Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,614,837 G→T 44.7% I186I (ATC→ATA)  eamA ← cysteine and O‑acetyl‑L‑serine efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,614,8370GT44.7% 158.8 / 225.0 190I186I (ATC→ATA) eamAcysteine and O‑acetyl‑L‑serine efflux system
Reads supporting (aligned to +/- strand):  ref base G (43/62);  new base T (44/41);  total (87/103)
Fisher's exact test for biased strand distribution p-value = 1.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 193 total aligned reads displayed.

TAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCACATGCT  >  CP009273/1614690‑1614982
                                                                                                                                                   |                                                                                                                                                 
tAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATg                                                                                                                                                   <  2:1336582/148‑1 (MQ=255)
  aCGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTAt                                                                                                                                                 <  2:3123198/148‑1 (MQ=255)
   cGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAattatt                                                                                                                                                >  1:1785877/1‑148 (MQ=255)
   cGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGAtt                                                                                                                                                >  1:2811524/1‑148 (MQ=255)
    gTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTg                                                                                                                                               <  1:1160373/148‑1 (MQ=255)
      cccccAGATCCCATAACCTACTATTGTCGCAACAAACGCCAGATACATCAGAGACAAGATGGTGTTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTggg                                                                                                                                             <  1:1688289/148‑1 (MQ=255)
       ccccAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGa                                                                                                                                            <  2:279071/148‑1 (MQ=255)
           aGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTaa                                                                                                                                        >  1:443824/1‑148 (MQ=255)
            gATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTaaa                                                                                                                                       <  2:838625/148‑1 (MQ=255)
               cccATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAgcg                                                                                                                                    <  1:1803367/148‑1 (MQ=255)
                  aTAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTc                                                                                                                                 >  1:3101911/1‑148 (MQ=255)
                  aTAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTc                                                                                                                                 >  1:1978938/1‑148 (MQ=255)
                   tAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTcc                                                                                                                                >  1:707976/1‑148 (MQ=255)
                      ccAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGa                                                                                                                             >  2:2453300/1‑148 (MQ=255)
                      ccAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGa                                                                                                                             >  1:1606743/1‑148 (MQ=255)
                      ccAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGa                                                                                                                             >  2:2103370/1‑148 (MQ=255)
                         acaaTTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTa                                                                                                                          <  2:319818/148‑1 (MQ=255)
                         acaaTTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTa                                                                                                                          >  1:1037119/1‑148 (MQ=255)
                           aaTTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTAcc                                                                                                                        <  2:2566838/148‑1 (MQ=255)
                           aaTTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTAcc                                                                                                                        <  2:3308099/148‑1 (MQ=255)
                               gTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCg                                                                                                                    <  2:333561/148‑1 (MQ=255)
                                   ccACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACAt                                                                                                                >  1:1672221/1‑148 (MQ=255)
                                      caAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCAc                                                                                                             >  1:2654521/1‑148 (MQ=255)
                                        aaCGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCAccg                                                                                                           <  2:1996564/148‑1 (MQ=255)
                                         aCGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCAccgc                                                                                                          <  1:2959269/148‑1 (MQ=255)
                                         aCGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCAccgc                                                                                                          >  2:1564550/1‑148 (MQ=255)
                                             cAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGa                                                                                                      <  2:2927114/148‑1 (MQ=255)
                                             cAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGa                                                                                                      <  2:3074950/148‑1 (MQ=255)
                                               gATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACACAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGAcg                                                                                                    >  1:405764/1‑148 (MQ=255)
                                                   cATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGtt                                                                                                >  1:3276797/1‑148 (MQ=255)
                                                       agagACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGt                                                                                            <  1:2329139/148‑1 (MQ=255)
                                                         agaCAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGc                                                                                          <  2:429079/148‑1 (MQ=255)
                                                          gaCAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCg                                                                                         >  1:994319/1‑148 (MQ=255)
                                                          gaCAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCg                                                                                         >  1:994261/1‑148 (MQ=255)
                                                          gaCAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCg                                                                                         <  1:1038507/148‑1 (MQ=255)
                                                                aTGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGa                                                                                   <  2:2154961/148‑1 (MQ=255)
                                                                aTGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGa                                                                                   >  2:2716017/1‑148 (MQ=255)
                                                                   gtggtCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCt                                                                                <  2:523199/148‑1 (MQ=255)
                                                                   gtggtCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCt                                                                                >  2:1219124/1‑148 (MQ=255)
                                                                       tCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCttttt                                                                            <  2:326187/148‑1 (MQ=255)
