Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,614,089 G→T 100% E87* (GAG→TAG)  marA → multiple antibiotic resistance transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,614,0890GT99.0% 680.2 / ‑1.6 209E87* (GAG→TAG) marAmultiple antibiotic resistance transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base T (99/108);  total (99/110)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 209 total aligned reads displayed.

AAATGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAA  >  CP009273/1613945‑1614229
                                                                                                                                                |                                                                                                                                            
  aTGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCg                                                                                                                                         <  2:2751259/148‑1 (MQ=255)
    ggCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGca                                                                                                                                       <  2:2893664/148‑1 (MQ=255)
        ccTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGcaacaa                                                                                                                                   <  1:1060832/148‑1 (MQ=255)
        ccTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGcaacaa                                                                                                                                   <  2:159198/148‑1 (MQ=255)
          tGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAAc                                                                                                                                 >  1:2993335/1‑148 (MQ=255)
            cAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAActc                                                                                                                               <  1:2583971/148‑1 (MQ=255)
            cAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAActc                                                                                                                               <  1:122195/148‑1 (MQ=255)
            cAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAActc                                                                                                                               >  2:1878969/1‑148 (MQ=255)
            cAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAActc                                                                                                                               >  2:3215555/1‑148 (MQ=255)
             aaCGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAActct                                                                                                                              <  1:2145202/148‑1 (MQ=255)
               cGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGa                                                                                                                            >  2:3219721/1‑148 (MQ=255)
                    gTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGa                                                                                                                       >  1:1086978/1‑148 (MQ=255)
                     tttAAAAAAGAAACCGGTCCTTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGaa                                                                                                                      >  1:1391226/1‑148 (MQ=255)
                     tttAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGaa                                                                                                                      <  2:1383921/148‑1 (MQ=255)
                        aaaaaaGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAACAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCt                                                                                                                   <  2:163009/148‑1 (MQ=255)
                               aaaCCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCaaaaa                                                                                                            >  2:535050/1‑148 (MQ=255)
                               aaaCCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCaaaaa                                                                                                            >  2:626261/1‑148 (MQ=255)
                                 aCCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAAtt                                                                                                          >  2:3302345/1‑148 (MQ=255)
                                  ccGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTa                                                                                                         >  2:3113574/1‑148 (MQ=255)
                                   cGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTAc                                                                                                        <  1:1837584/148‑1 (MQ=255)
                                    ggTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACt                                                                                                       <  1:1354478/148‑1 (MQ=255)
                                     gTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACtt                                                                                                      >  1:1408297/1‑148 (MQ=255)
                                       cattcattAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTg                                                                                                    <  2:1458316/148‑1 (MQ=255)
                                       cattcattAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTg                                                                                                    <  1:912916/148‑1 (MQ=255)
                                        attcattAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGa                                                                                                   <  1:1952200/148‑1 (MQ=255)
                                             ttAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTc                                                                                              <  2:1087001/148‑1 (MQ=255)
                                              tAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTcc                                                                                             >  1:842962/1‑148 (MQ=255)
                                                ggCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTccgc                                                                                           >  2:3158151/1‑148 (MQ=255)
                                                ggCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTccgc                                                                                           >  1:1762129/1‑148 (MQ=255)
                                                  ccAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTccgccg                                                                                         <  1:543336/148‑1 (MQ=255)
                                                    aaTACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCa                                                                                       >  2:3340569/1‑148 (MQ=255)
                                                    aaTACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCa                                                                                       >  2:3340168/1‑148 (MQ=255)
                                                    aaTACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCa                                                                                       >  2:2249766/1‑148 (MQ=255)
                                                     aTACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCAt                                                                                      >  2:1718138/1‑148 (MQ=255)
                                                     aTACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCAt                                                                                      >  2:1359593/1‑148 (MQ=255)
                                                      tACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATa                                                                                     >  1:1618149/1‑148 (MQ=255)
                                                         aTCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAAt                                                                                  <  1:3238734/148‑1 (MQ=255)
                                                          tCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATa                                                                                 <  1:1872136/148‑1 (MQ=255)
                                                           ccGCAGCCGTAAGATGACGGAAATCTCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATAc                                                                                >  1:1302602/1‑148 (MQ=255)
                                                           ccGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATAc                                                                                >  2:1412575/1‑148 (MQ=255)
                                                                gCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGAt                                                                           >  2:1688361/1‑148 (MQ=255)
