Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,614,837 G→T 100% I186I (ATC→ATA)  eamA ← cysteine and O‑acetyl‑L‑serine efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,614,8370GT97.6% 634.9 / 6.0 205I186I (ATC→ATA) eamAcysteine and O‑acetyl‑L‑serine efflux system
Reads supporting (aligned to +/- strand):  ref base G (3/2);  new base T (105/95);  total (108/97)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 205 total aligned reads displayed.

ACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCACATGC  >  CP009273/1614692‑1614981
                                                                                                                                                 |                                                                                                                                                
aCGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTAt                                                                                                                                                <  2:827153/148‑1 (MQ=255)
aCGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTAt                                                                                                                                                <  1:90716/148‑1 (MQ=255)
  gTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTg                                                                                                                                              <  1:272084/148‑1 (MQ=255)
    cccccAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGACTTATTggg                                                                                                                                            >  2:2522121/1‑148 (MQ=255)
    cccccAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTggg                                                                                                                                            <  2:3292581/148‑1 (MQ=255)
    cccccAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATAAATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTggg                                                                                                                                            <  2:426478/148‑1 (MQ=255)
     ccccAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGa                                                                                                                                           >  2:74733/1‑148 (MQ=255)
        cAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAATGAAGGGAATTATTGGGATTa                                                                                                                                        >  2:3375841/1‑148 (MQ=255)
        cAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTa                                                                                                                                        >  1:793717/1‑148 (MQ=255)
         aGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTaa                                                                                                                                       >  2:2540304/1‑148 (MQ=255)
          gATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTaaa                                                                                                                                      <  2:266332/148‑1 (MQ=255)
           aTCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGACTTATTGGGATTAAAg                                                                                                                                     >  1:1547980/1‑148 (MQ=255)
            tCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAgc                                                                                                                                    <  2:810483/148‑1 (MQ=255)
               cATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGACTTATTGGGATTAAAGCGCt                                                                                                                                 >  2:212729/1‑148 (MQ=255)
                 tAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAAGTATTGGGATTAAAGCGCTcc                                                                                                                               >  2:3378024/1‑148 (MQ=255)
                   aCCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAg                                                                                                                             >  1:2168964/1‑148 (MQ=255)
                    ccAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAATGAAGGGAATTATTGGGATTAAAGCGCTCCAGa                                                                                                                            >  2:2462837/1‑148 (MQ=255)
                    ccAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGa                                                                                                                            <  2:2714168/148‑1 (MQ=255)
                    ccAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGa                                                                                                                            >  1:3305102/1‑148 (MQ=255)
                       acaaTTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTa                                                                                                                         >  1:2436038/1‑148 (MQ=255)
                        caaTTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGAAACAAAGCAGGGAATTATTGGGATTAAAGAGCTCCAGATTAc                                                                                                                        >  2:2100192/1‑148 (MQ=255)
                         aaTTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTAcc                                                                                                                       <  1:1715103/148‑1 (MQ=255)
                         aaTTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTAcc                                                                                                                       >  2:1249106/1‑148 (MQ=255)
                                   acaAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCATAATGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTTGGATTAAAGCGATCCAGATTACCAGCGACATCa                                                                                                             <  2:1962118/148‑1 (MQ=255)
                                        cGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCAccgcc                                                                                                        >  1:3240534/1‑148 (MQ=255)
                                               tACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGAcgcg                                                                                                 <  2:162490/148‑1 (MQ=255)
                                                aCATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGt                                                                                                <  2:2173074/148‑1 (MQ=255)
                                                   tCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGa                                                                                             >  1:532922/1‑148 (MQ=255)
                                                     agagACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGt                                                                                           <  1:2951929/148‑1 (MQ=255)
                                                      gagaCAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTg                                                                                          <  1:2646535/148‑1 (MQ=255)
                                                       agaCAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGc                                                                                         >  1:1172517/1‑148 (MQ=255)
                                                        gaCAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCg                                                                                        <  1:3339502/148‑1 (MQ=255)
                                                          cAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGAc                                                                                      >  2:2337940/1‑148 (MQ=255)
                                                          cAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGAc                                                                                      >  1:2222656/1‑148 (MQ=255)
                                                           aaGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACa                                                                                     <  1:2817021/148‑1 (MQ=255)
                                                           aaGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACa                                                                                     >  1:272305/1‑148 (MQ=255)
                                                           aaGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACa                                                                                     <  1:2576195/148‑1 (MQ=255)
                                                             gATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATg                                                                                   >  2:1570563/1‑148 (MQ=255)
                                                             gATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATg                                                                                   >  2:635495/1‑148 (MQ=255)
                                                              aTGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGa                                                                                  <  2:92257/148‑1 (MQ=255)