                                                                       tCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCttttt                                                                            >  1:1026012/1‑148 (MQ=255)
                                                                        cATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTa                                                                           <  1:1255599/148‑1 (MQ=255)
                                                                           atCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTg                                                                        >  2:980938/1‑148 (MQ=255)
                                                                           atCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTg                                                                        >  1:1348594/1‑148 (MQ=255)
                                                                           atCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTg                                                                        >  2:2377600/1‑148 (MQ=255)
                                                                           atCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTg                                                                        <  1:757019/148‑1 (MQ=255)
                                                                           atCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTg                                                                        <  1:2415869/148‑1 (MQ=255)
                                                                           atCGATAGTAACCAGAATTTGAATCATGGTTGCGGAACCATCGAGAATCACCGAGTCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACTCGTTGAGTGCGACTTGATCTTTTTATTg                                                                        <  1:1099220/148‑1 (MQ=255)
                                                                            tCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGa                                                                       <  2:2812134/148‑1 (MQ=255)
                                                                              gATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAg                                                                     <  2:1754026/148‑1 (MQ=255)
                                                                                tAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGACTGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTGTATTGAAGAt                                                                   >  2:1965329/1‑148 (MQ=255)
                                                                                tAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGAt                                                                   >  2:1554031/1‑148 (MQ=255)
                                                                                tAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGAt                                                                   >  2:617936/1‑148 (MQ=255)
                                                                                 aGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATg                                                                  <  1:3199584/148‑1 (MQ=255)
                                                                                 aGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATg                                                                  <  1:1163949/148‑1 (MQ=255)
                                                                                  gTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGt                                                                 <  2:3143716/148‑1 (MQ=255)
                                                                                   tAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGtt                                                                >  2:1920406/1‑148 (MQ=255)
                                                                                     aCCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGc                                                              >  1:276422/1‑148 (MQ=255)
                                                                                     aCCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGc                                                              >  1:2912822/1‑148 (MQ=255)
                                                                                     aCCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGtttc                                                              <  1:3037781/148‑3 (MQ=255)
                                                                                        aGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCcac                                                           <  2:1435116/148‑1 (MQ=255)
                                                                                                  tCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACt                                                 >  1:2639617/1‑148 (MQ=255)
                                                                                                    aTGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTaa                                               >  1:1983588/1‑148 (MQ=255)
                                                                                                    aTGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTaa                                               <  1:3311310/148‑1 (MQ=255)
                                                                                                       gTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAAtg                                            <  1:1522296/148‑1 (MQ=255)
                                                                                                       gTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAAtg                                            <  2:2173512/148‑1 (MQ=255)
                                                                                                          gCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAAtgctg                                         <  1:980938/148‑1 (MQ=255)
                                                                                                          gCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAAtgctg                                         <  2:1979692/148‑1 (MQ=255)
                                                                                                           cGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAAtgctgc                                        <  1:2462328/148‑1 (MQ=255)
                                                                                                             gAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTgccg                                      >  1:3173597/1‑148 (MQ=255)
                                                                                                             gAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTgccg                                      >  1:3173286/1‑148 (MQ=255)
                                                                                                              aaCCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTgccgc                                     <  1:2183348/148‑1 (MQ=255)
                                                                                                              aaCCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTgccgc                                     <  1:2183441/148‑1 (MQ=255)
                                                                                                               aCCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTgccgcc                                    <  2:2004980/148‑1 (MQ=255)
                                                                                                               aCCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTgccgcc                                    <  2:2338677/148‑1 (MQ=255)
                                                                                                                ccATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCa                                   >  1:1788184/1‑148 (MQ=255)
                                                                                                                ccATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCa                                   >  1:2743119/1‑148 (MQ=255)
                                                                                                                ccATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCa                                   <  2:108483/148‑1 (MQ=255)
                                                                                                                  aTCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAgg                                 >  2:405933/1‑148 (MQ=255)
                                                                                                                        aaTCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAAc                           >  2:3001483/1‑148 (MQ=255)
                                                                                                                          tCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACAt                         >  1:77497/1‑148 (MQ=255)
                                                                                                                          tCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACAt                         >  1:2128291/1‑148 (MQ=255)
                                                                                                                             gCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATaaa                      <  1:2511013/148‑1 (MQ=255)
