Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,614,089 G→T 88.8% E87* (GAG→TAG)  marA → multiple antibiotic resistance transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,614,0890GT88.8% 546.5 / 65.8 221E87* (GAG→TAG) marAmultiple antibiotic resistance transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (15/10);  new base T (104/92);  total (119/102)
Fisher's exact test for biased strand distribution p-value = 5.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 222 total aligned reads displayed.

TCCAAATGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTC  >  CP009273/1613942‑1614233
                                                                                                                                                   |                                                                                                                                                
tCCAAATGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCg                                                                                                                                                  >  1:2199631/1‑148 (MQ=255)
  cAAATGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAg                                                                                                                                                >  2:28902/1‑148 (MQ=255)
      tGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGc                                                                                                                                            >  1:1935738/1‑148 (MQ=255)
           ccTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGcaacaa                                                                                                                                       >  2:1270759/1‑148 (MQ=255)
           ccTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGcaacaa                                                                                                                                       >  2:3113618/1‑148 (MQ=255)
            cTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACaaa                                                                                                                                      <  2:882700/148‑1 (MQ=255)
            cTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACaaa                                                                                                                                      <  2:2205189/148‑1 (MQ=255)
               cAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAActc                                                                                                                                   <  2:3250102/148‑1 (MQ=255)
                 aCGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTg                                                                                                                                 >  1:917936/1‑148 (MQ=255)
                  cGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGa                                                                                                                                >  1:560543/1‑148 (MQ=255)
                    gATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGAcc                                                                                                                              >  1:3194706/1‑148 (MQ=255)
                      tGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGATAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCg                                                                                                                            <  1:2802651/148‑1 (MQ=255)
                      tGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCg                                                                                                                            >  1:858633/1‑148 (MQ=255)
                        tttAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGaa                                                                                                                          >  1:689698/1‑148 (MQ=255)
                        tttAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGaa                                                                                                                          >  2:203385/1‑148 (MQ=255)
                             aaaaGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTc                                                                                                                     >  2:1446175/1‑148 (MQ=255)
                                aGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCaaa                                                                                                                  >  2:1979807/1‑148 (MQ=255)
                                    aCCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAAtt                                                                                                              >  1:243779/1‑148 (MQ=255)
                                       ggTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACt                                                                                                           <  2:1152484/148‑1 (MQ=255)
                                       ggTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACt                                                                                                           <  1:1619398/148‑1 (MQ=255)
                                       ggTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACt                                                                                                           <  2:1718934/148‑1 (MQ=255)
                                        gTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACtt                                                                                                          <  2:2440353/148‑1 (MQ=255)
                                         tcattcatTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACttt                                                                                                         >  1:1727243/1‑148 (MQ=255)
                                         tcattcatTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACttt                                                                                                         <  1:1166094/148‑1 (MQ=255)
                                           attcattAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGa                                                                                                       <  1:1374164/148‑1 (MQ=255)
                                            ttcattAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGAt                                                                                                      <  2:166751/148‑1 (MQ=255)
                                            ttcattAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGAt                                                                                                      >  1:1830494/1‑148 (MQ=255)
                                             tcattAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATg                                                                                                     >  2:673358/1‑148 (MQ=255)
                                             tcattAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATg                                                                                                     <  1:63823/148‑1 (MQ=255)
                                                ttAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTc                                                                                                  >  1:2361277/1‑148 (MQ=255)
                                                 tAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTcc                                                                                                 >  1:1546624/1‑148 (MQ=255)
                                                       aaTACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCa                                                                                           <  2:519473/148‑1 (MQ=255)
                                                       aaTACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCa                                                                                           >  2:2135970/1‑148 (MQ=255)
                                                       aaTACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCa                                                                                           <  2:1122754/148‑1 (MQ=255)
                                                       aaTACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCa                                                                                           >  2:2136424/1‑148 (MQ=255)
                                                        aTACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCAt                                                                                          >  2:1047959/1‑148 (MQ=255)
                                                        aTACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCAt                                                                                          >  2:2465159/1‑148 (MQ=255)
                                                        aTACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCAt                                                                                          >  2:254469/1‑148 (MQ=255)
                                                         tACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATa                                                                                         >  1:2449031/1‑148 (MQ=255)
