Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,614,837 G→T 29.8% I186I (ATC→ATA)  eamA ← cysteine and O‑acetyl‑L‑serine efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,614,8370GT29.8% 408.3 / 151.5 211I186I (ATC→ATA) eamAcysteine and O‑acetyl‑L‑serine efflux system
Reads supporting (aligned to +/- strand):  ref base G (89/59);  new base T (31/32);  total (120/91)
Fisher's exact test for biased strand distribution p-value = 1.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 212 total aligned reads displayed.

AACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCACATGCTGA  >  CP009273/1614691‑1614984
                                                                                                                                                  |                                                                                                                                                   
aaCGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGACTGa                                                                                                                                                    >  2:1367190/1‑148 (MQ=255)
  cGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACCAAGAAGGGAATGAtt                                                                                                                                                  >  2:2771806/1‑148 (MQ=255)
    tCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTgg                                                                                                                                                >  2:194997/1‑148 (MQ=255)
    tCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTgg                                                                                                                                                <  2:2874790/148‑1 (MQ=255)
     cccccAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTggg                                                                                                                                               <  2:2331280/148‑1 (MQ=255)
     cccccAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTggg                                                                                                                                               >  1:3482684/1‑148 (MQ=255)
          aGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTaa                                                                                                                                          <  1:685230/148‑1 (MQ=255)
            aTCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGCTTAAAg                                                                                                                                        >  2:2932756/1‑148 (MQ=255)
             tCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAgc                                                                                                                                       >  1:823072/1‑148 (MQ=255)
              cccATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAgcg                                                                                                                                      >  1:1690721/1‑148 (MQ=255)
               ccATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAgcgc                                                                                                                                     <  2:3041139/148‑1 (MQ=255)
                 aTAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTc                                                                                                                                   >  1:1003980/1‑148 (MQ=255)
                 aTAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTc                                                                                                                                   >  1:2779682/1‑148 (MQ=255)
                  tAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTcc                                                                                                                                  >  2:1853377/1‑148 (MQ=255)
                    aCCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAg                                                                                                                                <  1:1052512/148‑1 (MQ=255)
                     ccAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGa                                                                                                                               <  2:743907/148‑1 (MQ=255)
                     ccAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGa                                                                                                                               >  2:2819204/1‑148 (MQ=255)
                      caacacTTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGAt                                                                                                                              <  1:1885211/148‑1 (MQ=255)
                      caacaaTTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGAt                                                                                                                              <  1:2646624/148‑1 (MQ=255)
                        acaaTTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTa                                                                                                                            >  2:3015902/1‑148 (MQ=255)
                             tGTCGCCACAAACGCTAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGTCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGc                                                                                                                       >  1:700213/1‑148 (MQ=255)
                                   cacaAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATc                                                                                                                 <  2:803339/148‑1 (MQ=255)
                                     caAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCAc                                                                                                               <  1:4308/148‑1 (MQ=255)
                                        aCGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCAccgc                                                                                                            <  2:3285486/148‑1 (MQ=255)
                                           ccAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCgg                                                                                                         >  1:1049981/1‑148 (MQ=255)
                                               aTACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGACTGATTGGGATTCAAGCGCTCCAGATTACCAGCGACATCACCGCCGGAcgc                                                                                                     >  2:2720448/1‑148 (MQ=255)
                                                tACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGAcgcg                                                                                                    <  2:3451679/148‑1 (MQ=255)
                                                tACATCAGAGACAAGATGGTGGTCATATAGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTATAGCGCTCCAGATTACCAGCGACATCACCGCCGGAcgcg                                                                                                    <  2:1690721/148‑1 (MQ=255)
                                                  cATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGtt                                                                                                  <  1:164094/148‑1 (MQ=255)
                                                  cATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGtt                                                                                                  <  1:345546/148‑1 (MQ=255)
                                                    tCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGa                                                                                                >  1:2497983/1‑148 (MQ=255)
                                                    tCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGa                                                                                                <  2:2585590/148‑1 (MQ=255)
                                                      agagACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGt                                                                                              <  1:2830983/148‑1 (MQ=255)
                                                          aCAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGa                                                                                          >  2:903949/1‑148 (MQ=255)
                                                          aCAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGa                                                                                          <  1:3507/148‑1 (MQ=255)
                                                           cAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGGTTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGAc                                                                                         <  1:733186/148‑1 (MQ=255)
                                                           cAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGAc                                                                                         <  1:3048277/148‑1 (MQ=255)
                                                           cAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGAc                                                                                         >  1:1836724/1‑148 (MQ=255)
                                                             aGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACAt                                                                                       >  2:1089446/1‑148 (MQ=255)
                                                                 ggtggtCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATc                                                                                   >  1:1083596/1‑148 (MQ=255)
                                                                  gtggtCATATCGATAGTAACCAGACTGTGACTCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCt                                                                                  <  2:2440859/148‑1 (MQ=255)
                                                                  gtggtCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCt                                                                                  <  1:236629/148‑1 (MQ=255)
                                                                   tggtCATATCGATAGTAACCAGACTGTGAATCAGGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCtt                                                                                 <  2:3148399/148‑1 (MQ=255)
                                                                     gtCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCtttt                                                                               >  1:315949/1‑148 (MQ=255)
                                                                     gtCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCtttt                                                                               <  2:115584/148‑1 (MQ=255)
                                                                          atCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTg                                                                          >  2:1789051/1‑148 (MQ=255)
                                                                           tCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTCAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGa                                                                         >  2:2028203/1‑148 (MQ=255)
                                                                            cGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGaa                                                                        >  2:357563/1‑148 (MQ=255)