                                                                                                                             gCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATaaa                      <  1:2510909/148‑1 (MQ=255)
                                                                                                                             gCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATaaa                      >  1:2537731/1‑148 (MQ=255)
                                                                                                                              cGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAg                     <  1:2153772/148‑1 (MQ=255)
                                                                                                                              cGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAg                     <  1:2077854/148‑1 (MQ=255)
                                                                                                                              cGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAg                     <  2:1708050/148‑1 (MQ=255)
                                                                                                                              cGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAg                     <  2:1787221/148‑1 (MQ=255)
                                                                                                                                   caacaaAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAg                <  1:1918745/148‑1 (MQ=255)
                                                                                                                                     acaaAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCa              <  1:2925377/148‑1 (MQ=255)
                                                                                                                                      caaAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCAt             <  2:1882602/148‑1 (MQ=255)
                                                                                                                                         agaagGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGc          >  1:1563778/1‑148 (MQ=255)
                                                                                                                                           aagGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCca        >  2:2873902/1‑148 (MQ=255)
                                                                                                                                           aagGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCca        >  2:1296305/1‑148 (MQ=255)
                                                                                                                                              ggAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCACAt     >  2:450751/1‑148 (MQ=255)
                                                                                                                                               gAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCACATg    >  2:1250809/1‑148 (MQ=255)
                                                                                                                                                aaTTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCACATGc   >  1:66004/1‑148 (MQ=255)
                                                                                                                                                aaTGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCACATGc   >  2:1176871/1‑148 (MQ=255)
                                                                                                                                                 aTGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCACATGCt  >  1:1377715/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCACATGCT  >  CP009273/1614690‑1614982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCACATGCT  >  CP009273/1614689‑1614982
                                                                                                                                                    |                                                                                                                                                 
GTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTA                                                                                                                                                  <  SRR19973070.1087152/150‑1 (MQ=60)
GTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTA                                                                                                                                                  <  SRR19973070.1087128/150‑1 (MQ=60)
 TAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTAT                                                                                                                                                 >  SRR19973070.526751/1‑150 (MQ=60)
  AACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATT                                                                                                                                                >  SRR19973070.1798226/1‑150 (MQ=60)
  AACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATT                                                                                                                                                >  SRR19973070.2829703/1‑150 (MQ=60)
  AACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATT                                                                                                                                                >  SRR19973070.2830318/1‑150 (MQ=60)
     GTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGG                                                                                                                                             <  SRR19973070.1168325/150‑1 (MQ=60)
     GTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGG                                                                                                                                             <  SRR19973070.1168490/150‑1 (MQ=60)
       CCCCCAGATCCCATAACCTACTATTGTCGCAACAAACGCCAGATACATCAGAGACAAGATGGTGTTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGAT                                                                                                                                           <  SRR19973070.1699887/150‑1 (MQ=60)
       CCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGAT                                                                                                                                           <  SRR19973070.1358501/150‑1 (MQ=60)
          CCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAA                                                                                                                                        >  SRR19973070.447033/1‑150 (MQ=60)
             GATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGACTTATTGGGATTAAAGC                                                                                                                                     >  SRR19973070.3004443/1‑150 (MQ=60)
                CCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCT                                                                                                                                  <  SRR19973070.1815815/150‑1 (MQ=60)
                 CCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTC                                                                                                                                 >  SRR19973070.1993082/1‑150 (MQ=60)
                 CCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTC                                                                                                                                 >  SRR19973070.1992317/1‑150 (MQ=60)
                 CCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTC                                                                                                                                 >  SRR19973070.3121912/1‑150 (MQ=60)
                  CATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCC                                                                                                                                >  SRR19973070.2384781/1‑150 (MQ=60)
                  CATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCC                                                                                                                                >  SRR19973070.683763/1‑150 (MQ=60)
                  CATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCC                                                                                                                                >  SRR19973070.713028/1‑150 (MQ=60)
                   ATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCA                                                                                                                               >  SRR19973070.2662787/1‑150 (MQ=60)
                   ATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCA                                                                                                                               <  SRR19973070.1420682/150‑1 (MQ=60)
                     AACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCTGCGAGGCAACAAAGAAGGGAATTATTGGGAGTAAAGCGCTCCAGA                                                                                                                             >  SRR19973070.1617946/1‑150 (MQ=60)
                       CCAACAATTGCCCCCACAAAAGCCAGATAAATCAGAGACAAGATGTTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGTAACCATCGAGAATCAGCGAGTCAACTAAGAAGCGAATGATTGGGATTAAAGCGCTCCAGATT                                                                                                                           <  SRR19973070.29657/150‑1 (MQ=60)