                                                         tACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATa                                                                                         >  1:1851385/1‑148 (MQ=255)
                                                         tACATCCCCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTCTCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATa                                                                                         >  2:844077/1‑148 (MQ=255)
                                                          aCATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATaa                                                                                        <  2:3105615/148‑1 (MQ=255)
                                                          aCATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATaa                                                                                        <  2:3105792/148‑1 (MQ=255)
                                                          aCATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATaa                                                                                        >  1:3104641/1‑148 (MQ=255)
                                                            aTCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAAt                                                                                      >  2:3148316/1‑148 (MQ=255)
                                                             tCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATa                                                                                     <  2:1908669/148‑1 (MQ=255)
                                                              ccGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGACAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATAc                                                                                    >  2:441539/1‑148 (MQ=255)
                                                              ccGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATAc                                                                                    <  1:3062831/148‑1 (MQ=255)
                                                                 cAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCgg                                                                                 <  2:2870665/148‑1 (MQ=255)
                                                                 cAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCgg                                                                                 <  1:1974669/148‑1 (MQ=255)
                                                                     cGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGa                                                                             <  1:2157405/148‑1 (MQ=255)
                                                                        aaGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCa                                                                          >  2:2511995/1‑148 (MQ=255)
                                                                           aTGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAata                                                                       <  1:1294726/148‑1 (MQ=255)
                                                                           aTGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAata                                                                       <  2:1293885/148‑1 (MQ=255)
                                                                             gACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATg                                                                     <  1:199244/148‑1 (MQ=255)
                                                                             gACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATg                                                                     <  1:2869120/148‑1 (MQ=255)
                                                                              aCGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGc                                                                    <  1:822779/148‑1 (MQ=255)
                                                                               cGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCa                                                                   <  2:2133152/148‑1 (MQ=255)
                                                                                    aTCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCg                                                              >  1:699359/1‑148 (MQ=255)
                                                                                      cgcgCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGaa                                                            <  1:1565387/148‑1 (MQ=255)
                                                                                            aaaGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCgcgc                                                      <  2:1087192/148‑1 (MQ=255)
                                                                                             aaGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCt                                                     <  2:157853/148‑1 (MQ=255)
                                                                                             aaGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCt                                                     <  1:388385/148‑1 (MQ=255)
                                                                                               gCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCttt                                                   >  1:2871574/1‑148 (MQ=255)
                                                                                                cTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCtttt                                                  >  2:2690613/1‑148 (MQ=255)
                                                                                                 tGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCttttt                                                 <  1:2258019/148‑1 (MQ=255)
                                                                                                 tGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCttttt                                                 >  2:2229798/1‑148 (MQ=255)
                                                                                                  gAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTa                                                >  2:1749738/1‑148 (MQ=255)
                                                                                                   aaGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTAc                                               <  1:469786/148‑1 (MQ=255)
                                                                                                   aaGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTAc                                               <  2:2185259/148‑1 (MQ=255)
                                                                                                   aaGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTAc                                               >  1:2711066/1‑148 (MQ=255)
                                                                                                      gAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATc                                            >  2:758735/1‑148 (MQ=255)
                                                                                                       aaaGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATcc                                           >  2:1098684/1‑148 (MQ=255)
                                                                                                       aaaCTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATcc                                           <  2:3045853/148‑1 (MQ=255)
                                                                                                         aGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCAt                                         >  1:2923840/1‑148 (MQ=255)
                                                                                                           tAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTa                                       <  2:378405/148‑1 (MQ=255)
                                                                                                            aaCGAGCCGATACTCTATCTGGCAGAGCGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTaa                                      <  2:858678/148‑1 (MQ=255)
                                                                                                             aCGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTaaa                                     <  1:2075625/148‑1 (MQ=255)
                                                                                                             aCGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTaaa                                     <  1:409662/148‑1 (MQ=255)
                                                                                                              cGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAAt                                    <  2:1553262/148‑1 (MQ=255)
                                                                                                                 gCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCAt                                 <  2:1685133/148‑1 (MQ=255)
                                                                                                                       aCTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTacaac                           >  2:1935166/1‑148 (MQ=255)