                                                              aTGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGa                                                                                  <  1:3279182/148‑1 (MQ=255)
                                                                ggtggtCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATc                                                                                <  2:1638855/148‑1 (MQ=255)
                                                                  tggtCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCtt                                                                              <  2:854441/148‑1 (MQ=255)
                                                                  tggtCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCtt                                                                              <  2:2430359/148‑1 (MQ=255)
                                                                    gtCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCtttt                                                                            >  2:785905/1‑148 (MQ=255)
                                                                        tatCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTAtt                                                                        <  2:2548311/148‑1 (MQ=255)
                                                                        tatCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTAtt                                                                        >  1:3391625/1‑148 (MQ=255)
                                                                         atCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTg                                                                       >  2:1468227/1‑148 (MQ=255)
                                                                          tCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGa                                                                      <  1:1973932/148‑1 (MQ=255)
                                                                           cGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGaa                                                                     >  2:181458/1‑148 (MQ=255)
                                                                            gATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAg                                                                    <  1:1878849/148‑1 (MQ=255)
                                                                             aTAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGa                                                                   <  1:761074/148‑1 (MQ=255)
                                                                             aTAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGa                                                                   <  1:2590112/148‑1 (MQ=255)
                                                                               aGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGACTTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATg                                                                 >  1:1621974/1‑148 (MQ=255)
                                                                               aGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATg                                                                 <  1:430183/148‑1 (MQ=255)
                                                                               aGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATg                                                                 <  2:3343542/148‑1 (MQ=255)
                                                                                gTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGt                                                                >  1:818909/1‑148 (MQ=255)
                                                                                   aCCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGc                                                             >  2:425772/1‑148 (MQ=255)
                                                                                    ccAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGcc                                                            >  1:812738/1‑148 (MQ=255)
                                                                                       gACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCcaca                                                         >  2:552303/1‑148 (MQ=255)
                                                                                         cTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACg                                                       <  2:3299302/148‑1 (MQ=255)
                                                                                          tgtgAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGc                                                      <  1:884327/148‑1 (MQ=255)
                                                                                            tgAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGccc                                                    >  1:971861/1‑148 (MQ=255)
                                                                                                tCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACt                                                >  1:855976/1‑148 (MQ=255)
                                                                                                  aTGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTaa                                              <  1:2411136/148‑1 (MQ=255)
                                                                                                  aTGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTaa                                              <  2:1524195/148‑1 (MQ=255)
                                                                                                      ttGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAAtgc                                          <  1:1808801/148‑1 (MQ=255)
                                                                                                       tGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAAtgct                                         >  1:2024091/1‑148 (MQ=255)
                                                                                                         cGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAAtgctgc                                       <  1:805018/148‑1 (MQ=255)
                                                                                                              ccATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCa                                  <  2:385985/148‑1 (MQ=255)
                                                                                                                 tCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAggg                               >  1:2203462/1‑148 (MQ=255)
                                                                                                                     gaATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCaa                           <  1:667829/148‑1 (MQ=255)
                                                                                                                     gaATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCaa                           <  1:1468227/148‑1 (MQ=255)
                                                                                                                      aaTCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAAc                          <  2:2315708/148‑1 (MQ=255)
                                                                                                                        tCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCAACACGCCCAACTAAATGCTGCCGCCAGGGTCAACAt                        >  2:1080339/1‑148 (MQ=255)
                                                                                                                         cAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATa                       >  1:1682799/1‑148 (MQ=255)
                                                                                                                         cAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATa                       >  1:1356757/1‑148 (MQ=255)
                                                                                                                           gCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATaaa                     <  2:2852814/148‑1 (MQ=255)
                                                                                                                            cGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAg                    >  2:21624/1‑148 (MQ=255)
                                                                                                                            cGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAg                    >  2:423473/1‑148 (MQ=255)
                                                                                                                             gAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGc                   <  2:2797275/148‑1 (MQ=255)
                                                                                                                              aGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGcc                  >  1:122644/1‑148 (MQ=255)
                                                                                                                               ggCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCg                 >  1:2203158/1‑148 (MQ=255)
                                                                                                                                gCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGa                >  1:490445/1‑148 (MQ=255)
