Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,614,089 G→T 100% E87* (GAG→TAG)  marA → multiple antibiotic resistance transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,614,0890GT98.4% 777.9 / 2.9 244E87* (GAG→TAG) marAmultiple antibiotic resistance transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base T (123/117);  total (125/119)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 244 total aligned reads displayed.

AAATGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTC  >  CP009273/1613945‑1614233
                                                                                                                                                |                                                                                                                                                
aaaTGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGt                                                                                                                                               >  1:2325928/1‑148 (MQ=255)
aaaTGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGt                                                                                                                                               <  2:3257491/148‑1 (MQ=255)
aaaTGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGt                                                                                                                                               >  1:401415/1‑148 (MQ=255)
   tGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGc                                                                                                                                            >  1:1896552/1‑148 (MQ=255)
        ccTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGcaacaa                                                                                                                                       <  2:3251381/148‑1 (MQ=255)
           gCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAAct                                                                                                                                    <  2:178567/148‑1 (MQ=255)
           gCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAAct                                                                                                                                    <  2:1889441/148‑1 (MQ=255)
              aCGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTg                                                                                                                                 <  2:2464238/148‑1 (MQ=255)
                 gATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGAcc                                                                                                                              >  2:1499751/1‑148 (MQ=255)
                  aTGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGAccc                                                                                                                             >  1:816945/1‑148 (MQ=255)
                  aTGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGAccc                                                                                                                             >  1:598650/1‑148 (MQ=255)
                    gTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGa                                                                                                                           >  1:3453206/1‑148 (MQ=255)
                    gTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGa                                                                                                                           >  2:1179815/1‑148 (MQ=255)
                    gTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGa                                                                                                                           <  2:3812787/148‑1 (MQ=255)
                          aaaaGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTc                                                                                                                     >  2:1080894/1‑148 (MQ=255)
                           aaaGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCa                                                                                                                    >  1:3037854/1‑148 (MQ=255)
                           aaaGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCa                                                                                                                    >  1:1590216/1‑148 (MQ=255)
                             aGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCaaa                                                                                                                  >  1:2053865/1‑148 (MQ=255)
                              gAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTACCGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCaaaa                                                                                                                 <  1:41302/148‑1 (MQ=255)
                               aaaCCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCaaaaa                                                                                                                >  2:262824/1‑148 (MQ=255)
                                  ccGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTa                                                                                                             <  2:514604/148‑1 (MQ=255)
                                    ggTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACt                                                                                                           <  1:3639960/148‑1 (MQ=255)
                                    ggTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACt                                                                                                           <  1:1189517/148‑1 (MQ=255)
                                    ggTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACt                                                                                                           <  1:706137/148‑1 (MQ=255)
                                    ggTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACt                                                                                                           <  2:2140843/148‑1 (MQ=255)
                                     gTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACtt                                                                                                          >  2:1579882/1‑148 (MQ=255)
                                     gTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACtt                                                                                                          >  1:986225/1‑148 (MQ=255)
                                     gTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACtt                                                                                                          <  2:102728/148‑1 (MQ=255)
                                     gTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACtt                                                                                                          >  2:1641807/1‑148 (MQ=255)
                                     gTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACtt                                                                                                          <  2:573219/148‑1 (MQ=255)
                                      tcattcatTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACttt                                                                                                         >  1:890875/1‑148 (MQ=255)
                                      tcattcatTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACttt                                                                                                         >  2:1678711/1‑148 (MQ=255)
                                       cattcattAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTg                                                                                                        <  2:1162886/148‑1 (MQ=255)
                                       cattcattAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTg                                                                                                        >  1:1038675/1‑148 (MQ=255)
                                       cattcattAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTg                                                                                                        >  1:2614426/1‑148 (MQ=255)
                                       cattcattAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTg                                                                                                        >  1:2832616/1‑148 (MQ=255)
                                         ttcattAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGAt                                                                                                      >  1:370469/1‑148 (MQ=255)