                                                                                                                       aCTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTacaac                           >  1:788870/1‑148 (MQ=255)
                                                                                                                         tctATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAg                         <  2:3015220/148‑1 (MQ=255)
                                                                                                                         tctATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAg                         <  2:720485/148‑1 (MQ=255)
                                                                                                                          ctATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGc                        >  2:2397600/1‑148 (MQ=255)
                                                                                                                            aTCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTa                      >  1:1489698/1‑148 (MQ=255)
                                                                                                                                ggCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTg                  >  1:3305547/1‑148 (MQ=255)
                                                                                                                                ggCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTg                  >  1:1243015/1‑148 (MQ=255)
                                                                                                                                  cAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGaa                >  2:110424/1‑148 (MQ=255)
                                                                                                                                    gAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGaaaa              >  1:1101001/1‑148 (MQ=255)
                                                                                                                                    gAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGaaaa              >  2:210593/1‑148 (MQ=255)
                                                                                                                                     aaCGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAAc             >  1:980829/1‑148 (MQ=255)
                                                                                                                                     aaCGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAAc             >  2:912544/1‑148 (MQ=255)
                                                                                                                                     aaCGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAAc             <  1:101240/148‑1 (MQ=255)
                                                                                                                                       cGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGt           <  2:2330616/148‑1 (MQ=255)
                                                                                                                                        gATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTg          <  2:3235543/148‑1 (MQ=255)
                                                                                                                                              gCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAAGACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACg    <  2:1007535/148‑1 (MQ=255)
                                                                                                                                                ttCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTc  <  2:36047/148‑1 (MQ=255)
                                                                                                                                                ttCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTc  <  1:3232022/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
TCCAAATGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTC  >  CP009273/1613942‑1614233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACTCCAAATGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACT  >  CP009273/1613940‑1614236
                                                                                                                                                     |                                                                                                                                                   
ACTCCAAATGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCT                                                                                                                                                     <  SRR19973070.2429944/150‑1 (MQ=60)
ACTCCAAATGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCT                                                                                                                                                     >  SRR19973070.168112/1‑150 (MQ=60)
ACTCCAAATGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCG                                                                                                                                                     >  SRR19973070.2214134/1‑150 (MQ=60)
    CAAATGGCACCTGCAACGGATTTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAACTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTC                                                                                                                                                 <  SRR19973070.6875/150‑1 (MQ=60)
      AATGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGC                                                                                                                                               >  SRR19973070.1948755/1‑150 (MQ=60)
                 CAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTG                                                                                                                                    >  SRR19973070.924470/1‑150 (MQ=60)
                  AACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGA                                                                                                                                   >  SRR19973070.564241/1‑150 (MQ=60)
                    CGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACC                                                                                                                                 >  SRR19973070.3215379/1‑150 (MQ=60)
                    CGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACC                                                                                                                                 >  SRR19973070.3215715/1‑150 (MQ=60)
                    CGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACC                                                                                                                                 >  SRR19973070.2889262/1‑150 (MQ=60)
                      GATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAGCGATATGGCTTCTAGTCGCAACAAACTCTGACCCG                                                                                                                               >  SRR19973070.864628/1‑150 (MQ=60)
                      GATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCG                                                                                                                               >  SRR19973070.864582/1‑150 (MQ=60)
                        TGTTTAAAATAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGATAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAA                                                                                                                             <  SRR19973070.2820759/150‑1 (MQ=60)
                        TGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAA                                                                                                                             >  SRR19973070.694619/1‑150 (MQ=60)
                        TGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAA                                                                                                                             <  SRR19973070.2821013/150‑1 (MQ=60)
                         GTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAAC                                                                                                                            >  SRR19973070.3256428/1‑150 (MQ=60)
                            TAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTT                                                                                                                         >  SRR19973070.566041/1‑150 (MQ=60)
                            TAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTT                                                                                                                         >  SRR19973070.1648888/1‑150 (MQ=60)
                              CAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGACAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCA                                                                                                                       <  SRR19973070.3140068/150‑1 (MQ=60)
                              AAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAGGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCA                                                                                                                       >  SRR19973070.159166/1‑150 (MQ=60)
                                  AGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAA                                                                                                                   >  SRR19973070.2641058/1‑150 (MQ=60)