                                                                               tAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGAt                                                                     <  2:3482684/148‑1 (MQ=255)
                                                                                aGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATg                                                                    <  1:2406714/148‑1 (MQ=255)
                                                                                 gTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGt                                                                   <  1:1173165/148‑1 (MQ=255)
                                                                                  tAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGtt                                                                  >  2:1547954/1‑148 (MQ=255)
                                                                                  tAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGtt                                                                  >  1:1558658/1‑148 (MQ=255)
                                                                                   aaCCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTg                                                                 <  1:2729392/148‑1 (MQ=255)
                                                                                   aaCCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTg                                                                 <  1:1597347/148‑1 (MQ=255)
                                                                                     ccAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGcc                                                               >  2:3510606/1‑148 (MQ=255)
                                                                                     ccAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGcc                                                               >  2:1208968/1‑148 (MQ=255)
                                                                                     ccAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGcc                                                               >  2:775917/1‑148 (MQ=255)
                                                                                     ccAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGcc                                                               >  2:1739796/1‑148 (MQ=255)
                                                                                      cAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCca                                                              >  1:417806/1‑148 (MQ=255)
                                                                                            gtgAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGcc                                                        <  1:1332669/148‑1 (MQ=255)
                                                                                              gAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCa                                                      >  1:331979/1‑148 (MQ=255)
                                                                                              gAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCa                                                      <  1:2593703/148‑1 (MQ=255)
                                                                                               aaTCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCaa                                                     <  2:797080/148‑1 (MQ=255)
                                                                                               aaTCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCaa                                                     <  2:2394582/148‑1 (MQ=255)
                                                                                               aaTCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCaa                                                     >  1:3394279/1‑148 (MQ=255)
                                                                                                aTCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAAc                                                    >  2:522277/1‑148 (MQ=255)
                                                                                                aTCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAAc                                                    <  2:2669606/148‑1 (MQ=255)
                                                                                                      gTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAAtg                                              <  1:2219580/148‑1 (MQ=255)
                                                                                                         gCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAAtgctg                                           <  1:2073676/148‑1 (MQ=255)
                                                                                                         gCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAAtgctg                                           <  1:789444/148‑1 (MQ=255)
                                                                                                          cGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAAtgctgc                                          <  2:2888288/148‑1 (MQ=255)
                                                                                                            gAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTgccg                                        >  1:136741/1‑148 (MQ=255)
                                                                                                             aaCCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTgccgc                                       >  2:1586428/1‑148 (MQ=255)
                                                                                                             aaCCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTgccgc                                       <  1:434983/148‑1 (MQ=255)
                                                                                                                 aTCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAgg                                   <  1:2525603/148‑1 (MQ=255)
                                                                                                                   cGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGt                                 >  2:1826932/1‑148 (MQ=255)
                                                                                                                   cGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGt                                 >  2:429153/1‑148 (MQ=255)
                                                                                                                   cGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGt                                 >  2:2220997/1‑148 (MQ=255)
                                                                                                                     agaATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCa                               <  1:903949/148‑1 (MQ=255)
                                                                                                                     agaATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCa                               >  2:1809666/1‑148 (MQ=255)
                                                                                                                     agaATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCa                               >  2:2179225/1‑148 (MQ=255)
                                                                                                                      gaATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCaa                              >  1:3261968/1‑148 (MQ=255)
                                                                                                                       aaTCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAAc                             >  1:2270876/1‑148 (MQ=255)
                                                                                                                       aaTCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAAc                             >  1:2261923/1‑148 (MQ=255)
                                                                                                                            gCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATaaa                        >  1:2988960/1‑148 (MQ=255)
                                                                                                                                ggCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCg                    >  1:1490648/1‑148 (MQ=255)
                                                                                                                                     caaAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACTCGCCCAACTAAATGCTGCCGCCAGGGTCGACATAAAGCCGAGCAt               >  2:2257950/1‑148 (MQ=255)
                                                                                                                                     caaAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCAt               >  2:1300350/1‑148 (MQ=255)
                                                                                                                                     caaAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCAt               >  2:3072223/1‑148 (MQ=255)
                                                                                                                                         gaagGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGcc           >  1:2003713/1‑148 (MQ=255)
                                                                                                                                         gaagGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGcc           >  1:875255/1‑148 (MQ=255)
                                                                                                                                            gggAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCcaca        <  1:386806/148‑1 (MQ=255)
                                                                                                                                             ggAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCACAt       <  2:349792/148‑1 (MQ=255)
                                                                                                                                              gAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCACATg      <  2:823072/148‑1 (MQ=255)
                                                                                                                                              gAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCACATg      <  1:2456551/148‑1 (MQ=255)
                                                                                                                                               aaTTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCACATGc     >  2:732734/1‑148 (MQ=255)
                                                                                                                                               aaTTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCACATGc     >  2:1380267/1‑148 (MQ=255)
                                                                                                                                               aaTTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCACATGc     <  1:2383733/148‑1 (MQ=255)
                                                                                                                                               aaTGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCACATGc     >  1:591700/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
AACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCACATGCTGA  >  CP009273/1614691‑1614984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A