                        CAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTA                                                                                                                          >  SRR19973070.1044447/1‑150 (MQ=60)
                           CAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGACGGGAATTATTGGGATTAAAGCGCTCCAGATTACCA                                                                                                                       >  SRR19973070.3272403/1‑150 (MQ=60)
                                  CGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACAT                                                                                                                >  SRR19973070.1683685/1‑150 (MQ=60)
                                     CACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCAC                                                                                                             >  SRR19973070.2671790/1‑150 (MQ=60)
                                     CACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCAC                                                                                                             >  SRR19973070.2050499/1‑150 (MQ=60)
                                         AACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCC                                                                                                         <  SRR19973070.1842120/150‑1 (MQ=60)
                                          ACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCG                                                                                                        <  SRR19973070.2978303/150‑1 (MQ=60)
                                              CAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACACAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACG                                                                                                    >  SRR19973070.408741/1‑150 (MQ=60)
                                              CAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACG                                                                                                    >  SRR19973070.408758/1‑150 (MQ=60)
                                                  TACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTT                                                                                                >  SRR19973070.3297854/1‑150 (MQ=60)
                                                   ACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTG                                                                                               <  SRR19973070.1621424/150‑1 (MQ=60)
                                                       CAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTG                                                                                           >  SRR19973070.318443/1‑150 (MQ=60)
                                                        AGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGC                                                                                          <  SRR19973070.2344612/150‑1 (MQ=60)
                                                         GAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCG                                                                                         >  SRR19973070.1001401/1‑150 (MQ=60)
                                                         GAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCG                                                                                         >  SRR19973070.1001350/1‑150 (MQ=60)
                                                         GAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCG                                                                                         >  SRR19973070.1001408/1‑150 (MQ=60)
                                                           GACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGAC                                                                                       <  SRR19973070.1045850/150‑1 (MQ=60)
                                                           GACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGAC                                                                                       <  SRR19973070.1045841/150‑1 (MQ=60)
                                                           GACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGAC                                                                                       <  SRR19973070.1045828/150‑1 (MQ=60)
                                                                 ATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATC                                                                                 <  SRR19973070.748477/150‑1 (MQ=60)
                                                                  TGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCT                                                                                <  SRR19973070.3320389/150‑1 (MQ=60)
                                                                  TGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCT                                                                                <  SRR19973070.2282398/150‑1 (MQ=60)
                                                                   GGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTT                                                                               <  SRR19973070.1591900/150‑1 (MQ=60)
                                                                      GGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTT                                                                            >  SRR19973070.1033287/1‑150 (MQ=60)
                                                                         CATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATT                                                                         <  SRR19973070.1264405/150‑1 (MQ=60)
                                                                          ATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTG                                                                        >  SRR19973070.1357840/1‑150 (MQ=60)
                                                                          ATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTG                                                                        >  SRR19973070.1357703/1‑150 (MQ=60)
                                                                            ATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAA                                                                      <  SRR19973070.2431771/150‑1 (MQ=60)
                                                                            ATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAA                                                                      <  SRR19973070.762355/150‑1 (MQ=60)
                                                                            ATCGATAGTAACCAGAATTTGAATCATGGTTGCGGAACCATCGAGAATCACCGAGTCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACTCGTTGAGTGCGACTTGATCTTTTTATTGAA                                                                      <  SRR19973070.1106780/150‑1 (MQ=60)
                                                                               GATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGAT                                                                   <  SRR19973070.2469344/150‑1 (MQ=60)
                                                                               GATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGAT                                                                   <  SRR19973070.2939045/150‑1 (MQ=60)
                                                                               GATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGAT                                                                   >  SRR19973070.2175266/1‑150 (MQ=60)
                                                                                ATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATG                                                                  <  SRR19973070.2778135/150‑1 (MQ=60)
                                                                                  AGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTT                                                                <  SRR19973070.770764/150‑1 (MQ=60)
                                                                                  AGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTT                                                                <  SRR19973070.1172098/150‑1 (MQ=60)
                                                                                  AGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTT                                                                <  SRR19973070.1171276/150‑1 (MQ=60)
                                                                                  AGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTT                                                                <  SRR19973070.3220288/150‑1 (MQ=60)
                                                                                    TAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGC                                                              >  SRR19973070.278215/1‑150 (MQ=60)
                                                                                    TAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGC                                                              >  SRR19973070.2931647/1‑150 (MQ=60)
                                                                                     AACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCC                                                             <  SRR19973070.1686945/150‑1 (MQ=60)
                                                                                      ACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTTCCA                                                            <  SRR19973070.3057098/150‑1 (MQ=60)