                                   GAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAAT                                                                                                                  >  SRR19973070.1956432/1‑150 (MQ=60)
                                    AAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATT                                                                                                                 >  SRR19973070.245456/1‑150 (MQ=60)
                                     AACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTA                                                                                                                >  SRR19973070.1895318/1‑150 (MQ=60)
                                       CCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACT                                                                                                              <  SRR19973070.226865/150‑1 (MQ=60)
                                        CGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTT                                                                                                             >  SRR19973070.1092444/1‑150 (MQ=60)
                                         GGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTT                                                                                                            <  SRR19973070.3277157/150‑1 (MQ=60)
                                         GGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTT                                                                                                            >  SRR19973070.1739206/1‑150 (MQ=60)
                                         GGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTT                                                                                                            >  SRR19973070.1739203/1‑150 (MQ=60)
                                         GGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTT                                                                                                            <  SRR19973070.1630662/150‑1 (MQ=60)
                                           TCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGA                                                                                                          <  SRR19973070.1174260/150‑1 (MQ=60)
                                           TCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGA                                                                                                          <  SRR19973070.1174261/150‑1 (MQ=60)
                                           TCAGTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGA                                                                                                          <  SRR19973070.1775138/150‑1 (MQ=60)
                                            CATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGAT                                                                                                         >  SRR19973070.1843082/1‑150 (MQ=60)
                                            CATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGAT                                                                                                         <  SRR19973070.2983604/150‑1 (MQ=60)
                                             ATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATG                                                                                                        >  SRR19973070.139764/1‑150 (MQ=60)
                                             ATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATG                                                                                                        <  SRR19973070.1383503/150‑1 (MQ=60)
                                               TCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTT                                                                                                      <  SRR19973070.64534/150‑1 (MQ=60)
                                                CATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTC                                                                                                     >  SRR19973070.762546/1‑150 (MQ=60)
                                                CATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTC                                                                                                     >  SRR19973070.2376906/1‑150 (MQ=60)
                                                 ATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCC                                                                                                    >  SRR19973070.1557501/1‑150 (MQ=60)
                                                        CAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCAT                                                                                             >  SRR19973070.947548/1‑150 (MQ=60)
                                                        CAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCAT                                                                                             >  SRR19973070.411719/1‑150 (MQ=60)
                                                         AATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATA                                                                                            >  SRR19973070.2465051/1‑150 (MQ=60)
                                                         AATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATA                                                                                            >  SRR19973070.1864070/1‑150 (MQ=60)
                                                         AATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATA                                                                                            >  SRR19973070.1864050/1‑150 (MQ=60)
                                                          ATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAA                                                                                           >  SRR19973070.3121256/1‑150 (MQ=60)
                                                          ATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAA                                                                                           >  SRR19973070.3124665/1‑150 (MQ=60)
                                                               TCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAACTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACC                                                                                      <  SRR19973070.2011896/150‑1 (MQ=60)
                                                                CCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCG                                                                                     <  SRR19973070.3082445/150‑1 (MQ=60)
                                                                CCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCG                                                                                     <  SRR19973070.3082440/150‑1 (MQ=60)
                                                                   CAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGAT                                                                                  <  SRR19973070.1988023/150‑1 (MQ=60)
                                                                    AGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATG                                                                                 <  SRR19973070.798607/150‑1 (MQ=60)
                                                                       CGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACC                                                                              <  SRR19973070.2171750/150‑1 (MQ=60)
                                                                       CGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAACTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACC                                                                              <  SRR19973070.2703692/150‑1 (MQ=60)
                                                                             ATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTACCGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATG                                                                        <  SRR19973070.1304993/150‑1 (MQ=60)
                                                                             ATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATG                                                                        <  SRR19973070.559219/150‑1 (MQ=60)
                                                                             ATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATG                                                                        <  SRR19973070.1303741/150‑1 (MQ=60)
                                                                             ATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATG                                                                        <  SRR19973070.2232579/150‑1 (MQ=60)
                                                                               GACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCA                                                                      <  SRR19973070.2887703/150‑1 (MQ=60)
                                                                               GACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCA                                                                      <  SRR19973070.200758/150‑1 (MQ=60)