                                                                                                                                  aacaaAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGc              >  2:2356061/1‑148 (MQ=255)
                                                                                                                                   acaaAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCa             >  2:2951189/1‑148 (MQ=255)
                                                                                                                                    caaAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCAt            <  2:2436038/148‑1 (MQ=255)
                                                                                                                                    caaAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCAt            <  1:3107336/148‑1 (MQ=255)
                                                                                                                                     aaaGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATc           >  1:2769192/1‑148 (MQ=255)
                                                                                                                                        gaagGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGcc        >  1:2316146/1‑148 (MQ=255)
                                                                                                                                        gaagGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGcc        <  1:275045/148‑1 (MQ=255)
                                                                                                                                        gaagGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGcc        >  2:1922384/1‑148 (MQ=255)
                                                                                                                                         aagGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCca       >  1:65638/1‑148 (MQ=255)
                                                                                                                                          agGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCcac      >  2:482110/1‑148 (MQ=255)
                                                                                                                                          agGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCcac      <  2:2237273/148‑1 (MQ=255)
                                                                                                                                          agGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCcac      <  1:3183811/148‑1 (MQ=255)
                                                                                                                                           gggAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCcaca     >  1:109167/1‑148 (MQ=255)
                                                                                                                                             gAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCACATg   <  2:598096/148‑1 (MQ=255)
                                                                                                                                              aaTTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCACATGc  <  2:3454630/148‑1 (MQ=255)
                                                                                                                                              aaTTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCACATGc  <  1:599654/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCACATGC  >  CP009273/1614692‑1614981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCACATGCTGACC  >  CP009273/1614689‑1614986
                                                                                                                                                    |                                                                                                                                                     
GTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTA                                                                                                                                                      <  SRR19973065.2250623/150‑1 (MQ=60)
   ACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTG                                                                                                                                                   <  SRR19973065.91577/150‑1 (MQ=60)
   ACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTG                                                                                                                                                   <  SRR19973065.90047/150‑1 (MQ=60)
     GTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGG                                                                                                                                                 <  SRR19973065.273920/150‑1 (MQ=60)
        CCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATT                                                                                                                                              >  SRR19973065.3314005/1‑150 (MQ=60)
        CCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATT                                                                                                                                              >  SRR19973065.3143437/1‑150 (MQ=60)
         CCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTA                                                                                                                                             >  SRR19973065.656845/1‑150 (MQ=60)
         CCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTA                                                                                                                                             >  SRR19973065.798969/1‑150 (MQ=60)
         CCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTA                                                                                                                                             >  SRR19973065.601824/1‑150 (MQ=60)
         CCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTA                                                                                                                                             <  SRR19973065.2610005/150‑1 (MQ=60)
            AGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGACTTATTGGGATTAAAG                                                                                                                                          >  SRR19973065.1558223/1‑150 (MQ=60)
            AGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAG                                                                                                                                          >  SRR19973065.329218/1‑150 (MQ=60)
             GATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGC                                                                                                                                         >  SRR19973065.2251495/1‑150 (MQ=60)
                    TAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGACTTATTGGGATTAAAGCGCTCCAG                                                                                                                                  >  SRR19973065.2182894/1‑150 (MQ=60)
                     AACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCTAGGCAACAAAGAAGGGAATTATTTGTATTAAAGCGCTCCAGA                                                                                                                                 >  SRR19973065.3325220/1‑150 (MQ=60)
                     AACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGA                                                                                                                                 >  SRR19973065.3325240/1‑150 (MQ=60)
                        CAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTA                                                                                                                              >  SRR19973065.2451403/1‑150 (MQ=60)
                            AATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAG                                                                                                                          <  SRR19973065.1726500/150‑1 (MQ=60)
                                     CACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCAC                                                                                                                 >  SRR19973065.1534193/1‑150 (MQ=60)
                                         AACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCC                                                                                                             >  SRR19973065.3260061/1‑150 (MQ=60)
                                            GCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGA                                                                                                          >  SRR19973065.2814603/1‑150 (MQ=60)
                                                GATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCG                                                                                                      <  SRR19973065.2752466/150‑1 (MQ=60)
                                                GATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCG                                                                                                      <  SRR19973065.443311/150‑1 (MQ=60)
                                                    CATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGA                                                                                                  >  SRR19973065.535578/1‑150 (MQ=60)
                                                    CATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGA                                                                                                  >  SRR19973065.536402/1‑150 (MQ=60)