                                          tcattAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATg                                                                                                     <  2:1975503/148‑1 (MQ=255)
                                          tcattAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACACTGACCCGAACCTTCAAAAATTACTTTCATg                                                                                                     >  1:2316050/1‑148 (MQ=255)
                                                 gCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTccgcc                                                                                              >  1:3540242/1‑148 (MQ=255)
                                                     aTACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCAt                                                                                          >  1:504849/1‑148 (MQ=255)
                                                     aTACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCAt                                                                                          >  1:971228/1‑148 (MQ=255)
                                                       aCATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATaa                                                                                        <  1:2366165/148‑1 (MQ=255)
                                                       aCATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATaa                                                                                        >  2:1604378/1‑148 (MQ=255)
                                                       aCATCCGCAGCCGTAAGATGACAGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATaa                                                                                        >  2:3648418/1‑148 (MQ=255)
                                                          tCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATa                                                                                     <  2:2403712/148‑1 (MQ=255)
                                                           ccGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATAc                                                                                    <  1:1390221/148‑1 (MQ=255)
                                                           ccGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATAc                                                                                    >  2:2048130/1‑148 (MQ=255)
                                                           ccGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATAc                                                                                    <  1:1389763/148‑1 (MQ=255)
                                                            cGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATAcc                                                                                   <  1:3122132/148‑1 (MQ=255)
                                                               aGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGa                                                                                <  1:1635206/148‑1 (MQ=255)
                                                                gCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGAt                                                                               >  2:1828993/1‑148 (MQ=255)
                                                                      aGATGACTGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCaa                                                                         <  2:2616785/148‑1 (MQ=255)
                                                                      aGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCaa                                                                         >  1:2232759/1‑148 (MQ=255)
                                                                      aGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCaa                                                                         >  2:2432429/1‑148 (MQ=255)
                                                                      aGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCaa                                                                         <  2:2578755/148‑1 (MQ=255)
                                                                        aTGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAata                                                                       <  2:3143032/148‑1 (MQ=255)
                                                                             ggAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAg                                                                  >  2:220236/1‑148 (MQ=255)
                                                                             ggAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAg                                                                  <  1:3371583/148‑1 (MQ=255)
                                                                             ggAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAg                                                                  <  2:3508328/148‑1 (MQ=255)
                                                                               aaaTCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAggg                                                                <  1:2518054/148‑1 (MQ=255)
                                                                               aaaTCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAggg                                                                <  2:2753139/148‑1 (MQ=255)
                                                                                 aTCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCg                                                              >  2:295913/1‑148 (MQ=255)
                                                                                  tCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGa                                                             <  1:1693633/148‑1 (MQ=255)
                                                                                    gcgcAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAAt                                                           <  2:22346/148‑1 (MQ=255)
                                                                                      gcAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATcg                                                         <  1:2750564/148‑1 (MQ=255)
                                                                                        aaaaGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACAGGATGACAAATATGCAGGGCGAATcgcg                                                       >  1:2268386/1‑148 (MQ=255)
                                                                                         aaaGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCgcgc                                                      >  2:3021922/1‑148 (MQ=255)
                                                                                          aaGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCt                                                     >  1:3244283/1‑148 (MQ=255)
                                                                                           aGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCtt                                                    <  1:17156/148‑1 (MQ=255)
                                                                                           aGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCtt                                                    >  2:472169/1‑148 (MQ=255)
                                                                                           aGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCtt                                                    <  2:370469/148‑1 (MQ=255)
                                                                                             cTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCtttt                                                  <  2:2762269/148‑1 (MQ=255)
                                                                                               gAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTa                                                >  1:2708894/1‑148 (MQ=255)
                                                                                               gAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTa                                                >  1:2621655/1‑148 (MQ=255)
                                                                                                aatgattgTAACGAGCCGATACTCTTTCTGTCAGATCTTTATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCTAAAATTACTTTGATGTTACGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATAGCGCTTTTTAc                                               <  2:2849805/141‑1 (MQ=255)