                                                                 ccGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATg                                                                          >  1:309062/1‑148 (MQ=255)
                                                                   gTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGAc                                                                        <  1:795391/148‑1 (MQ=255)
                                                                    tAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGAcc                                                                       >  1:2448318/1‑148 (MQ=255)
                                                                     aaGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCa                                                                      <  2:788034/148‑1 (MQ=255)
                                                                     aaGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCa                                                                      <  1:136160/148‑1 (MQ=255)
                                                                     aaGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCa                                                                      <  2:622546/148‑1 (MQ=255)
                                                                     aaGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCa                                                                      <  1:1675148/148‑1 (MQ=255)
                                                                             ggAAATCGCGCAAACGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAg                                                              <  1:2691368/148‑1 (MQ=255)
                                                                             ggAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAg                                                              <  2:1051368/148‑1 (MQ=255)
                                                                             ggAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAg                                                              <  2:1173998/148‑1 (MQ=255)
                                                                             ggAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAg                                                              <  2:2413629/148‑1 (MQ=255)
                                                                             ggAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAg                                                              <  2:2414080/148‑1 (MQ=255)
                                                                              gAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAgg                                                             >  1:800721/1‑148 (MQ=255)
                                                                              gAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAgg                                                             >  1:360014/1‑148 (MQ=255)
                                                                               aaaTCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAggg                                                            >  1:2463592/1‑148 (MQ=255)
                                                                               aaaTCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAggg                                                            >  1:1791603/1‑148 (MQ=255)
                                                                                  tCGCGCAAAAGCTGAAGTAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGa                                                         >  1:3129368/1‑148 (MQ=255)
                                                                                   cgcgCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGaa                                                        >  2:2174918/1‑148 (MQ=255)
                                                                                   cgcgCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGaa                                                        >  2:2175850/1‑148 (MQ=255)
                                                                                    gcgcAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAAt                                                       <  2:1965524/148‑1 (MQ=255)
                                                                                    gcgcAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAAt                                                       <  2:1351177/148‑1 (MQ=255)
                                                                                     cgcAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATc                                                      >  1:146524/1‑148 (MQ=255)
                                                                                      gcAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATcg                                                     <  2:1142341/148‑1 (MQ=255)
                                                                                        aaaaGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATcgcg                                                   >  1:1999710/1‑148 (MQ=255)
                                                                                         aaaGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCgcgc                                                  <  2:2643840/148‑1 (MQ=255)
                                                                                         aaaGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCgcgc                                                  >  1:691506/1‑148 (MQ=255)
                                                                                          aaGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCt                                                 <  1:1878969/148‑1 (MQ=255)
                                                                                          aaGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCt                                                 >  2:1360415/1‑148 (MQ=255)
                                                                                          aaGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCt                                                 <  1:3215563/148‑1 (MQ=255)
                                                                                           aGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACACACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATCCAGGGCGAATCGCGCtt                                                <  2:1833713/148‑1 (MQ=255)
                                                                                            gCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCttt                                               <  2:2287793/148‑1 (MQ=255)
                                                                                            gCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCttt                                               <  2:1494808/148‑1 (MQ=255)
                                                                                             cTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCtttt                                              <  1:2184220/148‑1 (MQ=255)
                                                                                                aaGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTAc                                           <  2:1803594/148‑1 (MQ=255)
                                                                                                aaGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTAc                                           <  1:3311308/148‑1 (MQ=255)
                                                                                                   gAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATc                                        <  1:1417169/148‑1 (MQ=255)
                                                                                                   gAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATc                                        <  1:2827842/148‑1 (MQ=255)
                                                                                                     aaGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCa                                      <  1:644044/148‑1 (MQ=255)
                                                                                                      aGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCAt                                     <  2:1246343/148‑1 (MQ=255)
                                                                                                         aaCGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTaa                                  <  2:1240066/148‑1 (MQ=255)
                                                                                                         aaCGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTaa                                  <  1:2672582/148‑1 (MQ=255)
                                                                                                          aCGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTaaa                                 >  1:1701443/1‑148 (MQ=255)
                                                                                                            gAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATc                               >  1:1095243/1‑148 (MQ=255)
                                                                                                               ccGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCAtt                            <  2:2015138/148‑1 (MQ=255)
                                                                                                                cGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTa                           <  1:535050/148‑1 (MQ=255)
                                                                                                                cGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTa                           >  1:2788536/1‑148 (MQ=255)
