=60)
                                CCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATG                                                                                                                      >  SRR19973063.1441064/1‑150 (MQ=60)
                                CCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATG                                                                                                                      >  SRR19973063.61891/1‑150 (MQ=60)
                                 CATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGA                                                                                                                     >  SRR19973063.3825129/1‑150 (MQ=60)
                                 CATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTGTTTAATATTTTTTGACGAGGCAAAATATGTCCGGCCTTCATGA                                                                                                                     >  SRR19973063.3613462/1‑150 (MQ=60)
                                      CTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAAC                                                                                                                <  SRR19973063.3038212/150‑1 (MQ=60)
                                      CTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCAGGCCTTCATGAAGAAC                                                                                                                >  SRR19973063.4387804/1‑150 (MQ=60)
                                       tactCCTTTACAGTTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCATTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCATTCATGAAGAACA                                                                                                               <  SRR19973063.1647525/146‑1 (MQ=60)
                                        CCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACAT                                                                                                              >  SRR19973063.3500495/1‑150 (MQ=60)
                                        CCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACAT                                                                                                              >  SRR19973063.1612805/1‑150 (MQ=60)
                                            CTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATC                                                                                                          <  SRR19973063.4974611/150‑1 (MQ=60)
                                             TTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCG                                                                                                         <  SRR19973063.1318933/150‑1 (MQ=60)
                                              TTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGC                                                                                                        >  SRR19973063.760715/1‑150 (MQ=60)
                                              TTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGC                                                                                                        >  SRR19973063.760726/1‑150 (MQ=60)
                                                ACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCA                                                                                                      <  SRR19973063.1935246/150‑1 (MQ=60)
                                                ACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCA                                                                                                      >  SRR19973063.1309311/1‑150 (MQ=60)
                                                 CAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAG                                                                                                     >  SRR19973063.4378893/1‑150 (MQ=60)
                                                       AACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCA                                                                                               <  SRR19973063.3507126/150‑1 (MQ=60)
                                                         CCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACAgatcggaagagcacacgt                                                                                             >  SRR19973063.614419/1‑132 (MQ=60)
                                                          CATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACAC                                                                                            >  SRR19973063.5060225/1‑150 (MQ=60)
                                                            TCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACAC                                                                                          >  SRR19973063.987919/1‑150 (MQ=60)
                                                            TCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACAC                                                                                          >  SRR19973063.986927/1‑150 (MQ=60)
                                                                GACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGAC                                                                                      >  SRR19973063.3332721/1‑150 (MQ=60)
                                                                GACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGAC                                                                                      >  SRR19973063.3332724/1‑150 (MQ=60)
                                                                GACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGAC                                                                                      >  SRR19973063.3332828/1‑150 (MQ=60)
                                                                 ACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACG                                                                                     <  SRR19973063.3269251/150‑1 (MQ=60)
                                                                   CGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTG                                                                                   >  SRR19973063.3608125/1‑150 (MQ=60)
                                                                    GAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGG                                                                                  >  SRR19973063.2251461/1‑150 (MQ=60)
                                                                    GAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGG                                                                                  >  SRR19973063.2251485/1‑150 (MQ=60)
                                                                    GAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGG                                                                                  >  SRR19973063.514990/1‑150 (MQ=60)
                                                                    GAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGG                                                                                  >  SRR19973063.514829/1‑150 (MQ=60)
                                                                     AGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGT                                                                                 <  SRR19973063.3540407/150‑1 (MQ=60)
                                                                     AGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGT                                                                                 <  SRR19973063.2135066/150‑1 (MQ=60)
                                                                      GGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTA                                                                                >  SRR19973063.1100243/1‑150 (MQ=60)
                                                                          GACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGTTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGAAATATTTGTCCGGCCTTCATTAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTC                                                                            <  SRR19973063.2329925/150‑1 (MQ=60)
                                                                          GACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTC                                                                            <  SRR19973063.2986787/150‑1 (MQ=60)
                                                                           ACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATTTAGTTTTCGCTTTTTAATATTTTTTGCCGAGTCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCA                                                                           <  SRR19973063.2017750/150‑1 (MQ=60)
                                                                           ACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCA                                                                           <  SRR19973063.2017707/150‑1 (MQ=60)
