Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,348,894 T→A 94.4% V384E (GTG→GAG)  glpB → sn‑glycerol‑3‑phosphate dehydrogenase (anaerobic), membrane anchor subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,348,8940TA94.4% 608.4 / 16.9 190V384E (GTG→GAG) glpBsn‑glycerol‑3‑phosphate dehydrogenase (anaerobic), membrane anchor subunit
Reads supporting (aligned to +/- strand):  ref base T (6/5);  new base A (90/89);  total (96/94)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 195 total aligned reads displayed.

CTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCAC  >  CP009273/2348749‑2349037
                                                                                                                                                 |                                                                                                                                               
cTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGc                                                                                                                                               <  1:2332222/148‑1 (MQ=255)
cTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGc                                                                                                                                               <  2:580612/148‑1 (MQ=255)
  aCAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTg                                                                                                                                             >  2:130185/1‑148 (MQ=255)
  aCAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTg                                                                                                                                             >  2:302985/1‑148 (MQ=255)
   cAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTgg                                                                                                                                            <  2:27462/148‑1 (MQ=255)
      aCCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCg                                                                                                                                         >  1:2076309/1‑148 (MQ=255)
        cGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGa                                                                                                                                       <  2:2660303/148‑1 (MQ=255)
           cACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGAttt                                                                                                                                    >  1:1368326/1‑148 (MQ=255)
           cACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGAttt                                                                                                                                    >  1:187672/1‑148 (MQ=255)
              gcgGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGAt                                                                                                                                 <  1:868363/148‑1 (MQ=255)
                 gggTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATccc                                                                                                                              <  2:1709210/148‑1 (MQ=255)
                   gTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCAt                                                                                                                            >  1:1029297/1‑148 (MQ=255)
                   gTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCAt                                                                                                                            <  2:2137553/148‑1 (MQ=255)
                   gTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCAGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCAt                                                                                                                            >  2:3162923/1‑148 (MQ=255)
                    tGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATc                                                                                                                           >  2:2239900/1‑148 (MQ=255)
                       aTGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGcc                                                                                                                        <  2:411691/148‑1 (MQ=255)
                       aTGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGcc                                                                                                                        <  1:1441182/148‑1 (MQ=255)
                       aTGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGcc                                                                                                                        <  2:3025710/148‑1 (MQ=255)
                        tGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGccc                                                                                                                       >  2:2881654/1‑148 (MQ=255)
                        tGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGccc                                                                                                                       >  2:2881666/1‑148 (MQ=255)
                           tataAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAgg                                                                                                                    <  1:613433/148‑1 (MQ=255)
                            ataAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAggg                                                                                                                   <  2:1278503/148‑1 (MQ=255)
                            ataAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAggg                                                                                                                   >  2:848375/1‑148 (MQ=255)
                            ataAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAggg                                                                                                                   <  2:2014825/148‑1 (MQ=255)
                            ataAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAggg                                                                                                                   <  1:3086979/148‑1 (MQ=255)
                            ataAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAggg                                                                                                                   <  1:289667/148‑1 (MQ=255)
                                gggAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATgc                                                                                                               >  2:1406108/1‑148 (MQ=255)
                                 ggAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATgcg                                                                                                              >  1:528216/1‑148 (MQ=255)
                                 ggAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATgcg                                                                                                              >  2:2076251/1‑148 (MQ=255)
                                  gagaTTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATgcgg                                                                                                             >  2:2313599/1‑148 (MQ=255)
                                  gagaTTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATgcgg                                                                                                             >  1:2831297/1‑148 (MQ=255)
                                  gagaTTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATgcgg                                                                                                             <  1:2379104/148‑1 (MQ=255)
                                  gagaTTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATgcgg                                                                                                             <  2:2249971/148‑1 (MQ=255)
                                    gaTTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATgcggcg                                                                                                           <  2:613921/148‑1 (MQ=255)
                                     aTTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATgcggcgg                                                                                                          >  1:1296255/1‑148 (MQ=255)
                                       ttttttGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATgcggcggcg                                                                                                        >  2:1347729/1‑148 (MQ=255)