                                                                                                                  aTACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTaca                         <  2:1360749/148‑1 (MQ=255)
                                                                                                                       ctATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGc                    >  2:2092259/1‑148 (MQ=255)
                                                                                                                       ctATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGc                    <  1:17202/148‑1 (MQ=255)
                                                                                                                             ggCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTg              >  1:2018210/1‑148 (MQ=255)
                                                                                                                                 gAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGaaaa          <  1:3403142/148‑1 (MQ=255)
                                                                                                                                     gATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTg      <  2:1757984/148‑1 (MQ=255)
                                                                                                                                      atatGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGa     >  1:432577/1‑148 (MQ=255)
                                                                                                                                       tatGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGAc    >  2:1881382/1‑148 (MQ=255)
                                                                                                                                       tatGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGAc    >  2:1881253/1‑148 (MQ=255)
                                                                                                                                        atGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACa   <  2:2576375/148‑1 (MQ=255)
                                                                                                                                        atGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACa   <  2:1482367/148‑1 (MQ=255)
                                                                                                                                        atGGCTTCTAGTCGCAACAAAATCTGACCCGAACCTTCAAAAATTACTTTTATGTACCGCCGCATAAATAACTGATGACCAATAAGCAGGGCGAATCGCGCTTTATACATCCATTAAATCATTACAACAGCTATTTGAAAACGAGACa   >  1:2574940/1‑148 (MQ=255)
                                                                                                                                         tGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACaa  >  1:2655453/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                            
AAATGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAA  >  CP009273/1613945‑1614229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCAAATGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGA  >  CP009273/1613943‑1614238
                                                                                                                                                  |                                                                                                                                                     
CCAAATGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGT                                                                                                                                                    >  SRR19973065.2028126/1‑150 (MQ=60)
 CAAATGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTC                                                                                                                                                   >  SRR19973065.87875/1‑150 (MQ=60)
 CAAATGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTC                                                                                                                                                   >  SRR19973065.1491830/1‑150 (MQ=60)
   AATGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGC                                                                                                                                                 >  SRR19973065.547059/1‑150 (MQ=60)
     TGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAA                                                                                                                                               >  SRR19973065.998602/1‑150 (MQ=60)
          CCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAAC                                                                                                                                          <  SRR19973065.1067864/150‑1 (MQ=60)
          CCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAAC                                                                                                                                          >  SRR19973065.3011665/1‑150 (MQ=60)
            TGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTC                                                                                                                                        >  SRR19973065.1978353/1‑150 (MQ=60)
              CAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTG                                                                                                                                      <  SRR19973065.123261/150‑1 (MQ=60)
              CAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTG                                                                                                                                      <  SRR19973065.2600071/150‑1 (MQ=60)
              CAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTG                                                                                                                                      <  SRR19973065.2833303/150‑1 (MQ=60)
              CAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTG                                                                                                                                      <  SRR19973065.2953565/150‑1 (MQ=60)
               AACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGA                                                                                                                                     <  SRR19973065.2158998/150‑1 (MQ=60)
                 CGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACC                                                                                                                                   >  SRR19973065.3252174/1‑150 (MQ=60)
                  GGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCC                                                                                                                                  >  SRR19973065.1119740/1‑150 (MQ=60)
                    ATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGA                                                                                                                                >  SRR19973065.1094145/1‑150 (MQ=60)
                    ATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGA                                                                                                                                >  SRR19973065.1094168/1‑150 (MQ=60)
                     TGTTTAAAAAAGAAACCGGTCCTTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAA                                                                                                                               >  SRR19973065.1400359/1‑150 (MQ=60)
                       TTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACC                                                                                                                             <  SRR19973065.2138307/150‑1 (MQ=60)
                         TAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTT                                                                                                                           <  SRR19973065.1912095/150‑1 (MQ=60)
                         TAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTT                                                                                                                           <  SRR19973065.129171/150‑1 (MQ=60)
                          AAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTC                                                                                                                          <  SRR19973065.3412080/150‑1 (MQ=60)
                                     CGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTT                                                                                                               >  SRR19973065.1417512/1‑150 (MQ=60)
                                     CGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTT                                                                                                               <  SRR19973065.1849702/150‑1 (MQ=60)
                                      GGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTT                                                                                                              <  SRR19973065.1363453/150‑1 (MQ=60)
                                         CATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGAT                                                                                                           <  SRR19973065.2665257/150‑1 (MQ=60)
                                         CATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGAT                                                                                                           <  SRR19973065.918864/150‑1 (MQ=60)