                                                                                                GAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAAC                                                  >  SRR19973070.2789425/1‑150 (MQ=60)
                                                                                                 AATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACT                                                 >  SRR19973070.2656819/1‑150 (MQ=60)
                                                                                                   TCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAA                                               >  SRR19973070.1997015/1‑150 (MQ=60)
                                                                                                     ATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAAT                                             <  SRR19973070.3332490/150‑1 (MQ=60)
                                                                                                     ATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAAT                                             <  SRR19973070.3332498/150‑1 (MQ=60)
                                                                                                       GGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGC                                           >  SRR19973070.2482080/1‑150 (MQ=60)
                                                                                                        GTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCT                                          <  SRR19973070.1532982/150‑1 (MQ=60)
                                                                                                         TTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTG                                         <  SRR19973070.1791614/150‑1 (MQ=60)
                                                                                                           GCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCC                                       <  SRR19973070.987935/150‑1 (MQ=60)
                                                                                                           GCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCC                                       <  SRR19973070.987961/150‑1 (MQ=60)
                                                                                                            CGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCG                                      <  SRR19973070.1566113/150‑1 (MQ=60)
                                                                                                            CGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCG                                      <  SRR19973070.2478405/150‑1 (MQ=60)
                                                                                                            CGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCG                                      >  SRR19973070.3194141/1‑150 (MQ=60)
                                                                                                            CGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCG                                      >  SRR19973070.3193826/1‑150 (MQ=60)
                                                                                                            CGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCG                                      >  SRR19973070.3193896/1‑150 (MQ=60)
                                                                                                            CGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCG                                      >  SRR19973070.3091187/1‑150 (MQ=60)
                                                                                                             GGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGC                                     >  SRR19973070.1976452/1‑150 (MQ=60)
                                                                                                               AACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCA                                   >  SRR19973070.1800545/1‑150 (MQ=60)
                                                                                                               AACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCA                                   <  SRR19973070.2197790/150‑1 (MQ=60)
                                                                                                               AACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCA                                   <  SRR19973070.2197821/150‑1 (MQ=60)
                                                                                                               AACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCA                                   <  SRR19973070.2197883/150‑1 (MQ=60)
                                                                                                               AACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCA                                   >  SRR19973070.2760761/1‑150 (MQ=60)
                                                                                                                ACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAG                                  >  SRR19973070.905298/1‑150 (MQ=60)
                                                                                                                  CATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGG                                <  SRR19973070.258323/150‑1 (MQ=60)
                                                                                                                         AATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACAT                         >  SRR19973070.78320/1‑150 (MQ=60)
                                                                                                                         AATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACAT                         >  SRR19973070.2142591/1‑150 (MQ=60)
                                                                                                                         AATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACAT                         >  SRR19973070.2142527/1‑150 (MQ=60)
                                                                                                                            CAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAA                      >  SRR19973070.2554319/1‑150 (MQ=60)
                                                                                                                            CAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAA                      <  SRR19973070.1806148/150‑1 (MQ=60)
                                                                                                                            CAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGAGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAA                      >  SRR19973070.2128964/1‑150 (MQ=60)
                                                                                                                              GCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGC                    <  SRR19973070.2531679/150‑1 (MQ=60)
                                                                                                                              GCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGC                    <  SRR19973070.2527359/150‑1 (MQ=60)
                                                                                                                              GCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGC                    <  SRR19973070.2527254/150‑1 (MQ=60)
                                                                                                                               CGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCC                   <  SRR19973070.2168105/150‑1 (MQ=60)
                                                                                                                               CGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCC                   <  SRR19973070.2091840/150‑1 (MQ=60)
                                                                                                                                  GGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAG                >  SRR19973070.1210048/1‑150 (MQ=60)
                                                                                                                                    CAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCA              <  SRR19973070.1931685/150‑1 (MQ=60)
                                                                                                                                      ACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATC            <  SRR19973070.2736643/150‑1 (MQ=60)
                                                                                                                                      ACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATC            <  SRR19973070.2944264/150‑1 (MQ=60)
                                                                                                                                        AAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGC          >  SRR19973070.1574786/1‑150 (MQ=60)
                                                                                                                                               GGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCACATGC   >  SRR19973070.66737/1‑150 (MQ=60)
                                                                                                                                                GAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCACATGCT  >  SRR19973070.1387064/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                 
GTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCACATGCT  >  CP009273/1614689‑1614982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