                                                        AGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGC                                                                                              <  SRR19973065.2969914/150‑1 (MQ=60)
                                                        AGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGC                                                                                              <  SRR19973065.2874206/150‑1 (MQ=60)
                                                        AGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGC                                                                                              >  SRR19973065.1179987/1‑150 (MQ=60)
                                                         GAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCG                                                                                             <  SRR19973065.2662851/150‑1 (MQ=60)
                                                          AGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGA                                                                                            >  SRR19973065.3237363/1‑150 (MQ=60)
                                                          AGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGA                                                                                            >  SRR19973065.954834/1‑150 (MQ=60)
                                                           GACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGAC                                                                                           <  SRR19973065.3359900/150‑1 (MQ=60)
                                                           GACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGAC                                                                                           >  SRR19973065.2236713/1‑150 (MQ=60)
                                                           GACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGAC                                                                                           >  SRR19973065.2236836/1‑150 (MQ=60)
                                                            ACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACA                                                                                          >  SRR19973065.273975/1‑150 (MQ=60)
                                                            ACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACA                                                                                          >  SRR19973065.274141/1‑150 (MQ=60)
                                                              AAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATG                                                                                        <  SRR19973065.2592263/150‑1 (MQ=60)
                                                              AAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATG                                                                                        <  SRR19973065.2834428/150‑1 (MQ=60)
                                                               AGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGA                                                                                       >  SRR19973065.2708331/1‑150 (MQ=60)
                                                               AGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGA                                                                                       >  SRR19973065.803838/1‑150 (MQ=60)
                                                                 ATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATC                                                                                     <  SRR19973065.2602616/150‑1 (MQ=60)
                                                                 ATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATC                                                                                     <  SRR19973065.1138598/150‑1 (MQ=60)
                                                                 ATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATC                                                                                     <  SRR19973065.3299110/150‑1 (MQ=60)
                                                                      GGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTT                                                                                <  SRR19973065.199580/150‑1 (MQ=60)
                                                                         CATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATT                                                                             >  SRR19973065.3412370/1‑150 (MQ=60)
                                                                          ATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTG                                                                            >  SRR19973065.2996888/1‑150 (MQ=60)
                                                                             TCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAG                                                                         <  SRR19973065.1986785/150‑1 (MQ=60)
                                                                               GATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGAT                                                                       <  SRR19973065.1891219/150‑1 (MQ=60)
                                                                                ATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGACTTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATG                                                                      >  SRR19973065.1632846/1‑150 (MQ=60)
                                                                                ATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATG                                                                      <  SRR19973065.2606229/150‑1 (MQ=60)
                                                                                ATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATG                                                                      <  SRR19973065.766146/150‑1 (MQ=60)
                                                                                ATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATG                                                                      >  SRR19973065.1632729/1‑150 (MQ=60)
                                                                                 TAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGT                                                                     >  SRR19973065.824289/1‑150 (MQ=60)
                                                                                  AGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTT                                                                    <  SRR19973065.433168/150‑1 (MQ=60)
                                                                                     AACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCC                                                                 <  SRR19973065.1698226/150‑1 (MQ=60)
                                                                                     AACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCC                                                                 >  SRR19973065.818096/1‑150 (MQ=60)
                                                                                     AACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCC                                                                 >  SRR19973065.817119/1‑150 (MQ=60)
                                                                                     AACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCC                                                                 >  SRR19973065.2464966/1‑150 (MQ=60)
                                                                                             TGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCC                                                         <  SRR19973065.889951/150‑1 (MQ=60)
                                                                                             TGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCC                                                         >  SRR19973065.978312/1‑150 (MQ=60)
                                                                                             TGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCC                                                         >  SRR19973065.978313/1‑150 (MQ=60)
                                                                                                GAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAAC                                                      <  SRR19973065.1818145/150‑1 (MQ=60)
                                                                                                 AATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACT                                                     >  SRR19973065.861505/1‑150 (MQ=60)
                                                                                                 AATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACT                                                     >  SRR19973065.861576/1‑150 (MQ=60)
                                                                                                     ATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAAT                                                 <  SRR19973065.2426317/150‑1 (MQ=60)
                                                                                                        GTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCT                                              >  SRR19973065.2037105/1‑150 (MQ=60)
                                                                                                         TTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTG                                             <  SRR19973065.1820949/150‑1 (MQ=60)
                                                                                                         TTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTG                                             <  SRR19973065.1820704/150‑1 (MQ=60)