                                                                              TTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACTAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCA                                                                        <  SRR19973063.1755553/150‑1 (MQ=60)
                                                                              TTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCA                                                                        >  SRR19973063.2510951/1‑150 (MQ=60)
                                                                              TTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCA                                                                        <  SRR19973063.1755571/150‑1 (MQ=60)
                                                                              TTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCA                                                                        >  SRR19973063.2510931/1‑150 (MQ=60)
                                                                               TAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAG                                                                       >  SRR19973063.3671129/1‑150 (MQ=60)
                                                                                AATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTATTTAATATATTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGACACACACTGACGTGGTATATCATCAGT                                                                      >  SRR19973063.2588955/1‑150 (MQ=60)
                                                                                  TCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCTTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTC                                                                    <  SRR19973063.3885142/150‑1 (MQ=60)
                                                                                  TCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCATGAGTCACACACTGACGTGGTATTTCATCAGTTC                                                                    >  SRR19973063.1611581/1‑150 (MQ=60)
                                                                                  TCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTC                                                                    <  SRR19973063.74317/150‑1 (MQ=60)
                                                                                  TCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTC                                                                    <  SRR19973063.3885036/150‑1 (MQ=60)
                                                                                  TCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTC                                                                    >  SRR19973063.4202318/1‑150 (MQ=60)
                                                                                    ATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCG                                                                  <  SRR19973063.3027712/150‑1 (MQ=60)
                                                                                     TGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTGTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGA                                                                 >  SRR19973063.1834750/1‑150 (MQ=60)
                                                                                     TGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTGTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGA                                                                 >  SRR19973063.1834749/1‑150 (MQ=60)
                                                                                        GGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGT                                                              >  SRR19973063.4642524/1‑150 (MQ=60)
                                                                                        GGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGT                                                              >  SRR19973063.4642565/1‑150 (MQ=60)
                                                                                        GGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTGGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGT                                                              <  SRR19973063.518402/150‑1 (MQ=60)
                                                                                         GTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTG                                                             >  SRR19973063.144325/1‑150 (MQ=60)
                                                                                         GTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTG                                                             >  SRR19973063.144301/1‑150 (MQ=60)
                                                                                           CAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGAT                                                           >  SRR19973063.2195045/1‑150 (MQ=60)
                                                                                            AGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATG                                                          <  SRR19973063.4314786/150‑1 (MQ=60)
                                                                                            AGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATG                                                          <  SRR19973063.4314802/150‑1 (MQ=60)
                                                                                              TAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTG                                                        <  SRR19973063.3552068/150‑1 (MQ=60)
                                                                                              TAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTG                                                        <  SRR19973063.881827/150‑1 (MQ=60)
                                                                                              TAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTG                                                        <  SRR19973063.760599/150‑1 (MQ=60)
                                                                                               AATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGC                                                       >  SRR19973063.1772260/1‑150 (MQ=60)
                                                                                                 TGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAA                                                     >  SRR19973063.1887807/1‑150 (MQ=60)
                                                                                                   AATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATC                                                   <  SRR19973063.3824680/150‑1 (MQ=60)
                                                                                                    ATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTTCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCA                                                  >  SRR19973063.996698/1‑150 (MQ=60)
                                                                                                       TTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAA                                               <  SRR19973063.2557003/150‑1 (MQ=60)
                                                                                                       TTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAA                                               <  SRR19973063.903699/150‑1 (MQ=60)
                                                                                                       TTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATAGGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAA                                               <  SRR19973063.903712/150‑1 (MQ=60)
                                                                                                          TTGTGGTCTGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACAATGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTA                                            <  SRR19973063.1262760/150‑1 (MQ=60)
                                                                                                          TTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTA                                            <  SRR19973063.3850897/150‑1 (MQ=60)
                                                                                                          TTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTA                                            <  SRR19973063.4501444/150‑1 (MQ=60)
                                                                                                          TTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTATAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTA                                            >  SRR19973063.1871746/1‑150 (MQ=60)
                                                                                                           TGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTAT                                           <  SRR19973063.1592486/150‑1 (MQ=60)