                                                                                                aaGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTAc                                               <  2:1005816/148‑1 (MQ=255)
                                                                                                  ggAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCTCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGTACTACCAAAATGCAGGGCGAATCGCGCTTTTTACAt                                             >  2:2128125/1‑148 (MQ=255)
                                                                                                  ggAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACAt                                             >  1:624898/1‑148 (MQ=255)
                                                                                                    aaaGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATcc                                           <  2:910007/148‑1 (MQ=255)
                                                                                                        tAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTa                                       >  1:2821431/1‑148 (MQ=255)
                                                                                                            gAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATc                                   <  1:3260298/148‑1 (MQ=255)
                                                                                                              gCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCAt                                 >  2:938486/1‑148 (MQ=255)
                                                                                                                 gATACTCTATCTGGCAGAACGATATGGCGTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTac                              >  2:3639702/1‑148 (MQ=255)
                                                                                                                  aTACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTaca                             <  2:1851682/148‑1 (MQ=255)
                                                                                                                  aTACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTaca                             >  1:2006367/1‑148 (MQ=255)
                                                                                                                   tACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTacaa                            <  2:394263/148‑1 (MQ=255)
                                                                                                                   tACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTacaa                            <  1:286153/148‑1 (MQ=255)
                                                                                                                     ctctATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTacaaca                          >  1:3411892/1‑148 (MQ=255)
                                                                                                                      tctATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAg                         >  1:172427/1‑148 (MQ=255)
                                                                                                                      tctATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAg                         >  1:1861900/1‑148 (MQ=255)
                                                                                                                       ctATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGc                        >  2:3031854/1‑148 (MQ=255)
                                                                                                                          tCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAg                     >  1:2332273/1‑148 (MQ=255)
                                                                                                                                 gAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGaaaa              <  1:1027196/148‑1 (MQ=255)
                                                                                                                                 gAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGaaaa              <  2:226596/148‑1 (MQ=255)
                                                                                                                                  aaCGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAAc             >  2:3559913/1‑148 (MQ=255)
                                                                                                                                       tatGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGAc        >  2:709454/1‑148 (MQ=255)
                                                                                                                                        atGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACa       >  1:2202463/1‑148 (MQ=255)
                                                                                                                                        atGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACa       <  1:2749635/148‑1 (MQ=255)
                                                                                                                                             ttCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTc  <  1:2451027/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
AAATGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTC  >  CP009273/1613945‑1614233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTCCAAATGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCAC  >  CP009273/1613941‑1614235
                                                                                                                                                    |                                                                                                                                                  
CTCCAAATGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTA                                                                                                                                                   >  SRR19973069.978942/1‑150 (MQ=60)
  CCAAATGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGT                                                                                                                                                 >  SRR19973069.2341747/1‑150 (MQ=60)
  CCAAATGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGT                                                                                                                                                 >  SRR19973069.2341844/1‑150 (MQ=60)
  CCAAATGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGT                                                                                                                                                 >  SRR19973069.2341856/1‑150 (MQ=60)
  CCAAATGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGT                                                                                                                                                 >  SRR19973069.404348/1‑150 (MQ=60)
  CCAAATGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGT                                                                                                                                                 >  SRR19973069.404352/1‑150 (MQ=60)
  CCAAATGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGT                                                                                                                                                 >  SRR19973069.404367/1‑150 (MQ=60)
     AATGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGC                                                                                                                                              >  SRR19973069.1909682/1‑150 (MQ=60)
         GCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACA                                                                                                                                          >  SRR19973069.2034847/1‑150 (MQ=60)
            CCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAAC                                                                                                                                       >  SRR19973069.3656987/1‑150 (MQ=60)
             CTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACT                                                                                                                                      <  SRR19973069.2877460/150‑1 (MQ=60)
                CAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTG                                                                                                                                   <  SRR19973069.1159861/150‑1 (MQ=60)
                    GGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCC                                                                                                                               >  SRR19973069.822713/1‑150 (MQ=60)