                                              cgccgcAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTtg                                                                                                 <  2:670400/148‑1 (MQ=255)
                                                 cgcAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGc                                                                                              <  2:1426181/148‑1 (MQ=255)
                                                 cgcAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGc                                                                                              <  2:1949880/148‑1 (MQ=255)
                                                    aaCCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGt                                                                                           >  1:113060/1‑148 (MQ=255)
                                                    aaCCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACAATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGt                                                                                           >  1:3279818/1‑148 (MQ=255)
                                                         tGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCTGAGCTGGGCGTATTTGATCCAATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTg                                                                                      <  1:621692/148‑1 (MQ=255)
                                                           gcagcaGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCt                                                                                    <  2:1865950/148‑1 (MQ=255)
                                                           gcagcaGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCt                                                                                    <  1:2262579/148‑1 (MQ=255)
                                                           gcagcaGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCt                                                                                    <  1:1648092/148‑1 (MQ=255)
                                                           gcagcaGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCt                                                                                    >  1:1861949/1‑148 (MQ=255)
                                                               cagTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCAGAGGCAGGGCACACCATTGAAAAGCTGTTTGCCATCGGTTCGGTGCTGGGCGGATGTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCCGTGCTTTAc                                                                                >  2:2708838/1‑148 (MQ=255)
                                                               cagTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAGACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTAc                                                                                >  2:508640/1‑148 (MQ=255)
                                                                  ttCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATg                                                                             <  2:2085131/148‑1 (MQ=255)
                                                                       tgtAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAATACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCt                                                                        <  2:1587770/148‑1 (MQ=255)
                                                                            ccACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACa                                                                   >  2:1876816/1‑148 (MQ=255)
                                                                             cACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAg                                                                  >  2:704899/1‑148 (MQ=255)
                                                                             cACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAg                                                                  <  2:3307311/148‑1 (MQ=255)
                                                                             cACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAg                                                                  <  1:1932060/148‑1 (MQ=255)
                                                                              aCTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGa                                                                 <  1:213016/148‑1 (MQ=255)
                                                                              aCTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGa                                                                 <  2:2076309/148‑1 (MQ=255)
                                                                               cTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGAt                                                                >  2:7445/1‑148 (MQ=255)
                                                                                tgatgaGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGAtt                                                               >  1:1097473/1‑148 (MQ=255)
                                                                                 gatgaGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTg                                                              >  1:610752/1‑148 (MQ=255)
                                                                                  atgaGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGc                                                             <  1:476183/148‑1 (MQ=255)
                                                                                  atgaGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGc                                                             <  1:1821248/148‑1 (MQ=255)
                                                                                   tgaGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGcc                                                            >  2:2580364/1‑148 (MQ=255)
                                                                                      gaCGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCaa                                                         >  1:772738/1‑148 (MQ=255)
                                                                                           tACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACgcgc                                                    <  1:1450507/148‑1 (MQ=255)
                                                                                            aCGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCa                                                   <  2:1088689/148‑1 (MQ=255)
                                                                                            aCGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCa                                                   <  2:2267123/148‑1 (MQ=255)
                                                                                             cGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCag                                                  >  1:2605419/1‑148 (MQ=255)
                                                                                                 cGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCaggagg                                              <  1:3154228/148‑1 (MQ=255)
                                                                                                    cacaGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCca                                           <  2:445422/148‑1 (MQ=255)
                                                                                                     acaGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCcaa                                          >  1:1531188/1‑148 (MQ=255)
                                                                                                        ggcaggGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCcaacaa                                       >  2:1224074/1‑148 (MQ=255)
                                                                                                        ggcaggGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCcaacaa                                       <  1:3252065/148‑1 (MQ=255)
                                                                                                        ggcaggGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCcaacaa                                       <  1:3252774/148‑1 (MQ=255)
                                                                                                             ggCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACaatgaat                                  >  2:2461627/1‑148 (MQ=255)
                                                                                                             ggCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACaatgaat                                  >  1:202316/1‑148 (MQ=255)