                                          ATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATG                                                                                                          <  SRR19973065.387839/150‑1 (MQ=60)
                                          ATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATG                                                                                                          <  SRR19973065.1964958/150‑1 (MQ=60)
                                              ATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCC                                                                                                      >  SRR19973065.848439/1‑150 (MQ=60)
                                                TAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGC                                                                                                    >  SRR19973065.1773694/1‑150 (MQ=60)
                                                 AGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCC                                                                                                   <  SRR19973065.2164703/150‑1 (MQ=60)
                                                    CCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCA                                                                                                <  SRR19973065.546855/150‑1 (MQ=60)
                                                     CAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCAT                                                                                               <  SRR19973065.2478681/150‑1 (MQ=60)
                                                      AATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATA                                                                                              >  SRR19973065.1629004/1‑150 (MQ=60)
                                                           ATCCGCAGCCGTAAGATGACGGAAATCTCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTATGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATAC                                                                                         >  SRR19973065.1311179/1‑150 (MQ=60)
                                                           ATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATAC                                                                                         <  SRR19973065.3258238/150‑1 (MQ=60)
                                                            TCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACC                                                                                        <  SRR19973065.1884465/150‑1 (MQ=60)
                                                            TCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACC                                                                                        <  SRR19973065.1884878/150‑1 (MQ=60)
                                                                 AGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATG                                                                                   >  SRR19973065.311136/1‑150 (MQ=60)
                                                                    CGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACC                                                                                >  SRR19973065.836856/1‑150 (MQ=60)
                                                                    CGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACC                                                                                >  SRR19973065.2463746/1‑150 (MQ=60)
                                                                     GTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCA                                                                               >  SRR19973065.1882009/1‑150 (MQ=60)
                                                                     GTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCA                                                                               <  SRR19973065.800664/150‑1 (MQ=60)
                                                                     GTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCA                                                                               <  SRR19973065.800651/150‑1 (MQ=60)
                                                                       AAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAAT                                                                             >  SRR19973065.1075480/1‑150 (MQ=60)
                                                                       AAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAAT                                                                             <  SRR19973065.137325/150‑1 (MQ=60)
                                                                       AAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAAT                                                                             <  SRR19973065.1686356/150‑1 (MQ=60)
                                                                             ACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAG                                                                       >  SRR19973065.746246/1‑150 (MQ=60)
                                                                              CGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGG                                                                      >  SRR19973065.362544/1‑150 (MQ=60)
                                                                              CGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGG                                                                      >  SRR19973065.1372398/1‑150 (MQ=60)
                                                                              CGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGG                                                                      >  SRR19973065.806015/1‑150 (MQ=60)
                                                                               GGAAATCGCGCAAACGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGG                                                                     <  SRR19973065.2708080/150‑1 (MQ=60)
                                                                               GGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGG                                                                     >  SRR19973065.1803352/1‑150 (MQ=60)
                                                                               GGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGG                                                                     >  SRR19973065.2479120/1‑150 (MQ=60)
                                                                                 AAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCG                                                                   >  SRR19973065.165172/1‑150 (MQ=60)
                                                                                  AATCGCGCAAAAGCTGAAGTAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGA                                                                  >  SRR19973065.3148413/1‑150 (MQ=60)
                                                                                     CGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATC                                                               <  SRR19973065.1407308/150‑1 (MQ=60)
                                                                                     CGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATC                                                               <  SRR19973065.3011849/150‑1 (MQ=60)
                                                                                     CGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATC                                                               <  SRR19973065.1466512/150‑1 (MQ=60)
                                                                                     CGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATC                                                               >  SRR19973065.147764/1‑150 (MQ=60)
                                                                                        GCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCG                                                            >  SRR19973065.2012655/1‑150 (MQ=60)
                                                                                         CAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGC                                                           >  SRR19973065.696113/1‑150 (MQ=60)
                                                                                            AAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTT                                                        <  SRR19973065.1891340/150‑1 (MQ=60)
                                                                                            AAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTT                                                        <  SRR19973065.3234902/150‑1 (MQ=60)
                                                                                            AAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTT                                                        <  SRR19973065.3234893/150‑1 (MQ=60)
                                                                                             AGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTT                                                       >  SRR19973065.1776452/1‑150 (MQ=60)
                                                                                               CTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTA                                                     <  SRR19973065.1803829/150‑1 (MQ=60)
                                                                                               CTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTA                                                     <  SRR19973065.2198234/150‑1 (MQ=60)