                    GGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCC                                                                                                                               >  SRR19973069.602689/1‑150 (MQ=60)
                      ATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGA                                                                                                                             >  SRR19973069.3475590/1‑150 (MQ=60)
                        GTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAAC                                                                                                                           <  SRR19973069.996361/150‑1 (MQ=60)
                             AAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCA                                                                                                                      >  SRR19973069.1600975/1‑150 (MQ=60)
                             AAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCA                                                                                                                      >  SRR19973069.1600996/1‑150 (MQ=60)
                             AAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCA                                                                                                                      >  SRR19973069.3057988/1‑150 (MQ=60)
                               AAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAA                                                                                                                    >  SRR19973069.2068117/1‑150 (MQ=60)
                                 AGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAA                                                                                                                  >  SRR19973069.2069678/1‑150 (MQ=60)
                                 AGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAA                                                                                                                  >  SRR19973069.3775742/1‑150 (MQ=60)
                                  GAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTACCGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAAT                                                                                                                 <  SRR19973069.41840/150‑1 (MQ=60)
                                       CGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTT                                                                                                            >  SRR19973069.992895/1‑150 (MQ=60)
                                       CGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTT                                                                                                            >  SRR19973069.992904/1‑150 (MQ=60)
                                        GGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTT                                                                                                           >  SRR19973069.618807/1‑150 (MQ=60)
                                        GGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTT                                                                                                           <  SRR19973069.711095/150‑1 (MQ=60)
                                        GGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTT                                                                                                           >  SRR19973069.897022/1‑150 (MQ=60)
                                        GGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTT                                                                                                           <  SRR19973069.1197725/150‑1 (MQ=60)
                                        GGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTT                                                                                                           <  SRR19973069.3663808/150‑1 (MQ=60)
                                         GTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTG                                                                                                          >  SRR19973069.2851324/1‑150 (MQ=60)
                                         GTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTG                                                                                                          >  SRR19973069.2631903/1‑150 (MQ=60)
                                         GTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTG                                                                                                          >  SRR19973069.2631559/1‑150 (MQ=60)
                                         GTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTG                                                                                                          >  SRR19973069.1045752/1‑150 (MQ=60)
                                         GTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTG                                                                                                          >  SRR19973069.1045891/1‑150 (MQ=60)
                                           CATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGAT                                                                                                        >  SRR19973069.373170/1‑150 (MQ=60)
                                           CATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGAT                                                                                                        >  SRR19973069.373064/1‑150 (MQ=60)
                                           CATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGCAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGAT                                                                                                        <  SRR19973069.332840/150‑1 (MQ=60)
                                            ATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATG                                                                                                       >  SRR19973069.226554/1‑150 (MQ=60)
                                            ATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATG                                                                                                       <  SRR19973069.875954/150‑1 (MQ=60)
                                            ATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATG                                                                                                       >  SRR19973069.2332036/1‑150 (MQ=60)
                                            ATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACACTGACCCGAACCTTCAAAAATTACTTTCATG                                                                                                       >  SRR19973069.2331814/1‑150 (MQ=60)
                                                  TAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGC                                                                                                 >  SRR19973069.2673557/1‑150 (MQ=60)
                                                   AGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCC                                                                                                >  SRR19973069.3563208/1‑150 (MQ=60)
                                                   AGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCC                                                                                                >  SRR19973069.311911/1‑150 (MQ=60)
                                                       CAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCAT                                                                                            >  SRR19973069.2848878/1‑150 (MQ=60)
                                                       CAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCAT                                                                                            >  SRR19973069.977716/1‑150 (MQ=60)
                                                       CAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCAT                                                                                            >  SRR19973069.508460/1‑150 (MQ=60)
                                                        AATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATA                                                                                           >  SRR19973069.2633131/1‑150 (MQ=60)
                                                         ATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAA                                                                                          >  SRR19973069.2245257/1‑150 (MQ=60)
                                                           ACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAAT                                                                                        <  SRR19973069.3460024/150‑1 (MQ=60)