                                                                                                               cAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAatgaatga                                >  1:1408670/1‑148 (MQ=255)
                                                                                                               cAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAatgaatga                                >  2:985049/1‑148 (MQ=255)
                                                                                                                 aaCCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGaca                              >  2:1559761/1‑148 (MQ=255)
                                                                                                                 aaCCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGaca                              >  2:569256/1‑148 (MQ=255)
                                                                                                                  aCCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGNTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGacac                             >  1:1677108/1‑148 (MQ=255)
                                                                                                                  aCCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGacac                             <  1:46546/148‑1 (MQ=255)
                                                                                                                   ccATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACAcc                            >  1:978505/1‑148 (MQ=255)
                                                                                                                   ccATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACAcc                            >  2:1909498/1‑148 (MQ=255)
                                                                                                                   ccATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACAcc                            >  1:2036440/1‑148 (MQ=255)
                                                                                                                      ttGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGc                         <  2:260829/148‑1 (MQ=255)
                                                                                                                      ttGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGc                         >  2:2556427/1‑148 (MQ=255)
                                                                                                                           aaCCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGa                    >  2:2818369/1‑148 (MQ=255)
                                                                                                                            aCCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGaa                   >  1:762167/1‑148 (MQ=255)
                                                                                                                              cTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGaaaa                 <  1:2881666/148‑1 (MQ=255)
                                                                                                                              cTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGaaaa                 <  1:1232391/148‑1 (MQ=255)
                                                                                                                                 tttGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTg              >  1:1491717/1‑148 (MQ=255)
                                                                                                                                  ttGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGc             >  1:2686939/1‑148 (MQ=255)
                                                                                                                                   tGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCTTCAGATTGCCCTACGCGCAGGTGGCCTACAATGAATGACACGAGCTTCGAAAACTGCa            <  2:1397964/148‑1 (MQ=255)
                                                                                                                                   tGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCa            <  1:302985/148‑1 (MQ=255)
                                                                                                                                      cATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTa         <  2:2934636/148‑1 (MQ=255)
                                                                                                                                      cATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTa         >  2:523803/1‑148 (MQ=255)
                                                                                                                                       aTCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTaa        >  2:93133/1‑148 (MQ=255)
                                                                                                                                       aTCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTaa        <  2:2133262/148‑1 (MQ=255)
                                                                                                                                       aTCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTaa        >  2:1239478/1‑148 (MQ=255)
                                                                                                                                             tcggaGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCAc  >  1:1596938/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCAC  >  CP009273/2348749‑2349037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCAC  >  CP009273/2348745‑2349037
                                                                                                                                                     |                                                                                                                                               
TGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGA                                                                                                                                                 <  SRR19973070.1540857/150‑1 (MQ=60)
TGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGA                                                                                                                                                 <  SRR19973070.538410/150‑1 (MQ=60)
    CTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTG                                                                                                                                             <  SRR19973070.2347711/150‑1 (MQ=60)
    CTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTG                                                                                                                                             <  SRR19973070.602332/150‑1 (MQ=60)
        AAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCG                                                                                                                                         >  SRR19973070.2090283/1‑150 (MQ=60)
             GCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTT                                                                                                                                    >  SRR19973070.189131/1‑150 (MQ=60)
             GCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTT                                                                                                                                    >  SRR19973070.262573/1‑150 (MQ=60)
             GCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTT                                                                                                                                    >  SRR19973070.1377575/1‑150 (MQ=60)
             GCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTT                                                                                                                                    >  SRR19973070.1377640/1‑150 (MQ=60)
                  GCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCC                                                                                                                               <  SRR19973070.874432/150‑1 (MQ=60)
                  GCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCC                                                                                                                               <  SRR19973070.874428/150‑1 (MQ=60)
                     GGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCAT                                                                                                                            >  SRR19973070.1036586/1‑150 (MQ=60)
                      GGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATC                                                                                                                           >  SRR19973070.1878881/1‑150 (MQ=60)
