Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F159 I0 R1 879 305.9 9588012 96.6% 9262019 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,348,894 T→A 36.7% V384E (GTG→GAG)  glpB → sn‑glycerol‑3‑phosphate dehydrogenase (anaerobic), membrane anchor subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,348,8940TA36.7% 396.5 / inf 357V384E (GTG→GAG) glpBsn‑glycerol‑3‑phosphate dehydrogenase (anaerobic), membrane anchor subunit
Reads supporting (aligned to +/- strand):  ref base T (102/123);  new base A (68/63);  total (170/187)
Fisher's exact test for biased strand distribution p-value = 2.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 378 total aligned reads displayed.

GCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCACCGTCTG  >  CP009273/2348748‑2349043
                                                                                                                                                  |                                                                                                                                                     
  tACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTgg                                                                                                                                                  <  1:1267860/150‑1 (MQ=255)
     aaaCCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCg                                                                                                                                               >  1:2298630/1‑150 (MQ=255)
        ccGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTAGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGAt                                                                                                                                            >  2:921813/1‑150 (MQ=255)
           ccACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTg                                                                                                                                         <  1:1832939/150‑1 (MQ=255)
              cgcgGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATc                                                                                                                                      >  2:2742717/1‑150 (MQ=255)
               gcgGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATcc                                                                                                                                     <  2:459322/150‑1 (MQ=255)
                cgGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATccc                                                                                                                                    >  1:3436674/1‑150 (MQ=255)
                 ggggTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCa                                                                                                                                   <  1:968875/150‑1 (MQ=255)
                 ggggTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCa                                                                                                                                   <  1:4717390/150‑1 (MQ=255)
                   ggTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATc                                                                                                                                 <  2:3635994/150‑1 (MQ=255)
                   ggTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATc                                                                                                                                 <  2:2995958/150‑1 (MQ=255)
                    gTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCg                                                                                                                                >  1:1858951/1‑150 (MQ=255)
                    gTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCg                                                                                                                                <  2:3963882/150‑1 (MQ=255)
                      gAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGcc                                                                                                                              >  1:2779117/1‑150 (MQ=255)
                            tataAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGa                                                                                                                        <  2:1281084/150‑1 (MQ=255)
                             ataAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGAt                                                                                                                       >  2:4196808/1‑150 (MQ=255)
                             ataAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGAt                                                                                                                       <  2:3257037/150‑1 (MQ=255)
                             ataAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGAt                                                                                                                       >  2:2549450/1‑150 (MQ=255)
                               aaGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATgc                                                                                                                     <  1:806545/150‑1 (MQ=255)
                                aGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATgcg                                                                                                                    >  2:3165623/1‑150 (MQ=255)
                                 gggAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATgcgg                                                                                                                   <  2:2042540/150‑1 (MQ=255)
                                  ggAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATgcggc                                                                                                                  >  2:2624172/1‑150 (MQ=255)
                                   gagaTTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATgcggcg                                                                                                                 >  2:4775260/1‑150 (MQ=255)
                                        ttttttGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGgt                                                                                                            <  2:3079933/150‑1 (MQ=255)
                                           tttGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGtt                                                                                                         <  1:2255209/150‑1 (MQ=255)
                                            ttGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGttt                                                                                                        >  2:4086373/1‑150 (MQ=255)
                                            ttGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGttt                                                                                                        <  1:2204117/150‑1 (MQ=255)
                                            ttGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGttt                                                                                                        <  2:4295790/150‑1 (MQ=255)
                                             cgcgcCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTtg                                                                                                       <  2:1807649/149‑1 (MQ=255)
                                               cgccgcAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTtgtg                                                                                                     >  1:353364/1‑150 (MQ=255)
                                               cgccgcAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGAGTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTAtgtg                                                                                                     >  1:1573570/1‑150 (MQ=255)
                                                 ccgcAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGcc                                                                                                   <  1:3414744/150‑1 (MQ=255)
                                                   gcAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGt                                                                                                 >  2:4741735/1‑150 (MQ=255)
                                                     aaCCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCa                                                                                               >  2:2417829/1‑150 (MQ=255)
                                                      aCCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAg                                                                                              >  1:87531/1‑150 (MQ=255)
                                                      aCCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAg                                                                                              >  2:4146800/1‑150 (MQ=255)
                                                       ccGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGt                                                                                             <  2:4156461/150‑1 (MQ=255)
                                                       ccGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGt                                                                                             <  1:4415663/150‑1 (MQ=255)
                                                        cGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGTATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTg                                                                                            <  1:2466160/150‑1 (MQ=255)
                                                         gTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGc                                                                                           >  2:2328506/1‑150 (MQ=255)
                                                           ggCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACATGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCtt                                                                                         >  1:628603/1‑150 (MQ=255)
                                                             cagcagTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTa                                                                                       <  2:564959/150‑1 (MQ=255)
                                                             cagcagTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTa                                                                                       <  1:503009/150‑1 (MQ=255)
                                                              agcagTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTAc                                                                                      >  2:440134/1‑150 (MQ=255)
                                                              agcagTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTAc                                                                                      <  2:2909604/150‑1 (MQ=255)
                                                               gcagTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACa                                                                                     <  1:1778671/150‑1 (MQ=255)
                                                                cagTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACAt                                                                                    <  1:1408219/150‑1 (MQ=255)
                                                                 agTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATg                                                                                   >  1:2866831/1‑150 (MQ=255)