                                                           ACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAAT                                                                                        <  SRR19973069.2382235/150‑1 (MQ=60)
                                                               CCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCG                                                                                    <  SRR19973069.1399917/150‑1 (MQ=60)
                                                               CCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCG                                                                                    <  SRR19973069.1399458/150‑1 (MQ=60)
                                                               CCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCG                                                                                    <  SRR19973069.1399455/150‑1 (MQ=60)
                                                                CGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGG                                                                                   <  SRR19973069.3144552/150‑1 (MQ=60)
                                                                CGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGG                                                                                   <  SRR19973069.3142641/150‑1 (MQ=60)
                                                                  CAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGAT                                                                                 <  SRR19973069.879040/150‑1 (MQ=60)
                                                                  CAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGAT                                                                                 <  SRR19973069.689465/150‑1 (MQ=60)
                                                                   AGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATG                                                                                <  SRR19973069.1646401/150‑1 (MQ=60)
                                                                    GCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAAAGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGA                                                                               <  SRR19973069.1735551/150‑1 (MQ=60)
                                                                      CGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACC                                                                             >  SRR19973069.1650048/1‑150 (MQ=60)
                                                                        TAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAA                                                                           >  SRR19973069.2247834/1‑150 (MQ=60)
                                                                         AAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAAT                                                                          <  SRR19973069.3547409/150‑1 (MQ=60)
                                                                          AGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATA                                                                         >  SRR19973069.3470120/1‑150 (MQ=60)
                                                                           GATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATAT                                                                        >  SRR19973069.578888/1‑150 (MQ=60)
                                                                            ATGACGGATATCGCGCAAAAGCTGATTGAAAGTAACGCTCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTTCTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATG                                                                       <  SRR19973069.2998372/150‑1 (MQ=60)
                                                                             TGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGC                                                                      >  SRR19973069.9398/1‑150 (MQ=60)
                                                                             TGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGC                                                                      >  SRR19973069.193308/1‑150 (MQ=60)
                                                                                CGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGG                                                                   <  SRR19973069.2425765/150‑1 (MQ=60)
                                                                                 GGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGG                                                                  <  SRR19973069.3393626/150‑1 (MQ=60)
                                                                                   AAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCG                                                                <  SRR19973069.2534723/150‑1 (MQ=60)
                                                                                   AAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCG                                                                >  SRR19973069.1802805/1‑150 (MQ=60)
                                                                                   AAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCG                                                                <  SRR19973069.2534805/150‑1 (MQ=60)
                                                                                      TCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAAT                                                             <  SRR19973069.1705468/150‑1 (MQ=60)
                                                                                       CGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATC                                                            <  SRR19973069.496949/150‑1 (MQ=60)
                                                                                       CGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACCCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATC                                                            <  SRR19973069.1912228/150‑1 (MQ=60)
                                                                                        GCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCG                                                           <  SRR19973069.1242794/150‑1 (MQ=60)
                                                                                          GCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCG                                                         >  SRR19973069.2287582/1‑150 (MQ=60)
                                                                                          GCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCG                                                         <  SRR19973069.2768933/150‑1 (MQ=60)
                                                                                          GCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCG                                                         <  SRR19973069.227247/150‑1 (MQ=60)
                                                                                          GCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACAGGATGACAAATATGCAGGGCGAATCGCG                                                         >  SRR19973069.2283758/1‑150 (MQ=60)
                                                                                           CAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGC                                                        >  SRR19973069.3072921/1‑150 (MQ=60)
                                                                                            ANAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCT                                                       >  SRR19973069.3265519/1‑150 (MQ=60)
                                                                                            AAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCT                                                       >  SRR19973069.3266093/1‑150 (MQ=60)
                                                                                              AAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTT                                                     >  SRR19973069.3583538/1‑150 (MQ=60)
                                                                                               AGCTGAAGGAACGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTT                                                    <  SRR19973069.17374/150‑1 (MQ=60)
                                                                                               AGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTT                                                    >  SRR19973069.3410534/1‑150 (MQ=60)
                                                                                               AGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTT                                                    <  SRR19973069.18802/150‑1 (MQ=60)