                                                                                                             TGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCT                                         <  SRR19973063.1647965/150‑1 (MQ=60)
                                                                                                              GGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTC                                        >  SRR19973063.224477/1‑150 (MQ=60)
                                                                                                               GTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCC                                       <  SRR19973063.677563/150‑1 (MQ=60)
                                                                                                               GTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCC                                       <  SRR19973063.677529/150‑1 (MQ=60)
                                                                                                                TCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCA                                      >  SRR19973063.3710267/1‑150 (MQ=60)
                                                                                                                 CAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCAT                                     <  SRR19973063.2523943/150‑1 (MQ=60)
                                                                                                                   GGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTT                                   <  SRR19973063.1837668/150‑1 (MQ=60)
                                                                                                                   GGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTT                                   <  SRR19973063.1837649/150‑1 (MQ=60)
                                                                                                                   GGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTT                                   <  SRR19973063.1837583/150‑1 (MQ=60)
                                                                                                                   GGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTT                                   >  SRR19973063.521115/1‑150 (MQ=60)
                                                                                                                      TACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTA                                <  SRR19973063.229490/150‑1 (MQ=60)
                                                                                                                        CGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACA                              >  SRR19973063.1494758/1‑150 (MQ=60)
                                                                                                                            TATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATG                          >  SRR19973063.2352925/1‑150 (MQ=60)
                                                                                                                             ATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGA                         <  SRR19973063.1726731/150‑1 (MQ=60)
                                                                                                                               ATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAAC                       >  SRR19973063.2024977/1‑150 (MQ=60)
                                                                                                                                TAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACT                      >  SRR19973063.190663/1‑150 (MQ=60)
                                                                                                                                TAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACT                      <  SRR19973063.1448763/150‑1 (MQ=60)
                                                                                                                                 agaaTTCGCTTTTTAATATTGTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAAAAATCTACAATTTTACAAAAGAACTT                     >  SRR19973063.4793744/5‑150 (MQ=60)
                                                                                                                                 AGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTT                     <  SRR19973063.3221758/150‑1 (MQ=60)
                                                                                                                                 AGTTTTCGCTTTTTAATATTGTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTT                     >  SRR19973063.4794129/1‑150 (MQ=60)
                                                                                                                                  GTTTTCGCTTTTTAATATTGTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTT                    >  SRR19973063.2886261/1‑150 (MQ=60)
                                                                                                                                      TCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTG                <  SRR19973063.908783/150‑1 (MQ=60)
                                                                                                                                       CGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGA               <  SRR19973063.847240/150‑1 (MQ=60)
                                                                                                                                       CGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGA               <  SRR19973063.847247/150‑1 (MQ=60)
                                                                                                                                         CTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATG             >  SRR19973063.2195144/1‑150 (MQ=60)
                                                                                                                                          TTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGC            <  SRR19973063.481342/150‑1 (MQ=60)
                                                                                                                                            TTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGG          >  SRR19973063.4246752/1‑150 (MQ=60)
                                                                                                                                            TTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGG          >  SRR19973063.386297/1‑150 (MQ=60)
                                                                                                                                             TTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGT         >  SRR19973063.1138606/1‑150 (MQ=60)
                                                                                                                                             TTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGT         >  SRR19973063.1134784/1‑150 (MQ=60)
                                                                                                                                              TAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGTG        >  SRR19973063.3156283/1‑150 (MQ=60)
                                                                                                                                              TAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGTG        >  SRR19973063.1049984/1‑150 (MQ=60)
                                                                                                                                                 TATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGTGTCT     >  SRR19973063.2646005/1‑150 (MQ=60)
                                                                                                                                                 TATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGTGTCT     >  SRR19973063.947627/1‑150 (MQ=60)
                                                                                                                                                 TATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGTGTCT     >  SRR19973063.947739/1‑150 (MQ=60)
                                                                                                                                                 TATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGTGTCT     >  SRR19973063.947760/1‑150 (MQ=60)
                                                                                                                                                 TATTGTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGTGTCT     >  SRR19973063.3989616/1‑150 (MQ=60)
                                                                                                                                                    TTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGTGTCTGGT  <  SRR19973063.3119635/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
GTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTGTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGTGTCTGGT  >  CP009273/1412236‑1412533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