                                                                 agTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATg                                                                                   >  2:2386449/1‑150 (MQ=255)
                                                                  gTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGc                                                                                  >  1:2571518/1‑150 (MQ=255)
                                                                       gtgtAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTc                                                                             >  1:3885107/1‑150 (MQ=255)
                                                                       gtgtAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTc                                                                             <  1:1589569/150‑1 (MQ=255)
                                                                       gtgtAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTc                                                                             <  1:1652944/150‑1 (MQ=255)
                                                                        tgtAACCACTGATGAGACGCGACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCa                                                                            <  2:1636689/150‑1 (MQ=255)
                                                                           aaCCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACa                                                                         >  1:2814228/1‑150 (MQ=255)
                                                                            aCCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAg                                                                        >  2:1511281/1‑150 (MQ=255)
                                                                                cTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTg                                                                    >  2:3884064/1‑150 (MQ=255)
                                                                                    tgaGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCa                                                                <  2:4260431/150‑1 (MQ=255)
                                                                                    tgaGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCa                                                                <  1:3026286/150‑1 (MQ=255)
                                                                                    tgaGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCa                                                                <  2:2224761/150‑1 (MQ=255)
                                                                                      agaCGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAAc                                                              <  2:342371/150‑1 (MQ=255)
                                                                                            tACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCag                                                        >  2:1929477/1‑150 (MQ=255)
                                                                                            tACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCag                                                        >  2:708490/1‑150 (MQ=255)
                                                                                               gCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCaggag                                                     <  1:2615878/150‑1 (MQ=255)
                                                                                                cccGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCaggagg                                                    <  1:2647258/150‑1 (MQ=255)
                                                                                                     cacaGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCcaac                                               >  1:3718374/1‑150 (MQ=255)
                                                                                                     cacaGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCcaac                                               >  1:477285/1‑150 (MQ=255)
                                                                                                      acaGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCcaaca                                              <  1:4241943/150‑1 (MQ=255)
                                                                                                        aggcaggGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACaat                                            >  1:2693781/1‑150 (MQ=255)
                                                                                                         ggcaggGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACaatg                                           <  2:524640/150‑1 (MQ=255)
                                                                                                         ggcaggGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACaatg                                           >  2:1714267/1‑150 (MQ=255)
                                                                                                             gggCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACaatgaatg                                       <  1:1611026/150‑1 (MQ=255)
                                                                                                              ggCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAatgaatga                                      <  1:3269465/150‑1 (MQ=255)
                                                                                                               gCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGac                                     <  1:3455814/150‑1 (MQ=255)
                                                                                                                 aaaCCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGacac                                   <  1:4190664/150‑1 (MQ=255)
                                                                                                                   aCCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCa                                 <  2:2878507/150‑1 (MQ=255)
                                                                                                                   aCCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCa                                 <  1:4610102/150‑1 (MQ=255)
                                                                                                                    ccATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAg                                >  1:1025099/1‑150 (MQ=255)
                                                                                                                    ccATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAg                                <  1:3349923/150‑1 (MQ=255)
                                                                                                                        tGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTc                            <  2:802906/150‑1 (MQ=255)
                                                                                                                        tGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTc                            <  1:1152020/150‑1 (MQ=255)
                                                                                                                        tGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTc                            >  1:398777/1‑150 (MQ=255)
                                                                                                                         gAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCg                           >  1:363682/1‑150 (MQ=255)
                                                                                                                           aaaCCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGaa                         >  2:220949/1‑150 (MQ=255)
                                                                                                                            aaCCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGaaa                        <  2:3499494/150‑1 (MQ=255)
                                                                                                                            aaCCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGaaa                        <  2:1093017/150‑1 (MQ=255)
                                                                                                                            aaCCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGaaa                        >  2:4063111/1‑150 (MQ=255)
                                                                                                                              ccTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAAc                      <  1:2189279/150‑1 (MQ=255)
                                                                                                                              ccTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAAc                      >  2:2109071/1‑150 (MQ=255)
                                                                                                                               cTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACt                     <  2:1389899/150‑1 (MQ=255)
                                                                                                                               cTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACt                     <  1:2417744/150‑1 (MQ=255)
                                                                                                                                    tGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCAtt                <  1:1976301/150‑1 (MQ=255)
                                                                                                                                     gCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTa               <  2:2778006/150‑1 (MQ=255)
                                                                                                                                     gCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTa               <  2:1844799/150‑1 (MQ=255)
                                                                                                                                       cATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAg             <  1:2765267/150‑1 (MQ=255)
                                                                                                                                         tcggttcggaGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTg           <  2:3780333/150‑1 (MQ=255)
                                                                                                                                              tcggtGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCACCg      >  1:4280781/1‑150 (MQ=255)
                                                                                                                                              tcggtGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCACCg      <  1:4086373/150‑1 (MQ=255)
                                                                                                                                              tcggaGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCACCg      >  1:523503/1‑150 (MQ=255)
                                                                                                                                               cggtGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCACCGt     <  2:1270347/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCACCGTCTG  >  CP009273/2348748‑2349043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A