                                                                                               AGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTT                                                    <  SRR19973069.17398/150‑1 (MQ=60)
                                                                                                 CTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTA                                                  >  SRR19973069.2727056/1‑150 (MQ=60)
                                                                                                 CTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTA                                                  >  SRR19973069.3368833/1‑150 (MQ=60)
                                                                                                 CTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTA                                                  >  SRR19973069.2639184/1‑150 (MQ=60)
                                                                                                    AAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACAT                                               >  SRR19973069.629047/1‑150 (MQ=60)
                                                                                                      GGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCC                                             >  SRR19973069.2366514/1‑150 (MQ=60)
                                                                                                        aaacaAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCAT                                           <  SRR19973069.2453713/145‑1 (MQ=60)
                                                                                                          AGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCAGTA                                         >  SRR19973069.2840098/1‑150 (MQ=60)
                                                                                                                GAGCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCAT                                   <  SRR19973069.3281653/150‑1 (MQ=60)
                                                                                                                  GCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTA                                 <  SRR19973069.2798808/150‑1 (MQ=60)
                                                                                                                  GCCGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTA                                 <  SRR19973069.2799346/150‑1 (MQ=60)
                                                                                                                    CGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACA                               <  SRR19973069.1272295/150‑1 (MQ=60)
                                                                                                                    CGATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACA                               >  SRR19973069.2020333/1‑150 (MQ=60)
                                                                                                                      ATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAAC                             <  SRR19973069.2589464/150‑1 (MQ=60)
                                                                                                                      ATACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAAC                             <  SRR19973069.648366/150‑1 (MQ=60)
                                                                                                                       TACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACA                            <  SRR19973069.1682373/150‑1 (MQ=60)
                                                                                                                       TACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACA                            <  SRR19973069.287973/150‑1 (MQ=60)
                                                                                                                       TACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACA                            <  SRR19973069.288130/150‑1 (MQ=60)
                                                                                                                       TACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACA                            >  SRR19973069.3434124/1‑150 (MQ=60)
                                                                                                                        ACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAG                           >  SRR19973069.1902991/1‑150 (MQ=60)
                                                                                                                        ACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAG                           >  SRR19973069.173892/1‑150 (MQ=60)
                                                                                                                        ACTCTATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAG                           >  SRR19973069.1874891/1‑150 (MQ=60)
                                                                                                                            TATCTGGCAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAG                       >  SRR19973069.2348150/1‑150 (MQ=60)
                                                                                                                                   CAGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAA                <  SRR19973069.1446365/150‑1 (MQ=60)
                                                                                                                                    AGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAAC               <  SRR19973069.264715/150‑1 (MQ=60)
                                                                                                                                    AGAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAAC               >  SRR19973069.822380/1‑150 (MQ=60)
                                                                                                                                     GAACGATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACG              <  SRR19973069.1034311/150‑1 (MQ=60)
                                                                                                                                          ATATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACA         >  SRR19973069.2217411/1‑150 (MQ=60)
                                                                                                                                           TATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAA        >  SRR19973069.2096651/1‑150 (MQ=60)
                                                                                                                                            ATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAAC       <  SRR19973069.1510147/150‑1 (MQ=60)
                                                                                                                                            ATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAAC       <  SRR19973069.2768190/150‑1 (MQ=60)
                                                                                                                                            ATGGCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAAC       <  SRR19973069.2768000/150‑1 (MQ=60)
                                                                                                                                               GCTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTC    <  SRR19973069.2763278/150‑1 (MQ=60)
                                                                                                                                                CTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCA   >  SRR19973069.1543059/1‑150 (MQ=60)
                                                                                                                                                CTTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCA   <  SRR19973069.984315/150‑1 (MQ=60)
                                                                                                                                                 TTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCAC  <  SRR19973069.535400/150‑1 (MQ=60)
                                                                                                                                                 TTCTAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCAC  <  SRR19973069.2467512/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                  
CTCCAAATGGCACCTGCAACGGATGTTTAAAAAAGAAACCGGTCATTCATTAGGCCAATACATCCGCAGCCGTAAGATGACGGAAATCGCGCAAAAGCTGAAGGAAAGTAACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCAC  >  CP009273/1613941‑1614235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