                       GTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCG                                                                                                                          <  SRR19973070.615102/150‑1 (MQ=60)
                           ATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCA                                                                                                                      <  SRR19973070.1451177/150‑1 (MQ=60)
                           ATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCA                                                                                                                      <  SRR19973070.1453985/150‑1 (MQ=60)
                              GTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGG                                                                                                                   <  SRR19973070.1613378/150‑1 (MQ=60)
                               TATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGA                                                                                                                  <  SRR19973070.617733/150‑1 (MQ=60)
                                ATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGAT                                                                                                                 <  SRR19973070.3106835/150‑1 (MQ=60)
                                ATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGAT                                                                                                                 <  SRR19973070.291435/150‑1 (MQ=60)
                                ATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGAT                                                                                                                 <  SRR19973070.291624/150‑1 (MQ=60)
                                   AGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCG                                                                                                              >  SRR19973070.531785/1‑150 (MQ=60)
                                    GGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGG                                                                                                             >  SRR19973070.2849615/1‑150 (MQ=60)
                                      GAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCG                                                                                                           <  SRR19973070.2394826/150‑1 (MQ=60)
                                       AGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGG                                                                                                          >  SRR19973070.1305277/1‑150 (MQ=60)
                                       AGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGG                                                                                                          >  SRR19973070.1856709/1‑150 (MQ=60)
                                                TGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTG                                                                                                 <  SRR19973070.2384033/150‑1 (MQ=60)
                                                      GCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGT                                                                                           >  SRR19973070.114101/1‑150 (MQ=60)
                                                      GCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACAATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCTT                                                                                           >  SRR19973070.3300889/1‑150 (MQ=60)
                                                             TGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCTGAGCTGGGCGTATTTGATCCAATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCT                                                                                    <  SRR19973070.626074/150‑1 (MQ=60)
                                                             TGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCT                                                                                    >  SRR19973070.1874684/1‑150 (MQ=60)
                                                               GCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTT                                                                                  <  SRR19973070.2810384/150‑1 (MQ=60)
                                                               GCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTT                                                                                  <  SRR19973070.2277590/150‑1 (MQ=60)
                                                               GCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTT                                                                                  <  SRR19973070.1659503/150‑1 (MQ=60)
                                                               GCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTT                                                                                  <  SRR19973070.1659472/150‑1 (MQ=60)
                                                                 AGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTAC                                                                                >  SRR19973070.1895925/1‑150 (MQ=60)
                                                                         GGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCT                                                                        >  SRR19973070.2144650/1‑150 (MQ=60)
                                                                           TNTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCA                                                                      >  SRR19973070.458035/1‑150 (MQ=60)
                                                                                CCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGA                                                                 <  SRR19973070.1246919/150‑1 (MQ=60)
                                                                                 CACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGAT                                                                <  SRR19973070.1945066/150‑1 (MQ=60)
                                                                                 CACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGAT                                                                <  SRR19973070.3169223/150‑1 (MQ=60)
                                                                                  ACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATT                                                               <  SRR19973070.3273278/150‑1 (MQ=60)
                                                                                  ACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATT                                                               >  SRR19973070.1105030/1‑150 (MQ=60)
                                                                                  ACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATT                                                               <  SRR19973070.214582/150‑1 (MQ=60)
                                                                                  ACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATT                                                               >  SRR19973070.1105033/1‑150 (MQ=60)
                                                                                   CTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTG                                                              >  SRR19973070.615038/1‑150 (MQ=60)
                                                                                      ATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCC                                                           <  SRR19973070.479546/150‑1 (MQ=60)
                                                                                      ATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCC                                                           <  SRR19973070.1833789/150‑1 (MQ=60)
                                                                                       TGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCA                                                          >  SRR19973070.3212276/1‑150 (MQ=60)
                                                                                        GAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAA                                                         >  SRR19973070.3278092/1‑150 (MQ=60)
                                                                                        GAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAA                                                         >  SRR19973070.778137/1‑150 (MQ=60)
                                                                                        GAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAA                                                         >  SRR19973070.778155/1‑150 (MQ=60)