                                                                                                  AAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACAT                                                  <  SRR19973065.3331477/150‑1 (MQ=60)
                                                                                                  AAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACAT                                                  <  SRR19973065.3331489/150‑1 (MQ=60)
                                                                                                    GGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCC                                                <  SRR19973065.695482/150‑1 (MQ=60)
                                                                                                     GAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCA                                               <  SRR19973065.1426424/150‑1 (MQ=60)
                                                                                                     GAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCA                                               <  SRR19973065.2845292/150‑1 (MQ=60)
                                                                                                       AAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATT                                             <  SRR19973065.2350466/150‑1 (MQ=60)
                                                                                                       AAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATT                                             <  SRR19973065.648270/150‑1 (MQ=60)
                                                                                                        AGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTA                                            <  SRR19973065.2914484/150‑1 (MQ=60)
                                                                                                          TAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAA                                          >  SRR19973065.1712577/1‑150 (MQ=60)
                                                                                                          TAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAA                                          >  SRR19973065.1712783/1‑150 (MQ=60)
                                                                                                           AACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAAT                                         <  SRR19973065.2689144/150‑1 (MQ=60)
                                                                                                           AACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAAT                                         <  SRR19973065.2687988/150‑1 (MQ=60)
                                                                                                            ACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATC                                        >  SRR19973065.1102451/1‑150 (MQ=60)
                                                                                                              gcccccagacgcgaccgccCAGAACGATCGGGCTTCTAGCCGCAACACACTCTGACCCGAACCTTCAAAAATTACTTGGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCAT                                      <  SRR19973065.1912204/131‑1 (MQ=60)
                                                                                                               AGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATT                                     <  SRR19973065.1361744/150‑1 (MQ=60)
                                                                                                               AGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATT                                     <  SRR19973065.3117722/150‑1 (MQ=60)
                                                                                                                GCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTA                                    >  SRR19973065.2805822/1‑150 (MQ=60)
                                                                                                                  CGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACA                                  >  SRR19973065.360197/1‑150 (MQ=60)
                                                                                                                  CGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACA                                  <  SRR19973065.538539/150‑1 (MQ=60)
                                                                                                                   GATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAA                                 >  SRR19973065.2592443/1‑150 (MQ=60)
                                                                                                                      ACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAG                              <  SRR19973065.606339/150‑1 (MQ=60)
                                                                                                                       CTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGC                             >  SRR19973065.2041818/1‑150 (MQ=60)
                                                                                                                         CTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTA                           <  SRR19973065.1421806/150‑1 (MQ=60)
                                                                                                                         CTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTA                           <  SRR19973065.17446/150‑1 (MQ=60)
                                                                                                                           ATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGT                         <  SRR19973065.2707665/150‑1 (MQ=60)
                                                                                                                           ATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGT                         <  SRR19973065.2708155/150‑1 (MQ=60)
                                                                                                                            TCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTT                        <  SRR19973065.543328/150‑1 (MQ=60)
                                                                                                                             CTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTG                       >  SRR19973065.2031205/1‑150 (MQ=60)
                                                                                                                                   GAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACG                 <  SRR19973065.3423939/150‑1 (MQ=60)
                                                                                                                                    AACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGT                <  SRR19973065.1729559/150‑1 (MQ=60)
                                                                                                                                    AACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGT                >  SRR19973065.1639584/1‑150 (MQ=60)
                                                                                                                                     ACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTG               <  SRR19973065.1369419/150‑1 (MQ=60)
                                                                                                                                      CGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCTCATTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGA              >  SRR19973065.1744674/1‑150 (MQ=60)
                                                                                                                                      CGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGA              >  SRR19973065.435583/1‑150 (MQ=60)
                                                                                                                                      CGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGA              >  SRR19973065.435582/1‑150 (MQ=60)
                                                                                                                                        ATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACA            >  SRR19973065.2590988/1‑150 (MQ=60)
                                                                                                                                        ATATGGCTTCTAGTCGCAACAAAATCTGACCCGAACCTTCAAAAATTACTTTTATGTACCGCCGCATAAATAACTGATGACCAATAAGCAGGGCGAATCGCGCTTTATACATCCATTAAATCATTACAACAGCTATTTGAAAACGAGACA            >  SRR19973065.2591003/1‑150 (MQ=60)
                                                                                                                                         TATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAA           >  SRR19973065.2671863/1‑150 (MQ=60)
                                                                                                                                                  TAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGA  >  SRR19973065.3315088/1‑150 (MQ=60)
                                                                                                                                                  TAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGA  >  SRR19973065.3315085/1‑150 (MQ=60)
                                                                                                                                                  |                                                                                                                                                     
CCAAATGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGA  >  CP009273/1613943‑1614238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