                                                                                                           GCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCC                                           <  SRR19973065.2194104/150‑1 (MQ=60)
                                                                                                            CGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCG                                          <  SRR19973065.810339/150‑1 (MQ=60)
                                                                                                             GGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGC                                         >  SRR19973065.2450822/1‑150 (MQ=60)
                                                                                                               AACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCA                                       >  SRR19973065.77368/1‑150 (MQ=60)
                                                                                                                 CCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGG                                     >  SRR19973065.649003/1‑150 (MQ=60)
                                                                                                                 CCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGG                                     >  SRR19973065.853305/1‑150 (MQ=60)
                                                                                                                  CATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGG                                    >  SRR19973065.2217564/1‑150 (MQ=60)
                                                                                                                       AGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAAC                               <  SRR19973065.3351073/150‑1 (MQ=60)
                                                                                                                        GAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACA                              <  SRR19973065.3529547/150‑1 (MQ=60)
                                                                                                                        GAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACA                              <  SRR19973065.1477645/150‑1 (MQ=60)
                                                                                                                        GAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACA                              <  SRR19973065.672275/150‑1 (MQ=60)
                                                                                                                         AATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACAT                             <  SRR19973065.1664411/150‑1 (MQ=60)
                                                                                                                          ATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATA                            >  SRR19973065.1694055/1‑150 (MQ=60)
                                                                                                                          ATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATA                            <  SRR19973065.753564/150‑1 (MQ=60)
                                                                                                                          ATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATA                            >  SRR19973065.1365747/1‑150 (MQ=60)
                                                                                                                          ATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATA                            >  SRR19973065.1365729/1‑150 (MQ=60)
                                                                                                                              GCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGC                        <  SRR19973065.2113698/150‑1 (MQ=60)
                                                                                                                               CGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCC                       >  SRR19973065.123713/1‑150 (MQ=60)
                                                                                                                               CGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCC                       >  SRR19973065.123609/1‑150 (MQ=60)
                                                                                                                                GAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCG                      >  SRR19973065.2217260/1‑150 (MQ=60)
                                                                                                                                GAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCG                      >  SRR19973065.2217359/1‑150 (MQ=60)
                                                                                                                                 AGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGA                     >  SRR19973065.82422/1‑150 (MQ=60)
                                                                                                                                 AGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGA                     >  SRR19973065.493755/1‑150 (MQ=60)
                                                                                                                                      ACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATC                >  SRR19973065.2786390/1‑150 (MQ=60)
                                                                                                                                      ACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATC                >  SRR19973065.2786395/1‑150 (MQ=60)
                                                                                                                                       CAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCG               <  SRR19973065.1369599/150‑1 (MQ=60)
                                                                                                                                       CAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCG               <  SRR19973065.3126290/150‑1 (MQ=60)
                                                                                                                                         AAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCC             <  SRR19973065.3384962/150‑1 (MQ=60)
                                                                                                                                         AAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCC             >  SRR19973065.2330671/1‑150 (MQ=60)
                                                                                                                                         AAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCC             >  SRR19973065.250446/1‑150 (MQ=60)
                                                                                                                                          AGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCA            >  SRR19973065.66347/1‑150 (MQ=60)
                                                                                                                                          AGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCA            >  SRR19973065.66339/1‑150 (MQ=60)
                                                                                                                                           GAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCAC           <  SRR19973065.276890/150‑1 (MQ=60)
                                                                                                                                            AAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCACA          >  SRR19973065.110155/1‑150 (MQ=60)
                                                                                                                                             AGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCACAT         <  SRR19973065.3203039/150‑1 (MQ=60)
                                                                                                                                               GGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCACATGC       >  SRR19973065.1953173/1‑150 (MQ=60)
                                                                                                                                                 AATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCACATGCTG     <  SRR19973065.603398/150‑1 (MQ=60)
                                                                                                                                                 AATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCACATGCTG     <  SRR19973065.2380396/150‑1 (MQ=60)
                                                                                                                                                  ATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCACATGCTGA    <  SRR19973065.657562/150‑1 (MQ=60)
                                                                                                                                                  ATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCACATGCTGA    <  SRR19973065.2478360/150‑1 (MQ=60)
                                                                                                                                                    TATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCACATGCTGACC  <  SRR19973065.131010/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
GTAACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCACATGCTGACC  >  CP009273/1614689‑1614986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