                                                                                               TACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAG                                                  >  SRR19973070.2622430/1‑150 (MQ=60)
                                                                                               TACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAG                                                  <  SRR19973070.1460595/150‑1 (MQ=60)
                                                                                                 CGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGA                                                >  SRR19973070.479511/1‑150 (MQ=60)
                                                                                                   CCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGTCGCTCAACAGATTGCCCAACGCGCAGGAGG                                              >  SRR19973070.498453/1‑150 (MQ=60)
                                                                                                   CCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGG                                              <  SRR19973070.854177/150‑1 (MQ=60)
                                                                                                     CGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCC                                            >  SRR19973070.2000693/1‑150 (MQ=60)
                                                                                                     CGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCC                                            <  SRR19973070.3174609/150‑1 (MQ=60)
                                                                                                       TCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAA                                          >  SRR19973070.1541953/1‑150 (MQ=60)
                                                                                                       TCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAA                                          >  SRR19973070.1541940/1‑150 (MQ=60)
                                                                                                        CACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAAC                                         <  SRR19973070.3091784/150‑1 (MQ=60)
                                                                                                            GGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATA                                     <  SRR19973070.3273018/150‑1 (MQ=60)
                                                                                                            GGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATA                                     <  SRR19973070.3273728/150‑1 (MQ=60)
                                                                                                               AGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAAT                                  >  SRR19973070.2070917/1‑150 (MQ=60)
                                                                                                               AGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAAT                                  >  SRR19973070.203844/1‑150 (MQ=60)
                                                                                                               AGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAAT                                  >  SRR19973070.204100/1‑150 (MQ=60)
                                                                                                                 GGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGCGGCCAACAATGAATGA                                >  SRR19973070.1418274/1‑150 (MQ=60)
                                                                                                                 GGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGA                                >  SRR19973070.1418256/1‑150 (MQ=60)
                                                                                                                    AAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGNTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACAC                             >  SRR19973070.1688588/1‑150 (MQ=60)
                                                                                                                    AAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACAC                             >  SRR19973070.2539352/1‑150 (MQ=60)
                                                                                                                     AACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACC                            >  SRR19973070.985489/1‑150 (MQ=60)
                                                                                                                     AACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACC                            >  SRR19973070.2050217/1‑150 (MQ=60)
                                                                                                                     AACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACC                            >  SRR19973070.1787123/1‑150 (MQ=60)
                                                                                                                      ACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCA                           <  SRR19973070.47134/150‑1 (MQ=60)
                                                                                                                        CATTGAAAACCTGTTAGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGC                         >  SRR19973070.1225945/1‑150 (MQ=60)
                                                                                                                              AAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAA                   >  SRR19973070.767521/1‑150 (MQ=60)
                                                                                                                                ACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAA                 <  SRR19973070.2306959/150‑1 (MQ=60)
                                                                                                                                  CTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACT               <  SRR19973070.1241022/150‑1 (MQ=60)
                                                                                                                                  CTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACT               <  SRR19973070.2900310/150‑1 (MQ=60)
                                                                                                                                  CTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACT               <  SRR19973070.2900322/150‑1 (MQ=60)
                                                                                                                                   TGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTG              >  SRR19973070.1501656/1‑150 (MQ=60)
                                                                                                                                   TGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTG              >  SRR19973070.1503954/1‑150 (MQ=60)
                                                                                                                                   TGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTG              >  SRR19973070.1502156/1‑150 (MQ=60)
                                                                                                                                    GTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGC             >  SRR19973070.2704357/1‑150 (MQ=60)
                                                                                                                                       TGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATT          <  SRR19973070.305069/150‑1 (MQ=60)
                                                                                                                                       TGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATT          <  SRR19973070.305118/150‑1 (MQ=60)
                                                                                                                                           ATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGT      <  SRR19973070.1415655/150‑1 (MQ=60)
                                                                                                                                               GTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCAC  <  SRR19973070.653971/150‑1 (MQ=60)
                                                                                                                                               GTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCAC  >  SRR19973070.1608097/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                               
TGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCAC  >  CP009273/2348745‑2349037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