                                                                                ACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAG                                                                     <  SRR19973070.828384/150‑1 (MQ=60)
                                                                                    AAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCG                                                                 >  SRR19973070.704346/1‑150 (MQ=60)
                                                                                     AATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGA                                                                >  SRR19973070.1570376/1‑150 (MQ=60)
                                                                                     AATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGA                                                                >  SRR19973070.702973/1‑150 (MQ=60)
                                                                                     AATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGA                                                                >  SRR19973070.2374080/1‑150 (MQ=60)
                                                                                        CGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATC                                                             <  SRR19973070.1576400/150‑1 (MQ=60)
                                                                                         GCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCG                                                            <  SRR19973070.1506051/150‑1 (MQ=60)
                                                                                               AAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTT                                                      >  SRR19973070.2890177/1‑150 (MQ=60)
                                                                                               AAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTT                                                      <  SRR19973070.391227/150‑1 (MQ=60)
                                                                                                AGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTT                                                     >  SRR19973070.809281/1‑150 (MQ=60)
                                                                                                AGCTGAAGGAAAGGAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTT                                                     <  SRR19973070.2183070/150‑1 (MQ=60)
                                                                                                 GCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTT                                                    <  SRR19973070.84413/150‑1 (MQ=60)
                                                                                                   TGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTAC                                                  <  SRR19973070.2272994/150‑1 (MQ=60)
                                                                                                   TGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTAC                                                  >  SRR19973070.2728590/1‑150 (MQ=60)
                                                                                                     AAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACAT                                                <  SRR19973070.473117/150‑1 (MQ=60)
                                                                                                       GGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCC                                              >  SRR19973070.634746/1‑150 (MQ=60)
                                                                                                         AAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCAT                                            >  SRR19973070.2942719/1‑150 (MQ=60)
                                                                                                         AAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATCACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCAT                                            >  SRR19973070.1284503/1‑150 (MQ=60)
                                                                                                              AACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAAT                                       <  SRR19973070.46176/150‑1 (MQ=60)
                                                                                                               ACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATC                                      <  SRR19973070.412654/150‑1 (MQ=60)
                                                                                                               ACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATC                                      <  SRR19973070.2089597/150‑1 (MQ=60)
                                                                                                                  AGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATT                                   <  SRR19973070.2385885/150‑1 (MQ=60)
                                                                                                                    CCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTAC                                 <  SRR19973070.1153987/150‑1 (MQ=60)
                                                                                                                     CGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACA                                >  SRR19973070.1959450/1‑150 (MQ=60)
                                                                                                                       ATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAAC                              <  SRR19973070.1303958/150‑1 (MQ=60)
                                                                                                                       ATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAAC                              >  SRR19973070.794345/1‑150 (MQ=60)
                                                                                                                       ATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAAC                              <  SRR19973070.245660/150‑1 (MQ=60)
                                                                                                                         ACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAG                            <  SRR19973070.29289/150‑1 (MQ=60)
                                                                                                                            CTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTA                         >  SRR19973070.1500126/1‑150 (MQ=60)
                                                                                                                                CTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTG                     >  SRR19973070.3326709/1‑150 (MQ=60)
                                                                                                                                CTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTG                     >  SRR19973070.3326804/1‑150 (MQ=60)
                                                                                                                                CTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTG                     >  SRR19973070.1251727/1‑150 (MQ=60)
                                                                                                                                    CAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAA                 >  SRR19973070.1108565/1‑150 (MQ=60)
                                                                                                                                     AGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAAC                >  SRR19973070.987825/1‑150 (MQ=60)
                                                                                                                                       AACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGT              >  SRR19973070.1999904/1‑150 (MQ=60)
                                                                                                                                       AACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGT              <  SRR19973070.102207/150‑1 (MQ=60)
                                                                                                                                           ATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACA          <  SRR19973070.1006832/150‑1 (MQ=60)
                                                                                                                                              TGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACG       >  SRR19973070.2816060/1‑150 (MQ=60)
                                                                                                                                              TGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACG       >  SRR19973070.216853/1‑150 (MQ=60)
                                                                                                                                                  TTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCAC   <  SRR19973070.3252889/150‑1 (MQ=60)
                                                                                                                                                   TCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACT  <  SRR19973070.300668/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
ACTCCAAATGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACT  >  CP009273/1613940‑1614236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